AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024558

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024558.t1 MTR_7g115340 97.345 452 12 0 40 491 59 510 0.0 891
MS.gene024558.t1 MTR_7g115340 98.416 442 7 0 50 491 1 442 0.0 883
MS.gene024558.t1 MTR_1g070380 57.235 463 167 13 48 490 116 567 2.88e-146 431
MS.gene024558.t1 MTR_1g070380 57.328 464 166 14 48 490 116 568 5.27e-145 427
MS.gene024558.t1 MTR_4g127380 41.918 365 180 8 84 437 32 375 1.93e-85 270
MS.gene024558.t1 MTR_2g038020 38.835 412 211 10 61 449 22 415 3.61e-82 263
MS.gene024558.t1 MTR_2g019090 25.641 351 220 8 95 437 311 628 4.23e-25 109
MS.gene024558.t1 MTR_2g019090 23.955 359 205 10 98 424 44 366 3.70e-14 75.5
MS.gene024558.t1 MTR_7g080630 33.728 169 104 2 97 257 101 269 2.54e-24 103
MS.gene024558.t1 MTR_7g080630 33.728 169 104 2 97 257 101 269 4.57e-24 102
MS.gene024558.t1 MTR_6g012630 36.257 171 99 4 95 255 46 216 4.93e-24 104
MS.gene024558.t1 MTR_6g012630 36.257 171 99 4 95 255 46 216 5.93e-24 105
MS.gene024558.t1 MTR_6g012630 36.257 171 99 4 95 255 46 216 7.79e-24 105
MS.gene024558.t1 MTR_7g080630 33.728 169 104 2 97 257 101 269 1.15e-23 102
MS.gene024558.t1 MTR_4g008080 26.289 388 226 14 95 437 226 598 1.04e-21 99.0
MS.gene024558.t1 MTR_7g011080 33.708 178 101 4 95 255 48 225 1.45e-21 98.2
MS.gene024558.t1 MTR_3g110650 25.281 356 238 9 97 437 313 655 3.68e-20 94.4
MS.gene024558.t1 MTR_4g093650 36.686 169 99 5 94 256 18 184 7.99e-18 87.0
MS.gene024558.t1 MTR_4g093650 23.797 374 237 12 95 442 265 616 9.46e-18 86.7
MS.gene024558.t1 MTR_5g031720 28.723 188 118 5 81 256 21 204 3.29e-16 81.6
MS.gene024558.t1 MTR_5g031720 24.931 361 215 11 97 442 276 595 8.73e-13 70.9
MS.gene024558.t1 MTR_2g019090 23.642 313 198 8 95 399 312 591 1.99e-15 79.3
MS.gene024558.t1 MTR_2g019090 23.677 359 207 9 98 424 44 367 4.26e-15 78.2
MS.gene024558.t1 MTR_2g009650 26.852 216 132 5 85 282 37 244 3.00e-14 75.9
MS.gene024558.t1 MTR_2g009650 24.706 170 123 1 95 259 330 499 5.74e-13 72.0
MS.gene024558.t1 MTR_4g116880 57.534 73 13 3 143 215 10 64 2.74e-13 65.5
MS.gene024558.t1 MTR_3g091800 23.926 163 117 2 97 259 332 487 9.04e-12 67.8
MS.gene024558.t1 MTR_3g091800 27.273 176 112 4 98 257 65 240 5.10e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024558.t1 AT3G04610 66.667 447 120 11 61 490 119 553 0.0 538
MS.gene024558.t1 AT3G04610 66.667 447 120 11 61 490 119 553 0.0 538
MS.gene024558.t1 AT3G04610 66.667 447 120 11 61 490 143 577 0.0 537
MS.gene024558.t1 AT3G04610 66.667 447 120 11 61 490 143 577 0.0 537
MS.gene024558.t1 AT4G26000 42.147 382 193 8 86 457 64 427 6.10e-91 286
MS.gene024558.t1 AT1G51580 26.923 364 223 10 95 437 275 616 1.68e-26 113
MS.gene024558.t1 AT5G15270 25.444 338 188 8 95 420 49 334 6.20e-24 105
MS.gene024558.t1 AT5G15270 25.683 183 132 3 81 262 270 449 1.92e-17 85.5
MS.gene024558.t1 AT5G15270 25.444 338 188 8 95 420 49 334 6.20e-24 105
MS.gene024558.t1 AT5G15270 25.683 183 132 3 81 262 270 449 1.92e-17 85.5
MS.gene024558.t1 AT5G15270 25.444 338 188 8 95 420 49 334 7.90e-24 105
MS.gene024558.t1 AT5G15270 25.683 183 132 3 81 262 270 449 2.41e-17 85.1
MS.gene024558.t1 AT4G18375 26.836 354 211 13 97 425 37 367 6.06e-19 90.1
