AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033616


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033616.t1 MTR_7g082540 75.191 262 59 4 1 261 1 257 2.98e-138 389
MS.gene033616.t1 MTR_7g082390 75.391 256 58 4 1 255 1 252 7.45e-136 383
MS.gene033616.t1 MTR_7g082533 34.956 226 127 6 35 243 36 258 1.75e-36 131
MS.gene033616.t1 MTR_7g082537 30.303 264 157 13 11 259 12 263 1.86e-28 109
MS.gene033616.t1 MTR_7g082530 31.878 229 145 7 25 244 27 253 4.19e-25 104
MS.gene033616.t1 MTR_7g082530 31.878 229 145 7 25 244 27 253 4.38e-25 104
MS.gene033616.t1 MTR_7g082460 29.555 247 142 10 17 243 21 255 1.45e-24 102
MS.gene033616.t1 MTR_6g082940 29.119 261 163 10 1 242 1 258 3.49e-24 99.8
MS.gene033616.t1 MTR_6g082920 29.119 261 163 10 1 242 1 258 3.49e-24 99.8
MS.gene033616.t1 MTR_8g104520 32.653 245 139 9 16 239 5 244 3.70e-24 101
MS.gene033616.t1 MTR_7g082470 27.612 268 148 11 19 254 1 254 3.64e-22 95.9
MS.gene033616.t1 MTR_6g082820 29.084 251 153 10 11 242 14 258 7.85e-22 92.8
MS.gene033616.t1 MTR_6g083000 30.493 223 136 9 39 245 43 262 1.97e-20 89.4
MS.gene033616.t1 MTR_1g115485 28.137 263 160 12 17 255 8 265 3.47e-20 90.1
MS.gene033616.t1 MTR_7g082510 29.583 240 129 13 17 241 21 235 1.67e-17 82.0
MS.gene033616.t1 MTR_6g082990 29.577 213 137 9 41 243 35 244 7.87e-17 78.2
MS.gene033616.t1 MTR_6g082980 33.766 154 88 6 16 158 17 167 8.28e-16 75.5
MS.gene033616.t1 MTR_7g082430 28.500 200 101 9 35 227 41 205 3.13e-14 72.4
MS.gene033616.t1 MTR_6g044830 31.683 202 120 11 39 226 4 201 1.35e-13 70.5
MS.gene033616.t1 MTR_6g083020 27.119 236 154 11 17 243 24 250 4.17e-13 69.3
MS.gene033616.t1 MTR_7g082500 33.898 118 66 5 34 140 10 126 5.69e-13 65.5
MS.gene033616.t1 MTR_6g083010 28.070 228 130 9 13 219 23 237 5.74e-13 67.8
MS.gene033616.t1 MTR_6g083760 26.482 253 157 13 14 246 8 251 5.42e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033616.t1 AT1G66880 33.498 203 119 7 35 233 33 223 1.10e-25 106
MS.gene033616.t1 AT1G66930 32.900 231 125 10 35 254 33 244 1.23e-25 106
MS.gene033616.t1 AT1G66930 27.016 248 147 9 26 253 243 476 2.32e-14 73.2
MS.gene033616.t1 AT1G66880 33.498 203 119 7 35 233 33 223 1.36e-25 105
MS.gene033616.t1 AT1G66880 25.112 223 141 9 35 238 252 467 2.20e-13 70.1
MS.gene033616.t1 AT1G66880 33.166 199 117 7 39 233 416 602 2.77e-24 102
MS.gene033616.t1 AT1G66880 28.351 194 125 6 41 231 26 208 1.68e-14 73.6
