Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027218.t1 | XP_013455531.1 | 88.5 | 392 | 41 | 3 | 1 | 392 | 1 | 388 | 5.00E-185 | 657.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027218.t1 | Q9S7T8 | 53.7 | 389 | 175 | 5 | 5 | 390 | 4 | 390 | 8.2e-112 | 405.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027218.t1 | A0A072UIQ8 | 88.5 | 392 | 41 | 3 | 1 | 392 | 1 | 388 | 3.6e-185 | 657.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027218 | MS.gene065944 | PPI |
| MS.gene065945 | MS.gene027218 | PPI |
| MS.gene031697 | MS.gene027218 | PPI |
| MS.gene027218 | MS.gene045585 | PPI |
| MS.gene006610 | MS.gene027218 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027218.t1 | MTR_4g045707 | 88.520 | 392 | 41 | 3 | 1 | 392 | 1 | 388 | 0.0 | 685 |
| MS.gene027218.t1 | MTR_3g101010 | 72.423 | 388 | 105 | 2 | 5 | 392 | 4 | 389 | 0.0 | 535 |
| MS.gene027218.t1 | MTR_3g018740 | 68.734 | 387 | 116 | 4 | 5 | 390 | 4 | 386 | 0.0 | 516 |
| MS.gene027218.t1 | MTR_8g027395 | 66.925 | 387 | 122 | 3 | 6 | 392 | 17 | 397 | 1.54e-179 | 505 |
| MS.gene027218.t1 | MTR_3g048000 | 64.844 | 384 | 114 | 2 | 7 | 390 | 28 | 390 | 6.30e-177 | 498 |
| MS.gene027218.t1 | MTR_3g101030 | 65.450 | 411 | 115 | 9 | 4 | 392 | 24 | 429 | 6.56e-176 | 497 |
| MS.gene027218.t1 | MTR_8g461160 | 63.706 | 394 | 137 | 4 | 1 | 390 | 1 | 392 | 3.88e-168 | 476 |
| MS.gene027218.t1 | MTR_4g088525 | 66.485 | 367 | 117 | 4 | 25 | 391 | 8 | 368 | 4.33e-168 | 475 |
| MS.gene027218.t1 | MTR_2g045980 | 65.039 | 389 | 127 | 5 | 5 | 390 | 4 | 386 | 7.87e-168 | 475 |
| MS.gene027218.t1 | MTR_8g027420 | 63.307 | 387 | 125 | 4 | 6 | 392 | 6 | 375 | 3.12e-167 | 473 |
| MS.gene027218.t1 | MTR_3g101180 | 61.905 | 399 | 142 | 5 | 1 | 390 | 1 | 398 | 1.33e-166 | 472 |
| MS.gene027218.t1 | MTR_2g046030 | 64.010 | 389 | 131 | 5 | 5 | 390 | 4 | 386 | 1.52e-166 | 471 |
| MS.gene027218.t1 | MTR_7g050750 | 62.183 | 394 | 139 | 5 | 5 | 392 | 7 | 396 | 3.57e-165 | 468 |
| MS.gene027218.t1 | MTR_7g451890 | 61.125 | 391 | 146 | 4 | 6 | 392 | 14 | 402 | 1.66e-162 | 462 |
| MS.gene027218.t1 | MTR_7g047780 | 61.637 | 391 | 144 | 4 | 6 | 392 | 14 | 402 | 6.73e-161 | 458 |
| MS.gene027218.t1 | MTR_3g101130 | 60.202 | 397 | 145 | 6 | 1 | 392 | 1 | 389 | 8.29e-160 | 454 |
| MS.gene027218.t1 | MTR_7g050810 | 60.614 | 391 | 148 | 4 | 6 | 392 | 16 | 404 | 1.80e-156 | 447 |
| MS.gene027218.t1 | MTR_4g088535 | 61.299 | 385 | 125 | 5 | 4 | 387 | 5 | 366 | 1.09e-154 | 441 |
| MS.gene027218.t1 | MTR_3g101050 | 61.111 | 396 | 123 | 8 | 4 | 391 | 25 | 397 | 5.93e-148 | 425 |
| MS.gene027218.t1 | MTR_3g015620 | 59.640 | 389 | 127 | 6 | 6 | 390 | 16 | 378 | 7.31e-142 | 409 |
| MS.gene027218.t1 | MTR_3g102920 | 57.033 | 391 | 126 | 7 | 4 | 390 | 56 | 408 | 2.19e-135 | 393 |
| MS.gene027218.t1 | MTR_3g101200 | 56.474 | 363 | 129 | 6 | 30 | 390 | 55 | 390 | 4.07e-127 | 372 |
| MS.gene027218.t1 | MTR_3g101190 | 50.954 | 367 | 111 | 4 | 28 | 392 | 13 | 312 | 3.57e-117 | 343 |
| MS.gene027218.t1 | MTR_3g100995 | 67.181 | 259 | 80 | 4 | 134 | 392 | 11 | 264 | 5.89e-117 | 341 |
| MS.gene027218.t1 | MTR_3g018747 | 65.267 | 262 | 73 | 5 | 130 | 390 | 9 | 253 | 9.78e-109 | 320 |
| MS.gene027218.t1 | MTR_3g100010 | 55.732 | 314 | 89 | 4 | 31 | 311 | 42 | 338 | 1.52e-105 | 315 |
