AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040422

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040422.t1 MTR_3g101030 80.706 425 65 3 39 446 5 429 0.0 678
MS.gene040422.t1 MTR_3g101050 72.991 448 67 7 1 445 1 397 0.0 607
MS.gene040422.t1 MTR_3g101010 80.679 383 70 2 66 446 9 389 0.0 598
MS.gene040422.t1 MTR_8g027395 67.000 400 121 4 49 446 7 397 0.0 524
MS.gene040422.t1 MTR_3g018740 69.974 383 108 3 65 444 8 386 0.0 521
MS.gene040422.t1 MTR_4g045707 67.347 392 120 5 57 446 3 388 1.54e-179 507
MS.gene040422.t1 MTR_8g461160 65.816 392 130 2 57 444 1 392 1.18e-177 502
MS.gene040422.t1 MTR_8g027420 66.494 385 110 5 64 446 8 375 5.57e-175 494
MS.gene040422.t1 MTR_3g101180 64.573 398 129 4 57 444 3 398 4.04e-173 491
MS.gene040422.t1 MTR_3g048000 63.402 388 117 5 60 444 25 390 1.02e-168 479
MS.gene040422.t1 MTR_4g088525 65.940 367 117 3 81 445 8 368 4.34e-167 474
MS.gene040422.t1 MTR_7g451890 62.078 385 142 2 66 446 18 402 5.68e-167 475
MS.gene040422.t1 MTR_4g088535 65.026 386 109 6 58 441 5 366 3.11e-165 470
MS.gene040422.t1 MTR_7g050810 61.697 389 137 3 66 446 20 404 2.39e-164 469
MS.gene040422.t1 MTR_2g046030 63.896 385 126 5 66 444 9 386 3.20e-164 468
MS.gene040422.t1 MTR_7g047780 62.338 385 141 2 66 446 18 402 5.00e-164 468
MS.gene040422.t1 MTR_2g045980 63.896 385 126 5 66 444 9 386 1.02e-163 467
MS.gene040422.t1 MTR_7g050750 61.299 385 145 2 66 446 12 396 7.97e-162 462
MS.gene040422.t1 MTR_3g101130 60.622 386 148 3 62 446 7 389 2.11e-160 458
MS.gene040422.t1 MTR_3g015620 60.938 384 120 6 66 444 20 378 2.72e-150 432
MS.gene040422.t1 MTR_3g102920 58.575 379 117 4 70 444 66 408 1.64e-139 406
MS.gene040422.t1 MTR_3g100995 75.294 255 62 1 192 446 11 264 5.69e-139 399
MS.gene040422.t1 MTR_3g101200 55.556 369 139 4 78 444 45 390 6.96e-127 373
MS.gene040422.t1 MTR_3g101190 54.076 368 102 4 83 446 8 312 2.96e-124 363
MS.gene040422.t1 MTR_3g018747 68.846 260 63 3 188 444 9 253 5.39e-121 353
MS.gene040422.t1 MTR_3g100010 58.075 322 87 2 85 373 38 344 2.78e-116 344
MS.gene040422.t1 MTR_7g050830 55.340 309 107 2 68 356 22 319 1.80e-111 331
MS.gene040422.t1 MTR_3g015760 58.423 279 102 4 173 444 8 279 1.08e-98 297
MS.gene040422.t1 MTR_3g111160 68.571 210 61 2 240 446 13 220 1.63e-90 274
MS.gene040422.t1 MTR_3g100520 60.811 222 70 3 229 446 1 209 2.70e-86 263
MS.gene040422.t1 MTR_3g101110 60.251 239 63 4 188 420 68 280 4.33e-86 265
MS.gene040422.t1 MTR_3g114310 42.250 400 204 12 64 446 21 410 2.20e-85 268
MS.gene040422.t1 MTR_3g099970 66.667 195 48 2 188 381 20 198 8.84e-84 256
MS.gene040422.t1 MTR_2g046410 64.532 203 64 3 248 444 147 347 1.38e-79 250
MS.gene040422.t1 MTR_3g101020 66.486 185 37 2 262 446 1 160 2.94e-76 235
MS.gene040422.t1 MTR_3g111150 58.757 177 47 2 66 242 3 153 6.20e-62 198
MS.gene040422.t1 MTR_3g101150 62.420 157 53 2 294 444 1 157 1.26e-59 192