MS.gene033616.t1 AT1G66880 25.112 223 141 9 35 238 631 846 2.14e-13 70.5
MS.gene033616.t1 AT1G67000 33.457 269 138 13 5 262 3 241 9.97e-24 100
MS.gene033616.t1 AT1G67000 29.719 249 136 11 25 247 241 476 4.11e-18 84.3
MS.gene033616.t1 AT5G38260 29.508 244 154 9 14 242 12 252 2.26e-23 99.4
MS.gene033616.t1 AT5G38210 30.769 221 136 8 16 229 16 226 3.06e-23 99.0
MS.gene033616.t1 AT1G66920 32.338 201 121 5 35 231 33 222 1.46e-21 94.0
MS.gene033616.t1 AT1G66920 32.338 201 121 5 35 231 33 222 1.56e-21 94.0
MS.gene033616.t1 AT1G66920 32.338 201 121 5 35 231 33 222 1.78e-21 94.0
MS.gene033616.t1 AT5G38210 27.869 244 149 10 18 242 16 251 1.55e-20 91.3
MS.gene033616.t1 AT1G66940 31.250 272 130 13 8 241 4 256 2.72e-20 88.6
MS.gene033616.t1 AT1G66940 31.250 272 130 13 8 241 4 256 2.72e-20 88.6
MS.gene033616.t1 AT1G66940 31.203 266 138 12 8 241 4 256 3.23e-20 88.6
MS.gene033616.t1 AT5G38210 27.027 222 140 8 35 242 20 233 4.31e-20 89.7
MS.gene033616.t1 AT1G66940 31.203 266 138 12 8 241 4 256 8.02e-20 87.8
MS.gene033616.t1 AT5G38250 30.702 228 127 11 25 237 28 239 2.95e-16 78.6
MS.gene033616.t1 AT1G66910 28.000 250 146 10 16 238 16 258 3.33e-16 78.6
MS.gene033616.t1 AT1G66910 28.000 250 146 10 16 238 16 258 4.59e-16 78.2
MS.gene033616.t1 AT5G38240 33.679 193 106 9 26 208 26 206 9.04e-14 71.2
MS.gene033616.t1 AT5G38240 33.679 193 106 9 26 208 26 206 9.55e-14 71.2
MS.gene033616.t1 AT1G66930 26.432 227 139 8 26 232 62 280 3.66e-11 63.5
MS.gene033616.t1 AT1G66930 26.432 227 139 8 26 232 62 280 4.16e-11 63.2
MS.gene033616.t1 AT1G18390 26.693 251 144 12 25 252 18 251 7.84e-11 62.4

Find 53 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACACCGACCTCTTTGTTTA+TGG 0.138942 7.4:+24318495 MS.gene033616:CDS
CCAGAACAACCGTGCTTTCT+TGG 0.164666 7.4:-24318671 None:intergenic
ATTCTCATAACATGTAAATT+AGG 0.184326 7.4:-24318411 None:intergenic
ACTTGGTTTGGTTCTAATAT+TGG 0.259610 7.4:+24318630 MS.gene033616:CDS
TGTGGAACTATAAAACAAAT+TGG 0.275223 7.4:+24318264 MS.gene033616:CDS
GGATTATGAATTGAAGAATA+TGG 0.300665 7.4:-24318176 None:intergenic
CCAAGAAAGCACGGTTGTTC+TGG 0.326952 7.4:+24318671 MS.gene033616:CDS
TGAAGTCAAGTATCTGGTTT+TGG 0.330615 7.4:+24318380 MS.gene033616:CDS
AAAGGGTGGCCACACTCTTT+TGG 0.336772 7.4:-24318312 None:intergenic
TTCTCATAACATGTAAATTA+GGG 0.352436 7.4:-24318410 None:intergenic