| MS.gene027218.t1 | MTR_7g050830 | 53.165 | 316 | 113 | 5 | 6 | 299 | 16 | 318 | 4.97e-102 | 305 |
| MS.gene027218.t1 | MTR_3g015760 | 60.000 | 270 | 102 | 4 | 122 | 390 | 15 | 279 | 2.88e-97 | 291 |
| MS.gene027218.t1 | MTR_3g111160 | 68.304 | 224 | 65 | 6 | 171 | 392 | 1 | 220 | 9.05e-91 | 273 |
| MS.gene027218.t1 | MTR_3g100520 | 61.883 | 223 | 70 | 3 | 171 | 392 | 1 | 209 | 1.63e-88 | 266 |
| MS.gene027218.t1 | MTR_3g114310 | 41.117 | 394 | 211 | 9 | 12 | 392 | 25 | 410 | 2.79e-87 | 271 |
| MS.gene027218.t1 | MTR_2g046410 | 66.667 | 207 | 63 | 4 | 187 | 390 | 144 | 347 | 1.03e-84 | 262 |
| MS.gene027218.t1 | MTR_3g099970 | 65.657 | 198 | 49 | 4 | 130 | 327 | 20 | 198 | 1.52e-81 | 248 |
| MS.gene027218.t1 | MTR_3g101110 | 51.452 | 241 | 85 | 5 | 130 | 366 | 68 | 280 | 5.77e-68 | 216 |
| MS.gene027218.t1 | MTR_3g111150 | 57.062 | 177 | 52 | 1 | 8 | 184 | 1 | 153 | 5.42e-61 | 194 |
| MS.gene027218.t1 | MTR_3g101150 | 64.557 | 158 | 51 | 3 | 237 | 390 | 1 | 157 | 7.50e-60 | 191 |
| MS.gene027218.t1 | MTR_3g101020 | 56.614 | 189 | 53 | 4 | 204 | 392 | 1 | 160 | 1.42e-58 | 188 |
| MS.gene027218.t1 | MTR_3g102870 | 62.581 | 155 | 56 | 2 | 195 | 349 | 3 | 155 | 2.78e-57 | 185 |
| MS.gene027218.t1 | MTR_3g100990 | 64.122 | 131 | 47 | 0 | 4 | 134 | 3 | 133 | 8.52e-48 | 170 |
| MS.gene027218.t1 | MTR_4g093550 | 64.286 | 84 | 30 | 0 | 6 | 89 | 6 | 89 | 8.47e-29 | 108 |
| MS.gene027218.t1 | MTR_3g101140 | 64.286 | 70 | 22 | 2 | 325 | 392 | 48 | 116 | 1.80e-20 | 86.7 |
| MS.gene027218.t1 | MTR_6g046890 | 52.727 | 110 | 38 | 3 | 14 | 109 | 7 | 116 | 4.04e-20 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027218.t1 | AT1G47710 | 53.728 | 389 | 175 | 5 | 5 | 390 | 4 | 390 | 8.51e-145 | 417 |
| MS.gene027218.t1 | AT1G47710 | 53.728 | 389 | 175 | 5 | 5 | 390 | 31 | 417 | 4.10e-144 | 416 |
| MS.gene027218.t1 | AT2G25240 | 47.826 | 391 | 184 | 9 | 6 | 389 | 5 | 382 | 6.91e-118 | 348 |
| MS.gene027218.t1 | AT3G45220 | 45.477 | 398 | 191 | 7 | 6 | 389 | 5 | 390 | 3.61e-117 | 346 |
| MS.gene027218.t1 | AT2G26390 | 44.836 | 397 | 191 | 9 | 6 | 389 | 5 | 386 | 2.18e-109 | 326 |
| MS.gene027218.t1 | AT1G62170 | 42.857 | 392 | 194 | 6 | 6 | 391 | 66 | 433 | 9.14e-102 | 308 |
| MS.gene027218.t1 | AT1G64030 | 43.077 | 390 | 195 | 6 | 5 | 389 | 4 | 371 | 7.24e-99 | 299 |
| MS.gene027218.t1 | AT2G14540 | 44.216 | 389 | 190 | 7 | 5 | 389 | 32 | 397 | 1.23e-98 | 300 |
| MS.gene027218.t1 | AT1G62170 | 39.765 | 425 | 193 | 7 | 6 | 391 | 66 | 466 | 1.17e-94 | 291 |
| MS.gene027218.t1 | AT2G35580 | 43.784 | 370 | 176 | 9 | 29 | 389 | 25 | 371 | 3.10e-92 | 282 |
| MS.gene027218.t1 | AT1G64010 | 49.315 | 219 | 87 | 5 | 171 | 389 | 1 | 195 | 4.66e-64 | 203 |
| MS.gene027218.t1 | AT1G64010 | 48.529 | 204 | 81 | 5 | 186 | 389 | 2 | 181 | 3.07e-56 | 183 |
| MS.gene027218.t1 | AT1G62160 | 39.423 | 208 | 88 | 6 | 193 | 391 | 42 | 220 | 6.99e-34 | 125 |
| MS.gene027218.t1 | AT1G63280 | 53.043 | 115 | 51 | 2 | 130 | 243 | 8 | 120 | 3.50e-32 | 118 |
| MS.gene027218.t1 | AT1G51330 | 50.893 | 112 | 51 | 3 | 129 | 237 | 30 | 140 | 2.29e-26 | 105 |
| MS.gene027218.t1 | AT1G64020 | 37.288 | 118 | 67 | 4 | 7 | 120 | 6 | 120 | 4.95e-17 | 77.0 |
Find 103 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGGTTCAATATCTCATT+TGG | 0.243686 | 3.4:-46019211 | MS.gene027218:CDS |
