AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023630.t1 MTR_8g087770 86.303 679 87 3 1 678 1 674 0.0 1192
MS.gene023630.t1 MTR_8g087750 82.712 671 111 3 6 675 11 677 0.0 1129
MS.gene023630.t1 MTR_8g087763 88.444 225 26 0 374 598 4 228 5.89e-144 423
MS.gene023630.t1 MTR_4g093750 34.739 403 234 14 67 452 35 425 7.48e-43 165
MS.gene023630.t1 MTR_4g093750 40.559 286 148 9 67 337 35 313 6.79e-42 163
MS.gene023630.t1 MTR_2g009690 37.580 314 184 6 63 366 40 351 1.74e-41 162
MS.gene023630.t1 MTR_5g009150 30.128 468 291 15 69 518 35 484 2.85e-34 139
MS.gene023630.t1 MTR_1g032550 35.336 283 163 7 69 337 35 311 3.25e-33 135
MS.gene023630.t1 MTR_8g105580 35.461 282 163 6 69 337 35 310 1.08e-32 134
MS.gene023630.t1 MTR_8g105580 35.461 282 163 6 69 337 35 310 1.09e-32 134
MS.gene023630.t1 MTR_8g099550 33.956 321 178 10 69 369 34 340 9.11e-32 131
MS.gene023630.t1 MTR_7g451010 34.700 317 181 9 69 369 40 346 9.71e-32 131
MS.gene023630.t1 MTR_8g009510 35.220 318 178 10 69 369 40 346 1.27e-31 131
MS.gene023630.t1 MTR_4g098920 34.375 320 178 11 69 369 34 340 6.53e-31 128
MS.gene023630.t1 MTR_4g085860 76.316 76 17 1 206 280 2 77 7.10e-31 115
MS.gene023630.t1 MTR_8g027675 41.709 199 105 5 69 264 34 224 1.00e-30 128
MS.gene023630.t1 MTR_6g045107 75.000 76 18 1 206 280 2 77 6.06e-29 110
MS.gene023630.t1 MTR_8g009510 31.452 248 146 7 136 369 2 239 5.46e-17 85.1
MS.gene023630.t1 MTR_8g463270 29.258 229 147 5 54 269 27 253 8.64e-16 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023630.t1 AT1G60500 57.292 672 278 5 3 671 2 667 0.0 779
MS.gene023630.t1 AT1G60530 72.069 290 73 1 18 307 19 300 4.34e-144 422
MS.gene023630.t1 AT2G14120 30.180 444 287 10 25 452 1 437 5.81e-40 157
MS.gene023630.t1 AT2G14120 30.180 444 287 10 25 452 1 437 8.71e-40 157
MS.gene023630.t1 AT2G14120 30.180 444 287 10 25 452 1 437 9.55e-40 157
MS.gene023630.t1 AT2G14120 34.125 337 204 6 25 346 1 334 1.12e-39 156
MS.gene023630.t1 AT4G33650 29.797 443 267 11 35 452 29 452 2.03e-39 155
MS.gene023630.t1 AT4G33650 29.797 443 267 11 35 452 29 452 2.08e-39 155
MS.gene023630.t1 AT1G14830 32.903 310 196 6 69 369 35 341 8.02e-34 137
MS.gene023630.t1 AT5G42080 30.258 466 293 13 69 518 34 483 6.83e-33 134
MS.gene023630.t1 AT5G42080 30.258 466 293 13 69 518 34 483 6.83e-33 134
MS.gene023630.t1 AT2G44590 24.307 613 406 19 69 665 35 605 8.71e-33 134
MS.gene023630.t1 AT3G60190 33.232 328 199 9 69 386 40 357 1.30e-32 134
MS.gene023630.t1 AT5G42080 34.505 313 187 8 69 369 34 340 4.65e-32 129
MS.gene023630.t1 AT5G42080 34.505 313 187 8 69 369 34 340 1.23e-31 130
MS.gene023630.t1 AT3G61760 34.472 322 175 12 69 369 34 340 4.52e-30 124
MS.gene023630.t1 AT3G61760 34.472 322 175 12 69 369 34 340 4.93e-30 126
MS.gene023630.t1 AT3G61760 34.472 322 175 12 69 369 34 340 6.00e-30 125
MS.gene023630.t1 AT2G44590 22.838 613 398 20 69 665 35 588 4.72e-23 104
MS.gene023630.t1 AT2G44590 28.875 329 195 10 69 386 35 335 3.09e-22 102
MS.gene023630.t1 AT1G53140 27.119 236 160 5 44 269 36 269 9.77e-15 78.6

Find 125 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTTTCAACAAAATTAAT+TGG 0.173861 4.4:-13128644 None:intergenic