MS.gene040422.t1 MTR_3g102870 57.764 161 66 1 251 409 1 161 1.09e-56 185
MS.gene040422.t1 MTR_3g100990 58.784 148 58 2 66 213 9 153 2.19e-44 162
MS.gene040422.t1 MTR_4g093550 63.415 82 28 1 66 147 10 89 1.65e-24 97.4
MS.gene040422.t1 MTR_6g046890 64.474 76 24 1 80 155 15 87 5.80e-20 86.3
MS.gene040422.t1 MTR_3g101140 61.538 65 24 1 383 446 52 116 2.45e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040422.t1 AT1G47710 51.852 405 186 5 44 444 18 417 9.36e-143 414
MS.gene040422.t1 AT1G47710 52.551 392 178 4 57 444 3 390 2.16e-142 412
MS.gene040422.t1 AT3G45220 48.072 389 184 6 66 443 9 390 5.92e-120 355
MS.gene040422.t1 AT2G25240 47.656 384 185 7 66 443 9 382 6.55e-115 342
MS.gene040422.t1 AT2G26390 46.770 387 188 6 66 443 9 386 5.24e-113 338
MS.gene040422.t1 AT1G62170 42.779 367 183 4 85 445 88 433 5.13e-96 296
MS.gene040422.t1 AT2G14540 44.792 384 183 6 66 443 37 397 7.87e-95 291
MS.gene040422.t1 AT1G64030 42.708 384 193 7 66 443 9 371 7.10e-92 283
MS.gene040422.t1 AT2G35580 41.823 373 188 8 81 443 18 371 4.57e-89 276
MS.gene040422.t1 AT1G62170 39.250 400 183 5 85 445 88 466 4.75e-89 279
MS.gene040422.t1 AT1G64010 46.759 216 93 4 229 443 1 195 1.12e-59 194
MS.gene040422.t1 AT1G64010 46.766 201 85 4 244 443 2 181 1.18e-53 177
MS.gene040422.t1 AT1G62160 40.110 182 86 4 266 445 60 220 9.74e-35 129
MS.gene040422.t1 AT1G63280 52.174 115 51 2 188 300 8 120 4.69e-32 119
MS.gene040422.t1 AT1G51330 49.550 111 53 2 187 294 30 140 5.52e-24 99.4
MS.gene040422.t1 AT1G64020 32.432 111 72 3 70 179 13 121 2.13e-12 64.3

Find 102 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAATGCGCTGTACTTTAA+AGG 0.166787 7.2:+69060969 MS.gene040422:CDS
TGACCAATGAAGTGAATTTA+TGG 0.210594 7.2:+69060868 MS.gene040422:CDS
TTATCTTCACCTTGCTTATA+AGG 0.268250 7.2:-69061137 None:intergenic
CTTGTCTTGATAAGATTCTT+TGG 0.269178 7.2:-69060388 None:intergenic
CAATGGAGGTCTTACTAAAA+TGG 0.273021 7.2:+69061373 MS.gene040422:CDS
ATCGATTTGGATATTGCTTA+TGG 0.285955 7.2:-69060265 None:intergenic
CCAAAGTTCAATATTTCATT+TGG 0.296966 7.2:+69061299 MS.gene040422:CDS
CAGGTGCTCAATCCTCTTGC+TGG 0.306550 7.2:+69061608 MS.gene040422:CDS
GTTGAGTTGATCGGTGGATT+TGG 0.319346 7.2:-69060511 None:intergenic
CAAATGAAATATTGAACTTT+GGG 0.323605 7.2:-69061298 None:intergenic
GCCAACTTTGCTTGAGAAGC+TGG 0.324647 7.2:+69061208 MS.gene040422:CDS
ACTGAAACAATCATCTTTGT+TGG 0.329190 7.2:+69061584 MS.gene040422:CDS
GGCTTCCGAATCTGAGTTAC+TGG 0.338294 7.2:+69061229 MS.gene040422:CDS
AGCCCATAAATTCACTTCAT+TGG 0.342189 7.2:-69060871 None:intergenic
TATCTTTCGTCGATGGTGTA+TGG 0.345495 7.2:+69060598 MS.gene040422:CDS
AAGTGGAAGTAGGTGACTTT+AGG 0.367192 7.2:+69061273 MS.gene040422:CDS
TTCCTTCCAAATGCAAAAGA+TGG 0.367541 7.2:+69061182 MS.gene040422:CDS
CCAAATGAAATATTGAACTT+TGG 0.376715 7.2:-69061299 None:intergenic
TTCTTTGGAGAAAAGATGTT+TGG 0.376879 7.2:-69060373 None:intergenic
