Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026707.t1 | XP_013466382.1 | 85.5 | 62 | 9 | 0 | 1 | 62 | 1 | 62 | 1.20E-08 | 68.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026707.t1 | A0A072VG86 | 85.5 | 62 | 9 | 0 | 1 | 62 | 1 | 62 | 8.6e-09 | 68.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene026707 | MS.gene55776 | PPI |
| MS.gene026707 | MS.gene048906 | PPI |
| MS.gene026707 | MS.gene06050 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026707.t1 | MTR_1g028610 | 95.161 | 62 | 3 | 0 | 1 | 62 | 1 | 62 | 6.98e-29 | 97.4 |
| MS.gene026707.t1 | MTR_3g090830 | 77.966 | 59 | 11 | 1 | 4 | 62 | 7 | 63 | 9.14e-25 | 87.0 |
| MS.gene026707.t1 | MTR_6g086390 | 41.667 | 60 | 35 | 0 | 3 | 62 | 5 | 64 | 2.74e-11 | 53.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026707.t1 | AT2G46330 | 66.000 | 50 | 15 | 1 | 13 | 62 | 15 | 62 | 6.39e-16 | 65.1 |
| MS.gene026707.t1 | AT3G61640 | 57.143 | 56 | 22 | 1 | 7 | 62 | 9 | 62 | 1.68e-15 | 63.9 |
| MS.gene026707.t1 | AT5G53250 | 76.923 | 39 | 9 | 0 | 24 | 62 | 25 | 63 | 9.63e-15 | 61.6 |
Find 13 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCACTTACATCATTCATTA+AGG | 0.232380 | 1.2:-14937469 | None:intergenic |
| TCACTTGTGGGAGCGGGAGC+AGG | 0.433526 | 1.2:+14938283 | None:intergenic |
| GAAGCAAAGCAACCAGCATT+AGG | 0.447824 | 1.2:+14937491 | None:intergenic |
| GTGGGAGCGGGAGCAGGAGC+CGG | 0.473296 | 1.2:+14938289 | None:intergenic |
| TTCTTCACAGTCTGTTGCTC+CGG | 0.477404 | 1.2:-14938308 | MS.gene026707:CDS |
| TTAGGACATATGCTATTGCT+TGG | 0.507960 | 1.2:+14937509 | None:intergenic |
| AATAGCATATGTCCTAATGC+TGG | 0.553636 | 1.2:-14937503 | MS.gene026707:CDS |
| CATACCATCACTTGTGGGAG+CGG | 0.554416 | 1.2:+14938276 | None:intergenic |
| ATACCATCACTTGTGGGAGC+GGG | 0.556858 | 1.2:+14938277 | None:intergenic |
| GAAAACATACCATCACTTGT+GGG | 0.559175 | 1.2:+14938271 | None:intergenic |
| TGAAAACATACCATCACTTG+TGG | 0.566489 | 1.2:+14938270 | None:intergenic |
| GCTCCCGCTCCCACAAGTGA+TGG | 0.569380 | 1.2:-14938280 | MS.gene026707:intron |
| AAGATGCATAAATAAGACCA+AGG | 0.662451 | 1.2:+14938344 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAGAAAAATAACATAATT+AGG | + | chr1.2:14937696-14937715 | None:intergenic | 15.0% |
| !! | TCAATCAAAAACTTTAAATA+AGG | - | chr1.2:14937842-14937861 | MS.gene026707:intron | 15.0% |
| !! | AATTGTTCTTAAAAATTTGC+AGG | - | chr1.2:14938300-14938319 | MS.gene026707:CDS | 20.0% |
| !!! | TGTCACAATTTGATATTTTT+GGG | - | chr1.2:14938203-14938222 | MS.gene026707:intron | 20.0% |
| !!! | TTTTTTAACAATCCTAAACA+TGG | + | chr1.2:14938090-14938109 | None:intergenic | 20.0% |
| ! | AAATAGATCTAGACTATCTT+GGG | - | chr1.2:14938045-14938064 | MS.gene026707:intron | 25.0% |
| ! | AATAACAGTCTCATTCTTAT+CGG | + | chr1.2:14938139-14938158 | None:intergenic | 25.0% |
| ! | TATCCATGAATCATATAAGA+TGG | + | chr1.2:14937668-14937687 | None:intergenic | 25.0% |
| !! | CTGTCACAATTTGATATTTT+TGG | - | chr1.2:14938202-14938221 | MS.gene026707:intron | 25.0% |
| !!! | ATATTTTTGGGTTTAGTCTT+TGG | - | chr1.2:14938215-14938234 | MS.gene026707:intron | 25.0% |
| !!! | TTCTTATCGGTTCAATTTAA+CGG | + | chr1.2:14938126-14938145 | None:intergenic | 25.0% |
