Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030163.t1 | XP_003609880.1 | 99 | 624 | 6 | 0 | 1 | 624 | 1 | 624 | 0.00E+00 | 1249.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030163.t1 | Q54XC1 | 28.8 | 528 | 303 | 9 | 118 | 603 | 86 | 582 | 8.6e-58 | 227.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030163.t1 | G7JR10 | 99.0 | 624 | 6 | 0 | 1 | 624 | 1 | 624 | 0.0e+00 | 1249.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene030163 | MS.gene42341 | PPI |
| MS.gene030163 | MS.gene064666 | PPI |
| MS.gene030163 | MS.gene014516 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030163.t1 | MTR_4g123980 | 99.038 | 624 | 6 | 0 | 1 | 624 | 1 | 624 | 0.0 | 1293 |
| MS.gene030163.t1 | MTR_4g123980 | 92.308 | 624 | 6 | 1 | 1 | 624 | 1 | 582 | 0.0 | 1186 |
| MS.gene030163.t1 | MTR_5g082530 | 31.599 | 519 | 296 | 14 | 121 | 602 | 10 | 506 | 3.75e-68 | 238 |
| MS.gene030163.t1 | MTR_3g111870 | 31.569 | 510 | 307 | 12 | 124 | 602 | 32 | 530 | 2.22e-67 | 238 |
| MS.gene030163.t1 | MTR_8g070580 | 39.826 | 344 | 150 | 8 | 753 | 1095 | 27 | 314 | 4.78e-64 | 226 |
| MS.gene030163.t1 | MTR_1g022260 | 31.648 | 534 | 314 | 12 | 107 | 602 | 93 | 613 | 1.90e-62 | 224 |
| MS.gene030163.t1 | MTR_4g129920 | 27.767 | 497 | 314 | 13 | 103 | 588 | 54 | 516 | 2.18e-43 | 167 |
| MS.gene030163.t1 | MTR_4g028910 | 37.549 | 253 | 87 | 5 | 843 | 1095 | 163 | 344 | 1.48e-32 | 132 |
| MS.gene030163.t1 | MTR_6g059380 | 38.235 | 102 | 53 | 3 | 697 | 793 | 420 | 516 | 4.31e-12 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030163.t1 | AT5G48960 | 83.636 | 550 | 90 | 0 | 54 | 603 | 84 | 633 | 0.0 | 973 |
| MS.gene030163.t1 | AT1G75210 | 30.292 | 548 | 320 | 15 | 96 | 602 | 7 | 533 | 1.60e-66 | 234 |
| MS.gene030163.t1 | AT1G75210 | 30.292 | 548 | 320 | 15 | 96 | 602 | 7 | 533 | 1.60e-66 | 234 |
| MS.gene030163.t1 | AT1G75210 | 28.713 | 606 | 363 | 15 | 39 | 602 | 58 | 636 | 1.47e-65 | 234 |
| MS.gene030163.t1 | AT2G23890 | 27.623 | 467 | 295 | 13 | 131 | 588 | 5 | 437 | 3.52e-43 | 164 |
| MS.gene030163.t1 | AT2G23890 | 27.708 | 480 | 302 | 14 | 120 | 588 | 82 | 527 | 4.44e-43 | 166 |
| MS.gene030163.t1 | AT2G23890 | 26.733 | 505 | 300 | 14 | 120 | 588 | 82 | 552 | 6.75e-40 | 157 |
Find 286 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGTGAAATTCATGAATT+TGG | 0.129187 | 8.3:+5283417 | MS.gene030163:CDS |
| CAAGGTAAGGTTTCACTATA+AGG | 0.184972 | 8.3:+5283359 | MS.gene030163:CDS |
| TATGGAAAACCTTAAGAATA+TGG | 0.187472 | 8.3:+5275193 | MS.gene030163:CDS |
| TAGATTCCTTCCAAATGATA+TGG | 0.214715 | 8.3:+5276877 | MS.gene030163:CDS |
| GGTAAAATTAGAGTGTTAAA+TGG | 0.247733 | 8.3:+5284344 | MS.gene030163:CDS |
| AGAGTTACCTTTGGCACTTT+TGG | 0.268992 | 8.3:+5276690 | MS.gene030163:CDS |
| TTGATATAGTTGATGGTCTT+AGG | 0.278645 | 8.3:+5283544 | MS.gene030163:CDS |
| CTTGAACAGCTTGAGGCTTT+TGG | 0.288352 | 8.3:+5273625 | MS.gene030163:CDS |
| AGCTCGAGTAGATAACATTT+TGG | 0.302975 | 8.3:-5275442 | None:intergenic |
| AAAACTGCTGTGAGAGAGTA+TGG | 0.303499 | 8.3:+5284158 | MS.gene030163:CDS |
| CTGAAGGAAACCTGCGAGAT+AGG | 0.303944 | 8.3:+5282921 | MS.gene030163:CDS |
| AGGGTGAAATTCATGAATTT+GGG | 0.308458 | 8.3:+5283418 | MS.gene030163:CDS |
| CCAAGAAGCAGCAATGAAAA+TGG | 0.314560 | 8.3:+5282175 | MS.gene030163:CDS |
| TGACCTCTTTCCAGGTCTAT+AGG | 0.316104 | 8.3:+5284468 | MS.gene030163:intron |
| GAAGGAGTTGGTGATGGTGG+TGG | 0.319994 | 8.3:-5273428 | None:intergenic |
| CTACTAAATCTAATATGATA+TGG | 0.320134 | 8.3:-5284257 | None:intergenic |
