Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024160.t1 | XP_013449418.1 | 93.3 | 253 | 4 | 1 | 1 | 240 | 1 | 253 | 2.30E-122 | 448.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024160.t1 | Q94JU3 | 71.3 | 254 | 58 | 3 | 1 | 240 | 1 | 253 | 3.3e-93 | 342.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024160.t1 | A0A072U221 | 93.3 | 253 | 4 | 1 | 1 | 240 | 1 | 253 | 1.7e-122 | 448.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene024160 | MS.gene013885 | PPI |
| MS.gene41848 | MS.gene024160 | PPI |
| MS.gene024160 | MS.gene042550 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024160.t1 | MTR_7g081735 | 93.281 | 253 | 4 | 1 | 1 | 240 | 1 | 253 | 3.79e-172 | 475 |
| MS.gene024160.t1 | MTR_7g081735 | 92.377 | 223 | 4 | 1 | 1 | 210 | 1 | 223 | 4.37e-147 | 411 |
| MS.gene024160.t1 | MTR_7g081735 | 92.377 | 223 | 4 | 1 | 1 | 210 | 1 | 223 | 5.17e-147 | 411 |
| MS.gene024160.t1 | MTR_7g081735 | 91.964 | 224 | 5 | 1 | 1 | 211 | 1 | 224 | 3.91e-146 | 409 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024160.t1 | AT1G02090 | 71.260 | 254 | 58 | 3 | 1 | 240 | 1 | 253 | 1.33e-128 | 365 |
| MS.gene024160.t1 | AT1G02090 | 73.303 | 221 | 45 | 2 | 1 | 208 | 1 | 220 | 5.78e-115 | 329 |
| MS.gene024160.t1 | AT1G02090 | 73.303 | 221 | 45 | 2 | 1 | 208 | 1 | 220 | 1.36e-114 | 328 |
Find 62 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTCTCCCCAATGTTCTTC+AGG | 0.215091 | 7.1:-22695167 | MS.gene024160:intron |
| GCCTGTTTGCACATGGAATA+TGG | 0.229485 | 7.1:-22694651 | MS.gene024160:CDS |
| CAATTCAAGACAAGATAAAA+TGG | 0.274906 | 7.1:-22690015 | MS.gene024160:CDS |
| ACAAGACGGTCCTGCTGTTC+AGG | 0.355508 | 7.1:+22687367 | None:intergenic |
| GAGGAGATCTGCGCTGAATC+TGG | 0.390426 | 7.1:-22689129 | MS.gene024160:CDS |
| CCTTCTCTTCTACCTCCTTC+CGG | 0.397288 | 7.1:+22689960 | None:intergenic |
| CTCCTGCATCAATTGGTCAT+AGG | 0.398251 | 7.1:+22692909 | None:intergenic |
| TGCTGATCGCCTTCCACAAT+TGG | 0.401479 | 7.1:-22693344 | MS.gene024160:CDS |
| TCCATATTCCATGTGCAAAC+AGG | 0.434794 | 7.1:+22694650 | None:intergenic |
| GACTTGAGGCCTGGTCAACT+TGG | 0.435726 | 7.1:-22690447 | MS.gene024160:CDS |
| CATCTAGCTCCTGCATCAAT+TGG | 0.441053 | 7.1:+22692902 | None:intergenic |
| GTTTCTAGTGCCTGAACAGC+AGG | 0.453926 | 7.1:-22687377 | MS.gene024160:CDS |
| ACTGATAGGAAATACCTTGT+AGG | 0.465600 | 7.1:+22693267 | None:intergenic |
| CGCTGAATCTGGTGGAGTGA+TGG | 0.471741 | 7.1:-22689118 | MS.gene024160:CDS |
| TCTTACCAGGGAATAGTAAG+AGG | 0.471798 | 7.1:-22690644 | MS.gene024160:intron |
| GAACAGCAGGACCGTCTTGT+TGG | 0.472927 | 7.1:-22687364 | MS.gene024160:CDS |
| TCCTGCATCAATTGGTCATA+GGG | 0.479189 | 7.1:+22692910 | None:intergenic |
