AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034223

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034223.t1 MTR_4g130820 64.793 747 235 11 1 739 1 727 0.0 951
MS.gene034223.t1 MTR_1g028370 49.732 746 326 12 14 744 5 716 0.0 715
MS.gene034223.t1 MTR_3g091080 50.270 740 321 9 14 739 4 710 0.0 713
MS.gene034223.t1 MTR_2g460590 52.199 682 293 6 82 744 24 691 0.0 708
MS.gene034223.t1 MTR_3g109030 52.246 668 295 7 89 739 61 721 0.0 677
MS.gene034223.t1 MTR_2g094860 49.517 725 327 14 20 738 10 701 0.0 677
MS.gene034223.t1 MTR_7g117310 48.916 738 333 10 17 739 19 727 0.0 666
MS.gene034223.t1 MTR_7g117310 48.780 738 334 10 17 739 7 715 0.0 666
MS.gene034223.t1 MTR_4g058730 55.150 602 257 7 69 666 25 617 0.0 665
MS.gene034223.t1 MTR_7g117310 51.740 661 287 7 92 739 20 661 0.0 664
MS.gene034223.t1 MTR_1g064240 51.420 669 293 13 89 742 59 710 0.0 656
MS.gene034223.t1 MTR_7g034605 51.156 649 294 10 100 738 43 678 0.0 655
MS.gene034223.t1 MTR_8g027755 50.617 648 302 7 97 737 81 717 0.0 645
MS.gene034223.t1 MTR_3g088875 47.661 684 317 12 90 743 20 692 0.0 630
MS.gene034223.t1 MTR_6g477760 40.956 586 310 15 101 666 123 692 1.08e-127 396
MS.gene034223.t1 MTR_6g075290 39.626 588 324 12 100 672 192 763 1.13e-120 380
MS.gene034223.t1 MTR_5g007630 38.047 594 324 15 101 678 172 737 4.25e-117 370
MS.gene034223.t1 MTR_6g075440 34.429 700 400 18 16 686 90 759 5.05e-117 370
MS.gene034223.t1 MTR_6g075440 34.543 689 395 18 16 681 90 745 6.79e-117 370
MS.gene034223.t1 MTR_6g075290 39.160 595 319 16 93 672 156 722 3.71e-110 351
MS.gene034223.t1 MTR_8g464570 35.777 573 348 10 103 664 64 627 8.53e-110 348
MS.gene034223.t1 MTR_6g075290 39.437 568 311 13 120 672 12 561 2.43e-109 344
MS.gene034223.t1 MTR_7g012260 36.333 589 354 9 86 666 102 677 5.60e-109 347
MS.gene034223.t1 MTR_2g081110 34.835 577 351 11 99 664 61 623 1.65e-105 337
MS.gene034223.t1 MTR_6g075410 34.932 584 344 14 100 667 97 660 2.28e-96 313
MS.gene034223.t1 MTR_6g075390 35.596 545 312 13 100 627 114 636 2.04e-92 303
MS.gene034223.t1 MTR_6g076060 36.712 444 249 11 232 660 29 455 2.76e-72 244
MS.gene034223.t1 MTR_6g075320 41.176 119 70 0 100 218 148 266 4.94e-24 103
MS.gene034223.t1 MTR_2g094810 44.954 109 60 0 180 288 12 120 5.22e-24 98.2
MS.gene034223.t1 MTR_4g113530 25.366 205 150 2 379 582 239 441 5.01e-17 86.3
MS.gene034223.t1 MTR_3g108320 25.616 203 148 2 380 582 243 442 2.16e-16 83.6
MS.gene034223.t1 MTR_3g108320 25.616 203 148 2 380 582 243 442 2.28e-16 83.6
MS.gene034223.t1 MTR_3g108320 25.616 203 148 2 380 582 243 442 3.43e-16 83.2
MS.gene034223.t1 MTR_2g006870 24.020 204 152 2 379 582 262 462 7.39e-15 79.0
MS.gene034223.t1 MTR_5g077770 24.194 186 138 2 374 559 277 459 1.47e-12 71.6
MS.gene034223.t1 MTR_8g046440 19.792 192 153 1 374 565 29 219 5.70e-11 65.1
MS.gene034223.t1 MTR_6g075460 41.250 80 45 2 599 676 45 124 7.36e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034223.t1 AT1G19780 68.519 756 215 10 6 744 2 751 0.0 1024
MS.gene034223.t1 AT1G15990 68.133 750 209 14 6 739 2 737 0.0 999
MS.gene034223.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene034223.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene034223.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene034223.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene034223.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene034223.t1 AT4G30560 63.431 752 239 9 1 739 1 729 0.0 949
MS.gene034223.t1 AT4G30560 63.431 752 239 9 1 739 1 729 0.0 949
MS.gene034223.t1 AT5G57940 64.441 734 238 8 11 739 1 716 0.0 943
MS.gene034223.t1 AT5G57940 64.441 734 238 8 11 739 1 716 0.0 943
MS.gene034223.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene034223.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene034223.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene034223.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene034223.t1 AT5G57940 63.896 734 235 8 11 739 1 709 0.0 930
MS.gene034223.t1 AT2G23980 63.049 728 246 9 24 739 1 717 0.0 928
MS.gene034223.t1 AT4G30560 63.636 726 231 8 24 739 1 703 0.0 924
MS.gene034223.t1 AT4G30560 63.636 726 231 8 24 739 1 703 0.0 924
MS.gene034223.t1 AT4G30560 63.636 726 231 8 24 739 1 703 0.0 924
MS.gene034223.t1 AT1G19780 70.425 541 145 7 217 744 2 540 0.0 754
MS.gene034223.t1 AT1G01340 53.707 661 279 6 92 739 59 705 0.0 718
MS.gene034223.t1 AT1G01340 53.707 661 279 6 92 739 54 700 0.0 717
MS.gene034223.t1 AT4G01010 51.489 705 293 11 53 741 24 695 0.0 704
MS.gene034223.t1 AT4G01010 51.489 705 293 11 53 741 24 695 0.0 704
MS.gene034223.t1 AT5G53130 49.730 742 326 10 14 739 4 714 0.0 692
MS.gene034223.t1 AT2G28260 50.946 687 305 8 55 739 21 677 0.0 683
MS.gene034223.t1 AT2G24610 52.444 675 287 11 89 739 60 724 0.0 682
MS.gene034223.t1 AT2G24610 52.444 675 287 11 89 739 75 739 0.0 681
MS.gene034223.t1 AT2G28260 52.024 667 289 7 74 739 1 637 0.0 680
MS.gene034223.t1 AT4G30360 51.672 658 299 10 99 743 69 720 0.0 655
MS.gene034223.t1 AT2G46430 47.241 743 327 12 14 737 5 701 0.0 653
MS.gene034223.t1 AT2G46430 47.241 743 327 12 14 737 5 701 0.0 653
MS.gene034223.t1 AT5G14870 49.552 670 303 9 98 738 37 700 0.0 647
MS.gene034223.t1 AT3G48010 47.640 678 303 12 98 737 41 704 0.0 623
MS.gene034223.t1 AT2G46440 43.339 623 309 13 99 706 28 621 3.96e-162 483
MS.gene034223.t1 AT2G46450 38.473 655 342 16 99 735 28 639 1.38e-148 449
MS.gene034223.t1 AT2G46450 38.473 655 342 16 99 735 28 639 1.38e-148 449
MS.gene034223.t1 AT2G46450 37.557 655 325 17 99 735 15 603 1.39e-140 427
