AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034452

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034452.t1 MTR_2g086530 98.711 388 5 0 1 388 1 388 0.0 773
MS.gene034452.t1 MTR_2g086530 99.183 367 3 0 22 388 1 367 0.0 736
MS.gene034452.t1 MTR_4g052970 66.935 372 115 2 16 385 16 381 0.0 514
MS.gene034452.t1 MTR_1g077990 61.950 318 118 2 30 345 32 348 7.32e-144 414
MS.gene034452.t1 MTR_4g084480 50.000 338 167 1 10 345 13 350 2.82e-124 364
MS.gene034452.t1 MTR_4g084480 50.000 338 167 1 10 345 6 343 3.35e-124 364
MS.gene034452.t1 MTR_4g084480 50.000 338 167 1 10 345 13 350 3.79e-124 364
MS.gene034452.t1 MTR_2g019345 48.980 343 170 3 5 345 2 341 3.34e-117 346
MS.gene034452.t1 MTR_4g010200 45.070 355 185 4 4 351 7 358 4.91e-115 340
MS.gene034452.t1 MTR_4g114850 44.932 365 187 4 29 383 26 386 4.58e-110 328
MS.gene034452.t1 MTR_4g114850 48.758 322 158 2 29 345 26 345 3.55e-109 325
MS.gene034452.t1 MTR_4g114850 48.758 322 158 2 29 345 26 345 4.12e-109 325
MS.gene034452.t1 MTR_4g069940 42.655 354 199 3 2 352 3 355 1.04e-94 291
MS.gene034452.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 2.85e-94 288
MS.gene034452.t1 MTR_3g080410 43.732 343 181 5 22 353 19 360 8.44e-94 285
MS.gene034452.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 1.62e-93 289
MS.gene034452.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 3.40e-93 288
MS.gene034452.t1 MTR_5g085580 45.092 326 174 3 24 345 20 344 3.64e-93 287
MS.gene034452.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 4.32e-93 287
MS.gene034452.t1 MTR_3g116510 42.407 349 193 4 6 351 10 353 3.68e-92 284
MS.gene034452.t1 MTR_1g056640 44.237 321 174 4 29 345 38 357 2.21e-91 283
MS.gene034452.t1 MTR_6g088885 41.547 349 197 3 5 350 6 350 5.04e-89 278
MS.gene034452.t1 MTR_1g007810 42.433 337 182 6 10 342 1 329 6.89e-87 268
MS.gene034452.t1 MTR_7g053500 40.719 334 180 7 30 351 27 354 7.56e-85 266
MS.gene034452.t1 MTR_7g026340 40.625 320 180 7 30 345 24 337 6.54e-80 253
MS.gene034452.t1 MTR_4g083500 41.617 334 188 6 28 357 43 373 1.05e-77 249
MS.gene034452.t1 MTR_8g102340 40.752 319 157 4 30 348 29 315 4.71e-77 246
MS.gene034452.t1 MTR_8g012400 36.975 357 214 7 10 356 7 362 5.44e-77 245
MS.gene034452.t1 MTR_4g088155 36.944 360 204 7 11 356 8 358 3.27e-76 243
MS.gene034452.t1 MTR_4g076490 36.288 361 200 6 2 344 54 402 1.60e-75 240
MS.gene034452.t1 MTR_8g085720 35.467 375 229 5 1 366 1 371 2.24e-74 239
MS.gene034452.t1 MTR_4g132280 38.154 325 194 3 23 341 39 362 5.13e-71 231
MS.gene034452.t1 MTR_3g083580 36.308 325 199 4 26 343 23 346 5.28e-70 228
MS.gene034452.t1 MTR_4g076470 37.278 338 188 8 12 341 3 324 2.81e-69 221
MS.gene034452.t1 MTR_4g076430 36.095 338 198 8 10 343 3 326 1.08e-67 217
MS.gene034452.t1 MTR_1g052430 38.679 318 181 8 23 336 28 335 1.30e-67 219
MS.gene034452.t1 MTR_7g053500 37.993 279 163 5 77 351 5 277 1.45e-66 216
MS.gene034452.t1 MTR_4g076440 34.718 337 204 7 10 343 3 326 4.73e-66 213
MS.gene034452.t1 MTR_4g076570 37.304 319 190 6 29 344 22 333 9.22e-66 213
MS.gene034452.t1 MTR_4g076255 36.709 316 185 6 30 343 26 328 3.34e-65 211
MS.gene034452.t1 MTR_2g034480 36.443 343 196 10 30 366 28 354 5.72e-65 211