AATCAATGAAGTCAAGTATC+TGG 0.353721 7.4:+24318374 MS.gene033616:CDS
CTGATGAATATGTTTATACT+TGG 0.407475 7.4:+24318613 MS.gene033616:CDS
TTCATTGATTGTTATGTAGC+TGG 0.420003 7.4:-24318361 None:intergenic
GAGCTTCATTAGAACAAGAT+TGG 0.423731 7.4:-24318866 None:intergenic
TGCAATTGATATTCAACAAA+GGG 0.426809 7.4:-24318329 None:intergenic
GAATATGTTTATACTTGGTT+TGG 0.429857 7.4:+24318618 MS.gene033616:CDS
AATTGAAGAATATGGAAAGT+AGG 0.454150 7.4:-24318168 None:intergenic
ATTGAAGAATATGGAAAGTA+GGG 0.482727 7.4:-24318167 None:intergenic
TTCTTGCAAATAGTCCTCTC+TGG 0.490100 7.4:-24318436 None:intergenic
TCATCAGAAGAACCATTTGA+AGG 0.497784 7.4:-24318597 None:intergenic
AGATTGGTCTCCACAGTAGC+AGG 0.507693 7.4:-24318850 None:intergenic
CATAACATGTAAATTAGGGC+TGG 0.521705 7.4:-24318406 None:intergenic
TCATATTTGAGCTCCGATGA+AGG 0.522630 7.4:+24318222 MS.gene033616:CDS
GGTGGAGATTGCTGAGACTG+TGG 0.523150 7.4:-24318888 None:intergenic
TGTGAGAATCTATTTAATTG+TGG 0.523585 7.4:+24318246 MS.gene033616:CDS
AAGCAAACAACCTGCTACTG+TGG 0.532581 7.4:+24318840 MS.gene033616:CDS
TAGATTCTCACAACCTTCAT+CGG 0.538981 7.4:-24318235 None:intergenic
GGTCGATCCCCTCCCCAGAA+AGG 0.542122 7.4:-24318291 None:intergenic
GGCGAAGGGAAAGTAATTGA+AGG 0.551078 7.4:-24318555 None:intergenic
TCATCGGAGCTCAAATATGA+AGG 0.558393 7.4:-24318219 None:intergenic
TTTGTGGTGAGATGGATAGC+TGG 0.564260 7.4:+24318756 MS.gene033616:CDS
ACTTTAGACATACCTGGAGG+TGG 0.566661 7.4:-24318906 None:intergenic
TGCAATCAGAGATGGATTTG+TGG 0.566962 7.4:+24318740 MS.gene033616:CDS
GTACAAAGTGCAATCAGAGA+TGG 0.568148 7.4:+24318732 MS.gene033616:CDS
TCTCAGCAATCTCCACCTCC+AGG 0.572777 7.4:+24318894 MS.gene033616:CDS
GTTTACACTTTAGACATACC+TGG 0.572898 7.4:-24318912 None:intergenic
AAAAGTGTCCGCTAGGCGAA+GGG 0.575500 7.4:-24318569 None:intergenic
GTGCAATTGATATTCAACAA+AGG 0.577816 7.4:-24318330 None:intergenic
GAGATGGATTTGTGGTGAGA+TGG 0.580019 7.4:+24318748 MS.gene033616:CDS
TGTTTAAACCATAAACAAAG+AGG 0.580084 7.4:-24318503 None:intergenic
AGTAGGGAGGATATTAAACA+TGG 0.587275 7.4:-24318151 None:intergenic
GTATCTGAATCAGTGTAAAG+AGG 0.596380 7.4:-24318197 None:intergenic
TTTGTCATGCCAAGAAAGCA+CGG 0.601118 7.4:+24318662 MS.gene033616:CDS
GAAGAATATGGAAAGTAGGG+AGG 0.612426 7.4:-24318164 None:intergenic
AGAGAGGACTATTTGCAAGA+AGG 0.626124 7.4:+24318438 MS.gene033616:CDS
ATTGATTGTTATGTAGCTGG+TGG 0.627071 7.4:-24318358 None:intergenic
ATTACTTTCCCTTCGCCTAG+CGG 0.633096 7.4:+24318561 MS.gene033616:CDS