| GTTGAATAACTCCTTTAATA+AGG | 0.260616 | 3.4:+46019605 | None:intergenic |
| CAGTGCCTTCTTCGTTTACT+TGG | 0.271310 | 3.4:+46019056 | None:intergenic |
| GAGACAAATGGCCTTATTAA+AGG | 0.273581 | 3.4:-46019616 | MS.gene027218:CDS |
| TTACTTGGATAAAAGACTTT+TGG | 0.279450 | 3.4:+46019071 | None:intergenic |
| AATCAGAAGAAACGATCTTT+TGG | 0.302672 | 3.4:+46020043 | None:intergenic |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.322054 | 3.4:-46018896 | MS.gene027218:CDS |
| ACATATTACATACCTTGTTC+TGG | 0.328120 | 3.4:+46019992 | None:intergenic |
| CCTCGCCTCTCTGTCGCTAA+TGG | 0.342690 | 3.4:-46020105 | MS.gene027218:CDS |
| GGAAAGAAGTTTGAAGAGAA+AGG | 0.354278 | 3.4:+46020064 | None:intergenic |
| TCCCGGAGGTTTGACTAAAA+TGG | 0.356212 | 3.4:-46019137 | MS.gene027218:CDS |
| TAATAATGGCCTAGTTTATA+AGG | 0.358860 | 3.4:+46019379 | None:intergenic |
| AGAATTGACGGTCATAATAA+TGG | 0.381177 | 3.4:+46019365 | None:intergenic |
| ACCGGCACAATCCTCTTTGT+TGG | 0.389726 | 3.4:-46018920 | MS.gene027218:CDS |
| AACCCATGAATTGACTTCAT+TGG | 0.393781 | 3.4:+46019645 | None:intergenic |
| CACAATCCTCTTTGTTGGAC+AGG | 0.397313 | 3.4:-46018915 | MS.gene027218:CDS |
| GAAAGAAGTTTGAAGAGAAA+GGG | 0.401934 | 3.4:+46020065 | None:intergenic |
| CAAGGTTCAATATCTCATTT+GGG | 0.405007 | 3.4:-46019210 | MS.gene027218:CDS |
| TTTGAGTGAAACGTTGGTTC+GGG | 0.409970 | 3.4:+46020344 | None:intergenic |
| GAGAGTCCATCTTTCGCATC+TGG | 0.410672 | 3.4:+46019325 | None:intergenic |
| CTTCAACTTCCTTATAAACT+AGG | 0.412556 | 3.4:-46019388 | MS.gene027218:CDS |
| CCCTCTACTTCAAAGGAGTA+TGG | 0.413172 | 3.4:-46019540 | MS.gene027218:CDS |
| AAAATGGTGGACTCACTTGC+CGG | 0.418030 | 3.4:-46019121 | MS.gene027218:CDS |
| GAAAGATGGACTCTCAGATT+TGG | 0.427558 | 3.4:-46019317 | MS.gene027218:CDS |
| GACAGAGAGGCGAGGCCCGC+CGG | 0.436040 | 3.4:+46020113 | None:intergenic |
| TGAAAACACAATGTTGTTGT+TGG | 0.440981 | 3.4:+46020293 | None:intergenic |
| GAAATCAACGGAAGCCAACT+TGG | 0.444733 | 3.4:+46020014 | None:intergenic |
| GATCGATTGATTTGAAACTA+AGG | 0.453659 | 3.4:+46020193 | None:intergenic |
| TGACCAATGAAGTCAATTCA+TGG | 0.453988 | 3.4:-46019648 | MS.gene027218:CDS |
| ATTCTTCCAGATGCGAAAGA+TGG | 0.456862 | 3.4:-46019331 | MS.gene027218:CDS |
| CGGAGGGAGCAGCGTCTTTG+AGG | 0.458037 | 3.4:+46020133 | None:intergenic |
| TCTATTTCACAAGTTAAAGT+AGG | 0.458733 | 3.4:-46019247 | MS.gene027218:CDS |
| CTCTGTCGCTAATGGTGTGT+GGG | 0.461547 | 3.4:-46020097 | MS.gene027218:CDS |
| CAACGGAAGCCAACTTGGCT+TGG | 0.461840 | 3.4:+46020019 | None:intergenic |
| CATCATCGCTGCCGGCTCTG+AGG | 0.467291 | 3.4:-46020244 | MS.gene027218:CDS |
| TTGTTTAGAGAAGAGATGTT+GGG | 0.479897 | 3.4:+46020317 | None:intergenic |
| TTTATCCAAGTAAACGAAGA+AGG | 0.480744 | 3.4:-46019061 | MS.gene027218:CDS |
| TCTCTTATTAGAAATAAGAA+TGG | 0.482752 | 3.4:+46018950 | None:intergenic |
| TAAAGTACATAGAGAATTGA+CGG | 0.488589 | 3.4:+46019353 | None:intergenic |
| ATCATCGCTGCCGGCTCTGA+GGG | 0.489637 | 3.4:-46020243 | MS.gene027218:CDS |
| GTCAAACCTCCGGGATAGAA+AGG | 0.493031 | 3.4:+46019145 | None:intergenic |
| CTCAAAGACGCTGCTCCCTC+CGG | 0.493872 | 3.4:-46020132 | MS.gene027218:CDS |
| TCTCAATGGCAGCTCAATCA+AGG | 0.494273 | 3.4:-46019470 | MS.gene027218:CDS |