MS.gene034452.t1 MTR_3g065460 36.628 344 209 5 8 345 5 345 7.88e-65 214
MS.gene034452.t1 MTR_1g031900 35.380 342 214 4 10 345 5 345 3.39e-64 213
MS.gene034452.t1 MTR_1g031900 35.380 342 214 4 10 345 5 345 6.00e-64 212
MS.gene034452.t1 MTR_3g116510 45.205 219 120 0 133 351 1 219 1.18e-62 204
MS.gene034452.t1 MTR_4g076500 36.538 312 186 6 30 338 79 381 3.37e-62 205
MS.gene034452.t1 MTR_8g012400 38.202 267 160 4 94 356 17 282 5.45e-61 201
MS.gene034452.t1 MTR_5g078200 35.000 340 214 3 12 345 6 344 5.77e-61 207
MS.gene034452.t1 MTR_7g081350 33.333 342 212 9 8 343 4 335 1.86e-60 199
MS.gene034452.t1 MTR_2g034470 35.168 327 194 10 30 349 31 346 3.31e-60 199
MS.gene034452.t1 MTR_5g078200 36.218 312 192 3 40 345 1 311 1.27e-58 201
MS.gene034452.t1 MTR_2g034440 35.144 313 187 9 30 336 23 325 1.62e-58 194
MS.gene034452.t1 MTR_2g034470 34.891 321 191 10 36 349 2 311 1.39e-57 191
MS.gene034452.t1 MTR_7g081370 31.778 343 204 10 8 343 4 323 3.46e-53 179
MS.gene034452.t1 MTR_5g081720 34.304 309 180 11 45 342 53 349 4.06e-47 165
MS.gene034452.t1 MTR_4g124440 32.303 356 210 16 7 345 6 347 1.59e-45 163
MS.gene034452.t1 MTR_6g032820 29.781 366 225 13 4 351 3 354 7.30e-45 160
MS.gene034452.t1 MTR_8g092070 30.793 328 198 14 30 341 35 349 7.38e-45 163
MS.gene034452.t1 MTR_7g085390 32.733 333 202 14 30 349 27 350 8.88e-45 161
MS.gene034452.t1 MTR_4g112025 31.285 358 221 12 4 346 6 353 9.55e-45 162
MS.gene034452.t1 MTR_1g010120 31.844 358 212 16 7 349 9 349 2.03e-44 160
MS.gene034452.t1 MTR_2g027560 31.343 335 205 13 30 348 29 354 6.59e-43 156
MS.gene034452.t1 MTR_3g095050 31.498 327 197 12 29 341 34 347 1.81e-39 147
MS.gene034452.t1 MTR_1g053320 29.213 356 228 12 11 353 16 360 2.14e-39 147
MS.gene034452.t1 MTR_3g108600 30.448 335 193 13 30 352 32 338 8.55e-39 145
MS.gene034452.t1 MTR_3g031770 29.042 334 202 13 30 345 28 344 2.30e-30 122
MS.gene034452.t1 MTR_4g134280 32.117 274 162 12 114 372 3 267 6.97e-30 119
MS.gene034452.t1 MTR_4g036680 42.727 110 50 5 243 351 80 177 1.79e-18 84.0
MS.gene034452.t1 MTR_4g036775 42.727 110 50 5 243 351 80 177 1.79e-18 84.0
MS.gene034452.t1 MTR_6g078180 50.000 50 25 0 90 139 5 54 4.91e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034452.t1 AT2G27500 63.277 354 121 3 26 376 26 373 1.01e-164 466
MS.gene034452.t1 AT2G27500 62.327 361 127 3 26 383 26 380 1.05e-164 467
MS.gene034452.t1 AT1G32860 53.868 349 157 2 1 345 1 349 1.20e-134 392
MS.gene034452.t1 AT2G27500 60.390 308 113 3 79 383 1 302 2.38e-134 387
MS.gene034452.t1 AT5G42100 54.969 322 140 3 30 346 28 349 6.40e-125 367
MS.gene034452.t1 AT5G42100 54.969 322 140 3 30 346 28 349 9.89e-125 367
MS.gene034452.t1 AT1G30080 47.875 353 173 3 3 344 1 353 2.18e-115 341
MS.gene034452.t1 AT1G30080 47.875 353 173 3 3 344 1 353 3.10e-115 342
MS.gene034452.t1 AT3G15800 50.311 322 154 3 29 345 43 363 2.56e-114 339
MS.gene034452.t1 AT4G18340 45.349 344 185 2 4 345 10 352 3.58e-111 330
MS.gene034452.t1 AT4G18340 45.349 344 185 2 4 345 10 352 4.14e-111 331
MS.gene034452.t1 AT2G26600 44.751 362 185 4 29 382 34 388 6.02e-109 325
MS.gene034452.t1 AT4G26830 43.804 347 185 6 1 343 1 341 4.85e-97 297