MS.gene034223.t1 AT2G46450 37.557 655 325 17 99 735 28 616 1.87e-140 427
MS.gene034223.t1 AT2G46440 47.097 465 208 10 256 706 3 443 3.37e-130 394
MS.gene034223.t1 AT3G17690 37.563 591 338 14 90 667 147 719 4.21e-119 375
MS.gene034223.t1 AT3G17700 37.544 570 328 10 101 658 192 745 3.76e-115 365
MS.gene034223.t1 AT5G15410 36.678 578 347 9 94 658 106 677 2.80e-108 346
MS.gene034223.t1 AT5G15410 36.678 578 347 9 94 658 105 676 3.00e-108 346
MS.gene034223.t1 AT5G54250 34.549 576 361 8 100 664 78 648 1.42e-107 343
MS.gene034223.t1 AT5G54250 34.549 576 361 8 100 664 78 648 1.42e-107 343
MS.gene034223.t1 AT5G54250 34.549 576 361 8 100 664 78 648 1.42e-107 343
MS.gene034223.t1 AT3G17690 37.897 504 291 10 90 589 147 632 9.99e-106 337
MS.gene034223.t1 AT3G17700 37.576 495 292 7 101 594 192 670 5.03e-103 331
MS.gene034223.t1 AT5G54250 33.854 576 360 9 100 664 78 643 4.07e-102 329
MS.gene034223.t1 AT3G17690 35.433 381 231 8 90 466 147 516 2.81e-66 228
MS.gene034223.t1 AT4G22200 24.020 204 152 2 379 582 263 463 2.28e-16 83.6
MS.gene034223.t1 AT4G22200 24.020 204 152 2 379 582 263 463 5.30e-16 82.8
MS.gene034223.t1 AT5G37500 21.702 470 273 13 99 561 55 436 8.09e-15 79.0
MS.gene034223.t1 AT5G37500 21.489 470 274 13 99 561 55 436 1.01e-14 79.0
MS.gene034223.t1 AT5G37500 20.726 468 280 12 99 561 55 436 1.32e-14 78.2
MS.gene034223.t1 AT5G37500 20.833 408 252 9 155 561 45 382 3.12e-14 77.0
MS.gene034223.t1 AT2G26650 23.902 205 153 2 379 582 239 441 3.87e-14 77.0
MS.gene034223.t1 AT2G26650 23.902 205 153 2 379 582 134 336 5.29e-14 76.3
MS.gene034223.t1 AT5G46240 22.727 198 152 1 379 576 246 442 5.60e-14 76.3
MS.gene034223.t1 AT3G02850 20.902 488 293 14 83 563 56 457 1.04e-13 75.5
MS.gene034223.t1 AT3G02850 20.902 488 293 14 83 563 56 457 1.26e-13 75.1
MS.gene034223.t1 AT3G02850 20.697 488 294 14 83 563 56 457 1.58e-13 74.7
MS.gene034223.t1 AT4G32650 23.153 203 153 2 380 582 276 475 1.58e-12 71.2
MS.gene034223.t1 AT4G32650 23.153 203 153 2 380 582 276 475 2.13e-12 71.2
MS.gene034223.t1 AT4G32650 23.153 203 153 2 380 582 276 475 2.27e-12 70.9

Find 148 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4.1:+88182309 MS.gene034223:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4.1:-88183064 None:intergenic
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4.1:+88181853 MS.gene034223:CDS
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4.1:+88183367 MS.gene034223:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4.1:+88180736 MS.gene034223:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4.1:-88181832 None:intergenic
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4.1:+88183342 MS.gene034223:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4.1:+88183260 MS.gene034223:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4.1:-88180475 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4.1:-88182645 None:intergenic
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4.1:+88180390 MS.gene034223:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4.1:-88180635 None:intergenic
ATGATGTAGGTTGGATGATT+TGG 0.281948 4.1:+88180255 MS.gene034223:intron
CAGAGAAGACTTACATAGTT+AGG 0.283571 4.1:+88182891 MS.gene034223:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4.1:+88182611 MS.gene034223:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4.1:-88182311 None:intergenic
GATATTTCATTATTGACTTC+TGG 0.297261 4.1:+88180812 MS.gene034223:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4.1:+88183343 MS.gene034223:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4.1:-88181817 None:intergenic
CATAGACAGTAACAAAATTT+GGG 0.315147 4.1:-88181831 None:intergenic
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4.1:+88182337 MS.gene034223:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4.1:-88180714 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4.1:+88182310 MS.gene034223:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4.1:-88183480 None:intergenic
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4.1:+88180979 MS.gene034223:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4.1:-88183481 None:intergenic
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4.1:-88180295 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4.1:+88180415 MS.gene034223:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4.1:+88182473 MS.gene034223:CDS
GCTAGATGGCATTGACGAAT+TGG 0.374592 4.1:-88180281 None:intergenic
AATGGTACGGTTCATACCTT+TGG 0.384471 4.1:+88181051 MS.gene034223:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4.1:+88183395 MS.gene034223:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4.1:-88183496 None:intergenic
ATGAACAACATCTCATCAAC+TGG 0.395916 4.1:-88182923 None:intergenic
AAGATAAGAATCTTCTGTAT+TGG 0.412931 4.1:+88180518 MS.gene034223:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4.1:-88183328 None:intergenic
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4.1:+88183415 MS.gene034223:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4.1:-88183085 None:intergenic
TACCTCACTCATAGCTTTCA+CGG 0.425446 4.1:-88183101 None:intergenic
TACGATTTGTGGTTGAAATA+CGG 0.428941 4.1:-88180604 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4.1:+88180834 MS.gene034223:CDS
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4.1:+88180443 MS.gene034223:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4.1:-88183210 None:intergenic
