Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027228.t1 | XP_013455531.1 | 88.8 | 392 | 40 | 3 | 1 | 392 | 1 | 388 | 5.30E-187 | 663.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027228.t1 | Q9S7T8 | 53.5 | 389 | 176 | 5 | 5 | 390 | 4 | 390 | 4.5e-110 | 399.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027228.t1 | A0A072UIQ8 | 88.8 | 392 | 40 | 3 | 1 | 392 | 1 | 388 | 3.8e-187 | 663.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene031697 | MS.gene027228 | PPI |
| MS.gene065945 | MS.gene027228 | PPI |
| MS.gene027228 | MS.gene045585 | PPI |
| MS.gene006610 | MS.gene027228 | PPI |
| MS.gene027228 | MS.gene065944 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027228.t1 | MTR_4g045707 | 88.776 | 392 | 40 | 3 | 1 | 392 | 1 | 388 | 0.0 | 691 |
| MS.gene027228.t1 | MTR_3g101010 | 72.634 | 391 | 99 | 4 | 5 | 392 | 4 | 389 | 0.0 | 531 |
| MS.gene027228.t1 | MTR_3g018740 | 69.251 | 387 | 114 | 4 | 5 | 390 | 4 | 386 | 0.0 | 517 |
| MS.gene027228.t1 | MTR_3g048000 | 66.406 | 384 | 108 | 2 | 7 | 390 | 28 | 390 | 0.0 | 512 |
| MS.gene027228.t1 | MTR_8g027395 | 66.150 | 387 | 125 | 3 | 6 | 392 | 17 | 397 | 3.84e-176 | 496 |
| MS.gene027228.t1 | MTR_3g101030 | 66.180 | 411 | 112 | 10 | 4 | 392 | 24 | 429 | 6.16e-175 | 494 |
| MS.gene027228.t1 | MTR_2g046030 | 64.872 | 390 | 126 | 5 | 5 | 390 | 4 | 386 | 1.39e-170 | 482 |
| MS.gene027228.t1 | MTR_2g045980 | 65.385 | 390 | 124 | 5 | 5 | 390 | 4 | 386 | 2.93e-169 | 478 |
| MS.gene027228.t1 | MTR_8g461160 | 63.659 | 399 | 129 | 6 | 1 | 390 | 1 | 392 | 7.59e-167 | 473 |
| MS.gene027228.t1 | MTR_3g101180 | 61.905 | 399 | 142 | 5 | 1 | 390 | 1 | 398 | 1.48e-166 | 472 |
| MS.gene027228.t1 | MTR_4g088525 | 65.940 | 367 | 119 | 4 | 25 | 391 | 8 | 368 | 6.13e-166 | 469 |
| MS.gene027228.t1 | MTR_7g050750 | 62.500 | 392 | 141 | 4 | 5 | 392 | 7 | 396 | 2.04e-165 | 469 |
| MS.gene027228.t1 | MTR_8g027420 | 63.049 | 387 | 126 | 4 | 6 | 392 | 6 | 375 | 1.75e-164 | 466 |
| MS.gene027228.t1 | MTR_7g451890 | 61.637 | 391 | 144 | 4 | 6 | 392 | 14 | 402 | 1.95e-163 | 464 |
| MS.gene027228.t1 | MTR_7g047780 | 62.148 | 391 | 142 | 4 | 6 | 392 | 14 | 402 | 7.74e-162 | 460 |
| MS.gene027228.t1 | MTR_3g101130 | 60.354 | 396 | 146 | 6 | 1 | 392 | 1 | 389 | 7.15e-158 | 450 |
| MS.gene027228.t1 | MTR_7g050810 | 60.560 | 393 | 145 | 5 | 6 | 392 | 16 | 404 | 2.87e-156 | 446 |
| MS.gene027228.t1 | MTR_4g088535 | 60.938 | 384 | 128 | 5 | 4 | 387 | 5 | 366 | 4.23e-154 | 440 |
| MS.gene027228.t1 | MTR_3g101050 | 61.965 | 397 | 120 | 8 | 3 | 391 | 24 | 397 | 4.37e-152 | 435 |
| MS.gene027228.t1 | MTR_3g015620 | 59.897 | 389 | 126 | 6 | 6 | 390 | 16 | 378 | 1.01e-141 | 408 |
| MS.gene027228.t1 | MTR_3g102920 | 57.545 | 391 | 124 | 7 | 4 | 390 | 56 | 408 | 1.04e-136 | 397 |
| MS.gene027228.t1 | MTR_3g101200 | 53.571 | 392 | 153 | 6 | 1 | 390 | 26 | 390 | 4.69e-127 | 371 |
| MS.gene027228.t1 | MTR_3g101190 | 51.781 | 365 | 111 | 4 | 28 | 392 | 13 | 312 | 3.29e-118 | 346 |
| MS.gene027228.t1 | MTR_3g100995 | 67.181 | 259 | 80 | 4 | 134 | 392 | 11 | 264 | 1.59e-116 | 340 |
| MS.gene027228.t1 | MTR_3g018747 | 65.267 | 262 | 73 | 5 | 130 | 390 | 9 | 253 | 2.66e-108 | 318 |