CTTGAGGAAGCCAAATTTAT+TGG 0.191059 4.4:+13128548 MS.gene023630:CDS
ATTCGGAAGACCAATAAATT+TGG 0.194065 4.4:-13128558 None:intergenic
ATGGACAGGATTGGAAATTA+TGG 0.215755 4.4:+13129274 MS.gene023630:CDS
ATCAAGTTTGTTTGCCTTTG+TGG 0.244719 4.4:-13127292 None:intergenic
TATTGTTGGAATTCCTGTTT+TGG 0.276334 4.4:+13128178 MS.gene023630:CDS
GAGGAGTCTCCTTCAATATC+AGG 0.303692 4.4:+13129184 MS.gene023630:CDS
ATCTGATCATAAATGTTATC+AGG 0.304162 4.4:-13127831 None:intergenic
AGGCCAGATTGACAGCTTAT+TGG 0.313028 4.4:+13129020 MS.gene023630:CDS
TTCTAACTATTATCAGCTTC+AGG 0.313313 4.4:+13128715 MS.gene023630:CDS
GAACTTTGATTTCCTCCATT+AGG 0.316135 4.4:-13128526 None:intergenic
TAACATTTATGATCAGATTA+AGG 0.317964 4.4:+13127836 MS.gene023630:CDS
CTTCCAACCATTGTTGTTGT+TGG 0.322456 4.4:+13127474 MS.gene023630:CDS
AGAGACCGTGGCCAACATTA+TGG 0.325375 4.4:+13129255 MS.gene023630:CDS
TCACAAACTGTTGATAAAAC+TGG 0.334047 4.4:+13127954 MS.gene023630:CDS
TCCACAGCCAGAGCTTGTTT+TGG 0.338480 4.4:+13127614 MS.gene023630:CDS
CCTTACTTCAGAGAAAAGTT+AGG 0.351453 4.4:+13128606 MS.gene023630:CDS
TGAAAGTGTATGGAATTATC+TGG 0.352378 4.4:+13128658 MS.gene023630:CDS
CTTACTATGGTTGATCTTCC+AGG 0.352429 4.4:+13127780 MS.gene023630:CDS
TAAGAAGATTAATGAGAAAC+TGG 0.360082 4.4:+13128259 MS.gene023630:CDS
CAAGGGTACCTTTGGTTATG+AGG 0.366182 4.4:+13127574 MS.gene023630:CDS
TGGACAGGATTGGAAATTAT+GGG 0.366857 4.4:+13129275 MS.gene023630:CDS
CACATGTAACCCTGAGTACT+TGG 0.369075 4.4:+13128838 MS.gene023630:CDS
GCAGTTCTTGGCATGAAATT+CGG 0.376721 4.4:-13128575 None:intergenic
GCCTGCTAATTTATCCTCAC+TGG 0.379029 4.4:+13128310 MS.gene023630:CDS
TGGAGTTCCTGATCTTACTA+TGG 0.379945 4.4:+13127767 MS.gene023630:CDS
GCAGCCACTGAGGAGCTTGC+TGG 0.382789 4.4:+13127693 MS.gene023630:CDS
CTCCATTAGGAAATCCTTAG+TGG 0.385218 4.4:-13128513 None:intergenic
TAAATCTCCTGAAGGTTTGC+TGG 0.387244 4.4:+13128010 MS.gene023630:CDS
TTCAAAATAATACTCTCTTC+AGG 0.392099 4.4:-13127882 None:intergenic
GCTCTGGCTGTGGAAGTAAA+TGG 0.394731 4.4:-13127605 None:intergenic
AACAATAGATTTGTCTATCC+TGG 0.395168 4.4:-13128162 None:intergenic
ATAACTCGTGTTCCTGTTCA+TGG 0.396246 4.4:+13127804 MS.gene023630:CDS
ACCTTCTCCAGCAAACCTTC+AGG 0.399453 4.4:-13128017 None:intergenic
TTTCCAATAAGCTGTCAATC+TGG 0.399717 4.4:-13129023 None:intergenic
TTACTACTTGTGAGTCGATA+AGG 0.405010 4.4:+13127928 MS.gene023630:CDS
TGTCTCTTCCCTGATATTGA+AGG 0.410432 4.4:-13129193 None:intergenic
TTAATCTTCTTAATGATCTC+TGG 0.413053 4.4:-13128248 None:intergenic
GACGATGTTAACATTGGTCT+TGG 0.414913 4.4:+13128047 MS.gene023630:CDS
TTGATGCTCTTGAAAATCTA+AGG 0.416842 4.4:+13127421 MS.gene023630:CDS
AATTTGTACAAGGGTACCTT+TGG 0.424069 4.4:+13127566 MS.gene023630:CDS
AAGGTCAGAAAAGCAGTTCT+TGG 0.424321 4.4:-13128587 None:intergenic
GTCTTGGTTATGTTTGTGTA+AGG 0.435750 4.4:+13128063 MS.gene023630:CDS
ATATCCACACATGTTGAATC+AGG 0.440980 4.4:+13128985 MS.gene023630:CDS
TGGGGTGTTGGAAATCCCTT+TGG 0.443439 4.4:-13127721 None:intergenic
CTTACTTCAGAGAAAAGTTA+GGG 0.447328 4.4:+13128607 MS.gene023630:CDS
GGCTACTAGTTTGCATGAGC+TGG 0.448592 4.4:+13128280 MS.gene023630:CDS