MS.gene034452.t1 AT4G26830 45.283 318 168 4 30 343 30 345 2.41e-95 293
MS.gene034452.t1 AT2G05790 44.345 336 176 6 30 356 26 359 1.13e-93 289
MS.gene034452.t1 AT5G55180 42.901 324 180 3 29 348 27 349 1.24e-93 288
MS.gene034452.t1 AT5G55180 42.901 324 180 3 29 348 27 349 1.61e-93 288
MS.gene034452.t1 AT2G26600 47.260 292 146 2 94 382 8 294 6.05e-93 281
MS.gene034452.t1 AT5G56590 44.099 322 176 3 26 343 23 344 1.06e-92 287
MS.gene034452.t1 AT2G16230 42.197 346 189 4 24 358 19 364 8.18e-92 285
MS.gene034452.t1 AT5G42720 42.566 343 190 5 8 345 6 346 1.67e-91 282
MS.gene034452.t1 AT5G24318 41.159 345 192 6 7 345 7 346 1.09e-90 278
MS.gene034452.t1 AT5G24318 41.159 345 192 6 7 345 7 346 1.84e-89 277
MS.gene034452.t1 AT5G24318 41.159 345 192 6 7 345 7 346 3.08e-89 277
MS.gene034452.t1 AT4G34480 42.286 350 192 5 1 345 1 345 6.77e-88 270
MS.gene034452.t1 AT4G34480 41.713 362 196 6 1 352 1 357 2.62e-86 271
MS.gene034452.t1 AT4G29360 42.210 353 190 6 6 348 10 358 1.04e-83 265
MS.gene034452.t1 AT3G07320 41.265 332 177 6 30 349 26 351 2.13e-83 262
MS.gene034452.t1 AT4G29360 43.168 322 179 3 30 348 28 348 1.19e-82 262
MS.gene034452.t1 AT4G14080 37.067 375 216 6 1 356 1 374 2.11e-78 249
MS.gene034452.t1 AT2G01630 40.248 323 186 3 29 345 23 344 8.30e-78 245
MS.gene034452.t1 AT2G01630 40.248 323 186 3 29 345 23 344 2.20e-77 246
MS.gene034452.t1 AT2G01630 40.373 322 185 3 30 345 24 344 2.20e-76 245
MS.gene034452.t1 AT3G23770 40.120 334 190 6 30 354 38 370 3.46e-74 238
MS.gene034452.t1 AT4G16260 37.092 337 200 6 7 341 4 330 1.05e-73 233
MS.gene034452.t1 AT3G55430 37.912 364 205 12 2 358 7 356 3.60e-72 233
MS.gene034452.t1 AT1G11820 38.507 335 198 4 30 357 45 378 4.42e-72 230
MS.gene034452.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene034452.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene034452.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene034452.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene034452.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene034452.t1 AT1G11820 39.375 320 187 3 30 343 45 363 9.98e-71 229
MS.gene034452.t1 AT1G11820 39.375 320 187 3 30 343 45 363 1.83e-70 230
MS.gene034452.t1 AT2G39640 40.122 329 183 6 30 356 31 347 2.21e-69 228
MS.gene034452.t1 AT3G46570 39.017 346 196 10 9 345 5 344 6.00e-69 221
MS.gene034452.t1 AT4G16260 37.662 308 182 5 36 341 2 301 6.85e-69 220
MS.gene034452.t1 AT3G57260 35.608 337 200 10 10 343 14 336 2.80e-66 214
MS.gene034452.t1 AT3G57260 35.608 337 200 10 10 343 30 352 5.38e-66 214
MS.gene034452.t1 AT3G57270 35.952 331 196 9 20 343 16 337 5.48e-65 211
MS.gene034452.t1 AT4G34480 44.672 244 130 1 114 352 3 246 2.91e-63 207
MS.gene034452.t1 AT3G61810 34.365 323 194 7 30 343 56 369 2.99e-62 204
MS.gene034452.t1 AT5G24318 45.024 211 111 2 136 345 1 207 1.41e-61 201
MS.gene034452.t1 AT1G66250 35.583 326 202 4 29 348 32 355 4.15e-61 205
MS.gene034452.t1 AT3G57240 33.968 315 191 8 30 341 36 336 1.07e-57 192
MS.gene034452.t1 AT1G77780 33.333 342 212 12 7 343 2 332 1.45e-57 192
MS.gene034452.t1 AT1G77790 33.526 346 214 11 8 346 8 344 1.80e-57 191
