Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027257.t1 | XP_013455531.1 | 88.5 | 392 | 41 | 3 | 1 | 392 | 1 | 388 | 1.30E-185 | 659.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027257.t1 | Q9S7T8 | 54.0 | 389 | 174 | 5 | 5 | 390 | 4 | 390 | 5.3e-111 | 402.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027257.t1 | A0A072UIQ8 | 88.5 | 392 | 41 | 3 | 1 | 392 | 1 | 388 | 9.5e-186 | 659.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006610 | MS.gene027257 | PPI |
| MS.gene027257 | MS.gene045585 | PPI |
| MS.gene031697 | MS.gene027257 | PPI |
| MS.gene027257 | MS.gene065944 | PPI |
| MS.gene065945 | MS.gene027257 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027257.t1 | MTR_4g045707 | 88.520 | 392 | 41 | 3 | 1 | 392 | 1 | 388 | 0.0 | 686 |
| MS.gene027257.t1 | MTR_3g101010 | 72.680 | 388 | 104 | 2 | 5 | 392 | 4 | 389 | 0.0 | 536 |
| MS.gene027257.t1 | MTR_3g018740 | 68.992 | 387 | 115 | 4 | 5 | 390 | 4 | 386 | 0.0 | 518 |
| MS.gene027257.t1 | MTR_3g048000 | 65.625 | 384 | 111 | 2 | 7 | 390 | 28 | 390 | 0.0 | 508 |
| MS.gene027257.t1 | MTR_8g027395 | 66.150 | 387 | 125 | 3 | 6 | 392 | 17 | 397 | 5.79e-177 | 498 |
| MS.gene027257.t1 | MTR_3g101030 | 65.937 | 411 | 113 | 10 | 4 | 392 | 24 | 429 | 3.73e-175 | 495 |
| MS.gene027257.t1 | MTR_8g461160 | 63.706 | 394 | 137 | 4 | 1 | 390 | 1 | 392 | 2.08e-169 | 479 |
| MS.gene027257.t1 | MTR_2g046030 | 64.524 | 389 | 129 | 5 | 5 | 390 | 4 | 386 | 5.59e-169 | 478 |
| MS.gene027257.t1 | MTR_2g045980 | 65.039 | 389 | 127 | 5 | 5 | 390 | 4 | 386 | 1.01e-167 | 474 |
| MS.gene027257.t1 | MTR_8g027420 | 63.307 | 387 | 125 | 4 | 6 | 392 | 6 | 375 | 2.30e-167 | 473 |
| MS.gene027257.t1 | MTR_7g050750 | 62.437 | 394 | 138 | 5 | 5 | 392 | 7 | 396 | 6.06e-167 | 473 |
| MS.gene027257.t1 | MTR_3g101180 | 61.654 | 399 | 143 | 5 | 1 | 390 | 1 | 398 | 1.52e-165 | 469 |
| MS.gene027257.t1 | MTR_4g088525 | 65.395 | 367 | 121 | 4 | 25 | 391 | 8 | 368 | 2.19e-164 | 465 |
| MS.gene027257.t1 | MTR_7g451890 | 61.125 | 391 | 146 | 4 | 6 | 392 | 14 | 402 | 6.76e-164 | 465 |
| MS.gene027257.t1 | MTR_7g047780 | 61.637 | 391 | 144 | 4 | 6 | 392 | 14 | 402 | 3.81e-162 | 461 |
| MS.gene027257.t1 | MTR_3g101130 | 59.950 | 397 | 146 | 6 | 1 | 392 | 1 | 389 | 3.95e-159 | 453 |
| MS.gene027257.t1 | MTR_4g088535 | 61.818 | 385 | 123 | 5 | 4 | 387 | 5 | 366 | 7.26e-157 | 447 |
| MS.gene027257.t1 | MTR_7g050810 | 60.358 | 391 | 149 | 4 | 6 | 392 | 16 | 404 | 4.40e-156 | 446 |
| MS.gene027257.t1 | MTR_3g101050 | 61.461 | 397 | 122 | 8 | 3 | 391 | 24 | 397 | 2.50e-150 | 431 |
| MS.gene027257.t1 | MTR_3g015620 | 60.154 | 389 | 125 | 6 | 6 | 390 | 16 | 378 | 1.15e-143 | 413 |
| MS.gene027257.t1 | MTR_3g102920 | 57.545 | 391 | 124 | 7 | 4 | 390 | 56 | 408 | 6.24e-138 | 400 |
| MS.gene027257.t1 | MTR_3g101200 | 53.571 | 392 | 153 | 6 | 1 | 390 | 26 | 390 | 1.82e-127 | 372 |
| MS.gene027257.t1 | MTR_3g100995 | 67.568 | 259 | 79 | 4 | 134 | 392 | 11 | 264 | 4.95e-118 | 343 |
| MS.gene027257.t1 | MTR_3g101190 | 50.954 | 367 | 111 | 4 | 28 | 392 | 13 | 312 | 5.03e-118 | 345 |
| MS.gene027257.t1 | MTR_3g018747 | 65.267 | 262 | 73 | 5 | 130 | 390 | 9 | 253 | 1.26e-108 | 319 |
