Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039747.t1 | XP_024635560.1 | 99 | 508 | 5 | 0 | 1 | 508 | 9 | 516 | 2.90E-294 | 1020.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039747.t1 | Q9M367 | 74.5 | 513 | 124 | 2 | 1 | 506 | 1 | 513 | 9.7e-222 | 770.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039747.t1 | A0A396IM07 | 99.0 | 508 | 5 | 0 | 1 | 508 | 9 | 516 | 2.1e-294 | 1020.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene063319 | MS.gene039747 | PPI |
| MS.gene66413 | MS.gene039747 | PPI |
| MS.gene059541 | MS.gene039747 | PPI |
| MS.gene66405 | MS.gene039747 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039747.t1 | MTR_3g018770 | 98.821 | 509 | 5 | 1 | 1 | 508 | 1 | 509 | 0.0 | 1055 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039747.t1 | AT3G61710 | 74.464 | 513 | 124 | 2 | 1 | 506 | 1 | 513 | 0.0 | 779 |
| MS.gene039747.t1 | AT3G61710 | 74.600 | 437 | 104 | 2 | 1 | 430 | 1 | 437 | 0.0 | 664 |
| MS.gene039747.t1 | AT3G61710 | 73.901 | 364 | 88 | 2 | 1 | 357 | 1 | 364 | 0.0 | 538 |
| MS.gene039747.t1 | AT3G61710 | 77.365 | 296 | 60 | 2 | 69 | 357 | 1 | 296 | 1.24e-155 | 446 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGCATCTTACTGGAATTT+TGG | 0.123213 | 3.3:-11382988 | None:intergenic |
| AATATGTTATGTTCAGTTTC+TGG | 0.132096 | 3.3:-11385738 | None:intergenic |
| TGTAATAATGCTTACTTATA+AGG | 0.156640 | 3.3:-11385180 | None:intergenic |
| GGAGGAGAGAGATGCAATTT+TGG | 0.238592 | 3.3:+11382839 | MS.gene039747:intron |
| GACTCTTCCATTGTCTTTCC+AGG | 0.250506 | 3.3:-11380538 | None:intergenic |
| ATTTCTCATGATGGAGAGTT+TGG | 0.268411 | 3.3:+11382933 | MS.gene039747:CDS |
| GCTTTAATAAGCAGGAAAAT+TGG | 0.278141 | 3.3:+11385552 | MS.gene039747:CDS |
| GATGAGAGAGACGACGAAAA+AGG | 0.286671 | 3.3:+11378744 | None:intergenic |
| ATCTAGCCGCTTCAAAGAAT+TGG | 0.303262 | 3.3:+11382385 | MS.gene039747:CDS |
| TTTCCTGCTTATTAAAGCTT+TGG | 0.306193 | 3.3:-11385547 | None:intergenic |
| ATTTGAAATGGGCACTCTAT+TGG | 0.321192 | 3.3:+11385603 | MS.gene039747:CDS |
| TGTGATGAGCACAACTAAAA+TGG | 0.323097 | 3.3:+11380435 | MS.gene039747:CDS |
| TTGGTCCAGTCAACTTGTTT+TGG | 0.324830 | 3.3:+11385040 | MS.gene039747:CDS |
| TTCCTGCTTATTAAAGCTTT+GGG | 0.325626 | 3.3:-11385546 | None:intergenic |
| AACTCTCCATCATGAGAAAT+AGG | 0.329432 | 3.3:-11382930 | None:intergenic |
| TCAGTTTCTGGATTCAGATT+TGG | 0.333866 | 3.3:-11385726 | None:intergenic |
| ACACACTCTGCAATTTGAAA+TGG | 0.335752 | 3.3:+11385591 | MS.gene039747:CDS |
| TTCAACGATCCTTCTCGTTC+TGG | 0.342444 | 3.3:+11378874 | MS.gene039747:CDS |
| TGGGCACTCTATTGGTTTGT+TGG | 0.352054 | 3.3:+11385611 | MS.gene039747:CDS |
| TGAGCCTGAGGTCTTTGCTT+TGG | 0.359710 | 3.3:-11380478 | None:intergenic |
| TTTGAAACATTTAGGTAGAA+TGG | 0.363731 | 3.3:+11383806 | MS.gene039747:intron |
| GAAACTTCGGTCGGAAATAC+TGG | 0.365381 | 3.3:-11383882 | None:intergenic |
| TATGTTGTTCTCTTGATCCT+TGG | 0.366586 | 3.3:-11385133 | None:intergenic |