AGGATAGACAAATCTATTGT+TGG 0.453655 4.4:+13128164 MS.gene023630:CDS
TCATTATAAGAAGATACAAT+TGG 0.458646 4.4:-13127381 None:intergenic
GGAAGAGACAGAAGTTGAGC+AGG 0.460748 4.4:+13129206 MS.gene023630:CDS
GAATCAGGCTTATGATTTGA+AGG 0.463037 4.4:+13129000 MS.gene023630:CDS
ACAATTGGTGCAACAGATAG+AGG 0.467988 4.4:-13127366 None:intergenic
TCTTTACTATCAAAGTCAGT+GGG 0.470875 4.4:-13127740 None:intergenic
TTATACTCCTCCAAGTACTC+AGG 0.473801 4.4:-13128848 None:intergenic
TGATACCAGCTAATGATTCA+AGG 0.474056 4.4:-13127524 None:intergenic
GAGCTTGCTGGCACTGCCAA+AGG 0.478600 4.4:+13127705 MS.gene023630:CDS
TCCTGAAGGTTTGCTGGAGA+AGG 0.484851 4.4:+13128016 MS.gene023630:CDS
GTTGTTGGAGATCAATCATC+TGG 0.485196 4.4:+13127489 MS.gene023630:CDS
AGCTTGAAGCACATGCTGAA+TGG 0.486139 4.4:-13128789 None:intergenic
TTATACTCCAAAACAAGCTC+TGG 0.492061 4.4:-13127621 None:intergenic
TATCAAAGTCAGTGGGGTGT+TGG 0.492352 4.4:-13127733 None:intergenic
TCCAATCCTGTCCATAATGT+TGG 0.494024 4.4:-13129266 None:intergenic
TTATCAGGTTGACCATGAAC+AGG 0.495576 4.4:-13127816 None:intergenic
GCGTCTCAACATTGCTAAAG+AGG 0.501157 4.4:+13127443 MS.gene023630:CDS
TGCCACTAAGGATTTCCTAA+TGG 0.506730 4.4:+13128511 MS.gene023630:CDS
TGTGTGGATATTTCATAAGC+TGG 0.507052 4.4:-13128973 None:intergenic
GTTTGTGTAAGGAATCGAAT+TGG 0.512590 4.4:+13128074 MS.gene023630:CDS
CTTATTGGAAAATTGTGCTC+CGG 0.520290 4.4:+13129035 MS.gene023630:CDS
GCCAGTGAGGATAAATTAGC+AGG 0.522213 4.4:-13128311 None:intergenic
AGATCAACCATAGTAAGATC+AGG 0.522883 4.4:-13127774 None:intergenic
AGTGTCCTTGAATCATTAGC+TGG 0.523168 4.4:+13127519 MS.gene023630:CDS
TCCAAAACAAGCTCTGGCTG+TGG 0.524796 4.4:-13127615 None:intergenic
GGTGCAACAGATAGAGGTTG+AGG 0.525331 4.4:-13127360 None:intergenic
TGTTGAAAGTAATGCCACTA+AGG 0.528407 4.4:+13128499 MS.gene023630:CDS
GCCAACATTATGGACAGGAT+TGG 0.530993 4.4:+13129265 MS.gene023630:CDS
AATGAACTGTTATCAGCAAA+TGG 0.537861 4.4:+13129139 MS.gene023630:CDS
ATCAGCTTGCCACGTGGACA+AGG 0.540401 4.4:+13127543 MS.gene023630:CDS
TCAGATTAAGGATATTATCA+TGG 0.543580 4.4:+13127848 MS.gene023630:CDS
GGAGGAAATCAAAGTTCTTG+AGG 0.547046 4.4:+13128532 MS.gene023630:CDS
AGCTTGCTGGCACTGCCAAA+GGG 0.547647 4.4:+13127706 MS.gene023630:CDS
TCTCCAACAACAACAATGGT+TGG 0.551269 4.4:-13127477 None:intergenic
TGTGAATGTTGATCTGCAAA+AGG 0.555960 4.4:+13129108 MS.gene023630:CDS
AGTGTCACACACTTCTGTCC+AGG 0.565122 4.4:+13128144 MS.gene023630:CDS
ACTTTGATAGTAAAGAAGAA+TGG 0.569006 4.4:+13127747 MS.gene023630:CDS
TCAAGTACTGTGAAGCTGAA+AGG 0.569995 4.4:+13128929 MS.gene023630:CDS
CACTAAGGATTTCCTAATGG+AGG 0.576778 4.4:+13128514 MS.gene023630:CDS
AGTGCCAGCAAGCTCCTCAG+TGG 0.578957 4.4:-13127697 None:intergenic
TTCTTTACTATCAAAGTCAG+TGG 0.579933 4.4:-13127741 None:intergenic
TATACTCCTCCAAGTACTCA+GGG 0.581284 4.4:-13128847 None:intergenic
CCGTGGCCAACATTATGGAC+AGG 0.581354 4.4:+13129260 MS.gene023630:CDS
TGAAAAGAAAATGCATTGCA+CGG 0.584106 4.4:+13128427 MS.gene023630:CDS
CAATCACATCAATTAACCTC+CGG 0.585742 4.4:-13129054 None:intergenic
CGTCTCAACATTGCTAAAGA+GGG 0.585949 4.4:+13127444 MS.gene023630:CDS
AGGAGTCTCCTTCAATATCA+GGG 0.586223 4.4:+13129185 MS.gene023630:CDS