| MS.gene027257.t1 | MTR_3g100010 | 55.732 | 314 | 89 | 4 | 31 | 311 | 42 | 338 | 4.51e-106 | 316 |
| MS.gene027257.t1 | MTR_7g050830 | 53.165 | 316 | 113 | 5 | 6 | 299 | 16 | 318 | 1.62e-102 | 306 |
| MS.gene027257.t1 | MTR_3g015760 | 60.741 | 270 | 100 | 4 | 122 | 390 | 15 | 279 | 1.31e-98 | 295 |
| MS.gene027257.t1 | MTR_3g111160 | 67.411 | 224 | 67 | 6 | 171 | 392 | 1 | 220 | 1.02e-88 | 267 |
| MS.gene027257.t1 | MTR_3g100520 | 61.883 | 223 | 70 | 3 | 171 | 392 | 1 | 209 | 1.52e-88 | 266 |
| MS.gene027257.t1 | MTR_2g046410 | 67.633 | 207 | 61 | 4 | 187 | 390 | 144 | 347 | 1.28e-87 | 269 |
| MS.gene027257.t1 | MTR_3g114310 | 41.013 | 395 | 212 | 9 | 11 | 392 | 24 | 410 | 8.30e-87 | 270 |
| MS.gene027257.t1 | MTR_3g099970 | 65.657 | 198 | 49 | 4 | 130 | 327 | 20 | 198 | 6.72e-82 | 249 |
| MS.gene027257.t1 | MTR_3g101110 | 52.697 | 241 | 82 | 5 | 130 | 366 | 68 | 280 | 1.63e-71 | 226 |
| MS.gene027257.t1 | MTR_3g111150 | 58.757 | 177 | 49 | 1 | 8 | 184 | 1 | 153 | 9.68e-64 | 201 |
| MS.gene027257.t1 | MTR_3g101150 | 64.557 | 158 | 51 | 3 | 237 | 390 | 1 | 157 | 4.29e-60 | 192 |
| MS.gene027257.t1 | MTR_3g101020 | 57.143 | 189 | 52 | 4 | 204 | 392 | 1 | 160 | 4.02e-59 | 189 |
| MS.gene027257.t1 | MTR_3g102870 | 62.581 | 155 | 56 | 2 | 195 | 349 | 3 | 155 | 2.10e-57 | 186 |
| MS.gene027257.t1 | MTR_3g100990 | 64.122 | 131 | 47 | 0 | 4 | 134 | 3 | 133 | 5.82e-48 | 170 |
| MS.gene027257.t1 | MTR_4g093550 | 65.476 | 84 | 29 | 0 | 6 | 89 | 6 | 89 | 8.28e-30 | 110 |
| MS.gene027257.t1 | MTR_3g101140 | 64.286 | 70 | 22 | 2 | 325 | 392 | 48 | 116 | 2.34e-20 | 86.3 |
| MS.gene027257.t1 | MTR_6g046890 | 57.143 | 84 | 33 | 1 | 14 | 97 | 7 | 87 | 1.84e-19 | 84.3 |
| MS.gene027257.t1 | MTR_3g101080 | 92.593 | 27 | 2 | 0 | 356 | 382 | 13 | 39 | 7.24e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027257.t1 | AT1G47710 | 53.985 | 389 | 174 | 5 | 5 | 390 | 4 | 390 | 1.23e-143 | 414 |
| MS.gene027257.t1 | AT1G47710 | 53.985 | 389 | 174 | 5 | 5 | 390 | 31 | 417 | 4.98e-143 | 413 |
| MS.gene027257.t1 | AT3G45220 | 46.231 | 398 | 188 | 7 | 6 | 389 | 5 | 390 | 2.39e-120 | 354 |
| MS.gene027257.t1 | AT2G25240 | 48.338 | 391 | 182 | 10 | 6 | 389 | 5 | 382 | 2.35e-119 | 352 |
| MS.gene027257.t1 | AT2G26390 | 45.750 | 400 | 183 | 10 | 6 | 389 | 5 | 386 | 5.75e-111 | 330 |
| MS.gene027257.t1 | AT1G62170 | 43.622 | 392 | 191 | 6 | 6 | 391 | 66 | 433 | 9.73e-103 | 311 |
| MS.gene027257.t1 | AT1G64030 | 43.590 | 390 | 193 | 7 | 5 | 389 | 4 | 371 | 2.76e-99 | 300 |
| MS.gene027257.t1 | AT2G14540 | 44.473 | 389 | 189 | 7 | 5 | 389 | 32 | 397 | 5.70e-99 | 300 |
| MS.gene027257.t1 | AT1G62170 | 40.235 | 425 | 191 | 7 | 6 | 391 | 66 | 466 | 1.36e-95 | 293 |
| MS.gene027257.t1 | AT2G35580 | 42.199 | 391 | 183 | 9 | 5 | 389 | 18 | 371 | 1.62e-92 | 283 |
| MS.gene027257.t1 | AT1G64010 | 49.772 | 219 | 86 | 5 | 171 | 389 | 1 | 195 | 1.08e-64 | 205 |
| MS.gene027257.t1 | AT1G64010 | 49.020 | 204 | 80 | 5 | 186 | 389 | 2 | 181 | 5.22e-57 | 185 |
| MS.gene027257.t1 | AT1G62160 | 39.901 | 203 | 94 | 5 | 193 | 391 | 42 | 220 | 1.16e-33 | 125 |
