AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03848

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03848.t1 MTR_4g052970 97.742 310 7 0 1 310 53 362 0.0 625
MS.gene03848.t1 MTR_2g086530 65.823 316 100 2 1 310 32 345 9.99e-153 432
MS.gene03848.t1 MTR_2g086530 66.032 315 99 2 1 309 53 365 1.34e-152 433
MS.gene03848.t1 MTR_1g077990 58.446 296 122 1 1 296 55 349 2.21e-129 374
MS.gene03848.t1 MTR_4g084480 52.013 298 143 0 1 298 56 353 1.00e-115 339
MS.gene03848.t1 MTR_4g084480 52.013 298 143 0 1 298 49 346 1.47e-115 338
MS.gene03848.t1 MTR_4g084480 52.013 298 143 0 1 298 56 353 1.60e-115 339
MS.gene03848.t1 MTR_2g019345 51.186 295 143 1 1 295 48 341 1.29e-110 327
MS.gene03848.t1 MTR_4g010200 45.752 306 165 1 2 306 58 363 9.88e-100 298
MS.gene03848.t1 MTR_4g114850 47.297 296 155 1 3 297 52 347 3.95e-99 296
MS.gene03848.t1 MTR_4g114850 47.297 296 155 1 3 297 52 347 7.19e-99 296
MS.gene03848.t1 MTR_4g114850 47.297 296 155 1 3 297 52 347 1.30e-98 295
MS.gene03848.t1 MTR_3g080410 46.557 305 151 5 3 298 52 353 4.74e-88 268
MS.gene03848.t1 MTR_4g069940 45.085 295 161 1 2 295 54 348 1.18e-87 270
MS.gene03848.t1 MTR_1g007810 43.986 291 158 2 3 292 43 329 9.76e-87 264
MS.gene03848.t1 MTR_1g022160 43.878 294 163 2 5 296 52 345 6.00e-86 264
MS.gene03848.t1 MTR_1g056640 46.939 294 151 4 5 295 66 357 8.06e-86 266
MS.gene03848.t1 MTR_1g022160 43.878 294 163 2 5 296 52 345 3.54e-85 264
MS.gene03848.t1 MTR_1g022160 43.878 294 163 2 5 296 52 345 4.47e-85 264
MS.gene03848.t1 MTR_3g116510 43.537 294 165 1 3 295 54 347 5.19e-85 263
MS.gene03848.t1 MTR_1g022160 44.027 293 162 2 5 295 52 344 6.07e-85 263
MS.gene03848.t1 MTR_5g085580 46.128 297 153 4 3 295 51 344 2.94e-84 261
MS.gene03848.t1 MTR_6g088885 44.040 302 161 3 3 300 53 350 3.51e-84 262
MS.gene03848.t1 MTR_8g102340 48.047 256 132 1 42 297 60 314 1.06e-79 250
MS.gene03848.t1 MTR_7g053500 38.585 311 185 5 1 306 50 359 1.27e-78 247
MS.gene03848.t1 MTR_7g026340 42.177 294 163 5 3 295 50 337 8.77e-78 244
MS.gene03848.t1 MTR_4g088155 37.829 304 181 5 1 297 47 349 3.76e-72 230
MS.gene03848.t1 MTR_8g012400 37.255 306 186 5 1 301 53 357 7.91e-71 226
MS.gene03848.t1 MTR_7g053500 39.286 280 164 5 32 306 4 282 8.67e-71 224
MS.gene03848.t1 MTR_4g083500 41.837 294 167 4 3 295 71 361 8.22e-70 226
MS.gene03848.t1 MTR_1g031900 38.000 300 181 3 2 297 49 347 9.15e-69 222
MS.gene03848.t1 MTR_1g031900 38.000 300 181 3 2 297 49 347 9.61e-69 222
MS.gene03848.t1 MTR_8g085720 38.033 305 184 3 3 303 52 355 1.31e-68 222
MS.gene03848.t1 MTR_4g132280 38.796 299 178 3 3 297 71 368 2.50e-68 222
MS.gene03848.t1 MTR_3g065460 38.870 301 175 4 3 297 50 347 9.98e-68 219
