AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03986

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03986.t1 MTR_3g463960 90.511 274 25 1 1 274 1 273 2.21e-178 496
MS.gene03986.t1 MTR_3g463950 90.511 274 25 1 1 274 1 273 2.21e-178 496
MS.gene03986.t1 MTR_3g463970 90.511 274 25 1 1 274 1 273 2.21e-178 496
MS.gene03986.t1 MTR_3g463940 90.511 274 25 1 1 274 1 273 2.21e-178 496
MS.gene03986.t1 MTR_3g463930 85.765 281 32 2 1 274 1 280 2.03e-168 471
MS.gene03986.t1 MTR_3g448360 64.103 273 82 3 2 274 1 257 3.63e-120 348
MS.gene03986.t1 MTR_3g069520 62.731 271 83 4 4 274 3 255 1.73e-112 329
MS.gene03986.t1 MTR_3g438030 61.905 273 88 3 2 274 1 257 8.33e-112 327
MS.gene03986.t1 MTR_3g069780 61.905 273 88 3 2 274 1 257 1.69e-111 326
MS.gene03986.t1 MTR_2g079090 61.624 271 88 4 4 274 3 257 3.30e-111 325
MS.gene03986.t1 MTR_2g079080 61.624 271 88 4 4 274 3 257 6.77e-111 324
MS.gene03986.t1 MTR_0029s0050 62.313 268 85 4 7 274 6 257 1.12e-110 324
MS.gene03986.t1 MTR_0029s0100 61.940 268 86 4 7 274 6 257 7.38e-110 322
MS.gene03986.t1 MTR_2g043980 54.945 273 102 6 4 274 9 262 8.04e-93 278
MS.gene03986.t1 MTR_2g015650 48.708 271 123 3 6 274 6 262 1.44e-78 243
MS.gene03986.t1 MTR_2g043990 49.451 273 95 7 4 274 71 302 6.02e-77 237
MS.gene03986.t1 MTR_2g015580 47.083 240 117 5 42 274 45 281 3.14e-65 209
MS.gene03986.t1 MTR_2g015590 45.339 236 125 3 42 274 29 263 3.22e-63 202
MS.gene03986.t1 MTR_2g015590 45.339 236 125 3 42 274 29 263 1.97e-62 201
MS.gene03986.t1 MTR_2g015670 46.025 239 106 5 41 274 28 248 6.44e-62 199
MS.gene03986.t1 MTR_2g015630 45.923 233 117 5 49 274 36 266 1.10e-61 199
MS.gene03986.t1 MTR_3g466190 56.886 167 50 5 72 238 7 151 5.67e-52 169
MS.gene03986.t1 MTR_3g466240 46.497 157 55 1 118 274 2 129 6.20e-39 135
MS.gene03986.t1 MTR_4g104240 36.929 241 138 8 39 274 34 265 2.34e-38 138
MS.gene03986.t1 MTR_1g030220 33.451 284 152 11 4 274 8 267 5.36e-29 114
MS.gene03986.t1 MTR_7g087190 32.841 271 136 11 28 274 25 273 1.05e-28 113
MS.gene03986.t1 MTR_8g028160 34.182 275 143 14 19 274 17 272 6.47e-28 110
MS.gene03986.t1 MTR_7g116870 31.933 238 146 6 42 274 31 257 1.26e-27 110
MS.gene03986.t1 MTR_1g030340 32.872 289 141 11 2 274 19 270 1.61e-27 109
MS.gene03986.t1 MTR_7g033165 32.558 258 145 8 31 274 22 264 3.89e-27 107
MS.gene03986.t1 MTR_6g007090 35.270 241 137 8 42 274 37 266 4.11e-27 108
MS.gene03986.t1 MTR_1g082390 34.568 243 146 7 37 274 22 256 6.94e-27 107
MS.gene03986.t1 MTR_7g033165 32.558 258 145 8 31 274 22 264 1.09e-26 107
MS.gene03986.t1 MTR_1g030268 33.333 249 131 10 42 274 38 267 1.59e-26 106
MS.gene03986.t1 MTR_1g030268 33.333 249 131 10 42 274 38 267 3.83e-26 105
MS.gene03986.t1 MTR_1g030230 33.878 245 131 10 44 274 32 259 9.94e-26 104
MS.gene03986.t1 MTR_1g030275 34.538 249 128 11 42 274 37 266 1.78e-25 104
MS.gene03986.t1 MTR_7g033150 33.984 256 138 10 37 274 28 270 2.30e-25 103
MS.gene03986.t1 MTR_7g113860 33.730 252 130 11 40 274 25 256 7.90e-25 102