ATGTAACCCTGAGTACTTGG+AGG 0.589927 4.4:+13128841 MS.gene023630:CDS
CCTGTCCATAATGTTGGCCA+CGG 0.590976 4.4:-13129260 None:intergenic
GTGGACAAGGAATTTGTACA+AGG 0.592993 4.4:+13127556 MS.gene023630:CDS
GGACAGGATTGGAAATTATG+GGG 0.597404 4.4:+13129276 MS.gene023630:CDS
AAGGCTGATAAATCTCCTGA+AGG 0.599516 4.4:+13128002 MS.gene023630:CDS
CAAACAACTTTCAACTGATG+AGG 0.601066 4.4:+13127647 MS.gene023630:CDS
AACGCTTGCTGTCGTGACAA+AGG 0.611997 4.4:+13127983 MS.gene023630:CDS
ACAGGAACACGAGTTATACC+TGG 0.615115 4.4:-13127798 None:intergenic
TGGTGTTGAGAGGCTGCTTG+AGG 0.618613 4.4:+13129165 MS.gene023630:CDS
TGTGCTTCAAGCTGTTGAGA+TGG 0.619725 4.4:+13128799 MS.gene023630:CDS
TAAGCCTGATTCAACATGTG+TGG 0.620737 4.4:-13128989 None:intergenic
ATTGGAAAATTGTGCTCCGG+AGG 0.621567 4.4:+13129038 MS.gene023630:CDS
GGAGGAGTATAACAAGCTGA+TGG 0.621625 4.4:+13128859 MS.gene023630:CDS
TGCTATAAACGCAGCCACTG+AGG 0.623567 4.4:+13127683 MS.gene023630:CDS
ACAGCTGACGATGTTAACAT+TGG 0.623909 4.4:+13128041 MS.gene023630:CDS
AAGAGTCTTACGAAGATGCA+AGG 0.624326 4.4:+13128099 MS.gene023630:CDS
GATTTGCAGAAAAGCAGACA+TGG 0.624535 4.4:-13128339 None:intergenic
CTGTTATCAGCAAATGGTGG+TGG 0.626367 4.4:+13129145 MS.gene023630:CDS
CAAATGGTGGTGGTGTTGAG+AGG 0.637671 4.4:+13129155 MS.gene023630:CDS
ACTGTGAAGCTGAAAGGTGT+TGG 0.640769 4.4:+13128935 MS.gene023630:CDS
GAAAGAAAGCAAAGAGACCG+TGG 0.645229 4.4:+13129243 MS.gene023630:CDS
TTGATCTCCAACAACAACAA+TGG 0.648908 4.4:-13127481 None:intergenic
GTACAAATTCCTTGTCCACG+TGG 0.660130 4.4:-13127552 None:intergenic
AGACATGGCATCAGCCAGTG+AGG 0.673951 4.4:-13128324 None:intergenic
CTTTACTATCAAAGTCAGTG+GGG 0.697240 4.4:-13127739 None:intergenic
GCTGGTATCAGCTTGCCACG+TGG 0.699523 4.4:+13127537 MS.gene023630:CDS
GTTGATCATCAACATCAACA+TGG 0.706155 4.4:-13127338 None:intergenic
TGGAAACAAGAGTTCCACAA+AGG 0.707464 4.4:+13127278 MS.gene023630:CDS
TTACTTCAGAGAAAAGTTAG+GGG 0.725881 4.4:+13128608 MS.gene023630:CDS
TGGACAAGGAATTTGTACAA+GGG 0.728664 4.4:+13127557 MS.gene023630:CDS
GAACTGTTATCAGCAAATGG+TGG 0.747464 4.4:+13129142 MS.gene023630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACACTTTCAACAAAATTAAT+TGG - chr4.4:13128647-13128666 None:intergenic 20.0%
!! CTGAAAAAAATTCTTCTTAT+AGG + chr4.4:13128383-13128402 MS.gene023630:CDS 20.0%
!! TAACATTTATGATCAGATTA+AGG + chr4.4:13127836-13127855 MS.gene023630:CDS 20.0%
!! TCATTATAAGAAGATACAAT+TGG - chr4.4:13127384-13127403 None:intergenic 20.0%
!!! TTAATTTTGTTGAAAGTGTA+TGG + chr4.4:13128648-13128667 MS.gene023630:CDS 20.0%
! ATCTGATCATAAATGTTATC+AGG - chr4.4:13127834-13127853 None:intergenic 25.0%
! TAAGAAGATTAATGAGAAAC+TGG + chr4.4:13128259-13128278 MS.gene023630:CDS 25.0%
! TCAGATTAAGGATATTATCA+TGG + chr4.4:13127848-13127867 MS.gene023630:CDS 25.0%
! TTAATCTTCTTAATGATCTC+TGG - chr4.4:13128251-13128270 None:intergenic 25.0%
! TTCAAAATAATACTCTCTTC+AGG - chr4.4:13127885-13127904 None:intergenic 25.0%
!! ACTTTGATAGTAAAGAAGAA+TGG + chr4.4:13127747-13127766 MS.gene023630:CDS 25.0%
!! TCTTTTTCTTCTTAGCAATA+AGG - chr4.4:13128766-13128785 None:intergenic 25.0%
AACAATAGATTTGTCTATCC+TGG - chr4.4:13128165-13128184 None:intergenic 30.0%
