Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024226.t1 | XP_003624429.1 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 8.80E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024226.t1 | B9RH17 | 59.4 | 69 | 28 | 0 | 115 | 183 | 119 | 187 | 2.4e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024226.t1 | G7KW47 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 6.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene024226 | MS.gene63947 | PPI |
| MS.gene024226 | MS.gene025030 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024226.t1 | MTR_7g083120 | 96.774 | 186 | 6 | 0 | 1 | 186 | 1 | 186 | 1.45e-133 | 371 |
| MS.gene024226.t1 | MTR_7g083120 | 95.726 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 3.03e-76 | 227 |
| MS.gene024226.t1 | MTR_4g118810 | 29.655 | 145 | 88 | 3 | 33 | 166 | 36 | 177 | 5.29e-16 | 72.8 |
| MS.gene024226.t1 | MTR_0004s0210 | 29.927 | 137 | 88 | 1 | 33 | 169 | 11 | 139 | 1.59e-15 | 70.9 |
| MS.gene024226.t1 | MTR_4g081880 | 32.099 | 162 | 105 | 3 | 11 | 169 | 10 | 169 | 1.85e-14 | 68.6 |
| MS.gene024226.t1 | MTR_7g108130 | 30.496 | 141 | 92 | 3 | 33 | 169 | 32 | 170 | 6.94e-14 | 67.0 |
| MS.gene024226.t1 | MTR_4g118800 | 36.000 | 125 | 79 | 1 | 33 | 157 | 31 | 154 | 9.21e-13 | 63.9 |
| MS.gene024226.t1 | MTR_2g020060 | 27.778 | 162 | 104 | 2 | 33 | 184 | 26 | 184 | 2.74e-12 | 62.8 |
| MS.gene024226.t1 | MTR_7g011090 | 30.435 | 161 | 107 | 3 | 11 | 169 | 11 | 168 | 1.23e-11 | 60.8 |
| MS.gene024226.t1 | MTR_5g076480 | 24.242 | 165 | 118 | 3 | 22 | 186 | 29 | 186 | 3.80e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024226.t1 | AT4G25040 | 36.424 | 151 | 92 | 1 | 31 | 181 | 18 | 164 | 3.48e-21 | 85.9 |
| MS.gene024226.t1 | AT4G03540 | 34.959 | 123 | 72 | 1 | 47 | 169 | 26 | 140 | 6.31e-18 | 77.0 |
| MS.gene024226.t1 | AT4G15630 | 26.984 | 189 | 119 | 4 | 6 | 184 | 10 | 189 | 1.12e-15 | 72.0 |
| MS.gene024226.t1 | AT4G15620 | 30.435 | 161 | 100 | 3 | 34 | 184 | 31 | 189 | 3.41e-15 | 70.5 |
| MS.gene024226.t1 | AT1G03700 | 32.143 | 112 | 68 | 1 | 45 | 156 | 24 | 127 | 3.23e-14 | 67.4 |
| MS.gene024226.t1 | AT4G15610 | 28.105 | 153 | 105 | 2 | 10 | 157 | 9 | 161 | 4.20e-12 | 62.4 |
| MS.gene024226.t1 | AT5G15290 | 30.216 | 139 | 90 | 3 | 33 | 167 | 31 | 166 | 7.69e-12 | 61.6 |
| MS.gene024226.t1 | AT3G06390 | 25.455 | 165 | 108 | 5 | 5 | 157 | 9 | 170 | 4.19e-11 | 59.7 |
Find 30 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGCCTTCTTTGCCAATT+TGG | 0.186565 | 7.1:+21928630 | MS.gene024226:CDS |
| TTGGCCTTCTTTGCCAATTT+GGG | 0.251825 | 7.1:+21928631 | MS.gene024226:CDS |
| AAAGTTCTGCAAAACCAATT+TGG | 0.258540 | 7.1:+21928588 | MS.gene024226:CDS |
| TAAAATTGTGAGTCCCAAAT+TGG | 0.269455 | 7.1:-21928644 | None:intergenic |
