Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041625.t1 | XP_003609722.2 | 99 | 1109 | 10 | 1 | 1 | 1109 | 1 | 1108 | 0.00E+00 | 2213.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041625.t1 | Q9LRZ0 | 75.7 | 1109 | 258 | 7 | 1 | 1103 | 1 | 1103 | 0.0e+00 | 1704.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041625.t1 | G7JMQ4 | 99.0 | 1109 | 10 | 1 | 1 | 1109 | 1 | 1108 | 0.0e+00 | 2213.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041625 | MS.gene026732 | PPI |
| MS.gene041625 | MS.gene86946 | PPI |
| MS.gene041625 | MS.gene61719 | PPI |
| MS.gene060813 | MS.gene041625 | PPI |
| MS.gene86431 | MS.gene041625 | PPI |
| MS.gene041625 | MS.gene83555 | PPI |
| MS.gene041625 | MS.gene034106 | PPI |
| MS.gene041625 | MS.gene60151 | PPI |
| MS.gene067429 | MS.gene041625 | PPI |
| MS.gene041625 | MS.gene036741 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041625.t1 | MTR_4g120900 | 99.008 | 1109 | 10 | 1 | 1 | 1109 | 1 | 1108 | 0.0 | 2288 |
| MS.gene041625.t1 | MTR_1g083700 | 73.405 | 1113 | 280 | 9 | 1 | 1105 | 1 | 1105 | 0.0 | 1704 |
| MS.gene041625.t1 | MTR_7g112460 | 74.104 | 1116 | 269 | 9 | 1 | 1105 | 1 | 1107 | 0.0 | 1693 |
| MS.gene041625.t1 | MTR_4g009840 | 62.401 | 1133 | 388 | 18 | 1 | 1109 | 1 | 1119 | 0.0 | 1412 |
| MS.gene041625.t1 | MTR_2g104140 | 62.246 | 1131 | 392 | 17 | 1 | 1109 | 16 | 1133 | 0.0 | 1405 |
| MS.gene041625.t1 | MTR_2g104140 | 62.246 | 1131 | 391 | 17 | 1 | 1109 | 16 | 1132 | 0.0 | 1405 |
| MS.gene041625.t1 | MTR_2g104140 | 62.246 | 1131 | 391 | 17 | 1 | 1109 | 1 | 1117 | 0.0 | 1403 |
| MS.gene041625.t1 | MTR_2g104140 | 62.143 | 1120 | 388 | 16 | 1 | 1109 | 16 | 1110 | 0.0 | 1392 |
| MS.gene041625.t1 | MTR_4g114980 | 60.777 | 1132 | 401 | 17 | 1 | 1105 | 1 | 1116 | 0.0 | 1391 |
| MS.gene041625.t1 | MTR_2g104140 | 62.143 | 1120 | 388 | 16 | 1 | 1109 | 1 | 1095 | 0.0 | 1390 |
| MS.gene041625.t1 | MTR_4g114980 | 60.714 | 1120 | 401 | 17 | 1 | 1105 | 1 | 1096 | 0.0 | 1369 |
| MS.gene041625.t1 | MTR_2g435370 | 53.581 | 1103 | 452 | 13 | 1 | 1097 | 1 | 1049 | 0.0 | 1181 |
| MS.gene041625.t1 | MTR_2g435440 | 48.607 | 1113 | 410 | 14 | 1 | 1098 | 1 | 966 | 0.0 | 982 |
| MS.gene041625.t1 | MTR_2g065670 | 54.386 | 399 | 147 | 7 | 238 | 630 | 335 | 704 | 2.78e-128 | 409 |
| MS.gene041625.t1 | MTR_2g065670 | 49.004 | 251 | 97 | 5 | 28 | 275 | 1 | 223 | 2.87e-65 | 236 |
| MS.gene041625.t1 | MTR_1g012820 | 43.342 | 383 | 203 | 10 | 734 | 1109 | 245 | 620 | 1.54e-91 | 306 |
| MS.gene041625.t1 | MTR_1g012820 | 54.592 | 196 | 86 | 1 | 8 | 203 | 9 | 201 | 2.34e-62 | 224 |
| MS.gene041625.t1 | MTR_2g435380 | 47.653 | 277 | 107 | 6 | 290 | 553 | 115 | 366 | 1.56e-73 | 249 |
