AlfalfaGEDB | MS.gene028667

M. sativa cultivar XinJiangDaYe / MS.gene028667

KEGG: -
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028667.t1	XP_013465354.1	97.7	131	3	0	1	131	1	131	1.10E-67	265.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028667.t1	P0DKL1	37.5	96	52	3	6	101	4	91	1.6e-11	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028667.t1	G8A150	97.7	131	3	0	1	131	1	131	7.8e-68	265.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene028667	MS.gene20078	PPI
MS.gene028667	MS.gene22418	PPI
MS.gene007417	MS.gene028667	PPI
MS.gene028667	MS.gene007530	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028667.t1	MTR_2g095430	97.710	131	3	0	1	131	1	131	2.11e-94	268
MS.gene028667.t1	MTR_4g057800	56.190	105	42	1	16	116	5	109	4.05e-37	123
MS.gene028667.t1	MTR_4g057810	53.333	105	43	2	16	116	5	107	5.02e-35	117
MS.gene028667.t1	MTR_1g063340	41.837	98	52	2	18	112	2	97	2.51e-21	82.4
MS.gene028667.t1	MTR_1g063330	40.000	95	52	2	18	109	2	94	2.70e-19	77.0
MS.gene028667.t1	MTR_1g063310	43.158	95	49	2	21	115	5	94	3.65e-19	76.6
MS.gene028667.t1	MTR_1g063300	47.297	74	37	1	37	110	31	102	1.06e-18	75.9
MS.gene028667.t1	MTR_1g063290	48.571	70	34	1	41	110	35	102	1.10e-18	75.9
MS.gene028667.t1	MTR_7g101890	50.000	76	32	2	37	112	48	117	6.30e-18	74.3
MS.gene028667.t1	MTR_1g063260	40.506	79	45	1	34	112	30	106	1.65e-17	72.8
MS.gene028667.t1	MTR_1g063280	35.238	105	53	3	21	112	5	107	2.79e-16	69.7
MS.gene028667.t1	MTR_7g101920	43.678	87	44	3	40	124	30	113	7.61e-16	68.6
MS.gene028667.t1	MTR_4g072980	51.471	68	29	2	35	101	17	81	4.39e-15	66.2
MS.gene028667.t1	MTR_8g076040	39.175	97	52	3	5	100	2	92	9.05e-15	65.5
MS.gene028667.t1	MTR_3g117610	49.180	61	27	1	41	101	41	97	1.04e-14	65.5
MS.gene028667.t1	MTR_4g072190	48.333	60	27	1	42	101	33	88	6.98e-14	63.2
MS.gene028667.t1	MTR_5g021820	45.000	60	29	1	41	100	36	91	1.32e-13	62.8
MS.gene028667.t1	MTR_4g072820	43.662	71	35	2	32	101	7	73	1.65e-13	62.0
MS.gene028667.t1	MTR_0388s0020	45.614	57	31	0	52	108	81	137	4.26e-13	62.4
MS.gene028667.t1	MTR_4g072910	42.353	85	41	3	17	100	10	87	4.36e-13	61.2
MS.gene028667.t1	MTR_3g117640	50.847	59	25	2	44	101	33	88	6.32e-13	60.8
MS.gene028667.t1	MTR_7g118010	52.174	46	22	0	60	105	36	81	6.83e-13	60.8
MS.gene028667.t1	MTR_8g461210	38.542	96	52	2	18	110	5	96	1.34e-12	60.5
MS.gene028667.t1	MTR_4g072310	40.964	83	42	3	27	108	10	86	1.38e-12	59.7
MS.gene028667.t1	MTR_4g072300	42.424	66	33	2	44	108	25	86	1.93e-12	59.3
MS.gene028667.t1	MTR_1g024085	38.824	85	45	2	19	100	8	88	4.42e-12	59.7
MS.gene028667.t1	MTR_3g092220	45.161	62	32	2	44	105	32	91	6.16e-12	58.5
MS.gene028667.t1	MTR_3g113310	45.614	57	27	1	44	100	41	93	6.64e-12	58.2
MS.gene028667.t1	MTR_4g072880	45.588	68	30	3	34	100	17	78	8.11e-12	57.8
MS.gene028667.t1	MTR_4g072240	36.364	88	49	3	22	108	5	86	8.16e-12	57.8
MS.gene028667.t1	MTR_4g025830	42.188	64	36	1	45	108	79	141	8.50e-12	59.3
MS.gene028667.t1	MTR_4g072370	41.096	73	38	2	30	101	8	76	9.47e-12	57.4
MS.gene028667.t1	MTR_4g072220	42.424	66	33	2	44	108	25	86	1.10e-11	57.4
MS.gene028667.t1	MTR_4g072870	47.458	59	26	2	44	101	25	79	1.35e-11	57.0