TGCGGTGGCTGCAACAGCTT+CGG 0.384988 7.2:-69061468 None:intergenic
ATTCAAAAGGTGAAAATCAT+AGG 0.391490 7.2:-69061030 None:intergenic
GAAAGAAGGTTGAAGAGAAA+GGG 0.417612 7.2:-69060631 None:intergenic
TGAAAGAAGGTTGAAGAGAA+AGG 0.419511 7.2:-69060632 None:intergenic
ATCTTATCAAGACAAGAATA+TGG 0.421750 7.2:+69060395 MS.gene040422:CDS
GACACAATTCCTTTGAAAGA+AGG 0.426001 7.2:-69060645 None:intergenic
ATTAAAGAACTTCTTCCTCT+AGG 0.427888 7.2:+69060915 MS.gene040422:CDS
TTTGAATGGAAACTCAGTTA+AGG 0.440673 7.2:+69061046 MS.gene040422:CDS
TAACTGAGTTTCCATTCAAA+AGG 0.441115 7.2:-69061043 None:intergenic
AACAATCATCTTTGTTGGAC+AGG 0.442686 7.2:+69061589 MS.gene040422:CDS
TCTCTAATCATAAACAAGAA+AGG 0.443816 7.2:-69061554 None:intergenic
TTAAAGAACTTCTTCCTCTA+GGG 0.444956 7.2:+69060916 MS.gene040422:CDS
CAAGCTTCCTCTTGATAAAG+TGG 0.447424 7.2:+69061256 MS.gene040422:CDS
GATGTTGAGTGGGATCGATT+TGG 0.447564 7.2:-69060278 None:intergenic
TTTATTGACGTAAATGAAGA+AGG 0.449783 7.2:+69061443 MS.gene040422:CDS
CTCTAATCATAAACAAGAAA+GGG 0.468439 7.2:-69061553 None:intergenic
ATCATCGCTGCTGGCTCCGA+GGG 0.472440 7.2:+69060453 MS.gene040422:CDS
TCTCTTCAACCTTCTTTCAA+AGG 0.474212 7.2:+69060636 MS.gene040422:CDS
GTGTTCCAGTAACTCAGATT+CGG 0.474362 7.2:-69061234 None:intergenic
TCCTTCCAAATGCAAAAGAT+GGG 0.485174 7.2:+69061183 MS.gene040422:CDS
GCCAGCTTCTCAAGCAAAGT+TGG 0.489801 7.2:-69061209 None:intergenic
ATCTTTCGTCGATGGTGTAT+GGG 0.491547 7.2:+69060599 MS.gene040422:CDS
GATAGATGAGGCCCGCCTGC+GGG 0.493469 7.2:-69060579 None:intergenic
GTGCTAAGCATCATCGCTGC+TGG 0.494658 7.2:+69060444 MS.gene040422:CDS
TTGAGAAGTTGTTGTTGTGT+GGG 0.494908 7.2:-69060477 None:intergenic
GACCAATGAAGTGAATTTAT+GGG 0.495263 7.2:+69060869 MS.gene040422:CDS
GTTGATCGGTGGATTTGGAT+TGG 0.496620 7.2:-69060506 None:intergenic
CTTCACCTTGCTTATAAGGA+AGG 0.497179 7.2:-69061133 None:intergenic
ATATTGCTTATGGTTCTCTT+CGG 0.500550 7.2:-69060255 None:intergenic
AGGTGCTCAATCCTCTTGCT+GGG 0.503743 7.2:+69061609 MS.gene040422:CDS
TTGTTGTTGTGTGGGGCCCT+CGG 0.506763 7.2:-69060469 None:intergenic
GTTGAGAAGTTGTTGTTGTG+TGG 0.516014 7.2:-69060478 None:intergenic
GCTATGGTTGTTTGCGAGGG+AGG 0.521590 7.2:-69060331 None:intergenic
TGCAGTCACTAGCAGTGGCA+TGG 0.521843 7.2:+69061496 MS.gene040422:CDS
GCTACAAAGTCTAGTCGAGT+TGG 0.522053 7.2:-69061524 None:intergenic
AAAGATGTTTGGCGATGGTG+AGG 0.524270 7.2:-69060362 None:intergenic
AGCACAACTTGTAGTGAAAG+TGG 0.525473 7.2:-69060426 None:intergenic
GCAGTCACTAGCAGTGGCAT+GGG 0.532869 7.2:+69061497 MS.gene040422:CDS
CTTGGCCTTCCTTATAAGCA+AGG 0.532934 7.2:+69061128 MS.gene040422:CDS
CATCATCGCTGCTGGCTCCG+AGG 0.533411 7.2:+69060452 MS.gene040422:CDS
CGCTGTACTTTAAAGGAGAG+TGG 0.534492 7.2:+69060976 MS.gene040422:CDS
GATCGGTGGATTTGGATTGG+AGG 0.535140 7.2:-69060503 None:intergenic
AATGACGGAGACGAGGATAG+AGG 0.536198 7.2:-69060541 None:intergenic
GGAGACGAGGATAGAGGCGA+AGG 0.536431 7.2:-69060535 None:intergenic