MS.gene03986.t1 MTR_8g089805 32.296 257 135 9 37 274 33 269 2.05e-24 101
MS.gene03986.t1 MTR_7g111780 31.535 241 131 10 45 274 37 254 7.56e-24 99.4
MS.gene03986.t1 MTR_7g113860 31.818 264 143 12 29 274 18 262 1.14e-23 99.4
MS.gene03986.t1 MTR_6g007095 31.802 283 164 10 1 274 6 268 3.75e-23 99.0
MS.gene03986.t1 MTR_7g033155 31.387 274 146 11 31 274 23 284 1.21e-21 93.6
MS.gene03986.t1 MTR_8g038680 32.099 243 146 9 42 274 26 259 3.30e-20 89.4
MS.gene03986.t1 MTR_8g012540 32.099 243 146 8 42 274 26 259 7.88e-20 88.2
MS.gene03986.t1 MTR_8g031430 31.250 272 153 14 8 274 10 252 4.16e-19 86.3
MS.gene03986.t1 MTR_8g031420 31.535 241 140 10 40 274 29 250 1.34e-18 84.7
MS.gene03986.t1 MTR_8g022810 29.752 242 140 9 43 274 40 261 2.03e-18 84.3
MS.gene03986.t1 MTR_8g031400 34.634 205 114 8 40 238 31 221 2.97e-18 84.0
MS.gene03986.t1 MTR_8g022810 30.357 224 130 8 43 256 40 247 3.77e-18 83.2
MS.gene03986.t1 MTR_8g031390 31.405 242 138 10 40 274 31 251 4.96e-18 82.8
MS.gene03986.t1 MTR_8g031390 31.405 242 138 10 40 274 31 251 6.86e-18 82.8
MS.gene03986.t1 MTR_2g017965 31.336 217 121 9 44 245 33 236 4.01e-17 80.5
MS.gene03986.t1 MTR_2g017955 30.732 205 122 7 44 238 33 227 4.38e-16 77.8
MS.gene03986.t1 MTR_3g098270 31.132 212 119 8 42 238 32 231 9.07e-16 76.6
MS.gene03986.t1 MTR_3g005650 31.280 211 130 7 44 248 30 231 1.57e-15 75.9
MS.gene03986.t1 MTR_2g044100 26.721 247 154 10 37 274 36 264 2.71e-15 75.5
MS.gene03986.t1 MTR_0009s0250 29.208 202 121 7 44 238 33 219 2.76e-15 75.1
MS.gene03986.t1 MTR_3g052580 29.630 243 142 8 44 274 28 253 3.13e-15 75.1
MS.gene03986.t1 MTR_3g052580 29.630 243 142 8 44 274 27 252 4.53e-15 74.7
MS.gene03986.t1 MTR_2g017925 29.048 210 129 7 44 243 33 232 4.74e-15 74.7
MS.gene03986.t1 MTR_2g033930 27.083 240 149 9 44 274 32 254 1.51e-14 73.2
MS.gene03986.t1 MTR_4g056280 26.786 280 159 10 9 274 6 253 1.96e-14 72.8
MS.gene03986.t1 MTR_3g052480 29.808 208 125 6 44 238 33 232 2.00e-14 72.8
MS.gene03986.t1 MTR_1g025780 28.992 238 147 9 44 274 41 263 2.09e-14 72.8
MS.gene03986.t1 MTR_2g017915 29.082 196 125 5 44 232 33 221 2.40e-14 72.4
MS.gene03986.t1 MTR_6g007100 30.081 246 139 9 42 274 31 256 3.10e-14 72.0
MS.gene03986.t1 MTR_4g035353 27.488 211 133 6 38 238 25 225 7.04e-14 71.2
MS.gene03986.t1 MTR_3g021460 27.311 238 152 7 28 255 16 242 9.88e-14 70.5
MS.gene03986.t1 MTR_3g052490 28.846 208 127 6 44 238 32 231 1.36e-13 70.5
MS.gene03986.t1 MTR_2g033920 26.199 271 148 9 28 274 17 259 1.87e-13 69.7
MS.gene03986.t1 MTR_5g078210 28.689 244 133 11 48 274 29 248 2.49e-13 69.3
MS.gene03986.t1 MTR_4g087985 28.926 242 146 9 44 274 36 262 3.06e-13 69.3
MS.gene03986.t1 MTR_8g014910 26.335 281 165 9 2 274 9 255 3.23e-13 69.3
MS.gene03986.t1 MTR_5g078210 28.151 238 142 10 48 274 29 248 3.87e-13 68.9
MS.gene03986.t1 MTR_2g033920 26.199 271 148 9 28 274 17 259 4.49e-13 68.9
MS.gene03986.t1 MTR_2g461580 25.896 251 159 7 35 275 22 255 1.02e-12 67.8
MS.gene03986.t1 MTR_2g017875 28.507 221 136 9 30 238 20 230 1.33e-12 67.4