| MS.gene041625.t1 | MTR_1g019450 | 50.867 | 173 | 85 | 0 | 8 | 180 | 10 | 182 | 7.35e-55 | 189 |
| MS.gene041625.t1 | MTR_1g019470 | 48.555 | 173 | 89 | 0 | 7 | 179 | 9 | 181 | 3.38e-53 | 185 |
| MS.gene041625.t1 | MTR_4g109730 | 62.136 | 103 | 39 | 0 | 8 | 110 | 9 | 111 | 6.52e-36 | 137 |
| MS.gene041625.t1 | MTR_7g069750 | 80.328 | 61 | 12 | 0 | 100 | 160 | 11 | 71 | 2.06e-25 | 100 |
| MS.gene041625.t1 | MTR_7g056777 | 41.322 | 121 | 65 | 2 | 25 | 139 | 20 | 140 | 3.97e-23 | 96.7 |
| MS.gene041625.t1 | MTR_7g056757 | 51.190 | 84 | 37 | 3 | 1 | 83 | 1 | 81 | 2.83e-19 | 83.6 |
| MS.gene041625.t1 | MTR_7g007420 | 57.353 | 68 | 27 | 2 | 178 | 243 | 98 | 165 | 8.70e-17 | 80.1 |
| MS.gene041625.t1 | MTR_6g033840 | 62.712 | 59 | 16 | 1 | 529 | 587 | 1 | 53 | 4.08e-15 | 71.6 |
| MS.gene041625.t1 | MTR_7g056753 | 64.286 | 42 | 15 | 0 | 27 | 68 | 43 | 84 | 3.53e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041625.t1 | AT3G16830 | 75.271 | 1108 | 264 | 6 | 1 | 1103 | 1 | 1103 | 0.0 | 1750 |
| MS.gene041625.t1 | AT5G27030 | 69.203 | 1104 | 316 | 10 | 1 | 1099 | 1 | 1085 | 0.0 | 1562 |
| MS.gene041625.t1 | AT5G27030 | 67.611 | 1130 | 316 | 11 | 1 | 1099 | 1 | 1111 | 0.0 | 1546 |
| MS.gene041625.t1 | AT1G80490 | 61.082 | 1128 | 394 | 17 | 1 | 1109 | 1 | 1102 | 0.0 | 1391 |
| MS.gene041625.t1 | AT1G80490 | 61.082 | 1128 | 394 | 17 | 1 | 1109 | 1 | 1102 | 0.0 | 1391 |
| MS.gene041625.t1 | AT1G15750 | 61.593 | 1130 | 396 | 19 | 1 | 1109 | 1 | 1113 | 0.0 | 1389 |
| MS.gene041625.t1 | AT1G15750 | 61.593 | 1130 | 396 | 19 | 1 | 1109 | 1 | 1113 | 0.0 | 1389 |
| MS.gene041625.t1 | AT1G15750 | 61.593 | 1130 | 396 | 19 | 1 | 1109 | 1 | 1113 | 0.0 | 1389 |
| MS.gene041625.t1 | AT1G15750 | 61.593 | 1130 | 396 | 19 | 1 | 1109 | 1 | 1113 | 0.0 | 1389 |
| MS.gene041625.t1 | AT1G80490 | 60.958 | 1127 | 396 | 16 | 1 | 1109 | 1 | 1101 | 0.0 | 1389 |
| MS.gene041625.t1 | AT3G15880 | 60.301 | 1131 | 410 | 18 | 1 | 1105 | 1 | 1118 | 0.0 | 1370 |
| MS.gene041625.t1 | AT3G15880 | 60.301 | 1131 | 410 | 18 | 1 | 1105 | 1 | 1118 | 0.0 | 1370 |
| MS.gene041625.t1 | AT3G15880 | 60.301 | 1131 | 410 | 18 | 1 | 1105 | 1 | 1118 | 0.0 | 1370 |
| MS.gene041625.t1 | AT3G15880 | 59.416 | 1131 | 410 | 19 | 1 | 1105 | 1 | 1108 | 0.0 | 1339 |
| MS.gene041625.t1 | AT2G25420 | 36.264 | 364 | 214 | 9 | 746 | 1101 | 371 | 724 | 1.22e-65 | 237 |
| MS.gene041625.t1 | AT2G25420 | 38.509 | 161 | 98 | 1 | 6 | 166 | 9 | 168 | 5.50e-26 | 115 |
| MS.gene041625.t1 | AT2G25420 | 31.461 | 178 | 84 | 2 | 4 | 181 | 191 | 330 | 2.82e-18 | 90.9 |
| MS.gene041625.t1 | AT2G25420 | 35.849 | 371 | 213 | 10 | 746 | 1101 | 371 | 731 | 5.96e-65 | 235 |