GTGCCATGTACTATTTGATC+TGG 0.449942 4.1:+88181125 MS.gene034223:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4.1:-88182587 None:intergenic
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4.1:-88182292 None:intergenic
TGGCTAAACAATACGATTTG+TGG 0.457799 4.1:-88180615 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4.1:-88181046 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4.1:+88182536 MS.gene034223:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4.1:+88182544 MS.gene034223:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4.1:+88180763 MS.gene034223:CDS
TACAGAAGATTCTTATCTTG+TGG 0.474072 4.1:-88180514 None:intergenic
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4.1:-88180346 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4.1:-88183454 None:intergenic
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4.1:-88182555 None:intergenic
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4.1:-88182560 None:intergenic
TTCTTCACAATGTTTGACTC+TGG 0.491771 4.1:+88179804 None:intergenic
AGAGAAGACTTACATAGTTA+GGG 0.493349 4.1:+88182892 MS.gene034223:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4.1:-88180781 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4.1:+88181595 MS.gene034223:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4.1:+88181620 MS.gene034223:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4.1:+88182817 MS.gene034223:intron
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4.1:+88183126 MS.gene034223:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4.1:+88181648 MS.gene034223:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4.1:+88182679 MS.gene034223:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4.1:+88180444 MS.gene034223:CDS
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4.1:-88180728 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4.1:+88183218 MS.gene034223:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4.1:-88182470 None:intergenic
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4.1:-88180844 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4.1:+88182493 MS.gene034223:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4.1:+88183209 MS.gene034223:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4.1:+88180970 MS.gene034223:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4.1:+88183039 MS.gene034223:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4.1:+88183336 MS.gene034223:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4.1:+88180742 MS.gene034223:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4.1:+88182250 MS.gene034223:intron
TACAAGTCACAGTATATTGA+AGG 0.537328 4.1:+88179828 MS.gene034223:CDS
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4.1:+88183105 MS.gene034223:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4.1:+88182932 MS.gene034223:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4.1:+88182959 MS.gene034223:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4.1:+88181672 MS.gene034223:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4.1:-88182878 None:intergenic
TAGACACATACATAGTAGAC+AGG 0.544177 4.1:+88183174 MS.gene034223:CDS
ACAGTATATTGAAGGTCAGA+AGG 0.547235 4.1:+88179836 MS.gene034223:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4.1:+88182476 MS.gene034223:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4.1:+88182680 MS.gene034223:CDS
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4.1:-88183487 None:intergenic
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4.1:+88182986 MS.gene034223:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4.1:+88182545 MS.gene034223:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4.1:+88180433 MS.gene034223:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4.1:-88183488 None:intergenic
TTTGACGAACTTTGAGGACC+CGG 0.557037 4.1:-88183361 None:intergenic
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4.1:+88181621 MS.gene034223:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4.1:-88183327 None:intergenic
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4.1:+88182484 MS.gene034223:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4.1:+88183450 MS.gene034223:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4.1:-88180739 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4.1:+88182348 MS.gene034223:CDS
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4.1:+88182971 MS.gene034223:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4.1:+88180945 MS.gene034223:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4.1:+88182251 MS.gene034223:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4.1:+88180289 MS.gene034223:CDS
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4.1:+88183276 MS.gene034223:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4.1:+88182311 MS.gene034223:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4.1:+88180445 MS.gene034223:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4.1:+88183272 MS.gene034223:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4.1:+88183245 MS.gene034223:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4.1:+88183266 MS.gene034223:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4.1:+88183309 MS.gene034223:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4.1:-88182523 None:intergenic