AATTGATATTCAACAAAGGG+TGG 0.651245 7.4:-24318326 None:intergenic
GGGGATCGACCAAAAGAGTG+TGG 0.667307 7.4:+24318303 MS.gene033616:CDS
TAAACCATAAACAAAGAGGT+CGG 0.670477 7.4:-24318499 None:intergenic
GAAGGGAAAGTAATTGAAGG+TGG 0.702291 7.4:-24318552 None:intergenic
TACACTTTAGACATACCTGG+AGG 0.705855 7.4:-24318909 None:intergenic
TGTTATGAGAATTACCAGAG+AGG 0.771694 7.4:+24318422 MS.gene033616:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCTCATAACATGTAAATT+AGG - chr7.4:24318414-24318433 None:intergenic 20.0%
!! TTCTCATAACATGTAAATTA+GGG - chr7.4:24318413-24318432 None:intergenic 20.0%
! AATTGAAGAATATGGAAAGT+AGG - chr7.4:24318171-24318190 None:intergenic 25.0%
! ATTGAAGAATATGGAAAGTA+GGG - chr7.4:24318170-24318189 None:intergenic 25.0%
! CTGATGAATATGTTTATACT+TGG + chr7.4:24318613-24318632 MS.gene033616:CDS 25.0%
! GAATATGTTTATACTTGGTT+TGG + chr7.4:24318618-24318637 MS.gene033616:CDS 25.0%
! GGATTATGAATTGAAGAATA+TGG - chr7.4:24318179-24318198 None:intergenic 25.0%
! TGCAATTGATATTCAACAAA+GGG - chr7.4:24318332-24318351 None:intergenic 25.0%
! TGTGAGAATCTATTTAATTG+TGG + chr7.4:24318246-24318265 MS.gene033616:CDS 25.0%
! TGTGGAACTATAAAACAAAT+TGG + chr7.4:24318264-24318283 MS.gene033616:CDS 25.0%
! TGTTTAAACCATAAACAAAG+AGG - chr7.4:24318506-24318525 None:intergenic 25.0%
AATCAATGAAGTCAAGTATC+TGG + chr7.4:24318374-24318393 MS.gene033616:CDS 30.0%
AATTGATATTCAACAAAGGG+TGG - chr7.4:24318329-24318348 None:intergenic 30.0%
GTGCAATTGATATTCAACAA+AGG - chr7.4:24318333-24318352 None:intergenic 30.0%
TAAACCATAAACAAAGAGGT+CGG - chr7.4:24318502-24318521 None:intergenic 30.0%
! AACAATCTCACACTCTTTTA+TGG + chr7.4:24318525-24318544 MS.gene033616:CDS 30.0%
! TTCATTGATTGTTATGTAGC+TGG - chr7.4:24318364-24318383 None:intergenic 30.0%
!! ACTTGGTTTGGTTCTAATAT+TGG + chr7.4:24318630-24318649 MS.gene033616:CDS 30.0%
!! CACTTTTTATGTCCTTCAAA+TGG + chr7.4:24318585-24318604 MS.gene033616:CDS 30.0%
!!! AACAAATTGGTTTTCCTTTC+TGG + chr7.4:24318277-24318296 MS.gene033616:CDS 30.0%
!!! ACAAATTGGTTTTCCTTTCT+GGG + chr7.4:24318278-24318297 MS.gene033616:CDS 30.0%
AGTAGGGAGGATATTAAACA+TGG - chr7.4:24318154-24318173 None:intergenic 35.0%
ATTGATTGTTATGTAGCTGG+TGG - chr7.4:24318361-24318380 None:intergenic 35.0%
CATAACATGTAAATTAGGGC+TGG - chr7.4:24318409-24318428 None:intergenic 35.0%
GAGCTTCATTAGAACAAGAT+TGG - chr7.4:24318869-24318888 None:intergenic 35.0%