| MS.gene027257.t1 | AT1G63280 | 53.043 | 115 | 51 | 2 | 130 | 243 | 8 | 120 | 4.45e-32 | 117 |
| MS.gene027257.t1 | AT1G51330 | 50.893 | 112 | 51 | 3 | 129 | 237 | 30 | 140 | 2.51e-26 | 104 |
| MS.gene027257.t1 | AT1G64020 | 38.136 | 118 | 66 | 4 | 7 | 120 | 6 | 120 | 1.25e-17 | 79.0 |
Find 98 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGGTTCAATATCTCATT+TGG | 0.243686 | 3.4:-45065600 | MS.gene027257:CDS |
| GTTGAATAACTCCTTTAATA+AGG | 0.260616 | 3.4:+45065994 | None:intergenic |
| CAGTGCCTTCTTCGTTTACT+TGG | 0.271310 | 3.4:+45065445 | None:intergenic |
| GAGACAAATGGCCTTATTAA+AGG | 0.273581 | 3.4:-45066005 | MS.gene027257:CDS |
| TTACTTGGATAAAAGACTTT+TGG | 0.279450 | 3.4:+45065460 | None:intergenic |
| AATCAGAAGAAACGATCTTT+TGG | 0.302672 | 3.4:+45066410 | None:intergenic |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.322054 | 3.4:-45065285 | MS.gene027257:CDS |
| ACACATTACATACCTTGTTC+TGG | 0.335608 | 3.4:+45066359 | None:intergenic |
| CCTCACCTCTCTGTCGCTAA+TGG | 0.345301 | 3.4:-45066472 | MS.gene027257:CDS |
| GGAAAGAAGTTTGAAGAGAA+AGG | 0.354278 | 3.4:+45066431 | None:intergenic |
| TCCCGGAGGTTTGACTAAAA+TGG | 0.356212 | 3.4:-45065526 | MS.gene027257:CDS |
| TAATAATGGCCTAGTTTATA+AGG | 0.358860 | 3.4:+45065768 | None:intergenic |
| AGAATTGACGGTCATAATAA+TGG | 0.381177 | 3.4:+45065754 | None:intergenic |
| ACCGGCACAATCCTCTTTGT+TGG | 0.389726 | 3.4:-45065309 | MS.gene027257:CDS |
| AACCCATGAATTGACTTCAT+TGG | 0.393781 | 3.4:+45066034 | None:intergenic |
| CACAATCCTCTTTGTTGGAC+AGG | 0.397313 | 3.4:-45065304 | MS.gene027257:CDS |
| GAAAGAAGTTTGAAGAGAAA+GGG | 0.401934 | 3.4:+45066432 | None:intergenic |
| CAAGGTTCAATATCTCATTT+GGG | 0.405007 | 3.4:-45065599 | MS.gene027257:CDS |
| GAGAGTCCATCTTTCGCATC+TGG | 0.410672 | 3.4:+45065714 | None:intergenic |
| CTTCAACTTCCTTATAAACT+AGG | 0.412556 | 3.4:-45065777 | MS.gene027257:CDS |
| CCCTCTACTTCAAAGGAGTA+TGG | 0.413172 | 3.4:-45065929 | MS.gene027257:CDS |
| AAAATGGTGGACTCACTTGC+CGG | 0.418030 | 3.4:-45065510 | MS.gene027257:CDS |
| GAAAGATGGACTCTCAGATT+TGG | 0.427558 | 3.4:-45065706 | MS.gene027257:CDS |
| GACAGAGAGGTGAGGCCCGC+CGG | 0.431068 | 3.4:+45066480 | None:intergenic |
| TGAAAACACAATGTTGTTGT+TGG | 0.440981 | 3.4:+45066660 | None:intergenic |
| GAAATCAACGGAAGCCAACT+TGG | 0.444733 | 3.4:+45066381 | None:intergenic |
| GATCGATTGATTTGAAACTA+AGG | 0.453659 | 3.4:+45066560 | None:intergenic |
| TGACCAATGAAGTCAATTCA+TGG | 0.453988 | 3.4:-45066037 | MS.gene027257:CDS |
| ATTCTTCCAGATGCGAAAGA+TGG | 0.456862 | 3.4:-45065720 | MS.gene027257:CDS |
| CGGAGGGAGCAGCGTCTTTG+AGG | 0.458037 | 3.4:+45066500 | None:intergenic |
| TCTATTTCACAAGTTAAAGT+AGG | 0.458733 | 3.4:-45065636 | MS.gene027257:CDS |
| CAACGGAAGCCAACTTGGCT+TGG | 0.461840 | 3.4:+45066386 | None:intergenic |
| CATCATCGCTGCCGGCTCTG+AGG | 0.467291 | 3.4:-45066611 | MS.gene027257:CDS |
| CTCTGTCGCTAATGGTGTGT+GGG | 0.474386 | 3.4:-45066464 | MS.gene027257:CDS |
| AGAAACAAACAGGTCTTGAC+CGG | 0.477188 | 3.4:+45065491 | None:intergenic |
| TTGTTTAGAGAAGAGATGTT+GGG | 0.479897 | 3.4:+45066684 | None:intergenic |
| TTTATCCAAGTAAACGAAGA+AGG | 0.480744 | 3.4:-45065450 | MS.gene027257:CDS |