MS.gene03848.t1 MTR_4g076490 38.176 296 169 5 3 297 123 405 2.98e-65 211
MS.gene03848.t1 MTR_4g076255 38.488 291 166 5 3 293 51 328 5.11e-64 205
MS.gene03848.t1 MTR_1g052430 39.806 309 168 9 3 308 58 351 6.98e-64 207
MS.gene03848.t1 MTR_3g116510 46.009 213 115 0 83 295 1 213 1.35e-63 204
MS.gene03848.t1 MTR_4g076470 37.586 290 166 6 3 291 49 324 1.61e-63 204
MS.gene03848.t1 MTR_8g012400 38.182 275 164 5 32 301 4 277 2.32e-63 205
MS.gene03848.t1 MTR_5g078200 36.545 301 182 4 3 297 16 313 1.54e-62 208
MS.gene03848.t1 MTR_5g078200 36.545 301 182 4 3 297 49 346 3.14e-62 208
MS.gene03848.t1 MTR_4g076440 37.543 293 170 6 3 295 49 328 1.36e-61 199
MS.gene03848.t1 MTR_4g076570 38.908 293 171 5 3 294 48 333 1.96e-61 199
MS.gene03848.t1 MTR_3g083580 35.034 294 185 4 3 291 52 344 5.10e-61 202
MS.gene03848.t1 MTR_2g034470 38.796 299 171 8 2 297 20 309 3.30e-59 192
MS.gene03848.t1 MTR_2g034470 38.796 299 171 8 2 297 55 344 9.88e-59 192
MS.gene03848.t1 MTR_4g076500 37.762 286 168 5 3 287 104 380 4.76e-58 191
MS.gene03848.t1 MTR_4g076430 36.610 295 170 8 3 295 49 328 6.32e-58 189
MS.gene03848.t1 MTR_7g081350 33.793 290 182 6 3 291 53 333 2.74e-57 188
MS.gene03848.t1 MTR_2g034440 38.276 290 167 8 2 288 47 327 6.77e-57 187
MS.gene03848.t1 MTR_2g034480 35.906 298 181 7 2 297 52 341 1.54e-56 187
MS.gene03848.t1 MTR_7g081370 32.759 290 172 7 3 291 54 321 3.10e-50 169
MS.gene03848.t1 MTR_8g092070 33.003 303 188 10 3 297 60 355 4.36e-50 175
MS.gene03848.t1 MTR_1g010120 34.069 317 181 12 3 309 52 350 7.42e-47 164
MS.gene03848.t1 MTR_5g081720 35.354 297 179 9 1 292 61 349 3.30e-45 157
MS.gene03848.t1 MTR_2g027560 34.106 302 184 10 3 295 54 349 1.68e-43 155
MS.gene03848.t1 MTR_7g085390 33.010 309 185 13 3 299 52 350 5.95e-42 151
MS.gene03848.t1 MTR_4g124440 30.492 305 193 11 3 297 54 349 2.56e-41 150
MS.gene03848.t1 MTR_6g032820 33.333 303 178 12 3 291 52 344 1.50e-40 147
MS.gene03848.t1 MTR_3g108600 32.667 300 170 10 3 295 57 331 6.88e-40 145
MS.gene03848.t1 MTR_1g053320 32.343 303 189 10 5 297 58 354 9.17e-40 145
MS.gene03848.t1 MTR_4g112025 33.443 305 184 12 5 298 59 355 3.18e-39 144
MS.gene03848.t1 MTR_3g095050 30.892 314 192 15 2 301 59 361 1.23e-38 142
MS.gene03848.t1 MTR_3g031770 30.820 305 193 11 2 297 52 347 1.45e-34 131
MS.gene03848.t1 MTR_4g134280 34.340 265 148 11 64 309 3 260 2.48e-31 120
MS.gene03848.t1 MTR_6g078180 76.000 50 12 0 40 89 5 54 1.37e-20 84.0
MS.gene03848.t1 MTR_4g036775 38.235 102 56 4 193 293 80 175 4.65e-15 73.2
MS.gene03848.t1 MTR_4g036680 38.235 102 56 4 193 293 80 175 4.83e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03848.t1 AT2G27500 63.754 309 111 1 1 308 53 361 8.12e-157 444