| TTCCATCCTGAAGGAATCTT+TGG | 0.318409 | 7.1:-21927211 | None:intergenic |
| AAGCAAAAGTGAAACCAAAT+TGG | 0.326060 | 7.1:-21928602 | None:intergenic |
| GGATGAGGTTGTTGAAGATT+TGG | 0.339479 | 7.1:-21927235 | None:intergenic |
| ACATCATAGGACTTTCTTCA+TGG | 0.382860 | 7.1:+21927266 | MS.gene024226:CDS |
| CTGTTATGCTCTTCTCTATC+CGG | 0.383937 | 7.1:+21927364 | MS.gene024226:CDS |
| GATGAGGTTGTTGAAGATTT+GGG | 0.392076 | 7.1:-21927234 | None:intergenic |
| TAATTAAGAGGTGAACTTGT+AGG | 0.396788 | 7.1:-21928671 | None:intergenic |
| GTTGGATACGTGGGACAGTA+TGG | 0.412618 | 7.1:+21928523 | MS.gene024226:CDS |
| CTGATGCCAAAGATTCCTTC+AGG | 0.434784 | 7.1:+21927205 | MS.gene024226:CDS |
| TCAAAACATCCTCAGAATCT+TGG | 0.440400 | 7.1:+21927290 | MS.gene024226:CDS |
| GAGAGGAGAAAATAATTAAG+AGG | 0.459921 | 7.1:-21928683 | None:intergenic |
| ATGACGGTGTTGCTGATAGC+TGG | 0.468432 | 7.1:+21928481 | MS.gene024226:CDS |
| AATAAAAGTGAGCTTCAAAC+CGG | 0.511834 | 7.1:-21927383 | None:intergenic |
| ATGGAGAGAAACACGTTGGA+TGG | 0.514689 | 7.1:+21928542 | MS.gene024226:CDS |
| GAGCATAACAGATTGGTTGT+TGG | 0.526003 | 7.1:-21927352 | None:intergenic |
| GAGTCCCAAATTGGCAAAGA+AGG | 0.541836 | 7.1:-21928635 | None:intergenic |
| TAGAGAAGAGCATAACAGAT+TGG | 0.552990 | 7.1:-21927359 | None:intergenic |
| AGATTTGGGTTCCATCCTGA+AGG | 0.555523 | 7.1:-21927220 | None:intergenic |
| AGCCACAGCCGTTGGATACG+TGG | 0.562855 | 7.1:+21928513 | MS.gene024226:CDS |
| TGTTCAGCAGCCACAGCCGT+TGG | 0.589627 | 7.1:+21928505 | MS.gene024226:CDS |
| TCCCACGTATCCAACGGCTG+TGG | 0.593429 | 7.1:-21928515 | None:intergenic |
| GCCACAGCCGTTGGATACGT+GGG | 0.599202 | 7.1:+21928514 | MS.gene024226:CDS |
| TGCCAAAGATTCCTTCAGGA+TGG | 0.603471 | 7.1:+21927209 | MS.gene024226:CDS |
| GAACAATGACCAAGATTCTG+AGG | 0.616784 | 7.1:-21927299 | None:intergenic |
| ATACTGTCCCACGTATCCAA+CGG | 0.624247 | 7.1:-21928521 | None:intergenic |
| CAGTATGGAGAGAAACACGT+TGG | 0.708078 | 7.1:+21928538 | MS.gene024226:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGACATTATATTTTTTAT+AGG | + | chr7.1:21928119-21928138 | MS.gene024226:intron | 10.0% |
| !!! | ATGACATTATATTTTTTATA+GGG | + | chr7.1:21928120-21928139 | MS.gene024226:intron | 10.0% |
| !! | TCATATGTATAAACAAAAAA+GGG | + | chr7.1:21927458-21927477 | MS.gene024226:intron | 15.0% |
| !! | TTCAATATAATTATACATGT+CGG | - | chr7.1:21928254-21928273 | None:intergenic | 15.0% |
| !! | TTCATATGTATAAACAAAAA+AGG | + | chr7.1:21927457-21927476 | MS.gene024226:intron | 15.0% |
| !! | TTTGTTTATACATATGAAAA+TGG | - | chr7.1:21927455-21927474 | None:intergenic | 15.0% |
| !!! | TGACATTATATTTTTTATAG+GGG | + | chr7.1:21928121-21928140 | MS.gene024226:intron | 15.0% |