MS.gene024558.t1 AT4G18375 26.462 359 206 13 97 425 37 367 1.01e-18 89.7
MS.gene024558.t1 AT1G14170 30.857 175 103 4 95 252 10 183 5.80e-18 86.7
MS.gene024558.t1 AT1G14170 29.651 172 113 4 100 267 240 407 2.81e-11 65.9
MS.gene024558.t1 AT1G14170 30.857 175 103 4 95 252 18 191 6.81e-18 86.7
MS.gene024558.t1 AT1G14170 29.651 172 113 4 100 267 248 415 3.03e-11 65.9
MS.gene024558.t1 AT1G14170 30.857 175 103 4 95 252 43 216 7.92e-18 86.3
MS.gene024558.t1 AT1G14170 29.651 172 113 4 100 267 273 440 2.45e-11 66.2
MS.gene024558.t1 AT5G64390 24.051 395 242 9 97 437 453 843 4.04e-17 85.1
MS.gene024558.t1 AT5G64390 28.293 205 120 5 94 281 45 239 1.63e-15 80.1
MS.gene024558.t1 AT5G53060 22.543 346 246 5 92 437 321 644 2.59e-16 82.4
MS.gene024558.t1 AT5G53060 25.510 196 122 5 85 257 57 251 5.21e-12 68.6
MS.gene024558.t1 AT5G64390 28.922 204 120 5 94 281 45 239 1.66e-15 80.1
MS.gene024558.t1 AT5G64390 25.434 173 122 2 97 264 453 623 1.55e-12 70.5
MS.gene024558.t1 AT5G64390 28.293 205 120 5 94 281 45 239 1.75e-15 80.1
MS.gene024558.t1 AT5G64390 25.434 173 122 2 97 264 453 623 1.60e-12 70.5
MS.gene024558.t1 AT5G46190 25.926 351 183 14 97 409 45 356 2.10e-13 73.2
MS.gene024558.t1 AT5G46190 25.926 351 183 14 97 409 45 356 2.87e-13 72.8
MS.gene024558.t1 AT5G46190 25.926 351 183 14 97 409 45 356 2.87e-13 72.8
MS.gene024558.t1 AT5G46190 25.926 351 183 14 97 409 45 356 2.87e-13 72.8
MS.gene024558.t1 AT5G53060 25.510 196 122 5 85 257 57 251 3.91e-12 68.9
MS.gene024558.t1 AT5G53060 25.000 168 122 2 92 259 321 484 2.24e-11 66.6

Find 177 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATACAATTTGATGCTTCT+TGG 0.178221 7.2:+2136677 None:intergenic
TCACAAGCAGGAAGTTTAAT+TGG 0.191863 7.2:-2136727 MS.gene024558:CDS
AGAGATGTAAATTAGAAGTT+TGG 0.227622 7.2:+2139674 None:intergenic
TCGTACGCTGATGCTGTTAT+TGG 0.256618 7.2:-2133926 MS.gene024558:CDS
GGAGCCTATGGTGCAGTTTA+TGG 0.267209 7.2:-2132822 MS.gene024558:CDS
GCAGTTTATGGTGCAAATTA+TGG 0.271012 7.2:-2132810 MS.gene024558:CDS
TGGTGGGGTGGTGGTGGCAC+TGG 0.284770 7.2:+2134578 None:intergenic
CAAAAGGTTGGTGGGATAAT+TGG 0.293067 7.2:-2139260 MS.gene024558:CDS
CACAGCCCGTTCTGAAGTTC+CGG 0.314112 7.2:+2139166 None:intergenic
GATATAGCTTATATTTGTTC+CGG 0.327385 7.2:+2133898 None:intergenic
GCTGGTGGAGGTCCTGGATT+TGG 0.327478 7.2:-2134521 MS.gene024558:CDS
ACGAAGTGATGAAGGATAAT+AGG 0.336901 7.2:+2139793 None:intergenic
GAAGATGGAATCTAGGGTTT+TGG 0.338192 7.2:+2139710 None:intergenic
CAGTTTATGGTGCAAATTAT+GGG 0.346952 7.2:-2132809 MS.gene024558:CDS
AACTCCTCTTGTTTCTTGTA+TGG 0.349928 7.2:+2133850 None:intergenic
CCCCATGATTGGTGGGGTGG+TGG 0.363121 7.2:+2134569 None:intergenic
GATGCTCCTTCAGGTGTGGC+TGG 0.367185 7.2:-2136781 MS.gene024558:CDS
GGCACTGGCATGTGCTCCAC+AGG 0.369095 7.2:+2134593 None:intergenic
GAATGAAGAAGATGGAATCT+AGG 0.369203 7.2:+2139703 None:intergenic
CAGCCCGGCCATTTCTTCTC+AGG 0.371179 7.2:+2139320 None:intergenic
CCAAATGCTGGTGGAGGTCC+TGG 0.372713 7.2:-2134527 MS.gene024558:CDS
CCAATCATGGGGTCCACCTC+AGG 0.379209 7.2:-2134558 MS.gene024558:CDS
CATGATTGGTGGGGTGGTGG+TGG 0.386854 7.2:+2134572 None:intergenic
GAGATGTAAATTAGAAGTTT+GGG 0.387707 7.2:+2139675 None:intergenic