| GTTAAGCTGTTGACCACCCC+TGG | 0.494814 | 3.4:+46019583 | None:intergenic |
| CTCTTATTAGAAATAAGAAT+GGG | 0.494945 | 3.4:+46018951 | None:intergenic |
| GGAATATGTTAGAAACAGAC+AGG | 0.497792 | 3.4:+46019092 | None:intergenic |
| ATTGTTTAGAGAAGAGATGT+TGG | 0.505542 | 3.4:+46020316 | None:intergenic |
| GGAAACGAGGCGAGAGGTGA+CGG | 0.513389 | 3.4:+46020161 | None:intergenic |
| TCCTTTCATGACCAGCATGA+AGG | 0.520615 | 3.4:-46019446 | MS.gene027218:CDS |
| CTGTGTGGTACCCTCAGAGC+CGG | 0.522370 | 3.4:+46020233 | None:intergenic |
| AAGACGCTGCTCCCTCCGGC+GGG | 0.522698 | 3.4:-46020128 | MS.gene027218:CDS |
| CTAATAAGAGAAGATTTGAC+CGG | 0.523302 | 3.4:-46018938 | MS.gene027218:CDS |
| AGAAACAGACAGGTCTTGAC+CGG | 0.523942 | 3.4:+46019102 | None:intergenic |
| TTGAAGTACTTTAAAACCAT+CGG | 0.525939 | 3.4:+46019405 | None:intergenic |
| AAAGACGCTGCTCCCTCCGG+CGG | 0.530193 | 3.4:-46020129 | MS.gene027218:CDS |
| CAATATATTCGTGCTTCCGA+TGG | 0.530199 | 3.4:-46019421 | MS.gene027218:CDS |
| TTCTGATTTCCAAGCCAAGT+TGG | 0.531191 | 3.4:-46020028 | MS.gene027218:CDS |
| ATTCTCAGCATCATCGCTGC+CGG | 0.533521 | 3.4:-46020252 | MS.gene027218:CDS |
| GAAGCTGCTGCTGCAACCTA+TGG | 0.534815 | 3.4:-46019034 | MS.gene027218:CDS |
| AGAAAGGTAAAACCACTCCT+AGG | 0.543661 | 3.4:+46019161 | None:intergenic |
| GCTAATGCCCTCTACTTCAA+AGG | 0.549267 | 3.4:-46019547 | MS.gene027218:CDS |
| TTCCGTTGATTTCCAGAACA+AGG | 0.549589 | 3.4:-46020004 | MS.gene027218:intron |
| CTATGGTACTTTGACAGCCA+TGG | 0.551444 | 3.4:-46019017 | MS.gene027218:CDS |
| ACCATACTCCTTTGAAGTAG+AGG | 0.553367 | 3.4:+46019539 | None:intergenic |
| CATGGCTGTCAAAGTACCAT+AGG | 0.558114 | 3.4:+46019018 | None:intergenic |
| AGGTGCTCAATCCTCTTGCT+GGG | 0.561778 | 3.4:-46018895 | MS.gene027218:CDS |
| GACCAATGAAGTCAATTCAT+GGG | 0.562924 | 3.4:-46019647 | MS.gene027218:CDS |
| CCAAATGAGATATTGAACCT+TGG | 0.563895 | 3.4:+46019211 | None:intergenic |
| ATTAAAGGAGTTATTCAACC+AGG | 0.564407 | 3.4:-46019601 | MS.gene027218:CDS |
| TCCTTCATGCTGGTCATGAA+AGG | 0.565992 | 3.4:+46019445 | None:intergenic |
| TGAAACTTCTGATATGCTGA+AGG | 0.568879 | 3.4:-46019185 | MS.gene027218:CDS |
| AATATATTGATCCTTCATGC+TGG | 0.580830 | 3.4:+46019435 | None:intergenic |
| TCTCTGTCGCTAATGGTGTG+TGG | 0.581743 | 3.4:-46020098 | MS.gene027218:CDS |
| CCATACTCCTTTGAAGTAGA+GGG | 0.583430 | 3.4:+46019540 | None:intergenic |
| TATGGTACTTTGACAGCCAT+GGG | 0.592161 | 3.4:-46019016 | MS.gene027218:CDS |
| TCTGATATGCTGAAGGACCT+AGG | 0.594866 | 3.4:-46019178 | MS.gene027218:CDS |
| TTAAAGGAGTTATTCAACCA+GGG | 0.595669 | 3.4:-46019600 | MS.gene027218:CDS |
| TAGTATGATTACCCAGCAAG+AGG | 0.598459 | 3.4:+46018884 | None:intergenic |
| CGGAGGTTTGACTAAAATGG+TGG | 0.598611 | 3.4:-46019134 | MS.gene027218:CDS |
| AGAGAGGCGAGGCCCGCCGG+AGG | 0.603320 | 3.4:+46020116 | None:intergenic |
| TCCAACAAAGAGGATTGTGC+CGG | 0.604900 | 3.4:+46018919 | None:intergenic |
| AGGAGTTATTCAACCAGGGG+TGG | 0.611289 | 3.4:-46019596 | MS.gene027218:CDS |
| GAGAGGCGAGGCCCGCCGGA+GGG | 0.613433 | 3.4:+46020117 | None:intergenic |
| TAAAGGAGTTATTCAACCAG+GGG | 0.617142 | 3.4:-46019599 | MS.gene027218:CDS |
| ACGAGGACGGCTTATACCCA+TGG | 0.623405 | 3.4:+46019000 | None:intergenic |
| TACCTTGTTCTGGAAATCAA+CGG | 0.624465 | 3.4:+46020002 | None:intergenic |