MS.gene034452.t1 AT5G20390 34.311 341 210 10 8 343 12 343 1.87e-56 189
MS.gene034452.t1 AT5G20340 33.051 354 210 12 6 343 11 353 3.41e-53 180
MS.gene034452.t1 AT5G20330 32.857 350 208 10 9 343 7 344 4.09e-52 177
MS.gene034452.t1 AT1G33220 30.814 344 212 10 6 343 11 334 3.86e-45 159
MS.gene034452.t1 AT1G64760 31.198 359 222 13 3 348 1 347 6.20e-45 162
MS.gene034452.t1 AT1G64760 31.198 359 222 13 3 348 1 347 6.20e-45 162
MS.gene034452.t1 AT2G19440 32.121 330 207 10 29 348 21 343 9.36e-45 161
MS.gene034452.t1 AT2G19440 32.121 330 207 10 29 348 21 343 9.36e-45 161
MS.gene034452.t1 AT3G55780 32.492 317 194 12 42 347 48 355 1.14e-43 156
MS.gene034452.t1 AT5G18220 29.499 339 223 10 11 341 13 343 2.93e-43 157
MS.gene034452.t1 AT5G64790 30.056 356 219 13 10 351 14 353 3.74e-41 151
MS.gene034452.t1 AT3G04010 31.519 349 219 13 12 348 15 355 5.79e-41 151
MS.gene034452.t1 AT5G58480 31.845 336 202 12 30 349 27 351 4.99e-40 148
MS.gene034452.t1 AT5G20560 29.970 337 218 10 6 335 11 336 2.09e-39 144
MS.gene034452.t1 AT4G31140 28.895 353 231 13 2 345 5 346 2.48e-39 146
MS.gene034452.t1 AT5G20870 32.320 362 212 16 12 350 10 361 2.74e-39 147
MS.gene034452.t1 AT3G24330 29.379 354 226 13 10 348 19 363 1.66e-37 142
MS.gene034452.t1 AT4G17180 30.816 331 205 13 30 348 26 344 7.95e-37 139
MS.gene034452.t1 AT5G58090 28.875 329 217 11 30 347 23 345 8.35e-36 137

Find 63 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGCAATGGTTTGGATTT+TGG 0.082492 2.3:-12479563 MS.gene034452:CDS
TACATTAATTGAAGTGATTT+TGG 0.138128 2.3:+12479304 None:intergenic
ATGCTGTTTCTCACCGTTTC+AGG 0.170134 2.3:-12479911 MS.gene034452:intron
TCTCTACATTCTTACATTCT+TGG 0.176614 2.3:-12478290 MS.gene034452:CDS
ACCCATGATTGTGCCTTAAT+AGG 0.222049 2.3:+12479359 None:intergenic
TACATTCTTGGTGTTTGTTT+GGG 0.256184 2.3:-12478278 MS.gene034452:CDS
TTACATTCTTGGTGTTTGTT+TGG 0.257209 2.3:-12478279 MS.gene034452:CDS
GAGAATGTTGTTAGAACATT+TGG 0.274660 2.3:+12479437 None:intergenic
TGCTGTTTACGCTGCTATAA+AGG 0.295122 2.3:-12478898 MS.gene034452:CDS
TATAATGCACTTGTTAATCT+TGG 0.303970 2.3:-12479212 MS.gene034452:CDS
TCAAATGTTGAATTCATGAT+AGG 0.342689 2.3:-12479410 MS.gene034452:CDS
CCCATGATTGTGCCTTAATA+GGG 0.349920 2.3:+12479360 None:intergenic
TTCAATCCAAGGTTATAAAC+TGG 0.351246 2.3:+12478615 None:intergenic
TTGCCTTCAATAATCATTCC+TGG 0.356449 2.3:+12478564 None:intergenic
AATTCATTCCCTCCTTCATC+TGG 0.362933 2.3:-12479137 MS.gene034452:CDS
AAGTATGAAAGACCCTATTA+AGG 0.379469 2.3:-12479372 MS.gene034452:CDS
TGCAGGAGTTCCTTGTTTCA+TGG 0.387844 2.3:+12478746 None:intergenic
TTATACACTGATTTCATTGC+AGG 0.395898 2.3:+12479230 None:intergenic
TAAATATCAATAGGAATAGA+AGG 0.399395 2.3:+12478720 None:intergenic
GTTTATGCAAAGCAATGGTT+TGG 0.402259 2.3:-12479570 MS.gene034452:CDS
AATGTAGAGAACAAAATTGA+TGG 0.411079 2.3:+12478303 None:intergenic
GCGAAAACATAAATATCAAT+AGG 0.415882 2.3:+12478711 None:intergenic
CCTGCATCAGAGAGAAACTA+TGG 0.421805 2.3:-12478660 MS.gene034452:CDS
AGTGAAGCTCCAACCTCATC+AGG 0.429944 2.3:+12478813 None:intergenic
CTGATTTCATTGCAGGAAGA+AGG 0.441600 2.3:+12479237 None:intergenic
ATCATAATAATTTGTTGAAG+AGG 0.447117 2.3:-12478773 MS.gene034452:CDS
TTTCCAGGAATGATTATTGA+AGG 0.454929 2.3:-12478567 MS.gene034452:CDS