AATGAACTGTTATCAGCAAA+TGG + chr4.4:13129139-13129158 MS.gene023630:CDS 30.0%
AGGATAGACAAATCTATTGT+TGG + chr4.4:13128164-13128183 MS.gene023630:CDS 30.0%
ATTCGGAAGACCAATAAATT+TGG - chr4.4:13128561-13128580 None:intergenic 30.0%
CTTACTTCAGAGAAAAGTTA+GGG + chr4.4:13128607-13128626 MS.gene023630:CDS 30.0%
TCTTTACTATCAAAGTCAGT+GGG - chr4.4:13127743-13127762 None:intergenic 30.0%
TGAAAAGAAAATGCATTGCA+CGG + chr4.4:13128427-13128446 MS.gene023630:CDS 30.0%
TTACTTCAGAGAAAAGTTAG+GGG + chr4.4:13128608-13128627 MS.gene023630:CDS 30.0%
TTCTAACTATTATCAGCTTC+AGG + chr4.4:13128715-13128734 MS.gene023630:CDS 30.0%
TTCTTTACTATCAAAGTCAG+TGG - chr4.4:13127744-13127763 None:intergenic 30.0%
TTGATGCTCTTGAAAATCTA+AGG + chr4.4:13127421-13127440 MS.gene023630:CDS 30.0%
! TATTGTTGGAATTCCTGTTT+TGG + chr4.4:13128178-13128197 MS.gene023630:CDS 30.0%
! TCACAAACTGTTGATAAAAC+TGG + chr4.4:13127954-13127973 MS.gene023630:CDS 30.0%
! TGAAAGTGTATGGAATTATC+TGG + chr4.4:13128658-13128677 MS.gene023630:CDS 30.0%
AGATCAACCATAGTAAGATC+AGG - chr4.4:13127777-13127796 None:intergenic 35.0%
ATATCCACACATGTTGAATC+AGG + chr4.4:13128985-13129004 MS.gene023630:CDS 35.0%
ATGGACAGGATTGGAAATTA+TGG + chr4.4:13129274-13129293 MS.gene023630:CDS 35.0%
CAAACAACTTTCAACTGATG+AGG + chr4.4:13127647-13127666 MS.gene023630:CDS 35.0%
CAATCACATCAATTAACCTC+CGG - chr4.4:13129057-13129076 None:intergenic 35.0%
CCTTACTTCAGAGAAAAGTT+AGG + chr4.4:13128606-13128625 MS.gene023630:CDS 35.0%
CTTATTGGAAAATTGTGCTC+CGG + chr4.4:13129035-13129054 MS.gene023630:CDS 35.0%
CTTGAGGAAGCCAAATTTAT+TGG + chr4.4:13128548-13128567 MS.gene023630:CDS 35.0%
CTTTACTATCAAAGTCAGTG+GGG - chr4.4:13127742-13127761 None:intergenic 35.0%
GAATCAGGCTTATGATTTGA+AGG + chr4.4:13129000-13129019 MS.gene023630:CDS 35.0%
GTCTTGGTTATGTTTGTGTA+AGG + chr4.4:13128063-13128082 MS.gene023630:CDS 35.0%
GTTGATCATCAACATCAACA+TGG - chr4.4:13127341-13127360 None:intergenic 35.0%
GTTTGTGTAAGGAATCGAAT+TGG + chr4.4:13128074-13128093 MS.gene023630:CDS 35.0%
TGATACCAGCTAATGATTCA+AGG - chr4.4:13127527-13127546 None:intergenic 35.0%
TGGACAAGGAATTTGTACAA+GGG + chr4.4:13127557-13127576 MS.gene023630:CDS 35.0%
TGGACAGGATTGGAAATTAT+GGG + chr4.4:13129275-13129294 MS.gene023630:CDS 35.0%
TGTGAATGTTGATCTGCAAA+AGG + chr4.4:13129108-13129127 MS.gene023630:CDS 35.0%
TGTGTGGATATTTCATAAGC+TGG - chr4.4:13128976-13128995 None:intergenic 35.0%
TGTTGAAAGTAATGCCACTA+AGG + chr4.4:13128499-13128518 MS.gene023630:CDS 35.0%
TTACTACTTGTGAGTCGATA+AGG + chr4.4:13127928-13127947 MS.gene023630:CDS 35.0%
TTATACTCCAAAACAAGCTC+TGG - chr4.4:13127624-13127643 None:intergenic 35.0%
TTGATCTCCAACAACAACAA+TGG - chr4.4:13127484-13127503 None:intergenic 35.0%
TTTCCAATAAGCTGTCAATC+TGG - chr4.4:13129026-13129045 None:intergenic 35.0%
! ATCAAGTTTGTTTGCCTTTG+TGG - chr4.4:13127295-13127314 None:intergenic 35.0%
! GAACTTTGATTTCCTCCATT+AGG - chr4.4:13128529-13128548 None:intergenic 35.0%
!! AATTTGTACAAGGGTACCTT+TGG + chr4.4:13127566-13127585 MS.gene023630:CDS 35.0%
!!! CCTAACTTTTCTCTGAAGTA+AGG - chr4.4:13128609-13128628 None:intergenic 35.0%