| !!! | TGATTTTCTTAAATTATTAG+CGG | + | chr7.1:21928279-21928298 | MS.gene024226:intron | 15.0% |
| !! | AAACAATGACATGTATAATT+TGG | - | chr7.1:21928067-21928086 | None:intergenic | 20.0% |
| !! | ACTTGACTAAAACATTAAAT+GGG | - | chr7.1:21928011-21928030 | None:intergenic | 20.0% |
| !! | ATACATATGAAAATGGAAAA+TGG | - | chr7.1:21927448-21927467 | None:intergenic | 20.0% |
| !! | TACATATGAAAATGGAAAAT+GGG | - | chr7.1:21927447-21927466 | None:intergenic | 20.0% |
| !!! | CTATTATTTCTTCCTATTTT+TGG | + | chr7.1:21927637-21927656 | MS.gene024226:CDS | 20.0% |
| !!! | TCTGTATTTATTTTTGTCTT+TGG | + | chr7.1:21927512-21927531 | MS.gene024226:intron | 20.0% |
| ! | ATACATGTCATTGTTTATCA+TGG | + | chr7.1:21928071-21928090 | MS.gene024226:intron | 25.0% |
| ! | GACTTGACTAAAACATTAAA+TGG | - | chr7.1:21928012-21928031 | None:intergenic | 25.0% |
| ! | TCTTTCTAATCCACAAAATT+AGG | + | chr7.1:21928389-21928408 | MS.gene024226:intron | 25.0% |
| ! | TTACCAAATCAACCAAAAAT+AGG | - | chr7.1:21927652-21927671 | None:intergenic | 25.0% |
| ! | TTAGAAAGAAAGGTAAATGA+TGG | - | chr7.1:21928378-21928397 | None:intergenic | 25.0% |
| !! | AAATAATAGTCTTTTCGTTG+AGG | - | chr7.1:21927626-21927645 | None:intergenic | 25.0% |
| !! | GGAATATGATTGATAATATG+TGG | + | chr7.1:21928092-21928111 | MS.gene024226:intron | 25.0% |
| !! | TGATAATCAATCTTTTGATG+AGG | + | chr7.1:21927591-21927610 | MS.gene024226:CDS | 25.0% |
| !!! | AAAATTGGTGAATTTTGATC+AGG | + | chr7.1:21928456-21928475 | MS.gene024226:intron | 25.0% |
| !!! | AAGCCAATAACATTTTAACA+TGG | - | chr7.1:21927746-21927765 | None:intergenic | 25.0% |
| !!! | ATTTTGTGGATTAGAAAGAA+AGG | - | chr7.1:21928388-21928407 | None:intergenic | 25.0% |
| !!! | CATGTTTGTTATGAGAAAAT+TGG | + | chr7.1:21928441-21928460 | MS.gene024226:intron | 25.0% |
| !!! | TTCACTTTTGCTTTCATATT+TGG | + | chr7.1:21928612-21928631 | MS.gene024226:CDS | 25.0% |
| AAAGTTCTGCAAAACCAATT+TGG | + | chr7.1:21928588-21928607 | MS.gene024226:CDS | 30.0% | |
| AAGCAAAAGTGAAACCAAAT+TGG | - | chr7.1:21928605-21928624 | None:intergenic | 30.0% | |
| AATAAAAGTGAGCTTCAAAC+CGG | - | chr7.1:21927386-21927405 | None:intergenic | 30.0% | |
| GACAGTATTTATGTTATGTC+TGG | + | chr7.1:21928311-21928330 | MS.gene024226:intron | 30.0% | |
| GGTCCATGTTAAAATGTTAT+TGG | + | chr7.1:21927740-21927759 | MS.gene024226:intron | 30.0% | |
| TAAAATTGTGAGTCCCAAAT+TGG | - | chr7.1:21928647-21928666 | None:intergenic | 30.0% | |
| TCTTAAATTATTAGCGGTGT+CGG | + | chr7.1:21928285-21928304 | MS.gene024226:intron | 30.0% | |
| TTACTAGTGTCAATTGTAGT+GGG | + | chr7.1:21927924-21927943 | MS.gene024226:intron | 30.0% | |
| !!! | AGATTTTTTGTTGCTGCAAA+TGG | + | chr7.1:21927539-21927558 | MS.gene024226:intron | 30.0% |
| !!! | CTTCCTATTTTTGGTTGATT+TGG | + | chr7.1:21927646-21927665 | MS.gene024226:CDS | 30.0% |