GAGTAAATTACCTTGATTGC+AGG 0.388670 7.2:+2139649 None:intergenic
GCTCGTGTTAAGATTCTTGA+TGG 0.388997 7.2:-2139194 MS.gene024558:CDS
GATGGGTTAGATGCTCCTTC+AGG 0.390825 7.2:-2136790 MS.gene024558:CDS
GACAGGGTGGTTGAAGTAGT+AGG 0.398062 7.2:-2134817 MS.gene024558:CDS
GCTCATAAACGGTTAATTGA+TGG 0.400721 7.2:-2136808 MS.gene024558:CDS
CACTGCCTTGTGCACTCCAA+TGG 0.404296 7.2:+2134789 None:intergenic
AAACTTCCTGCTTGTGAGGC+TGG 0.410456 7.2:+2136733 None:intergenic
CACCACCACCCCACCAATCA+TGG 0.417160 7.2:-2134571 MS.gene024558:CDS
CTGAGAAGAAATGGCCGGGC+TGG 0.417620 7.2:-2139319 MS.gene024558:CDS
CCACCTGTTTGTTGCTGCGC+CGG 0.419210 7.2:+2132927 None:intergenic
GCACCGGCGCAGCAACAAAC+AGG 0.420300 7.2:-2132930 MS.gene024558:CDS
GAATTGTATCCTTGGTCTGC+AGG 0.425427 7.2:+2132900 None:intergenic
CCAGGACCTCCACCAGCATT+TGG 0.425866 7.2:+2134527 None:intergenic
TCTCTTCCACCTGCTGTGGA+TGG 0.429032 7.2:-2136841 MS.gene024558:CDS
AATGTTGGTTCCTGCTCAAA+AGG 0.429285 7.2:-2139276 MS.gene024558:CDS
TGCTGTGGATGGCTTATTGA+AGG 0.431392 7.2:-2136830 MS.gene024558:CDS
GCCTCCCGGAACTTCAGAAC+GGG 0.433855 7.2:-2139171 MS.gene024558:CDS
TCCTTCAGGTGTGGCTGGGA+AGG 0.436543 7.2:-2136776 MS.gene024558:CDS
TAATTATCAACTTGCCTTGG+AGG 0.437730 7.2:+2134476 None:intergenic
CACAAGCAGGAAGTTTAATT+GGG 0.440852 7.2:-2136726 MS.gene024558:CDS
AGCTGAAATGCCTCCTCCAT+TGG 0.441524 7.2:-2134444 MS.gene024558:CDS
AAGAAATGGCCGGGCTGGCC+TGG 0.444619 7.2:-2139314 MS.gene024558:CDS
AAATTGTATAGTCAGAGTTC+TGG 0.448147 7.2:-2136665 MS.gene024558:CDS
CAAGAAACAAGAGGAGTTCC+TGG 0.449581 7.2:-2133845 MS.gene024558:CDS
CAACCACCCTGTCATCTTGA+AGG 0.450885 7.2:+2134827 None:intergenic
GAGGTGGACCCCATGATTGG+TGG 0.454647 7.2:+2134561 None:intergenic
CCAGGACATGCTGGAGCCTA+TGG 0.454711 7.2:-2132834 MS.gene024558:CDS
ACAGGGTGGTTGAAGTAGTA+GGG 0.462789 7.2:-2134816 MS.gene024558:CDS
ACTGCCTTGTGCACTCCAAT+GGG 0.463713 7.2:+2134790 None:intergenic
AGGTGGACCCCATGATTGGT+GGG 0.466069 7.2:+2134562 None:intergenic
ACAGCCCGTTCTGAAGTTCC+GGG 0.466480 7.2:+2139167 None:intergenic
AAGATTCTTGATGGGCCTCC+CGG 0.466560 7.2:-2139185 MS.gene024558:CDS
AATTGTATAGTCAGAGTTCT+GGG 0.468142 7.2:-2136664 MS.gene024558:CDS
TCGTTGAGCCTGAGAAGAAA+TGG 0.468519 7.2:-2139328 MS.gene024558:CDS
GATCTTTCTCCAGATGCAAA+TGG 0.469181 7.2:-2134627 MS.gene024558:intron
CCATAGGCTCCAGCATGTCC+TGG 0.470428 7.2:+2132834 None:intergenic
GGACCCCATGATTGGTGGGG+TGG 0.475178 7.2:+2134566 None:intergenic
TATGGAAGAGATGCTTCAAT+AGG 0.476740 7.2:-2134392 MS.gene024558:CDS
GCTTATTGAAGGCTCATAAA+CGG 0.481382 7.2:-2136819 MS.gene024558:CDS
ACATCTCTACCCTGCAATCA+AGG 0.483716 7.2:-2139659 MS.gene024558:intron
CTCATAAACGGTTAATTGAT+GGG 0.485762 7.2:-2136807 MS.gene024558:CDS
TTCAATAAGCCATCCACAGC+AGG 0.487643 7.2:+2136832 None:intergenic
GTTCCTGCTCAAAAGGTTGG+TGG 0.488563 7.2:-2139269 MS.gene024558:CDS
ACAGCATCAGCGTACGATAG+AGG 0.489668 7.2:+2133932 None:intergenic
GGCTCCAGCATGTCCTGGAT+TGG 0.489729 7.2:+2132839 None:intergenic
TTCTTCTCTTCCACCTGCTG+TGG 0.494945 7.2:-2136845 MS.gene024558:CDS
TTCTTCTCAGGCTCAACGAT+CGG 0.495988 7.2:+2139332 None:intergenic
CATTGGAGTGCACAAGGCAG+TGG 0.499275 7.2:-2134788 MS.gene024558:CDS
CAGGGTAGGAATTGTATCCT+TGG 0.499993 7.2:+2132892 None:intergenic