| MS.gene027228.t1 | MTR_3g100010 | 55.414 | 314 | 90 | 4 | 31 | 311 | 42 | 338 | 1.40e-104 | 312 |
| MS.gene027228.t1 | MTR_7g050830 | 53.165 | 316 | 113 | 5 | 6 | 299 | 16 | 318 | 1.06e-101 | 304 |
| MS.gene027228.t1 | MTR_3g015760 | 60.741 | 270 | 100 | 4 | 122 | 390 | 15 | 279 | 1.58e-98 | 295 |
| MS.gene027228.t1 | MTR_2g046410 | 67.308 | 208 | 60 | 4 | 187 | 390 | 144 | 347 | 1.48e-88 | 271 |
| MS.gene027228.t1 | MTR_3g100520 | 61.883 | 223 | 70 | 3 | 171 | 392 | 1 | 209 | 1.58e-88 | 266 |
| MS.gene027228.t1 | MTR_3g111160 | 67.411 | 224 | 67 | 6 | 171 | 392 | 1 | 220 | 2.49e-88 | 266 |
| MS.gene027228.t1 | MTR_3g114310 | 40.759 | 395 | 213 | 9 | 11 | 392 | 24 | 410 | 2.69e-86 | 268 |
| MS.gene027228.t1 | MTR_3g099970 | 65.657 | 198 | 49 | 4 | 130 | 327 | 20 | 198 | 1.10e-81 | 249 |
| MS.gene027228.t1 | MTR_3g101110 | 52.697 | 241 | 82 | 6 | 130 | 366 | 68 | 280 | 6.67e-71 | 224 |
| MS.gene027228.t1 | MTR_3g111150 | 58.192 | 177 | 50 | 1 | 8 | 184 | 1 | 153 | 3.32e-62 | 197 |
| MS.gene027228.t1 | MTR_3g101150 | 65.190 | 158 | 50 | 3 | 237 | 390 | 1 | 157 | 3.48e-61 | 194 |
| MS.gene027228.t1 | MTR_3g101020 | 56.614 | 189 | 53 | 4 | 204 | 392 | 1 | 160 | 8.09e-58 | 186 |
| MS.gene027228.t1 | MTR_3g102870 | 61.783 | 157 | 58 | 2 | 193 | 349 | 1 | 155 | 2.14e-57 | 186 |
| MS.gene027228.t1 | MTR_3g100990 | 64.885 | 131 | 46 | 0 | 4 | 134 | 3 | 133 | 5.82e-48 | 170 |
| MS.gene027228.t1 | MTR_4g093550 | 66.667 | 84 | 28 | 0 | 6 | 89 | 6 | 89 | 4.08e-30 | 111 |
| MS.gene027228.t1 | MTR_3g101140 | 64.286 | 70 | 22 | 2 | 325 | 392 | 48 | 116 | 3.79e-20 | 85.5 |
| MS.gene027228.t1 | MTR_6g046890 | 57.143 | 84 | 33 | 1 | 14 | 97 | 7 | 87 | 7.22e-19 | 82.8 |
| MS.gene027228.t1 | MTR_3g101080 | 92.857 | 28 | 2 | 0 | 355 | 382 | 12 | 39 | 7.39e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027228.t1 | AT1G47710 | 53.470 | 389 | 176 | 5 | 5 | 390 | 4 | 390 | 8.97e-142 | 409 |
| MS.gene027228.t1 | AT1G47710 | 53.470 | 389 | 176 | 5 | 5 | 390 | 31 | 417 | 2.84e-141 | 409 |
| MS.gene027228.t1 | AT2G25240 | 48.082 | 391 | 183 | 9 | 6 | 389 | 5 | 382 | 1.96e-118 | 349 |
| MS.gene027228.t1 | AT3G45220 | 45.729 | 398 | 190 | 7 | 6 | 389 | 5 | 390 | 1.43e-117 | 347 |
| MS.gene027228.t1 | AT2G26390 | 45.387 | 401 | 183 | 11 | 6 | 389 | 5 | 386 | 2.96e-108 | 323 |
| MS.gene027228.t1 | AT1G62170 | 43.112 | 392 | 193 | 6 | 6 | 391 | 66 | 433 | 4.74e-101 | 306 |
| MS.gene027228.t1 | AT2G14540 | 44.987 | 389 | 187 | 7 | 5 | 389 | 32 | 397 | 1.06e-99 | 302 |
| MS.gene027228.t1 | AT1G64030 | 43.734 | 391 | 191 | 8 | 5 | 389 | 4 | 371 | 5.96e-99 | 300 |
| MS.gene027228.t1 | AT1G62170 | 39.765 | 425 | 193 | 7 | 6 | 391 | 66 | 466 | 4.59e-94 | 290 |
| MS.gene027228.t1 | AT2G35580 | 41.432 | 391 | 186 | 9 | 5 | 389 | 18 | 371 | 2.20e-89 | 275 |
| MS.gene027228.t1 | AT1G64010 | 49.772 | 219 | 86 | 5 | 171 | 389 | 1 | 195 | 1.95e-64 | 204 |
| MS.gene027228.t1 | AT1G64010 | 49.020 | 204 | 80 | 5 | 186 | 389 | 2 | 181 | 9.03e-57 | 184 |
| MS.gene027228.t1 | AT1G62160 | 39.901 | 203 | 94 | 5 | 193 | 391 | 42 | 220 | 1.29e-33 | 125 |
| MS.gene027228.t1 | AT1G63280 | 53.043 | 115 | 51 | 2 | 130 | 243 | 8 | 120 | 4.36e-32 | 117 |
| MS.gene027228.t1 | AT1G51330 | 50.893 | 112 | 51 | 3 | 129 | 237 | 30 | 140 | 2.84e-26 | 104 |
