AlfalfaGEDB | MS.gene030254

M. sativa cultivar XinJiangDaYe / MS.gene030254

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF12854.10 PF13041.9 PF20431.1

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030254.t1	XP_013453260.1	94.2	464	27	0	1	464	145	608	1.80E-213	751.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030254.t1	Q9C866	59.7	452	181	1	1	452	88	538	6.1e-122	439.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030254.t1	A0A072UBU1	94.2	464	27	0	1	464	145	608	1.3e-213	751.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002733	MS.gene030254	PPI
MS.gene004420	MS.gene030254	PPI
MS.gene030254	MS.gene86533	PPI
MS.gene030254	MS.gene86539	PPI
MS.gene030254	MS.gene037019	PPI
MS.gene030254	MS.gene56014	PPI
MS.gene030254	MS.gene29389	PPI
MS.gene030254	MS.gene00186	PPI
MS.gene030254	MS.gene042299	PPI
MS.gene030254	MS.gene022333	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030254.t1	MTR_6g093170	94.181	464	27	0	1	464	145	608	0.0	881
MS.gene030254.t1	MTR_6g093170	29.467	319	161	5	28	314	71	357	1.14e-34	137
MS.gene030254.t1	MTR_6g093170	21.505	279	210	4	149	426	59	329	4.25e-17	84.3
MS.gene030254.t1	MTR_4g024860	42.266	459	261	2	1	459	200	654	2.73e-131	404
MS.gene030254.t1	MTR_4g024860	23.136	389	236	5	10	367	107	463	3.03e-30	125
MS.gene030254.t1	MTR_4g024860	25.197	254	170	6	142	387	106	347	1.19e-14	77.0
MS.gene030254.t1	MTR_7g093900	41.667	444	254	3	1	443	186	625	2.58e-124	376
MS.gene030254.t1	MTR_7g093900	23.604	394	224	8	5	360	89	443	2.52e-30	124
MS.gene030254.t1	MTR_7g093900	41.667	444	254	3	1	443	136	575	8.63e-122	375
MS.gene030254.t1	MTR_7g093900	23.664	393	223	8	5	359	39	392	6.48e-30	124

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030254.t1	AT1G31430	58.186	452	188	1	1	452	88	538	0.0	561
MS.gene030254.t1	AT1G31430	25.078	319	174	6	28	314	14	299	5.83e-31	125
MS.gene030254.t1	AT1G31430	23.741	278	202	5	155	431	8	276	3.84e-16	81.3
MS.gene030254.t1	AT1G31430	58.186	452	188	1	1	452	88	538	0.0	561
MS.gene030254.t1	AT1G31430	25.078	319	174	6	28	314	14	299	5.83e-31	125
MS.gene030254.t1	AT1G31430	23.741	278	202	5	155	431	8	276	3.84e-16	81.3
MS.gene030254.t1	AT2G22410	42.568	444	251	2	1	444	198	637	1.07e-130	394
MS.gene030254.t1	AT2G22410	24.051	395	235	4	10	371	103	465	4.28e-34	135
MS.gene030254.t1	AT2G22410	23.510	302	217	5	95	387	49	345	1.12e-18	89.4
MS.gene030254.t1	AT3G15930	40.175	458	268	3	3	459	179	631	1.21e-123	375

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Find 101 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCCCCAACTCCTCCC+TGG	0.548474	6.2:-89378401	None:intergenic
AAACAACTCCAGTGCTTCGC+TGG	0.480403	6.2:-89377887	None:intergenic
AACTTATGGCAATACTGATA+TGG	0.265929	6.2:+89378191	MS.gene030254:CDS
AATACCATATATACATGGCA+TGG	0.562254	6.2:-89378019	None:intergenic
ACAAATAATCGAAGTCCACG+AGG	0.678490	6.2:-89377842	None:intergenic
ACAATAAAAGTAACATCATC+AGG	0.443346	6.2:-89377937	None:intergenic
ACAGTCCAGCTCGACCAAGA+AGG	0.613569	6.2:-89378077	None:intergenic
ACCTCAATGGCACTACATCC+TGG	0.530998	6.2:-89378363	None:intergenic
ACGTGAAAACAGAATCGTGG+TGG	0.665326	6.2:+89377720	MS.gene030254:CDS
ACTTATGGCAATACTGATAT+GGG	0.475237	6.2:+89378192	MS.gene030254:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTTATGGCAATACTGATA+TGG	+	chr6.2:89378191-89378210	MS.gene030254:CDS	30.0%
AATACCATATATACATGGCA+TGG	-	chr6.2:89378022-89378041	None:intergenic	30.0%
ACTTATGGCAATACTGATAT+GGG	+	chr6.2:89378192-89378211	MS.gene030254:CDS	30.0%
GAAAGATTTGCATATCAGAA+AGG	+	chr6.2:89378338-89378357	MS.gene030254:CDS	30.0%
TCATGGAATGTTATGATATC+TGG	+	chr6.2:89377142-89377161	MS.gene030254:CDS	30.0%
TTGGATATGTATTGTAAGTG+TGG	+	chr6.2:89377370-89377389	MS.gene030254:CDS	30.0%
ATGGATATGTATGCTCAACT+GGG	+	chr6.2:89377064-89377083	MS.gene030254:CDS	35.0%
CATTCCATGCCATGTATATA+TGG	+	chr6.2:89378015-89378034	MS.gene030254:CDS	35.0%
CCATGAAACATTATCTCTCA+CGG	-	chr6.2:89377128-89377147	None:intergenic	35.0%
GAAGCCTGATAAGTTTATTG+TGG	+	chr6.2:89377630-89377649	MS.gene030254:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	89377064	89378458	89377064	ID=MS.gene030254
chr6.2	mRNA	89377064	89378458	89377064	ID=MS.gene030254.t1;Parent=MS.gene030254
chr6.2	exon	89377064	89378458	89377064	ID=MS.gene030254.t1.exon1;Parent=MS.gene030254.t1
chr6.2	CDS	89377064	89378458	89377064	ID=cds.MS.gene030254.t1;Parent=MS.gene030254.t1