TCTGATATGCTGAAGGAGCT+AGG 0.542423 7.2:+69061332 MS.gene040422:CDS
GAAGAAGCAGAGAACAACTT+TGG 0.549721 7.2:-69060181 None:intergenic
AAAACGGTGCTCCCGCAGGC+GGG 0.550168 7.2:+69060568 MS.gene040422:CDS
CTACAAAACGGTGCTCCCGC+AGG 0.550410 7.2:+69060564 MS.gene040422:CDS
CGTTGCTATGGTTGTTTGCG+AGG 0.559095 7.2:-69060335 None:intergenic
CCTACTTCCACTTTATCAAG+AGG 0.564451 7.2:-69061263 None:intergenic
GAGAAGCTGTTGATGTTGAG+TGG 0.565808 7.2:-69060289 None:intergenic
CCTCATCTATCTTTCGTCGA+TGG 0.565945 7.2:+69060591 MS.gene040422:CDS
CCTCTTGATAAAGTGGAAGT+AGG 0.566916 7.2:+69061263 MS.gene040422:CDS
TTCTGTCGATTTCAAGAACA+AGG 0.572001 7.2:+69060692 MS.gene040422:CDS
GTCTCCGTCATTCTACAAAA+CGG 0.572969 7.2:+69060552 MS.gene040422:CDS
GTTGCTATGGTTGTTTGCGA+GGG 0.577691 7.2:-69060334 None:intergenic
TGAGAAGTTGTTGTTGTGTG+GGG 0.577961 7.2:-69060476 None:intergenic
GTAAGACCTCCATTGGTGAA+AGG 0.579413 7.2:-69061365 None:intergenic
CACTAAAATCACCCAGCAAG+AGG 0.587700 7.2:-69061620 None:intergenic
CAAAACGGTGCTCCCGCAGG+CGG 0.589050 7.2:+69060567 MS.gene040422:CDS
TTTGTAGAATGACGGAGACG+AGG 0.590002 7.2:-69060548 None:intergenic
AAGATGTTTGGCGATGGTGA+GGG 0.596429 7.2:-69060361 None:intergenic
GCAGAGAACAACTTTGGCGA+TGG 0.601250 7.2:-69060175 None:intergenic
GGCGAAGGAGTTGAGTTGAT+CGG 0.602516 7.2:-69060520 None:intergenic
TGAAACTTCTGATATGCTGA+AGG 0.602682 7.2:+69061325 MS.gene040422:CDS
AGATAGATGAGGCCCGCCTG+CGG 0.604970 7.2:-69060580 None:intergenic
TGGTGAGGGAAACGTTGCTA+TGG 0.613033 7.2:-69060347 None:intergenic
AGAACTTCTTCCTCTAGGGT+CGG 0.613652 7.2:+69060920 MS.gene040422:CDS
TTGCGAGGGAGGATTCTGAT+CGG 0.616661 7.2:-69060320 None:intergenic
CTTCTTCGTGCTCAACATCG+TGG 0.619017 7.2:+69060197 MS.gene040422:CDS
AGAAGCTGTTGATGTTGAGT+GGG 0.619685 7.2:-69060288 None:intergenic
TTTCGTCGATGGTGTATGGG+TGG 0.629419 7.2:+69060602 MS.gene040422:CDS
TGGGCTGAAAGAGAGACAAA+CGG 0.636800 7.2:+69060888 MS.gene040422:CDS
CGAGGGAGGATTCTGATCGG+AGG 0.645667 7.2:-69060317 None:intergenic
AGTGACTGCATCAGATGCGG+TGG 0.649976 7.2:-69061483 None:intergenic
TCTGATGCAGTCACTAGCAG+TGG 0.651539 7.2:+69061491 MS.gene040422:CDS
GGAGAAAAGATGTTTGGCGA+TGG 0.656877 7.2:-69060367 None:intergenic
GGTCGGTCGACAACTTAACT+CGG 0.659941 7.2:+69060937 MS.gene040422:CDS
GAAGGAGTTGAGTTGATCGG+TGG 0.668064 7.2:-69060517 None:intergenic
GCTAGTGACTGCATCAGATG+CGG 0.677841 7.2:-69061486 None:intergenic
TATGCTGAAGGAGCTAGGCG+TGG 0.693931 7.2:+69061337 MS.gene040422:CDS
CCATCGACGAAAGATAGATG+AGG 0.714591 7.2:-69060591 None:intergenic
AAGTTGTCGACCGACCCTAG+AGG 0.749625 7.2:-69060930 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTTTAAATTTTGATA+TGG + chr7.2:69060827-69060846 MS.gene040422:intron 10.0%
!! TACAAAAATTTAACAAAATC+AGG - chr7.2:69060766-69060785 None:intergenic 15.0%
!! CAAATGAAATATTGAACTTT+GGG - chr7.2:69061301-69061320 None:intergenic 20.0%