ATATACCAGGGTATGACAGT+TGG 0.606713 4.1:+88181685 MS.gene034223:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4.1:+88180942 MS.gene034223:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4.1:+88182279 MS.gene034223:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4.1:+88182962 MS.gene034223:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4.1:+88182464 MS.gene034223:intron
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4.1:+88183279 MS.gene034223:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4.1:-88182552 None:intergenic
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4.1:+88182511 MS.gene034223:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4.1:-88182287 None:intergenic
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4.1:+88183032 MS.gene034223:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4.1:+88183329 MS.gene034223:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4.1:+88182896 MS.gene034223:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4.1:+88182591 MS.gene034223:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4.1:+88182577 MS.gene034223:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4.1:-88182291 None:intergenic
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4.1:+88180379 MS.gene034223:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4.1:-88182821 None:intergenic
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4.1:+88183330 MS.gene034223:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4.1:-88181128 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4.1:-88181690 None:intergenic
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4.1:+88180290 MS.gene034223:CDS
ATAGACAGTAACAAAATTTG+GGG 0.668163 4.1:-88181830 None:intergenic
GTCAGAAGGAGAAATTTGTG+AGG 0.668583 4.1:+88179850 MS.gene034223:CDS
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4.1:+88181559 MS.gene034223:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4.1:+88182274 MS.gene034223:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4.1:-88180725 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4.1:+88182592 MS.gene034223:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4.1:+88183099 MS.gene034223:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4.1:+88183219 MS.gene034223:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4.1:+88183033 MS.gene034223:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4.1:-88180720 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4.1:+88183310 MS.gene034223:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4.1:-88182466 None:intergenic
TCTATATTCAAGCTGCATGG+AGG 0.707083 4.1:+88183248 MS.gene034223:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4.1:+88180973 MS.gene034223:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4.1:+88181673 MS.gene034223:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4.1:+88182593 MS.gene034223:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTAAAATTCTATAAATTA+TGG - chr4.1:88182098-88182117 None:intergenic 10.0%
!! AAATATATATGTATTAGTGT+CGG + chr4.1:88181976-88181995 MS.gene034223:intron 15.0%
!! ATATTTATAAAGGATGATAT+AGG - chr4.1:88182147-88182166 None:intergenic 15.0%
!!! AACTATTATTTTGTAGATAA+CGG + chr4.1:88181548-88181567 MS.gene034223:intron 15.0%
!! ATGTTTACAGTAAAAACTTT+CGG - chr4.1:88182378-88182397 None:intergenic 20.0%
!! GAATCAAAGCATATTTATAA+AGG - chr4.1:88182157-88182176 None:intergenic 20.0%
!!! ACTATTATTTTGTAGATAAC+GGG + chr4.1:88181549-88181568 MS.gene034223:intron 20.0%
!!! TATATTCCACTTTAAGTTTA+GGG + chr4.1:88180179-88180198 MS.gene034223:intron 20.0%
!!! TATTGGTTAATTTAAGAACA+TGG + chr4.1:88180119-88180138 MS.gene034223:intron 20.0%
!!! TGTGAGGTATTTTTATTTTT+GGG + chr4.1:88179866-88179885 MS.gene034223:intron 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG - chr4.1:88181939-88181958 None:intergenic 20.0%
!!! TTATATTCCACTTTAAGTTT+AGG + chr4.1:88180178-88180197 MS.gene034223:intron 20.0%
!!! TTGTGAGGTATTTTTATTTT+TGG + chr4.1:88179865-88179884 MS.gene034223:intron 20.0%
! AAGATAAGAATCTTCTGTAT+TGG + chr4.1:88180518-88180537 MS.gene034223:CDS 25.0%
! AATTCACATTTCTTGTATTG+TGG + chr4.1:88181648-88181667 MS.gene034223:CDS 25.0%
! AGATGCTTATGTTAACAAAT+TGG - chr4.1:88180215-88180234 None:intergenic 25.0%
! ATAGACAGTAACAAAATTTG+GGG - chr4.1:88181833-88181852 None:intergenic 25.0%
! CAACAAACATTAAGACATAT+TGG - chr4.1:88180930-88180949 None:intergenic 25.0%
! CATAGACAGTAACAAAATTT+GGG - chr4.1:88181834-88181853 None:intergenic 25.0%
! CTTCAAAAAAAAAAACCACT+TGG + chr4.1:88180093-88180112 MS.gene034223:intron 25.0%
! GTATAACATATCAATGTGAT+TGG + chr4.1:88179888-88179907 MS.gene034223:intron 25.0%
! GTGTATGACATATATTATTC+AGG + chr4.1:88182796-88182815 MS.gene034223:intron 25.0%
! TATCATTCACATATTCTCTT+TGG + chr4.1:88181280-88181299 MS.gene034223:intron 25.0%
! TCCATAGTAATAGCTATTAT+GGG + chr4.1:88182310-88182329 MS.gene034223:CDS 25.0%
! TCTTAAGAAATGAACTTACA+TGG - chr4.1:88181165-88181184 None:intergenic 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG - chr4.1:88182766-88182785 None:intergenic 25.0%
! TTCCATAGTAATAGCTATTA+TGG + chr4.1:88182309-88182328 MS.gene034223:CDS 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG + chr4.1:88182680-88182699 MS.gene034223:CDS 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG + chr4.1:88182679-88182698 MS.gene034223:CDS 25.0%
!! TAGAAAAATTGGATGCTTTT+TGG - chr4.1:88181324-88181343 None:intergenic 25.0%