| CTCTCCATGTTCGCCGTTCA+CGG | 0.333540 | 8.3:+5273391 | MS.gene030163:CDS |
| CTTGGTCCCCTTGATTATAA+AGG | 0.336443 | 8.3:+5276400 | MS.gene030163:CDS |
| TGATTATAAAGGTCTTTATA+AGG | 0.336485 | 8.3:+5276411 | MS.gene030163:CDS |
| CGACAAAGTGTCAGAGGTTT+GGG | 0.340066 | 8.3:-5273490 | None:intergenic |
| GACCTCTTTCCAGGTCTATA+GGG | 0.341857 | 8.3:+5284469 | MS.gene030163:intron |
| TAAATTGTTCAGGTAATTAG+AGG | 0.342453 | 8.3:+5275342 | MS.gene030163:intron |
| GAAAGTCGGAGTTGGTAATA+AGG | 0.347407 | 8.3:-5276820 | None:intergenic |
| CAGGGAAGCCCATATTCTTA+AGG | 0.349883 | 8.3:-5275202 | None:intergenic |
| TTGTCTTCTCATTCTTCTCT+AGG | 0.351969 | 8.3:-5284205 | None:intergenic |
| CATACATGGGGCTTGATGTT+TGG | 0.359037 | 8.3:-5277115 | None:intergenic |
| TTAGGTTGTTGAAATCTGAT+AGG | 0.365557 | 8.3:+5283562 | MS.gene030163:CDS |
| GAAACTGAATAGGTTTAAGT+TGG | 0.365732 | 8.3:+5284073 | MS.gene030163:CDS |
| GACTGACATCGGTGTAAATA+TGG | 0.369410 | 8.3:-5279608 | None:intergenic |
| GCTAAAAGACTTGGGCATTC+CGG | 0.369918 | 8.3:+5274238 | MS.gene030163:CDS |
| AAGGAGTTGGTGATGGTGGT+GGG | 0.374495 | 8.3:-5273427 | None:intergenic |
| CAAGGGTTGGGTTCACAGTC+TGG | 0.376985 | 8.3:-5283893 | None:intergenic |
| GTGATCTTTCAGAGCCTTAA+GGG | 0.379915 | 8.3:-5284420 | None:intergenic |
| TTCTGCAGGTGGTCTGTACT+CGG | 0.379962 | 8.3:+5279518 | MS.gene030163:intron |
| ACGACAAAGTGTCAGAGGTT+TGG | 0.381657 | 8.3:-5273491 | None:intergenic |
| CCAGGATTTGATATAGTTGA+TGG | 0.383496 | 8.3:+5283537 | MS.gene030163:CDS |
| TTGGTTAAAGCTGACCGATT+TGG | 0.384717 | 8.3:+5275393 | MS.gene030163:CDS |
| ATTTCAGATGGTTGACAGAT+TGG | 0.385670 | 8.3:+5276354 | MS.gene030163:intron |
| GATGAGGAAGGAAGTGTAGA+TGG | 0.387860 | 8.3:+5283810 | MS.gene030163:CDS |
| CTTCCAAACCAGTCACTTCA+AGG | 0.391341 | 8.3:-5283971 | None:intergenic |
| TTGCTGCTTCTTGGAGTTGA+AGG | 0.391965 | 8.3:-5282166 | None:intergenic |
| AAGGTAAGGTTTCACTATAA+GGG | 0.393065 | 8.3:+5283360 | MS.gene030163:CDS |
| AGTGATCTTTCAGAGCCTTA+AGG | 0.394844 | 8.3:-5284421 | None:intergenic |
| ATGCTGATATTTATACATCT+AGG | 0.395774 | 8.3:+5281369 | MS.gene030163:CDS |
| GACAATCTGATCAGAGAGAT+TGG | 0.397533 | 8.3:+5283480 | MS.gene030163:CDS |
| GTTGGAAAGCAATTGTGAAT+AGG | 0.398424 | 8.3:-5284320 | None:intergenic |
| CATATCATATTAGATTTAGT+AGG | 0.400907 | 8.3:+5284258 | MS.gene030163:CDS |
| ATGGAAAACCTTAAGAATAT+GGG | 0.403651 | 8.3:+5275194 | MS.gene030163:CDS |
| ACGCTGTTCGCCATCGCAAA+TGG | 0.405032 | 8.3:-5279641 | None:intergenic |
| GAACCTTGAAGTGACTGGTT+TGG | 0.405129 | 8.3:+5283968 | MS.gene030163:CDS |
| TTAGCAACGCTACGCTTTCA+AGG | 0.408899 | 8.3:+5282109 | MS.gene030163:CDS |
| TCAAATGCAAGTCCATCAAC+AGG | 0.409972 | 8.3:-5275221 | None:intergenic |
| TATAAAGACCTTTATAATCA+AGG | 0.414164 | 8.3:-5276408 | None:intergenic |
| TAGTTGGTGTTGAACAATCT+AGG | 0.415065 | 8.3:+5283946 | MS.gene030163:CDS |
| CAGAGAGATTGGGGTGGTAG+GGG | 0.415716 | 8.3:+5283491 | MS.gene030163:CDS |
| AATATGGGCTTCCCTGTTGA+TGG | 0.415915 | 8.3:+5275209 | MS.gene030163:CDS |
| ACAATCTGATCAGAGAGATT+GGG | 0.418266 | 8.3:+5283481 | MS.gene030163:CDS |
| AACCATAGTCAATCTTTAGT+TGG | 0.418562 | 8.3:+5283930 | MS.gene030163:CDS |
| TCCAATGCTGGAGGCGGATG+GGG | 0.419954 | 8.3:+5280988 | MS.gene030163:CDS |
| TTCTATCTCGCGCTGGACTG+TGG | 0.422171 | 8.3:+5281228 | MS.gene030163:CDS |
| TCTGCAGGTGGTCTGTACTC+GGG | 0.422238 | 8.3:+5279519 | MS.gene030163:intron |
| TCTCGCCAACCCATATCATT+TGG | 0.423439 | 8.3:-5276887 | None:intergenic |
| CACCAACTAAAGATTGACTA+TGG | 0.427200 | 8.3:-5283932 | None:intergenic |