!!! AAATTTTGATATGGTTTGAA+AGG + chr7.2:69060836-69060855 MS.gene040422:intron 20.0%
! ATCTTATCAAGACAAGAATA+TGG + chr7.2:69060395-69060414 MS.gene040422:CDS 25.0%
! ATTCAAAAGGTGAAAATCAT+AGG - chr7.2:69061033-69061052 None:intergenic 25.0%
! CCAAAGTTCAATATTTCATT+TGG + chr7.2:69061299-69061318 MS.gene040422:CDS 25.0%
! CCAAATGAAATATTGAACTT+TGG - chr7.2:69061302-69061321 None:intergenic 25.0%
! CTCTAATCATAAACAAGAAA+GGG - chr7.2:69061556-69061575 None:intergenic 25.0%
! TCTCTAATCATAAACAAGAA+AGG - chr7.2:69061557-69061576 None:intergenic 25.0%
! TTTATTGACGTAAATGAAGA+AGG + chr7.2:69061443-69061462 MS.gene040422:CDS 25.0%
!! AAGTATTTTGTAGTGAAAAG+AGG - chr7.2:69060735-69060754 None:intergenic 25.0%
!! AGTATTTTGTAGTGAAAAGA+GGG - chr7.2:69060734-69060753 None:intergenic 25.0%
!! TATGATTTTCACCTTTTGAA+TGG + chr7.2:69061032-69061051 MS.gene040422:CDS 25.0%
!!! GAATGATAATTTTGATGCTT+CGG + chr7.2:69060998-69061017 MS.gene040422:CDS 25.0%
!!! GAATGATTTTAGAAATGCAT+AGG - chr7.2:69061415-69061434 None:intergenic 25.0%
!!! TTCGATGATTTTAAAGTTCT+TGG + chr7.2:69061110-69061129 MS.gene040422:CDS 25.0%
ACTGAAACAATCATCTTTGT+TGG + chr7.2:69061584-69061603 MS.gene040422:CDS 30.0%
ATATTGCTTATGGTTCTCTT+CGG - chr7.2:69060258-69060277 None:intergenic 30.0%
ATTAAAGAACTTCTTCCTCT+AGG + chr7.2:69060915-69060934 MS.gene040422:CDS 30.0%
CTTGTCTTGATAAGATTCTT+TGG - chr7.2:69060391-69060410 None:intergenic 30.0%
GACCAATGAAGTGAATTTAT+GGG + chr7.2:69060869-69060888 MS.gene040422:CDS 30.0%
TAACTGAGTTTCCATTCAAA+AGG - chr7.2:69061046-69061065 None:intergenic 30.0%
TGACCAATGAAGTGAATTTA+TGG + chr7.2:69060868-69060887 MS.gene040422:CDS 30.0%
TTAAAGAACTTCTTCCTCTA+GGG + chr7.2:69060916-69060935 MS.gene040422:CDS 30.0%
TTATCTTCACCTTGCTTATA+AGG - chr7.2:69061140-69061159 None:intergenic 30.0%
TTTGAATGGAAACTCAGTTA+AGG + chr7.2:69061046-69061065 MS.gene040422:CDS 30.0%
! GAAAAATGACTTTTGGATCA+AGG - chr7.2:69060236-69060255 None:intergenic 30.0%
! GTATTTTGTAGTGAAAAGAG+GGG - chr7.2:69060733-69060752 None:intergenic 30.0%
! TTCTTTGGAGAAAAGATGTT+TGG - chr7.2:69060376-69060395 None:intergenic 30.0%
!! ATCGATTTGGATATTGCTTA+TGG - chr7.2:69060268-69060287 None:intergenic 30.0%
AACAATCATCTTTGTTGGAC+AGG + chr7.2:69061589-69061608 MS.gene040422:CDS 35.0%
AGCCCATAAATTCACTTCAT+TGG - chr7.2:69060874-69060893 None:intergenic 35.0%
CAATGGAGGTCTTACTAAAA+TGG + chr7.2:69061373-69061392 MS.gene040422:CDS 35.0%
GAAAGAAGGTTGAAGAGAAA+GGG - chr7.2:69060634-69060653 None:intergenic 35.0%
TCCTTCCAAATGCAAAAGAT+GGG + chr7.2:69061183-69061202 MS.gene040422:CDS 35.0%
TCTCTTCAACCTTCTTTCAA+AGG + chr7.2:69060636-69060655 MS.gene040422:CDS 35.0%
TGAAACTTCTGATATGCTGA+AGG + chr7.2:69061325-69061344 MS.gene040422:CDS 35.0%
TGAAAGAAGGTTGAAGAGAA+AGG - chr7.2:69060635-69060654 None:intergenic 35.0%
TTCCTTCCAAATGCAAAAGA+TGG + chr7.2:69061182-69061201 MS.gene040422:CDS 35.0%