| TGATTGAGCTGCCATTGAGA+AGG | 0.630337 | 3.4:+46019473 | None:intergenic |
| AGCAGCGTCTTTGAGGATGA+CGG | 0.640464 | 3.4:+46020140 | None:intergenic |
| TGAGCACCTGTCCAACAAAG+AGG | 0.641694 | 3.4:+46018909 | None:intergenic |
| ACAAAGTCTAGTTGAGGACG+AGG | 0.644190 | 3.4:+46018983 | None:intergenic |
| CTTTGAGGATGACGGAAACG+AGG | 0.645095 | 3.4:+46020148 | None:intergenic |
| AGTCTAGTTGAGGACGAGGA+CGG | 0.649108 | 3.4:+46018987 | None:intergenic |
| AGAATAACTTGAATCGAGAG+TGG | 0.653506 | 3.4:+46020270 | None:intergenic |
| TAGGTGACTTCAGAATTCCA+AGG | 0.662092 | 3.4:-46019228 | MS.gene027218:CDS |
| TTATTAGAAATAAGAATGGG+TGG | 0.662615 | 3.4:+46018954 | None:intergenic |
| GATGACGGAAACGAGGCGAG+AGG | 0.671538 | 3.4:+46020155 | None:intergenic |
| GTGAAATAGAATGAAGCTTG+TGG | 0.693520 | 3.4:+46019260 | None:intergenic |
| TATGCTGAAGGACCTAGGAG+TGG | 0.695951 | 3.4:-46019173 | MS.gene027218:CDS |
| TCAGCTACAAAGTCTAGTTG+AGG | 0.698934 | 3.4:+46018977 | None:intergenic |
| CCATTAGCGACAGAGAGGCG+AGG | 0.701044 | 3.4:+46020105 | None:intergenic |
| ATCGAGAAGTTGTTGCTGTG+TGG | 0.709742 | 3.4:+46020218 | None:intergenic |
| ACACACCATTAGCGACAGAG+AGG | 0.711468 | 3.4:+46020100 | None:intergenic |
| GGGTGATCATTGATTCACGA+CGG | 0.714537 | 3.4:+46020364 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAGAAAATAAACAATTA+AGG | + | chr3.4:46019315-46019334 | None:intergenic | 15.0% |
| !! | ATACAAGAAAATATAAGATT+CGG | + | chr3.4:46019401-46019420 | None:intergenic | 15.0% |
| !! | TATTTAATTGCTAAATTAGT+TGG | + | chr3.4:46019523-46019542 | None:intergenic | 15.0% |
| !! | CTCTTATTAGAAATAAGAAT+GGG | + | chr3.4:46020316-46020335 | None:intergenic | 20.0% |
| !! | GAAATTTGATATGAATTGTT+AGG | - | chr3.4:46019584-46019603 | MS.gene027218:CDS | 20.0% |
| !! | TACAAGAAAATATAAGATTC+GGG | + | chr3.4:46019400-46019419 | None:intergenic | 20.0% |
| !! | TCTCTTATTAGAAATAAGAA+TGG | + | chr3.4:46020317-46020336 | None:intergenic | 20.0% |
| !! | TTGGATGTTAAAAAACAATA+AGG | + | chr3.4:46019504-46019523 | None:intergenic | 20.0% |
| !!! | GTAATATGTAATGTGTTTTA+GGG | - | chr3.4:46019286-46019305 | MS.gene027218:CDS | 20.0% |
| !!! | GTTCTATTTTTGATTTCATT+AGG | - | chr3.4:46019335-46019354 | MS.gene027218:CDS | 20.0% |
| !!! | TACTGTTTTCTATCAAATTA+GGG | - | chr3.4:46019372-46019391 | MS.gene027218:CDS | 20.0% |
| !!! | TGTAATATGTAATGTGTTTT+AGG | - | chr3.4:46019285-46019304 | MS.gene027218:CDS | 20.0% |
| !!! | TTACTGTTTTCTATCAAATT+AGG | - | chr3.4:46019371-46019390 | MS.gene027218:CDS | 20.0% |
| ! | GTTGAATAACTCCTTTAATA+AGG | + | chr3.4:46019662-46019681 | None:intergenic | 25.0% |
| ! | TAAAGTACATAGAGAATTGA+CGG | + | chr3.4:46019914-46019933 | None:intergenic | 25.0% |
| ! | TAATAATGGCCTAGTTTATA+AGG | + | chr3.4:46019888-46019907 | None:intergenic | 25.0% |
| ! | TAGAAAACAGTAAACAAACA+TGG | + | chr3.4:46019364-46019383 | None:intergenic | 25.0% |
| ! | TCTATTTCACAAGTTAAAGT+AGG | - | chr3.4:46020017-46020036 | MS.gene027218:CDS | 25.0% |
| ! | TTATTAGAAATAAGAATGGG+TGG | + | chr3.4:46020313-46020332 | None:intergenic | 25.0% |
| !! | TTACTTGGATAAAAGACTTT+TGG | + | chr3.4:46020196-46020215 | None:intergenic | 25.0% |
| !!! | TTGAAGTACTTTAAAACCAT+CGG | + | chr3.4:46019862-46019881 | None:intergenic | 25.0% |
| AATATATTGATCCTTCATGC+TGG | + | chr3.4:46019832-46019851 | None:intergenic | 30.0% | |