| MS.gene027228.t1 | AT1G64020 | 37.288 | 118 | 67 | 4 | 7 | 120 | 6 | 120 | 2.54e-16 | 75.1 |
Find 100 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGGTTCAATATCTCATT+TGG | 0.243686 | 3.4:-45902109 | MS.gene027228:CDS |
| GTTGAATAACTCCTTTAATA+AGG | 0.260616 | 3.4:+45902503 | None:intergenic |
| CAGTGCCTTCTTCGTTTACT+TGG | 0.271310 | 3.4:+45901954 | None:intergenic |
| GAGACAAATGGCCTTATTAA+AGG | 0.273581 | 3.4:-45902514 | MS.gene027228:CDS |
| TTACTTGGATAAAAGACTTT+TGG | 0.279450 | 3.4:+45901969 | None:intergenic |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.322054 | 3.4:-45901794 | MS.gene027228:CDS |
| ACATATTACATACCTTGTTC+TGG | 0.328120 | 3.4:+45902902 | None:intergenic |
| CCTCGCCTCTCTGTCGCTAA+TGG | 0.342690 | 3.4:-45903015 | MS.gene027228:CDS |
| TCCCGGAGGTTTGACTAAAA+TGG | 0.356212 | 3.4:-45902035 | MS.gene027228:CDS |
| TAATAATGGCCTAGTTTATA+AGG | 0.358860 | 3.4:+45902277 | None:intergenic |
| AGAATTGACGGTCATAATAA+TGG | 0.381177 | 3.4:+45902263 | None:intergenic |
| ACCGGCACAATCCTCTTTGT+TGG | 0.389726 | 3.4:-45901818 | MS.gene027228:CDS |
| AACCCATGAATTGACTTCAT+TGG | 0.393781 | 3.4:+45902543 | None:intergenic |
| CACAATCCTCTTTGTTGGAC+AGG | 0.397313 | 3.4:-45901813 | MS.gene027228:CDS |
| CAAGGTTCAATATCTCATTT+GGG | 0.405007 | 3.4:-45902108 | MS.gene027228:CDS |
| GAGAGTCCATCTTTCGCATC+TGG | 0.410672 | 3.4:+45902223 | None:intergenic |
| CTTCAACTTCCTTATAAACT+AGG | 0.412556 | 3.4:-45902286 | MS.gene027228:CDS |
| CCCTCTACTTCAAAGGAGTA+TGG | 0.413172 | 3.4:-45902438 | MS.gene027228:CDS |
| AGGTGACGGAGTTGAGTTGA+TGG | 0.415102 | 3.4:+45903085 | None:intergenic |
| AAAATGGTGGACTCACTTGC+CGG | 0.418030 | 3.4:-45902019 | MS.gene027228:CDS |
| GAAAGATGGACTCTCAGATT+TGG | 0.427558 | 3.4:-45902215 | MS.gene027228:CDS |
| GACAGAGAGGCGAGGCCCGC+CGG | 0.436040 | 3.4:+45903023 | None:intergenic |
| TGAAAACACAATGTTGTTGT+TGG | 0.440981 | 3.4:+45903203 | None:intergenic |
| GAAATCAACGGAAGCCAACT+TGG | 0.444733 | 3.4:+45902924 | None:intergenic |
| GATGGATTGATTTGAAACTA+AGG | 0.453964 | 3.4:+45903103 | None:intergenic |
| TGACCAATGAAGTCAATTCA+TGG | 0.453988 | 3.4:-45902546 | MS.gene027228:CDS |
| ATTCTTCCAGATGCGAAAGA+TGG | 0.456862 | 3.4:-45902229 | MS.gene027228:CDS |
| CGGAGGGAGCAGCGTCTTTG+AGG | 0.458037 | 3.4:+45903043 | None:intergenic |
| TCTATTTCACAAGTTAAAGT+AGG | 0.458733 | 3.4:-45902145 | MS.gene027228:CDS |
| CTCTGTCGCTAATGGTGTGT+GGG | 0.461547 | 3.4:-45903007 | MS.gene027228:CDS |
| CAACGGAAGCCAACTTGGCT+TGG | 0.461840 | 3.4:+45902929 | None:intergenic |
| CATCATCGCTGCCGGCTCTG+AGG | 0.467291 | 3.4:-45903154 | MS.gene027228:CDS |
| AGAAACAAACAGGTCTTGAC+CGG | 0.477188 | 3.4:+45902000 | None:intergenic |
| TTGTTTAGAGAAGAGATGTT+GGG | 0.479897 | 3.4:+45903227 | None:intergenic |
| TTTATCCAAGTAAACGAAGA+AGG | 0.480744 | 3.4:-45901959 | MS.gene027228:CDS |
| TCTCTTATTAGAAATAAGAA+TGG | 0.482752 | 3.4:+45901848 | None:intergenic |
| TAAAGTACATAGAGAATTGA+CGG | 0.488589 | 3.4:+45902251 | None:intergenic |
| ATCATCGCTGCCGGCTCTGA+GGG | 0.489637 | 3.4:-45903153 | MS.gene027228:CDS |
| GTCAAACCTCCGGGATAGAA+AGG | 0.493031 | 3.4:+45902043 | None:intergenic |
| CTCAAAGACGCTGCTCCCTC+CGG | 0.493872 | 3.4:-45903042 | MS.gene027228:CDS |