| TCTCTTATTAGAAATAAGAA+TGG | 0.482752 | 3.4:+45065339 | None:intergenic |
| TAAAGTACATAGAGAATTGA+CGG | 0.488589 | 3.4:+45065742 | None:intergenic |
| ATCATCGCTGCCGGCTCTGA+GGG | 0.489637 | 3.4:-45066610 | MS.gene027257:CDS |
| GTCAAACCTCCGGGATAGAA+AGG | 0.493031 | 3.4:+45065534 | None:intergenic |
| CTCAAAGACGCTGCTCCCTC+CGG | 0.493872 | 3.4:-45066499 | MS.gene027257:CDS |
| TCTCAATGGCAGCTCAATCA+AGG | 0.494273 | 3.4:-45065859 | MS.gene027257:CDS |
| GTTAAGCTGTTGACCACCCC+TGG | 0.494814 | 3.4:+45065972 | None:intergenic |
| CTCTTATTAGAAATAAGAAT+GGG | 0.494945 | 3.4:+45065340 | None:intergenic |
| ATTGTTTAGAGAAGAGATGT+TGG | 0.505542 | 3.4:+45066683 | None:intergenic |
| CTGTGTGGTACCCTCAGAGC+CGG | 0.522370 | 3.4:+45066600 | None:intergenic |
| AAGACGCTGCTCCCTCCGGC+GGG | 0.522698 | 3.4:-45066495 | MS.gene027257:CDS |
| CTAATAAGAGAAGATTTGAC+CGG | 0.523302 | 3.4:-45065327 | MS.gene027257:CDS |
| TTGAAGTACTTTAAAACCAT+CGG | 0.525939 | 3.4:+45065794 | None:intergenic |
| GAAGCTGCTGCTGCTACCTA+TGG | 0.527503 | 3.4:-45065423 | MS.gene027257:CDS |
| CTATGGTACTTTGACAGCCA+TGG | 0.529449 | 3.4:-45065406 | MS.gene027257:CDS |
| AAAGACGCTGCTCCCTCCGG+CGG | 0.530193 | 3.4:-45066496 | MS.gene027257:CDS |
| CAATATATTCGTGCTTCCGA+TGG | 0.530199 | 3.4:-45065810 | MS.gene027257:CDS |
| TTCTGATTTCCAAGCCAAGT+TGG | 0.531191 | 3.4:-45066395 | MS.gene027257:CDS |
| ATTCTCAGCATCATCGCTGC+CGG | 0.533521 | 3.4:-45066619 | MS.gene027257:CDS |
| TCCTTCATGCTCGTCATGAA+AGG | 0.539213 | 3.4:+45065834 | None:intergenic |
| GAGAGGTGAGGCCCGCCGGA+GGG | 0.541500 | 3.4:+45066484 | None:intergenic |
| AGAAAGGTAAAACCACTCCT+AGG | 0.543661 | 3.4:+45065550 | None:intergenic |
| AGAAACGATGCGAGAGGTGA+CGG | 0.548056 | 3.4:+45066528 | None:intergenic |
| GCTAATGCCCTCTACTTCAA+AGG | 0.549267 | 3.4:-45065936 | MS.gene027257:CDS |
| TTCCGTTGATTTCCAGAACA+AGG | 0.549589 | 3.4:-45066371 | MS.gene027257:intron |
| ACCATACTCCTTTGAAGTAG+AGG | 0.553367 | 3.4:+45065928 | None:intergenic |
| AGAGAGGTGAGGCCCGCCGG+AGG | 0.560706 | 3.4:+45066483 | None:intergenic |
| AGGTGCTCAATCCTCTTGCT+GGG | 0.561778 | 3.4:-45065284 | MS.gene027257:CDS |
| GACCAATGAAGTCAATTCAT+GGG | 0.562924 | 3.4:-45066036 | MS.gene027257:CDS |
| CCAAATGAGATATTGAACCT+TGG | 0.563895 | 3.4:+45065600 | None:intergenic |
| ATTAAAGGAGTTATTCAACC+AGG | 0.564407 | 3.4:-45065990 | MS.gene027257:CDS |
| TGAAACTTCTGATATGCTGA+AGG | 0.568879 | 3.4:-45065574 | MS.gene027257:CDS |
| CATGGCTGTCAAAGTACCAT+AGG | 0.573062 | 3.4:+45065407 | None:intergenic |
| TCTCTGTCGCTAATGGTGTG+TGG | 0.581743 | 3.4:-45066465 | MS.gene027257:CDS |
| CCATACTCCTTTGAAGTAGA+GGG | 0.583430 | 3.4:+45065929 | None:intergenic |
| TCCTTTCATGACGAGCATGA+AGG | 0.586692 | 3.4:-45065835 | MS.gene027257:CDS |
| AGTCTAGTCGAGGACGAGGA+CGG | 0.587932 | 3.4:+45065376 | None:intergenic |
| TATGGTACTTTGACAGCCAT+GGG | 0.592161 | 3.4:-45065405 | MS.gene027257:CDS |
| TCTGATATGCTGAAGGACCT+AGG | 0.594866 | 3.4:-45065567 | MS.gene027257:CDS |
| TTAAAGGAGTTATTCAACCA+GGG | 0.595669 | 3.4:-45065989 | MS.gene027257:CDS |
| TAGTATGATTACCCAGCAAG+AGG | 0.598459 | 3.4:+45065273 | None:intergenic |