TCATCAGGATCACCTTTAGA+AGG 0.456945 2.3:+12478828 None:intergenic
GGTACTCCAGTTTATAACCT+TGG 0.457524 2.3:-12478621 MS.gene034452:CDS
CCCTATTAAGGCACAATCAT+GGG 0.483365 2.3:-12479360 MS.gene034452:CDS
ACCCTATTAAGGCACAATCA+TGG 0.484483 2.3:-12479361 MS.gene034452:CDS
TCTCTAAATGCACCAGATGA+AGG 0.485251 2.3:+12479125 None:intergenic
TTTAACGAAGATTTGAAGAC+TGG 0.490478 2.3:-12478684 MS.gene034452:CDS
GAGACAGGTTGGCCTTCTAA+AGG 0.497141 2.3:-12478840 MS.gene034452:CDS
TCATAATGCAAATTTGTAGC+AGG 0.507641 2.3:+12478945 None:intergenic
CTGGAAAATAACCTCCTTGA+GGG 0.515738 2.3:+12478583 None:intergenic
AGTAAAAGACTAACCTGAAA+CGG 0.517656 2.3:+12479898 None:intergenic
TTTAGAAGGCCAACCTGTCT+CGG 0.517858 2.3:+12478842 None:intergenic
GAGGTTAAGATTTCCGAGAC+AGG 0.525013 2.3:-12478855 MS.gene034452:CDS
CCATAGTTTCTCTCTGATGC+AGG 0.528755 2.3:+12478660 None:intergenic
AAAATCACTTCAATTAATGT+AGG 0.530035 2.3:-12479302 MS.gene034452:CDS
TTATAAACTGGAGTACCATC+AGG 0.532992 2.3:+12478627 None:intergenic
TGCGCTTGAAAGCTAAGAAG+TGG 0.540232 2.3:+12479080 None:intergenic
TATGGTCTTTATTATCCTGA+TGG 0.543216 2.3:-12478642 MS.gene034452:CDS
CATTATCACTCTGAAGAAGA+AGG 0.546977 2.3:+12479931 None:intergenic
TAGTTTAATGAAACATGTTG+TGG 0.553999 2.3:+12479002 None:intergenic
GTCTTGTTTCAACCGAACCC+TGG 0.554364 2.3:-12478978 MS.gene034452:CDS
GGATCAATAGAACCAGGGTT+CGG 0.558568 2.3:+12478966 None:intergenic
AAAGGTGATCCTGATGAGGT+TGG 0.562864 2.3:-12478822 MS.gene034452:CDS
AAGAGGATTGCCATGAAACA+AGG 0.577245 2.3:-12478756 MS.gene034452:CDS
GATTTGTTTATGCAAAGCAA+TGG 0.583237 2.3:-12479575 MS.gene034452:CDS
GTAGCAGGATCAATAGAACC+AGG 0.591805 2.3:+12478960 None:intergenic
TTAAGATTTCCGAGACAGGT+TGG 0.593135 2.3:-12478851 MS.gene034452:CDS
CTAAATGCACCAGATGAAGG+AGG 0.616012 2.3:+12479128 None:intergenic
TACGCTGCTATAAAGGCGGT+AGG 0.619683 2.3:-12478891 MS.gene034452:CDS
TTCTAAAGGTGATCCTGATG+AGG 0.626238 2.3:-12478826 MS.gene034452:CDS
CCTGGAAAATAACCTCCTTG+AGG 0.629807 2.3:+12478582 None:intergenic
TAAATGCACCAGATGAAGGA+GGG 0.634421 2.3:+12479129 None:intergenic
ACTGCTACACGTGAATGAGA+AGG 0.642551 2.3:+12479509 None:intergenic
ACGCTGCTATAAAGGCGGTA+GGG 0.646566 2.3:-12478890 MS.gene034452:CDS
TAGCAGGATCAATAGAACCA+GGG 0.673870 2.3:+12478961 None:intergenic
GGTAGGGCATAGCGATATCG+AGG 0.719545 2.3:-12478874 MS.gene034452:CDS
TGTTTACGCTGCTATAAAGG+CGG 0.719749 2.3:-12478895 MS.gene034452:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGAAATAAAAATAATATG+TGG + chr2.3:12478361-12478380 None:intergenic 10.0%
!! AAACTAAAATATACTTACAT+TGG + chr2.3:12479669-12479688 None:intergenic 15.0%
!! AAAATCACTTCAATTAATGT+AGG - chr2.3:12478903-12478922 MS.gene034452:CDS 20.0%
!! TAAATATCAATAGGAATAGA+AGG + chr2.3:12479488-12479507 None:intergenic 20.0%
!! TTCAACAAAATTGTTAAAAC+AGG + chr2.3:12478538-12478557 None:intergenic 20.0%
!!! AAAGAGTAGTTTTTATTTTC+TGG + chr2.3:12478461-12478480 None:intergenic 20.0%
!!! AAGAGTAGTTTTTATTTTCT+GGG + chr2.3:12478460-12478479 None:intergenic 20.0%
!!! ATCATAATAATTTGTTGAAG+AGG - chr2.3:12479432-12479451 MS.gene034452:CDS 20.0%
!!! TACATTAATTGAAGTGATTT+TGG + chr2.3:12478904-12478923 None:intergenic 20.0%