!!! AAAAGCATCCAATTTTTCTA+TGG + chr4.1:88181324-88181343 MS.gene034223:intron 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG - chr4.1:88181397-88181416 None:intergenic 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG - chr4.1:88182648-88182667 None:intergenic 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG - chr4.1:88181357-88181376 None:intergenic 25.0%
!!! GATATTTCATTATTGACTTC+TGG + chr4.1:88180812-88180831 MS.gene034223:CDS 25.0%
!!! GTTTTATAGGTGTTATAAAG+AGG - chr4.1:88181926-88181945 None:intergenic 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG - chr4.1:88181356-88181375 None:intergenic 25.0%
AAAGCATGTAAAGAAGTTGA+GGG + chr4.1:88181621-88181640 MS.gene034223:CDS 30.0%
AAATTTGGGGAAAACTTCTA+TGG - chr4.1:88181820-88181839 None:intergenic 30.0%
AAGCAAAACAGAAGGATATT+TGG - chr4.1:88182231-88182250 None:intergenic 30.0%
AATTAACCAATAGAACCAAG+TGG - chr4.1:88180111-88180130 None:intergenic 30.0%
AGAGAAGACTTACATAGTTA+GGG + chr4.1:88182892-88182911 MS.gene034223:CDS 30.0%
ATACCAGATCAAATAGTACA+TGG - chr4.1:88181131-88181150 None:intergenic 30.0%
ATATTACGAATCTGATTACC+CGG + chr4.1:88183309-88183328 MS.gene034223:CDS 30.0%
ATGGCTAATGTGAAAAACAT+AGG - chr4.1:88182005-88182024 None:intergenic 30.0%
ATTGTAGATTCACTTGATGT+TGG + chr4.1:88182116-88182135 MS.gene034223:intron 30.0%
CCAATTGCATTTATAACACA+AGG - chr4.1:88182441-88182460 None:intergenic 30.0%
CCATAGACAGTAACAAAATT+TGG - chr4.1:88181835-88181854 None:intergenic 30.0%
CCATAGTAATAGCTATTATG+GGG + chr4.1:88182311-88182330 MS.gene034223:CDS 30.0%
CCTTGTGTTATAAATGCAAT+TGG + chr4.1:88182438-88182457 MS.gene034223:intron 30.0%
CTCGATAGATTTGTCATTAA+CGG + chr4.1:88180045-88180064 MS.gene034223:intron 30.0%
GGAAGTTTCCATAGAAAAAT+TGG - chr4.1:88181335-88181354 None:intergenic 30.0%
GTCTTCTCTGTATATAAACT+CGG - chr4.1:88182881-88182900 None:intergenic 30.0%
GTTAGAATAGATTACGCTAT+AGG + chr4.1:88181474-88181493 MS.gene034223:intron 30.0%
TAATATACAGTGGATGATGT+AGG + chr4.1:88180242-88180261 MS.gene034223:intron 30.0%
TACAAGTCACAGTATATTGA+AGG + chr4.1:88179828-88179847 MS.gene034223:CDS 30.0%
TACAGAAGATTCTTATCTTG+TGG - chr4.1:88180517-88180536 None:intergenic 30.0%
TACGATTTGTGGTTGAAATA+CGG - chr4.1:88180607-88180626 None:intergenic 30.0%
TACGGAAGATAGAAAAAGAA+AGG - chr4.1:88180589-88180608 None:intergenic 30.0%
TCTGATCTTCATATCAAAGT+CGG + chr4.1:88182053-88182072 MS.gene034223:intron 30.0%
TCTTTCAGACTAATATACAG+TGG + chr4.1:88180232-88180251 MS.gene034223:intron 30.0%
TTGTAGATATTCTGACATAG+AGG + chr4.1:88181452-88181471 MS.gene034223:intron 30.0%
TTGTAGATTCACTTGATGTT+GGG + chr4.1:88182117-88182136 MS.gene034223:intron 30.0%
! AATATTTTCCGAGAATGGTA+CGG + chr4.1:88181038-88181057 MS.gene034223:CDS 30.0%
! AATTTTGTTACTGTCTATGG+TGG + chr4.1:88181835-88181854 MS.gene034223:CDS 30.0%
! ATTTTGTTACTGTCTATGGT+GGG + chr4.1:88181836-88181855 MS.gene034223:CDS 30.0%
! CCAAATTTTGTTACTGTCTA+TGG + chr4.1:88181832-88181851 MS.gene034223:CDS 30.0%
! CTCCATAAAAGAAAAAGTGT+TGG + chr4.1:88181240-88181259 MS.gene034223:intron 30.0%
! GACTCTATTAATCTGGATTT+TGG + chr4.1:88179960-88179979 MS.gene034223:intron 30.0%
!! AAAAAACCACTTGGTTCTAT+TGG + chr4.1:88180102-88180121 MS.gene034223:intron 30.0%
!! GATAACTCAGCTTTTAACTA+TGG + chr4.1:88181756-88181775 MS.gene034223:CDS 30.0%
!! TATTAATCTGGATTTTGGTC+AGG + chr4.1:88179965-88179984 MS.gene034223:intron 30.0%
!! TCAGATGGACTAATGAATAT+TGG + chr4.1:88180415-88180434 MS.gene034223:CDS 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG + chr4.1:88181103-88181122 MS.gene034223:CDS 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG - chr4.1:88181396-88181415 None:intergenic 30.0%
!!! CACCAACACTTTTTCTTTTA+TGG - chr4.1:88181245-88181264 None:intergenic 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG - chr4.1:88181070-88181089 None:intergenic 30.0%
AAGACTTACATAGTTAGGGA+AGG + chr4.1:88182896-88182915 MS.gene034223:CDS 35.0%
ACAGTATATTGAAGGTCAGA+AGG + chr4.1:88179836-88179855 MS.gene034223:CDS 35.0%
ACTGTGAAAAGCAAAACAGA+AGG - chr4.1:88182239-88182258 None:intergenic 35.0%
AGAATAGATTACGCTATAGG+CGG + chr4.1:88181477-88181496 MS.gene034223:intron 35.0%
AGTTCAAGTAAGCATATACC+AGG + chr4.1:88181672-88181691 MS.gene034223:CDS 35.0%
ATACAACAGTCTATGCTTCT+CGG + chr4.1:88183395-88183414 MS.gene034223:CDS 35.0%
ATGAACAACATCTCATCAAC+TGG - chr4.1:88182926-88182945 None:intergenic 35.0%
ATTGGAAGATCACTCAAAAG+TGG + chr4.1:88180433-88180452 MS.gene034223:CDS 35.0%
CAAAACATATTGAGCATGGA+TGG - chr4.1:88180869-88180888 None:intergenic 35.0%
CAATCTATATTCAAGCTGCA+TGG + chr4.1:88183245-88183264 MS.gene034223:CDS 35.0%
CAGAGAAGACTTACATAGTT+AGG + chr4.1:88182891-88182910 MS.gene034223:CDS 35.0%
CATAAGTGATACAGAAGCAA+TGG - chr4.1:88181209-88181228 None:intergenic 35.0%
CCCCATAATAGCTATTACTA+TGG - chr4.1:88182314-88182333 None:intergenic 35.0%
CCTCAAAGTTCGTCAAAATT+TGG + chr4.1:88183367-88183386 MS.gene034223:CDS 35.0%
GAAACATTGATCATTCGTGT+CGG - chr4.1:88181523-88181542 None:intergenic 35.0%
GAACCAGAGAGTTAAGATAA+CGG - chr4.1:88179927-88179946 None:intergenic 35.0%
GGAAAGAGACTCTATTAATC+TGG + chr4.1:88179953-88179972 MS.gene034223:intron 35.0%
GTTCAAGTAAGCATATACCA+GGG + chr4.1:88181673-88181692 MS.gene034223:CDS 35.0%
TACGCAAAACATATTGAGCA+TGG - chr4.1:88180873-88180892 None:intergenic 35.0%
TAGACACATACATAGTAGAC+AGG + chr4.1:88183174-88183193 MS.gene034223:CDS 35.0%
TATTACGAATCTGATTACCC+GGG + chr4.1:88183310-88183329 MS.gene034223:CDS 35.0%
TCAGAAGTTAAAAAGACAGC+TGG + chr4.1:88181076-88181095 MS.gene034223:CDS 35.0%
TCCATTGTTGCGAATAGAAA+CGG - chr4.1:88183213-88183232 None:intergenic 35.0%