| CGGAGGTTTGACTAAAATGG+TGG | 0.598611 | 3.4:-45065523 | MS.gene027257:CDS |
| TCCAACAAAGAGGATTGTGC+CGG | 0.604900 | 3.4:+45065308 | None:intergenic |
| AGGAGTTATTCAACCAGGGG+TGG | 0.611289 | 3.4:-45065985 | MS.gene027257:CDS |
| TAAAGGAGTTATTCAACCAG+GGG | 0.617142 | 3.4:-45065988 | MS.gene027257:CDS |
| ACGAGGACGGCTTATACCCA+TGG | 0.623405 | 3.4:+45065389 | None:intergenic |
| TACCTTGTTCTGGAAATCAA+CGG | 0.624465 | 3.4:+45066369 | None:intergenic |
| ACAAAGTCTAGTCGAGGACG+AGG | 0.625644 | 3.4:+45065372 | None:intergenic |
| TGATTGAGCTGCCATTGAGA+AGG | 0.630337 | 3.4:+45065862 | None:intergenic |
| TGAGCACCTGTCCAACAAAG+AGG | 0.641694 | 3.4:+45065298 | None:intergenic |
| AGAATAACTTGAATCGAGAG+TGG | 0.653506 | 3.4:+45066637 | None:intergenic |
| GGTTGATCATTGATTCACGA+CGG | 0.660933 | 3.4:+45066731 | None:intergenic |
| TAGGTGACTTCAGAATTCCA+AGG | 0.662092 | 3.4:-45065617 | MS.gene027257:CDS |
| TTATTAGAAATAAGAATGGG+TGG | 0.662615 | 3.4:+45065343 | None:intergenic |
| GTGAAATAGAATGAAGCTTG+TGG | 0.680859 | 3.4:+45065649 | None:intergenic |
| TCAGCTACAAAGTCTAGTCG+AGG | 0.689387 | 3.4:+45065366 | None:intergenic |
| GATGACAGAAACGATGCGAG+AGG | 0.692315 | 3.4:+45066522 | None:intergenic |
| TATGCTGAAGGACCTAGGAG+TGG | 0.695951 | 3.4:-45065562 | MS.gene027257:CDS |
| ATCGAGAAGTTGTTGCTGTG+TGG | 0.709742 | 3.4:+45066585 | None:intergenic |
| ACACACCATTAGCGACAGAG+AGG | 0.716286 | 3.4:+45066467 | None:intergenic |
| CCATTAGCGACAGAGAGGTG+AGG | 0.728609 | 3.4:+45066472 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACAAGAAAATATAAGATT+CGG | + | chr3.4:45065812-45065831 | None:intergenic | 15.0% |
| !! | ATTTATGTTGCTAAATTAGT+TGG | + | chr3.4:45065885-45065904 | None:intergenic | 20.0% |
| !! | CTCTTATTAGAAATAAGAAT+GGG | + | chr3.4:45066683-45066702 | None:intergenic | 20.0% |
| !! | GAAATTTGATATGAATTGTT+AGG | - | chr3.4:45065951-45065970 | MS.gene027257:CDS | 20.0% |
| !! | TACAAGAAAATATAAGATTC+GGG | + | chr3.4:45065811-45065830 | None:intergenic | 20.0% |
| !! | TCTCTTATTAGAAATAAGAA+TGG | + | chr3.4:45066684-45066703 | None:intergenic | 20.0% |
| ! | GTTGAATAACTCCTTTAATA+AGG | + | chr3.4:45066029-45066048 | None:intergenic | 25.0% |
| ! | TAAAGTACATAGAGAATTGA+CGG | + | chr3.4:45066281-45066300 | None:intergenic | 25.0% |
| ! | TAATAATGGCCTAGTTTATA+AGG | + | chr3.4:45066255-45066274 | None:intergenic | 25.0% |
| ! | TAGAAAACAGTAATCAAACA+TGG | + | chr3.4:45065775-45065794 | None:intergenic | 25.0% |
| ! | TCTATTTCACAAGTTAAAGT+AGG | - | chr3.4:45066384-45066403 | MS.gene027257:CDS | 25.0% |
| ! | TTATTAGAAATAAGAATGGG+TGG | + | chr3.4:45066680-45066699 | None:intergenic | 25.0% |
| ! | TTGGATGTTAAAAAACAACA+TGG | + | chr3.4:45065866-45065885 | None:intergenic | 25.0% |
| !! | AAGGTATGTAATGTGTTTTA+GGG | - | chr3.4:45065668-45065687 | MS.gene027257:CDS | 25.0% |
| !! | CATGAGTATAAGTTTAGTTT+AGG | - | chr3.4:45065746-45065765 | MS.gene027257:CDS | 25.0% |
| !! | TTACTTGGATAAAAGACTTT+TGG | + | chr3.4:45066563-45066582 | None:intergenic | 25.0% |
| !!! | GGAATATTTTAGAAACAAAC+AGG | + | chr3.4:45066542-45066561 | None:intergenic | 25.0% |
| !!! | TTGAAGTACTTTAAAACCAT+CGG | + | chr3.4:45066229-45066248 | None:intergenic | 25.0% |