!!! GAGCTTGTTTTGGAGTATAA+TGG + chr4.4:13127624-13127643 MS.gene023630:CDS 35.0%
AAGAGTCTTACGAAGATGCA+AGG + chr4.4:13128099-13128118 MS.gene023630:CDS 40.0%
AAGGCTGATAAATCTCCTGA+AGG + chr4.4:13128002-13128021 MS.gene023630:CDS 40.0%
AAGGTCAGAAAAGCAGTTCT+TGG - chr4.4:13128590-13128609 None:intergenic 40.0%
ACAATTGGTGCAACAGATAG+AGG - chr4.4:13127369-13127388 None:intergenic 40.0%
ACAGCTGACGATGTTAACAT+TGG + chr4.4:13128041-13128060 MS.gene023630:CDS 40.0%
AGGAGTCTCCTTCAATATCA+GGG + chr4.4:13129185-13129204 MS.gene023630:CDS 40.0%
AGTGTCCTTGAATCATTAGC+TGG + chr4.4:13127519-13127538 MS.gene023630:CDS 40.0%
ATAACTCGTGTTCCTGTTCA+TGG + chr4.4:13127804-13127823 MS.gene023630:CDS 40.0%
CACTAAGGATTTCCTAATGG+AGG + chr4.4:13128514-13128533 MS.gene023630:CDS 40.0%
CGTCTCAACATTGCTAAAGA+GGG + chr4.4:13127444-13127463 MS.gene023630:CDS 40.0%
CTCCATTAGGAAATCCTTAG+TGG - chr4.4:13128516-13128535 None:intergenic 40.0%
CTTACTATGGTTGATCTTCC+AGG + chr4.4:13127780-13127799 MS.gene023630:CDS 40.0%
GAACTGTTATCAGCAAATGG+TGG + chr4.4:13129142-13129161 MS.gene023630:CDS 40.0%
GATTTGCAGAAAAGCAGACA+TGG - chr4.4:13128342-13128361 None:intergenic 40.0%
GCAGTTCTTGGCATGAAATT+CGG - chr4.4:13128578-13128597 None:intergenic 40.0%
GGACAGGATTGGAAATTATG+GGG + chr4.4:13129276-13129295 MS.gene023630:CDS 40.0%
GGAGGAAATCAAAGTTCTTG+AGG + chr4.4:13128532-13128551 MS.gene023630:CDS 40.0%
GTGGACAAGGAATTTGTACA+AGG + chr4.4:13127556-13127575 MS.gene023630:CDS 40.0%
GTTGTTGGAGATCAATCATC+TGG + chr4.4:13127489-13127508 MS.gene023630:CDS 40.0%
TAAATCTCCTGAAGGTTTGC+TGG + chr4.4:13128010-13128029 MS.gene023630:CDS 40.0%
TAAGCCTGATTCAACATGTG+TGG - chr4.4:13128992-13129011 None:intergenic 40.0%
TATACTCCTCCAAGTACTCA+GGG - chr4.4:13128850-13128869 None:intergenic 40.0%
TCCAATCCTGTCCATAATGT+TGG - chr4.4:13129269-13129288 None:intergenic 40.0%
TCTCCAACAACAACAATGGT+TGG - chr4.4:13127480-13127499 None:intergenic 40.0%
TGCCACTAAGGATTTCCTAA+TGG + chr4.4:13128511-13128530 MS.gene023630:CDS 40.0%
TGGAAACAAGAGTTCCACAA+AGG + chr4.4:13127278-13127297 MS.gene023630:CDS 40.0%
TGGAGTTCCTGATCTTACTA+TGG + chr4.4:13127767-13127786 MS.gene023630:CDS 40.0%
TGTCTCTTCCCTGATATTGA+AGG - chr4.4:13129196-13129215 None:intergenic 40.0%
TTATACTCCTCCAAGTACTC+AGG - chr4.4:13128851-13128870 None:intergenic 40.0%
! ACAAGCTTTTGAGCCAAAAC+AGG - chr4.4:13128194-13128213 None:intergenic 40.0%
! CTTCCAACCATTGTTGTTGT+TGG + chr4.4:13127474-13127493 MS.gene023630:CDS 40.0%
! GACGATGTTAACATTGGTCT+TGG + chr4.4:13128047-13128066 MS.gene023630:CDS 40.0%
! TCAAGTACTGTGAAGCTGAA+AGG + chr4.4:13128929-13128948 MS.gene023630:CDS 40.0%
! TTTTGCAGCCTCATAACCAA+AGG - chr4.4:13127585-13127604 None:intergenic 40.0%
!! TTATCAGGTTGACCATGAAC+AGG - chr4.4:13127819-13127838 None:intergenic 40.0%
!!! TTTTCAAGAGCATCAAGCAC+TGG - chr4.4:13127417-13127436 None:intergenic 40.0%
ACAGGAACACGAGTTATACC+TGG - chr4.4:13127801-13127820 None:intergenic 45.0%
AGCTTGAAGCACATGCTGAA+TGG - chr4.4:13128792-13128811 None:intergenic 45.0%
AGGCCAGATTGACAGCTTAT+TGG + chr4.4:13129020-13129039 MS.gene023630:CDS 45.0%