AACAGCACAGCAATTGATAC+AGG 0.502245 7.2:-2133780 MS.gene024558:intron
ACCACCACCCCACCAATCAT+GGG 0.502652 7.2:-2134570 MS.gene024558:CDS
CTTGGAGGTGGCATGAATTG+AGG 0.503745 7.2:+2134491 None:intergenic
CCTGAGGTGGACCCCATGAT+TGG 0.504891 7.2:+2134558 None:intergenic
GCGTCACCACCTTCCAATCC+AGG 0.508103 7.2:-2132852 MS.gene024558:CDS
CATCATGGTATATCCGCCTA+TGG 0.508306 7.2:-2134410 MS.gene024558:CDS
ATGCTCCTTCAGGTGTGGCT+GGG 0.509317 7.2:-2136780 MS.gene024558:CDS
CAGGGTCTCCCACCAAATGC+TGG 0.510314 7.2:-2134539 MS.gene024558:CDS
CTCCCACCAAATGCTGGTGG+AGG 0.511292 7.2:-2134533 MS.gene024558:CDS
TTGTGAGAACGAAGTGATGA+AGG 0.516786 7.2:+2139785 None:intergenic
TATGGCGGAAGCTGCAGCAC+CGG 0.517885 7.2:-2132946 MS.gene024558:CDS
TACTCAGTCTGCACCTTCCA+TGG 0.518367 7.2:-2134351 MS.gene024558:CDS
ACACTCTCACCAGGCCAGCC+CGG 0.521278 7.2:+2139305 None:intergenic
TTATCAACTTGCCTTGGAGG+TGG 0.522949 7.2:+2134479 None:intergenic
AATTAAACTTCCTGCTTGTG+AGG 0.525068 7.2:+2136729 None:intergenic
CCAGCATGTCCTGGATTGGA+AGG 0.525197 7.2:+2132843 None:intergenic
AATGAAGAAGATGGAATCTA+GGG 0.525306 7.2:+2139704 None:intergenic
TAGTAATTATCAACTTGCCT+TGG 0.526071 7.2:+2134473 None:intergenic
GGGTGCGAGAATGAAGAAGA+TGG 0.528572 7.2:+2139695 None:intergenic
GGAACAAAATGAGTCTGAAA+AGG 0.528718 7.2:-2139366 MS.gene024558:CDS
ATTTCAACTGTCATCTCCCC+AGG 0.529708 7.2:+2133827 None:intergenic
AGATGACAGTTGAAATCAGC+GGG 0.541840 7.2:-2133820 MS.gene024558:CDS
CATAGGCGGATATACCATGA+TGG 0.542292 7.2:+2134411 None:intergenic
TTCTGGCAGTGGAAATTCCA+TGG 0.543536 7.2:-2139444 MS.gene024558:intron
CCTTCCAATCCAGGACATGC+TGG 0.543630 7.2:-2132843 MS.gene024558:CDS
CCGGCGCAGCAACAAACAGG+TGG 0.553534 7.2:-2132927 MS.gene024558:CDS
AATGGAGGAGGCATTTCAGC+TGG 0.554857 7.2:+2134446 None:intergenic
GGCTGTTTGTCCAATGGAGG+AGG 0.557347 7.2:+2134434 None:intergenic
ATAGTCAGAGTTCTGGGAGC+AGG 0.562698 7.2:-2136658 MS.gene024558:intron
GCATAGACAGAACTATGAGC+AGG 0.562952 7.2:+2132873 None:intergenic
ACCTTCCCAGCCACACCTGA+AGG 0.563518 7.2:+2136775 None:intergenic
CTCAATTCATGCCACCTCCA+AGG 0.565069 7.2:-2134490 MS.gene024558:CDS
TGTTTCTTGTATGGTAACAG+TGG 0.565082 7.2:+2133859 None:intergenic
TTCCTGCTCAAAAGGTTGGT+GGG 0.565402 7.2:-2139268 MS.gene024558:CDS
GAGCCTGAGAAGAAATGGCC+GGG 0.566112 7.2:-2139323 MS.gene024558:CDS
TGAGCCTGAGAAGAAATGGC+CGG 0.566245 7.2:-2139324 MS.gene024558:CDS
GAAAAGTACCTGTGTGACCA+TGG 0.567170 7.2:+2134334 None:intergenic
GGACCTCCACCAGCATTTGG+TGG 0.567350 7.2:+2134530 None:intergenic
TGAGGAGCAAGTCCAAATCC+AGG 0.567540 7.2:+2134509 None:intergenic
GGCCTCCCGGAACTTCAGAA+CGG 0.568253 7.2:-2139172 MS.gene024558:CDS
TGATGAAGGATAATAGGTGG+TGG 0.568994 7.2:+2139799 None:intergenic
ATAGGCGGATATACCATGAT+GGG 0.572279 7.2:+2134412 None:intergenic
GGAAGGTGCAGACTGAGTAT+TGG 0.573422 7.2:+2134355 None:intergenic
GCACTGGCATGTGCTCCACA+GGG 0.573640 7.2:+2134594 None:intergenic
TATTGAAGCATCTCTTCCAT+AGG 0.574272 7.2:+2134394 None:intergenic
CTCGTGTTAAGATTCTTGAT+GGG 0.574443 7.2:-2139193 MS.gene024558:CDS
AAGAAACAAGAGGAGTTCCT+GGG 0.578584 7.2:-2133844 MS.gene024558:CDS
GGAGGAGGCATTTCAGCTGG+TGG 0.580842 7.2:+2134449 None:intergenic
TTGGTTCCTGCTCAAAAGGT+TGG 0.581334 7.2:-2139272 MS.gene024558:CDS