| AGAATTGACGGTCATAATAA+TGG | + | chr3.4:45066269-45066288 | None:intergenic | 30.0% | |
| ATTAAAGGAGTTATTCAACC+AGG | - | chr3.4:45066030-45066049 | MS.gene027257:CDS | 30.0% | |
| ATTGTTTAGAGAAGAGATGT+TGG | + | chr3.4:45065340-45065359 | None:intergenic | 30.0% | |
| CAAGGTTCAATATCTCATTT+GGG | - | chr3.4:45066421-45066440 | MS.gene027257:CDS | 30.0% | |
| CTAATAAGAGAAGATTTGAC+CGG | - | chr3.4:45066693-45066712 | MS.gene027257:CDS | 30.0% | |
| CTTCAACTTCCTTATAAACT+AGG | - | chr3.4:45066243-45066262 | MS.gene027257:intron | 30.0% | |
| GAAAGAAGTTTGAAGAGAAA+GGG | + | chr3.4:45065591-45065610 | None:intergenic | 30.0% | |
| TGAAAACACAATGTTGTTGT+TGG | + | chr3.4:45065363-45065382 | None:intergenic | 30.0% | |
| TTAAAGGAGTTATTCAACCA+GGG | - | chr3.4:45066031-45066050 | MS.gene027257:CDS | 30.0% | |
| TTGTTTAGAGAAGAGATGTT+GGG | + | chr3.4:45065339-45065358 | None:intergenic | 30.0% | |
| TTTATCCAAGTAAACGAAGA+AGG | - | chr3.4:45066570-45066589 | MS.gene027257:CDS | 30.0% | |
| ! | AATCAGAAGAAACGATCTTT+TGG | + | chr3.4:45065613-45065632 | None:intergenic | 30.0% |
| ! | CAAGGTATGTAATGTGTTTT+AGG | - | chr3.4:45065667-45065686 | MS.gene027257:CDS | 30.0% |
| ! | TATGATTTTCACCTTCTCAA+TGG | - | chr3.4:45066147-45066166 | MS.gene027257:intron | 30.0% |
| !! | GATCGATTGATTTGAAACTA+AGG | + | chr3.4:45065463-45065482 | None:intergenic | 30.0% |
| ACACATTACATACCTTGTTC+TGG | + | chr3.4:45065664-45065683 | None:intergenic | 35.0% | |
| AGAATAACTTGAATCGAGAG+TGG | + | chr3.4:45065386-45065405 | None:intergenic | 35.0% | |
| CCAAATGAGATATTGAACCT+TGG | + | chr3.4:45066423-45066442 | None:intergenic | 35.0% | |
| CCAAGGTTCAATATCTCATT+TGG | - | chr3.4:45066420-45066439 | MS.gene027257:CDS | 35.0% | |
| GACCAATGAAGTCAATTCAT+GGG | - | chr3.4:45065984-45066003 | MS.gene027257:CDS | 35.0% | |
| GGAAAGAAGTTTGAAGAGAA+AGG | + | chr3.4:45065592-45065611 | None:intergenic | 35.0% | |
| GTGAAATAGAATGAAGCTTG+TGG | + | chr3.4:45066374-45066393 | None:intergenic | 35.0% | |
| TAAAGGAGTTATTCAACCAG+GGG | - | chr3.4:45066032-45066051 | MS.gene027257:CDS | 35.0% | |
| TGAAACTTCTGATATGCTGA+AGG | - | chr3.4:45066446-45066465 | MS.gene027257:CDS | 35.0% | |
| TGACCAATGAAGTCAATTCA+TGG | - | chr3.4:45065983-45066002 | MS.gene027257:CDS | 35.0% | |
| ! | AACCCATGAATTGACTTCAT+TGG | + | chr3.4:45065989-45066008 | None:intergenic | 35.0% |
| ! | TGGGTTGAAAAAGAGACAAA+TGG | - | chr3.4:45066003-45066022 | MS.gene027257:CDS | 35.0% |
| !! | GAGACAAATGGCCTTATTAA+AGG | - | chr3.4:45066015-45066034 | MS.gene027257:CDS | 35.0% |
| !! | TACCTTGTTCTGGAAATCAA+CGG | + | chr3.4:45065654-45065673 | None:intergenic | 35.0% |
| !! | TTTTGAGTGAAACGTTGGTT+TGG | + | chr3.4:45065313-45065332 | None:intergenic | 35.0% |
| AGAAACAAACAGGTCTTGAC+CGG | + | chr3.4:45066532-45066551 | None:intergenic | 40.0% | |
| AGAAAGGTAAAACCACTCCT+AGG | + | chr3.4:45066473-45066492 | None:intergenic | 40.0% | |
| ATTCTTCCAGATGCGAAAGA+TGG | - | chr3.4:45066300-45066319 | MS.gene027257:intron | 40.0% | |
| CAATATATTCGTGCTTCCGA+TGG | - | chr3.4:45066210-45066229 | MS.gene027257:intron | 40.0% | |