| TCTCAATGGCAGCTCAATCA+AGG | 0.494273 | 3.4:-45902368 | MS.gene027228:CDS |
| GTTAAGCTGTTGACCACCCC+TGG | 0.494814 | 3.4:+45902481 | None:intergenic |
| CTCTTATTAGAAATAAGAAT+GGG | 0.494945 | 3.4:+45901849 | None:intergenic |
| ATTGTTTAGAGAAGAGATGT+TGG | 0.505542 | 3.4:+45903226 | None:intergenic |
| GGAAACGAGGCGAGAGGTGA+CGG | 0.513389 | 3.4:+45903071 | None:intergenic |
| TCCTTTCATGACCAGCATGA+AGG | 0.520615 | 3.4:-45902344 | MS.gene027228:CDS |
| CTGTGTGGTACCCTCAGAGC+CGG | 0.522370 | 3.4:+45903143 | None:intergenic |
| AAGACGCTGCTCCCTCCGGC+GGG | 0.522698 | 3.4:-45903038 | MS.gene027228:CDS |
| CTAATAAGAGAAGATTTGAC+CGG | 0.523302 | 3.4:-45901836 | MS.gene027228:CDS |
| TTGAAGTACTTTAAAACCAT+CGG | 0.525939 | 3.4:+45902303 | None:intergenic |
| AAAGACGCTGCTCCCTCCGG+CGG | 0.530193 | 3.4:-45903039 | MS.gene027228:CDS |
| CAATATATTCGTGCTTCCGA+TGG | 0.530199 | 3.4:-45902319 | MS.gene027228:CDS |
| TTCTGATTTCCAAGCCAAGT+TGG | 0.531191 | 3.4:-45902938 | MS.gene027228:CDS |
| ATTCTCAGCATCATCGCTGC+CGG | 0.533521 | 3.4:-45903162 | MS.gene027228:CDS |
| GAAGCTGCTGCTGCAACCTA+TGG | 0.534815 | 3.4:-45901932 | MS.gene027228:CDS |
| AGAAAGGTAAAACCACTCCT+AGG | 0.543661 | 3.4:+45902059 | None:intergenic |
| GCTAATGCCCTCTACTTCAA+AGG | 0.549267 | 3.4:-45902445 | MS.gene027228:CDS |
| TTCCGTTGATTTCCAGAACA+AGG | 0.549589 | 3.4:-45902914 | MS.gene027228:intron |
| CTATGGTACTTTGACAGCCA+TGG | 0.551444 | 3.4:-45901915 | MS.gene027228:CDS |
| ACCATACTCCTTTGAAGTAG+AGG | 0.553367 | 3.4:+45902437 | None:intergenic |
| CATGGCTGTCAAAGTACCAT+AGG | 0.558114 | 3.4:+45901916 | None:intergenic |
| ACAAAGTCTAGTCGAGGACG+AGG | 0.560984 | 3.4:+45901881 | None:intergenic |
| AGGTGCTCAATCCTCTTGCT+GGG | 0.561778 | 3.4:-45901793 | MS.gene027228:CDS |
| GACCAATGAAGTCAATTCAT+GGG | 0.562924 | 3.4:-45902545 | MS.gene027228:CDS |
| CCAAATGAGATATTGAACCT+TGG | 0.563895 | 3.4:+45902109 | None:intergenic |
| ATTAAAGGAGTTATTCAACC+AGG | 0.564407 | 3.4:-45902499 | MS.gene027228:CDS |
| TCCTTCATGCTGGTCATGAA+AGG | 0.565992 | 3.4:+45902343 | None:intergenic |
| TGAAACTTCTGATATGCTGA+AGG | 0.568879 | 3.4:-45902083 | MS.gene027228:CDS |
| AATATATTGATCCTTCATGC+TGG | 0.580830 | 3.4:+45902333 | None:intergenic |
| TCTCTGTCGCTAATGGTGTG+TGG | 0.581743 | 3.4:-45903008 | MS.gene027228:CDS |
| CCATACTCCTTTGAAGTAGA+GGG | 0.583430 | 3.4:+45902438 | None:intergenic |
| TATGGTACTTTGACAGCCAT+GGG | 0.592161 | 3.4:-45901914 | MS.gene027228:CDS |
| TCTGATATGCTGAAGGACCT+AGG | 0.594866 | 3.4:-45902076 | MS.gene027228:CDS |
| TTAAAGGAGTTATTCAACCA+GGG | 0.595669 | 3.4:-45902498 | MS.gene027228:CDS |
| TAGTATGATTACCCAGCAAG+AGG | 0.598459 | 3.4:+45901782 | None:intergenic |
| CGGAGGTTTGACTAAAATGG+TGG | 0.598611 | 3.4:-45902032 | MS.gene027228:CDS |
| AGAGAGGCGAGGCCCGCCGG+AGG | 0.603320 | 3.4:+45903026 | None:intergenic |
| TCCAACAAAGAGGATTGTGC+CGG | 0.604900 | 3.4:+45901817 | None:intergenic |
| ACGAGGAGGGCTTATACCCA+TGG | 0.610987 | 3.4:+45901898 | None:intergenic |
| AGGAGTTATTCAACCAGGGG+TGG | 0.611289 | 3.4:-45902494 | MS.gene027228:CDS |
| GAGAGGCGAGGCCCGCCGGA+GGG | 0.613433 | 3.4:+45903027 | None:intergenic |
| TAAAGGAGTTATTCAACCAG+GGG | 0.617142 | 3.4:-45902497 | MS.gene027228:CDS |
| TACCTTGTTCTGGAAATCAA+CGG | 0.624465 | 3.4:+45902912 | None:intergenic |