! CTCTTCGGAAAAATGACTTT+TGG - chr7.2:69060243-69060262 None:intergenic 35.0%
! GACACAATTCCTTTGAAAGA+AGG - chr7.2:69060648-69060667 None:intergenic 35.0%
! TTGAGAAGTTGTTGTTGTGT+GGG - chr7.2:69060480-69060499 None:intergenic 35.0%
!! CATTTTAGTAAGACCTCCAT+TGG - chr7.2:69061375-69061394 None:intergenic 35.0%
!! TTCTGTCGATTTCAAGAACA+AGG + chr7.2:69060692-69060711 MS.gene040422:CDS 35.0%
AAGTGGAAGTAGGTGACTTT+AGG + chr7.2:69061273-69061292 MS.gene040422:CDS 40.0%
AGAAGCTGTTGATGTTGAGT+GGG - chr7.2:69060291-69060310 None:intergenic 40.0%
AGCACAACTTGTAGTGAAAG+TGG - chr7.2:69060429-69060448 None:intergenic 40.0%
ATCTTTCGTCGATGGTGTAT+GGG + chr7.2:69060599-69060618 MS.gene040422:CDS 40.0%
CAAGCTTCCTCTTGATAAAG+TGG + chr7.2:69061256-69061275 MS.gene040422:CDS 40.0%
CCTACTTCCACTTTATCAAG+AGG - chr7.2:69061266-69061285 None:intergenic 40.0%
CCTCTTGATAAAGTGGAAGT+AGG + chr7.2:69061263-69061282 MS.gene040422:CDS 40.0%
CTTCACCTTGCTTATAAGGA+AGG - chr7.2:69061136-69061155 None:intergenic 40.0%
GAAGAAGCAGAGAACAACTT+TGG - chr7.2:69060184-69060203 None:intergenic 40.0%
GCTAATGCGCTGTACTTTAA+AGG + chr7.2:69060969-69060988 MS.gene040422:CDS 40.0%
GTCTCCGTCATTCTACAAAA+CGG + chr7.2:69060552-69060571 MS.gene040422:CDS 40.0%
GTGTTCCAGTAACTCAGATT+CGG - chr7.2:69061237-69061256 None:intergenic 40.0%
TATCTTTCGTCGATGGTGTA+TGG + chr7.2:69060598-69060617 MS.gene040422:CDS 40.0%
! AGCACCGTTTTGTAGAATGA+CGG - chr7.2:69060559-69060578 None:intergenic 40.0%
! GTTGAGAAGTTGTTGTTGTG+TGG - chr7.2:69060481-69060500 None:intergenic 40.0%
! GTTTTACCTTTCACCAATGG+AGG + chr7.2:69061359-69061378 MS.gene040422:CDS 40.0%
! TGAGAAGTTGTTGTTGTGTG+GGG - chr7.2:69060479-69060498 None:intergenic 40.0%
!! GTGGTTTTACCTTTCACCAA+TGG + chr7.2:69061356-69061375 MS.gene040422:CDS 40.0%
AAAGATGTTTGGCGATGGTG+AGG - chr7.2:69060365-69060384 None:intergenic 45.0%
AAGATGTTTGGCGATGGTGA+GGG - chr7.2:69060364-69060383 None:intergenic 45.0%
CCATCGACGAAAGATAGATG+AGG - chr7.2:69060594-69060613 None:intergenic 45.0%
CCTCATCTATCTTTCGTCGA+TGG + chr7.2:69060591-69060610 MS.gene040422:CDS 45.0%
GAGAAGCTGTTGATGTTGAG+TGG - chr7.2:69060292-69060311 None:intergenic 45.0%
GATGTTGAGTGGGATCGATT+TGG - chr7.2:69060281-69060300 None:intergenic 45.0%
GCTACAAAGTCTAGTCGAGT+TGG - chr7.2:69061527-69061546 None:intergenic 45.0%
GGAGAAAAGATGTTTGGCGA+TGG - chr7.2:69060370-69060389 None:intergenic 45.0%
GTAAGACCTCCATTGGTGAA+AGG - chr7.2:69061368-69061387 None:intergenic 45.0%
GTTGCTATGGTTGTTTGCGA+GGG - chr7.2:69060337-69060356 None:intergenic 45.0%
TCTGATATGCTGAAGGAGCT+AGG + chr7.2:69061332-69061351 MS.gene040422:CDS 45.0%
TGGGCTGAAAGAGAGACAAA+CGG + chr7.2:69060888-69060907 MS.gene040422:CDS 45.0%
! CGCTGTACTTTAAAGGAGAG+TGG + chr7.2:69060976-69060995 MS.gene040422:CDS 45.0%
! CTTGGCCTTCCTTATAAGCA+AGG + chr7.2:69061128-69061147 MS.gene040422:CDS 45.0%
! GCCCATCTTTTGCATTTGGA+AGG - chr7.2:69061187-69061206 None:intergenic 45.0%