MS.gene03848.t1 AT2G27500 63.754 309 111 1 1 308 53 361 8.69e-157 443
MS.gene03848.t1 AT2G27500 62.807 285 105 1 27 310 1 285 4.45e-142 403
MS.gene03848.t1 AT1G32860 55.892 297 129 1 1 295 53 349 7.24e-126 366
MS.gene03848.t1 AT5G42100 53.512 299 136 2 1 296 51 349 2.87e-115 339
MS.gene03848.t1 AT5G42100 53.512 299 136 2 1 296 51 349 3.93e-115 339
MS.gene03848.t1 AT4G18340 49.153 295 150 0 1 295 58 352 1.94e-110 325
MS.gene03848.t1 AT4G18340 49.153 295 150 0 1 295 58 352 2.66e-110 326
MS.gene03848.t1 AT1G30080 49.320 294 149 0 1 294 60 353 1.41e-106 316
MS.gene03848.t1 AT1G30080 49.320 294 149 0 1 294 60 353 2.52e-106 316
MS.gene03848.t1 AT3G15800 48.993 298 150 2 2 297 68 365 2.23e-104 311
MS.gene03848.t1 AT2G26600 45.424 295 160 1 2 295 59 353 3.79e-99 297
MS.gene03848.t1 AT4G26830 47.440 293 150 3 3 293 55 345 1.23e-93 285
MS.gene03848.t1 AT4G26830 47.440 293 150 3 3 293 51 341 1.35e-93 285
MS.gene03848.t1 AT2G26600 47.082 257 136 0 39 295 3 259 1.57e-91 274
MS.gene03848.t1 AT5G55180 45.455 297 159 3 3 297 53 348 4.87e-88 271
MS.gene03848.t1 AT5G55180 45.455 297 159 3 3 297 53 348 6.59e-88 271
MS.gene03848.t1 AT2G05790 46.959 296 150 4 2 293 50 342 2.51e-87 270
MS.gene03848.t1 AT2G16230 45.085 295 161 1 2 295 50 344 3.94e-86 267
MS.gene03848.t1 AT5G24318 42.517 294 164 2 3 295 57 346 8.70e-85 259
MS.gene03848.t1 AT4G34480 45.085 295 161 1 2 295 51 345 3.28e-84 258
MS.gene03848.t1 AT5G24318 42.517 294 164 2 3 295 57 346 1.02e-83 259
MS.gene03848.t1 AT5G24318 42.517 294 164 2 3 295 57 346 1.54e-83 259
MS.gene03848.t1 AT5G42720 44.027 293 162 2 5 295 54 346 3.20e-83 258
MS.gene03848.t1 AT4G34480 44.118 306 170 1 2 306 51 356 2.97e-82 257
MS.gene03848.t1 AT5G56590 41.611 298 172 2 2 297 51 348 2.21e-80 253
MS.gene03848.t1 AT4G29360 43.289 298 167 2 1 297 51 347 3.82e-76 242
MS.gene03848.t1 AT4G29360 43.289 298 167 2 1 297 61 357 5.62e-76 242
MS.gene03848.t1 AT3G07320 39.735 302 176 5 1 297 49 349 3.24e-74 235
MS.gene03848.t1 AT2G01630 39.333 300 177 3 2 297 48 346 7.70e-72 227
MS.gene03848.t1 AT2G01630 39.333 300 177 3 2 297 48 346 1.91e-71 228
MS.gene03848.t1 AT2G39640 41.724 290 161 4 6 295 59 340 2.55e-71 230
MS.gene03848.t1 AT2G01630 39.333 300 177 3 2 297 48 346 1.39e-70 227
MS.gene03848.t1 AT4G14080 38.127 299 180 4 6 300 71 368 1.50e-69 224
MS.gene03848.t1 AT4G16260 39.384 292 169 4 2 293 20 303 6.82e-69 217
MS.gene03848.t1 AT4G16260 39.384 292 169 4 2 293 49 332 1.60e-68 217
MS.gene03848.t1 AT1G11820 38.333 300 180 3 2 297 69 367 2.27e-68 218
MS.gene03848.t1 AT4G34480 47.325 243 128 0 64 306 3 245 3.47e-68 218