| AGTCTAGTCGAGGACGAGGA+GGG | 0.627803 | 3.4:+45901885 | None:intergenic |
| TGATTGAGCTGCCATTGAGA+AGG | 0.630337 | 3.4:+45902371 | None:intergenic |
| AAGTCTAGTCGAGGACGAGG+AGG | 0.638727 | 3.4:+45901884 | None:intergenic |
| AGCAGCGTCTTTGAGGATGA+CGG | 0.640464 | 3.4:+45903050 | None:intergenic |
| TGAGCACCTGTCCAACAAAG+AGG | 0.641694 | 3.4:+45901807 | None:intergenic |
| CTTTGAGGATGACGGAAACG+AGG | 0.645095 | 3.4:+45903058 | None:intergenic |
| AGAATAACTTGAATCGAGAG+TGG | 0.653506 | 3.4:+45903180 | None:intergenic |
| GGTTGATCATTGATTCACGA+CGG | 0.660933 | 3.4:+45903274 | None:intergenic |
| TAGGTGACTTCAGAATTCCA+AGG | 0.662092 | 3.4:-45902126 | MS.gene027228:CDS |
| TTATTAGAAATAAGAATGGG+TGG | 0.662615 | 3.4:+45901852 | None:intergenic |
| GATGACGGAAACGAGGCGAG+AGG | 0.671538 | 3.4:+45903065 | None:intergenic |
| GTGAAATAGAATGAAGCTTG+TGG | 0.680859 | 3.4:+45902158 | None:intergenic |
| TCAGCTACAAAGTCTAGTCG+AGG | 0.689387 | 3.4:+45901875 | None:intergenic |
| TATGCTGAAGGACCTAGGAG+TGG | 0.695951 | 3.4:-45902071 | MS.gene027228:CDS |
| CCATTAGCGACAGAGAGGCG+AGG | 0.701044 | 3.4:+45903015 | None:intergenic |
| ATCGAGAAGTTGTTGCTGTG+TGG | 0.709742 | 3.4:+45903128 | None:intergenic |
| ACACACCATTAGCGACAGAG+AGG | 0.711468 | 3.4:+45903010 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAGAAAATAAACAATTA+AGG | + | chr3.4:45902213-45902232 | None:intergenic | 15.0% |
| !! | ATACAAGAAAATATAAGATT+CGG | + | chr3.4:45902299-45902318 | None:intergenic | 15.0% |
| !! | TATTTAATTGCTAAATTAGT+TGG | + | chr3.4:45902428-45902447 | None:intergenic | 15.0% |
| !! | CTCTTATTAGAAATAAGAAT+GGG | + | chr3.4:45903226-45903245 | None:intergenic | 20.0% |
| !! | GAAATTTGATATGAATTGTT+AGG | - | chr3.4:45902494-45902513 | MS.gene027228:CDS | 20.0% |
| !! | TACAAGAAAATATAAGATTC+GGG | + | chr3.4:45902298-45902317 | None:intergenic | 20.0% |
| !! | TCTCTTATTAGAAATAAGAA+TGG | + | chr3.4:45903227-45903246 | None:intergenic | 20.0% |
| !! | TTGGATGTTAAAAAACAATA+AGG | + | chr3.4:45902409-45902428 | None:intergenic | 20.0% |
| !!! | GTAATATGTAATGTGTTTTA+GGG | - | chr3.4:45902184-45902203 | MS.gene027228:CDS | 20.0% |
| !!! | GTTCTATTTTTGATTTCATT+AGG | - | chr3.4:45902233-45902252 | MS.gene027228:CDS | 20.0% |
| !!! | TACTGTTTTCTATCAAATTA+GGG | - | chr3.4:45902270-45902289 | MS.gene027228:CDS | 20.0% |
| !!! | TGTAATATGTAATGTGTTTT+AGG | - | chr3.4:45902183-45902202 | MS.gene027228:CDS | 20.0% |
| !!! | TTACTGTTTTCTATCAAATT+AGG | - | chr3.4:45902269-45902288 | MS.gene027228:CDS | 20.0% |
| ! | GTTGAATAACTCCTTTAATA+AGG | + | chr3.4:45902572-45902591 | None:intergenic | 25.0% |
| ! | TAAAGTACATAGAGAATTGA+CGG | + | chr3.4:45902824-45902843 | None:intergenic | 25.0% |
| ! | TAATAATGGCCTAGTTTATA+AGG | + | chr3.4:45902798-45902817 | None:intergenic | 25.0% |
| ! | TAGAAAACAGTAAACAAACA+TGG | + | chr3.4:45902262-45902281 | None:intergenic | 25.0% |
| ! | TCTATTTCACAAGTTAAAGT+AGG | - | chr3.4:45902927-45902946 | MS.gene027228:CDS | 25.0% |
| ! | TTATTAGAAATAAGAATGGG+TGG | + | chr3.4:45903223-45903242 | None:intergenic | 25.0% |
| !! | TTACTTGGATAAAAGACTTT+TGG | + | chr3.4:45903106-45903125 | None:intergenic | 25.0% |
| !!! | GGAATATTTTAGAAACAAAC+AGG | + | chr3.4:45903085-45903104 | None:intergenic | 25.0% |