| ACATATTACATACCTTGTTC+TGG | + | chr3.4:46019275-46019294 | None:intergenic | 30.0% | |
| AGAATTGACGGTCATAATAA+TGG | + | chr3.4:46019902-46019921 | None:intergenic | 30.0% | |
| ATTAAAGGAGTTATTCAACC+AGG | - | chr3.4:46019663-46019682 | MS.gene027218:intron | 30.0% | |
| ATTGTTTAGAGAAGAGATGT+TGG | + | chr3.4:46018951-46018970 | None:intergenic | 30.0% | |
| CAAGGTTCAATATCTCATTT+GGG | - | chr3.4:46020054-46020073 | MS.gene027218:CDS | 30.0% | |
| CTAATAAGAGAAGATTTGAC+CGG | - | chr3.4:46020326-46020345 | MS.gene027218:CDS | 30.0% | |
| CTTCAACTTCCTTATAAACT+AGG | - | chr3.4:46019876-46019895 | MS.gene027218:intron | 30.0% | |
| GAAAGAAGTTTGAAGAGAAA+GGG | + | chr3.4:46019202-46019221 | None:intergenic | 30.0% | |
| TGAAAACACAATGTTGTTGT+TGG | + | chr3.4:46018974-46018993 | None:intergenic | 30.0% | |
| TTAAAGGAGTTATTCAACCA+GGG | - | chr3.4:46019664-46019683 | MS.gene027218:intron | 30.0% | |
| TTGTTTAGAGAAGAGATGTT+GGG | + | chr3.4:46018950-46018969 | None:intergenic | 30.0% | |
| TTTATCCAAGTAAACGAAGA+AGG | - | chr3.4:46020203-46020222 | MS.gene027218:CDS | 30.0% | |
| ! | AATCAGAAGAAACGATCTTT+TGG | + | chr3.4:46019224-46019243 | None:intergenic | 30.0% |
| ! | TATGATTTTCACCTTCTCAA+TGG | - | chr3.4:46019780-46019799 | MS.gene027218:intron | 30.0% |
| !! | GATCGATTGATTTGAAACTA+AGG | + | chr3.4:46019074-46019093 | None:intergenic | 30.0% |
| AGAATAACTTGAATCGAGAG+TGG | + | chr3.4:46018997-46019016 | None:intergenic | 35.0% | |
| CCAAATGAGATATTGAACCT+TGG | + | chr3.4:46020056-46020075 | None:intergenic | 35.0% | |
| CCAAGGTTCAATATCTCATT+TGG | - | chr3.4:46020053-46020072 | MS.gene027218:CDS | 35.0% | |
| GACCAATGAAGTCAATTCAT+GGG | - | chr3.4:46019617-46019636 | MS.gene027218:CDS | 35.0% | |
| GGAAAGAAGTTTGAAGAGAA+AGG | + | chr3.4:46019203-46019222 | None:intergenic | 35.0% | |
| GGAATATGTTAGAAACAGAC+AGG | + | chr3.4:46020175-46020194 | None:intergenic | 35.0% | |
| GTGAAATAGAATGAAGCTTG+TGG | + | chr3.4:46020007-46020026 | None:intergenic | 35.0% | |
| TAAAGGAGTTATTCAACCAG+GGG | - | chr3.4:46019665-46019684 | MS.gene027218:intron | 35.0% | |
| TGAAACTTCTGATATGCTGA+AGG | - | chr3.4:46020079-46020098 | MS.gene027218:CDS | 35.0% | |
| TGACCAATGAAGTCAATTCA+TGG | - | chr3.4:46019616-46019635 | MS.gene027218:CDS | 35.0% | |
| ! | AACCCATGAATTGACTTCAT+TGG | + | chr3.4:46019622-46019641 | None:intergenic | 35.0% |
| ! | TGGGTTGAAAAAGAGACAAA+TGG | - | chr3.4:46019636-46019655 | MS.gene027218:CDS | 35.0% |
| !! | GAGACAAATGGCCTTATTAA+AGG | - | chr3.4:46019648-46019667 | MS.gene027218:CDS | 35.0% |
| !! | TACCTTGTTCTGGAAATCAA+CGG | + | chr3.4:46019265-46019284 | None:intergenic | 35.0% |
| !! | TTTTGAGTGAAACGTTGGTT+CGG | + | chr3.4:46018924-46018943 | None:intergenic | 35.0% |
| AGAAAGGTAAAACCACTCCT+AGG | + | chr3.4:46020106-46020125 | None:intergenic | 40.0% | |
| ATTCTTCCAGATGCGAAAGA+TGG | - | chr3.4:46019933-46019952 | MS.gene027218:intron | 40.0% | |
| CAATATATTCGTGCTTCCGA+TGG | - | chr3.4:46019843-46019862 | MS.gene027218:intron | 40.0% | |
| TAGGTGACTTCAGAATTCCA+AGG | - | chr3.4:46020036-46020055 | MS.gene027218:CDS | 40.0% | |
| TATGGTACTTTGACAGCCAT+GGG | - | chr3.4:46020248-46020267 | MS.gene027218:CDS | 40.0% | |
| TCAGCTACAAAGTCTAGTTG+AGG | + | chr3.4:46020290-46020309 | None:intergenic | 40.0% | |
