Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033658.t1 | XP_003624429.1 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 8.80E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033658.t1 | B9RH17 | 59.4 | 69 | 28 | 0 | 115 | 183 | 119 | 187 | 2.4e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033658.t1 | G7KW47 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 6.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene033658 | MS.gene025030 | PPI |
| MS.gene033658 | MS.gene63947 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033658.t1 | MTR_7g083120 | 96.774 | 186 | 6 | 0 | 1 | 186 | 1 | 186 | 1.45e-133 | 371 |
| MS.gene033658.t1 | MTR_7g083120 | 95.726 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 3.03e-76 | 227 |
| MS.gene033658.t1 | MTR_4g118810 | 29.655 | 145 | 88 | 3 | 33 | 166 | 36 | 177 | 5.29e-16 | 72.8 |
| MS.gene033658.t1 | MTR_0004s0210 | 29.927 | 137 | 88 | 1 | 33 | 169 | 11 | 139 | 1.59e-15 | 70.9 |
| MS.gene033658.t1 | MTR_4g081880 | 32.099 | 162 | 105 | 3 | 11 | 169 | 10 | 169 | 1.85e-14 | 68.6 |
| MS.gene033658.t1 | MTR_7g108130 | 30.496 | 141 | 92 | 3 | 33 | 169 | 32 | 170 | 6.94e-14 | 67.0 |
| MS.gene033658.t1 | MTR_4g118800 | 36.000 | 125 | 79 | 1 | 33 | 157 | 31 | 154 | 9.21e-13 | 63.9 |
| MS.gene033658.t1 | MTR_2g020060 | 27.778 | 162 | 104 | 2 | 33 | 184 | 26 | 184 | 2.74e-12 | 62.8 |
| MS.gene033658.t1 | MTR_7g011090 | 30.435 | 161 | 107 | 3 | 11 | 169 | 11 | 168 | 1.23e-11 | 60.8 |
| MS.gene033658.t1 | MTR_5g076480 | 24.242 | 165 | 118 | 3 | 22 | 186 | 29 | 186 | 3.80e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033658.t1 | AT4G25040 | 36.424 | 151 | 92 | 1 | 31 | 181 | 18 | 164 | 3.48e-21 | 85.9 |
| MS.gene033658.t1 | AT4G03540 | 34.959 | 123 | 72 | 1 | 47 | 169 | 26 | 140 | 6.31e-18 | 77.0 |
| MS.gene033658.t1 | AT4G15630 | 26.984 | 189 | 119 | 4 | 6 | 184 | 10 | 189 | 1.12e-15 | 72.0 |
| MS.gene033658.t1 | AT4G15620 | 30.435 | 161 | 100 | 3 | 34 | 184 | 31 | 189 | 3.41e-15 | 70.5 |
| MS.gene033658.t1 | AT1G03700 | 32.143 | 112 | 68 | 1 | 45 | 156 | 24 | 127 | 3.23e-14 | 67.4 |
| MS.gene033658.t1 | AT4G15610 | 28.105 | 153 | 105 | 2 | 10 | 157 | 9 | 161 | 4.20e-12 | 62.4 |
| MS.gene033658.t1 | AT5G15290 | 30.216 | 139 | 90 | 3 | 33 | 167 | 31 | 166 | 7.69e-12 | 61.6 |
| MS.gene033658.t1 | AT3G06390 | 25.455 | 165 | 108 | 5 | 5 | 157 | 9 | 170 | 4.19e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGCCTTCTTTGCCAATT+TGG | 0.186565 | 7.4:+23793728 | MS.gene033658:CDS |
| TTGGCCTTCTTTGCCAATTT+GGG | 0.251825 | 7.4:+23793729 | MS.gene033658:CDS |
| AAAGTTCTGCAAAACCAATT+TGG | 0.258540 | 7.4:+23793686 | MS.gene033658:CDS |
| TAAAATTGTGAGTCCCAAAT+TGG | 0.269455 | 7.4:-23793742 | None:intergenic |