| !!! | TTGAAGTACTTTAAAACCAT+CGG | + | chr3.4:45902772-45902791 | None:intergenic | 25.0% |
| AATATATTGATCCTTCATGC+TGG | + | chr3.4:45902742-45902761 | None:intergenic | 30.0% | |
| ACATATTACATACCTTGTTC+TGG | + | chr3.4:45902173-45902192 | None:intergenic | 30.0% | |
| AGAATTGACGGTCATAATAA+TGG | + | chr3.4:45902812-45902831 | None:intergenic | 30.0% | |
| ATTAAAGGAGTTATTCAACC+AGG | - | chr3.4:45902573-45902592 | MS.gene027228:intron | 30.0% | |
| ATTGTTTAGAGAAGAGATGT+TGG | + | chr3.4:45901849-45901868 | None:intergenic | 30.0% | |
| CAAGGTTCAATATCTCATTT+GGG | - | chr3.4:45902964-45902983 | MS.gene027228:CDS | 30.0% | |
| CTAATAAGAGAAGATTTGAC+CGG | - | chr3.4:45903236-45903255 | MS.gene027228:CDS | 30.0% | |
| CTTCAACTTCCTTATAAACT+AGG | - | chr3.4:45902786-45902805 | MS.gene027228:intron | 30.0% | |
| TGAAAACACAATGTTGTTGT+TGG | + | chr3.4:45901872-45901891 | None:intergenic | 30.0% | |
| TTAAAGGAGTTATTCAACCA+GGG | - | chr3.4:45902574-45902593 | MS.gene027228:intron | 30.0% | |
| TTGTTTAGAGAAGAGATGTT+GGG | + | chr3.4:45901848-45901867 | None:intergenic | 30.0% | |
| TTTATCCAAGTAAACGAAGA+AGG | - | chr3.4:45903113-45903132 | MS.gene027228:CDS | 30.0% | |
| ! | TATGATTTTCACCTTCTCAA+TGG | - | chr3.4:45902690-45902709 | MS.gene027228:intron | 30.0% |
| !! | GATGGATTGATTTGAAACTA+AGG | + | chr3.4:45901972-45901991 | None:intergenic | 30.0% |
| AGAATAACTTGAATCGAGAG+TGG | + | chr3.4:45901895-45901914 | None:intergenic | 35.0% | |
| CCAAATGAGATATTGAACCT+TGG | + | chr3.4:45902966-45902985 | None:intergenic | 35.0% | |
| CCAAGGTTCAATATCTCATT+TGG | - | chr3.4:45902963-45902982 | MS.gene027228:CDS | 35.0% | |
| GACCAATGAAGTCAATTCAT+GGG | - | chr3.4:45902527-45902546 | MS.gene027228:CDS | 35.0% | |
| GTGAAATAGAATGAAGCTTG+TGG | + | chr3.4:45902917-45902936 | None:intergenic | 35.0% | |
| TAAAGGAGTTATTCAACCAG+GGG | - | chr3.4:45902575-45902594 | MS.gene027228:intron | 35.0% | |
| TGAAACTTCTGATATGCTGA+AGG | - | chr3.4:45902989-45903008 | MS.gene027228:CDS | 35.0% | |
| TGACCAATGAAGTCAATTCA+TGG | - | chr3.4:45902526-45902545 | MS.gene027228:CDS | 35.0% | |
| ! | AACCCATGAATTGACTTCAT+TGG | + | chr3.4:45902532-45902551 | None:intergenic | 35.0% |
| ! | TGGGTTGAAAAAGAGACAAA+TGG | - | chr3.4:45902546-45902565 | MS.gene027228:CDS | 35.0% |
| !! | GAGACAAATGGCCTTATTAA+AGG | - | chr3.4:45902558-45902577 | MS.gene027228:CDS | 35.0% |
| !! | TACCTTGTTCTGGAAATCAA+CGG | + | chr3.4:45902163-45902182 | None:intergenic | 35.0% |
| !! | TTTTGAGTGAAACGTTGGTT+TGG | + | chr3.4:45901822-45901841 | None:intergenic | 35.0% |
| AGAAACAAACAGGTCTTGAC+CGG | + | chr3.4:45903075-45903094 | None:intergenic | 40.0% | |
| AGAAAGGTAAAACCACTCCT+AGG | + | chr3.4:45903016-45903035 | None:intergenic | 40.0% | |
| ATTCTTCCAGATGCGAAAGA+TGG | - | chr3.4:45902843-45902862 | MS.gene027228:intron | 40.0% | |
| CAATATATTCGTGCTTCCGA+TGG | - | chr3.4:45902753-45902772 | MS.gene027228:intron | 40.0% | |
| GTGTGAGAGTTAAGTCAACA+TGG | + | chr3.4:45902353-45902372 | None:intergenic | 40.0% | |
| TAGGTGACTTCAGAATTCCA+AGG | - | chr3.4:45902946-45902965 | MS.gene027228:CDS | 40.0% | |
| TATGGTACTTTGACAGCCAT+GGG | - | chr3.4:45903158-45903177 | MS.gene027228:CDS | 40.0% | |
| TTCTGATTTCCAAGCCAAGT+TGG | - | chr3.4:45902134-45902153 | MS.gene027228:CDS | 40.0% | |