ATGTAACCCTGAGTACTTGG+AGG + chr4.4:13128841-13128860 MS.gene023630:CDS 45.0%
ATTGGAAAATTGTGCTCCGG+AGG + chr4.4:13129038-13129057 MS.gene023630:CDS 45.0%
CACATGTAACCCTGAGTACT+TGG + chr4.4:13128838-13128857 MS.gene023630:CDS 45.0%
CTGTTATCAGCAAATGGTGG+TGG + chr4.4:13129145-13129164 MS.gene023630:CDS 45.0%
GAAAGAAAGCAAAGAGACCG+TGG + chr4.4:13129243-13129262 MS.gene023630:CDS 45.0%
GAGGAGTCTCCTTCAATATC+AGG + chr4.4:13129184-13129203 MS.gene023630:CDS 45.0%
GCCAACATTATGGACAGGAT+TGG + chr4.4:13129265-13129284 MS.gene023630:CDS 45.0%
GCCAGTGAGGATAAATTAGC+AGG - chr4.4:13128314-13128333 None:intergenic 45.0%
GCCTGCTAATTTATCCTCAC+TGG + chr4.4:13128310-13128329 MS.gene023630:CDS 45.0%
GCGTCTCAACATTGCTAAAG+AGG + chr4.4:13127443-13127462 MS.gene023630:CDS 45.0%
GTACAAATTCCTTGTCCACG+TGG - chr4.4:13127555-13127574 None:intergenic 45.0%
TATCAAAGTCAGTGGGGTGT+TGG - chr4.4:13127736-13127755 None:intergenic 45.0%
TGTGCTTCAAGCTGTTGAGA+TGG + chr4.4:13128799-13128818 MS.gene023630:CDS 45.0%
! ACTGTGAAGCTGAAAGGTGT+TGG + chr4.4:13128935-13128954 MS.gene023630:CDS 45.0%
! GGAGGAGTATAACAAGCTGA+TGG + chr4.4:13128859-13128878 MS.gene023630:CDS 45.0%
!! CAAGGGTACCTTTGGTTATG+AGG + chr4.4:13127574-13127593 MS.gene023630:CDS 45.0%
ACCTTCTCCAGCAAACCTTC+AGG - chr4.4:13128020-13128039 None:intergenic 50.0%
AGAGACCGTGGCCAACATTA+TGG + chr4.4:13129255-13129274 MS.gene023630:CDS 50.0%
AGTGTCACACACTTCTGTCC+AGG + chr4.4:13128144-13128163 MS.gene023630:CDS 50.0%
CCTGTCCATAATGTTGGCCA+CGG - chr4.4:13129263-13129282 None:intergenic 50.0%
GCTCTGGCTGTGGAAGTAAA+TGG - chr4.4:13127608-13127627 None:intergenic 50.0%
GGAAGAGACAGAAGTTGAGC+AGG + chr4.4:13129206-13129225 MS.gene023630:CDS 50.0%
GGTGCAACAGATAGAGGTTG+AGG - chr4.4:13127363-13127382 None:intergenic 50.0%
TGCTATAAACGCAGCCACTG+AGG + chr4.4:13127683-13127702 MS.gene023630:CDS 50.0%
! GGCTACTAGTTTGCATGAGC+TGG + chr4.4:13128280-13128299 MS.gene023630:CDS 50.0%
! TCCAAAACAAGCTCTGGCTG+TGG - chr4.4:13127618-13127637 None:intergenic 50.0%
!! AACGCTTGCTGTCGTGACAA+AGG + chr4.4:13127983-13128002 MS.gene023630:CDS 50.0%
!! CAAATGGTGGTGGTGTTGAG+AGG + chr4.4:13129155-13129174 MS.gene023630:CDS 50.0%
!! TCCACAGCCAGAGCTTGTTT+TGG + chr4.4:13127614-13127633 MS.gene023630:CDS 50.0%
!! TCCTGAAGGTTTGCTGGAGA+AGG + chr4.4:13128016-13128035 MS.gene023630:CDS 50.0%
!! TGGGGTGTTGGAAATCCCTT+TGG - chr4.4:13127724-13127743 None:intergenic 50.0%
AGACATGGCATCAGCCAGTG+AGG - chr4.4:13128327-13128346 None:intergenic 55.0%
ATCAGCTTGCCACGTGGACA+AGG + chr4.4:13127543-13127562 MS.gene023630:CDS 55.0%
CCGTGGCCAACATTATGGAC+AGG + chr4.4:13129260-13129279 MS.gene023630:CDS 55.0%
! TGGTGTTGAGAGGCTGCTTG+AGG + chr4.4:13129165-13129184 MS.gene023630:CDS 55.0%
!! AGCTTGCTGGCACTGCCAAA+GGG + chr4.4:13127706-13127725 MS.gene023630:CDS 55.0%
AGTGCCAGCAAGCTCCTCAG+TGG - chr4.4:13127700-13127719 None:intergenic 60.0%
GCTGGTATCAGCTTGCCACG+TGG + chr4.4:13127537-13127556 MS.gene023630:CDS 60.0%
!! GAGCTTGCTGGCACTGCCAA+AGG + chr4.4:13127705-13127724 MS.gene023630:CDS 60.0%
GCAGCCACTGAGGAGCTTGC+TGG + chr4.4:13127693-13127712 MS.gene023630:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 13127270 13129306 13127270 ID=MS.gene023630