| TTCCATCCTGAAGGAATCTT+TGG | 0.318409 | 7.4:-23792316 | None:intergenic |
| AAGCAAAAGTGAAACCAAAT+TGG | 0.326060 | 7.4:-23793700 | None:intergenic |
| GGATGAGGTTGTTGAAGATT+TGG | 0.339479 | 7.4:-23792340 | None:intergenic |
| ACATCATAGGACTTTCTTCA+TGG | 0.382860 | 7.4:+23792371 | MS.gene033658:CDS |
| CTGTTATGCTCTTCTCTATC+CGG | 0.383937 | 7.4:+23792469 | MS.gene033658:CDS |
| GATGAGGTTGTTGAAGATTT+GGG | 0.392076 | 7.4:-23792339 | None:intergenic |
| TAATTAAGAGGTGAACTTGT+AGG | 0.396788 | 7.4:-23793769 | None:intergenic |
| GTTGGATACGTGGGACAGTA+TGG | 0.412618 | 7.4:+23793621 | MS.gene033658:CDS |
| CTGATGCCAAAGATTCCTTC+AGG | 0.434784 | 7.4:+23792310 | MS.gene033658:CDS |
| TCAAAACATCCTCAGAATCT+TGG | 0.440400 | 7.4:+23792395 | MS.gene033658:CDS |
| GAGAGGAGAAAATAATTAAG+AGG | 0.459921 | 7.4:-23793781 | None:intergenic |
| ATGACGGTGTTGCTGATAGC+TGG | 0.469222 | 7.4:+23793579 | MS.gene033658:CDS |
| ATGGAGAGAAACACGTTGGA+TGG | 0.514689 | 7.4:+23793640 | MS.gene033658:CDS |
| GAGCATAACAGATTGGTTGT+TGG | 0.526003 | 7.4:-23792457 | None:intergenic |
| GAGTCCCAAATTGGCAAAGA+AGG | 0.541836 | 7.4:-23793733 | None:intergenic |
| TAGAGAAGAGCATAACAGAT+TGG | 0.552990 | 7.4:-23792464 | None:intergenic |
| AGATTTGGGTTCCATCCTGA+AGG | 0.555523 | 7.4:-23792325 | None:intergenic |
| AGCCACAGCCGTTGGATACG+TGG | 0.562855 | 7.4:+23793611 | MS.gene033658:CDS |
| TGTTCAGCAGCCACAGCCGT+TGG | 0.589627 | 7.4:+23793603 | MS.gene033658:CDS |
| TCCCACGTATCCAACGGCTG+TGG | 0.593429 | 7.4:-23793613 | None:intergenic |
| GCCACAGCCGTTGGATACGT+GGG | 0.599202 | 7.4:+23793612 | MS.gene033658:CDS |
| TGCCAAAGATTCCTTCAGGA+TGG | 0.603471 | 7.4:+23792314 | MS.gene033658:CDS |
| GAACAATGACCAAGATTCTG+AGG | 0.616784 | 7.4:-23792404 | None:intergenic |
| ATACTGTCCCACGTATCCAA+CGG | 0.624247 | 7.4:-23793619 | None:intergenic |
| CAGTATGGAGAGAAACACGT+TGG | 0.708078 | 7.4:+23793636 | MS.gene033658:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATATTATTCTTATAGAA+TGG | + | chr7.4:23793224-23793243 | MS.gene033658:intron | 10.0% |
| !!! | TGATTTTTTTAAATTATTAG+CGG | + | chr7.4:23793377-23793396 | MS.gene033658:intron | 10.0% |
| !! | TCATATGTATAAACAAAAAA+GGG | + | chr7.4:23792563-23792582 | MS.gene033658:intron | 15.0% |
| !! | TTCATATGTATAAACAAAAA+AGG | + | chr7.4:23792562-23792581 | MS.gene033658:intron | 15.0% |
| !! | TTTGTTTATACATATGAAAA+TGG | - | chr7.4:23792560-23792579 | None:intergenic | 15.0% |
| !! | ACTTGACTAAAACATTAAAA+GGG | - | chr7.4:23793110-23793129 | None:intergenic | 20.0% |
| !! | ATACATATGAAAATGGAAAA+TGG | - | chr7.4:23792553-23792572 | None:intergenic | 20.0% |
| !! | TACATATGAAAATGGAAAAT+GGG | - | chr7.4:23792552-23792571 | None:intergenic | 20.0% |