GTATCTGAATCAGTGTAAAG+AGG - chr7.4:24318200-24318219 None:intergenic 35.0%
TAGATTCTCACAACCTTCAT+CGG - chr7.4:24318238-24318257 None:intergenic 35.0%
TCATCAGAAGAACCATTTGA+AGG - chr7.4:24318600-24318619 None:intergenic 35.0%
TGTTATGAGAATTACCAGAG+AGG + chr7.4:24318422-24318441 MS.gene033616:CDS 35.0%
!! CAAAAATCAGGTGGATTTTG+TGG + chr7.4:24318801-24318820 MS.gene033616:CDS 35.0%
!! TGAAGTCAAGTATCTGGTTT+TGG + chr7.4:24318380-24318399 MS.gene033616:CDS 35.0%
!! TGTGAGAAGTGTCAAAAATC+AGG + chr7.4:24318789-24318808 MS.gene033616:CDS 35.0%
!!! CAAATTGGTTTTCCTTTCTG+GGG + chr7.4:24318279-24318298 MS.gene033616:CDS 35.0%
AGAGAGGACTATTTGCAAGA+AGG + chr7.4:24318438-24318457 MS.gene033616:CDS 40.0%
GAAGAATATGGAAAGTAGGG+AGG - chr7.4:24318167-24318186 None:intergenic 40.0%
GAAGGGAAAGTAATTGAAGG+TGG - chr7.4:24318555-24318574 None:intergenic 40.0%
GTACAAAGTGCAATCAGAGA+TGG + chr7.4:24318732-24318751 MS.gene033616:CDS 40.0%
TACACTTTAGACATACCTGG+AGG - chr7.4:24318912-24318931 None:intergenic 40.0%
TCATATTTGAGCTCCGATGA+AGG + chr7.4:24318222-24318241 MS.gene033616:CDS 40.0%
TCATCGGAGCTCAAATATGA+AGG - chr7.4:24318222-24318241 None:intergenic 40.0%
TTCTTGCAAATAGTCCTCTC+TGG - chr7.4:24318439-24318458 None:intergenic 40.0%
TTTGTCATGCCAAGAAAGCA+CGG + chr7.4:24318662-24318681 MS.gene033616:CDS 40.0%
! GTGGATTTTGTGGCTATAAC+TGG + chr7.4:24318811-24318830 MS.gene033616:CDS 40.0%
! TGCAATCAGAGATGGATTTG+TGG + chr7.4:24318740-24318759 MS.gene033616:CDS 40.0%
!! GAGAAGTGTCAAAAATCAGG+TGG + chr7.4:24318792-24318811 MS.gene033616:CDS 40.0%
!!! AGTCAAGTATCTGGTTTTGG+AGG + chr7.4:24318383-24318402 MS.gene033616:CDS 40.0%
AAGCAAACAACCTGCTACTG+TGG + chr7.4:24318840-24318859 MS.gene033616:CDS 45.0%
ACTTTAGACATACCTGGAGG+TGG - chr7.4:24318909-24318928 None:intergenic 45.0%
ATTACTTTCCCTTCGCCTAG+CGG + chr7.4:24318561-24318580 MS.gene033616:CDS 45.0%
GGCGAAGGGAAAGTAATTGA+AGG - chr7.4:24318558-24318577 None:intergenic 45.0%
TTTGTGGTGAGATGGATAGC+TGG + chr7.4:24318756-24318775 MS.gene033616:CDS 45.0%
! GACACCGACCTCTTTGTTTA+TGG + chr7.4:24318495-24318514 MS.gene033616:CDS 45.0%
! GAGATGGATTTGTGGTGAGA+TGG + chr7.4:24318748-24318767 MS.gene033616:CDS 45.0%
!! GGACATAAAAAGTGTCCGCT+AGG - chr7.4:24318579-24318598 None:intergenic 45.0%
!! TTGGTTTTCCTTTCTGGGGA+GGG + chr7.4:24318283-24318302 MS.gene033616:CDS 45.0%