MS.gene03848.t1 AT3G23770 39.130 299 174 5 6 297 66 363 3.95e-68 220
MS.gene03848.t1 AT3G55430 38.486 317 177 9 2 308 54 362 6.13e-68 219
MS.gene03848.t1 AT1G11820 38.462 299 179 3 3 297 70 367 2.91e-67 218
MS.gene03848.t1 AT1G11820 38.462 299 179 3 3 297 70 367 1.01e-66 217
MS.gene03848.t1 AT3G13560 36.177 293 182 3 3 291 52 343 5.21e-66 215
MS.gene03848.t1 AT3G13560 36.177 293 182 3 3 291 52 343 5.21e-66 215
MS.gene03848.t1 AT3G13560 36.177 293 182 3 3 291 52 343 5.21e-66 215
MS.gene03848.t1 AT3G13560 36.177 293 182 3 3 291 52 343 5.21e-66 215
MS.gene03848.t1 AT3G13560 36.177 293 182 3 3 291 52 343 5.21e-66 215
MS.gene03848.t1 AT3G57270 37.627 295 172 7 2 293 52 337 4.38e-64 206
MS.gene03848.t1 AT1G66250 36.700 297 182 4 3 295 58 352 5.04e-62 205
MS.gene03848.t1 AT3G46570 38.591 298 173 7 3 296 54 345 1.57e-60 197
MS.gene03848.t1 AT5G24318 45.024 211 111 2 86 295 1 207 9.05e-60 194
MS.gene03848.t1 AT3G61810 33.667 300 183 5 1 293 79 369 3.72e-55 184
MS.gene03848.t1 AT1G77790 35.593 295 179 7 2 293 55 341 8.85e-53 176
MS.gene03848.t1 AT3G57260 33.447 293 181 7 2 293 57 336 4.66e-51 172
MS.gene03848.t1 AT3G57260 33.447 293 181 7 2 293 73 352 6.64e-51 172
MS.gene03848.t1 AT1G77780 35.034 294 181 7 1 293 48 332 8.06e-51 172
MS.gene03848.t1 AT3G57240 34.021 291 177 8 2 291 60 336 5.62e-50 169
MS.gene03848.t1 AT3G55780 36.301 292 170 9 1 284 59 342 2.99e-48 166
MS.gene03848.t1 AT5G20340 34.797 296 181 9 1 293 67 353 7.60e-47 161
MS.gene03848.t1 AT5G20390 33.784 296 184 8 1 293 57 343 9.77e-47 160
MS.gene03848.t1 AT1G64760 33.770 305 185 11 2 297 50 346 2.95e-44 157
MS.gene03848.t1 AT1G64760 33.770 305 185 11 2 297 50 346 2.95e-44 157
MS.gene03848.t1 AT2G19440 34.098 305 184 11 2 297 46 342 6.78e-44 156
MS.gene03848.t1 AT2G19440 34.098 305 184 11 2 297 46 342 6.78e-44 156
MS.gene03848.t1 AT5G20870 34.688 320 181 14 5 307 58 366 1.00e-43 156
MS.gene03848.t1 AT5G20330 33.446 296 185 9 1 293 58 344 1.26e-43 152
MS.gene03848.t1 AT5G18220 30.159 315 207 9 2 310 55 362 2.05e-42 152
MS.gene03848.t1 AT5G58090 32.119 302 190 10 2 295 47 341 1.36e-41 150
MS.gene03848.t1 AT1G33220 31.864 295 181 8 1 293 58 334 3.56e-40 143
MS.gene03848.t1 AT3G04010 32.188 320 196 10 2 310 58 367 9.90e-40 145
MS.gene03848.t1 AT4G31140 30.565 301 194 10 3 295 53 346 5.67e-39 143
MS.gene03848.t1 AT3G24330 33.770 305 183 12 4 297 66 362 1.41e-37 140
MS.gene03848.t1 AT5G64790 28.803 309 205 10 3 303 54 355 1.73e-37 139
MS.gene03848.t1 AT5G58480 30.897 301 193 9 3 295 52 345 3.19e-37 138
MS.gene03848.t1 AT4G17180 29.801 302 190 11 3 295 51 339 7.01e-33 126