| CTTGATGTTTGGAAGAACTC+TGG | 0.428049 | 8.3:-5277104 | None:intergenic |
| GTAAAATTAGAGTGTTAAAT+GGG | 0.428336 | 8.3:+5284345 | MS.gene030163:CDS |
| TCAGAGAGATTGGGGTGGTA+GGG | 0.431832 | 8.3:+5283490 | MS.gene030163:CDS |
| GAATCTGATCCTGATTATAA+TGG | 0.433976 | 8.3:+5283750 | MS.gene030163:CDS |
| AGTGTTAAATGGGAAGTTGT+TGG | 0.436088 | 8.3:+5284355 | MS.gene030163:CDS |
| TCCCCATCCGCCTCCAGCAT+TGG | 0.439169 | 8.3:-5280989 | None:intergenic |
| CTTCATGTATTTCCGCTCTC+AGG | 0.439716 | 8.3:+5281418 | MS.gene030163:CDS |
| TGTTATCTACTCGAGCTGTT+AGG | 0.441179 | 8.3:+5275448 | MS.gene030163:CDS |
| TCAGGCTCAACAAACTGTTC+AGG | 0.442004 | 8.3:-5276664 | None:intergenic |
| ATTCACAATTGCTTTCCAAC+TGG | 0.442066 | 8.3:+5284323 | MS.gene030163:CDS |
| GTATTTCCGCTCTCAGGAAC+AGG | 0.444670 | 8.3:+5281424 | MS.gene030163:CDS |
| TCCTCACTACCATTATAATC+AGG | 0.449699 | 8.3:-5283759 | None:intergenic |
| GCCAAAGGTAACTCTGGATC+AGG | 0.450953 | 8.3:-5276682 | None:intergenic |
| GTTGGGTTCACAGTCTGGTT+AGG | 0.453546 | 8.3:-5283888 | None:intergenic |
| TGATGAACCTGTAGTATTTG+AGG | 0.454625 | 8.3:+5284103 | MS.gene030163:CDS |
| TGGCACACCTTTCAGGCTGT+TGG | 0.456364 | 8.3:+5276497 | MS.gene030163:intron |
| GCCTGATCCAGAGTTACCTT+TGG | 0.457074 | 8.3:+5276681 | MS.gene030163:CDS |
| GACGTACCATCACATTATAA+TGG | 0.458097 | 8.3:-5274817 | None:intergenic |
| CTGCAGGTGGTCTGTACTCG+GGG | 0.458291 | 8.3:+5279520 | MS.gene030163:intron |
| TAGGTTCATAAATGAACATA+AGG | 0.461573 | 8.3:+5283593 | MS.gene030163:CDS |
| ATCACCTCGGCAACGAATCA+AGG | 0.462275 | 8.3:-5279849 | None:intergenic |
| AGATTCCTTCCAAATGATAT+GGG | 0.462351 | 8.3:+5276878 | MS.gene030163:CDS |
| CTGAGGTCAATTAGTGCCAT+TGG | 0.466336 | 8.3:+5274770 | MS.gene030163:CDS |
| GCCTGTCACTACTTCATACA+TGG | 0.467141 | 8.3:-5277129 | None:intergenic |
| CTCCAATGCTGGAGGCGGAT+GGG | 0.468100 | 8.3:+5280987 | MS.gene030163:CDS |
| CAAGAAGGAAGAAACTGAAT+AGG | 0.469248 | 8.3:+5284063 | MS.gene030163:CDS |
| GGGACCATACCGGCAGATCT+TGG | 0.471312 | 8.3:+5276382 | MS.gene030163:CDS |
| GGTAGAGCTTATAAATCAAA+AGG | 0.471884 | 8.3:+5279883 | MS.gene030163:CDS |
| AGACAAAGAGAGAGGGAACT+TGG | 0.471925 | 8.3:+5275374 | MS.gene030163:CDS |
| AGAGAACATATTACCAGATC+AGG | 0.473768 | 8.3:-5275245 | None:intergenic |
| CTGAATGACTACCAAGGGTT+GGG | 0.475262 | 8.3:-5283905 | None:intergenic |
| CCGAGGTGATAGAGAATCAT+TGG | 0.475672 | 8.3:+5279862 | MS.gene030163:CDS |
| CTCTCTCTTTGTCTATCACA+AGG | 0.475712 | 8.3:-5275365 | None:intergenic |
| ATGGGGAGCTCTTCAATTCA+AGG | 0.475973 | 8.3:+5281005 | MS.gene030163:CDS |
| ACTTGCATTTGATCCTGATC+TGG | 0.476464 | 8.3:+5275232 | MS.gene030163:CDS |
| CGAGAAAATTGCTCCAATGC+TGG | 0.480613 | 8.3:+5280976 | MS.gene030163:CDS |
| ATAATCCACGTCACACTTGT+TGG | 0.481051 | 8.3:-5273566 | None:intergenic |
| TAATGGTAGTGAGGAGGGAG+AGG | 0.481275 | 8.3:+5283767 | MS.gene030163:CDS |
| CATATTAGATTTAGTAGGGT+AGG | 0.485402 | 8.3:+5284263 | MS.gene030163:CDS |
| TTTGAAGGAGTTGGTGATGG+TGG | 0.486518 | 8.3:-5273431 | None:intergenic |
| TCTAATGCGTCCATGCTTCA+AGG | 0.486804 | 8.3:+5277157 | MS.gene030163:CDS |
| AGAAGATTTGCTAAAAGACT+TGG | 0.493046 | 8.3:+5274229 | MS.gene030163:CDS |
| ATAAAGACCTTTATAATCAA+GGG | 0.493255 | 8.3:-5276407 | None:intergenic |
| TTGGGCAAGTTGACATTGGT+GGG | 0.494282 | 8.3:-5273472 | None:intergenic |
| TAATCCACGTCACACTTGTT+GGG | 0.494358 | 8.3:-5273565 | None:intergenic |
| AGGTAGTTGTAGTTGACAAA+GGG | 0.496121 | 8.3:+5283694 | MS.gene030163:CDS |