| MS.gene041625.t1 | AT2G25420 | 38.509 | 161 | 98 | 1 | 6 | 166 | 9 | 168 | 5.75e-26 | 115 |
| MS.gene041625.t1 | AT2G25420 | 25.503 | 298 | 159 | 7 | 4 | 280 | 191 | 446 | 1.95e-18 | 91.7 |
| MS.gene041625.t1 | AT2G25420 | 34.756 | 328 | 196 | 8 | 746 | 1066 | 371 | 687 | 3.34e-53 | 199 |
| MS.gene041625.t1 | AT2G25420 | 38.509 | 161 | 98 | 1 | 6 | 166 | 9 | 168 | 1.69e-25 | 114 |
| MS.gene041625.t1 | AT2G25420 | 31.461 | 178 | 84 | 2 | 4 | 181 | 191 | 330 | 5.66e-18 | 90.1 |
| MS.gene041625.t1 | AT2G25420 | 34.328 | 335 | 195 | 9 | 746 | 1066 | 371 | 694 | 1.74e-52 | 197 |
| MS.gene041625.t1 | AT2G25420 | 38.509 | 161 | 98 | 1 | 6 | 166 | 9 | 168 | 1.61e-25 | 114 |
| MS.gene041625.t1 | AT2G25420 | 25.503 | 298 | 159 | 7 | 4 | 280 | 191 | 446 | 2.47e-18 | 91.3 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32533 | gene | 9256 | 17052 | 9256 | ID=MS.gene041625 |
| 32533 | mRNA | 9256 | 17052 | 9256 | ID=MS.gene041625.t1;Parent=MS.gene041625 |
| 32533 | exon | 9256 | 9335 | 9256 | ID=MS.gene041625.t1.exon1;Parent=MS.gene041625.t1 |
| 32533 | CDS | 9256 | 9335 | 9256 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 9440 | 9619 | 9440 | ID=MS.gene041625.t1.exon2;Parent=MS.gene041625.t1 |
| 32533 | CDS | 9440 | 9619 | 9440 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 10137 | 10250 | 10137 | ID=MS.gene041625.t1.exon3;Parent=MS.gene041625.t1 |
| 32533 | CDS | 10137 | 10250 | 10137 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 10328 | 10486 | 10328 | ID=MS.gene041625.t1.exon4;Parent=MS.gene041625.t1 |
| 32533 | CDS | 10328 | 10486 | 10328 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 10587 | 10758 | 10587 | ID=MS.gene041625.t1.exon5;Parent=MS.gene041625.t1 |
| 32533 | CDS | 10587 | 10758 | 10587 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 10863 | 10992 | 10863 | ID=MS.gene041625.t1.exon6;Parent=MS.gene041625.t1 |
| 32533 | CDS | 10863 | 10992 | 10863 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 11432 | 11541 | 11432 | ID=MS.gene041625.t1.exon7;Parent=MS.gene041625.t1 |
| 32533 | CDS | 11432 | 11541 | 11432 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 11638 | 11779 | 11638 | ID=MS.gene041625.t1.exon8;Parent=MS.gene041625.t1 |
| 32533 | CDS | 11638 | 11779 | 11638 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 11866 | 11975 | 11866 | ID=MS.gene041625.t1.exon9;Parent=MS.gene041625.t1 |
| 32533 | CDS | 11866 | 11975 | 11866 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 12334 | 12403 | 12334 | ID=MS.gene041625.t1.exon10;Parent=MS.gene041625.t1 |
| 32533 | CDS | 12334 | 12403 | 12334 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 12480 | 12553 | 12480 | ID=MS.gene041625.t1.exon11;Parent=MS.gene041625.t1 |
| 32533 | CDS | 12480 | 12553 | 12480 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 12655 | 12750 | 12655 | ID=MS.gene041625.t1.exon12;Parent=MS.gene041625.t1 |
| 32533 | CDS | 12655 | 12750 | 12655 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 12836 | 12928 | 12836 | ID=MS.gene041625.t1.exon13;Parent=MS.gene041625.t1 |
| 32533 | CDS | 12836 | 12928 | 12836 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 13357 | 13484 | 13357 | ID=MS.gene041625.t1.exon14;Parent=MS.gene041625.t1 |
| 32533 | CDS | 13357 | 13484 | 13357 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 13607 | 13841 | 13607 | ID=MS.gene041625.t1.exon15;Parent=MS.gene041625.t1 |
| 32533 | CDS | 13607 | 13841 | 13607 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 13919 | 14077 | 13919 | ID=MS.gene041625.t1.exon16;Parent=MS.gene041625.t1 |
| 32533 | CDS | 13919 | 14077 | 13919 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 14163 | 14258 | 14163 | ID=MS.gene041625.t1.exon17;Parent=MS.gene041625.t1 |
| 32533 | CDS | 14163 | 14258 | 14163 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 14446 | 14598 | 14446 | ID=MS.gene041625.t1.exon18;Parent=MS.gene041625.t1 |
| 32533 | CDS | 14446 | 14598 | 14446 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 14699 | 14809 | 14699 | ID=MS.gene041625.t1.exon19;Parent=MS.gene041625.t1 |
| 32533 | CDS | 14699 | 14809 | 14699 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 15285 | 15461 | 15285 | ID=MS.gene041625.t1.exon20;Parent=MS.gene041625.t1 |
| 32533 | CDS | 15285 | 15461 | 15285 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 15561 | 15689 | 15561 | ID=MS.gene041625.t1.exon21;Parent=MS.gene041625.t1 |
| 32533 | CDS | 15561 | 15689 | 15561 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 15766 | 15861 | 15766 | ID=MS.gene041625.t1.exon22;Parent=MS.gene041625.t1 |
| 32533 | CDS | 15766 | 15861 | 15766 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 16010 | 16192 | 16010 | ID=MS.gene041625.t1.exon23;Parent=MS.gene041625.t1 |
| 32533 | CDS | 16010 | 16192 | 16010 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 16405 | 16580 | 16405 | ID=MS.gene041625.t1.exon24;Parent=MS.gene041625.t1 |
| 32533 | CDS | 16405 | 16580 | 16405 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| 32533 | exon | 16897 | 17052 | 16897 | ID=MS.gene041625.t1.exon25;Parent=MS.gene041625.t1 |
| 32533 | CDS | 16897 | 17052 | 16897 | ID=cds.MS.gene041625.t1;Parent=MS.gene041625.t1 |
| Gene Sequence |
| Protein sequence |