MS.gene03986.t1 MTR_2g015080 27.600 250 156 11 33 271 29 264 1.90e-12 67.0
MS.gene03986.t1 MTR_5g009100 42.169 83 47 1 46 127 48 130 2.18e-12 67.0
MS.gene03986.t1 MTR_3g082830 34.437 151 90 6 44 192 38 181 2.51e-12 66.6
MS.gene03986.t1 MTR_3g114090 31.280 211 117 9 42 238 34 230 3.86e-12 65.9
MS.gene03986.t1 MTR_7g116520 28.235 255 145 13 36 274 26 258 7.01e-12 65.5
MS.gene03986.t1 MTR_5g093090 28.636 220 128 9 35 238 21 227 7.07e-12 65.1
MS.gene03986.t1 MTR_2g100620 27.347 245 143 11 44 274 31 254 7.60e-12 65.1
MS.gene03986.t1 MTR_7g104220 28.899 218 124 8 28 238 26 219 8.78e-12 65.1
MS.gene03986.t1 MTR_5g093090 29.224 219 128 9 35 238 21 227 1.45e-11 64.3
MS.gene03986.t1 MTR_1g025680 29.268 246 138 11 44 274 34 258 1.59e-11 64.3
MS.gene03986.t1 MTR_8g087870 26.667 240 151 9 44 274 29 252 1.76e-11 63.9
MS.gene03986.t1 MTR_2g084585 29.130 230 130 11 44 257 30 242 1.80e-11 63.9
MS.gene03986.t1 MTR_1g024890 27.823 248 155 11 36 274 24 256 2.19e-11 63.9
MS.gene03986.t1 MTR_8g087890 25.714 245 143 8 46 274 33 254 2.26e-11 63.9
MS.gene03986.t1 MTR_2g018015 28.169 213 132 9 30 232 24 225 2.39e-11 63.2
MS.gene03986.t1 MTR_8g075230 34.118 170 87 7 44 203 36 190 2.60e-11 63.5
MS.gene03986.t1 MTR_1g060490 28.571 203 119 8 41 238 34 215 3.92e-11 63.2
MS.gene03986.t1 MTR_3g435540 28.641 206 123 8 46 238 25 219 5.90e-11 62.4
MS.gene03986.t1 MTR_0565s0010 24.686 239 154 9 46 274 34 256 8.66e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03986.t1 AT5G45910 45.635 252 131 4 29 274 15 266 1.15e-67 214
MS.gene03986.t1 AT1G28610 44.492 236 124 5 43 274 30 262 3.78e-60 195
MS.gene03986.t1 AT1G28600 42.857 252 134 7 29 274 15 262 3.49e-58 191
MS.gene03986.t1 AT1G28570 43.277 238 127 6 42 274 32 266 7.99e-57 187
MS.gene03986.t1 AT1G28640 42.017 238 130 6 42 274 33 267 2.38e-56 186
MS.gene03986.t1 AT1G28590 43.277 238 127 6 42 274 34 268 4.69e-56 185
MS.gene03986.t1 AT1G31550 41.841 239 128 6 42 274 34 267 2.54e-54 181
MS.gene03986.t1 AT1G31550 41.841 239 128 6 42 274 34 267 2.91e-54 181
MS.gene03986.t1 AT1G28670 42.259 239 129 7 42 274 33 268 6.96e-54 179
MS.gene03986.t1 AT1G28580 42.017 238 130 6 42 274 35 269 9.72e-54 179
MS.gene03986.t1 AT2G27360 40.586 239 133 7 42 274 31 266 7.19e-53 177
MS.gene03986.t1 AT1G28660 42.017 238 130 6 42 274 33 267 8.42e-53 176
MS.gene03986.t1 AT1G28660 42.437 238 128 7 42 274 33 266 6.43e-52 174
MS.gene03986.t1 AT1G28650 40.336 238 134 6 42 274 35 269 1.07e-51 174
MS.gene03986.t1 AT1G28600 44.693 179 91 5 42 216 29 203 7.36e-44 149
MS.gene03986.t1 AT1G28610 44.000 175 92 4 43 214 30 201 6.88e-41 142
MS.gene03986.t1 AT3G48460 35.628 247 132 10 41 274 35 267 5.32e-35 129
MS.gene03986.t1 AT1G28580 36.898 187 113 4 90 274 5 188 5.97e-32 120
MS.gene03986.t1 AT5G03980 33.468 248 120 6 29 274 14 218 7.95e-32 120
MS.gene03986.t1 AT1G54790 33.333 255 137 9 35 274 23 259 1.29e-29 115
MS.gene03986.t1 AT4G01130 35.102 245 133 9 42 274 32 262 3.37e-29 113
MS.gene03986.t1 AT4G01130 34.836 244 135 8 42 274 32 262 8.16e-29 113