| TGGCTTCAGTACCACGCTTT+GGG | 0.368320 | 3.3:-11385706 | None:intergenic |
| GTTTCAATTGATTTCACATC+AGG | 0.393088 | 3.3:+11382642 | MS.gene039747:CDS |
| TTCCATTGTCTTTCCAGGAT+GGG | 0.403861 | 3.3:-11380533 | None:intergenic |
| AGCCTCGCTGAGGACATCTT+TGG | 0.404226 | 3.3:-11381022 | None:intergenic |
| TTGGCTTCAGTACCACGCTT+TGG | 0.410296 | 3.3:-11385707 | None:intergenic |
| GTTCTCTTGATCCTTGGACT+TGG | 0.416693 | 3.3:-11385127 | None:intergenic |
| TTGAAACATTTAGGTAGAAT+GGG | 0.417297 | 3.3:+11383807 | MS.gene039747:intron |
| CGCATGCAGTCAAGGCATAA+AGG | 0.417474 | 3.3:-11380907 | None:intergenic |
| CTTCCATTGTCTTTCCAGGA+TGG | 0.419499 | 3.3:-11380534 | None:intergenic |
| CTTATTAAAGCTTTGGGTGA+TGG | 0.421070 | 3.3:-11385540 | None:intergenic |
| GGTGGAAACTCGTTGTCCCC+GGG | 0.421657 | 3.3:+11380604 | MS.gene039747:CDS |
| TGCTTGTGAAACTTCAATCT+TGG | 0.428703 | 3.3:-11382862 | None:intergenic |
| TGGTGGAAACTCGTTGTCCC+CGG | 0.429279 | 3.3:+11380603 | MS.gene039747:CDS |
| GTCTGATAAGCTTGATAAAG+AGG | 0.439310 | 3.3:+11380936 | MS.gene039747:CDS |
| CGCAATCCACTTTGCATCGT+CGG | 0.445068 | 3.3:+11378826 | MS.gene039747:CDS |
| GGGTCGGAATCGGAGCTTAC+CGG | 0.447487 | 3.3:+11378765 | MS.gene039747:CDS |
| CACACTCTGCAATTTGAAAT+GGG | 0.449196 | 3.3:+11385592 | MS.gene039747:CDS |
| CAGCTCATAAGTACTGTTGC+TGG | 0.456802 | 3.3:-11384626 | None:intergenic |
| GCAGTTCTGACAAACCCATC+GGG | 0.459373 | 3.3:-11378803 | None:intergenic |
| CACCCAAAGCTTTAATAAGC+AGG | 0.463428 | 3.3:+11385544 | MS.gene039747:CDS |
| CACACTGCTAGCACCATGAA+TGG | 0.472744 | 3.3:-11380416 | None:intergenic |
| GGTAACTTGAAACATTTCTC+TGG | 0.475394 | 3.3:-11385159 | None:intergenic |
| CAATCTCAAATGCTCGAGTC+AGG | 0.481760 | 3.3:-11380687 | None:intergenic |
| TGAAGCCTGTCTTAAACGCT+TGG | 0.485526 | 3.3:+11380990 | MS.gene039747:CDS |
| CGCAGAATTTGCCAAGTCCA+AGG | 0.489127 | 3.3:+11385116 | MS.gene039747:CDS |
| TTGCTGGTGTCTGTGATTCG+AGG | 0.493541 | 3.3:-11384610 | None:intergenic |
| CCCCTCCAAGCGTTTAAGAC+AGG | 0.494529 | 3.3:-11380995 | None:intergenic |
| ATACCATTGCTGAAAGAGGC+TGG | 0.496868 | 3.3:-11385646 | None:intergenic |
| TATGCAGAAGAAGGCAAGCT+TGG | 0.514890 | 3.3:-11383852 | None:intergenic |
| GTGCTCCAAAACAAGTTGAC+TGG | 0.516855 | 3.3:-11385045 | None:intergenic |
| CATTTGCCACCTCATAACTC+TGG | 0.520812 | 3.3:+11380643 | MS.gene039747:CDS |
| CGTGGTGATACATCCCATCC+TGG | 0.530828 | 3.3:+11380520 | MS.gene039747:CDS |
| AAGTCGGAATCTGCGTCTGA+TGG | 0.536084 | 3.3:+11380577 | MS.gene039747:CDS |
| TAGCCGCTTCAAAGAATTGG+AGG | 0.541238 | 3.3:+11382388 | MS.gene039747:CDS |
| AGCCTGTCTTAAACGCTTGG+AGG | 0.542097 | 3.3:+11380993 | MS.gene039747:CDS |
| ATGCAAGGTTCATCCATTCA+TGG | 0.544986 | 3.3:+11380403 | MS.gene039747:CDS |
| GCCTGTCTTAAACGCTTGGA+GGG | 0.547231 | 3.3:+11380994 | MS.gene039747:CDS |
| GGATGAGATAAATGCTGCAT+GGG | 0.551698 | 3.3:+11383828 | MS.gene039747:CDS |
| AGAAGAAAGAAGACTAGAAG+CGG | 0.552430 | 3.3:+11382298 | MS.gene039747:CDS |