| !! | TTCAGTATAATTATACATGT+CGG | - | chr7.4:23793352-23793371 | None:intergenic | 20.0% |
| !!! | CTATTATTTCTTCCTATTTT+TGG | + | chr7.4:23792736-23792755 | MS.gene033658:CDS | 20.0% |
| !!! | TCTGTATTTATTTTTGTCTT+CGG | + | chr7.4:23792611-23792630 | MS.gene033658:intron | 20.0% |
| ! | AAACAATGACATGTATAGTT+TGG | - | chr7.4:23793166-23793185 | None:intergenic | 25.0% |
| ! | AATAAAAATGAGCTTCAAAC+CGG | - | chr7.4:23792491-23792510 | None:intergenic | 25.0% |
| ! | ATACATGTCATTGTTTATCA+TGG | + | chr7.4:23793170-23793189 | MS.gene033658:intron | 25.0% |
| ! | GACTTGACTAAAACATTAAA+AGG | - | chr7.4:23793111-23793130 | None:intergenic | 25.0% |
| ! | TCTTTCAAATCCACAAAATT+AGG | + | chr7.4:23793487-23793506 | MS.gene033658:intron | 25.0% |
| ! | TTACCAAATCAACCAAAAAT+AGG | - | chr7.4:23792751-23792770 | None:intergenic | 25.0% |
| ! | TTTGAAAGAAAGGTAAATGA+TGG | - | chr7.4:23793476-23793495 | None:intergenic | 25.0% |
| !! | AAATAATAGTCTTTTCGTTG+AGG | - | chr7.4:23792725-23792744 | None:intergenic | 25.0% |
| !! | ATTTTGTGGATTTGAAAGAA+AGG | - | chr7.4:23793486-23793505 | None:intergenic | 25.0% |
| !! | GGAATATGATTGATAATATG+TGG | + | chr7.4:23793191-23793210 | MS.gene033658:intron | 25.0% |
| !! | TGATAATCAATCTTTTGATG+AGG | + | chr7.4:23792690-23792709 | MS.gene033658:CDS | 25.0% |
| !! | TTTACTAGTGTCAATTTTAG+TGG | + | chr7.4:23793022-23793041 | MS.gene033658:intron | 25.0% |
| !! | TTTTAAATTATTAGCGGTGT+CGG | + | chr7.4:23793383-23793402 | MS.gene033658:intron | 25.0% |
| !!! | AAAATTGGTGAATTTTGATC+AGG | + | chr7.4:23793554-23793573 | MS.gene033658:intron | 25.0% |
| !!! | AAGCCAATAACATTTTAACA+TGG | - | chr7.4:23792845-23792864 | None:intergenic | 25.0% |
| !!! | CATGTTTGTTATGAGAAAAT+TGG | + | chr7.4:23793539-23793558 | MS.gene033658:intron | 25.0% |
| !!! | TTACTAGTGTCAATTTTAGT+GGG | + | chr7.4:23793023-23793042 | MS.gene033658:intron | 25.0% |
| !!! | TTCACTTTTGCTTTCATATT+TGG | + | chr7.4:23793710-23793729 | MS.gene033658:CDS | 25.0% |
| AAAAATTGAAGTCGAGACTT+TGG | - | chr7.4:23793005-23793024 | None:intergenic | 30.0% | |
| AAAATTGAAGTCGAGACTTT+GGG | - | chr7.4:23793004-23793023 | None:intergenic | 30.0% | |
| AAAGTTCTGCAAAACCAATT+TGG | + | chr7.4:23793686-23793705 | MS.gene033658:CDS | 30.0% | |
| AAGCAAAAGTGAAACCAAAT+TGG | - | chr7.4:23793703-23793722 | None:intergenic | 30.0% | |
| GGTCCATGTTAAAATGTTAT+TGG | + | chr7.4:23792839-23792858 | MS.gene033658:intron | 30.0% | |
| TAAAATTGTGAGTCCCAAAT+TGG | - | chr7.4:23793745-23793764 | None:intergenic | 30.0% | |
| !! | GAATTTTGATCAGGTAATGA+CGG | + | chr7.4:23793563-23793582 | MS.gene033658:intron | 30.0% |
| !!! | AGATTTTTTGTTGCTGCAAA+TGG | + | chr7.4:23792638-23792657 | MS.gene033658:intron | 30.0% |
| !!! | CTTCCTATTTTTGGTTGATT+TGG | + | chr7.4:23792745-23792764 | MS.gene033658:CDS | 30.0% |