| CCCATTGACTCTCCTTTCGC+AGG | 0.496558 | 8.3:-5275948 | None:intergenic |
| CCCTTCTTATAAGCTACCAC+AGG | 0.497448 | 8.3:-5283399 | None:intergenic |
| AAACTGAATAGGTTTAAGTT+GGG | 0.498217 | 8.3:+5284074 | MS.gene030163:CDS |
| AGAACCACTACTTCAAGGTA+AGG | 0.499341 | 8.3:+5283346 | MS.gene030163:CDS |
| TCTATCTCGCGCTGGACTGT+GGG | 0.500080 | 8.3:+5281229 | MS.gene030163:CDS |
| ATTGTGGTCAAGTGTAATCC+AGG | 0.501667 | 8.3:+5284230 | MS.gene030163:CDS |
| GCGTGCTTATGACTATTGTA+TGG | 0.501991 | 8.3:+5275175 | MS.gene030163:CDS |
| CCCTGTGGTAGCTTATAAGA+AGG | 0.503155 | 8.3:+5283398 | MS.gene030163:CDS |
| ACCTGCGAAAGGAGAGTCAA+TGG | 0.503886 | 8.3:+5275947 | MS.gene030163:CDS |
| ATAGTGACTTTGATGAAGAA+TGG | 0.505002 | 8.3:+5283847 | MS.gene030163:CDS |
| TCATGTTGAAGGTCGTCTTA+AGG | 0.505507 | 8.3:+5276538 | MS.gene030163:CDS |
| AAAAGTGCCAAAGGTAACTC+TGG | 0.509325 | 8.3:-5276688 | None:intergenic |
| CTAATATGATATGGACAACC+TGG | 0.510568 | 8.3:-5284248 | None:intergenic |
| AGAGCCTTAAGGGTCATAGT+AGG | 0.511754 | 8.3:-5284410 | None:intergenic |
| GAGGTAGTTGTAGTTGACAA+AGG | 0.512347 | 8.3:+5283693 | MS.gene030163:CDS |
| GGACCAAAACTTACTTGAGC+AGG | 0.513923 | 8.3:-5279968 | None:intergenic |
| GAGGGTCAGATATTTGCAAC+TGG | 0.514981 | 8.3:+5284125 | MS.gene030163:CDS |
| TAGTGACTTTGATGAAGAAT+GGG | 0.516770 | 8.3:+5283848 | MS.gene030163:CDS |
| ATAAATCAAAAGGAAGTTGT+GGG | 0.520344 | 8.3:+5279893 | MS.gene030163:CDS |
| TACGCCTACTATGACCCTTA+AGG | 0.521023 | 8.3:+5284406 | MS.gene030163:CDS |
| TATAAATCAAAAGGAAGTTG+TGG | 0.521533 | 8.3:+5279892 | MS.gene030163:CDS |
| TGAAGGTCCGATCGAGGAAG+TGG | 0.522632 | 8.3:+5274184 | MS.gene030163:CDS |
| TGAAGGAAACCTGCGAGATA+GGG | 0.522696 | 8.3:+5282922 | MS.gene030163:CDS |
| TGTTGAGAACCACTACTTCA+AGG | 0.522758 | 8.3:+5283341 | MS.gene030163:CDS |
| TTGTAGAGAACTTGAAGATG+AGG | 0.522798 | 8.3:+5279668 | MS.gene030163:CDS |
| GCTCCAATGCTGGAGGCGGA+TGG | 0.523158 | 8.3:+5280986 | MS.gene030163:CDS |
| TCCTTCCAAATGATATGGGT+TGG | 0.523213 | 8.3:+5276882 | MS.gene030163:CDS |
| TGCTGATATTTATACATCTA+GGG | 0.525292 | 8.3:+5281370 | MS.gene030163:CDS |
| CAAATGGTTGAAAATTCTCT+AGG | 0.528698 | 8.3:+5279552 | MS.gene030163:CDS |
| CCATCAACTATATCAAATCC+TGG | 0.529151 | 8.3:-5283537 | None:intergenic |
| AATCTGATAGGGATGTGGTT+AGG | 0.529153 | 8.3:+5283574 | MS.gene030163:CDS |
| ATCAGAGAGATTGGGGTGGT+AGG | 0.529321 | 8.3:+5283489 | MS.gene030163:CDS |
| GGGACCAAGATCTGCCGGTA+TGG | 0.529438 | 8.3:-5276386 | None:intergenic |
| TGAAGCAGTGGCCTACATGC+AGG | 0.529942 | 8.3:+5275999 | MS.gene030163:CDS |
| GAAGAAGGTGAGTACGAGTT+CGG | 0.531705 | 8.3:+5282835 | MS.gene030163:CDS |
| TTTGGGCAAGTTGACATTGG+TGG | 0.533529 | 8.3:-5273473 | None:intergenic |
| CGAGGAAGTGGCTAGAATAG+AGG | 0.533529 | 8.3:+5274196 | MS.gene030163:CDS |
| ATTGGTGATGCTGCATTTGA+AGG | 0.535973 | 8.3:+5274167 | MS.gene030163:CDS |
| TGAAAATAGAAAGTCGAAGA+AGG | 0.536416 | 8.3:+5282820 | MS.gene030163:CDS |
| GGACAAGACGAGTTCGAAGA+AGG | 0.536652 | 8.3:+5282856 | MS.gene030163:CDS |
| ATCCATGTTAGTTGCTGCAA+GGG | 0.537926 | 8.3:-5280897 | None:intergenic |
| TAGGTTGTTGAAATCTGATA+GGG | 0.538112 | 8.3:+5283563 | MS.gene030163:CDS |
| ACTTTGATGAAGAATGGGAT+TGG | 0.538471 | 8.3:+5283853 | MS.gene030163:CDS |
| GTATGATAGTGATGTTGAAG+AGG | 0.539772 | 8.3:+5283674 | MS.gene030163:CDS |
| ACTATTCGTTGGAAATGAAG+TGG | 0.540225 | 8.3:+5282795 | MS.gene030163:CDS |
| GAAAATTCTCTAGGAATCCA+TGG | 0.541230 | 8.3:+5279561 | MS.gene030163:CDS |
| TCTCTTTAACCAACTTCGAC+TGG | 0.541444 | 8.3:+5279919 | MS.gene030163:CDS |