| TGTGTGTGTGAGTTAAGTCA+AGG | + | chr3.4:46019455-46019474 | None:intergenic | 40.0% | |
| TTCTGATTTCCAAGCCAAGT+TGG | - | chr3.4:46019236-46019255 | MS.gene027218:CDS | 40.0% | |
| ! | GAAAGATGGACTCTCAGATT+TGG | - | chr3.4:46019947-46019966 | MS.gene027218:intron | 40.0% |
| !! | ACCATACTCCTTTGAAGTAG+AGG | + | chr3.4:46019728-46019747 | None:intergenic | 40.0% |
| !! | ACCATTTTAGTCAAACCTCC+GGG | + | chr3.4:46020131-46020150 | None:intergenic | 40.0% |
| !! | CACCATTTTAGTCAAACCTC+CGG | + | chr3.4:46020132-46020151 | None:intergenic | 40.0% |
| !! | CCATACTCCTTTGAAGTAGA+GGG | + | chr3.4:46019727-46019746 | None:intergenic | 40.0% |
| !! | GTGGTTTTACCTTTCTATCC+CGG | - | chr3.4:46020110-46020129 | MS.gene027218:CDS | 40.0% |
| !! | TTCCGTTGATTTCCAGAACA+AGG | - | chr3.4:46019260-46019279 | MS.gene027218:CDS | 40.0% |
| !! | TTTGAGTGAAACGTTGGTTC+GGG | + | chr3.4:46018923-46018942 | None:intergenic | 40.0% |
| ACAAAGTCTAGTTGAGGACG+AGG | + | chr3.4:46020284-46020303 | None:intergenic | 45.0% | |
| AGAAACAGACAGGTCTTGAC+CGG | + | chr3.4:46020165-46020184 | None:intergenic | 45.0% | |
| ATCGAGAAGTTGTTGCTGTG+TGG | + | chr3.4:46019049-46019068 | None:intergenic | 45.0% | |
| CACAATCCTCTTTGTTGGAC+AGG | - | chr3.4:46020349-46020368 | MS.gene027218:CDS | 45.0% | |
| CAGTGCCTTCTTCGTTTACT+TGG | + | chr3.4:46020211-46020230 | None:intergenic | 45.0% | |
| CCCTCTACTTCAAAGGAGTA+TGG | - | chr3.4:46019724-46019743 | MS.gene027218:intron | 45.0% | |
| CGGAGGTTTGACTAAAATGG+TGG | - | chr3.4:46020130-46020149 | MS.gene027218:CDS | 45.0% | |
| CTATGGTACTTTGACAGCCA+TGG | - | chr3.4:46020247-46020266 | MS.gene027218:CDS | 45.0% | |
| GAAATCAACGGAAGCCAACT+TGG | + | chr3.4:46019253-46019272 | None:intergenic | 45.0% | |
| GCTAATGCCCTCTACTTCAA+AGG | - | chr3.4:46019717-46019736 | MS.gene027218:intron | 45.0% | |
| TCCAACAAAGAGGATTGTGC+CGG | + | chr3.4:46020348-46020367 | None:intergenic | 45.0% | |
| TCCCGGAGGTTTGACTAAAA+TGG | - | chr3.4:46020127-46020146 | MS.gene027218:CDS | 45.0% | |
| TCCTTCATGCTGGTCATGAA+AGG | + | chr3.4:46019822-46019841 | None:intergenic | 45.0% | |
| TCCTTTCATGACCAGCATGA+AGG | - | chr3.4:46019818-46019837 | MS.gene027218:intron | 45.0% | |
| TCTCAATGGCAGCTCAATCA+AGG | - | chr3.4:46019794-46019813 | MS.gene027218:intron | 45.0% | |
| TCTGATATGCTGAAGGACCT+AGG | - | chr3.4:46020086-46020105 | MS.gene027218:CDS | 45.0% | |
| TGATTGAGCTGCCATTGAGA+AGG | + | chr3.4:46019794-46019813 | None:intergenic | 45.0% | |
| ! | CATGGCTGTCAAAGTACCAT+AGG | + | chr3.4:46020249-46020268 | None:intergenic | 45.0% |
| ! | GTTTTACCTTTCTATCCCGG+AGG | - | chr3.4:46020113-46020132 | MS.gene027218:CDS | 45.0% |
| !! | AAAATGGTGGACTCACTTGC+CGG | - | chr3.4:46020143-46020162 | MS.gene027218:CDS | 45.0% |
| !! | GGCGATTTTGAGTGAAACGT+TGG | + | chr3.4:46018929-46018948 | None:intergenic | 45.0% |
| !! | GGGTGATCATTGATTCACGA+CGG | + | chr3.4:46018903-46018922 | None:intergenic | 45.0% |
| ACACACCATTAGCGACAGAG+AGG | + | chr3.4:46019167-46019186 | None:intergenic | 50.0% | |
| ACCGGCACAATCCTCTTTGT+TGG | - | chr3.4:46020344-46020363 | MS.gene027218:CDS | 50.0% | |
| AGGAGTTATTCAACCAGGGG+TGG | - | chr3.4:46019668-46019687 | MS.gene027218:intron | 50.0% | |
| AGGTGCTCAATCCTCTTGCT+GGG | - | chr3.4:46020369-46020388 | MS.gene027218:CDS | 50.0% | |