| !!! | TTCACAGATGTTTTTTACTC+TGG | + | chr7.4:23793321-23793340 | MS.gene033658:intron | 30.0% |
| ACATCATAGGACTTTCTTCA+TGG | + | chr7.4:23792371-23792390 | MS.gene033658:CDS | 35.0% | |
| CATCCTCACAAAAACATCAT+AGG | + | chr7.4:23792358-23792377 | MS.gene033658:CDS | 35.0% | |
| GATGAGGTTGTTGAAGATTT+GGG | - | chr7.4:23792342-23792361 | None:intergenic | 35.0% | |
| GGCAGTATCTATATTATGTC+TGG | + | chr7.4:23793409-23793428 | MS.gene033658:intron | 35.0% | |
| TATCCGTATTCATGCTTCAT+AGG | + | chr7.4:23793438-23793457 | MS.gene033658:intron | 35.0% | |
| TCAAAACATCCTCAGAATCT+TGG | + | chr7.4:23792395-23792414 | MS.gene033658:CDS | 35.0% | |
| TGACCTATGAAGCATGAATA+CGG | - | chr7.4:23793444-23793463 | None:intergenic | 35.0% | |
| TGTCTGAAAACAGAAATTGC+AGG | + | chr7.4:23792818-23792837 | MS.gene033658:intron | 35.0% | |
| ! | CTTATTCGCTCCTAATTTTG+TGG | - | chr7.4:23793500-23793519 | None:intergenic | 35.0% |
| ! | TAGAGAAGAGCATAACAGAT+TGG | - | chr7.4:23792467-23792486 | None:intergenic | 35.0% |
| ! | TTGGCTTAGACATGTTTTGT+CGG | + | chr7.4:23792858-23792877 | MS.gene033658:intron | 35.0% |
| ! | TTTTGCAGAACTTTTGCACA+TGG | - | chr7.4:23793680-23793699 | None:intergenic | 35.0% |
| !! | AGTCCTATGATGTTTTTGTG+AGG | - | chr7.4:23792364-23792383 | None:intergenic | 35.0% |
| !! | ATGATGTTTTTGTGAGGATG+AGG | - | chr7.4:23792358-23792377 | None:intergenic | 35.0% |
| !! | TGAAAATGGAAAATGGGTTG+TGG | - | chr7.4:23792546-23792565 | None:intergenic | 35.0% |
| !!! | AGTAGTTTTGAGTTAGACCT+TGG | + | chr7.4:23792967-23792986 | MS.gene033658:intron | 35.0% |
| !!! | GTCAATTTTAGTGGGTTAGT+CGG | + | chr7.4:23793031-23793050 | MS.gene033658:intron | 35.0% |
| CTGTTATGCTCTTCTCTATC+CGG | + | chr7.4:23792469-23792488 | MS.gene033658:CDS | 40.0% | |
| GAACAATGACCAAGATTCTG+AGG | - | chr7.4:23792407-23792426 | None:intergenic | 40.0% | |
| GAGTTGTAACCTACTTAGGT+TGG | + | chr7.4:23792936-23792955 | MS.gene033658:intron | 40.0% | |
| GGATGAGGTTGTTGAAGATT+TGG | - | chr7.4:23792343-23792362 | None:intergenic | 40.0% | |
| TCAACCAAACCAACCTAAGT+AGG | - | chr7.4:23792948-23792967 | None:intergenic | 40.0% | |
| TGGTTAATTACCTCAGCACT+AGG | - | chr7.4:23793146-23793165 | None:intergenic | 40.0% | |
| TTCCATCCTGAAGGAATCTT+TGG | - | chr7.4:23792319-23792338 | None:intergenic | 40.0% | |
| ! | GAGCATAACAGATTGGTTGT+TGG | - | chr7.4:23792460-23792479 | None:intergenic | 40.0% |
| ! | TTGGCCTTCTTTGCCAATTT+GGG | + | chr7.4:23793729-23793748 | MS.gene033658:CDS | 40.0% |
| ! | TTTGGCCTTCTTTGCCAATT+TGG | + | chr7.4:23793728-23793747 | MS.gene033658:CDS | 40.0% |
| ! | TTTTGTTGCTGCAAATGGTG+TGG | + | chr7.4:23792643-23792662 | MS.gene033658:CDS | 40.0% |