| AATTCAAGACAAGATAAAAT+GGG | 0.481752 | 7.1:-22690014 | MS.gene024160:CDS |
| GGGAATAGTAAGAGGGAAGC+TGG | 0.491836 | 7.1:-22690636 | MS.gene024160:CDS |
| CAGAGAAAGCGAAGAGCAAA+GGG | 0.492940 | 7.1:+22695197 | None:intergenic |
| GGAAGAGATGGCTGAAGCAT+TGG | 0.495343 | 7.1:+22695243 | None:intergenic |
| CGCAGATCTCCTCATGCCCT+CGG | 0.498832 | 7.1:+22689139 | None:intergenic |
| CTGTAAGTTGCTTAAGCTTG+AGG | 0.515959 | 7.1:+22693308 | None:intergenic |
| AATCCAACCGACCTTGAAGA+TGG | 0.517129 | 7.1:+22689919 | None:intergenic |
| ATCATGTTCCCAAGTTGACC+AGG | 0.520527 | 7.1:+22690438 | None:intergenic |
| GCAGGTAGGGACTTGAGGCC+TGG | 0.520956 | 7.1:-22690456 | MS.gene024160:intron |
| TTAAGCTTGAGGATCTGATC+AGG | 0.521602 | 7.1:+22693319 | None:intergenic |
| TCTCAAATCACCTCTTTGGT+CGG | 0.521918 | 7.1:+22689076 | None:intergenic |
| TCAGAGAAAGCGAAGAGCAA+AGG | 0.523965 | 7.1:+22695196 | None:intergenic |
| CTTGTTGGTGACATGGGATG+AGG | 0.527155 | 7.1:-22687349 | None:intergenic |
| TTGTCTCTGTTCAATGTCCA+TGG | 0.528524 | 7.1:+22695309 | None:intergenic |
| AGTGAAGAAGTCCATCTTCA+AGG | 0.529066 | 7.1:-22689930 | MS.gene024160:intron |
| ATCTGATCAGGTACCAATTG+TGG | 0.530315 | 7.1:+22693331 | None:intergenic |
| TTGCAGCAGGTAGGGACTTG+AGG | 0.540523 | 7.1:-22690461 | MS.gene024160:intron |
| GGACCGTCTTGTTGGTGACA+TGG | 0.541332 | 7.1:-22687356 | MS.gene024160:CDS |
| GGACATTGAACAGAGACAAT+CGG | 0.551034 | 7.1:-22695305 | MS.gene024160:CDS |
| CCGGAAGGAGGTAGAAGAGA+AGG | 0.558953 | 7.1:-22689960 | MS.gene024160:CDS |
| CAAACAGGCGAAGCATATCA+AGG | 0.564528 | 7.1:+22694665 | None:intergenic |
| ATGCTTCGCCTGTTTGCACA+TGG | 0.570097 | 7.1:-22694658 | MS.gene024160:CDS |
| TGAAGCATTGGAAGCAGCAG+AGG | 0.576714 | 7.1:+22695255 | None:intergenic |
| ACCCTATGACCAATTGATGC+AGG | 0.577127 | 7.1:-22692911 | MS.gene024160:CDS |
| CATCGACTTCCGAGGGCATG+AGG | 0.581510 | 7.1:-22689148 | MS.gene024160:CDS |
| GACCGTCTTGTTGGTGACAT+GGG | 0.585123 | 7.1:-22687355 | MS.gene024160:CDS |
| ACTTGAGGCCTGGTCAACTT+GGG | 0.588224 | 7.1:-22690446 | MS.gene024160:CDS |
| ATCGACAATGACGGAAGAGA+TGG | 0.590251 | 7.1:+22695231 | None:intergenic |
| GACACTCGCTGAGACCTACA+AGG | 0.594089 | 7.1:-22693281 | MS.gene024160:intron |
| GAGATCTGCGCTGAATCTGG+TGG | 0.603185 | 7.1:-22689126 | MS.gene024160:CDS |
| CAGGCCGACATCGACTTCCG+AGG | 0.604650 | 7.1:-22689156 | MS.gene024160:CDS |
| GTGAAGTTGACAAGAAGCAC+CGG | 0.618024 | 7.1:-22689979 | MS.gene024160:CDS |
| ATGCCCTCGGAAGTCGATGT+CGG | 0.619533 | 7.1:+22689152 | None:intergenic |
| TGACAAGAAGCACCGGAAGG+AGG | 0.619732 | 7.1:-22689972 | MS.gene024160:CDS |
| AGAAGTAGCATCGACAATGA+CGG | 0.621413 | 7.1:+22695222 | None:intergenic |
| TGATACAGACACTCTCCGAC+TGG | 0.626737 | 7.1:-22690419 | MS.gene024160:intron |