MS.gene03986.t1 AT1G67830 32.143 252 146 9 35 274 21 259 9.82e-27 107
MS.gene03986.t1 AT1G56670 30.903 288 158 13 1 274 1 261 1.44e-26 105
MS.gene03986.t1 AT3G26430 32.692 260 144 10 30 274 17 260 3.22e-26 106
MS.gene03986.t1 AT1G56670 30.903 288 158 13 1 274 1 261 7.87e-26 105
MS.gene03986.t1 AT5G14450 30.645 248 143 8 42 274 39 272 9.42e-24 99.4
MS.gene03986.t1 AT1G54790 30.534 262 147 10 30 274 15 258 2.63e-23 98.2
MS.gene03986.t1 AT1G54790 30.534 262 147 10 30 274 15 258 3.86e-23 98.2
MS.gene03986.t1 AT3G05180 30.800 250 144 10 37 274 31 263 1.79e-21 93.2
MS.gene03986.t1 AT1G09390 31.897 232 145 8 45 274 38 258 5.68e-21 91.7
MS.gene03986.t1 AT3G27950 29.572 257 144 6 34 274 22 257 9.95e-21 90.9
MS.gene03986.t1 AT3G62280 28.205 234 149 8 45 274 37 255 2.07e-20 89.0
MS.gene03986.t1 AT5G08460 29.644 253 151 8 33 274 38 274 2.95e-20 89.7
MS.gene03986.t1 AT3G62280 28.205 234 149 8 45 274 37 255 5.26e-20 89.0
MS.gene03986.t1 AT1G28570 38.318 107 64 2 170 274 88 194 1.55e-17 81.3
MS.gene03986.t1 AT1G28570 58.929 56 20 2 42 94 32 87 4.93e-13 68.6
MS.gene03986.t1 AT1G53920 37.500 152 76 6 44 189 48 186 3.70e-17 80.9
MS.gene03986.t1 AT1G28570 39.604 101 59 2 176 274 3 103 1.13e-16 77.4
MS.gene03986.t1 AT1G71120 30.290 241 136 9 44 274 31 249 1.66e-16 79.0
MS.gene03986.t1 AT1G53940 38.806 134 63 4 44 171 39 159 3.49e-16 77.4
MS.gene03986.t1 AT4G10950 27.734 256 153 9 39 275 65 307 4.66e-16 77.8
MS.gene03986.t1 AT1G53940 40.157 127 57 4 44 164 39 152 7.12e-16 77.0
MS.gene03986.t1 AT5G15720 25.714 245 150 8 44 274 30 256 1.49e-15 75.5
MS.gene03986.t1 AT5G41890 30.244 205 123 7 46 238 33 229 1.90e-15 75.9
MS.gene03986.t1 AT5G15720 25.714 245 150 8 44 274 30 256 2.14e-15 75.5
MS.gene03986.t1 AT5G40990 35.260 173 86 7 44 201 37 198 2.56e-15 75.5
MS.gene03986.t1 AT5G45950 28.922 204 125 7 44 238 40 232 3.22e-14 72.0
MS.gene03986.t1 AT3G09930 29.293 198 118 9 45 238 40 219 4.38e-14 71.6
MS.gene03986.t1 AT3G09930 29.293 198 118 9 45 238 40 219 4.38e-14 71.6
MS.gene03986.t1 AT1G71691 28.638 213 126 6 44 244 55 253 7.92e-14 70.9
MS.gene03986.t1 AT2G03980 28.111 217 133 7 42 249 41 243 1.12e-13 70.5
MS.gene03986.t1 AT2G03980 28.111 217 133 7 42 249 41 243 1.12e-13 70.5
MS.gene03986.t1 AT2G03980 28.111 217 133 7 42 249 41 243 1.12e-13 70.5
MS.gene03986.t1 AT2G03980 28.111 217 133 7 42 249 41 243 1.12e-13 70.5
MS.gene03986.t1 AT5G45960 30.622 209 128 7 44 244 47 246 1.13e-13 70.9
MS.gene03986.t1 AT3G14225 30.332 211 127 8 44 243 37 238 1.42e-13 70.5
MS.gene03986.t1 AT3G14225 30.144 209 126 8 44 243 37 234 1.52e-13 70.5
MS.gene03986.t1 AT4G10950 27.344 256 150 10 39 275 65 303 1.61e-13 70.5
MS.gene03986.t1 AT2G42990 29.289 239 146 9 44 274 28 251 1.70e-13 70.1
MS.gene03986.t1 AT1G71250 27.363 201 134 4 44 238 41 235 1.74e-13 70.1
MS.gene03986.t1 AT1G71691 28.774 212 125 6 44 243 55 252 1.79e-13 70.1
MS.gene03986.t1 AT1G20120 30.833 240 145 8 42 274 76 301 1.19e-12 67.8
MS.gene03986.t1 AT1G74460 29.612 206 121 8 46 238 25 219 1.28e-12 67.4