| TCTGCGTCTGATGGAGGTGG+TGG | 0.554403 | 3.3:+11380586 | MS.gene039747:CDS |
| CTTCCAGCCTCTTTCAGCAA+TGG | 0.561642 | 3.3:+11385643 | MS.gene039747:CDS |
| GGCACATTGTATGCAGAAGA+AGG | 0.565635 | 3.3:-11383861 | None:intergenic |
| CTGTCTTAAACGCTTGGAGG+GGG | 0.566042 | 3.3:+11380996 | MS.gene039747:CDS |
| GATGTATCACCACGAGGCCG+AGG | 0.567728 | 3.3:-11380511 | None:intergenic |
| GGCAGTTCTGACAAACCCAT+CGG | 0.568521 | 3.3:-11378804 | None:intergenic |
| TCGACGCCGACGATGCAAAG+TGG | 0.572876 | 3.3:-11378832 | None:intergenic |
| AAGCAAAGACCTCAGGCTCA+AGG | 0.574216 | 3.3:+11380481 | MS.gene039747:CDS |
| GCTCCTCCAATTCTTTGAAG+CGG | 0.574855 | 3.3:-11382391 | None:intergenic |
| GTTGCCAAAGCAAAGACCTC+AGG | 0.579073 | 3.3:+11380474 | MS.gene039747:CDS |
| TGACCACCATGATCAACCCC+CGG | 0.580351 | 3.3:-11380622 | None:intergenic |
| AGGCTCAAGGAGTTCCTCCT+CGG | 0.586199 | 3.3:+11380494 | MS.gene039747:CDS |
| TGCTGGTGTCTGTGATTCGA+GGG | 0.588115 | 3.3:-11384609 | None:intergenic |
| GTGGAAACTCGTTGTCCCCG+GGG | 0.589060 | 3.3:+11380605 | MS.gene039747:CDS |
| GAATTGAATCCAGAGTTATG+AGG | 0.590276 | 3.3:-11380652 | None:intergenic |
| ATCCCATCCTGGAAAGACAA+TGG | 0.590592 | 3.3:+11380531 | MS.gene039747:CDS |
| GGGTACAAAGATCCTACAGC+TGG | 0.595630 | 3.3:-11385686 | None:intergenic |
| CCTGTCTTAAACGCTTGGAG+GGG | 0.597661 | 3.3:+11380995 | MS.gene039747:CDS |
| GGAGGAACTCCTTGAGCCTG+AGG | 0.598305 | 3.3:-11380490 | None:intergenic |
| CATGCTGAAGTGCCAGCTGT+AGG | 0.598389 | 3.3:+11385674 | MS.gene039747:CDS |
| TCGGAATCTGCGTCTGATGG+AGG | 0.599215 | 3.3:+11380580 | MS.gene039747:CDS |
| GCGAACCTGTGTCTGTGTTG+TGG | 0.599926 | 3.3:-11380710 | None:intergenic |
| ACAAAACTCGCATGCAGTCA+AGG | 0.604059 | 3.3:-11380915 | None:intergenic |
| AACTTGAAACATTTCTCTGG+TGG | 0.604430 | 3.3:-11385156 | None:intergenic |
| GGAGTTCCTCCTCGGCCTCG+TGG | 0.607193 | 3.3:+11380502 | MS.gene039747:CDS |
| GAATCTGCGTCTGATGGAGG+TGG | 0.607751 | 3.3:+11380583 | MS.gene039747:CDS |
| TGAAGTAAATGCTGAACTGA+AGG | 0.613224 | 3.3:+11382349 | MS.gene039747:CDS |
| GATTGCCACAACACAGACAC+AGG | 0.618152 | 3.3:+11380705 | MS.gene039747:CDS |
| AGGATCGTTGAAGTACTTGT+CGG | 0.618370 | 3.3:-11378863 | None:intergenic |
| ATTCGAGGGTAGCTACCCAT+AGG | 0.618428 | 3.3:-11384595 | None:intergenic |
| AAGGATACTGAAACTTCGGT+CGG | 0.619914 | 3.3:-11383891 | None:intergenic |
| GGGATGAGATAAATGCTGCA+TGG | 0.624474 | 3.3:+11383827 | MS.gene039747:CDS |
| GAAGATACCATTGCTGAAAG+AGG | 0.628303 | 3.3:-11385650 | None:intergenic |
| TAAATGTGTGTAGGGATGCA+AGG | 0.633755 | 3.3:+11380388 | MS.gene039747:intron |
| CAAATTCTGCGAAATCCTTG+AGG | 0.638626 | 3.3:-11385104 | None:intergenic |
| CGACGATGCAAAGTGGATTG+CGG | 0.640313 | 3.3:-11378825 | None:intergenic |
| AGAAACTAACCAGAACGAGA+AGG | 0.653735 | 3.3:-11378883 | None:intergenic |
| GTATCACCACGAGGCCGAGG+AGG | 0.654136 | 3.3:-11380508 | None:intergenic |
| TCGGAATCGGAGCTTACCGG+TGG | 0.658622 | 3.3:+11378768 | MS.gene039747:CDS |