| TAGGTGACTTCAGAATTCCA+AGG | - | chr3.4:45066403-45066422 | MS.gene027257:CDS | 40.0% | |
| TATGGTACTTTGACAGCCAT+GGG | - | chr3.4:45066615-45066634 | MS.gene027257:CDS | 40.0% | |
| TTCTGATTTCCAAGCCAAGT+TGG | - | chr3.4:45065625-45065644 | MS.gene027257:CDS | 40.0% | |
| ! | GAAAGATGGACTCTCAGATT+TGG | - | chr3.4:45066314-45066333 | MS.gene027257:intron | 40.0% |
| !! | ACCATACTCCTTTGAAGTAG+AGG | + | chr3.4:45066095-45066114 | None:intergenic | 40.0% |
| !! | ACCATTTTAGTCAAACCTCC+GGG | + | chr3.4:45066498-45066517 | None:intergenic | 40.0% |
| !! | CACCATTTTAGTCAAACCTC+CGG | + | chr3.4:45066499-45066518 | None:intergenic | 40.0% |
| !! | CCATACTCCTTTGAAGTAGA+GGG | + | chr3.4:45066094-45066113 | None:intergenic | 40.0% |
| !! | GGTTGATCATTGATTCACGA+CGG | + | chr3.4:45065292-45065311 | None:intergenic | 40.0% |
| !! | GTGGTTTTACCTTTCTATCC+CGG | - | chr3.4:45066477-45066496 | MS.gene027257:CDS | 40.0% |
| !! | TTCCGTTGATTTCCAGAACA+AGG | - | chr3.4:45065649-45065668 | MS.gene027257:CDS | 40.0% |
| ATCGAGAAGTTGTTGCTGTG+TGG | + | chr3.4:45065438-45065457 | None:intergenic | 45.0% | |
| CACAATCCTCTTTGTTGGAC+AGG | - | chr3.4:45066716-45066735 | MS.gene027257:CDS | 45.0% | |
| CAGTGCCTTCTTCGTTTACT+TGG | + | chr3.4:45066578-45066597 | None:intergenic | 45.0% | |
| CCCTCTACTTCAAAGGAGTA+TGG | - | chr3.4:45066091-45066110 | MS.gene027257:intron | 45.0% | |
| CGGAGGTTTGACTAAAATGG+TGG | - | chr3.4:45066497-45066516 | MS.gene027257:CDS | 45.0% | |
| CTATGGTACTTTGACAGCCA+TGG | - | chr3.4:45066614-45066633 | MS.gene027257:CDS | 45.0% | |
| GAAATCAACGGAAGCCAACT+TGG | + | chr3.4:45065642-45065661 | None:intergenic | 45.0% | |
| GCTAATGCCCTCTACTTCAA+AGG | - | chr3.4:45066084-45066103 | MS.gene027257:intron | 45.0% | |
| TCAGCTACAAAGTCTAGTCG+AGG | + | chr3.4:45066657-45066676 | None:intergenic | 45.0% | |
| TCCAACAAAGAGGATTGTGC+CGG | + | chr3.4:45066715-45066734 | None:intergenic | 45.0% | |
| TCCCGGAGGTTTGACTAAAA+TGG | - | chr3.4:45066494-45066513 | MS.gene027257:CDS | 45.0% | |
| TCCTTCATGCTCGTCATGAA+AGG | + | chr3.4:45066189-45066208 | None:intergenic | 45.0% | |
| TCCTTTCATGACGAGCATGA+AGG | - | chr3.4:45066185-45066204 | MS.gene027257:intron | 45.0% | |
| TCTCAATGGCAGCTCAATCA+AGG | - | chr3.4:45066161-45066180 | MS.gene027257:intron | 45.0% | |
| TCTGATATGCTGAAGGACCT+AGG | - | chr3.4:45066453-45066472 | MS.gene027257:CDS | 45.0% | |
| TGATTGAGCTGCCATTGAGA+AGG | + | chr3.4:45066161-45066180 | None:intergenic | 45.0% | |
| ! | CATGGCTGTCAAAGTACCAT+AGG | + | chr3.4:45066616-45066635 | None:intergenic | 45.0% |
| ! | GTTTTACCTTTCTATCCCGG+AGG | - | chr3.4:45066480-45066499 | MS.gene027257:CDS | 45.0% |
| !! | AAAATGGTGGACTCACTTGC+CGG | - | chr3.4:45066510-45066529 | MS.gene027257:CDS | 45.0% |
| !! | GGCGATTTTGAGTGAAACGT+TGG | + | chr3.4:45065318-45065337 | None:intergenic | 45.0% |
| ACAAAGTCTAGTCGAGGACG+AGG | + | chr3.4:45066651-45066670 | None:intergenic | 50.0% | |
| ACACACCATTAGCGACAGAG+AGG | + | chr3.4:45065556-45065575 | None:intergenic | 50.0% | |
| ACCGGCACAATCCTCTTTGT+TGG | - | chr3.4:45066711-45066730 | MS.gene027257:CDS | 50.0% | |
| AGAAACGATGCGAGAGGTGA+CGG | + | chr3.4:45065495-45065514 | None:intergenic | 50.0% | |