| ATGTGGAAGTTGTTGACAGC+AGG | 0.541468 | 8.3:+5283652 | MS.gene030163:CDS |
| TGTTAAGCTTGAACAGCTTG+AGG | 0.541724 | 8.3:+5273618 | MS.gene030163:CDS |
| CCAGGTTGAGCCTTGAAGCA+TGG | 0.541981 | 8.3:-5277167 | None:intergenic |
| GTTCTCTGTGTCTGAAGCAG+TGG | 0.543616 | 8.3:+5275987 | MS.gene030163:CDS |
| TATGAATTAGCAGATGAGGA+AGG | 0.544031 | 8.3:+5283798 | MS.gene030163:CDS |
| TGCAATGGAAGTGAGATGTA+TGG | 0.544497 | 8.3:+5275910 | MS.gene030163:intron |
| AAGCTGTGGTATATATGTCC+AGG | 0.545924 | 8.3:+5283519 | MS.gene030163:CDS |
| CCATGCTTCAAGGCTCAACC+TGG | 0.546398 | 8.3:+5277167 | MS.gene030163:CDS |
| GACGCCGTGAACGGCGAACA+TGG | 0.546502 | 8.3:-5273395 | None:intergenic |
| AAGTCTTCATCAATGTCAGC+TGG | 0.546875 | 8.3:-5283723 | None:intergenic |
| TTTGCAGAGCCAGTCGAAGT+TGG | 0.547896 | 8.3:-5279928 | None:intergenic |
| TCTAGGAACCTTGAAGTGAC+TGG | 0.548530 | 8.3:+5283963 | MS.gene030163:CDS |
| TTTGTCTGTGTAGTGAAAGT+CGG | 0.549719 | 8.3:-5276834 | None:intergenic |
| ACAAGCAACGACGATGAAGA+AGG | 0.551463 | 8.3:+5282880 | MS.gene030163:CDS |
| AATTGCTCCAATGCTGGAGG+CGG | 0.551496 | 8.3:+5280982 | MS.gene030163:CDS |
| GACCACATCGACGCCGTGAA+CGG | 0.552605 | 8.3:-5273404 | None:intergenic |
| TGAGATGTATGGAAGAGAAC+TGG | 0.554032 | 8.3:+5275921 | MS.gene030163:CDS |
| TTATCTTGGTGAAAGCACTA+AGG | 0.555735 | 8.3:+5273585 | MS.gene030163:CDS |
| CGCCGTTCACGGCGTCGATG+TGG | 0.556998 | 8.3:+5273402 | MS.gene030163:CDS |
| CCATCACATTATAATGGACA+AGG | 0.557042 | 8.3:-5274811 | None:intergenic |
| TGACCCTCCTCAAATACTAC+AGG | 0.558010 | 8.3:-5284110 | None:intergenic |
| ATTCTAGCCACTTCCTCGAT+CGG | 0.558857 | 8.3:-5274191 | None:intergenic |
| CTATTCGTTGGAAATGAAGT+GGG | 0.559457 | 8.3:+5282796 | MS.gene030163:CDS |
| AATAATGAACTTTCTTCCAA+CGG | 0.564916 | 8.3:+5281592 | MS.gene030163:CDS |
| GAAGATTTGCTAAAAGACTT+GGG | 0.565532 | 8.3:+5274230 | MS.gene030163:CDS |
| CATACAAAATCTCATCTCCA+TGG | 0.565756 | 8.3:-5279578 | None:intergenic |
| ACTGAATGACTACCAAGGGT+TGG | 0.566556 | 8.3:-5283906 | None:intergenic |
| AGGAGTTGGTGATGGTGGTG+GGG | 0.566692 | 8.3:-5273426 | None:intergenic |
| ATATCATATTAGATTTAGTA+GGG | 0.566712 | 8.3:+5284259 | MS.gene030163:CDS |
| GTAGGCGTATGCTTAAGTAT+TGG | 0.568270 | 8.3:-5284392 | None:intergenic |
| TCAAGGGGACCAAGATCTGC+CGG | 0.568445 | 8.3:-5276391 | None:intergenic |
| GAAACCTTACCTTGAAGTAG+TGG | 0.569035 | 8.3:-5283350 | None:intergenic |
| CTGAGAGCGGAAATACATGA+AGG | 0.569482 | 8.3:-5281417 | None:intergenic |
| CCCATGTATGAAGTAGTGAC+AGG | 0.570886 | 8.3:+5277128 | MS.gene030163:CDS |
| TTCTGATCCAAAAGTGCCAA+AGG | 0.571904 | 8.3:-5276697 | None:intergenic |
| TGGGGATTTCTATCTCGCGC+TGG | 0.572308 | 8.3:+5281221 | MS.gene030163:CDS |
| CCTGCGAAAGGAGAGTCAAT+GGG | 0.572366 | 8.3:+5275948 | MS.gene030163:CDS |
| GATTATAATGGTAGTGAGGA+GGG | 0.572693 | 8.3:+5283762 | MS.gene030163:CDS |
| TAATTACCTGTTCCTGAGAG+CGG | 0.572727 | 8.3:-5281430 | None:intergenic |
| GACTTTAGATTGACTGACAT+CGG | 0.575353 | 8.3:-5279619 | None:intergenic |
| ATGGTTGACAGATTGGATGA+TGG | 0.575408 | 8.3:+5276361 | MS.gene030163:CDS |
| AAGTGTGACGTGGATTATCT+TGG | 0.576216 | 8.3:+5273571 | MS.gene030163:CDS |
| CACCACCAACCCTATCTCGC+AGG | 0.576568 | 8.3:-5282931 | None:intergenic |
| GACCCTTGCAGCAACTAACA+TGG | 0.577624 | 8.3:+5280895 | MS.gene030163:intron |
| ACGCGTGCAGAATATTCCAC+GGG | 0.578052 | 8.3:-5274738 | None:intergenic |
| CATCCATGTTAGTTGCTGCA+AGG | 0.578620 | 8.3:-5280898 | None:intergenic |
| GGCTAGAATAGAGGCTAGAG+AGG | 0.578970 | 8.3:+5274205 | MS.gene030163:CDS |