!!! ATTGGTTTTCCTTTCTGGGG+AGG + chr7.4:24318282-24318301 MS.gene033616:CDS 45.0%
CCAGAACAACCGTGCTTTCT+TGG - chr7.4:24318674-24318693 None:intergenic 50.0%
! AAAGGGTGGCCACACTCTTT+TGG - chr7.4:24318315-24318334 None:intergenic 50.0%
! CCAAGAAAGCACGGTTGTTC+TGG + chr7.4:24318671-24318690 MS.gene033616:CDS 50.0%
!! AAAAAGTGTCCGCTAGGCGA+AGG - chr7.4:24318573-24318592 None:intergenic 50.0%
!! AAAAGTGTCCGCTAGGCGAA+GGG - chr7.4:24318572-24318591 None:intergenic 50.0%
!! AGATTGGTCTCCACAGTAGC+AGG - chr7.4:24318853-24318872 None:intergenic 50.0%
!! TGGTTTTCCTTTCTGGGGAG+GGG + chr7.4:24318284-24318303 MS.gene033616:CDS 50.0%
GGGGATCGACCAAAAGAGTG+TGG + chr7.4:24318303-24318322 MS.gene033616:CDS 55.0%
GGTGGAGATTGCTGAGACTG+TGG - chr7.4:24318891-24318910 None:intergenic 55.0%
TCTCAGCAATCTCCACCTCC+AGG + chr7.4:24318894-24318913 MS.gene033616:CDS 55.0%
GGTCGATCCCCTCCCCAGAA+AGG - chr7.4:24318294-24318313 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 24318144 24318932 24318144 ID=MS.gene033616
chr7.4 mRNA 24318144 24318932 24318144 ID=MS.gene033616.t1;Parent=MS.gene033616
chr7.4 exon 24318144 24318932 24318144 ID=MS.gene033616.t1.exon1;Parent=MS.gene033616.t1
chr7.4 CDS 24318144 24318932 24318144 ID=cds.MS.gene033616.t1;Parent=MS.gene033616.t1
Gene Sequence

>MS.gene033616

ATGGCTTCCATGTTTAATATCCTCCCTACTTTCCATATTCTTCAATTCATAATCCTCTTTACACTGATTCAGATACCTTCATATTTGAGCTCCGATGAAGGTTGTGAGAATCTATTTAATTGTGGAACTATAAAACAAATTGGTTTTCCTTTCTGGGGAGGGGATCGACCAAAAGAGTGTGGCCACCCTTTGTTGAATATCAATTGCACAAACACCACCAGCTACATAACAATCAATGAAGTCAAGTATCTGGTTTTGGAGGCCAGCCCTAATTTACATGTTATGAGAATTACCAGAGAGGACTATTTGCAAGAAGGACTATGTTCATCAAAACATTTCAACACAACATTAGACACCGACCTCTTTGTTTATGGTTTAAACAACAATCTCACACTCTTTTATGGTTGTCCACCTTCAATTACTTTCCCTTCGCCTAGCGGACACTTTTTATGTCCTTCAAATGGTTCTTCTGATGAATATGTTTATACTTGGTTTGGTTCTAATATTGGTTCTCAAGCTTTGTCATGCCAAGAAAGCACGGTTGTTCTGGTCACTCAATCATCGATTGATGTTAGTGATTTGACTAAAGTACAAAGTGCAATCAGAGATGGATTTGTGGTGAGATGGATAGCTGGTATTGAAGATTGTGAGAAGTGTCAAAAATCAGGTGGATTTTGTGGCTATAACTGGACTTCAAAGCAAACAACCTGCTACTGTGGAGACCAATCTTGTTCTAATGAAGCTCCACAGTCTCAGCAATCTCCACCTCCAGGTATGTCTAAAGTGTAA

Protein sequence

>MS.gene033616.t1

MASMFNILPTFHILQFIILFTLIQIPSYLSSDEGCENLFNCGTIKQIGFPFWGGDRPKECGHPLLNINCTNTTSYITINEVKYLVLEASPNLHVMRITREDYLQEGLCSSKHFNTTLDTDLFVYGLNNNLTLFYGCPPSITFPSPSGHFLCPSNGSSDEYVYTWFGSNIGSQALSCQESTVVLVTQSSIDVSDLTKVQSAIRDGFVVRWIAGIEDCEKCQKSGGFCGYNWTSKQTTCYCGDQSCSNEAPQSQQSPPPGMSKV