Gene Sequence

>MS.gene030254

ATGGATATGTATGCTCAACTGGGTCGGATTGATTTTGTACGTAAACTGTTTGATGAAATGTCCGTGAGAGATAATGTTTCATGGAATGTTATGATATCTGGTTGTGTTAAGTGTAGGAGATTTCAAGAAGCCGTGGAAGTTTTTCAACGCATGAGGGTTGAAGGTAATGTGATGATTAGTGAAGCTACTGTTGTTAGTAGTTTGACTGCGTGTGCTGCGTTGAGGAATGTGGAGGTTGGGAAAGAGATTCATGGTTTTATAATTGAAAAGGAATTGGATTTTACGATGATAATGGGGAATGCGTTGTTGGATATGTATTGTAAGTGTGGTTATGTGAATGTGGCTCGTGAGATTTTTGATGGGATGATTGAGAGGAATGTGAATTGTTGGACTAGTATGGTGACTGGTTATGTGAGTTGTGGTGAGTTGGATAAAGCGAGAGATTTGTTTGATAAGAGTCCGACGAGGGATGTCGTTCTTTGGACGGCTATGATTAATGGGTATGTGCAGTTTAATCGTTTTGATGAGGCGGTTACGTTGTTTGAGGAGATGCAAGTTAGAGGAGTGAAGCCTGATAAGTTTATTGTGGTTGCTCTTCTTACGTGTTGTGCGCAGTTGGGAACTTTAGAGCATGGAAGGTGGATTCATGATTATATACGTGAAAACAGAATCGTGGTGGATGCTGTGGTTGGTACTTCTCTTATTGAAATGTATGCTAAATGTGGTTGTGTAGAGAAATCTTTGGAGGTTTTTATTGGATTGAAGGAAAAGGATACTGCCTCGTGGACTTCGATTATTTGTGGACTGGCCATGAATGGTAAGACCAGCGAAGCACTGGAGTTGTTTGAAGAAATGAAACTATTTGGTGCAAAACCTGATGATGTTACTTTTATTGTTCTTTTGAATGCGTGTAGTCATGGAGGATTGGTAGAAGAAGGCCGCAAGTTATTTCATTCCATGCCATGTATATATGGTATTGAGCCAAATTTAGAACACTATGGGTGTTTCATCGACCTTCTTGGTCGAGCTGGACTGTTATGTGAGGCGGAGGAGTTGATAAGGAAGTTGCCGGACCAAAAAAATGAGACAACAGTTGCAATTTATGGATCTTTGCTTAGTGCCTGCAGAACTTATGGCAATACTGATATGGGTGAAAGGATTGCTTCAGCACTAGCAAAAGTGAAATCCAGCGATTCGAGTCTTCATTCACTCCTTGCTAGCATTTATGCTTCTGCTGACAGATGGGAAGATGCAAGCAAAACGAGAAGTAAGATGAAAGATTTGCATATCAGAAAGGTGCCAGGATGTAGTGCCATTGAGGTGGATGGCATTGGAAACCAGGGAGGAGTTGGGGACTTTTCCAGTTTTCGGTCCAAAATTGGTCTGCCTACATGA

Protein sequence

>MS.gene030254.t1

MDMYAQLGRIDFVRKLFDEMSVRDNVSWNVMISGCVKCRRFQEAVEVFQRMRVEGNVMISEATVVSSLTACAALRNVEVGKEIHGFIIEKELDFTMIMGNALLDMYCKCGYVNVAREIFDGMIERNVNCWTSMVTGYVSCGELDKARDLFDKSPTRDVVLWTAMINGYVQFNRFDEAVTLFEEMQVRGVKPDKFIVVALLTCCAQLGTLEHGRWIHDYIRENRIVVDAVVGTSLIEMYAKCGCVEKSLEVFIGLKEKDTASWTSIICGLAMNGKTSEALELFEEMKLFGAKPDDVTFIVLLNACSHGGLVEEGRKLFHSMPCIYGIEPNLEHYGCFIDLLGRAGLLCEAEELIRKLPDQKNETTVAIYGSLLSACRTYGNTDMGERIASALAKVKSSDSSLHSLLASIYASADRWEDASKTRSKMKDLHIRKVPGCSAIEVDGIGNQGGVGDFSSFRSKIGLPT