TCTGTATCACTTATGAAGCT+CGG + chr4.1:88181214-88181233 MS.gene034223:intron 35.0%
TGCAATGAGAAATCTCAGAA+AGG - chr4.1:88182730-88182749 None:intergenic 35.0%
TGCATACCCTAAACTTAAAG+TGG - chr4.1:88180188-88180207 None:intergenic 35.0%
TGGCTAAACAATACGATTTG+TGG - chr4.1:88180618-88180637 None:intergenic 35.0%
TGTTATAAAGAGGACTCATG+TGG - chr4.1:88181916-88181935 None:intergenic 35.0%
TTTCCGTTATCTTAACTCTC+TGG + chr4.1:88179921-88179940 MS.gene034223:intron 35.0%
! AATCTATCGAGAGTATATCC+AGG - chr4.1:88180036-88180055 None:intergenic 35.0%
! ATGATGTAGGTTGGATGATT+TGG + chr4.1:88180255-88180274 MS.gene034223:intron 35.0%
! CATGCAATATTTTCCGAGAA+TGG + chr4.1:88181033-88181052 MS.gene034223:CDS 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG + chr4.1:88181620-88181639 MS.gene034223:CDS 35.0%
! GAAGTATCAAGAAGTGTATG+AGG - chr4.1:88181374-88181393 None:intergenic 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG + chr4.1:88183013-88183032 MS.gene034223:CDS 35.0%
! TTTTCACATTAGCCATGTAG+AGG + chr4.1:88182009-88182028 MS.gene034223:intron 35.0%
! TTTTGTTACTGTCTATGGTG+GGG + chr4.1:88181837-88181856 MS.gene034223:CDS 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG - chr4.1:88180638-88180657 None:intergenic 35.0%
!! CCAAATTTTGACGAACTTTG+AGG - chr4.1:88183370-88183389 None:intergenic 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG + chr4.1:88180942-88180961 MS.gene034223:intron 35.0%
!! TATCTTAACTCTCTGGTTCT+CGG + chr4.1:88179928-88179947 MS.gene034223:intron 35.0%
!! TGTGACACTTTAAGATCTTC+TGG - chr4.1:88180478-88180497 None:intergenic 35.0%
!! TTTGCTTTTCACAGTACACT+TGG + chr4.1:88182244-88182263 MS.gene034223:intron 35.0%
!! TTTTTGAAGAGAGAACCAAG+TGG - chr4.1:88180082-88180101 None:intergenic 35.0%
AATGGTACGGTTCATACCTT+TGG + chr4.1:88181051-88181070 MS.gene034223:CDS 40.0%
ACTCAAAAGTGGAGTCACTT+GGG + chr4.1:88180444-88180463 MS.gene034223:CDS 40.0%
ACTTCTTGATACTTCCCGAT+TGG + chr4.1:88181379-88181398 MS.gene034223:intron 40.0%
AGGGGTCGATGAAGAAAATT+TGG + chr4.1:88182611-88182630 MS.gene034223:CDS 40.0%
ATACAGTGGATGATGTAGGT+TGG + chr4.1:88180246-88180265 MS.gene034223:intron 40.0%
ATATACCAGGGTATGACAGT+TGG + chr4.1:88181685-88181704 MS.gene034223:CDS 40.0%
ATGGAAAACAAAACCTCTCC+AGG - chr4.1:88182295-88182314 None:intergenic 40.0%
ATTCGCAACAATGGAGAACA+TGG + chr4.1:88183218-88183237 MS.gene034223:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG - chr4.1:88180742-88180761 None:intergenic 40.0%
GAAGATCAGACACACGATAT+GGG - chr4.1:88182043-88182062 None:intergenic 40.0%
GACCTACCTTCAATCAATGT+CGG + chr4.1:88182464-88182483 MS.gene034223:intron 40.0%
GAGATGTTGTTCATCATTCG+TGG + chr4.1:88182932-88182951 MS.gene034223:CDS 40.0%
GTCAGAAGGAGAAATTTGTG+AGG + chr4.1:88179850-88179869 MS.gene034223:CDS 40.0%
GTGCCATGTACTATTTGATC+TGG + chr4.1:88181125-88181144 MS.gene034223:CDS 40.0%
TACCTCACTCATAGCTTTCA+CGG - chr4.1:88183104-88183123 None:intergenic 40.0%
TCATCTCGAGTATTTGGAAG+AGG + chr4.1:88180742-88180761 MS.gene034223:CDS 40.0%
TCCGTTTCTATTCGCAACAA+TGG + chr4.1:88183209-88183228 MS.gene034223:CDS 40.0%
TCTATATTCAAGCTGCATGG+AGG + chr4.1:88183248-88183267 MS.gene034223:CDS 40.0%
TCTTCTGATTGGAAACATGC+AGG + chr4.1:88182348-88182367 MS.gene034223:CDS 40.0%
TGAAGATCAGACACACGATA+TGG - chr4.1:88182044-88182063 None:intergenic 40.0%
TGGAAAACAAAACCTCTCCA+GGG - chr4.1:88182294-88182313 None:intergenic 40.0%
TGGAGGAAATGAGGATCAAA+AGG + chr4.1:88182493-88182512 MS.gene034223:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG + chr4.1:88183033-88183052 MS.gene034223:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG + chr4.1:88180945-88180964 MS.gene034223:intron 40.0%
TTCGCAACAATGGAGAACAT+GGG + chr4.1:88183219-88183238 MS.gene034223:CDS 40.0%
TTTCACAGTACACTTGGTCA+GGG + chr4.1:88182250-88182269 MS.gene034223:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG - chr4.1:88182469-88182488 None:intergenic 40.0%
! AATTTTCTTCATCGACCCCT+CGG - chr4.1:88182611-88182630 None:intergenic 40.0%
! ATCGAGAGTATATCCAGGTT+CGG - chr4.1:88180031-88180050 None:intergenic 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG + chr4.1:88180390-88180409 MS.gene034223:CDS 40.0%
! TCGTCGAAATCATTTCGAGT+CGG + chr4.1:88180379-88180398 MS.gene034223:CDS 40.0%
! TGTTTGCCTTTTCCTATTCC+CGG + chr4.1:88180340-88180359 MS.gene034223:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG - chr4.1:88183088-88183107 None:intergenic 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG + chr4.1:88180391-88180410 MS.gene034223:CDS 40.0%
! TTTTCACAGTACACTTGGTC+AGG + chr4.1:88182249-88182268 MS.gene034223:intron 40.0%
!! ATCTTAACTCTCTGGTTCTC+GGG + chr4.1:88179929-88179948 MS.gene034223:intron 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG + chr4.1:88182959-88182978 MS.gene034223:CDS 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG + chr4.1:88182997-88183016 MS.gene034223:CDS 40.0%
!! TCATTGAGAGCACTGAAATC+AGG - chr4.1:88183499-88183518 None:intergenic 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG + chr4.1:88183001-88183020 MS.gene034223:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG - chr4.1:88180366-88180385 None:intergenic 40.0%
AAACAAAACCTCTCCAGGGT+AGG - chr4.1:88182290-88182309 None:intergenic 45.0%
AACCGTGAAAGCTATGAGTG+AGG + chr4.1:88183099-88183118 MS.gene034223:CDS 45.0%
ACGGAAGTTCATCAACCACT+TGG + chr4.1:88180064-88180083 MS.gene034223:intron 45.0%
AGAAGTGGCTTCTTCAACAG+AGG + chr4.1:88182986-88183005 MS.gene034223:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG + chr4.1:88183039-88183058 MS.gene034223:CDS 45.0%