| AGTCTAGTTGAGGACGAGGA+CGG | + | chr3.4:46020280-46020299 | None:intergenic | 50.0% | |
| ATTCTCAGCATCATCGCTGC+CGG | - | chr3.4:46019012-46019031 | MS.gene027218:CDS | 50.0% | |
| CTCTGTCGCTAATGGTGTGT+GGG | - | chr3.4:46019167-46019186 | MS.gene027218:CDS | 50.0% | |
| GAGAGTCCATCTTTCGCATC+TGG | + | chr3.4:46019942-46019961 | None:intergenic | 50.0% | |
| GTCAAACCTCCGGGATAGAA+AGG | + | chr3.4:46020122-46020141 | None:intergenic | 50.0% | |
| TATGCTGAAGGACCTAGGAG+TGG | - | chr3.4:46020091-46020110 | MS.gene027218:CDS | 50.0% | |
| TCTCTGTCGCTAATGGTGTG+TGG | - | chr3.4:46019166-46019185 | MS.gene027218:CDS | 50.0% | |
| TGAGCACCTGTCCAACAAAG+AGG | + | chr3.4:46020358-46020377 | None:intergenic | 50.0% | |
| ! | AGCAGCGTCTTTGAGGATGA+CGG | + | chr3.4:46019127-46019146 | None:intergenic | 50.0% |
| !! | CTTTGAGGATGACGGAAACG+AGG | + | chr3.4:46019119-46019138 | None:intergenic | 50.0% |
| ACGAGGACGGCTTATACCCA+TGG | + | chr3.4:46020267-46020286 | None:intergenic | 55.0% | |
| CAACGGAAGCCAACTTGGCT+TGG | + | chr3.4:46019248-46019267 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | - | chr3.4:46020368-46020387 | MS.gene027218:CDS | 55.0% | |
| GAAGCTGCTGCTGCAACCTA+TGG | - | chr3.4:46020230-46020249 | MS.gene027218:CDS | 55.0% | |
| GTTAAGCTGTTGACCACCCC+TGG | + | chr3.4:46019684-46019703 | None:intergenic | 55.0% | |
| CCATTAGCGACAGAGAGGCG+AGG | + | chr3.4:46019162-46019181 | None:intergenic | 60.0% | |
| CCTCGCCTCTCTGTCGCTAA+TGG | - | chr3.4:46019159-46019178 | MS.gene027218:CDS | 60.0% | |
| CTCAAAGACGCTGCTCCCTC+CGG | - | chr3.4:46019132-46019151 | MS.gene027218:CDS | 60.0% | |
| CTGTGTGGTACCCTCAGAGC+CGG | + | chr3.4:46019034-46019053 | None:intergenic | 60.0% | |
| GGAAACGAGGCGAGAGGTGA+CGG | + | chr3.4:46019106-46019125 | None:intergenic | 60.0% | |
| !! | ATCATCGCTGCCGGCTCTGA+GGG | - | chr3.4:46019021-46019040 | MS.gene027218:CDS | 60.0% |
| !! | GATGACGGAAACGAGGCGAG+AGG | + | chr3.4:46019112-46019131 | None:intergenic | 60.0% |
| AAAGACGCTGCTCCCTCCGG+CGG | - | chr3.4:46019135-46019154 | MS.gene027218:CDS | 65.0% | |
| CGGAGGGAGCAGCGTCTTTG+AGG | + | chr3.4:46019134-46019153 | None:intergenic | 65.0% | |
| !! | CATCATCGCTGCCGGCTCTG+AGG | - | chr3.4:46019020-46019039 | MS.gene027218:CDS | 65.0% |
| AAGACGCTGCTCCCTCCGGC+GGG | - | chr3.4:46019136-46019155 | MS.gene027218:CDS | 70.0% | |
| GACAGAGAGGCGAGGCCCGC+CGG | + | chr3.4:46019154-46019173 | None:intergenic | 75.0% | |
| ! | AGAGAGGCGAGGCCCGCCGG+AGG | + | chr3.4:46019151-46019170 | None:intergenic | 80.0% |
| ! | GAGAGGCGAGGCCCGCCGGA+GGG | + | chr3.4:46019150-46019169 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 46018892 | 46020394 | 46018892 | ID=MS.gene027218 |
| chr3.4 | mRNA | 46018892 | 46020394 | 46018892 | ID=MS.gene027218.t1;Parent=MS.gene027218 |
| chr3.4 | exon | 46020005 | 46020394 | 46020005 | ID=MS.gene027218.t1.exon1;Parent=MS.gene027218.t1 |
| chr3.4 | CDS | 46020005 | 46020394 | 46020005 | ID=cds.MS.gene027218.t1;Parent=MS.gene027218.t1 |
| chr3.4 | exon | 46018892 | 46019680 | 46018892 | ID=MS.gene027218.t1.exon2;Parent=MS.gene027218.t1 |
| chr3.4 | CDS | 46018892 | 46019680 | 46018892 | ID=cds.MS.gene027218.t1;Parent=MS.gene027218.t1 |
| Gene Sequence |
| Protein sequence |