! GTTGATCGGTGGATTTGGAT+TGG - chr7.2:69060509-69060528 None:intergenic 45.0%
! TTTGTAGAATGACGGAGACG+AGG - chr7.2:69060551-69060570 None:intergenic 45.0%
!! AGAACTTCTTCCTCTAGGGT+CGG + chr7.2:69060920-69060939 MS.gene040422:CDS 45.0%
!! GTTGAGTTGATCGGTGGATT+TGG - chr7.2:69060514-69060533 None:intergenic 45.0%
AGGTGCTCAATCCTCTTGCT+GGG + chr7.2:69061609-69061628 MS.gene040422:CDS 50.0%
CGTTGCTATGGTTGTTTGCG+AGG - chr7.2:69060338-69060357 None:intergenic 50.0%
CTTCTTCGTGCTCAACATCG+TGG + chr7.2:69060197-69060216 MS.gene040422:CDS 50.0%
GCAGAGAACAACTTTGGCGA+TGG - chr7.2:69060178-69060197 None:intergenic 50.0%
GCCAACTTTGCTTGAGAAGC+TGG + chr7.2:69061208-69061227 MS.gene040422:CDS 50.0%
GCCAGCTTCTCAAGCAAAGT+TGG - chr7.2:69061212-69061231 None:intergenic 50.0%
GCTAGTGACTGCATCAGATG+CGG - chr7.2:69061489-69061508 None:intergenic 50.0%
GGCTTCCGAATCTGAGTTAC+TGG + chr7.2:69061229-69061248 MS.gene040422:CDS 50.0%
GGTCGGTCGACAACTTAACT+CGG + chr7.2:69060937-69060956 MS.gene040422:CDS 50.0%
TCTGATGCAGTCACTAGCAG+TGG + chr7.2:69061491-69061510 MS.gene040422:CDS 50.0%
TGGTGAGGGAAACGTTGCTA+TGG - chr7.2:69060350-69060369 None:intergenic 50.0%
TTGCGAGGGAGGATTCTGAT+CGG - chr7.2:69060323-69060342 None:intergenic 50.0%
TTTCGTCGATGGTGTATGGG+TGG + chr7.2:69060602-69060621 MS.gene040422:CDS 50.0%
! AATGACGGAGACGAGGATAG+AGG - chr7.2:69060544-69060563 None:intergenic 50.0%
! GATCGGTGGATTTGGATTGG+AGG - chr7.2:69060506-69060525 None:intergenic 50.0%
! GGCAGCCCATCTTTTGCATT+TGG - chr7.2:69061191-69061210 None:intergenic 50.0%
!! GAAGGAGTTGAGTTGATCGG+TGG - chr7.2:69060520-69060539 None:intergenic 50.0%
!! GGCGAAGGAGTTGAGTTGAT+CGG - chr7.2:69060523-69060542 None:intergenic 50.0%
AAGTTGTCGACCGACCCTAG+AGG - chr7.2:69060933-69060952 None:intergenic 55.0%
AGTGACTGCATCAGATGCGG+TGG - chr7.2:69061486-69061505 None:intergenic 55.0%
CAGGTGCTCAATCCTCTTGC+TGG + chr7.2:69061608-69061627 MS.gene040422:CDS 55.0%
GCAGTCACTAGCAGTGGCAT+GGG + chr7.2:69061497-69061516 MS.gene040422:CDS 55.0%
GCTATGGTTGTTTGCGAGGG+AGG - chr7.2:69060334-69060353 None:intergenic 55.0%
GTGCTAAGCATCATCGCTGC+TGG + chr7.2:69060444-69060463 MS.gene040422:CDS 55.0%
TATGCTGAAGGAGCTAGGCG+TGG + chr7.2:69061337-69061356 MS.gene040422:CDS 55.0%
TGCAGTCACTAGCAGTGGCA+TGG + chr7.2:69061496-69061515 MS.gene040422:CDS 55.0%
! TTGTTGTTGTGTGGGGCCCT+CGG - chr7.2:69060472-69060491 None:intergenic 55.0%
AGATAGATGAGGCCCGCCTG+CGG - chr7.2:69060583-69060602 None:intergenic 60.0%
CGAGGGAGGATTCTGATCGG+AGG - chr7.2:69060320-69060339 None:intergenic 60.0%
CTACAAAACGGTGCTCCCGC+AGG + chr7.2:69060564-69060583 MS.gene040422:CDS 60.0%
GGAGACGAGGATAGAGGCGA+AGG - chr7.2:69060538-69060557 None:intergenic 60.0%
TGCGGTGGCTGCAACAGCTT+CGG - chr7.2:69061471-69061490 None:intergenic 60.0%
!! ATCATCGCTGCTGGCTCCGA+GGG + chr7.2:69060453-69060472 MS.gene040422:CDS 60.0%