! AATGTAGAGAACAAAATTGA+TGG + chr2.3:12479905-12479924 None:intergenic 25.0%
! GCGAAAACATAAATATCAAT+AGG + chr2.3:12479497-12479516 None:intergenic 25.0%
! TAGTTTAATGAAACATGTTG+TGG + chr2.3:12479206-12479225 None:intergenic 25.0%
! TCAAATGTTGAATTCATGAT+AGG - chr2.3:12478795-12478814 MS.gene034452:CDS 25.0%
!! AATGTAGGAAATGAAGTTTT+AGG - chr2.3:12478918-12478937 MS.gene034452:CDS 25.0%
!! TATAATGCACTTGTTAATCT+TGG - chr2.3:12478993-12479012 MS.gene034452:CDS 25.0%
!!! TATCCTTTTTTTGCATACAA+AGG - chr2.3:12479176-12479195 MS.gene034452:CDS 25.0%
!!! TCCAATTTTTGTTAATTTGC+AGG - chr2.3:12479873-12479892 MS.gene034452:intron 25.0%
!!! TTGGATTTTGGAATAAACTA+TGG - chr2.3:12478654-12478673 MS.gene034452:CDS 25.0%
AAGTATGAAAGACCCTATTA+AGG - chr2.3:12478833-12478852 MS.gene034452:CDS 30.0%
AGTAAAAGACTAACCTGAAA+CGG + chr2.3:12478310-12478329 None:intergenic 30.0%
GAGAATGTTGTTAGAACATT+TGG + chr2.3:12478771-12478790 None:intergenic 30.0%
GCCTGCAAATTAACAAAAAT+TGG + chr2.3:12479877-12479896 None:intergenic 30.0%
TATGGTCTTTATTATCCTGA+TGG - chr2.3:12479563-12479582 MS.gene034452:CDS 30.0%
TCACCTTTGTATGCAAAAAA+AGG + chr2.3:12479182-12479201 None:intergenic 30.0%
TCATAATGCAAATTTGTAGC+AGG + chr2.3:12479263-12479282 None:intergenic 30.0%
TCTCTACATTCTTACATTCT+TGG - chr2.3:12479915-12479934 MS.gene034452:CDS 30.0%
TTCAATCCAAGGTTATAAAC+TGG + chr2.3:12479593-12479612 None:intergenic 30.0%
TTTAACGAAGATTTGAAGAC+TGG - chr2.3:12479521-12479540 MS.gene034452:CDS 30.0%
TTTCCAGGAATGATTATTGA+AGG - chr2.3:12479638-12479657 MS.gene034452:intron 30.0%
! GATTTGTTTATGCAAAGCAA+TGG - chr2.3:12478630-12478649 MS.gene034452:CDS 30.0%
!! ATAAGTGCTGAATATATCTG+TGG - chr2.3:12478394-12478413 MS.gene034452:intron 30.0%
!! GGATAAGCATTGATCAAAAA+AGG + chr2.3:12479161-12479180 None:intergenic 30.0%
!! TACATTCTTGGTGTTTGTTT+GGG - chr2.3:12479927-12479946 MS.gene034452:CDS 30.0%
!! TATTTTCTGGGTATTGTAAG+CGG + chr2.3:12478448-12478467 None:intergenic 30.0%
!! TTACATTCTTGGTGTTTGTT+TGG - chr2.3:12479926-12479945 MS.gene034452:CDS 30.0%
!! TTATACACTGATTTCATTGC+AGG + chr2.3:12478978-12478997 None:intergenic 30.0%
!!! ATTGAAGTGATTTTGGTTTG+TGG + chr2.3:12478897-12478916 None:intergenic 30.0%
AATCTTAGACCTTCCGATTA+TGG - chr2.3:12479730-12479749 MS.gene034452:intron 35.0%
GACATCAAATGAGCCATAAT+CGG + chr2.3:12479746-12479765 None:intergenic 35.0%
GTTTATGCAAAGCAATGGTT+TGG - chr2.3:12478635-12478654 MS.gene034452:CDS 35.0%
TTGAAAAATCAAGTCCCTCA+AGG - chr2.3:12479608-12479627 MS.gene034452:intron 35.0%
TTGCCTTCAATAATCATTCC+TGG + chr2.3:12479644-12479663 None:intergenic 35.0%
! ATAGGAATAGAAGGTTTTGC+AGG + chr2.3:12479479-12479498 None:intergenic 35.0%
! CATTATCACTCTGAAGAAGA+AGG + chr2.3:12478277-12478296 None:intergenic 35.0%
! TTATAAACTGGAGTACCATC+AGG + chr2.3:12479581-12479600 None:intergenic 35.0%
!! CAAAGCAATGGTTTGGATTT+TGG - chr2.3:12478642-12478661 MS.gene034452:CDS 35.0%
!! GGACTTGATTTTTCAATCCA+AGG + chr2.3:12479604-12479623 None:intergenic 35.0%
!!! AAGTGATTTTGGTTTGTGGA+AGG + chr2.3:12478893-12478912 None:intergenic 35.0%
!!! ATTTTGGTTTGTGGAAGGTA+AGG + chr2.3:12478888-12478907 None:intergenic 35.0%