| TATCTTGGTGAAAGCACTAA+GGG | 0.583087 | 8.3:+5273586 | MS.gene030163:CDS |
| TGCAGGTGGTCTGTACTCGG+GGG | 0.584103 | 8.3:+5279521 | MS.gene030163:intron |
| CAATCTGATCAGAGAGATTG+GGG | 0.584420 | 8.3:+5283482 | MS.gene030163:CDS |
| GCACGCGTACGTTGAATCTG+AGG | 0.585348 | 8.3:+5274753 | MS.gene030163:CDS |
| AACCTGCGAGATAGGGTTGG+TGG | 0.586586 | 8.3:+5282929 | MS.gene030163:CDS |
| GGGTGAATTTCTAGATGAAG+AGG | 0.586989 | 8.3:-5274717 | None:intergenic |
| CTGGAACTATAGGCTTGGGA+AGG | 0.587407 | 8.3:+5275152 | MS.gene030163:intron |
| TGGTGCATCTGATGAAGAAG+AGG | 0.589233 | 8.3:+5284037 | MS.gene030163:CDS |
| GTTGAAATCTGATAGGGATG+TGG | 0.590072 | 8.3:+5283569 | MS.gene030163:CDS |
| GAATGAGAAGACAAGAATTG+TGG | 0.590708 | 8.3:+5284214 | MS.gene030163:CDS |
| TCATCTGCTAATTCATACTC+AGG | 0.591633 | 8.3:-5283792 | None:intergenic |
| CCTGTCACTACTTCATACAT+GGG | 0.592193 | 8.3:-5277128 | None:intergenic |
| GCCAACCCATATCATTTGGA+AGG | 0.592253 | 8.3:-5276883 | None:intergenic |
| ATTGGATGATGGGACCATAC+CGG | 0.592630 | 8.3:+5276372 | MS.gene030163:CDS |
| TTATGTGGAATCTAAAGATG+TGG | 0.592947 | 8.3:+5283635 | MS.gene030163:CDS |
| CAGACTGTGAACCCAACCCT+TGG | 0.593647 | 8.3:+5283894 | MS.gene030163:CDS |
| AGAACTGGTGGACCTGCGAA+AGG | 0.593804 | 8.3:+5275936 | MS.gene030163:CDS |
| TGAGTATGAATTAGCAGATG+AGG | 0.594067 | 8.3:+5283794 | MS.gene030163:CDS |
| TAAAGACCTTTATAATCAAG+GGG | 0.594282 | 8.3:-5276406 | None:intergenic |
| CCTTGTCCATTATAATGTGA+TGG | 0.594817 | 8.3:+5274811 | MS.gene030163:CDS |
| AATGAACATAAGGATGCAGT+AGG | 0.595264 | 8.3:+5283603 | MS.gene030163:CDS |
| TGGTTGACAGATTGGATGAT+GGG | 0.595425 | 8.3:+5276362 | MS.gene030163:CDS |
| TGTGTAGTGAAAGTCGGAGT+TGG | 0.598547 | 8.3:-5276828 | None:intergenic |
| TGGAACTATAGGCTTGGGAA+GGG | 0.601614 | 8.3:+5275153 | MS.gene030163:intron |
| TCTTCTTCATCAGATGCACC+AGG | 0.603097 | 8.3:-5284035 | None:intergenic |
| AGGTTTGGGCAAGTTGACAT+TGG | 0.603601 | 8.3:-5273476 | None:intergenic |
| GGAGAAAACATCACCGCCGA+TGG | 0.604635 | 8.3:-5273533 | None:intergenic |
| TGATTATAATGGTAGTGAGG+AGG | 0.605286 | 8.3:+5283761 | MS.gene030163:CDS |
| GGTGAATTTCTAGATGAAGA+GGG | 0.605541 | 8.3:-5274716 | None:intergenic |
| AGCGTTGACTCTAAGTCCAT+CGG | 0.606577 | 8.3:+5273517 | MS.gene030163:CDS |
| ATGAAGAAGAGGGATCAAGA+AGG | 0.608295 | 8.3:+5284048 | MS.gene030163:CDS |
| AACTTCGACTGGCTCTGCAA+AGG | 0.608705 | 8.3:+5279930 | MS.gene030163:CDS |
| CCTGTGGTAGCTTATAAGAA+GGG | 0.610930 | 8.3:+5283399 | MS.gene030163:CDS |
| ATAGTCCATGTCATATCCAA+TGG | 0.613622 | 8.3:-5274786 | None:intergenic |
| ACGGAATCAACAAGAACTGA+AGG | 0.614901 | 8.3:+5282905 | MS.gene030163:CDS |
| TCAGATATACTAGAAAGTCC+TGG | 0.616594 | 8.3:+5284017 | MS.gene030163:CDS |
| ACACTTGTTGGGAGGCGTGA+CGG | 0.618790 | 8.3:-5273554 | None:intergenic |
| GCCTCCTCACATGTATGCAA+CGG | 0.622993 | 8.3:+5273363 | None:intergenic |
| GTTGCAACATACCTGCATGT+AGG | 0.623453 | 8.3:-5276010 | None:intergenic |
| TACGCGTGCAGAATATTCCA+CGG | 0.624452 | 8.3:-5274739 | None:intergenic |
| TAGTGCCATTGGATATGACA+TGG | 0.626673 | 8.3:+5274781 | MS.gene030163:CDS |
| TATGAAGTAGTGACAGGCGA+GGG | 0.628014 | 8.3:+5277134 | MS.gene030163:CDS |
| GGAAACCTGCGAGATAGGGT+TGG | 0.630181 | 8.3:+5282926 | MS.gene030163:CDS |
| GTTAACTGAATGACTACCAA+GGG | 0.630808 | 8.3:-5283910 | None:intergenic |
| AATCTAAAGTCCATTTGCGA+TGG | 0.631481 | 8.3:+5279631 | MS.gene030163:CDS |
| TGAACCTGTAGTATTTGAGG+AGG | 0.632760 | 8.3:+5284106 | MS.gene030163:CDS |
| TTACTTCATTTGTGACCCTG+TGG | 0.633469 | 8.3:+5283383 | MS.gene030163:CDS |