MS.gene03848.t1 AT5G20560 29.371 286 192 7 2 285 59 336 1.61e-30 118

Find 50 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGTTATGCAATTTATTT+TGG 0.148253 4.1:+44983618 None:intergenic
TCAAATGTTGAATTTGTTAT+AGG 0.179247 4.1:-44983730 MS.gene03848:CDS
ACCCTTCTAAAGCTCAAAAT+TGG 0.203490 4.1:-44983675 MS.gene03848:CDS
GTTGAATTTGTTATAGGAAT+TGG 0.226002 4.1:-44983724 MS.gene03848:CDS
AACAAATTCAACATTTGATT+TGG 0.238369 4.1:+44983735 None:intergenic
TAGTTTAAAGATACCTCATT+AGG 0.279200 4.1:+44983316 None:intergenic
GAGAATGCAAGTAGAACATT+TGG 0.330768 4.1:+44983757 None:intergenic
AATGGCGACACCGATGAAAT+TGG 0.356366 4.1:-44983136 MS.gene03848:CDS
TCATCAAGAAATTCAACAAT+TGG 0.359860 4.1:+44983394 None:intergenic
ATTGGAACCAATGGATTTCC+AGG 0.366015 4.1:+44983043 None:intergenic
TTATACCAATGTTGAAGTTA+AGG 0.367370 4.1:-44983182 MS.gene03848:CDS
TCATAATGTAAATTTGTGTT+TGG 0.368318 4.1:+44983259 None:intergenic
ATTTCAACCAAATGAAGGTT+TGG 0.371282 4.1:-44983287 MS.gene03848:CDS
GGATCAACCAAACCTTCATT+TGG 0.387383 4.1:+44983280 None:intergenic
TGTAATCCAATGTTATAAAC+TGG 0.399547 4.1:+44982929 None:intergenic
CCTGCTTCAGAAAGAAACTA+TGG 0.401079 4.1:-44982974 MS.gene03848:CDS
AGTTTAAAGATACCTCATTA+GGG 0.402444 4.1:+44983317 None:intergenic
TATGCTGCAATCAAAGTGAT+AGG 0.404460 4.1:-44983205 MS.gene03848:CDS
CATAATTCACTTGTTAAGCT+TGG 0.409029 4.1:-44983526 MS.gene03848:CDS
CCATAGTTTCTTTCTGAAGC+AGG 0.437326 4.1:+44982974 None:intergenic
GAAGTTAAGGTTTCTGAGAC+AGG 0.447516 4.1:-44983169 MS.gene03848:CDS
CTGTTGTAACAGTAATTACT+TGG 0.448483 4.1:+44983497 None:intergenic
AATTCTTACCCTCCTTCGTC+CGG 0.452125 4.1:-44983451 MS.gene03848:CDS
GAGGAAAGACATGGAACTCC+TGG 0.456953 4.1:-44983061 MS.gene03848:CDS
AAAATAAATTGCATAACTGT+TGG 0.468767 4.1:-44983616 MS.gene03848:CDS
CAAATCTGACCTAAAAGAAC+CGG 0.469027 4.1:+44983432 None:intergenic
ACAAGGTTATCTACCACAAA+TGG 0.472431 4.1:-44982909 MS.gene03848:CDS
GAGACAGGTTGGCCTTCAAA+TGG 0.474442 4.1:-44983154 MS.gene03848:CDS
GCATTATTTCAACCAAATGA+AGG 0.475383 4.1:-44983292 MS.gene03848:CDS
GGTAGACCAGTTTATAACAT+TGG 0.488178 4.1:-44982935 MS.gene03848:CDS
TTTCTTTCTGAAGCAGGACC+AGG 0.491860 4.1:+44982980 None:intergenic
AAATTTGCTTAGAAGAATTG+AGG 0.496626 4.1:-44983080 MS.gene03848:CDS
TTATAAACTGGTCTACCATC+AGG 0.497918 4.1:+44982941 None:intergenic
TTGGATGGCACAACCATTTG+TGG 0.503269 4.1:+44982896 None:intergenic
TCATCGGTGTCGCCATTTGA+AGG 0.506564 4.1:+44983142 None:intergenic
TATACATCAATTGGAACCAA+TGG 0.507282 4.1:+44983034 None:intergenic
GTTTATAACATTGGATTACA+AGG 0.510666 4.1:-44982926 MS.gene03848:CDS
TGGTGTAGCACCAATTTCAT+CGG 0.522068 4.1:+44983126 None:intergenic
AGAAACCTTAACTTCAACAT+TGG 0.523456 4.1:+44983177 None:intergenic