| ! | GAAAGATGGACTCTCAGATT+TGG | - | chr3.4:45902857-45902876 | MS.gene027228:intron | 40.0% |
| !! | ACCATACTCCTTTGAAGTAG+AGG | + | chr3.4:45902638-45902657 | None:intergenic | 40.0% |
| !! | ACCATTTTAGTCAAACCTCC+GGG | + | chr3.4:45903041-45903060 | None:intergenic | 40.0% |
| !! | CACCATTTTAGTCAAACCTC+CGG | + | chr3.4:45903042-45903061 | None:intergenic | 40.0% |
| !! | CCATACTCCTTTGAAGTAGA+GGG | + | chr3.4:45902637-45902656 | None:intergenic | 40.0% |
| !! | GGTTGATCATTGATTCACGA+CGG | + | chr3.4:45901801-45901820 | None:intergenic | 40.0% |
| !! | GTGGTTTTACCTTTCTATCC+CGG | - | chr3.4:45903020-45903039 | MS.gene027228:CDS | 40.0% |
| !! | TTCCGTTGATTTCCAGAACA+AGG | - | chr3.4:45902158-45902177 | MS.gene027228:CDS | 40.0% |
| ATCGAGAAGTTGTTGCTGTG+TGG | + | chr3.4:45901947-45901966 | None:intergenic | 45.0% | |
| CACAATCCTCTTTGTTGGAC+AGG | - | chr3.4:45903259-45903278 | MS.gene027228:CDS | 45.0% | |
| CAGTGCCTTCTTCGTTTACT+TGG | + | chr3.4:45903121-45903140 | None:intergenic | 45.0% | |
| CCCTCTACTTCAAAGGAGTA+TGG | - | chr3.4:45902634-45902653 | MS.gene027228:intron | 45.0% | |
| CGGAGGTTTGACTAAAATGG+TGG | - | chr3.4:45903040-45903059 | MS.gene027228:CDS | 45.0% | |
| CTATGGTACTTTGACAGCCA+TGG | - | chr3.4:45903157-45903176 | MS.gene027228:CDS | 45.0% | |
| GAAATCAACGGAAGCCAACT+TGG | + | chr3.4:45902151-45902170 | None:intergenic | 45.0% | |
| GCTAATGCCCTCTACTTCAA+AGG | - | chr3.4:45902627-45902646 | MS.gene027228:intron | 45.0% | |
| TCAGCTACAAAGTCTAGTCG+AGG | + | chr3.4:45903200-45903219 | None:intergenic | 45.0% | |
| TCCAACAAAGAGGATTGTGC+CGG | + | chr3.4:45903258-45903277 | None:intergenic | 45.0% | |
| TCCCGGAGGTTTGACTAAAA+TGG | - | chr3.4:45903037-45903056 | MS.gene027228:CDS | 45.0% | |
| TCCTTCATGCTGGTCATGAA+AGG | + | chr3.4:45902732-45902751 | None:intergenic | 45.0% | |
| TCCTTTCATGACCAGCATGA+AGG | - | chr3.4:45902728-45902747 | MS.gene027228:intron | 45.0% | |
| TCTCAATGGCAGCTCAATCA+AGG | - | chr3.4:45902704-45902723 | MS.gene027228:intron | 45.0% | |
| TCTGATATGCTGAAGGACCT+AGG | - | chr3.4:45902996-45903015 | MS.gene027228:CDS | 45.0% | |
| TGATTGAGCTGCCATTGAGA+AGG | + | chr3.4:45902704-45902723 | None:intergenic | 45.0% | |
| ! | CATGGCTGTCAAAGTACCAT+AGG | + | chr3.4:45903159-45903178 | None:intergenic | 45.0% |
| ! | GTTTTACCTTTCTATCCCGG+AGG | - | chr3.4:45903023-45903042 | MS.gene027228:CDS | 45.0% |
| !! | AAAATGGTGGACTCACTTGC+CGG | - | chr3.4:45903053-45903072 | MS.gene027228:CDS | 45.0% |
| !! | GGCGATTTTGAGTGAAACGT+TGG | + | chr3.4:45901827-45901846 | None:intergenic | 45.0% |
| ACAAAGTCTAGTCGAGGACG+AGG | + | chr3.4:45903194-45903213 | None:intergenic | 50.0% | |
| ACACACCATTAGCGACAGAG+AGG | + | chr3.4:45902065-45902084 | None:intergenic | 50.0% | |
| ACCGGCACAATCCTCTTTGT+TGG | - | chr3.4:45903254-45903273 | MS.gene027228:CDS | 50.0% | |
| AGGAGTTATTCAACCAGGGG+TGG | - | chr3.4:45902578-45902597 | MS.gene027228:intron | 50.0% | |
| AGGTGCTCAATCCTCTTGCT+GGG | - | chr3.4:45903279-45903298 | MS.gene027228:CDS | 50.0% | |
| ATTCTCAGCATCATCGCTGC+CGG | - | chr3.4:45901910-45901929 | MS.gene027228:CDS | 50.0% | |
| CTCTGTCGCTAATGGTGTGT+GGG | - | chr3.4:45902065-45902084 | MS.gene027228:CDS | 50.0% | |