| ACATCATAGGACTTTCTTCA+TGG | + | chr7.1:21927266-21927285 | MS.gene024226:CDS | 35.0% | |
| CATCCTCACAAAAACATCAT+AGG | + | chr7.1:21927253-21927272 | MS.gene024226:CDS | 35.0% | |
| CTTACTAGTGTCAATTGTAG+TGG | + | chr7.1:21927923-21927942 | MS.gene024226:intron | 35.0% | |
| GATGAGGTTGTTGAAGATTT+GGG | - | chr7.1:21927237-21927256 | None:intergenic | 35.0% | |
| TATCCGTATTCATGCTTCAT+AGG | + | chr7.1:21928340-21928359 | MS.gene024226:intron | 35.0% | |
| TCAAAACATCCTCAGAATCT+TGG | + | chr7.1:21927290-21927309 | MS.gene024226:CDS | 35.0% | |
| TGACCTATGAAGCATGAATA+CGG | - | chr7.1:21928346-21928365 | None:intergenic | 35.0% | |
| TGTCTGAAAACAGAAATTGC+AGG | + | chr7.1:21927719-21927738 | MS.gene024226:intron | 35.0% | |
| ! | CTTATTCGCTCCTAATTTTG+TGG | - | chr7.1:21928402-21928421 | None:intergenic | 35.0% |
| ! | TAGAGAAGAGCATAACAGAT+TGG | - | chr7.1:21927362-21927381 | None:intergenic | 35.0% |
| ! | TTCACAGATGTCTTTTACTC+TGG | + | chr7.1:21928223-21928242 | MS.gene024226:intron | 35.0% |
| ! | TTGGCTTAGACATGTTTTGT+CGG | + | chr7.1:21927759-21927778 | MS.gene024226:intron | 35.0% |
| ! | TTTTGCAGAACTTTTGCACA+TGG | - | chr7.1:21928582-21928601 | None:intergenic | 35.0% |
| !! | AGTCCTATGATGTTTTTGTG+AGG | - | chr7.1:21927259-21927278 | None:intergenic | 35.0% |
| !! | ATGATGTTTTTGTGAGGATG+AGG | - | chr7.1:21927253-21927272 | None:intergenic | 35.0% |
| !! | GAATTTTGATCAGGTGATGA+CGG | + | chr7.1:21928465-21928484 | MS.gene024226:intron | 35.0% |
| !! | TGAAAATGGAAAATGGGTTG+TGG | - | chr7.1:21927441-21927460 | None:intergenic | 35.0% |
| !!! | AGTAGTTTTGAGTTAGACCT+TGG | + | chr7.1:21927868-21927887 | MS.gene024226:intron | 35.0% |
| ATTGAAGTCGAGACTTTGAG+AGG | - | chr7.1:21927902-21927921 | None:intergenic | 40.0% | |
| CTGTTATGCTCTTCTCTATC+CGG | + | chr7.1:21927364-21927383 | MS.gene024226:CDS | 40.0% | |
| GAACAATGACCAAGATTCTG+AGG | - | chr7.1:21927302-21927321 | None:intergenic | 40.0% | |
| GAGTTGTAACCTACTTAGGT+TGG | + | chr7.1:21927837-21927856 | MS.gene024226:intron | 40.0% | |
| GGATGAGGTTGTTGAAGATT+TGG | - | chr7.1:21927238-21927257 | None:intergenic | 40.0% | |
| TCAACCAAACCAACCTAAGT+AGG | - | chr7.1:21927849-21927868 | None:intergenic | 40.0% | |
| TGGTTAATTACCTCAGCACT+AGG | - | chr7.1:21928047-21928066 | None:intergenic | 40.0% | |
| TTCCATCCTGAAGGAATCTT+TGG | - | chr7.1:21927214-21927233 | None:intergenic | 40.0% | |
| ! | GAGCATAACAGATTGGTTGT+TGG | - | chr7.1:21927355-21927374 | None:intergenic | 40.0% |
| ! | TTGGCCTTCTTTGCCAATTT+GGG | + | chr7.1:21928631-21928650 | MS.gene024226:CDS | 40.0% |
| ! | TTTGAGAGGAGTATACTCCA+AGG | - | chr7.1:21927888-21927907 | None:intergenic | 40.0% |