chr4.4 mRNA 13127270 13129306 13127270 ID=MS.gene023630.t1;Parent=MS.gene023630
chr4.4 exon 13127270 13129306 13127270 ID=MS.gene023630.t1.exon1;Parent=MS.gene023630.t1
chr4.4 CDS 13127270 13129306 13127270 ID=cds.MS.gene023630.t1;Parent=MS.gene023630.t1
Gene Sequence

>MS.gene023630

ATGACAATTGGAAACAAGAGTTCCACAAAGGCAAACAAACTTGATGAATCATCATCACTTGCATTAGTCCATGTTGATGTTGATGATCAACCTCAACCTCTATCTGTTGCACCAATTGTATCTTCTTATAATGAGAAGATACGTCCAGTGCTTGATGCTCTTGAAAATCTAAGGCGTCTCAACATTGCTAAAGAGGGAATTCAGCTTCCAACCATTGTTGTTGTTGGAGATCAATCATCTGGAAAGTCTAGTGTCCTTGAATCATTAGCTGGTATCAGCTTGCCACGTGGACAAGGAATTTGTACAAGGGTACCTTTGGTTATGAGGCTGCAAAACCATTTACTTCCACAGCCAGAGCTTGTTTTGGAGTATAATGGCAAACAACTTTCAACTGATGAGGAAAATGTATCTGATGCTATAAACGCAGCCACTGAGGAGCTTGCTGGCACTGCCAAAGGGATTTCCAACACCCCACTGACTTTGATAGTAAAGAAGAATGGAGTTCCTGATCTTACTATGGTTGATCTTCCAGGTATAACTCGTGTTCCTGTTCATGGTCAACCTGATAACATTTATGATCAGATTAAGGATATTATCATGGAGTATATAACTCCTGAAGAGAGTATTATTTTGAATGTGCTCTCTGCTACTGTTGATTTTACTACTTGTGAGTCGATAAGGATGTCACAAACTGTTGATAAAACTGGTTTGAGAACGCTTGCTGTCGTGACAAAGGCTGATAAATCTCCTGAAGGTTTGCTGGAGAAGGTTACAGCTGACGATGTTAACATTGGTCTTGGTTATGTTTGTGTAAGGAATCGAATTGGTGAAGAGTCTTACGAAGATGCAAGGAATGAAGAAGAGAAGCTGTTCGAGTGTCACACACTTCTGTCCAGGATAGACAAATCTATTGTTGGAATTCCTGTTTTGGCTCAAAAGCTTGTTCACGTTCAAGCGATGATTATATCTAAAACTTTGCCAGAGATCATTAAGAAGATTAATGAGAAACTGGCTACTAGTTTGCATGAGCTGGAAACTTTGCCTGCTAATTTATCCTCACTGGCTGATGCCATGTCTGCTTTTCTGCAAATCATTTCTTTGTCAAGAGACTCTCTGAAAAAAATTCTTCTTATAGGAGAGTTTGAAGAGTATTCTGATGAAAAGAAAATGCATTGCACGGCTCGATTAGTAGATATGCTGAATTCATACGCAAGTGAGCTTCAAAACTGTGTTGAAAGTAATGCCACTAAGGATTTCCTAATGGAGGAAATCAAAGTTCTTGAGGAAGCCAAATTTATTGGTCTTCCGAATTTCATGCCAAGAACTGCTTTTCTGACCTTACTTCAGAGAAAAGTTAGGGGCATTTCTTATATGCCAATTAATTTTGTTGAAAGTGTATGGAATTATCTGGAAACTGTTGTTGTTTCAGTTTTGAATCGTCATTCTTCTAACTATTATCAGCTTCAGGTTTCAATTCGTAGAGCTGCTGAGCACCTTATTGCTAAGAAGAAAAAGAATTCCATTCAGCATGTGCTTCAAGCTGTTGAGATGGAGAAGCTTACAGACTACACATGTAACCCTGAGTACTTGGAGGAGTATAACAAGCTGATGGCTCATCAAGAAGTGTTTTTGAATGAAGTTTTGAATGTTAAACGAGCTTCAAGTACTGTGAAGCTGAAAGGTGTTGGTGACGTTGATGTTGTCCAGCTTATGAAATATCCACACATGTTGAATCAGGCTTATGATTTGAAGGCCAGATTGACAGCTTATTGGAAAATTGTGCTCCGGAGGTTAATTGATGTGATTGCTTTGCATTTGATGTTAAGCATCAATGAACTTGTGAATGTTGATCTGCAAAAGGAGATCTGTAATGAACTGTTATCAGCAAATGGTGGTGGTGTTGAGAGGCTGCTTGAGGAGTCTCCTTCAATATCAGGGAAGAGACAGAAGTTGAGCAGGAGTGTGACAGTTTTGAAAGAAAGCAAAGAGACCGTGGCCAACATTATGGACAGGATTGGAAATTATGGGGATAACTAG
Protein sequence

>MS.gene023630.t1

MTIGNKSSTKANKLDESSSLALVHVDVDDQPQPLSVAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLVMRLQNHLLPQPELVLEYNGKQLSTDEENVSDAINAATEELAGTAKGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQTVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGEESYEDARNEEEKLFECHTLLSRIDKSIVGIPVLAQKLVHVQAMIISKTLPEIIKKINEKLATSLHELETLPANLSSLADAMSAFLQIISLSRDSLKKILLIGEFEEYSDEKKMHCTARLVDMLNSYASELQNCVESNATKDFLMEEIKVLEEAKFIGLPNFMPRTAFLTLLQRKVRGISYMPINFVESVWNYLETVVVSVLNRHSSNYYQLQVSIRRAAEHLIAKKKKNSIQHVLQAVEMEKLTDYTCNPEYLEEYNKLMAHQEVFLNEVLNVKRASSTVKLKGVGDVDVVQLMKYPHMLNQAYDLKARLTAYWKIVLRRLIDVIALHLMLSINELVNVDLQKEICNELLSANGGGVERLLEESPSISGKRQKLSRSVTVLKESKETVANIMDRIGNYGDN