| GAGAGTCCATCTTTCGCATC+TGG | + | chr3.4:45902852-45902871 | None:intergenic | 50.0% | |
| GTCAAACCTCCGGGATAGAA+AGG | + | chr3.4:45903032-45903051 | None:intergenic | 50.0% | |
| TATGCTGAAGGACCTAGGAG+TGG | - | chr3.4:45903001-45903020 | MS.gene027228:CDS | 50.0% | |
| TCTCTGTCGCTAATGGTGTG+TGG | - | chr3.4:45902064-45902083 | MS.gene027228:CDS | 50.0% | |
| TGAGCACCTGTCCAACAAAG+AGG | + | chr3.4:45903268-45903287 | None:intergenic | 50.0% | |
| ! | AGCAGCGTCTTTGAGGATGA+CGG | + | chr3.4:45902025-45902044 | None:intergenic | 50.0% |
| !! | AGGTGACGGAGTTGAGTTGA+TGG | + | chr3.4:45901990-45902009 | None:intergenic | 50.0% |
| !! | CTTTGAGGATGACGGAAACG+AGG | + | chr3.4:45902017-45902036 | None:intergenic | 50.0% |
| AAGTCTAGTCGAGGACGAGG+AGG | + | chr3.4:45903191-45903210 | None:intergenic | 55.0% | |
| ACGAGGAGGGCTTATACCCA+TGG | + | chr3.4:45903177-45903196 | None:intergenic | 55.0% | |
| AGTCTAGTCGAGGACGAGGA+GGG | + | chr3.4:45903190-45903209 | None:intergenic | 55.0% | |
| CAACGGAAGCCAACTTGGCT+TGG | + | chr3.4:45902146-45902165 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | - | chr3.4:45903278-45903297 | MS.gene027228:CDS | 55.0% | |
| GAAGCTGCTGCTGCAACCTA+TGG | - | chr3.4:45903140-45903159 | MS.gene027228:CDS | 55.0% | |
| GTTAAGCTGTTGACCACCCC+TGG | + | chr3.4:45902594-45902613 | None:intergenic | 55.0% | |
| CCATTAGCGACAGAGAGGCG+AGG | + | chr3.4:45902060-45902079 | None:intergenic | 60.0% | |
| CCTCGCCTCTCTGTCGCTAA+TGG | - | chr3.4:45902057-45902076 | MS.gene027228:CDS | 60.0% | |
| CTCAAAGACGCTGCTCCCTC+CGG | - | chr3.4:45902030-45902049 | MS.gene027228:CDS | 60.0% | |
| CTGTGTGGTACCCTCAGAGC+CGG | + | chr3.4:45901932-45901951 | None:intergenic | 60.0% | |
| GGAAACGAGGCGAGAGGTGA+CGG | + | chr3.4:45902004-45902023 | None:intergenic | 60.0% | |
| !! | ATCATCGCTGCCGGCTCTGA+GGG | - | chr3.4:45901919-45901938 | MS.gene027228:CDS | 60.0% |
| !! | GATGACGGAAACGAGGCGAG+AGG | + | chr3.4:45902010-45902029 | None:intergenic | 60.0% |
| AAAGACGCTGCTCCCTCCGG+CGG | - | chr3.4:45902033-45902052 | MS.gene027228:CDS | 65.0% | |
| CGGAGGGAGCAGCGTCTTTG+AGG | + | chr3.4:45902032-45902051 | None:intergenic | 65.0% | |
| !! | CATCATCGCTGCCGGCTCTG+AGG | - | chr3.4:45901918-45901937 | MS.gene027228:CDS | 65.0% |
| AAGACGCTGCTCCCTCCGGC+GGG | - | chr3.4:45902034-45902053 | MS.gene027228:CDS | 70.0% | |
| GACAGAGAGGCGAGGCCCGC+CGG | + | chr3.4:45902052-45902071 | None:intergenic | 75.0% | |
| ! | AGAGAGGCGAGGCCCGCCGG+AGG | + | chr3.4:45902049-45902068 | None:intergenic | 80.0% |
| ! | GAGAGGCGAGGCCCGCCGGA+GGG | + | chr3.4:45902048-45902067 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 45901790 | 45903304 | 45901790 | ID=MS.gene027228 |
| chr3.4 | mRNA | 45901790 | 45903304 | 45901790 | ID=MS.gene027228.t1;Parent=MS.gene027228 |
| chr3.4 | exon | 45902915 | 45903304 | 45902915 | ID=MS.gene027228.t1.exon1;Parent=MS.gene027228.t1 |
| chr3.4 | CDS | 45902915 | 45903304 | 45902915 | ID=cds.MS.gene027228.t1;Parent=MS.gene027228.t1 |
| chr3.4 | exon | 45901790 | 45902578 | 45901790 | ID=MS.gene027228.t1.exon2;Parent=MS.gene027228.t1 |
| chr3.4 | CDS | 45901790 | 45902578 | 45901790 | ID=cds.MS.gene027228.t1;Parent=MS.gene027228.t1 |
| Gene Sequence |
| Protein sequence |