AAAACGGTGCTCCCGCAGGC+GGG + chr7.2:69060568-69060587 MS.gene040422:CDS 65.0%
CAAAACGGTGCTCCCGCAGG+CGG + chr7.2:69060567-69060586 MS.gene040422:CDS 65.0%
GATAGATGAGGCCCGCCTGC+GGG - chr7.2:69060582-69060601 None:intergenic 65.0%
!! CATCATCGCTGCTGGCTCCG+AGG + chr7.2:69060452-69060471 MS.gene040422:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 69060150 69061634 69060150 ID=MS.gene040422
chr7.2 mRNA 69060150 69061634 69060150 ID=MS.gene040422.t1;Parent=MS.gene040422
chr7.2 exon 69060150 69060713 69060150 ID=MS.gene040422.t1.exon1;Parent=MS.gene040422.t1
chr7.2 CDS 69060150 69060713 69060150 ID=cds.MS.gene040422.t1;Parent=MS.gene040422.t1
chr7.2 exon 69060858 69061634 69060858 ID=MS.gene040422.t1.exon2;Parent=MS.gene040422.t1
chr7.2 CDS 69060858 69061634 69060858 ID=cds.MS.gene040422.t1;Parent=MS.gene040422.t1
Gene Sequence

>MS.gene040422

ATGAGGTTCTCTCGCAAAACAATCGCCATCGCCAAAGTTGTTCTCTGCTTCTTCGTGCTCAACATCGTGGCTTGTTTAATCATCCTTGATCCAAAAGTCATTTTTCCGAAGAGAACCATAAGCAATATCCAAATCGATCCCACTCAACATCAACAGCTTCTCGACTTCCTCCGATCAGAATCCTCCCTCGCAAACAACCATAGCAACGTTTCCCTCACCATCGCCAAACATCTTTTCTCCAAAGAATCTTATCAAGACAAGAATATGGTGTTATCTCCACTTTCACTACAAGTTGTGCTAAGCATCATCGCTGCTGGCTCCGAGGGCCCCACACAACAACAACTTCTCAACTTCCTCCAATCCAAATCCACCGATCAACTCAACTCCTTCGCCTCTATCCTCGTCTCCGTCATTCTACAAAACGGTGCTCCCGCAGGCGGGCCTCATCTATCTTTCGTCGATGGTGTATGGGTGGAAAAAACCCTTTCTCTTCAACCTTCTTTCAAAGGAATTGTGTCTAATGATTATAAAGCAAATTTGTCTTCTGTCGATTTCAAGAACAAGGTATGTTTCTATATCTCCCCTCTTTTCACTACAAAATACTTATGCATGTCCTGATTTTGTTAAATTTTTGTATTGCAATACTTGTGAAAAGAACTCAACTATATATTTGCATAATTGATTTTAAATTTTGATATGGTTTGAAAGGCTATTGAAGTGACCAATGAAGTGAATTTATGGGCTGAAAGAGAGACAAACGGTCTTATTAAAGAACTTCTTCCTCTAGGGTCGGTCGACAACTTAACTCGGCTAATATTTGCTAATGCGCTGTACTTTAAAGGAGAGTGGAATGATAATTTTGATGCTTCGGATACGAATGCCTATGATTTTCACCTTTTGAATGGAAACTCAGTTAAGGTTGACTTCATGACTAGTGAGAAGAAGCAGTTTATTAGAGATTTCGATGATTTTAAAGTTCTTGGCCTTCCTTATAAGCAAGGTGAAGATAAGCGTCAATTTACTATGTACTTTTTCCTTCCAAATGCAAAAGATGGGCTGCCAACTTTGCTTGAGAAGCTGGCTTCCGAATCTGAGTTACTGGAACACAAGCTTCCTCTTGATAAAGTGGAAGTAGGTGACTTTAGGATCCCAAAGTTCAATATTTCATTTGGACTTGAAACTTCTGATATGCTGAAGGAGCTAGGCGTGGTTTTACCTTTCACCAATGGAGGTCTTACTAAAATGGTGAACTCTACTCAAAACCTATGCATTTCTAAAATCATTCACAAGTCTTTTATTGACGTAAATGAAGAAGGCACCGAAGCTGTTGCAGCCACCGCATCTGATGCAGTCACTAGCAGTGGCATGGGTTTTCCAACTCGACTAGACTTTGTAGCTGATCACCCTTTCTTGTTTATGATTAGAGAAGAATTGACTGAAACAATCATCTTTGTTGGACAGGTGCTCAATCCTCTTGCTGGGTGA
Protein sequence

>MS.gene040422.t1

MRFSRKTIAIAKVVLCFFVLNIVACLIILDPKVIFPKRTISNIQIDPTQHQQLLDFLRSESSLANNHSNVSLTIAKHLFSKESYQDKNMVLSPLSLQVVLSIIAAGSEGPTQQQLLNFLQSKSTDQLNSFASILVSVILQNGAPAGGPHLSFVDGVWVEKTLSLQPSFKGIVSNDYKANLSSVDFKNKAIEVTNEVNLWAERETNGLIKELLPLGSVDNLTRLIFANALYFKGEWNDNFDASDTNAYDFHLLNGNSVKVDFMTSEKKQFIRDFDDFKVLGLPYKQGEDKRQFTMYFFLPNAKDGLPTLLEKLASESELLEHKLPLDKVEVGDFRIPKFNISFGLETSDMLKELGVVLPFTNGGLTKMVNSTQNLCISKIIHKSFIDVNEEGTEAVAATASDAVTSSGMGFPTRLDFVADHPFLFMIREELTETIIFVGQVLNPLAG