AGTACCAAGTTCAAGAGAGC+TGG + chr4.1:88183450-88183469 MS.gene034223:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG + chr4.1:88180443-88180462 MS.gene034223:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG + chr4.1:88180445-88180464 MS.gene034223:CDS 45.0%
CTTGCAGAATCATCACTTCC+CGG - chr4.1:88183331-88183350 None:intergenic 45.0%
GAAAGCTATGAGTGAGGTAG+AGG + chr4.1:88183105-88183124 MS.gene034223:CDS 45.0%
GCACCTTCATCTCGAGTATT+TGG + chr4.1:88180736-88180755 MS.gene034223:CDS 45.0%
GGAGTGAAAAAGGGATCAGA+TGG + chr4.1:88180400-88180419 MS.gene034223:CDS 45.0%
GGGAAGTGATGATTCTGCAA+GGG + chr4.1:88183330-88183349 MS.gene034223:CDS 45.0%
GGGTGTTCAACCACTTGTAT+TGG - chr4.1:88182590-88182609 None:intergenic 45.0%
GGTATGAACCGTACCATTCT+CGG - chr4.1:88181049-88181068 None:intergenic 45.0%
GGTGAACTTGTCATAGATCC+TGG + chr4.1:88180763-88180782 MS.gene034223:CDS 45.0%
GGTGGGGACTTCAGAATTTA+AGG + chr4.1:88181853-88181872 MS.gene034223:CDS 45.0%
GGTTCCTCTCTTTGCTAACA+TGG + chr4.1:88182817-88182836 MS.gene034223:intron 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG + chr4.1:88182337-88182356 MS.gene034223:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG + chr4.1:88183032-88183051 MS.gene034223:CDS 45.0%
TAGAACGTAACGACACATGC+TGG + chr4.1:88181595-88181614 MS.gene034223:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG - chr4.1:88180784-88180803 None:intergenic 45.0%
TCGTCCATGTTAGCAAAGAG+AGG - chr4.1:88182824-88182843 None:intergenic 45.0%
TCTTTATAGCGCAAGACGCA+TGG + chr4.1:88180970-88180989 MS.gene034223:CDS 45.0%
TGGATGGTATCTCAAACCTG+TGG - chr4.1:88180853-88180872 None:intergenic 45.0%
TTCACAGTACACTTGGTCAG+GGG + chr4.1:88182251-88182270 MS.gene034223:intron 45.0%
TTCACCAGCTCTCTTGAACT+TGG - chr4.1:88183457-88183476 None:intergenic 45.0%
TTCATCCCACTTGAGCTAGA+TGG - chr4.1:88180298-88180317 None:intergenic 45.0%
TTGCAGAATCATCACTTCCC+GGG - chr4.1:88183330-88183349 None:intergenic 45.0%
TTTCTCCAACTGTCATACCC+TGG - chr4.1:88181693-88181712 None:intergenic 45.0%
! AGATGAAGGTGCAATGAAGG+CGG - chr4.1:88180728-88180747 None:intergenic 45.0%
! AGCACTGAAATCAGGTTCTG+AGG - chr4.1:88183491-88183510 None:intergenic 45.0%
! ATTTTCTTCATCGACCCCTC+GGG - chr4.1:88182610-88182629 None:intergenic 45.0%
! CAATGCCATCTAGCTCAAGT+GGG + chr4.1:88180290-88180309 MS.gene034223:CDS 45.0%
! GACGAACCGACATTGATTGA+AGG - chr4.1:88182473-88182492 None:intergenic 45.0%
! GCACTGAAATCAGGTTCTGA+GGG - chr4.1:88183490-88183509 None:intergenic 45.0%
! TCAATGCCATCTAGCTCAAG+TGG + chr4.1:88180289-88180308 MS.gene034223:CDS 45.0%
! TCGAGATGAAGGTGCAATGA+AGG - chr4.1:88180731-88180750 None:intergenic 45.0%
! TTTGACGAACTTTGAGGACC+CGG - chr4.1:88183364-88183383 None:intergenic 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG - chr4.1:88183484-88183503 None:intergenic 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG - chr4.1:88183483-88183502 None:intergenic 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG + chr4.1:88182971-88182990 MS.gene034223:CDS 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG + chr4.1:88183260-88183279 MS.gene034223:CDS 45.0%
!! GAGAGTGTTACTACAGATGG+TGG + chr4.1:88182962-88182981 MS.gene034223:CDS 45.0%
!! GCTAGATGGCATTGACGAAT+TGG - chr4.1:88180284-88180303 None:intergenic 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG + chr4.1:88183126-88183145 MS.gene034223:CDS 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG + chr4.1:88182473-88182492 MS.gene034223:CDS 45.0%
!! TCTTAACTCTCTGGTTCTCG+GGG + chr4.1:88179930-88179949 MS.gene034223:intron 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG + chr4.1:88181559-88181578 MS.gene034223:intron 45.0%
!!! AATTGAATATAAATAAATTT+TGG - chr4.1:88181408-88181427 None:intergenic 5.0%
ACAAGTGGTTGAACACCCGA+GGG + chr4.1:88182592-88182611 MS.gene034223:CDS 50.0%
AGCGTCTGACTCTTTCCCTT+AGG - chr4.1:88182563-88182582 None:intergenic 50.0%
ATCCAGGTTCGGATCTTGAC+TGG - chr4.1:88180020-88180039 None:intergenic 50.0%
CGGGAAGTGATGATTCTGCA+AGG + chr4.1:88183329-88183348 MS.gene034223:CDS 50.0%
CTGACTCTTTCCCTTAGGTC+CGG - chr4.1:88182558-88182577 None:intergenic 50.0%
CTTCTGACTAGTACCTACCC+TGG + chr4.1:88182274-88182293 MS.gene034223:CDS 50.0%
GACGCTACGACCAATACAAG+TGG + chr4.1:88182577-88182596 MS.gene034223:CDS 50.0%
GTATCTCAAACCTGTGGCAG+TGG - chr4.1:88180847-88180866 None:intergenic 50.0%
TACAAGTGGTTGAACACCCG+AGG + chr4.1:88182591-88182610 MS.gene034223:CDS 50.0%
TTATAGCGCAAGACGCATGG+AGG + chr4.1:88180973-88180992 MS.gene034223:CDS 50.0%
TTCCAGTCAAGATCCGAACC+TGG + chr4.1:88180015-88180034 MS.gene034223:intron 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG + chr4.1:88183272-88183291 MS.gene034223:CDS 50.0%
! TGATGATTCTGCAAGGGCGT+TGG + chr4.1:88183336-88183355 MS.gene034223:CDS 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG + chr4.1:88183276-88183295 MS.gene034223:CDS 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG + chr4.1:88182476-88182495 MS.gene034223:CDS 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG + chr4.1:88183415-88183434 MS.gene034223:CDS 50.0%
!! CTTAACTCTCTGGTTCTCGG+GGG + chr4.1:88179931-88179950 MS.gene034223:intron 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG + chr4.1:88183266-88183285 MS.gene034223:CDS 50.0%