| GGGTGAAATTCATGAATTTG+GGG | 0.637450 | 8.3:+5283419 | MS.gene030163:CDS |
| CAAGCAACGACGATGAAGAA+GGG | 0.637650 | 8.3:+5282881 | MS.gene030163:CDS |
| GCCTCCCAACAAGTGTGACG+TGG | 0.643903 | 8.3:+5273561 | MS.gene030163:CDS |
| TGCATTTGAAGGTCCGATCG+AGG | 0.645835 | 8.3:+5274178 | MS.gene030163:CDS |
| AGTGCCTTGATTCGTTGCCG+AGG | 0.647545 | 8.3:+5279845 | MS.gene030163:CDS |
| GAAAATTGCTCCAATGCTGG+AGG | 0.647752 | 8.3:+5280979 | MS.gene030163:CDS |
| CTGCAACGACAAAGTGTCAG+AGG | 0.648717 | 8.3:-5273496 | None:intergenic |
| TCCTGATTATAATGGTAGTG+AGG | 0.650374 | 8.3:+5283758 | MS.gene030163:CDS |
| TGAGAGCGGAAATACATGAA+GGG | 0.652149 | 8.3:-5281416 | None:intergenic |
| CTTGTGATAGACAAAGAGAG+AGG | 0.653071 | 8.3:+5275366 | MS.gene030163:CDS |
| TTGTGATAGACAAAGAGAGA+GGG | 0.657865 | 8.3:+5275367 | MS.gene030163:CDS |
| GGTTAACTGAATGACTACCA+AGG | 0.658634 | 8.3:-5283911 | None:intergenic |
| GGGTGTGCGGAACACTGATG+AGG | 0.660718 | 8.3:+5283440 | MS.gene030163:CDS |
| GTGAATTTCTAGATGAAGAG+GGG | 0.660916 | 8.3:-5274715 | None:intergenic |
| GGTGCATCTGATGAAGAAGA+GGG | 0.664118 | 8.3:+5284038 | MS.gene030163:CDS |
| TCCACGTCACACTTGTTGGG+AGG | 0.666845 | 8.3:-5273562 | None:intergenic |
| TCATCTAGAAATTCACCCCG+TGG | 0.668363 | 8.3:+5274722 | MS.gene030163:CDS |
| GGTGAAATTCATGAATTTGG+GGG | 0.672461 | 8.3:+5283420 | MS.gene030163:CDS |
| GAACCTGTAGTATTTGAGGA+GGG | 0.674004 | 8.3:+5284107 | MS.gene030163:CDS |
| GCAGGTGGTCTGTACTCGGG+GGG | 0.677124 | 8.3:+5279522 | MS.gene030163:intron |
| AACGACGATGAAGAAGGGTA+CGG | 0.677886 | 8.3:+5282886 | MS.gene030163:CDS |
| CCAATGATTCTCTATCACCT+CGG | 0.680632 | 8.3:-5279862 | None:intergenic |
| GTGTGTATGAATCGTGTGCG+AGG | 0.682093 | 8.3:-5281552 | None:intergenic |
| GTATGAAGTAGTGACAGGCG+AGG | 0.683636 | 8.3:+5277133 | MS.gene030163:CDS |
| CAAATGCAAGTCCATCAACA+GGG | 0.684639 | 8.3:-5275220 | None:intergenic |
| CTGTCACTACTTCATACATG+GGG | 0.689870 | 8.3:-5277127 | None:intergenic |
| GATGTATGGAAGAGAACTGG+TGG | 0.697320 | 8.3:+5275924 | MS.gene030163:CDS |
| CATCACATTATAATGGACAA+GGG | 0.698479 | 8.3:-5274810 | None:intergenic |
| ACTACACAGACAAAATGATG+AGG | 0.704274 | 8.3:+5276843 | MS.gene030163:CDS |
| CCTTCTTATAAGCTACCACA+GGG | 0.705463 | 8.3:-5283398 | None:intergenic |
| CAAAACTTACTTGAGCAGGA+CGG | 0.709767 | 8.3:-5279964 | None:intergenic |
| GTTGACTCTAAGTCCATCGG+CGG | 0.719722 | 8.3:+5273520 | MS.gene030163:CDS |
| TGGTAGGGGAGTACAAGCTG+TGG | 0.722338 | 8.3:+5283505 | MS.gene030163:CDS |
| TCTGATCAGAGAGATTGGGG+TGG | 0.753650 | 8.3:+5283485 | MS.gene030163:CDS |
| AGTCTGGTTAGGCAAAACAG+TGG | 0.762941 | 8.3:-5283877 | None:intergenic |
| CGCGTGCAGAATATTCCACG+GGG | 0.772588 | 8.3:-5274737 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 5273373 | 5284489 | 5273373 | ID=MS.gene030163 |
| chr8.3 | mRNA | 5273373 | 5284489 | 5273373 | ID=MS.gene030163.t1;Parent=MS.gene030163 |
| chr8.3 | exon | 5273373 | 5273646 | 5273373 | ID=MS.gene030163.t1.exon1;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5273373 | 5273646 | 5273373 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5274165 | 5274259 | 5274165 | ID=MS.gene030163.t1.exon2;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5274165 | 5274259 | 5274165 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5274713 | 5274832 | 5274713 | ID=MS.gene030163.t1.exon3;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5274713 | 5274832 | 5274713 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5275164 | 5275253 | 5275164 | ID=MS.gene030163.t1.exon4;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5275164 | 5275253 | 5275164 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5275354 | 5275469 | 5275354 | ID=MS.gene030163.t1.exon5;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5275354 | 5275469 | 5275354 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5275921 | 5276020 | 5275921 | ID=MS.gene030163.t1.exon6;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5275921 | 5276020 | 5275921 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5276361 | 5276432 | 5276361 | ID=MS.gene030163.t1.exon7;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5276361 | 5276432 | 5276361 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5276512 | 5276559 | 5276512 | ID=MS.gene030163.t1.exon8;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5276512 | 5276559 | 5276512 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5276646 | 5276723 | 5276646 | ID=MS.gene030163.t1.exon9;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5276646 | 5276723 | 5276646 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5276803 | 5276922 | 5276803 | ID=MS.gene030163.t1.exon10;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5276803 | 5276922 | 5276803 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5277083 | 5277188 | 5277083 | ID=MS.gene030163.t1.exon11;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5277083 | 5277188 | 5277083 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5279526 | 5279689 | 5279526 | ID=MS.gene030163.t1.exon12;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5279526 | 5279689 | 5279526 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5279842 | 5279976 | 5279842 | ID=MS.gene030163.t1.exon13;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5279842 | 5279976 | 5279842 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5280896 | 5281026 | 5280896 | ID=MS.gene030163.t1.exon14;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5280896 | 5281026 | 5280896 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5281220 | 5281282 | 5281220 | ID=MS.gene030163.t1.exon15;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5281220 | 5281282 | 5281220 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5281367 | 5281445 | 5281367 | ID=MS.gene030163.t1.exon16;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5281367 | 5281445 | 5281367 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5281550 | 5281629 | 5281550 | ID=MS.gene030163.t1.exon17;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5281550 | 5281629 | 5281550 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5282081 | 5282198 | 5282081 | ID=MS.gene030163.t1.exon18;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5282081 | 5282198 | 5282081 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5282782 | 5282969 | 5282782 | ID=MS.gene030163.t1.exon19;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5282782 | 5282969 | 5282782 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5283338 | 5284443 | 5283338 | ID=MS.gene030163.t1.exon20;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5283338 | 5284443 | 5283338 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| chr8.3 | exon | 5284482 | 5284489 | 5284482 | ID=MS.gene030163.t1.exon21;Parent=MS.gene030163.t1 |
| chr8.3 | CDS | 5284482 | 5284489 | 5284482 | ID=cds.MS.gene030163.t1;Parent=MS.gene030163.t1 |
| Gene Sequence |
| Protein sequence |