MS.gene028667.t1	MTR_4g072890	44.444	63	30	2	44	105	25	83	1.59e-11	57.0
MS.gene028667.t1	MTR_4g072250	50.000	58	23	3	44	100	25	77	1.91e-11	56.6
MS.gene028667.t1	MTR_8g026730	50.000	46	23	0	60	105	40	85	1.92e-11	57.0
MS.gene028667.t1	MTR_4g072260	46.552	58	26	2	44	100	25	78	2.48e-11	56.6
MS.gene028667.t1	MTR_4g072860	47.059	68	29	3	34	100	17	78	2.97e-11	56.2
MS.gene028667.t1	MTR_4g072330	45.763	59	27	2	44	101	22	76	2.98e-11	56.2
MS.gene028667.t1	MTR_4g072270	41.333	75	37	3	27	100	10	78	3.20e-11	56.2
MS.gene028667.t1	MTR_4g072540	49.153	59	25	2	44	101	20	74	3.44e-11	55.8
MS.gene028667.t1	MTR_4g124750	35.955	89	50	2	16	101	37	121	5.49e-11	57.0
MS.gene028667.t1	MTR_4g072770	55.814	43	18	1	60	101	26	68	7.12e-11	55.1
MS.gene028667.t1	MTR_4g072480	47.458	59	26	2	44	101	24	78	7.70e-11	55.1
MS.gene028667.t1	MTR_2g043960	46.667	60	28	1	42	101	73	128	8.89e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028667.t1	AT2G28085	45.000	120	55	3	3	113	6	123	6.83e-27	97.4
MS.gene028667.t1	AT3G09870	54.667	75	32	1	36	110	36	108	6.25e-21	81.6
MS.gene028667.t1	AT1G19830	43.243	74	38	1	41	114	44	113	5.19e-15	66.6
MS.gene028667.t1	AT4G34780	43.210	81	44	2	44	123	27	106	2.16e-14	64.7
MS.gene028667.t1	AT4G34760	37.634	93	35	2	8	100	25	94	5.22e-14	63.9
MS.gene028667.t1	AT4G34810	40.964	83	47	2	33	114	37	118	1.07e-13	63.2
MS.gene028667.t1	AT3G61900	48.485	66	33	1	35	100	20	84	1.23e-13	63.5
MS.gene028667.t1	AT3G43120	42.683	82	41	2	42	121	76	153	2.27e-13	63.5
MS.gene028667.t1	AT2G21220	45.902	61	29	1	41	101	36	92	3.22e-13	61.6
MS.gene028667.t1	AT2G16580	37.634	93	36	2	8	100	25	95	3.90e-13	61.6
MS.gene028667.t1	AT4G38840	53.448	58	23	2	44	100	32	86	5.45e-13	60.8
MS.gene028667.t1	AT4G34800	51.724	58	26	2	45	101	21	77	8.73e-13	60.5
MS.gene028667.t1	AT1G75580	48.333	60	27	1	42	101	41	96	8.82e-13	60.5
MS.gene028667.t1	AT4G38825	44.000	75	32	3	27	100	11	76	1.56e-12	59.7
MS.gene028667.t1	AT5G10990	43.023	86	45	2	18	103	14	95	1.70e-12	60.8
MS.gene028667.t1	AT4G38860	46.774	62	28	2	41	101	36	93	2.68e-12	59.3
MS.gene028667.t1	AT2G46690	38.554	83	47	2	23	105	5	83	3.24e-12	59.7
MS.gene028667.t1	AT5G20810	50.000	60	26	1	42	101	76	131	3.45e-12	60.5
MS.gene028667.t1	AT5G20810	48.438	64	29	1	38	101	72	131	3.78e-12	60.8
MS.gene028667.t1	AT5G18030	45.333	75	30	4	27	100	11	75	3.83e-12	58.5
MS.gene028667.t1	AT4G38850	42.857	63	35	1	50	111	25	87	5.03e-12	58.2
MS.gene028667.t1	AT5G18020	35.870	92	50	4	25	115	8	91	7.60e-12	57.8
MS.gene028667.t1	AT4G34770	40.000	85	48	2	17	100	7	89	7.80e-12	58.2
MS.gene028667.t1	AT1G19840	38.298	94	52	2	18	110	14	102	8.51e-12	59.3
MS.gene028667.t1	AT2G18010	40.476	84	45	2	18	100	20	99	1.56e-11	57.4
MS.gene028667.t1	AT2G21210	47.368	57	27	2	45	100	28	82	1.84e-11	57.0
MS.gene028667.t1	AT5G18050	40.260	77	38	3	25	100	8	77	2.08e-11	56.6
MS.gene028667.t1	AT5G53590	44.444	63	34	1	44	105	45	107	2.31e-11	57.8
MS.gene028667.t1	AT5G18060	41.558	77	37	3	25	100	8	77	2.90e-11	56.2
MS.gene028667.t1	AT5G18080	40.260	77	38	3	25	100	8	77	3.67e-11	55.8
MS.gene028667.t1	AT2G21210	47.368	57	27	2	45	100	95	149	4.57e-11	57.4
MS.gene028667.t1	AT2G45210	35.526	76	42	2	39	108	67	141	8.08e-11	56.6