MS.gene03986.t1 AT4G26790 29.557 203 130 8 42 238 27 222 1.96e-12 67.0
MS.gene03986.t1 AT4G26790 29.557 203 130 8 42 238 27 222 1.96e-12 67.0
MS.gene03986.t1 AT5G63170 26.087 253 148 9 36 274 22 249 5.40e-12 65.5
MS.gene03986.t1 AT2G19010 30.000 210 116 11 46 243 32 222 7.01e-12 65.1
MS.gene03986.t1 AT3G04290 28.333 240 150 8 43 274 28 253 8.31e-12 65.1
MS.gene03986.t1 AT2G19010 30.000 210 116 11 46 243 62 252 8.72e-12 65.1
MS.gene03986.t1 AT2G04020 28.182 220 130 9 42 249 41 244 1.49e-11 63.5
MS.gene03986.t1 AT2G04570 28.000 250 151 10 35 274 21 251 2.13e-11 63.9
MS.gene03986.t1 AT2G19060 26.778 239 141 8 46 274 32 246 2.14e-11 63.9
MS.gene03986.t1 AT1G75880 26.568 271 178 8 10 274 21 276 3.06e-11 63.5
MS.gene03986.t1 AT1G75890 27.126 247 155 9 44 274 47 284 4.13e-11 63.2
MS.gene03986.t1 AT2G04020 29.665 209 119 9 42 238 41 233 7.35e-11 62.0
MS.gene03986.t1 AT4G16230 28.502 207 125 8 44 238 30 225 8.16e-11 61.2

Find 45 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACGAAGCCATCTTCAATTT+TGG 0.178136 3.2:+54611361 MS.gene03986:CDS
CACCCAATTGGAAAGTTTCC+AGG 0.330852 3.2:-54612356 None:intergenic
ATGCCTGACAATAGTCCTTA+TGG 0.355804 3.2:+54611433 MS.gene03986:CDS
CCCCTTTCTTCGTGTCTCCT+TGG 0.374519 3.2:-54611771 None:intergenic
CGAAAGCATTGTAAGCTATC+AGG 0.379885 3.2:-54612442 None:intergenic
AAGTTGTTCATTGTAGTATT+CGG 0.396518 3.2:-54612465 None:intergenic
ATTCCTGGAAACTTTCCAAT+TGG 0.402546 3.2:+54612353 MS.gene03986:CDS
TTGTTTATAGTGGGAGAGAT+TGG 0.429569 3.2:+54612061 MS.gene03986:CDS
AATGTTGTTTCAATTTCAAA+TGG 0.430898 3.2:+54611292 MS.gene03986:CDS
GCAATGGAGCTAGTGATTCC+TGG 0.440053 3.2:+54612338 MS.gene03986:CDS
CTTCAGTCATTAATCAAAGA+AGG 0.446875 3.2:+54612314 MS.gene03986:intron
TTCCTGGAAACTTTCCAATT+GGG 0.455772 3.2:+54612354 MS.gene03986:CDS
ACACCAGCATTACACCCAAT+TGG 0.462222 3.2:-54612368 None:intergenic
AGAATCACCAAAATTGAAGA+TGG 0.466360 3.2:-54611368 None:intergenic
GCCAAGGAGACACGAAGAAA+GGG 0.468871 3.2:+54611770 MS.gene03986:CDS
GTGTAATAGATTCAACCAAT+GGG 0.476700 3.2:-54612147 None:intergenic
AAGGAAGACTATGATGAACT+TGG 0.478477 3.2:+54612416 MS.gene03986:CDS
GGCTTCGTAAGGTAAAGAAT+TGG 0.479011 3.2:-54611347 None:intergenic
ACTTACTTCAAACATCCATC+TGG 0.482860 3.2:+54611460 MS.gene03986:CDS
TTATAGTGGGAGAGATTGGT+GGG 0.487433 3.2:+54612065 MS.gene03986:CDS
TTGAAGTAAGTTGAGCCATA+AGG 0.494864 3.2:-54611448 None:intergenic
TTTCCAATTGGGTGTAATGC+TGG 0.500453 3.2:+54612365 MS.gene03986:CDS
AAATGTGATTAGTAAGAAGA+AGG 0.505122 3.2:+54612397 MS.gene03986:CDS
TTTATAGTGGGAGAGATTGG+TGG 0.512093 3.2:+54612064 MS.gene03986:CDS
AGCCAAGGAGACACGAAGAA+AGG 0.513729 3.2:+54611769 MS.gene03986:CDS
GAATGATCTGTGGTGTTTAG+TGG 0.523875 3.2:-54611859 None:intergenic
TTGTTGACATTCATATATGC+CGG 0.525369 3.2:-54611745 None:intergenic
GAGCCATAAGGACTATTGTC+AGG 0.532224 3.2:-54611436 None:intergenic
ACTATTTATTTCGCTCGTCG+TGG 0.535853 3.2:+54611829 MS.gene03986:CDS
TTAATTGTAGCTGAGGCATA+CGG 0.536180 3.2:+54611709 MS.gene03986:intron