AGTAAATTACCTTGATTGCA+GGG 0.581647 7.2:+2139650 None:intergenic
CTAGTGCCAGCCTCACAAGC+AGG 0.582418 7.2:-2136739 MS.gene024558:CDS
GGATCCCATTGGAGTGCACA+AGG 0.586468 7.2:-2134794 MS.gene024558:CDS
TTGGACAAACAGCCCCATCA+TGG 0.587088 7.2:-2134425 MS.gene024558:CDS
GATGCTGTTATTGGAACAGC+CGG 0.587215 7.2:-2133917 MS.gene024558:CDS
AACCACCCTGTCATCTTGAA+GGG 0.588607 7.2:+2134828 None:intergenic
GTATCAGCTAAAGATGAGCC+TGG 0.589213 7.2:-2136868 MS.gene024558:CDS
TGCACCATAAACTGCACCAT+AGG 0.591283 7.2:+2132818 None:intergenic
GTTCTGATCAACCTCAGCCA+TGG 0.592521 7.2:+2139427 None:intergenic
TTGTATCCTTGGTCTGCAGG+AGG 0.600150 7.2:+2132903 None:intergenic
GGTGGACCCCATGATTGGTG+GGG 0.600476 7.2:+2134563 None:intergenic
AGCTATATCAGAAGAGCAAG+TGG 0.600575 7.2:-2133884 MS.gene024558:CDS
TGATGGGGCTGTTTGTCCAA+TGG 0.602005 7.2:+2134428 None:intergenic
GACCTCCACCAGCATTTGGT+GGG 0.602365 7.2:+2134531 None:intergenic
GGTGGGATAATTGGTCGGAA+AGG 0.602653 7.2:-2139251 MS.gene024558:CDS
GACAGAACTATGAGCAGGGT+AGG 0.604594 7.2:+2132878 None:intergenic
CAGTGGAAATTCCATGGCTG+AGG 0.606695 7.2:-2139438 MS.gene024558:CDS
CAATCATGGGGTCCACCTCA+GGG 0.610026 7.2:-2134557 MS.gene024558:CDS
GCTATATCAGAAGAGCAAGT+GGG 0.610415 7.2:-2133883 MS.gene024558:CDS
AATAAGCCATCCACAGCAGG+TGG 0.611934 7.2:+2136835 None:intergenic
ACAAAATGAGTCTGAAAAGG+TGG 0.617900 7.2:-2139363 MS.gene024558:CDS
GGTCTCCCACCAAATGCTGG+TGG 0.624009 7.2:-2134536 MS.gene024558:CDS
AGTTTAATTGGGAAACAAGG+AGG 0.625389 7.2:-2136715 MS.gene024558:CDS
TGCACCTTCCATGGTCACAC+AGG 0.626823 7.2:-2134342 MS.gene024558:intron
CAGGGTGGTTGAAGTAGTAG+GGG 0.627870 7.2:-2134815 MS.gene024558:CDS
TGAAGCATCTCTTCCATAGG+CGG 0.628282 7.2:+2134397 None:intergenic
ATTCTGAAAACACTCTCACC+AGG 0.628905 7.2:+2139296 None:intergenic
TGCCCTTCAAGATGACAGGG+TGG 0.630655 7.2:-2134830 MS.gene024558:CDS
GAAGTAGTAGGGGATCCCAT+TGG 0.631279 7.2:-2134805 MS.gene024558:CDS
AGTACCTGTGTGACCATGGA+AGG 0.631574 7.2:+2134338 None:intergenic
GTTACCATACAAGAAACAAG+AGG 0.636417 7.2:-2133854 MS.gene024558:CDS
CTGGCATGTGCTCCACAGGG+TGG 0.637657 7.2:+2134597 None:intergenic
GGAAGTTTAATTGGGAAACA+AGG 0.639460 7.2:-2136718 MS.gene024558:CDS
AAGTGATGAAGGATAATAGG+TGG 0.639762 7.2:+2139796 None:intergenic
CTATATCAGAAGAGCAAGTG+GGG 0.641360 7.2:-2133882 MS.gene024558:CDS
GTTAGATGCTCCTTCAGGTG+TGG 0.645046 7.2:-2136785 MS.gene024558:CDS
GCAGGTGGAAGAGAAGAACC+AGG 0.646569 7.2:+2136850 None:intergenic
GAGGAGGCATTTCAGCTGGT+GGG 0.647020 7.2:+2134450 None:intergenic
CGGCGCAGCAACAAACAGGT+GGG 0.647966 7.2:-2132926 MS.gene024558:CDS
AGGTTGGTGGGATAATTGGT+CGG 0.649103 7.2:-2139256 MS.gene024558:CDS
CCACCACCCCACCAATCATG+GGG 0.649451 7.2:-2134569 MS.gene024558:CDS
ACAACAGCGAATTGTTGAGA+CGG 0.650385 7.2:-2139387 MS.gene024558:CDS
TTTGGTGGGAGACCCTGAGG+TGG 0.656048 7.2:+2134545 None:intergenic
GGTGGGCCTCCTGCAGACCA+AGG 0.658048 7.2:-2132909 MS.gene024558:CDS
GCATTTGGTGGGAGACCCTG+AGG 0.663331 7.2:+2134542 None:intergenic
TGGGGCTGTTTGTCCAATGG+AGG 0.664238 7.2:+2134431 None:intergenic
CATAGACAGAACTATGAGCA+GGG 0.665116 7.2:+2132874 None:intergenic
GCATGTCCTGGATTGGAAGG+TGG 0.687180 7.2:+2132846 None:intergenic
TAGGCGGATATACCATGATG+GGG 0.688165 7.2:+2134413 None:intergenic