| !! | GTAACCTACTTAGGTTGGTT+TGG | + | chr7.4:23792941-23792960 | MS.gene033658:intron | 40.0% |
| ATACTGTCCCACGTATCCAA+CGG | - | chr7.4:23793622-23793641 | None:intergenic | 45.0% | |
| ATGGAGAGAAACACGTTGGA+TGG | + | chr7.4:23793640-23793659 | MS.gene033658:CDS | 45.0% | |
| ATTGAAGTCGAGACTTTGGG+AGG | - | chr7.4:23793001-23793020 | None:intergenic | 45.0% | |
| CAGTATGGAGAGAAACACGT+TGG | + | chr7.4:23793636-23793655 | MS.gene033658:CDS | 45.0% | |
| CGCAGAGTTGTAACCTACTT+AGG | + | chr7.4:23792932-23792951 | MS.gene033658:intron | 45.0% | |
| CTGATGCCAAAGATTCCTTC+AGG | + | chr7.4:23792310-23792329 | MS.gene033658:CDS | 45.0% | |
| GAGTCCCAAATTGGCAAAGA+AGG | - | chr7.4:23793736-23793755 | None:intergenic | 45.0% | |
| TGCCAAAGATTCCTTCAGGA+TGG | + | chr7.4:23792314-23792333 | MS.gene033658:CDS | 45.0% | |
| TTACCTCAGCACTAGGCAAA+CGG | - | chr7.4:23793139-23793158 | None:intergenic | 45.0% | |
| ! | AGATTTGGGTTCCATCCTGA+AGG | - | chr7.4:23792328-23792347 | None:intergenic | 45.0% |
| ! | TTTGGGAGGAGTATACTCCA+AGG | - | chr7.4:23792987-23793006 | None:intergenic | 45.0% |
| !! | AATTATTAGCGGTGTCGGTG+TGG | + | chr7.4:23793388-23793407 | MS.gene033658:intron | 45.0% |
| !!! | AATATTTTTTTTATGTAAAT+CGG | + | chr7.4:23792899-23792918 | MS.gene033658:intron | 5.0% |
| GTTGGATACGTGGGACAGTA+TGG | + | chr7.4:23793621-23793640 | MS.gene033658:CDS | 50.0% | |
| !! | ATGACGGTGTTGCTGATAGC+TGG | + | chr7.4:23793579-23793598 | MS.gene033658:CDS | 50.0% |
| TCTCCGTTTGCCTAGTGCTG+AGG | + | chr7.4:23793133-23793152 | MS.gene033658:intron | 55.0% | |
| AGCCACAGCCGTTGGATACG+TGG | + | chr7.4:23793611-23793630 | MS.gene033658:CDS | 60.0% | |
| GCCACAGCCGTTGGATACGT+GGG | + | chr7.4:23793612-23793631 | MS.gene033658:CDS | 60.0% | |
| TCCCACGTATCCAACGGCTG+TGG | - | chr7.4:23793616-23793635 | None:intergenic | 60.0% | |
| TGTTCAGCAGCCACAGCCGT+TGG | + | chr7.4:23793603-23793622 | MS.gene033658:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 23792306 | 23793788 | 23792306 | ID=MS.gene033658 |
| chr7.4 | mRNA | 23792306 | 23793788 | 23792306 | ID=MS.gene033658.t1;Parent=MS.gene033658 |
| chr7.4 | exon | 23792306 | 23792526 | 23792306 | ID=MS.gene033658.t1.exon1;Parent=MS.gene033658.t1 |
| chr7.4 | CDS | 23792306 | 23792526 | 23792306 | ID=cds.MS.gene033658.t1;Parent=MS.gene033658.t1 |
| chr7.4 | exon | 23792640 | 23792766 | 23792640 | ID=MS.gene033658.t1.exon2;Parent=MS.gene033658.t1 |
| chr7.4 | CDS | 23792640 | 23792766 | 23792640 | ID=cds.MS.gene033658.t1;Parent=MS.gene033658.t1 |
| chr7.4 | exon | 23793576 | 23793788 | 23793576 | ID=MS.gene033658.t1.exon3;Parent=MS.gene033658.t1 |
| chr7.4 | CDS | 23793576 | 23793788 | 23793576 | ID=cds.MS.gene033658.t1;Parent=MS.gene033658.t1 |
| Gene Sequence |
| Protein sequence |