| TTGAATCCAGAGTTATGAGG+TGG | 0.665081 | 3.3:-11380649 | None:intergenic |
| CAGTTCTGACAAACCCATCG+GGG | 0.678384 | 3.3:-11378802 | None:intergenic |
| AGTTCTGACAAACCCATCGG+GGG | 0.683361 | 3.3:-11378801 | None:intergenic |
| TAAGAAAATCAGCCTCGCTG+AGG | 0.688641 | 3.3:-11381032 | None:intergenic |
| GGATCTTTGTACCCAAAGCG+TGG | 0.690117 | 3.3:+11385695 | MS.gene039747:CDS |
| GGCCAAAGATGTCCTCAGCG+AGG | 0.702573 | 3.3:+11381020 | MS.gene039747:CDS |
| GCTTCAAAGAATTGGAGGAG+CGG | 0.703613 | 3.3:+11382393 | MS.gene039747:CDS |
| TGGAAACTCGTTGTCCCCGG+GGG | 0.705053 | 3.3:+11380606 | MS.gene039747:CDS |
| GATGAGATAAATGCTGCATG+GGG | 0.762402 | 3.3:+11383829 | MS.gene039747:CDS |
| GGATGGGATGTATCACCACG+AGG | 0.784660 | 3.3:-11380517 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 11378745 | 11385748 | 11378745 | ID=MS.gene039747 |
| chr3.3 | mRNA | 11378745 | 11385748 | 11378745 | ID=MS.gene039747.t1;Parent=MS.gene039747 |
| chr3.3 | exon | 11378745 | 11378895 | 11378745 | ID=MS.gene039747.t1.exon1;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11378745 | 11378895 | 11378745 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11380401 | 11380726 | 11380401 | ID=MS.gene039747.t1.exon2;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11380401 | 11380726 | 11380401 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11380898 | 11381068 | 11380898 | ID=MS.gene039747.t1.exon3;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11380898 | 11381068 | 11380898 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11382290 | 11382414 | 11382290 | ID=MS.gene039747.t1.exon4;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11382290 | 11382414 | 11382290 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11382615 | 11382663 | 11382615 | ID=MS.gene039747.t1.exon5;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11382615 | 11382663 | 11382615 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11382840 | 11382998 | 11382840 | ID=MS.gene039747.t1.exon6;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11382840 | 11382998 | 11382840 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11383820 | 11383905 | 11383820 | ID=MS.gene039747.t1.exon7;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11383820 | 11383905 | 11383820 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11384576 | 11384647 | 11384576 | ID=MS.gene039747.t1.exon8;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11384576 | 11384647 | 11384576 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11385038 | 11385187 | 11385038 | ID=MS.gene039747.t1.exon9;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11385038 | 11385187 | 11385038 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| chr3.3 | exon | 11385511 | 11385748 | 11385511 | ID=MS.gene039747.t1.exon10;Parent=MS.gene039747.t1 |
| chr3.3 | CDS | 11385511 | 11385748 | 11385511 | ID=cds.MS.gene039747.t1;Parent=MS.gene039747.t1 |
| Gene Sequence |
| Protein sequence |