AAAAATCAAGTCCCTCAAGG+AGG - chr2.3:12479611-12479630 MS.gene034452:intron 40.0%
AAGAGGATTGCCATGAAACA+AGG - chr2.3:12479449-12479468 MS.gene034452:CDS 40.0%
AATTCATTCCCTCCTTCATC+TGG - chr2.3:12479068-12479087 MS.gene034452:CDS 40.0%
ACCAATCGAGTAGTCTGTTT+CGG - chr2.3:12479775-12479794 MS.gene034452:intron 40.0%
ACCCATGATTGTGCCTTAAT+AGG + chr2.3:12478849-12478868 None:intergenic 40.0%
ACCCTATTAAGGCACAATCA+TGG - chr2.3:12478844-12478863 MS.gene034452:CDS 40.0%
CCCATGATTGTGCCTTAATA+GGG + chr2.3:12478848-12478867 None:intergenic 40.0%
CCCTATTAAGGCACAATCAT+GGG - chr2.3:12478845-12478864 MS.gene034452:CDS 40.0%
CTGATTTCATTGCAGGAAGA+AGG + chr2.3:12478971-12478990 None:intergenic 40.0%
CTGGAAAATAACCTCCTTGA+GGG + chr2.3:12479625-12479644 None:intergenic 40.0%
GGTACTCCAGTTTATAACCT+TGG - chr2.3:12479584-12479603 MS.gene034452:intron 40.0%
TAAATGCACCAGATGAAGGA+GGG + chr2.3:12479079-12479098 None:intergenic 40.0%
TAGCAGGATCAATAGAACCA+GGG + chr2.3:12479247-12479266 None:intergenic 40.0%
TCAAATGAGCCATAATCGGA+AGG + chr2.3:12479742-12479761 None:intergenic 40.0%
TCATCAGGATCACCTTTAGA+AGG + chr2.3:12479380-12479399 None:intergenic 40.0%
TCTCTAAATGCACCAGATGA+AGG + chr2.3:12479083-12479102 None:intergenic 40.0%
TGGTATGAATCGTGACAGTA+AGG - chr2.3:12478414-12478433 MS.gene034452:intron 40.0%
TGTTTACGCTGCTATAAAGG+CGG - chr2.3:12479310-12479329 MS.gene034452:CDS 40.0%
TTAAGATTTCCGAGACAGGT+TGG - chr2.3:12479354-12479373 MS.gene034452:CDS 40.0%
! TGCTGTTTACGCTGCTATAA+AGG - chr2.3:12479307-12479326 MS.gene034452:CDS 40.0%
! TTCTAAAGGTGATCCTGATG+AGG - chr2.3:12479379-12479398 MS.gene034452:CDS 40.0%
AAAGGTGATCCTGATGAGGT+TGG - chr2.3:12479383-12479402 MS.gene034452:CDS 45.0%
ACTGCTACACGTGAATGAGA+AGG + chr2.3:12478699-12478718 None:intergenic 45.0%
CCATAGTTTCTCTCTGATGC+AGG + chr2.3:12479548-12479567 None:intergenic 45.0%
CCTGCATCAGAGAGAAACTA+TGG - chr2.3:12479545-12479564 MS.gene034452:CDS 45.0%
CCTGGAAAATAACCTCCTTG+AGG + chr2.3:12479626-12479645 None:intergenic 45.0%
CTAAATGCACCAGATGAAGG+AGG + chr2.3:12479080-12479099 None:intergenic 45.0%
GAGGTTAAGATTTCCGAGAC+AGG - chr2.3:12479350-12479369 MS.gene034452:CDS 45.0%
GGATCAATAGAACCAGGGTT+CGG + chr2.3:12479242-12479261 None:intergenic 45.0%
GTAGCAGGATCAATAGAACC+AGG + chr2.3:12479248-12479267 None:intergenic 45.0%
TCCGAAACAGACTACTCGAT+TGG + chr2.3:12479779-12479798 None:intergenic 45.0%
TGCGCTTGAAAGCTAAGAAG+TGG + chr2.3:12479128-12479147 None:intergenic 45.0%
TTTAGAAGGCCAACCTGTCT+CGG + chr2.3:12479366-12479385 None:intergenic 45.0%
! TGCAGGAGTTCCTTGTTTCA+TGG + chr2.3:12479462-12479481 None:intergenic 45.0%
!! ATGCTGTTTCTCACCGTTTC+AGG - chr2.3:12478294-12478313 MS.gene034452:CDS 45.0%
!! CCTCAAGGAGGTTATTTTCC+AGG - chr2.3:12479623-12479642 MS.gene034452:intron 45.0%
ACGCTGCTATAAAGGCGGTA+GGG - chr2.3:12479315-12479334 MS.gene034452:CDS 50.0%
ACTACTCGATTGGTGAGCTG+AGG + chr2.3:12479769-12479788 None:intergenic 50.0%
AGTGAAGCTCCAACCTCATC+AGG + chr2.3:12479395-12479414 None:intergenic 50.0%
CTACTCGATTGGTGAGCTGA+GGG + chr2.3:12479768-12479787 None:intergenic 50.0%
TACGCTGCTATAAAGGCGGT+AGG - chr2.3:12479314-12479333 MS.gene034452:CDS 50.0%
!! GAGACAGGTTGGCCTTCTAA+AGG - chr2.3:12479365-12479384 MS.gene034452:CDS 50.0%