!! TTAACTCTCTGGTTCTCGGG+GGG + chr4.1:88179932-88179951 MS.gene034223:intron 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG - chr4.1:88180362-88180381 None:intergenic 50.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG - chr4.1:88180361-88180380 None:intergenic 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG - chr4.1:88183067-88183086 None:intergenic 50.0%
AAGGTGCAATGAAGGCGGTG+CGG - chr4.1:88180723-88180742 None:intergenic 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG - chr4.1:88182555-88182574 None:intergenic 55.0%
CAAGTGGTTGAACACCCGAG+GGG + chr4.1:88182593-88182612 MS.gene034223:CDS 55.0%
CAATGAAGGCGGTGCGGAAT+TGG - chr4.1:88180717-88180736 None:intergenic 55.0%
CGGTTCGTCTGGAGGAAATG+AGG + chr4.1:88182484-88182503 MS.gene034223:CDS 55.0%
CTTGCTTCCTCCGGACCTAA+GGG + chr4.1:88182545-88182564 MS.gene034223:CDS 55.0%
GACTAGTACCTACCCTGGAG+AGG + chr4.1:88182279-88182298 MS.gene034223:CDS 55.0%
GCATCATCGCTTGCTTCCTC+CGG + chr4.1:88182536-88182555 MS.gene034223:CDS 55.0%
GCGATGATGCATCCACTGCT+CGG - chr4.1:88182526-88182545 None:intergenic 55.0%
TGGGCACATGCACCTCTACA+TGG - chr4.1:88182024-88182043 None:intergenic 55.0%
! CGCAAGACGCATGGAGGTAT+TGG + chr4.1:88180979-88180998 MS.gene034223:CDS 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG + chr4.1:88183342-88183361 MS.gene034223:CDS 55.0%
AAGGCGAAGGCGAAGGAAGG+AGG + chr4.1:88183279-88183298 MS.gene034223:CDS 60.0%
GCTTGCTTCCTCCGGACCTA+AGG + chr4.1:88182544-88182563 MS.gene034223:CDS 60.0%
GTCTGTACTGCCACTGCCAC+AGG + chr4.1:88180834-88180853 MS.gene034223:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG + chr4.1:88182511-88182530 MS.gene034223:CDS 60.0%
! CCTTTTCCTATTCCCGGCCG+CGG + chr4.1:88180346-88180365 MS.gene034223:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG + chr4.1:88180347-88180366 MS.gene034223:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG - chr4.1:88180355-88180374 None:intergenic 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG + chr4.1:88183343-88183362 MS.gene034223:CDS 60.0%
CCGCGGCCGGGAATAGGAAA+AGG - chr4.1:88180349-88180368 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 88179813 88183528 88179813 ID=MS.gene034223
chr4.1 mRNA 88179813 88183528 88179813 ID=MS.gene034223.t1;Parent=MS.gene034223
chr4.1 exon 88179813 88179871 88179813 ID=MS.gene034223.t1.exon1;Parent=MS.gene034223.t1
chr4.1 CDS 88179813 88179871 88179813 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
chr4.1 exon 88180264 88180855 88180264 ID=MS.gene034223.t1.exon2;Parent=MS.gene034223.t1
chr4.1 CDS 88180264 88180855 88180264 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
chr4.1 exon 88180953 88181165 88180953 ID=MS.gene034223.t1.exon3;Parent=MS.gene034223.t1
chr4.1 CDS 88180953 88181165 88180953 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
chr4.1 exon 88181564 88181874 88181564 ID=MS.gene034223.t1.exon4;Parent=MS.gene034223.t1
chr4.1 CDS 88181564 88181874 88181564 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
chr4.1 exon 88182258 88182369 88182258 ID=MS.gene034223.t1.exon5;Parent=MS.gene034223.t1
chr4.1 CDS 88182258 88182369 88182258 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
chr4.1 exon 88182465 88182701 88182465 ID=MS.gene034223.t1.exon6;Parent=MS.gene034223.t1
chr4.1 CDS 88182465 88182701 88182465 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
chr4.1 exon 88182818 88183528 88182818 ID=MS.gene034223.t1.exon7;Parent=MS.gene034223.t1
chr4.1 CDS 88182818 88183528 88182818 ID=cds.MS.gene034223.t1;Parent=MS.gene034223.t1
Gene Sequence

>MS.gene034223

ATGTTTGACTCTGGTTACAAGTCACAGTATATTGAAGGTCAGAAGGAGAAATTTGTGAGGTATTTTTATTTTTGGGTATAACATATCAATGTGATTGGTTACTTTTTTTTTCCGTTATCTTAACTCTCTGGTTCTCGGGGGGAAAGAGACTCTATTAATCTGGATTTTGGTCAGGATGTAAGTAAAGTTTGATCAATGATTGTTCCAGTCAAGATCCGAACCTGGATATACTCTCGATAGATTTGTCATTAACGGAAGTTCATCAACCACTTGGTTCTCTCTTCAAAAAAAAAAACCACTTGGTTCTATTGGTTAATTTAAGAACATGGACTAAATTTTGATTTTACTTGCATTTTATTTTTTTGTTATATTCCACTTTAAGTTTAGGGTATGCAATGTCCAATTTGTTAACATAAGCATCTTTCAGACTAATATACAGTGGATGATGTAGGTTGGATGATTTGGATTCCAATTCGTCAATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCACAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGGTTTGAGATACCATCCATGCTCAATATGTTTTGCGTACTACGTAATGTCTCATCTCTATTTTCTTTTTTTGCCAATATGTCTTAATGTTTGTTGTAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATGTAAGTTCATTTCTTAAGACAATTCTTCTGTTTTAATTCTCCATTGCTTCTGTATCACTTATGAAGCTCGGATACTCCATAAAAGAAAAAGTGTTGGTGTCGTATCTGTATCACTATCATTCACATATTCTCTTTGGATATTTCTCGATACATGTCCAAAAAGCATCCAATTTTTCTATGGAAACTTCCCATTAAAATTGATACTCCTCATACACTTCTTGATACTTCCCGATTGGACACCAAAATTTATTTATATTCAATTTTTTGAGTTTTAATTATGATGCTTTTGTAGATATTCTGACATAGAGGTTAGAATAGATTACGCTATAGGCGGAAGCTCTAGCATTCTTGTTACCGACACGAATGATCAATGTTTCTTTCTAACTATTATTTTGTAGATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGGTATGTTCTTTCTATGATTCTTTAGAACTATATATTATCCACATGAGTCCTCTTTATAACACCTATAAAACACTCAGTTTATAAATGTAATTATAATTGATAAATATATATGTATTAGTGTCGGTGTCCTATGTTTTTCACATTAGCCATGTAGAGGTGCATGTGCCCATATCGTGTGTCTGATCTTCATATCAAAGTCGGTGCTTCATATGTCTTAAATCCATAATTTATAGAATTTTAGATTGTAGATTCACTTGATGTTGGGTTAACCTATATCATCCTTTATAAATATGCTTTGATTCTTATTGATTCAAAATATGAACACATAAGATGATAACATGTTCACACTTTGACCAAATATCCTTCTGTTTTGCTTTTCACAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGGTAAACCGAAAGTTTTTACTGTAAACATTGATTTATTGCATCTTGATTACTATTATACATACTTTAATCCTTGTGTTATAAATGCAATTGGACAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTGAGTACTGAGCAAACTAATTTGTCCTTTCTGAGATTTCTCATTGCAACTGAAAAGTGTTCCTTCTAACTCTTTATTTTCTCATTAGCATTCTGTGTATGACATATATTATTCAGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>MS.gene034223.t1

MFDSGYKSQYIEGQKEKFVRLDDLDSNSSMPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE