Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040430.t1 | RHN70239.1 | 77.7 | 439 | 74 | 5 | 28 | 443 | 1 | 438 | 4.20E-180 | 641 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040430.t1 | Q9S7T8 | 52.6 | 392 | 175 | 5 | 57 | 441 | 3 | 390 | 3.5e-111 | 403.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040430.t1 | A0A396IX06 | 77.7 | 439 | 74 | 5 | 28 | 443 | 1 | 438 | 3.0e-180 | 641.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene031697 | MS.gene040430 | PPI |
| MS.gene040430 | MS.gene065944 | PPI |
| MS.gene065945 | MS.gene040430 | PPI |
| MS.gene040430 | MS.gene045585 | PPI |
| MS.gene006610 | MS.gene040430 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040430.t1 | MTR_3g101030 | 79.059 | 425 | 69 | 4 | 39 | 443 | 5 | 429 | 0.0 | 662 |
| MS.gene040430.t1 | MTR_3g101050 | 72.707 | 447 | 67 | 7 | 1 | 442 | 1 | 397 | 0.0 | 602 |
| MS.gene040430.t1 | MTR_3g101010 | 80.157 | 383 | 69 | 3 | 66 | 443 | 9 | 389 | 0.0 | 590 |
| MS.gene040430.t1 | MTR_3g018740 | 69.816 | 381 | 109 | 3 | 65 | 441 | 8 | 386 | 0.0 | 517 |
| MS.gene040430.t1 | MTR_8g027395 | 66.499 | 397 | 125 | 4 | 49 | 443 | 7 | 397 | 0.0 | 516 |
| MS.gene040430.t1 | MTR_4g045707 | 67.179 | 390 | 121 | 4 | 57 | 443 | 3 | 388 | 4.93e-180 | 508 |
| MS.gene040430.t1 | MTR_8g461160 | 65.306 | 392 | 129 | 2 | 57 | 441 | 1 | 392 | 2.09e-177 | 501 |
| MS.gene040430.t1 | MTR_8g027420 | 66.230 | 382 | 113 | 4 | 64 | 443 | 8 | 375 | 3.19e-174 | 493 |
| MS.gene040430.t1 | MTR_3g101180 | 64.573 | 398 | 126 | 5 | 57 | 441 | 3 | 398 | 1.27e-172 | 489 |
| MS.gene040430.t1 | MTR_7g451890 | 62.338 | 385 | 138 | 2 | 66 | 443 | 18 | 402 | 8.97e-168 | 477 |
| MS.gene040430.t1 | MTR_3g048000 | 63.117 | 385 | 120 | 4 | 60 | 441 | 25 | 390 | 2.88e-167 | 476 |
| MS.gene040430.t1 | MTR_4g088525 | 65.385 | 364 | 121 | 3 | 81 | 442 | 8 | 368 | 4.30e-165 | 469 |
| MS.gene040430.t1 | MTR_7g050810 | 61.917 | 386 | 138 | 3 | 66 | 443 | 20 | 404 | 5.24e-165 | 470 |
| MS.gene040430.t1 | MTR_7g047780 | 61.818 | 385 | 140 | 2 | 66 | 443 | 18 | 402 | 8.33e-165 | 470 |
| MS.gene040430.t1 | MTR_4g088535 | 64.752 | 383 | 112 | 6 | 58 | 438 | 5 | 366 | 1.14e-163 | 466 |
| MS.gene040430.t1 | MTR_7g050750 | 61.299 | 385 | 142 | 2 | 66 | 443 | 12 | 396 | 1.23e-162 | 464 |
| MS.gene040430.t1 | MTR_2g046030 | 63.185 | 383 | 129 | 5 | 66 | 441 | 9 | 386 | 3.77e-162 | 462 |
| MS.gene040430.t1 | MTR_3g101130 | 60.881 | 386 | 144 | 3 | 62 | 443 | 7 | 389 | 7.89e-161 | 459 |
| MS.gene040430.t1 | MTR_2g045980 | 62.924 | 383 | 130 | 5 | 66 | 441 | 9 | 386 | 8.10e-161 | 459 |
| MS.gene040430.t1 | MTR_3g015620 | 62.141 | 383 | 114 | 5 | 66 | 441 | 20 | 378 | 3.74e-151 | 434 |
| MS.gene040430.t1 | MTR_3g100995 | 75.591 | 254 | 60 | 1 | 192 | 443 | 11 | 264 | 1.86e-139 | 400 |
| MS.gene040430.t1 | MTR_3g102920 | 58.047 | 379 | 116 | 4 | 70 | 441 | 66 | 408 | 1.42e-138 | 403 |
| MS.gene040430.t1 | MTR_3g101200 | 54.743 | 369 | 139 | 4 | 78 | 441 | 45 | 390 | 7.25e-126 | 370 |
| MS.gene040430.t1 | MTR_3g101190 | 54.372 | 366 | 101 | 5 | 83 | 443 | 8 | 312 | 1.72e-123 | 361 |
| MS.gene040430.t1 | MTR_3g018747 | 68.340 | 259 | 63 | 3 | 188 | 441 | 9 | 253 | 7.46e-119 | 347 |
| MS.gene040430.t1 | MTR_3g100010 | 57.764 | 322 | 88 | 2 | 85 | 373 | 38 | 344 | 1.72e-115 | 342 |
| MS.gene040430.t1 | MTR_7g050830 | 55.016 | 309 | 108 | 2 | 68 | 356 | 22 | 319 | 3.52e-111 | 330 |
| MS.gene040430.t1 | MTR_3g015760 | 60.507 | 276 | 98 | 4 | 173 | 441 | 8 | 279 | 4.47e-99 | 298 |
| MS.gene040430.t1 | MTR_3g111160 | 67.943 | 209 | 61 | 3 | 240 | 443 | 13 | 220 | 5.71e-88 | 267 |
| MS.gene040430.t1 | MTR_3g100520 | 61.644 | 219 | 70 | 3 | 229 | 443 | 1 | 209 | 8.18e-87 | 264 |
| MS.gene040430.t1 | MTR_3g114310 | 42.317 | 397 | 205 | 11 | 64 | 443 | 21 | 410 | 3.16e-86 | 270 |
| MS.gene040430.t1 | MTR_3g101110 | 60.251 | 239 | 60 | 4 | 188 | 417 | 68 | 280 | 5.24e-86 | 265 |
| MS.gene040430.t1 | MTR_3g099970 | 65.641 | 195 | 50 | 2 | 188 | 381 | 20 | 198 | 4.06e-82 | 251 |
| MS.gene040430.t1 | MTR_2g046410 | 63.366 | 202 | 65 | 4 | 248 | 441 | 147 | 347 | 2.05e-77 | 244 |
| MS.gene040430.t1 | MTR_3g101020 | 66.848 | 184 | 35 | 2 | 262 | 443 | 1 | 160 | 2.20e-77 | 238 |
| MS.gene040430.t1 | MTR_3g111150 | 58.757 | 177 | 47 | 2 | 66 | 242 | 3 | 153 | 2.69e-61 | 197 |
| MS.gene040430.t1 | MTR_3g101150 | 61.146 | 157 | 52 | 2 | 294 | 441 | 1 | 157 | 1.68e-58 | 189 |
| MS.gene040430.t1 | MTR_3g102870 | 59.211 | 152 | 60 | 1 | 251 | 400 | 1 | 152 | 2.37e-55 | 182 |
| MS.gene040430.t1 | MTR_3g100990 | 58.108 | 148 | 59 | 2 | 66 | 213 | 9 | 153 | 6.54e-44 | 160 |
| MS.gene040430.t1 | MTR_4g093550 | 62.195 | 82 | 29 | 1 | 66 | 147 | 10 | 89 | 5.29e-24 | 95.9 |
| MS.gene040430.t1 | MTR_6g046890 | 64.474 | 76 | 24 | 1 | 80 | 155 | 15 | 87 | 7.37e-20 | 85.9 |
| MS.gene040430.t1 | MTR_3g101140 | 61.538 | 65 | 21 | 1 | 383 | 443 | 52 | 116 | 2.44e-17 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040430.t1 | AT1G47710 | 51.852 | 405 | 183 | 6 | 44 | 441 | 18 | 417 | 7.03e-142 | 412 |
| MS.gene040430.t1 | AT1G47710 | 52.551 | 392 | 175 | 5 | 57 | 441 | 3 | 390 | 9.85e-142 | 411 |
| MS.gene040430.t1 | AT3G45220 | 48.205 | 390 | 179 | 7 | 66 | 440 | 9 | 390 | 2.69e-121 | 359 |
| MS.gene040430.t1 | AT2G25240 | 47.244 | 381 | 188 | 6 | 66 | 440 | 9 | 382 | 2.65e-115 | 343 |
| MS.gene040430.t1 | AT2G26390 | 46.094 | 384 | 192 | 5 | 66 | 440 | 9 | 386 | 1.07e-112 | 337 |
| MS.gene040430.t1 | AT1G62170 | 42.623 | 366 | 182 | 4 | 85 | 442 | 88 | 433 | 5.66e-96 | 295 |
| MS.gene040430.t1 | AT1G64030 | 40.682 | 381 | 202 | 6 | 66 | 440 | 9 | 371 | 2.94e-92 | 284 |
| MS.gene040430.t1 | AT2G14540 | 41.053 | 380 | 200 | 5 | 66 | 440 | 37 | 397 | 5.80e-92 | 284 |
| MS.gene040430.t1 | AT1G62170 | 39.098 | 399 | 182 | 5 | 85 | 442 | 88 | 466 | 3.85e-89 | 279 |
| MS.gene040430.t1 | AT2G35580 | 41.398 | 372 | 188 | 8 | 81 | 440 | 18 | 371 | 1.28e-88 | 275 |
| MS.gene040430.t1 | AT1G64010 | 45.794 | 214 | 95 | 4 | 229 | 440 | 1 | 195 | 1.13e-57 | 189 |
| MS.gene040430.t1 | AT1G64010 | 45.729 | 199 | 87 | 4 | 244 | 440 | 2 | 181 | 8.74e-52 | 173 |
| MS.gene040430.t1 | AT1G62160 | 40.782 | 179 | 86 | 4 | 266 | 442 | 60 | 220 | 5.36e-36 | 132 |
| MS.gene040430.t1 | AT1G63280 | 52.174 | 115 | 51 | 2 | 188 | 300 | 8 | 120 | 1.44e-31 | 117 |
| MS.gene040430.t1 | AT1G51330 | 49.550 | 111 | 53 | 2 | 187 | 294 | 30 | 140 | 2.24e-23 | 97.4 |
| MS.gene040430.t1 | AT1G64020 | 33.333 | 111 | 71 | 3 | 70 | 179 | 13 | 121 | 9.72e-13 | 65.1 |
Find 106 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAATGCGCTGTACTTTAA+AGG | 0.166787 | 7.4:-72315555 | MS.gene040430:CDS |
| TGACCAATGAAGTGAATTTA+TGG | 0.210594 | 7.4:-72315656 | MS.gene040430:CDS |
| TTATCTTCACCTTGCTTATA+AGG | 0.268250 | 7.4:+72315387 | None:intergenic |
| CTTGTCTTGATAAGATTCTT+TGG | 0.269178 | 7.4:+72316136 | None:intergenic |
| CAATGGAGGTCTTACTAAAA+TGG | 0.273021 | 7.4:-72315151 | MS.gene040430:CDS |
| CGCATCTGATGCAGTCAGTA+TGG | 0.276049 | 7.4:-72315037 | MS.gene040430:CDS |
| ATCGATTTGGATATTGCTTA+TGG | 0.285955 | 7.4:+72316259 | None:intergenic |
| CCCAACTTTGCTTGAGAAGC+TGG | 0.289017 | 7.4:-72315316 | MS.gene040430:CDS |
| CCAAAGTTCAATATTTCATT+TGG | 0.296966 | 7.4:-72315225 | MS.gene040430:CDS |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.306550 | 7.4:-72314925 | MS.gene040430:CDS |
| GTTGAGTTGATCGGTGGATT+TGG | 0.319346 | 7.4:+72316013 | None:intergenic |
| CAAATGAAATATTGAACTTT+GGG | 0.323605 | 7.4:+72315226 | None:intergenic |
| GGCTTCCGAATCTGAGTTAC+TGG | 0.338294 | 7.4:-72315295 | MS.gene040430:CDS |
| AGCCCATAAATTCACTTCAT+TGG | 0.342189 | 7.4:+72315653 | None:intergenic |
| TATCTTTCGTCGATGGTGTA+TGG | 0.345495 | 7.4:-72315926 | MS.gene040430:CDS |
| GTGCTAAGCATCATCGCTTC+TGG | 0.361357 | 7.4:-72316080 | MS.gene040430:CDS |
| AAGTGGAAGTAGGTGACTTT+AGG | 0.367192 | 7.4:-72315251 | MS.gene040430:CDS |
| CCAAATGAAATATTGAACTT+TGG | 0.376715 | 7.4:+72315225 | None:intergenic |
| TTCTTTGGAGAAAAGATGTT+TGG | 0.376879 | 7.4:+72316151 | None:intergenic |
| GGGAGCCCATCTTGTGCATT+TGG | 0.377583 | 7.4:+72315336 | None:intergenic |
| TGCGGTGGCTGCAACAGCTT+CGG | 0.384988 | 7.4:+72315056 | None:intergenic |
| ATTCAAAAGGCGAAAATCAT+AGG | 0.386713 | 7.4:+72315494 | None:intergenic |
| ACTGGAACAATCATCTTTGT+TGG | 0.397026 | 7.4:-72314949 | MS.gene040430:CDS |
| GAAAGAAGGTTGAAGAGAAA+GGG | 0.417612 | 7.4:+72315893 | None:intergenic |
| TGAAAGAAGGTTGAAGAGAA+AGG | 0.419511 | 7.4:+72315892 | None:intergenic |
| ATCTTATCAAGACAAGAATA+TGG | 0.421750 | 7.4:-72316129 | MS.gene040430:CDS |
| GACACAATTCCTTTGAAAGA+AGG | 0.426001 | 7.4:+72315879 | None:intergenic |
| TTCCTTCCAAATGCACAAGA+TGG | 0.428926 | 7.4:-72315342 | MS.gene040430:CDS |
| GCCAGCTTCTCAAGCAAAGT+TGG | 0.437737 | 7.4:+72315315 | None:intergenic |
| AACAATCATCTTTGTTGGAC+AGG | 0.442686 | 7.4:-72314944 | MS.gene040430:CDS |
| TCTCTAATCATAAACAAGAA+AGG | 0.443816 | 7.4:+72314979 | None:intergenic |
| TTTGAATGGAAACTCAGTTA+AGG | 0.446012 | 7.4:-72315478 | MS.gene040430:CDS |
| CAAGCTTCCTCTTGATAAAG+TGG | 0.447424 | 7.4:-72315268 | MS.gene040430:CDS |
| GATGTTGAGTGGGATCGATT+TGG | 0.447564 | 7.4:+72316246 | None:intergenic |
| TTTATTGACGTAAATGAAGA+AGG | 0.449783 | 7.4:-72315081 | MS.gene040430:CDS |
| TAACTGAGTTTCCATTCAAA+AGG | 0.453776 | 7.4:+72315481 | None:intergenic |
| CTCTAATCATAAACAAGAAA+GGG | 0.468439 | 7.4:+72314980 | None:intergenic |
| TCAAAGAACTTCTTCCTCTA+GGG | 0.473973 | 7.4:-72315608 | MS.gene040430:CDS |
| TCTCTTCAACCTTCTTTCAA+AGG | 0.474212 | 7.4:-72315888 | MS.gene040430:CDS |
| GTGTTCCAGTAACTCAGATT+CGG | 0.474362 | 7.4:+72315290 | None:intergenic |
| ATCAAAGAACTTCTTCCTCT+AGG | 0.484467 | 7.4:-72315609 | MS.gene040430:CDS |
| ATGATTAGAGAAGAATTGAC+TGG | 0.486911 | 7.4:-72314967 | MS.gene040430:CDS |
| ATCTTTCGTCGATGGTGTAT+GGG | 0.491547 | 7.4:-72315925 | MS.gene040430:CDS |
| GATAGATGAGGCCCGCCTGC+GGG | 0.493469 | 7.4:+72315945 | None:intergenic |
| TTGAGAAGTTGTTGTTGTGT+GGG | 0.494908 | 7.4:+72316047 | None:intergenic |
| GACCAATGAAGTGAATTTAT+GGG | 0.495263 | 7.4:-72315655 | MS.gene040430:CDS |
| GTTGATCGGTGGATTTGGAT+TGG | 0.496620 | 7.4:+72316018 | None:intergenic |
| TCCTTCCAAATGCACAAGAT+GGG | 0.496874 | 7.4:-72315341 | MS.gene040430:CDS |
| CTTCACCTTGCTTATAAGGA+AGG | 0.497179 | 7.4:+72315391 | None:intergenic |
| ATATTGCTTATGGTTCTCTT+CGG | 0.500550 | 7.4:+72316269 | None:intergenic |
| AGGTGCTCAATCCTCTTGCT+GGG | 0.503743 | 7.4:-72314924 | MS.gene040430:CDS |
| TTGTTGTTGTGTGGGGCCCT+CGG | 0.506763 | 7.4:+72316055 | None:intergenic |
| CCAGCTTCTCAAGCAAAGTT+GGG | 0.508851 | 7.4:+72315316 | None:intergenic |
| GTTGAGAAGTTGTTGTTGTG+TGG | 0.516014 | 7.4:+72316046 | None:intergenic |
| GCTATGGTTGTTTGCGAGGG+AGG | 0.521590 | 7.4:+72316193 | None:intergenic |
| AAAGATGTTTGGCGATGGTG+AGG | 0.524270 | 7.4:+72316162 | None:intergenic |
| ATCATCGCTTCTGGCTCCGA+GGG | 0.524555 | 7.4:-72316071 | MS.gene040430:CDS |
| AGCACAACTTGTAGTGAAAG+TGG | 0.525473 | 7.4:+72316098 | None:intergenic |
| GCCCATCTTGTGCATTTGGA+AGG | 0.526536 | 7.4:+72315340 | None:intergenic |
| CTTGGCCTTCCTTATAAGCA+AGG | 0.532934 | 7.4:-72315396 | MS.gene040430:CDS |
| CGCTGTACTTTAAAGGAGAG+TGG | 0.534492 | 7.4:-72315548 | MS.gene040430:CDS |
| GATCGGTGGATTTGGATTGG+AGG | 0.535140 | 7.4:+72316021 | None:intergenic |
| AATGACGGAGACGAGGATAG+AGG | 0.536198 | 7.4:+72315983 | None:intergenic |
| GGAGACGAGGATAGAGGCGA+AGG | 0.536431 | 7.4:+72315989 | None:intergenic |
| TCTGATATGCTGAAGGAGCT+AGG | 0.542423 | 7.4:-72315192 | MS.gene040430:CDS |
| GAAGAAGCAGAGAACAACTT+TGG | 0.549721 | 7.4:+72316343 | None:intergenic |
| AAAACGGTGCTCCCGCAGGC+GGG | 0.550168 | 7.4:-72315956 | MS.gene040430:CDS |
| CTACAAAACGGTGCTCCCGC+AGG | 0.550410 | 7.4:-72315960 | MS.gene040430:CDS |
| CGTTGCTATGGTTGTTTGCG+AGG | 0.559095 | 7.4:+72316189 | None:intergenic |
| CCTACTTCCACTTTATCAAG+AGG | 0.564451 | 7.4:+72315261 | None:intergenic |
| GCTACAAAGTCTAGTCGAGT+TGG | 0.564663 | 7.4:+72315009 | None:intergenic |
| GAGAAGCTGTTGATGTTGAG+TGG | 0.565808 | 7.4:+72316235 | None:intergenic |
| CCTCATCTATCTTTCGTCGA+TGG | 0.565945 | 7.4:-72315933 | MS.gene040430:CDS |
| CCTCTTGATAAAGTGGAAGT+AGG | 0.566916 | 7.4:-72315261 | MS.gene040430:CDS |
| TTCTGTCGATTTCAAGAACA+AGG | 0.572001 | 7.4:-72315832 | MS.gene040430:intron |
| GTCTCCGTCATTCTACAAAA+CGG | 0.572969 | 7.4:-72315972 | MS.gene040430:CDS |
| GCATCTGATGCAGTCAGTAT+GGG | 0.576573 | 7.4:-72315036 | MS.gene040430:CDS |
| GTTGCTATGGTTGTTTGCGA+GGG | 0.577691 | 7.4:+72316190 | None:intergenic |
| TGAGAAGTTGTTGTTGTGTG+GGG | 0.577961 | 7.4:+72316048 | None:intergenic |
| GTAAGACCTCCATTGGTGAA+AGG | 0.579413 | 7.4:+72315159 | None:intergenic |
| CACTAAAATCACCCAGCAAG+AGG | 0.587700 | 7.4:+72314913 | None:intergenic |
| CAAAACGGTGCTCCCGCAGG+CGG | 0.589050 | 7.4:-72315957 | MS.gene040430:CDS |
| TTTGTAGAATGACGGAGACG+AGG | 0.590002 | 7.4:+72315976 | None:intergenic |
| CATCATCGCTTCTGGCTCCG+AGG | 0.591530 | 7.4:-72316072 | MS.gene040430:CDS |
| AAGATGTTTGGCGATGGTGA+GGG | 0.596429 | 7.4:+72316163 | None:intergenic |
| GCAGAGAACAACTTTGGCGA+TGG | 0.601250 | 7.4:+72316349 | None:intergenic |
| GGCGAAGGAGTTGAGTTGAT+CGG | 0.602516 | 7.4:+72316004 | None:intergenic |
| TGAAACTTCTGATATGCTGA+AGG | 0.602682 | 7.4:-72315199 | MS.gene040430:CDS |
| AGATAGATGAGGCCCGCCTG+CGG | 0.604970 | 7.4:+72315944 | None:intergenic |
| TGGTGAGGGAAACGTTGCTA+TGG | 0.613033 | 7.4:+72316177 | None:intergenic |
| TTGCGAGGGAGGATTCTGAT+CGG | 0.616661 | 7.4:+72316204 | None:intergenic |
| CTTCTTCGTGCTCAACATCG+TGG | 0.619017 | 7.4:-72316327 | MS.gene040430:CDS |
| AGAAGCTGTTGATGTTGAGT+GGG | 0.619685 | 7.4:+72316236 | None:intergenic |
| TTTCGTCGATGGTGTATGGG+TGG | 0.629419 | 7.4:-72315922 | MS.gene040430:CDS |
| TGGGCTGAAAGAGAGACAAA+CGG | 0.636800 | 7.4:-72315636 | MS.gene040430:CDS |
| ACTGACTGCATCAGATGCGG+TGG | 0.645350 | 7.4:+72315041 | None:intergenic |
| CGAGGGAGGATTCTGATCGG+AGG | 0.645667 | 7.4:+72316207 | None:intergenic |
| AGAACTTCTTCCTCTAGGGT+CGG | 0.649781 | 7.4:-72315604 | MS.gene040430:CDS |
| GGAGAAAAGATGTTTGGCGA+TGG | 0.656877 | 7.4:+72316157 | None:intergenic |
| GGTCGGTCGACAACTTAACT+CGG | 0.659941 | 7.4:-72315587 | MS.gene040430:CDS |
| GAAGGAGTTGAGTTGATCGG+TGG | 0.668064 | 7.4:+72316007 | None:intergenic |
| CATACTGACTGCATCAGATG+CGG | 0.683885 | 7.4:+72315038 | None:intergenic |
| TATGCTGAAGGAGCTAGGCG+TGG | 0.693931 | 7.4:-72315187 | MS.gene040430:CDS |
| AACTCGACTAGACTTTGTAG+CGG | 0.708512 | 7.4:-72315007 | MS.gene040430:CDS |
| CCATCGACGAAAGATAGATG+AGG | 0.714591 | 7.4:+72315933 | None:intergenic |
| AAGTTGTCGACCGACCCTAG+AGG | 0.749625 | 7.4:+72315594 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATTTTAAATTTTGATA+TGG | - | chr7.4:72315598-72315617 | MS.gene040430:CDS | 10.0% |
| !! | TACAAAAATTTAACAAAATC+AGG | + | chr7.4:72315537-72315556 | None:intergenic | 15.0% |
| !! | CAAATGAAATATTGAACTTT+GGG | + | chr7.4:72316072-72316091 | None:intergenic | 20.0% |
| !!! | AAATTTTGATATGGTTTGAA+AGG | - | chr7.4:72315607-72315626 | MS.gene040430:CDS | 20.0% |
| !!! | GAATAATAATTTTGATGCTT+CGG | - | chr7.4:72315769-72315788 | MS.gene040430:intron | 20.0% |
| ! | ATCTTATCAAGACAAGAATA+TGG | - | chr7.4:72315166-72315185 | MS.gene040430:CDS | 25.0% |
| ! | CCAAAGTTCAATATTTCATT+TGG | - | chr7.4:72316070-72316089 | MS.gene040430:CDS | 25.0% |
| ! | CCAAATGAAATATTGAACTT+TGG | + | chr7.4:72316073-72316092 | None:intergenic | 25.0% |
| ! | CTCTAATCATAAACAAGAAA+GGG | + | chr7.4:72316318-72316337 | None:intergenic | 25.0% |
| ! | TCTCTAATCATAAACAAGAA+AGG | + | chr7.4:72316319-72316338 | None:intergenic | 25.0% |
| ! | TTTATTGACGTAAATGAAGA+AGG | - | chr7.4:72316214-72316233 | MS.gene040430:CDS | 25.0% |
| !! | AAGTATTTTGTAGTGAAAAG+AGG | + | chr7.4:72315506-72315525 | None:intergenic | 25.0% |
| !! | AGTATTTTGTAGTGAAAAGA+GGG | + | chr7.4:72315505-72315524 | None:intergenic | 25.0% |
| !!! | GAATGATTTTAGAAATGCAT+AGG | + | chr7.4:72316186-72316205 | None:intergenic | 25.0% |
| !!! | TTCGATGATTTTAAAGTTCT+TGG | - | chr7.4:72315881-72315900 | MS.gene040430:CDS | 25.0% |
| ATATTGCTTATGGTTCTCTT+CGG | + | chr7.4:72315029-72315048 | None:intergenic | 30.0% | |
| ATGATTAGAGAAGAATTGAC+TGG | - | chr7.4:72316328-72316347 | MS.gene040430:CDS | 30.0% | |
| ATTCAAAAGGCGAAAATCAT+AGG | + | chr7.4:72315804-72315823 | None:intergenic | 30.0% | |
| CTTGTCTTGATAAGATTCTT+TGG | + | chr7.4:72315162-72315181 | None:intergenic | 30.0% | |
| GACCAATGAAGTGAATTTAT+GGG | - | chr7.4:72315640-72315659 | MS.gene040430:CDS | 30.0% | |
| TAACTGAGTTTCCATTCAAA+AGG | + | chr7.4:72315817-72315836 | None:intergenic | 30.0% | |
| TGACCAATGAAGTGAATTTA+TGG | - | chr7.4:72315639-72315658 | MS.gene040430:CDS | 30.0% | |
| TTATCTTCACCTTGCTTATA+AGG | + | chr7.4:72315911-72315930 | None:intergenic | 30.0% | |
| TTTGAATGGAAACTCAGTTA+AGG | - | chr7.4:72315817-72315836 | MS.gene040430:intron | 30.0% | |
| ! | GAAAAATGACTTTTGGATCA+AGG | + | chr7.4:72315007-72315026 | None:intergenic | 30.0% |
| ! | GTATTTTGTAGTGAAAAGAG+GGG | + | chr7.4:72315504-72315523 | None:intergenic | 30.0% |
| ! | TATGATTTTCGCCTTTTGAA+TGG | - | chr7.4:72315803-72315822 | MS.gene040430:intron | 30.0% |
| ! | TTCTTTGGAGAAAAGATGTT+TGG | + | chr7.4:72315147-72315166 | None:intergenic | 30.0% |
| !! | ATCGATTTGGATATTGCTTA+TGG | + | chr7.4:72315039-72315058 | None:intergenic | 30.0% |
| AACAATCATCTTTGTTGGAC+AGG | - | chr7.4:72316351-72316370 | MS.gene040430:CDS | 35.0% | |
| ACTGGAACAATCATCTTTGT+TGG | - | chr7.4:72316346-72316365 | MS.gene040430:CDS | 35.0% | |
| AGCCCATAAATTCACTTCAT+TGG | + | chr7.4:72315645-72315664 | None:intergenic | 35.0% | |
| ATCAAAGAACTTCTTCCTCT+AGG | - | chr7.4:72315686-72315705 | MS.gene040430:intron | 35.0% | |
| CAATGGAGGTCTTACTAAAA+TGG | - | chr7.4:72316144-72316163 | MS.gene040430:CDS | 35.0% | |
| GAAAGAAGGTTGAAGAGAAA+GGG | + | chr7.4:72315405-72315424 | None:intergenic | 35.0% | |
| TCAAAGAACTTCTTCCTCTA+GGG | - | chr7.4:72315687-72315706 | MS.gene040430:intron | 35.0% | |
| TCTCTTCAACCTTCTTTCAA+AGG | - | chr7.4:72315407-72315426 | MS.gene040430:CDS | 35.0% | |
| TGAAACTTCTGATATGCTGA+AGG | - | chr7.4:72316096-72316115 | MS.gene040430:CDS | 35.0% | |
| TGAAAGAAGGTTGAAGAGAA+AGG | + | chr7.4:72315406-72315425 | None:intergenic | 35.0% | |
| ! | CTCTTCGGAAAAATGACTTT+TGG | + | chr7.4:72315014-72315033 | None:intergenic | 35.0% |
| ! | GACACAATTCCTTTGAAAGA+AGG | + | chr7.4:72315419-72315438 | None:intergenic | 35.0% |
| ! | TTGAGAAGTTGTTGTTGTGT+GGG | + | chr7.4:72315251-72315270 | None:intergenic | 35.0% |
| !! | CATTTTAGTAAGACCTCCAT+TGG | + | chr7.4:72316146-72316165 | None:intergenic | 35.0% |
| !! | TTCTGTCGATTTCAAGAACA+AGG | - | chr7.4:72315463-72315482 | MS.gene040430:CDS | 35.0% |
| AACTCGACTAGACTTTGTAG+CGG | - | chr7.4:72316288-72316307 | MS.gene040430:CDS | 40.0% | |
| AAGTGGAAGTAGGTGACTTT+AGG | - | chr7.4:72316044-72316063 | MS.gene040430:CDS | 40.0% | |
| AGAAGCTGTTGATGTTGAGT+GGG | + | chr7.4:72315062-72315081 | None:intergenic | 40.0% | |
| AGCACAACTTGTAGTGAAAG+TGG | + | chr7.4:72315200-72315219 | None:intergenic | 40.0% | |
| ATCTTTCGTCGATGGTGTAT+GGG | - | chr7.4:72315370-72315389 | MS.gene040430:CDS | 40.0% | |
| CAAGCTTCCTCTTGATAAAG+TGG | - | chr7.4:72316027-72316046 | MS.gene040430:CDS | 40.0% | |
| CCTACTTCCACTTTATCAAG+AGG | + | chr7.4:72316037-72316056 | None:intergenic | 40.0% | |
| CCTCTTGATAAAGTGGAAGT+AGG | - | chr7.4:72316034-72316053 | MS.gene040430:CDS | 40.0% | |
| CTTCACCTTGCTTATAAGGA+AGG | + | chr7.4:72315907-72315926 | None:intergenic | 40.0% | |
| GAAGAAGCAGAGAACAACTT+TGG | + | chr7.4:72314955-72314974 | None:intergenic | 40.0% | |
| GCTAATGCGCTGTACTTTAA+AGG | - | chr7.4:72315740-72315759 | MS.gene040430:intron | 40.0% | |
| GTCTCCGTCATTCTACAAAA+CGG | - | chr7.4:72315323-72315342 | MS.gene040430:CDS | 40.0% | |
| GTGTTCCAGTAACTCAGATT+CGG | + | chr7.4:72316008-72316027 | None:intergenic | 40.0% | |
| TATCTTTCGTCGATGGTGTA+TGG | - | chr7.4:72315369-72315388 | MS.gene040430:CDS | 40.0% | |
| TCCTTCCAAATGCACAAGAT+GGG | - | chr7.4:72315954-72315973 | MS.gene040430:CDS | 40.0% | |
| TTCCTTCCAAATGCACAAGA+TGG | - | chr7.4:72315953-72315972 | MS.gene040430:CDS | 40.0% | |
| ! | AGCACCGTTTTGTAGAATGA+CGG | + | chr7.4:72315330-72315349 | None:intergenic | 40.0% |
| ! | GTTGAGAAGTTGTTGTTGTG+TGG | + | chr7.4:72315252-72315271 | None:intergenic | 40.0% |
| ! | GTTTTACCTTTCACCAATGG+AGG | - | chr7.4:72316130-72316149 | MS.gene040430:CDS | 40.0% |
| ! | TGAGAAGTTGTTGTTGTGTG+GGG | + | chr7.4:72315250-72315269 | None:intergenic | 40.0% |
| !! | GTGGTTTTACCTTTCACCAA+TGG | - | chr7.4:72316127-72316146 | MS.gene040430:CDS | 40.0% |
| AAAGATGTTTGGCGATGGTG+AGG | + | chr7.4:72315136-72315155 | None:intergenic | 45.0% | |
| AAGATGTTTGGCGATGGTGA+GGG | + | chr7.4:72315135-72315154 | None:intergenic | 45.0% | |
| CCAGCTTCTCAAGCAAAGTT+GGG | + | chr7.4:72315982-72316001 | None:intergenic | 45.0% | |
| CCATCGACGAAAGATAGATG+AGG | + | chr7.4:72315365-72315384 | None:intergenic | 45.0% | |
| CCTCATCTATCTTTCGTCGA+TGG | - | chr7.4:72315362-72315381 | MS.gene040430:CDS | 45.0% | |
| GAGAAGCTGTTGATGTTGAG+TGG | + | chr7.4:72315063-72315082 | None:intergenic | 45.0% | |
| GATGTTGAGTGGGATCGATT+TGG | + | chr7.4:72315052-72315071 | None:intergenic | 45.0% | |
| GCATCTGATGCAGTCAGTAT+GGG | - | chr7.4:72316259-72316278 | MS.gene040430:CDS | 45.0% | |
| GCTACAAAGTCTAGTCGAGT+TGG | + | chr7.4:72316289-72316308 | None:intergenic | 45.0% | |
| GGAGAAAAGATGTTTGGCGA+TGG | + | chr7.4:72315141-72315160 | None:intergenic | 45.0% | |
| GTAAGACCTCCATTGGTGAA+AGG | + | chr7.4:72316139-72316158 | None:intergenic | 45.0% | |
| GTTGCTATGGTTGTTTGCGA+GGG | + | chr7.4:72315108-72315127 | None:intergenic | 45.0% | |
| TCTGATATGCTGAAGGAGCT+AGG | - | chr7.4:72316103-72316122 | MS.gene040430:CDS | 45.0% | |
| TGGGCTGAAAGAGAGACAAA+CGG | - | chr7.4:72315659-72315678 | MS.gene040430:CDS | 45.0% | |
| ! | CATACTGACTGCATCAGATG+CGG | + | chr7.4:72316260-72316279 | None:intergenic | 45.0% |
| ! | CGCTGTACTTTAAAGGAGAG+TGG | - | chr7.4:72315747-72315766 | MS.gene040430:intron | 45.0% |
| ! | CTTGGCCTTCCTTATAAGCA+AGG | - | chr7.4:72315899-72315918 | MS.gene040430:CDS | 45.0% |
| ! | GTTGATCGGTGGATTTGGAT+TGG | + | chr7.4:72315280-72315299 | None:intergenic | 45.0% |
| ! | TTTGTAGAATGACGGAGACG+AGG | + | chr7.4:72315322-72315341 | None:intergenic | 45.0% |
| !! | AGAACTTCTTCCTCTAGGGT+CGG | - | chr7.4:72315691-72315710 | MS.gene040430:intron | 45.0% |
| !! | GTTGAGTTGATCGGTGGATT+TGG | + | chr7.4:72315285-72315304 | None:intergenic | 45.0% |
| AGGTGCTCAATCCTCTTGCT+GGG | - | chr7.4:72316371-72316390 | MS.gene040430:CDS | 50.0% | |
| CCCAACTTTGCTTGAGAAGC+TGG | - | chr7.4:72315979-72315998 | MS.gene040430:CDS | 50.0% | |
| CGCATCTGATGCAGTCAGTA+TGG | - | chr7.4:72316258-72316277 | MS.gene040430:CDS | 50.0% | |
| CGTTGCTATGGTTGTTTGCG+AGG | + | chr7.4:72315109-72315128 | None:intergenic | 50.0% | |
| CTTCTTCGTGCTCAACATCG+TGG | - | chr7.4:72314968-72314987 | MS.gene040430:CDS | 50.0% | |
| GCAGAGAACAACTTTGGCGA+TGG | + | chr7.4:72314949-72314968 | None:intergenic | 50.0% | |
| GCCAGCTTCTCAAGCAAAGT+TGG | + | chr7.4:72315983-72316002 | None:intergenic | 50.0% | |
| GCCCATCTTGTGCATTTGGA+AGG | + | chr7.4:72315958-72315977 | None:intergenic | 50.0% | |
| GGCTTCCGAATCTGAGTTAC+TGG | - | chr7.4:72316000-72316019 | MS.gene040430:CDS | 50.0% | |
| GGTCGGTCGACAACTTAACT+CGG | - | chr7.4:72315708-72315727 | MS.gene040430:intron | 50.0% | |
| GTGCTAAGCATCATCGCTTC+TGG | - | chr7.4:72315215-72315234 | MS.gene040430:CDS | 50.0% | |
| TGGTGAGGGAAACGTTGCTA+TGG | + | chr7.4:72315121-72315140 | None:intergenic | 50.0% | |
| TTGCGAGGGAGGATTCTGAT+CGG | + | chr7.4:72315094-72315113 | None:intergenic | 50.0% | |
| TTTCGTCGATGGTGTATGGG+TGG | - | chr7.4:72315373-72315392 | MS.gene040430:CDS | 50.0% | |
| ! | AATGACGGAGACGAGGATAG+AGG | + | chr7.4:72315315-72315334 | None:intergenic | 50.0% |
| ! | GATCGGTGGATTTGGATTGG+AGG | + | chr7.4:72315277-72315296 | None:intergenic | 50.0% |
| !! | GAAGGAGTTGAGTTGATCGG+TGG | + | chr7.4:72315291-72315310 | None:intergenic | 50.0% |
| !! | GGCGAAGGAGTTGAGTTGAT+CGG | + | chr7.4:72315294-72315313 | None:intergenic | 50.0% |
| AAGTTGTCGACCGACCCTAG+AGG | + | chr7.4:72315704-72315723 | None:intergenic | 55.0% | |
| ACTGACTGCATCAGATGCGG+TGG | + | chr7.4:72316257-72316276 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | - | chr7.4:72316370-72316389 | MS.gene040430:CDS | 55.0% | |
| GCTATGGTTGTTTGCGAGGG+AGG | + | chr7.4:72315105-72315124 | None:intergenic | 55.0% | |
| GGGAGCCCATCTTGTGCATT+TGG | + | chr7.4:72315962-72315981 | None:intergenic | 55.0% | |
| TATGCTGAAGGAGCTAGGCG+TGG | - | chr7.4:72316108-72316127 | MS.gene040430:CDS | 55.0% | |
| ! | TTGTTGTTGTGTGGGGCCCT+CGG | + | chr7.4:72315243-72315262 | None:intergenic | 55.0% |
| !! | ATCATCGCTTCTGGCTCCGA+GGG | - | chr7.4:72315224-72315243 | MS.gene040430:CDS | 55.0% |
| AGATAGATGAGGCCCGCCTG+CGG | + | chr7.4:72315354-72315373 | None:intergenic | 60.0% | |
| CGAGGGAGGATTCTGATCGG+AGG | + | chr7.4:72315091-72315110 | None:intergenic | 60.0% | |
| CTACAAAACGGTGCTCCCGC+AGG | - | chr7.4:72315335-72315354 | MS.gene040430:CDS | 60.0% | |
| GGAGACGAGGATAGAGGCGA+AGG | + | chr7.4:72315309-72315328 | None:intergenic | 60.0% | |
| TGCGGTGGCTGCAACAGCTT+CGG | + | chr7.4:72316242-72316261 | None:intergenic | 60.0% | |
| !! | CATCATCGCTTCTGGCTCCG+AGG | - | chr7.4:72315223-72315242 | MS.gene040430:CDS | 60.0% |
| AAAACGGTGCTCCCGCAGGC+GGG | - | chr7.4:72315339-72315358 | MS.gene040430:CDS | 65.0% | |
| CAAAACGGTGCTCCCGCAGG+CGG | - | chr7.4:72315338-72315357 | MS.gene040430:CDS | 65.0% | |
| GATAGATGAGGCCCGCCTGC+GGG | + | chr7.4:72315353-72315372 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72314921 | 72316396 | 72314921 | ID=MS.gene040430 |
| chr7.4 | mRNA | 72314921 | 72316396 | 72314921 | ID=MS.gene040430.t1;Parent=MS.gene040430 |
| chr7.4 | exon | 72315833 | 72316396 | 72315833 | ID=MS.gene040430.t1.exon1;Parent=MS.gene040430.t1 |
| chr7.4 | CDS | 72315833 | 72316396 | 72315833 | ID=cds.MS.gene040430.t1;Parent=MS.gene040430.t1 |
| chr7.4 | exon | 72314921 | 72315688 | 72314921 | ID=MS.gene040430.t1.exon2;Parent=MS.gene040430.t1 |
| chr7.4 | CDS | 72314921 | 72315688 | 72314921 | ID=cds.MS.gene040430.t1;Parent=MS.gene040430.t1 |
| Gene Sequence |
| Protein sequence |