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATTAGTTAAACAACTTAA+TGG	0.258123	2.1:+8124240	None:intergenic
CTACAAAAGAAAATTGTAAT+AGG	0.272237	2.1:-8124369	MS.gene028667:CDS
TCCAACATTTGTAAGGCTAT+TGG	0.311111	2.1:-8124220	MS.gene028667:CDS
GAGAAAACAGAAGAAGAGTA+TGG	0.352500	2.1:-8124198	MS.gene028667:CDS
TAGGTTTGCAAGGAATGGTT+AGG	0.372700	2.1:+8124157	None:intergenic
TCCAATAGCCTTACAAATGT+TGG	0.391382	2.1:+8124219	None:intergenic
TAACTAATCCAACATTTGTA+AGG	0.401955	2.1:-8124227	MS.gene028667:CDS
TACAAAAGAAAATTGTAATA+GGG	0.412265	2.1:-8124368	MS.gene028667:CDS
TGTCCTTCCTTTACATCCTC+TGG	0.426410	2.1:+8124318	None:intergenic
GTGTGAGAAGCATCAACAAA+AGG	0.471114	2.1:-8124428	MS.gene028667:CDS
TTCACTAGGTTTGCAAGGAA+TGG	0.477249	2.1:+8124152	None:intergenic
CTTTACATCCTCTGGCACAT+AGG	0.520298	2.1:+8124326	None:intergenic
TGAAGTTCACTAGGTTTGCA+AGG	0.531515	2.1:+8124147	None:intergenic
AACAAAAGGAATGGTGGAGG+TGG	0.543634	2.1:-8124414	MS.gene028667:CDS
CTATGTGCCAGAGGATGTAA+AGG	0.546374	2.1:-8124325	MS.gene028667:CDS
AGGTTTGCAAGGAATGGTTA+GGG	0.558413	2.1:+8124158	None:intergenic
AACATCTTGTGAAGTTCACT+AGG	0.579992	2.1:+8124138	None:intergenic
AGAAGCATCAACAAAAGGAA+TGG	0.589968	2.1:-8124423	MS.gene028667:CDS
ACAAGATGTTGCAACAGCAA+TGG	0.597792	2.1:-8124125	MS.gene028667:CDS
TTGCTGTGATTGCTAAGGGT+AGG	0.602902	2.1:-8124293	MS.gene028667:CDS
GATGCTTCTCACACTGAACA+TGG	0.608352	2.1:+8124437	None:intergenic
AGCATCAACAAAAGGAATGG+TGG	0.610322	2.1:-8124420	MS.gene028667:CDS
TGTGATTGCTAAGGGTAGGA+AGG	0.616305	2.1:-8124289	MS.gene028667:CDS
GTGCCAGAGGATGTAAAGGA+AGG	0.617148	2.1:-8124321	MS.gene028667:CDS
ATCAACAAAAGGAATGGTGG+AGG	0.620965	2.1:-8124417	MS.gene028667:CDS
AACATCAACCTATGTGCCAG+AGG	0.654930	2.1:-8124334	MS.gene028667:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACAAAAGAAAATTGTAATA+GGG	-	chr2.1:8124141-8124160	MS.gene028667:CDS	15.0%
!!	CTACAAAAGAAAATTGTAAT+AGG	-	chr2.1:8124140-8124159	MS.gene028667:CDS	20.0%
!	TAACTAATCCAACATTTGTA+AGG	-	chr2.1:8124282-8124301	MS.gene028667:CDS	25.0%
!!!	GGATTAGTTAAACAACTTAA+TGG	+	chr2.1:8124272-8124291	None:intergenic	25.0%
	AACATCTTGTGAAGTTCACT+AGG	+	chr2.1:8124374-8124393	None:intergenic	35.0%
	GAGAAAACAGAAGAAGAGTA+TGG	-	chr2.1:8124311-8124330	MS.gene028667:CDS	35.0%
	TCCAACATTTGTAAGGCTAT+TGG	-	chr2.1:8124289-8124308	MS.gene028667:CDS	35.0%
!	TCCAATAGCCTTACAAATGT+TGG	+	chr2.1:8124293-8124312	None:intergenic	35.0%
!!	ACATTTTGCTGTGATTGCTA+AGG	-	chr2.1:8124211-8124230	MS.gene028667:CDS	35.0%
!!	AGAAGCATCAACAAAAGGAA+TGG	-	chr2.1:8124086-8124105	MS.gene028667:CDS	35.0%
!!	CATTTTGCTGTGATTGCTAA+GGG	-	chr2.1:8124212-8124231	MS.gene028667:CDS	35.0%
!!!	GAGTATGGTTTTGATCATCA+AGG	-	chr2.1:8124326-8124345	MS.gene028667:CDS	35.0%
	ACAAGATGTTGCAACAGCAA+TGG	-	chr2.1:8124384-8124403	MS.gene028667:CDS	40.0%
	ACAGCAATGGCAAAAACAAG+AGG	-	chr2.1:8124397-8124416	MS.gene028667:CDS	40.0%
	AGCATCAACAAAAGGAATGG+TGG	-	chr2.1:8124089-8124108	MS.gene028667:CDS	40.0%
	AGGTTTGCAAGGAATGGTTA+GGG	+	chr2.1:8124354-8124373	None:intergenic	40.0%
	ATCAACAAAAGGAATGGTGG+AGG	-	chr2.1:8124092-8124111	MS.gene028667:CDS	40.0%
	CAGCAATGGCAAAAACAAGA+GGG	-	chr2.1:8124398-8124417	MS.gene028667:CDS	40.0%
	TAGGTTTGCAAGGAATGGTT+AGG	+	chr2.1:8124355-8124374	None:intergenic	40.0%
	TGAAGTTCACTAGGTTTGCA+AGG	+	chr2.1:8124365-8124384	None:intergenic	40.0%
	TTCACTAGGTTTGCAAGGAA+TGG	+	chr2.1:8124360-8124379	None:intergenic	40.0%
!!	GTGTGAGAAGCATCAACAAA+AGG	-	chr2.1:8124081-8124100	MS.gene028667:CDS	40.0%
	AACATCAACCTATGTGCCAG+AGG	-	chr2.1:8124175-8124194	MS.gene028667:CDS	45.0%
	CAATGGCAAAAACAAGAGGG+AGG	-	chr2.1:8124401-8124420	MS.gene028667:CDS	45.0%
	CTATGTGCCAGAGGATGTAA+AGG	-	chr2.1:8124184-8124203	MS.gene028667:CDS	45.0%
	CTTTACATCCTCTGGCACAT+AGG	+	chr2.1:8124186-8124205	None:intergenic	45.0%
	GATGCTTCTCACACTGAACA+TGG	+	chr2.1:8124075-8124094	None:intergenic	45.0%
	TGTCCTTCCTTTACATCCTC+TGG	+	chr2.1:8124194-8124213	None:intergenic	45.0%
	TGTGATTGCTAAGGGTAGGA+AGG	-	chr2.1:8124220-8124239	MS.gene028667:CDS	45.0%
	TTGCTGTGATTGCTAAGGGT+AGG	-	chr2.1:8124216-8124235	MS.gene028667:CDS	45.0%
!	AACAAAAGGAATGGTGGAGG+TGG	-	chr2.1:8124095-8124114	MS.gene028667:CDS	45.0%
	GTGCCAGAGGATGTAAAGGA+AGG	-	chr2.1:8124188-8124207	MS.gene028667:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	8124068	8124463	8124068	ID=MS.gene028667
chr2.1	mRNA	8124068	8124463	8124068	ID=MS.gene028667.t1;Parent=MS.gene028667
chr2.1	exon	8124068	8124463	8124068	ID=MS.gene028667.t1.exon1;Parent=MS.gene028667.t1
chr2.1	CDS	8124068	8124463	8124068	ID=cds.MS.gene028667.t1;Parent=MS.gene028667.t1

Gene Sequence

>MS.gene028667

ATGGCCATGTTCAGTGTGAGAAGCATCAACAAAAGGAATGGTGGAGGTGGCATTGTGAAGCTCATTGAGAAGCTACAAAAGAAAATTGTAATAGGGAGAAACAAATCAACATCAACCTATGTGCCAGAGGATGTAAAGGAAGGACATTTTGCTGTGATTGCTAAGGGTAGGAAGGAAGAAGAAGCAAAGAGATTTGTGTTACCATTAAGTTGTTTAACTAATCCAACATTTGTAAGGCTATTGGAGAAAACAGAAGAAGAGTATGGTTTTGATCATCAAGGTGCCCTAACCATTCCTTGCAAACCTAGTGAACTTCACAAGATGTTGCAACAGCAATGGCAAAAACAAGAGGGAGGTGCTGAAATTAACTGCTACAACATGCAATTCCTCCAATAA

Protein sequence

>MS.gene028667.t1

MAMFSVRSINKRNGGGGIVKLIEKLQKKIVIGRNKSTSTYVPEDVKEGHFAVIAKGRKEEEAKRFVLPLSCLTNPTFVRLLEKTEEEYGFDHQGALTIPCKPSELHKMLQQQWQKQEGGAEINCYNMQFLQ