!! GTCTTGTTTCAACCGAACCC+TGG - chr2.3:12479227-12479246 MS.gene034452:CDS 50.0%
GGTAGGGCATAGCGATATCG+AGG - chr2.3:12479331-12479350 MS.gene034452:CDS 55.0%
! GGGTATTGTAAGCGGCAGAG+TGG + chr2.3:12478440-12478459 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 12478261 12479966 12478261 ID=MS.gene034452
chr2.3 mRNA 12478261 12479966 12478261 ID=MS.gene034452.t1;Parent=MS.gene034452
chr2.3 exon 12479912 12479966 12479912 ID=MS.gene034452.t1.exon1;Parent=MS.gene034452.t1
chr2.3 CDS 12479912 12479966 12479912 ID=cds.MS.gene034452.t1;Parent=MS.gene034452.t1
chr2.3 exon 12478557 12479596 12478557 ID=MS.gene034452.t1.exon2;Parent=MS.gene034452.t1
chr2.3 CDS 12478557 12479596 12478557 ID=cds.MS.gene034452.t1;Parent=MS.gene034452.t1
chr2.3 exon 12478261 12478332 12478261 ID=MS.gene034452.t1.exon3;Parent=MS.gene034452.t1
chr2.3 CDS 12478261 12478332 12478261 ID=cds.MS.gene034452.t1;Parent=MS.gene034452.t1
Gene Sequence

>MS.gene034452

ATGGCAATCTCTTCCTTCTTCTTCAGAGTGATAATGCTGTTTCTCACCGTTTCAGGTTAGTCTTTTACTTCTTCTGCGTGCATGATTCTCTTTCATACCACATATTATTTTTATTTCATAACGAATCGAAGCGATAAGTGCTGAATATATCTGTGGTATGAATCGTGACAGTAAGGCCACTCTGCCGCTTACAATACCCAGAAAATAAAAACTACTCTTTTATTGTCAAGTAATCTTTATTTTGCACTTTTTTTTGCTTGAATTTGATATTTTACCTGTTTTAACAATTTTGTTGAATTAGATATTTGTTCTACTAAATTAAATTTAATGAACAAATTTTGATATTTCAAACTTTTTGTTCTTTTTTCAGATTTGTTTATGCAAAGCAATGGTTTGGATTTTGGAATAAACTATGGACAAATAGCAAACAATTTACCTTCTCATTCACGTGTAGCAGTTCTTATAAAATCATTGAATGTGAGCAGAATCAAACTCTATGATGCTGATCCAAATGTTCTAACAACATTCTCAAATTCAAATGTTGAATTCATGATAGGACTTAATGATCTTCAAAGTATGAAAGACCCTATTAAGGCACAATCATGGGTTCAACAAAATGTTCAACCTTACCTTCCACAAACCAAAATCACTTCAATTAATGTAGGAAATGAAGTTTTAGGTAACAATGATATAAACAGTTATAATAACCTTCTTCCTGCAATGAAATCAGTGTATAATGCACTTGTTAATCTTGGATTATCACAACAAGTTACTGTCACAACTTCACATTCTTTCATCATTATGTCAAATTCATTCCCTCCTTCATCTGGTGCATTTAGAGAAGATTTGATACAATATATTCAACCACTTCTTAGCTTTCAAGCGCAAATCAAATCACCTTTTTTGATCAATGCTTATCCTTTTTTTGCATACAAAGGTGATCCACAACATGTTTCATTAAACTATGTCTTGTTTCAACCGAACCCTGGTTCTATTGATCCTGCTACAAATTTGCATTATGATAACATGTTGTATGCTCAAATCGATGCTGTTTACGCTGCTATAAAGGCGGTAGGGCATAGCGATATCGAGGTTAAGATTTCCGAGACAGGTTGGCCTTCTAAAGGTGATCCTGATGAGGTTGGAGCTTCACTACAGAATGCAGAAATTTATCATAATAATTTGTTGAAGAGGATTGCCATGAAACAAGGAACTCCTGCAAAACCTTCTATTCCTATTGATATTTATGTTTTCGCACTTTTTAACGAAGATTTGAAGACTGGTCCTGCATCAGAGAGAAACTATGGTCTTTATTATCCTGATGGTACTCCAGTTTATAACCTTGGATTGAAAAATCAAGTCCCTCAAGGAGGTTATTTTCCAGGAATGATTATTGAAGGCAAATCCAATGTAAGTATATTTTAGTTTGTGTTAGTAAATTTGTAGATTATGTTAGTTTTCAGAGTTTGAATCTTAGACCTTCCGATTATGGCTCATTTGATGTCCCTCAGCTCACCAATCGAGTAGTCTGTTTCGGATATTATCTTTTGTAATTAACATTTTTGTAGAGTATATTACAATATGACTGAGTAATATTTGATGTATCTAACTCTCCAATTTTTGTTAATTTGCAGGCTTTGTCCATCAATTTTGTTCTCTACATTCTTACATTCTTGGTGTTTGTTTGGGAGCTTTCAATATTGTGA
Protein sequence

>MS.gene034452.t1

MAISSFFFRVIMLFLTVSDLFMQSNGLDFGINYGQIANNLPSHSRVAVLIKSLNVSRIKLYDADPNVLTTFSNSNVEFMIGLNDLQSMKDPIKAQSWVQQNVQPYLPQTKITSINVGNEVLGNNDINSYNNLLPAMKSVYNALVNLGLSQQVTVTTSHSFIIMSNSFPPSSGAFREDLIQYIQPLLSFQAQIKSPFLINAYPFFAYKGDPQHVSLNYVLFQPNPGSIDPATNLHYDNMLYAQIDAVYAAIKAVGHSDIEVKISETGWPSKGDPDEVGASLQNAEIYHNNLLKRIAMKQGTPAKPSIPIDIYVFALFNEDLKTGPASERNYGLYYPDGTPVYNLGLKNQVPQGGYFPGMIIEGKSNALSINFVLYILTFLVFVWELSIL