| AGGCCGACATCGACTTCCGA+GGG | 0.629042 | 7.1:-22689155 | MS.gene024160:CDS |
| CTTACCAGGGAATAGTAAGA+GGG | 0.637542 | 7.1:-22690643 | MS.gene024160:intron |
| AAGATCGAAGCCGACCAAAG+AGG | 0.643099 | 7.1:-22689086 | MS.gene024160:intron |
| GATCAGGTACCAATTGTGGA+AGG | 0.648153 | 7.1:+22693335 | None:intergenic |
| ATCCCATGTCACCAACAAGA+CGG | 0.649033 | 7.1:+22687353 | None:intergenic |
| TTGTTGGTGACATGGGATGA+GGG | 0.651568 | 7.1:-22687348 | None:intergenic |
| AGTTGACAAGAAGCACCGGA+AGG | 0.685488 | 7.1:-22689975 | MS.gene024160:CDS |
| GATTAATGAGTGCATGTATG+CGG | 0.729577 | 7.1:-22692848 | MS.gene024160:intron |
| GGGGAACCTGAAGAACATTG+GGG | 0.769091 | 7.1:+22695161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 22687351 | 22695329 | 22687351 | ID=MS.gene024160 |
| chr7.1 | mRNA | 22687351 | 22695329 | 22687351 | ID=MS.gene024160.t1;Parent=MS.gene024160 |
| chr7.1 | exon | 22695168 | 22695329 | 22695168 | ID=MS.gene024160.t1.exon1;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22695168 | 22695329 | 22695168 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22694638 | 22694713 | 22694638 | ID=MS.gene024160.t1.exon2;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22694638 | 22694713 | 22694638 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22693282 | 22693370 | 22693282 | ID=MS.gene024160.t1.exon3;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22693282 | 22693370 | 22693282 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22692849 | 22692938 | 22692849 | ID=MS.gene024160.t1.exon4;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22692849 | 22692938 | 22692849 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22690632 | 22690657 | 22690632 | ID=MS.gene024160.t1.exon5;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22690632 | 22690657 | 22690632 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22690420 | 22690470 | 22690420 | ID=MS.gene024160.t1.exon6;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22690420 | 22690470 | 22690420 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22689931 | 22690066 | 22689931 | ID=MS.gene024160.t1.exon7;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22689931 | 22690066 | 22689931 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22689087 | 22689175 | 22689087 | ID=MS.gene024160.t1.exon8;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22689087 | 22689175 | 22689087 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| chr7.1 | exon | 22687351 | 22687402 | 22687351 | ID=MS.gene024160.t1.exon9;Parent=MS.gene024160.t1 |
| chr7.1 | CDS | 22687351 | 22687402 | 22687351 | ID=cds.MS.gene024160.t1;Parent=MS.gene024160.t1 |
| Gene Sequence |
| Protein sequence |