| AGGAGTTATTCAACCAGGGG+TGG | - | chr3.4:45066035-45066054 | MS.gene027257:CDS | 50.0% | |
| AGGTGCTCAATCCTCTTGCT+GGG | - | chr3.4:45066736-45066755 | MS.gene027257:CDS | 50.0% | |
| ATTCTCAGCATCATCGCTGC+CGG | - | chr3.4:45065401-45065420 | MS.gene027257:CDS | 50.0% | |
| CTCTGTCGCTAATGGTGTGT+GGG | - | chr3.4:45065556-45065575 | MS.gene027257:CDS | 50.0% | |
| GAGAGTCCATCTTTCGCATC+TGG | + | chr3.4:45066309-45066328 | None:intergenic | 50.0% | |
| GATGACAGAAACGATGCGAG+AGG | + | chr3.4:45065501-45065520 | None:intergenic | 50.0% | |
| GTCAAACCTCCGGGATAGAA+AGG | + | chr3.4:45066489-45066508 | None:intergenic | 50.0% | |
| TATGCTGAAGGACCTAGGAG+TGG | - | chr3.4:45066458-45066477 | MS.gene027257:CDS | 50.0% | |
| TCTCTGTCGCTAATGGTGTG+TGG | - | chr3.4:45065555-45065574 | MS.gene027257:CDS | 50.0% | |
| TGAGCACCTGTCCAACAAAG+AGG | + | chr3.4:45066725-45066744 | None:intergenic | 50.0% | |
| ACGAGGACGGCTTATACCCA+TGG | + | chr3.4:45066634-45066653 | None:intergenic | 55.0% | |
| AGTCTAGTCGAGGACGAGGA+CGG | + | chr3.4:45066647-45066666 | None:intergenic | 55.0% | |
| CAACGGAAGCCAACTTGGCT+TGG | + | chr3.4:45065637-45065656 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | - | chr3.4:45066735-45066754 | MS.gene027257:CDS | 55.0% | |
| CCATTAGCGACAGAGAGGTG+AGG | + | chr3.4:45065551-45065570 | None:intergenic | 55.0% | |
| CCTCACCTCTCTGTCGCTAA+TGG | - | chr3.4:45065548-45065567 | MS.gene027257:CDS | 55.0% | |
| GAAGCTGCTGCTGCTACCTA+TGG | - | chr3.4:45066597-45066616 | MS.gene027257:CDS | 55.0% | |
| GTTAAGCTGTTGACCACCCC+TGG | + | chr3.4:45066051-45066070 | None:intergenic | 55.0% | |
| CTCAAAGACGCTGCTCCCTC+CGG | - | chr3.4:45065521-45065540 | MS.gene027257:CDS | 60.0% | |
| CTGTGTGGTACCCTCAGAGC+CGG | + | chr3.4:45065423-45065442 | None:intergenic | 60.0% | |
| !! | ATCATCGCTGCCGGCTCTGA+GGG | - | chr3.4:45065410-45065429 | MS.gene027257:CDS | 60.0% |
| AAAGACGCTGCTCCCTCCGG+CGG | - | chr3.4:45065524-45065543 | MS.gene027257:CDS | 65.0% | |
| CGGAGGGAGCAGCGTCTTTG+AGG | + | chr3.4:45065523-45065542 | None:intergenic | 65.0% | |
| !! | CATCATCGCTGCCGGCTCTG+AGG | - | chr3.4:45065409-45065428 | MS.gene027257:CDS | 65.0% |
| AAGACGCTGCTCCCTCCGGC+GGG | - | chr3.4:45065525-45065544 | MS.gene027257:CDS | 70.0% | |
| GACAGAGAGGTGAGGCCCGC+CGG | + | chr3.4:45065543-45065562 | None:intergenic | 70.0% | |
| AGAGAGGTGAGGCCCGCCGG+AGG | + | chr3.4:45065540-45065559 | None:intergenic | 75.0% | |
| GAGAGGTGAGGCCCGCCGGA+GGG | + | chr3.4:45065539-45065558 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 45065281 | 45066761 | 45065281 | ID=MS.gene027257 |
| chr3.4 | mRNA | 45065281 | 45066761 | 45065281 | ID=MS.gene027257.t1;Parent=MS.gene027257 |
| chr3.4 | exon | 45066372 | 45066761 | 45066372 | ID=MS.gene027257.t1.exon1;Parent=MS.gene027257.t1 |
| chr3.4 | CDS | 45066372 | 45066761 | 45066372 | ID=cds.MS.gene027257.t1;Parent=MS.gene027257.t1 |
| chr3.4 | exon | 45065281 | 45066069 | 45065281 | ID=MS.gene027257.t1.exon2;Parent=MS.gene027257.t1 |
| chr3.4 | CDS | 45065281 | 45066069 | 45065281 | ID=cds.MS.gene027257.t1;Parent=MS.gene027257.t1 |
| Gene Sequence |
| Protein sequence |