AGTGTAATAGATTCAACCAA+TGG 0.543554 3.2:-54612148 None:intergenic
GGTGATTCTATAAGTGACAC+TGG 0.553229 3.2:+54611382 MS.gene03986:CDS
AAAACCACATGTGAAGGTGA+TGG 0.559909 3.2:-54611263 None:intergenic
ATTAATCAAAGAAGGAGCAA+TGG 0.563591 3.2:+54612322 MS.gene03986:CDS
CATTGCGTGTGCAACTTGAG+TGG 0.582672 3.2:+54611881 MS.gene03986:CDS
CCATTCGATAAACGTCCAGA+TGG 0.592371 3.2:-54611475 None:intergenic
CCATCTGGACGTTTATCGAA+TGG 0.593553 3.2:+54611475 MS.gene03986:CDS
AAATTGAAGATGGCTTCGTA+AGG 0.607757 3.2:-54611358 None:intergenic
ATGAATGTCAACAATAGCCA+AGG 0.610103 3.2:+54611754 MS.gene03986:CDS
AGGAAGACTATGATGAACTT+GGG 0.616167 3.2:+54612417 MS.gene03986:CDS
CCAAGGAGACACGAAGAAAG+GGG 0.652855 3.2:+54611771 MS.gene03986:CDS
ATTGATATAACATACGATGG+TGG 0.657430 3.2:-54612173 None:intergenic
TGTAATAGATTCAACCAATG+GGG 0.661230 3.2:-54612146 None:intergenic
GTAATAGATTCAACCAATGG+GGG 0.681881 3.2:-54612145 None:intergenic
CACACGCAATGAATGATCTG+TGG 0.710194 3.2:-54611869 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGAAAGTAAAAAAAAAAA+TGG + chr3.2:54611568-54611587 MS.gene03986:intron 10.0%
!! AAAAAATCATTGTTTATAGT+GGG + chr3.2:54612052-54612071 MS.gene03986:CDS 15.0%
!! TTAAAACAAACAAAATATGT+AGG - chr3.2:54611962-54611981 None:intergenic 15.0%
!! AAAATTGATATAACATACGA+TGG - chr3.2:54612179-54612198 None:intergenic 20.0%
!! ATTCTTGGATAAATGTAATA+GGG + chr3.2:54612266-54612285 MS.gene03986:intron 20.0%
!! CAAAAAATCATTGTTTATAG+TGG + chr3.2:54612051-54612070 MS.gene03986:CDS 20.0%
!! GACTAAATGAATATTATTCT+TGG + chr3.2:54612251-54612270 MS.gene03986:intron 20.0%
!! TATTCTTGGATAAATGTAAT+AGG + chr3.2:54612265-54612284 MS.gene03986:intron 20.0%
!!! AATGTTGTTTCAATTTCAAA+TGG + chr3.2:54611292-54611311 MS.gene03986:CDS 20.0%
!!! TTAATTGAGCTTAGTTTTTA+TGG - chr3.2:54612225-54612244 None:intergenic 20.0%
! AAATGTGATTAGTAAGAAGA+AGG + chr3.2:54612397-54612416 MS.gene03986:CDS 25.0%
! AAGTTGTTCATTGTAGTATT+CGG - chr3.2:54612468-54612487 None:intergenic 25.0%
! TTCATGTGTAAAAGCAAAAA+AGG + chr3.2:54611922-54611941 MS.gene03986:CDS 25.0%
!! CGACTGATTATAGATTTTAT+AGG + chr3.2:54611499-54611518 MS.gene03986:CDS 25.0%
!!! AAAAGTTGTGTTATTTCATG+AGG - chr3.2:54611998-54612017 None:intergenic 25.0%
!!! TTTTTGCTTTTACACATGAA+TGG - chr3.2:54611922-54611941 None:intergenic 25.0%
AAAATGGCAATGCTAGAAAA+TGG + chr3.2:54611584-54611603 MS.gene03986:intron 30.0%
AGAATCACCAAAATTGAAGA+TGG - chr3.2:54611371-54611390 None:intergenic 30.0%
AGTGTAATAGATTCAACCAA+TGG - chr3.2:54612151-54612170 None:intergenic 30.0%
ATTAATCAAAGAAGGAGCAA+TGG + chr3.2:54612322-54612341 MS.gene03986:CDS 30.0%
ATTGATATAACATACGATGG+TGG - chr3.2:54612176-54612195 None:intergenic 30.0%
CTTCAGTCATTAATCAAAGA+AGG + chr3.2:54612314-54612333 MS.gene03986:intron 30.0%
GTGTAATAGATTCAACCAAT+GGG - chr3.2:54612150-54612169 None:intergenic 30.0%
TGTAATAGATTCAACCAATG+GGG - chr3.2:54612149-54612168 None:intergenic 30.0%
TTGTTGACATTCATATATGC+CGG - chr3.2:54611748-54611767 None:intergenic 30.0%