TTGCTTACATTAGATTTGGA+TGG 0.524160 4.1:+44982881 None:intergenic
TATGGACTTTATTATCCTGA+TGG 0.553442 4.1:-44982956 MS.gene03848:CDS
TTTAATGAAGATTTGAAACC+TGG 0.554891 4.1:-44982998 MS.gene03848:CDS
TGGAACTCCTGGAAATCCAT+TGG 0.557029 4.1:-44983050 MS.gene03848:CDS
TTAAGGTTTCTGAGACAGGT+TGG 0.560373 4.1:-44983165 MS.gene03848:CDS
TAAAAGAACCGGACGAAGGA+GGG 0.563985 4.1:+44983443 None:intergenic
CTAAAAGAACCGGACGAAGG+AGG 0.620046 4.1:+44983442 None:intergenic
ATATAAAGATAACCCTAATG+AGG 0.620782 4.1:-44983329 MS.gene03848:CDS
AGAAGAATTGAGGAAAGACA+TGG 0.624353 4.1:-44983070 MS.gene03848:CDS
CAAAATGCAAAATTGTACAA+TGG 0.632067 4.1:-44983103 MS.gene03848:CDS
GACCTAAAAGAACCGGACGA+AGG 0.662778 4.1:+44983439 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAAAAATTAATCTCAAA+AGG + chr4.1:44983705-44983724 None:intergenic 15.0%
!! TATCTTTATATGCAAAAAAA+GGG + chr4.1:44983211-44983230 None:intergenic 15.0%
!! TTATCTTTATATGCAAAAAA+AGG + chr4.1:44983212-44983231 None:intergenic 15.0%
!! AAAATAAATTGCATAACTGT+TGG - chr4.1:44982933-44982952 MS.gene03848:CDS 20.0%
!! AACAAATTCAACATTTGATT+TGG + chr4.1:44982817-44982836 None:intergenic 20.0%
!! TCATAATGTAAATTTGTGTT+TGG + chr4.1:44983293-44983312 None:intergenic 20.0%
!!! AACAGTTATGCAATTTATTT+TGG + chr4.1:44982934-44982953 None:intergenic 20.0%
!!! AATTTATTTTGGTTTGTGAA+AGG + chr4.1:44982923-44982942 None:intergenic 20.0%
!!! AATTTTGCTTACATTAGATT+TGG + chr4.1:44983675-44983694 None:intergenic 20.0%
!!! TCAAATGTTGAATTTGTTAT+AGG - chr4.1:44982819-44982838 MS.gene03848:intron 20.0%
! AGTTTAAAGATACCTCATTA+GGG + chr4.1:44983235-44983254 None:intergenic 25.0%
! ATATAAAGATAACCCTAATG+AGG - chr4.1:44983220-44983239 MS.gene03848:CDS 25.0%
! CAAAATGCAAAATTGTACAA+TGG - chr4.1:44983446-44983465 MS.gene03848:CDS 25.0%
! GCAAAAACATATACATCAAT+TGG + chr4.1:44983527-44983546 None:intergenic 25.0%
! GTTTATAACATTGGATTACA+AGG - chr4.1:44983623-44983642 MS.gene03848:CDS 25.0%
! TAGTTTAAAGATACCTCATT+AGG + chr4.1:44983236-44983255 None:intergenic 25.0%
! TCATCAAGAAATTCAACAAT+TGG + chr4.1:44983158-44983177 None:intergenic 25.0%
! TGTAATCCAATGTTATAAAC+TGG + chr4.1:44983623-44983642 None:intergenic 25.0%
! TTATACCAATGTTGAAGTTA+AGG - chr4.1:44983367-44983386 MS.gene03848:CDS 25.0%
!! AAATTTGCTTAGAAGAATTG+AGG - chr4.1:44983469-44983488 MS.gene03848:CDS 25.0%
!! AGCTCATTCTTTTAACATTT+TGG - chr4.1:44983073-44983092 MS.gene03848:CDS 25.0%
!! GATTAATTTTTATGTACCGA+CGG - chr4.1:44983710-44983729 MS.gene03848:CDS 25.0%
!! TTATGAACATTTTGCATTGA+AGG + chr4.1:44983008-44983027 None:intergenic 25.0%
!! TTGTACAATTTTGCATTTTG+TGG + chr4.1:44983446-44983465 None:intergenic 25.0%