GCTGAGGTTGATCAGAACGT+AGG 0.692033 7.2:-2139422 MS.gene024558:CDS
TGAGTCTGAAAAGGTGGTTG+AGG 0.692336 7.2:-2139357 MS.gene024558:CDS
GCCCGTTCTGAAGTTCCGGG+AGG 0.698554 7.2:+2139170 None:intergenic
AGAAACAAGAGGAGTTCCTG+GGG 0.720239 7.2:-2133843 MS.gene024558:CDS
AATGGCTAATAACCACCCTG+TGG 0.739150 7.2:-2134609 MS.gene024558:CDS
GAGATGACAGTTGAAATCAG+CGG 0.760601 7.2:-2133821 MS.gene024558:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.2 gene 2132800 2139824 2132800 ID=MS.gene024558
chr7.2 mRNA 2132800 2139824 2132800 ID=MS.gene024558.t1;Parent=MS.gene024558
chr7.2 exon 2139782 2139824 2139782 ID=MS.gene024558.t1.exon1;Parent=MS.gene024558.t1
chr7.2 CDS 2139782 2139824 2139782 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2139660 2139753 2139660 ID=MS.gene024558.t1.exon2;Parent=MS.gene024558.t1
chr7.2 CDS 2139660 2139753 2139660 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2139169 2139457 2139169 ID=MS.gene024558.t1.exon3;Parent=MS.gene024558.t1
chr7.2 CDS 2139169 2139457 2139169 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2136659 2136896 2136659 ID=MS.gene024558.t1.exon4;Parent=MS.gene024558.t1
chr7.2 CDS 2136659 2136896 2136659 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2134720 2134868 2134720 ID=MS.gene024558.t1.exon5;Parent=MS.gene024558.t1
chr7.2 CDS 2134720 2134868 2134720 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2134343 2134636 2134343 ID=MS.gene024558.t1.exon6;Parent=MS.gene024558.t1
chr7.2 CDS 2134343 2134636 2134343 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2133781 2133975 2133781 ID=MS.gene024558.t1.exon7;Parent=MS.gene024558.t1
chr7.2 CDS 2133781 2133975 2133781 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
chr7.2 exon 2132800 2132973 2132800 ID=MS.gene024558.t1.exon8;Parent=MS.gene024558.t1
chr7.2 CDS 2132800 2132973 2132800 ID=cds.MS.gene024558.t1;Parent=MS.gene024558.t1
Gene Sequence

>MS.gene024558

ATGCCACCACCTATTATCCTTCATCACTTCGTTCTCACAACTTGTAAGTCACTCCCTCCACTCTCACTTAGCCCCTTTTCCCAAAACCAATACCAAAACCCTAGATTCCATCTTCTTCATTCTCGCACCCAAACTTCTAATTTACATCTCTACCCTGCAATCAAGGTAATTTACTCACCATGTTCGTTTTTTCTATGCATTTATTGTACTCTACACTCACTTATTCACTATTTCTCGCGGTTCAGCAACTTTTTCTCGATAAATCCTTTACAGATCTATCCCCTATTCTCCTTTTAACTCTACTTCTACTTGTTGATATCATGTGGTGTAAAAATTTGATTTTTTTGTTTGTTTGTGTTTCTGGCAGTGGAAATTCCATGGCTGAGGTTGATCAGAACGTAGGTGAATTCGAAGAACAACAGCGAATTGTTGAGACGGAACAAAATGAGTCTGAAAAGGTGGTTGAGGTGCCGATCGTTGAGCCTGAGAAGAAATGGCCGGGCTGGCCTGGTGAGAGTGTTTTCAGAATGTTGGTTCCTGCTCAAAAGGTTGGTGGGATAATTGGTCGGAAAGGAGAGTTTATTAAGAAGATTGTTGAAGAGACACGTGCTCGTGTTAAGATTCTTGATGGGCCTCCCGGAACTTCAGAACGGGCTGTGAGTGTTTTCTCTATTCAGCTTACATCTGTTTTTCTGGTTAAAAAGTTAGCTGCATTTGTATCTTGGTAGCATTGATCTGGAATGATTTATTTTTACCAAGCTCTTTTGGCTTTTCTTACTTCCTTCATTTTTTAAAAGGATTCATTTGGTTTTGAATATAGTTTTAAAATTTGTGGTTGAGTTGTAGAATAATATTCATGATATCTGTTTTTTATGGAGCTCTGCAGTGATTGTACTCATTACTTGCAATGATGACATAGTTATATCTTTTATCTGTACTTTTACTAATCCTAGATAAGTACATTATAACAAATAATAAACGAAAAATGTATGCACTGATGTGTGTCTGAGCGTCGCATTTTCTAAGACAAGGACCAATGTGAGAAATATTTACGTATGTAATTAGGTATTTACCTATATAATTTGGGTCCAAAATGCTTGTAAATATTCAAAGTAGGAATTATTGGGAGTGAGGGAAAGTACCGTTAATTCTTTTCAAGGACCTGGAATTTGTGTCTGAAACAATTCATAGCAATTATCCATTTAACTATGTCTCTCTCTGCTTAAGATGTTATGATGAAGAATAAATTGTGGCTGTCTTTCCTTAAGCAATCTTAATTTGCAATGTCCTATTCTCACGACAAGAAATTGTAAGAATAAAAAATATAGCTTGTTTATGGTCAGAACAAAGTGAAACCTTATTCATGTGATACAATGAACACAAAAACTAAGATGAAGAAGACGAAACAAATTCGAAAAAGAAATATGGAAAAAATCCACGAAATATGAATTTAATTCTAACAGAAAATGAATCTGTGCAGGTTCAATATTCCATTTTTAAAGGCAACGACTCAATCATCAATTAGATCCCCAATATTGAAGAACCAAAGTTCCAGATGCAGGATCAGCATTGAGCATTGCAAAGTGCTGAAAGTTTATTAACACGTAGTCATTTTATATCAAAGTACCACGAAGCTCTGCATTCCAGCGGGTAAATTCAAAACCTGGATGATTGATGTCTTGCCGCAGATTCCTAGCAATGAAAAAAAGTTTATCACCAGCTCTAATACAATACCGAAAACAATAAATACCAAACCATAAGTATTTTGTTTGTCATACATGTTATCAAGAAAAAAGGATCCGAAGATTAACCAAGGCTATCAATTAATTTTAATTTTAACACATGGAGTATGTCTATGTTAATATTTGTTATGTTATCACAATAGTTCTTCAGTAGGAAATATTGGTAAAATTATAGCTATAATTAACTAAATGCACTGTAGTAAACCAAGACCCAATCAATAAAAAAAAATAGAAGACACTTCCACGGTGACATACATTTTAAATCACACGTCATATCACTTATCAATTATGTTCACGTATGACACACTTCCATGTTTCTAAAAGGTTTATCTAGGAAATTCCTTTCACGTTTTAATCGCCATTGGATATGAGTAGTAAAGAATGTTTCTTGTGGTAGTTGGAACTTGGTGATATTAAATATACTGGCAAAGGGTTGGGACAAAATGTTGCTTGTTTTCTTCCCTAACAAGAAACAGGTAGAGGGCTGGGGTAAGTTGCACGAGGTGCAGAAGTATTGCATAGATTGGCAGTGGACATAGAAAGGAAAACTGGTAGGGAGCGGGTGGGCGTTCTACTTAGACAACGAGTTCAGGCACATAATCTTCAAAATTATATTTTAGAATTCTCCATGTTTTTTTTTTCTTTTTCTGTTGTCGATAAAAGATGTTTATCAAAATATCTAAACTGTGCTTCAGTTATCTATCTGAAAACCAACATGCTGTTATATTTGGTACTTTACCACAAGCAGTTTGACATTTTAATTTTCAAAGATTGCAATTGGACGAGGATAGGTTAAATAGTAATGTAGAGTATTTGGGTGCAAGAGCTATTCCTCTTAATAATTCGGCCTTTAAGACTGTTACAAAATTGGTTTGGTGGTGAAGTGAAGGATTCCTTCTGGAATTTTTCCGACAGGTCATGATAATGTAGGTATTAATTGTGGAAGAATATTGGCTATTTAGCTGAATCTCCTATTTGTTGCATTTTTAATAATCAAAGTTGCTTGCATTTCATGCTATCTTACTGATCCCGAGATTGTAGGTGGCATGTAAAGGGCTTTGTAATTGGTGCTCTGGTGGCTTTTTATTGTTTGTCACCATATTATGTCTTCGTGTTTCATATTATCATTAGAGGAAATGTTGAGATCAAAGACAGGCTGATAGTGTTATTTGTTCTTTGTGATTAGGTAATGGTATCAGCTAAAGATGAGCCTGGTTCTTCTCTTCCACCTGCTGTGGATGGCTTATTGAAGGCTCATAAACGGTTAATTGATGGGTTAGATGCTCCTTCAGGTGTGGCTGGGAAGGTTTCTACAAAATTTCTAGTGCCAGCCTCACAAGCAGGAAGTTTAATTGGGAAACAAGGAGGCAACGTAAAATCTATCCAAGAAGCATCAAATTGTATAGTCAGAGTTCTGGGAGCAGGTATCTACTCATATTTGATTTAAAAACTTTCAATCTGATTGCTGGATTGTCAATTTTATTATTACTTAGCATTCTGTTATTCTGAGAAGTTCATCGTAGCTTGTATTACAAATTTTCCTTACCCTAATTTTATATCAGAGATAAGTTTGGGATTCATCCACTGGGTACTAGAGCTGTAATAGAGCAATAGTGTAAATATGCTTTTAAGTCTATTAACTGGGATGGTTCCAAGTTCCTCTTCCTATTCGTCAAACGTGTTTTGTTCTTGTCACTGTTTGGTTTATTACCATTTTTACTATTATTGATACTCCTCTCTTTAATTGTCGCTTTTGTAGAAGAATTTTGTTCCTAGAGGTTAATGAGTTTGATCCATATATGGTTTCGGATAGAACATTATGGCGTAATTTTATCCCTGTAGCCGACCCCACTTAGTGGGATAAGGCTTGGTTGTTGTTGTTACCAAGGTTGTTAAACGCACGCTGTAGATCGTGAGATCGTACGATCTCACACTTTCTTCTCCCTAAACCGTGCCGGCGCGGTGCGAAAATCGTTGAAGAGGGAGGCGCGGCCGGTGCAGAGGTAGATTGCAAGATCGTGGAAGCGGCGAGTTTTCTCACACCGAATTTGTACCTTTTAACCCATATGACCCCGGTTCGCGGAGAAGAGTTCGTTGACCAAAACGACATGCTTTGCAGCAGAAGAGAAATGGATGTTGAAGAGTCAAAACAGTTGAGCTTGCCTTTCACGCAGTCAATATTAAAAGCTGATTTATTATTTTTGGAAAGTGTGCCGCTAATTGCTTGTTATTCTTAATTTTATTTTATAATGCTTCTTTTACGGGAATTTTTATTTTTTAATGCTAATTTGCCAAGTTTACTTTTTTTAATGCTAGTAATTTGCTAAGTTTACTCTATTTTTAAAAAGATTTCATTGTTTTTAGCCTTAATAAATCTTATATTTAATATATTTTAAAAGCTTTATGCTAACAAATACTTAGATAGTTTTTTTTCTAATAAAAATTAATTAAATTGTTAATTAGTTATTTATTTAACATAAGTACTATTTAATTTAATCATATAATTTAATTATATAACACTATATGTAGTATTTTTTTTGTTTGAAGAAACATTCTATACTTAGTAATATTAAGTGTGTTTCTTAATTATAGTATAATCTTGTCTTTGGTGGGATCTTACGATCCTTGTTACGATCTCACTATTTACGATTTTTCTATACCCAAACGATCTTAGATGAGATCTCGATTTTGACAACCATGGTTGTTACTGTGATTGTTTTCAAAGCTCAATGAATGAGATAATAAATAATCAAGAATATTAAGGAATAAGACATGATTTCATAAAAAATAATAAAATTTATTGATTGTATTAATATGTGTAAATACCCTTAAAACAACAAGTTAAAAGAAACGGAGGGAGTATCATGTAAGCACTCAGATATGCATAGCTAACTTCTATCATTACCACCTAAACCCTAAACTTACGATAATTCCCGCTATTTATTAATTCAGTTGTCAGATTCATGTATTCTAATCTAAGTAGTTTAAAACTTTCCCTTTCTAAAACCTTTTTTTGTCTATCCTTTTCATTAAAAATTATTTCTAAGATACAATTTGAAGTTTATGCTTCCAGTCTTCTGTAGTTTATGCTTTTAGATAAAATATTAGTGGAAATTTGAAGTTTTTTTCTCTTTCCGTTTTGGTTCTTGGATGGGCTGTTTGACATTCTCTAGTTGCTAAGCATCTTGCACCTCTGTTGTTTTTTTGTCTGCAGAAGATCTGCCTGTTTTTGCCCTTCAAGATGACAGGGTGGTTGAAGTAGTAGGGGATCCCATTGGAGTGCACAAGGCAGTGGAGATGATTGCATGCCACCTTAAGAAATTTTTAGTTGATCGCAGTATAATTCCAATTTTTGAAATGAATGTAAGTGTGTTAATTCATATTACACTTGTGAAGAATCTATCTTATGCTTACCAAGGAATGCAACTAATTTGATCTTTCTCCAGATGCAAATGGCTAATAACCACCCTGTGGAGCACATGCCAGTGCCACCACCACCCCACCAATCATGGGGTCCACCTCAGGGTCTCCCACCAAATGCTGGTGGAGGTCCTGGATTTGGACTTGCTCCTCAATTCATGCCACCTCCAAGGCAAGTTGATAATTACTACCCACCAGCTGAAATGCCTCCTCCATTGGACAAACAGCCCCATCATGGTATATCCGCCTATGGAAGAGATGCTTCAATAGGAGTTCACGCATCATCCAATACTCAGTCTGCACCTTCCATGGTCACACAGGTACTTTTCTTATTTCAAATTTTGTTTTCTAATAATCCCCAAAAGGAAGTAGTATGGTGATTGGGATGATATATTGGCATTACATTTTTATGTAGTTTGCATTTTTCTGGATGTCTTAGAACTCGCCAGTCTGTCTGTCGTTGTTTATGTAGTTTGATGAGACGACTTGTTGACTGCTGCTTCATGAATTTTGTGCAATAGCCGTGGTAAAGGCTGGTTGTGAAACTTTACCAGTGTTGATTGATATTTGCTGTTGCTTTCCACGTTGCTGTTTAGATTTAAGATAGCAGAGGGTGATGTTAGGTTCCACACTTGGATGTTAAGCTGAAATGATACTAATTGTCTTGCTAATGCTGTTGTGGCACAGATCACGCAGCAAATGCAAATTCCTCTATCGTACGCTGATGCTGTTATTGGAACAGCCGGAACAAATATAAGCTATATCAGAAGAGCAAGTGGGGCCACTGTTACCATACAAGAAACAAGAGGAGTTCCTGGGGAGATGACAGTTGAAATCAGCGGGACTGCTTCTCAAGTTCAAACAGCACAGCAATTGATACAGGTAATCCTGATCTTGGTCTTTGATTTGCTCCAGCCGTTCTAATATTAGTGTTGTCCTAGGTTTTCTTCAGACTAGGAAAAACTATAAATGAATAAAATAATATTGTAATTTTACAAACTTTTACTCTTTTTTTTTTTTTGAAGAAGCTAAATTAGCCCACCCTAATTGGCAAACTTTTCCTCATTAACAGTTGGTTATTAACCATTGTTATACTTGGAGGTAGAATTGGAATGAAATAATTAATGCCACTATTAAAAAACATAACAGTTATTTTAAGACATTTTTATATGTTGAAGCTGCATGCATTTTAGTTTATACATTACAGTTCATAGCATGCGGCATGTCTTGGAAATTTGTTTTAGATTTATACTGAATTAGAGTAATGAAAAAAATATTGAATCGTTTACTTGGTTCCTAAAAGATTCATTTTGCGCTAGAGTTTTATCGAAGATTAATTTTTTATGTAAATACAGAGAGTAAATTATAAGATTGTTAAATTTCTAAATTAAACTATTATCCTTCCACGAGTATTTTTTCAATAAGTTGACTGGTTGAGTAAGAAATTTGTCATTTTATCCATCCCCAGCCCGTCATTTATTTGATGCAAGGAAATTATCTTCAATCTTTTCTGTTATTTCATTTCATGCAGTATCTTTATTTTCTCAATTTGTTTCCATATAAAAGCTATGAATTTACTGTGCATAGTCCTATCTCACCGAATGCATGTCTTGGATGTTAGAACTGATTGACCTAACATTATCAATTTGCCTTGTACTCCAGCAATATAATCCTGAATCTGTGGTGCAGAATTTTATGGCGGAAGCTGCAGCACCGGCGCAGCAACAAACAGGTGGGCCTCCTGCAGACCAAGGATACAATTCCTACCCTGCTCATAGTTCTGTCTATGCGTCACCACCTTCCAATCCAGGACATGCTGGAGCCTATGGTGCAGTTTATGGTGCAAATTATGGGTACCAATGA
Protein sequence

>MS.gene024558.t1

MPPPIILHHFVLTTSPFPKTNTKTLDSIFFILAPKLLIYISTLQSSGNSMAEVDQNVGEFEEQQRIVETEQNESEKVVEVPIVEPEKKWPGWPGESVFRMLVPAQKVGGIIGRKGEFIKKIVEETRARVKILDGPPGTSERAVMVSAKDEPGSSLPPAVDGLLKAHKRLIDGLDAPSGVAGKVSTKFLVPASQAGSLIGKQGGNVKSIQEASNCIVRVLGAEDLPVFALQDDRVVEVVGDPIGVHKAVEMIACHLKKFLVDRSIIPIFEMNMQMANNHPVEHMPVPPPPHQSWGPPQGLPPNAGGGPGFGLAPQFMPPPRQVDNYYPPAEMPPPLDKQPHHGISAYGRDASIGVHASSNTQSAPSMVTQITQQMQIPLSYADAVIGTAGTNISYIRRASGATVTIQETRGVPGEMTVEISGTASQVQTAQQLIQNFMAEAAAPAQQQTGGPPADQGYNSYPAHSSVYASPPSNPGHAGAYGAVYGANYGYQ