! ATGGCAATGAGATTTTTGTT+CGG + chr3.2:54611311-54611330 MS.gene03986:CDS 30.0%
!! ACACAACTTTTGTTATGATG+TGG + chr3.2:54612007-54612026 MS.gene03986:intron 30.0%
!! CACAACTTTTGTTATGATGT+GGG + chr3.2:54612008-54612027 MS.gene03986:intron 30.0%
!!! ACTCATTTTAATTGTAGCTG+AGG + chr3.2:54611702-54611721 MS.gene03986:intron 30.0%
AAATTGAAGATGGCTTCGTA+AGG - chr3.2:54611361-54611380 None:intergenic 35.0%
AAGGAAGACTATGATGAACT+TGG + chr3.2:54612416-54612435 MS.gene03986:CDS 35.0%
ACTTACTTCAAACATCCATC+TGG + chr3.2:54611460-54611479 MS.gene03986:CDS 35.0%
AGGAAGACTATGATGAACTT+GGG + chr3.2:54612417-54612436 MS.gene03986:CDS 35.0%
ATGAATGTCAACAATAGCCA+AGG + chr3.2:54611754-54611773 MS.gene03986:CDS 35.0%
ATTCCTGGAAACTTTCCAAT+TGG + chr3.2:54612353-54612372 MS.gene03986:CDS 35.0%
GTAATAGATTCAACCAATGG+GGG - chr3.2:54612148-54612167 None:intergenic 35.0%
TCCAAAAAAACCACATGTGA+AGG - chr3.2:54611272-54611291 None:intergenic 35.0%
TTAATTGTAGCTGAGGCATA+CGG + chr3.2:54611709-54611728 MS.gene03986:intron 35.0%
TTCATATATGCCGGCAAAAA+TGG - chr3.2:54611739-54611758 None:intergenic 35.0%
TTCCTGGAAACTTTCCAATT+GGG + chr3.2:54612354-54612373 MS.gene03986:CDS 35.0%
! TACGAAGCCATCTTCAATTT+TGG + chr3.2:54611361-54611380 MS.gene03986:CDS 35.0%
! TGGCAATGAGATTTTTGTTC+GGG + chr3.2:54611312-54611331 MS.gene03986:CDS 35.0%
! TTGAAGTAAGTTGAGCCATA+AGG - chr3.2:54611451-54611470 None:intergenic 35.0%
! TTGTTTATAGTGGGAGAGAT+TGG + chr3.2:54612061-54612080 MS.gene03986:CDS 35.0%
!! ATACGGATTACCATTTTTGC+CGG + chr3.2:54611726-54611745 MS.gene03986:CDS 35.0%
!!! ACCTTCACATGTGGTTTTTT+TGG + chr3.2:54611268-54611287 MS.gene03986:CDS 35.0%
AAAACCACATGTGAAGGTGA+TGG - chr3.2:54611266-54611285 None:intergenic 40.0%
AAGAAAAGTTGTTCCCCCAT+TGG + chr3.2:54612132-54612151 MS.gene03986:CDS 40.0%
ACTATTTATTTCGCTCGTCG+TGG + chr3.2:54611829-54611848 MS.gene03986:CDS 40.0%
ATGCCTGACAATAGTCCTTA+TGG + chr3.2:54611433-54611452 MS.gene03986:CDS 40.0%
GAATGATCTGTGGTGTTTAG+TGG - chr3.2:54611862-54611881 None:intergenic 40.0%
GGCTTCGTAAGGTAAAGAAT+TGG - chr3.2:54611350-54611369 None:intergenic 40.0%
GGTGATTCTATAAGTGACAC+TGG + chr3.2:54611382-54611401 MS.gene03986:CDS 40.0%
TTTCCAATTGGGTGTAATGC+TGG + chr3.2:54612365-54612384 MS.gene03986:CDS 40.0%
! GGGGTAAATTTTGCATTTGC+AGG + chr3.2:54611790-54611809 MS.gene03986:CDS 40.0%
! TTATAGTGGGAGAGATTGGT+GGG + chr3.2:54612065-54612084 MS.gene03986:CDS 40.0%
! TTTATAGTGGGAGAGATTGG+TGG + chr3.2:54612064-54612083 MS.gene03986:CDS 40.0%
! TTTTCCATCACCTTCACATG+TGG + chr3.2:54611259-54611278 MS.gene03986:CDS 40.0%
!! CGAAAGCATTGTAAGCTATC+AGG - chr3.2:54612445-54612464 None:intergenic 40.0%
ACACCAGCATTACACCCAAT+TGG - chr3.2:54612371-54612390 None:intergenic 45.0%
CACACGCAATGAATGATCTG+TGG - chr3.2:54611872-54611891 None:intergenic 45.0%
CACCCAATTGGAAAGTTTCC+AGG - chr3.2:54612359-54612378 None:intergenic 45.0%
CCATCTGGACGTTTATCGAA+TGG + chr3.2:54611475-54611494 MS.gene03986:CDS 45.0%
CCATTCGATAAACGTCCAGA+TGG - chr3.2:54611478-54611497 None:intergenic 45.0%
GAGCCATAAGGACTATTGTC+AGG - chr3.2:54611439-54611458 None:intergenic 45.0%
AGCCAAGGAGACACGAAGAA+AGG + chr3.2:54611769-54611788 MS.gene03986:CDS 50.0%
CATTGCGTGTGCAACTTGAG+TGG + chr3.2:54611881-54611900 MS.gene03986:CDS 50.0%
CCAAGGAGACACGAAGAAAG+GGG + chr3.2:54611771-54611790 MS.gene03986:CDS 50.0%
GCAATGGAGCTAGTGATTCC+TGG + chr3.2:54612338-54612357 MS.gene03986:CDS 50.0%
GCCAAGGAGACACGAAGAAA+GGG + chr3.2:54611770-54611789 MS.gene03986:CDS 50.0%
CCCCTTTCTTCGTGTCTCCT+TGG - chr3.2:54611774-54611793 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 54611238 54612493 54611238 ID=MS.gene03986
chr3.2 mRNA 54611238 54612493 54611238 ID=MS.gene03986.t1;Parent=MS.gene03986
chr3.2 exon 54611238 54611520 54611238 ID=MS.gene03986.t1.exon1;Parent=MS.gene03986.t1
chr3.2 CDS 54611238 54611520 54611238 ID=cds.MS.gene03986.t1;Parent=MS.gene03986.t1
chr3.2 exon 54611719 54611943 54611719 ID=MS.gene03986.t1.exon2;Parent=MS.gene03986.t1
chr3.2 CDS 54611719 54611943 54611719 ID=cds.MS.gene03986.t1;Parent=MS.gene03986.t1
chr3.2 exon 54612035 54612180 54612035 ID=MS.gene03986.t1.exon3;Parent=MS.gene03986.t1
chr3.2 CDS 54612035 54612180 54612035 ID=cds.MS.gene03986.t1;Parent=MS.gene03986.t1
chr3.2 exon 54612320 54612493 54612320 ID=MS.gene03986.t1.exon4;Parent=MS.gene03986.t1
chr3.2 CDS 54612320 54612493 54612320 ID=cds.MS.gene03986.t1;Parent=MS.gene03986.t1
Gene Sequence

>MS.gene03986

ATGGTGATGATATTCATTATCTTTTCCATCACCTTCACATGTGGTTTTTTTGGAAATGTTGTTTCAATTTCAAATGGCAATGAGATTTTTGTTCGGGATGAGTCATATGCCAATTCTTTACCTTACGAAGCCATCTTCAATTTTGGTGATTCTATAAGTGACACTGGAAATGCTGCTGCATTTAGCGACAAACATATGCCTGACAATAGTCCTTATGGCTCAACTTACTTCAAACATCCATCTGGACGTTTATCGAATGGACGACTGATTATAGATTTTATAGGTAATTTTACTCTTCTTTATTTAAGTTTGTTCAATAAGATTTTCTAATATGAAAGTAAAAAAAAAAATGGCAATGCTAGAAAATGGTATAACATGCTCTAGTTAATATAGTTTGTTAGAATCGTCAAAATTATTAAATCTTAAAGTCAATATATTTATGATTGAAAAATTTACAAGATTGTACTCATTTTAATTGTAGCTGAGGCATACGGATTACCATTTTTGCCGGCATATATGAATGTCAACAATAGCCAAGGAGACACGAAGAAAGGGGTAAATTTTGCATTTGCAGGTGCTAGTGCACTTAAAACTATTTATTTCGCTCGTCGTGGAATGATGCCACTAAACACCACAGATCATTCATTGCGTGTGCAACTTGAGTGGTTTAAGAAACTAAAGCCATTCATGTGTAAAAGCAAAAAAGGTTTCATTATAACTACCTACATATTTTGTTTGTTTTAAATATATAAATCTTCCTCATGAAATAACACAACTTTTGTTATGATGTGGGACAGAATGTACTAGCTACTTCAAAAAATCATTGTTTATAGTGGGAGAGATTGGTGGGAATGATGTTCTGCAGTATATCTCATCTAAAAAGTTTGCAGAAATAAGAAAAGTTGTTCCCCCATTGGTTGAATCTATTACACTTACCACCATCGTATGTTATATCAATTTTAAGTACTTGAATGTCTTCTTTAACCATAAAAACTAAGCTCAATTAAAGATATGACTAAATGAATATTATTCTTGGATAAATGTAATAGGGATTTTTTTTCTTTCTATTTTTTTATCTTCAGTCATTAATCAAAGAAGGAGCAATGGAGCTAGTGATTCCTGGAAACTTTCCAATTGGGTGTAATGCTGGTGTTTTGACAAATGTGATTAGTAAGAAGAAGGAAGACTATGATGAACTTGGGTGCCTGATAGCTTACAATGCTTTCGCCGAATACTACAATGAACAACTTCCATAG
Protein sequence

>MS.gene03986.t1

MVMIFIIFSITFTCGFFGNVVSISNGNEIFVRDESYANSLPYEAIFNFGDSISDTGNAAAFSDKHMPDNSPYGSTYFKHPSGRLSNGRLIIDFIAEAYGLPFLPAYMNVNNSQGDTKKGVNFAFAGASALKTIYFARRGMMPLNTTDHSLRVQLEWFKKLKPFMCKSKKECTSYFKKSLFIVGEIGGNDVLQYISSKKFAEIRKVVPPLVESITLTTISLIKEGAMELVIPGNFPIGCNAGVLTNVISKKKEDYDELGCLIAYNAFAEYYNEQLP