!! TTTAATGAAGATTTGAAACC+TGG - chr4.1:44983551-44983570 MS.gene03848:CDS 25.0%
!!! GTTGAATTTGTTATAGGAAT+TGG - chr4.1:44982825-44982844 MS.gene03848:intron 25.0%
AGAAACCTTAACTTCAACAT+TGG + chr4.1:44983375-44983394 None:intergenic 30.0%
ATTTCAACCAAATGAAGGTT+TGG - chr4.1:44983262-44983281 MS.gene03848:CDS 30.0%
CTGTTGTAACAGTAATTACT+TGG + chr4.1:44983055-44983074 None:intergenic 30.0%
GCATTATTTCAACCAAATGA+AGG - chr4.1:44983257-44983276 MS.gene03848:CDS 30.0%
TATACATCAATTGGAACCAA+TGG + chr4.1:44983518-44983537 None:intergenic 30.0%
TATGGACTTTATTATCCTGA+TGG - chr4.1:44983593-44983612 MS.gene03848:CDS 30.0%
TTGCTTACATTAGATTTGGA+TGG + chr4.1:44983671-44983690 None:intergenic 30.0%
! CATAATTCACTTGTTAAGCT+TGG - chr4.1:44983023-44983042 MS.gene03848:CDS 30.0%
!!! ATCCAATTTTGAGCTTTAGA+AGG + chr4.1:44982879-44982898 None:intergenic 30.0%
!!! ATTTTGGTTTGTGAAAGGTA+AGG + chr4.1:44982918-44982937 None:intergenic 30.0%
!!! TCCAATTTTGAGCTTTAGAA+GGG + chr4.1:44982878-44982897 None:intergenic 30.0%
ACAAGGTTATCTACCACAAA+TGG - chr4.1:44983640-44983659 MS.gene03848:CDS 35.0%
AGAAGAATTGAGGAAAGACA+TGG - chr4.1:44983479-44983498 MS.gene03848:CDS 35.0%
CAAATCTGACCTAAAAGAAC+CGG + chr4.1:44983120-44983139 None:intergenic 35.0%
GAGAATGCAAGTAGAACATT+TGG + chr4.1:44982795-44982814 None:intergenic 35.0%
GGTAGACCAGTTTATAACAT+TGG - chr4.1:44983614-44983633 MS.gene03848:CDS 35.0%
TATGCTGCAATCAAAGTGAT+AGG - chr4.1:44983344-44983363 MS.gene03848:CDS 35.0%
! TTATAAACTGGTCTACCATC+AGG + chr4.1:44983611-44983630 None:intergenic 35.0%
!! ACCCTTCTAAAGCTCAAAAT+TGG - chr4.1:44982874-44982893 MS.gene03848:intron 35.0%
ACCTGCATCAAATCACATCT+CGG + chr4.1:44983792-44983811 None:intergenic 40.0%
CCTGCTTCAGAAAGAAACTA+TGG - chr4.1:44983575-44983594 MS.gene03848:CDS 40.0%
GGATCAACCAAACCTTCATT+TGG + chr4.1:44983272-44983291 None:intergenic 40.0%
TGGTGTAGCACCAATTTCAT+CGG + chr4.1:44983426-44983445 None:intergenic 40.0%
TTAAAAAACGTGCACACCGT+CGG + chr4.1:44983729-44983748 None:intergenic 40.0%
! ATTGGAACCAATGGATTTCC+AGG + chr4.1:44983509-44983528 None:intergenic 40.0%
!! CCATAGTTTCTTTCTGAAGC+AGG + chr4.1:44983578-44983597 None:intergenic 40.0%
!! GAAGTTAAGGTTTCTGAGAC+AGG - chr4.1:44983380-44983399 MS.gene03848:CDS 40.0%
!! TTAAGGTTTCTGAGACAGGT+TGG - chr4.1:44983384-44983403 MS.gene03848:CDS 40.0%
AATGGCGACACCGATGAAAT+TGG - chr4.1:44983413-44983432 MS.gene03848:CDS 45.0%
AATTCTTACCCTCCTTCGTC+CGG - chr4.1:44983098-44983117 MS.gene03848:CDS 45.0%
TAAAAGAACCGGACGAAGGA+GGG + chr4.1:44983109-44983128 None:intergenic 45.0%
TCCGAGATGTGATTTGATGC+AGG - chr4.1:44983788-44983807 MS.gene03848:CDS 45.0%