| AACAACAACATTGGATAGAA+CGG | + | chr1.2:14938251-14938270 | None:intergenic | 30.0% | |
| AAGATGCATAAATAAGACCA+AGG | + | chr1.2:14937503-14937522 | None:intergenic | 30.0% | |
| CAAAAAAGTTTCTCAACATG+AGG | + | chr1.2:14937806-14937825 | None:intergenic | 30.0% | |
| CAAATAGATCTAGACTATCT+TGG | - | chr1.2:14938044-14938063 | MS.gene026707:intron | 30.0% | |
| CAAGATAGTCTAGATCTATT+TGG | + | chr1.2:14938046-14938065 | None:intergenic | 30.0% | |
| TCACCATCTTATATGATTCA+TGG | - | chr1.2:14937662-14937681 | MS.gene026707:intron | 30.0% | |
| ! | TTCTATCCAATGTTGTTGTT+TGG | - | chr1.2:14938251-14938270 | MS.gene026707:intron | 30.0% |
| AATAGCATATGTCCTAATGC+TGG | - | chr1.2:14938341-14938360 | MS.gene026707:CDS | 35.0% | |
| GAAAACATACCATCACTTGT+GGG | + | chr1.2:14937576-14937595 | None:intergenic | 35.0% | |
| GAAGTCACACCAATAATGTA+TGG | - | chr1.2:14937934-14937953 | MS.gene026707:intron | 35.0% | |
| GAATCGCAACCATACATTAT+TGG | + | chr1.2:14937946-14937965 | None:intergenic | 35.0% | |
| GCAATTTAGACTACAACATC+TGG | - | chr1.2:14937874-14937893 | MS.gene026707:intron | 35.0% | |
| TGAAAACATACCATCACTTG+TGG | + | chr1.2:14937577-14937596 | None:intergenic | 35.0% | |
| TTAGGACATATGCTATTGCT+TGG | + | chr1.2:14938338-14938357 | None:intergenic | 35.0% | |
| TTGCAACCAAACAACAACAT+TGG | + | chr1.2:14938260-14938279 | None:intergenic | 35.0% | |
| ! | TTATGCATCTTTTGCGTCAA+TGG | - | chr1.2:14937512-14937531 | MS.gene026707:CDS | 35.0% |
| CAATGACCACAATGTGATTC+TGG | - | chr1.2:14937909-14937928 | MS.gene026707:intron | 40.0% | |
| CTTCAACCAGAATCACATTG+TGG | + | chr1.2:14937918-14937937 | None:intergenic | 40.0% | |
| GTCTATAACGCTCCATGTTT+AGG | - | chr1.2:14938075-14938094 | MS.gene026707:intron | 40.0% | |
| TGGCAAAGAAACACACTCAA+AGG | + | chr1.2:14938026-14938045 | None:intergenic | 40.0% | |
| TTGTTTGGTTGCAAAACGCA+TGG | - | chr1.2:14938266-14938285 | MS.gene026707:intron | 40.0% | |
| GAAGCAAAGCAACCAGCATT+AGG | + | chr1.2:14938356-14938375 | None:intergenic | 45.0% | |
| TTCTTCACAGTCTGTTGCTC+CGG | - | chr1.2:14937536-14937555 | MS.gene026707:intron | 45.0% | |
| ATACCATCACTTGTGGGAGC+GGG | + | chr1.2:14937570-14937589 | None:intergenic | 50.0% | |
| CATACCATCACTTGTGGGAG+CGG | + | chr1.2:14937571-14937590 | None:intergenic | 50.0% | |
| !! | TTTTGGGTGGTCGTGTTCCT+TGG | - | chr1.2:14937483-14937502 | MS.gene026707:CDS | 50.0% |
| GCTCCCGCTCCCACAAGTGA+TGG | - | chr1.2:14937564-14937583 | MS.gene026707:intron | 65.0% | |
| TCACTTGTGGGAGCGGGAGC+AGG | + | chr1.2:14937564-14937583 | None:intergenic | 65.0% | |
| GTGGGAGCGGGAGCAGGAGC+CGG | + | chr1.2:14937558-14937577 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 14937471 | 14938395 | 14937471 | ID=MS.gene026707 |
| chr1.2 | mRNA | 14937471 | 14938395 | 14937471 | ID=MS.gene026707.t1;Parent=MS.gene026707 |
| chr1.2 | exon | 14938281 | 14938395 | 14938281 | ID=MS.gene026707.t1.exon1;Parent=MS.gene026707.t1 |
| chr1.2 | CDS | 14938281 | 14938395 | 14938281 | ID=cds.MS.gene026707.t1;Parent=MS.gene026707.t1 |
| chr1.2 | exon | 14937471 | 14937544 | 14937471 | ID=MS.gene026707.t1.exon2;Parent=MS.gene026707.t1 |
| chr1.2 | CDS | 14937471 | 14937544 | 14937471 | ID=cds.MS.gene026707.t1;Parent=MS.gene026707.t1 |
| Gene Sequence |
| Protein sequence |