| ! | TTTGGCCTTCTTTGCCAATT+TGG | + | chr7.1:21928630-21928649 | MS.gene024226:CDS | 40.0% |
| ! | TTTTGTTGCTGCAAATGGTG+TGG | + | chr7.1:21927544-21927563 | MS.gene024226:CDS | 40.0% |
| !! | GTAACCTACTTAGGTTGGTT+TGG | + | chr7.1:21927842-21927861 | MS.gene024226:intron | 40.0% |
| !! | GTCAATTGTAGTGGGTTAGT+CGG | + | chr7.1:21927932-21927951 | MS.gene024226:intron | 40.0% |
| ATACTGTCCCACGTATCCAA+CGG | - | chr7.1:21928524-21928543 | None:intergenic | 45.0% | |
| ATGGAGAGAAACACGTTGGA+TGG | + | chr7.1:21928542-21928561 | MS.gene024226:CDS | 45.0% | |
| CAGTATGGAGAGAAACACGT+TGG | + | chr7.1:21928538-21928557 | MS.gene024226:CDS | 45.0% | |
| CGCAGAGTTGTAACCTACTT+AGG | + | chr7.1:21927833-21927852 | MS.gene024226:intron | 45.0% | |
| CTGATGCCAAAGATTCCTTC+AGG | + | chr7.1:21927205-21927224 | MS.gene024226:CDS | 45.0% | |
| GAGTCCCAAATTGGCAAAGA+AGG | - | chr7.1:21928638-21928657 | None:intergenic | 45.0% | |
| TGCCAAAGATTCCTTCAGGA+TGG | + | chr7.1:21927209-21927228 | MS.gene024226:CDS | 45.0% | |
| TTACCTCAGCACTAGGCAAA+CGG | - | chr7.1:21928040-21928059 | None:intergenic | 45.0% | |
| ! | AGATTTGGGTTCCATCCTGA+AGG | - | chr7.1:21927223-21927242 | None:intergenic | 45.0% |
| !!! | AATATTTTTTTTATGTAAAT+CGG | + | chr7.1:21927800-21927819 | MS.gene024226:intron | 5.0% |
| GTTGGATACGTGGGACAGTA+TGG | + | chr7.1:21928523-21928542 | MS.gene024226:CDS | 50.0% | |
| !! | ATGACGGTGTTGCTGATAGC+TGG | + | chr7.1:21928481-21928500 | MS.gene024226:CDS | 50.0% |
| TCTCCGTTTGCCTAGTGCTG+AGG | + | chr7.1:21928034-21928053 | MS.gene024226:intron | 55.0% | |
| AGCCACAGCCGTTGGATACG+TGG | + | chr7.1:21928513-21928532 | MS.gene024226:CDS | 60.0% | |
| GCCACAGCCGTTGGATACGT+GGG | + | chr7.1:21928514-21928533 | MS.gene024226:CDS | 60.0% | |
| TCCCACGTATCCAACGGCTG+TGG | - | chr7.1:21928518-21928537 | None:intergenic | 60.0% | |
| TGTTCAGCAGCCACAGCCGT+TGG | + | chr7.1:21928505-21928524 | MS.gene024226:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 21927201 | 21928690 | 21927201 | ID=MS.gene024226 |
| chr7.1 | mRNA | 21927201 | 21928690 | 21927201 | ID=MS.gene024226.t1;Parent=MS.gene024226 |
| chr7.1 | exon | 21927201 | 21927421 | 21927201 | ID=MS.gene024226.t1.exon1;Parent=MS.gene024226.t1 |
| chr7.1 | CDS | 21927201 | 21927421 | 21927201 | ID=cds.MS.gene024226.t1;Parent=MS.gene024226.t1 |
| chr7.1 | exon | 21927541 | 21927667 | 21927541 | ID=MS.gene024226.t1.exon2;Parent=MS.gene024226.t1 |
| chr7.1 | CDS | 21927541 | 21927667 | 21927541 | ID=cds.MS.gene024226.t1;Parent=MS.gene024226.t1 |
| chr7.1 | exon | 21928478 | 21928690 | 21928478 | ID=MS.gene024226.t1.exon3;Parent=MS.gene024226.t1 |
| chr7.1 | CDS | 21928478 | 21928690 | 21928478 | ID=cds.MS.gene024226.t1;Parent=MS.gene024226.t1 |
| Gene Sequence |
| Protein sequence |