TGGAACTCCTGGAAATCCAT+TGG - chr4.1:44983499-44983518 MS.gene03848:CDS 45.0%
TTGGATGGCACAACCATTTG+TGG + chr4.1:44983656-44983675 None:intergenic 45.0%
!! TTTCTTTCTGAAGCAGGACC+AGG + chr4.1:44983572-44983591 None:intergenic 45.0%
CTAAAAGAACCGGACGAAGG+AGG + chr4.1:44983110-44983129 None:intergenic 50.0%
GACCTAAAAGAACCGGACGA+AGG + chr4.1:44983113-44983132 None:intergenic 50.0%
GAGGAAAGACATGGAACTCC+TGG - chr4.1:44983488-44983507 MS.gene03848:CDS 50.0%
! CTCCTTCGTCCGGTTCTTTT+AGG - chr4.1:44983108-44983127 MS.gene03848:CDS 50.0%
!! GAGACAGGTTGGCCTTCAAA+TGG - chr4.1:44983395-44983414 MS.gene03848:CDS 50.0%
!! TCATCGGTGTCGCCATTTGA+AGG + chr4.1:44983410-44983429 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 44982756 44983815 44982756 ID=MS.gene03848
chr4.1 mRNA 44982756 44983815 44982756 ID=MS.gene03848.t1;Parent=MS.gene03848
chr4.1 exon 44982889 44983815 44982889 ID=MS.gene03848.t1.exon1;Parent=MS.gene03848.t1
chr4.1 CDS 44982889 44983815 44982889 ID=cds.MS.gene03848.t1;Parent=MS.gene03848.t1
chr4.1 exon 44982756 44982761 44982756 ID=MS.gene03848.t1.exon2;Parent=MS.gene03848.t1
chr4.1 CDS 44982756 44982761 44982756 ID=cds.MS.gene03848.t1;Parent=MS.gene03848.t1
Gene Sequence

>MS.gene03848

ATGAATGTGACAAGAATCAAACTCTATGATGCTGATCCAAATGTTCTACTTGCATTCTCCAAATCAAATGTTGAATTTGTTATAGGAATTGGAAATGAGCATTTACAAAATATGACAAACCCTTCTAAAGCTCAAAATTGGATTCAACATCATGTTCTACCTTACCTTTCACAAACCAAAATAAATTGCATAACTGTTGGAAATGAAGTTTTCAATAGCAATGATACTCAACTAATGTTGAATCTTCTACCTTCAATGCAAAATGTTCATAATTCACTTGTTAAGCTTGGATTAGACCAAGTAATTACTGTTACAACAGCTCATTCTTTTAACATTTTGGATAATTCTTACCCTCCTTCGTCCGGTTCTTTTAGGTCAGATTTGATTCAATATATTCAACCAATTGTTGAATTTCTTGATGAAATTAAATCATCTTTTCATATTAATGCTTACCCTTTTTTTGCATATAAAGATAACCCTAATGAGGTATCTTTAAACTATGCATTATTTCAACCAAATGAAGGTTTGGTTGATCCAAACACAAATTTACATTATGATAACATGTTGTATGCTCAAATTGATGCTGTTTATGCTGCAATCAAAGTGATAGGTTATACCAATGTTGAAGTTAAGGTTTCTGAGACAGGTTGGCCTTCAAATGGCGACACCGATGAAATTGGTGCTACACCACAAAATGCAAAATTGTACAATGGAAATTTGCTTAGAAGAATTGAGGAAAGACATGGAACTCCTGGAAATCCATTGGTTCCAATTGATGTATATGTTTTTGCACTTTTTAATGAAGATTTGAAACCTGGTCCTGCTTCAGAAAGAAACTATGGACTTTATTATCCTGATGGTAGACCAGTTTATAACATTGGATTACAAGGTTATCTACCACAAATGGTTGTGCCATCCAAATCTAATGTAAGCAAAATTCTATTTACCTTTTGAGATTAATTTTTATGTACCGACGGTGTGCACGTTTTTTAAACGAATGATGTCACATTGTTATGTTATTCTTAAAACATCTCCGAGATGTGATTTGATGCAGGTGTAA
Protein sequence

>MS.gene03848.t1

MNVTRIKLYDADPNVLLAFSKSNVEFVIGIGNEHLQNMTNPSKAQNWIQHHVLPYLSQTKINCITVGNEVFNSNDTQLMLNLLPSMQNVHNSLVKLGLDQVITVTTAHSFNILDNSYPPSSGSFRSDLIQYIQPIVEFLDEIKSSFHINAYPFFAYKDNPNEVSLNYALFQPNEGLVDPNTNLHYDNMLYAQIDAVYAAIKVIGYTNVEVKVSETGWPSNGDTDEIGATPQNAKLYNGNLLRRIEERHGTPGNPLVPIDVYVFALFNEDLKPGPASERNYGLYYPDGRPVYNIGLQGYLPQMVVPSKSNV