AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041681

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041681.t1 MTR_6g075250 96.000 250 10 0 1 250 1 250 4.65e-159 441
MS.gene041681.t1 MTR_5g058150 72.908 251 65 3 1 249 1 250 4.33e-116 332
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041681.t1 AT1G12650 51.613 248 114 4 1 245 1 245 6.54e-61 192
MS.gene041681.t1 AT1G12650 51.613 248 114 4 1 245 1 245 6.54e-61 192
MS.gene041681.t1 AT1G12650 51.613 248 114 4 1 245 1 245 6.54e-61 192
MS.gene041681.t1 AT1G12650 51.613 248 114 4 1 245 1 245 6.54e-61 192

Find 44 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATTGAGGCAGAGATTT+TGG 0.212404 6.3:+69844590 MS.gene041681:CDS
TTACATTTCTTGAGATAAAA+AGG 0.234510 6.3:-69844660 None:intergenic
TCAATATTTCTCGCTGAATC+AGG 0.323233 6.3:-69844576 None:intergenic
CCACAATGGGATTCAAATCT+TGG 0.346873 6.3:-69843137 None:intergenic
TAGCATATGGCATAAATCTA+TGG 0.351847 6.3:-69845854 None:intergenic
GAGAAGACAAGAAGTTAAAA+AGG 0.353474 6.3:+69842388 MS.gene041681:CDS
AGAATCAGGATCACCTTCTT+AGG 0.356324 6.3:-69842693 None:intergenic
TCTATTTCACTTCTGCGCTC+TGG 0.390410 6.3:-69844426 None:intergenic
TCTTCACCATCTCAGGCATC+TGG 0.421934 6.3:+69845780 MS.gene041681:intron
CCTTACTATTCGTTGTCAAC+AGG 0.423569 6.3:-69845879 None:intergenic
TTGCAGAACGCTCGATCAAA+CGG 0.431952 6.3:+69842339 MS.gene041681:CDS
GTGATCTACTTGACTCTAAT+CGG 0.442877 6.3:+69843159 MS.gene041681:CDS
GGATAAAACAGACTCTTAAG+AGG 0.443274 6.3:+69844382 MS.gene041681:intron
TTTCAATAAGCCTTTGCCTT+CGG 0.444585 6.3:-69845655 None:intergenic
AAGCTCGGAAGTGGAATTCC+AGG 0.460429 6.3:+69842084 MS.gene041681:CDS
TTGATTCATATATTGAGAAA+AGG 0.461564 6.3:+69845808 MS.gene041681:CDS
CAGTATCACTCTCCTTGTAC+TGG 0.468021 6.3:-69842119 None:intergenic
TACTGGATTTCACTGGAACC+TGG 0.471299 6.3:-69842102 None:intergenic
AATACTACCTTTCTTTCAGC+AGG 0.473261 6.3:-69844291 None:intergenic
TTGTCAACAGGTCTAGCATA+TGG 0.475949 6.3:-69845867 None:intergenic
GAAACAAAGAGAAGCAGCAA+AGG 0.480456 6.3:+69844626 MS.gene041681:CDS
GGAGGAGGAAGAGATAATGA+AGG 0.487055 6.3:+69842290 MS.gene041681:intron
CTCCTTGTACTGGATTTCAC+TGG 0.490528 6.3:-69842109 None:intergenic
TGATCTTCCTGCTGAAAGAA+AGG 0.494790 6.3:+69844284 MS.gene041681:CDS
ACAAAGAGAAGCAGCAAAGG+AGG 0.495958 6.3:+69844629 MS.gene041681:CDS
TTCTTGCTACTAGCCTCCAT+TGG 0.499150 6.3:-69842636 None:intergenic
AAATAGAAGAACTCGTATCA+TGG 0.503092 6.3:+69844442 MS.gene041681:CDS
GTCAAGTAGATCACCACAAT+GGG 0.514593 6.3:-69843150 None:intergenic
AAAATGAAGAAGCTCGGAAG+TGG 0.520702 6.3:+69842075 None:intergenic
TTTCTCAAAATGAAGAAGCT+CGG 0.540948 6.3:+69842069 None:intergenic
TGTCATCCATGCTCCTAAGA+AGG 0.547786 6.3:+69842680 MS.gene041681:CDS
CTAATGTTTGAATAGGCCAA+TGG 0.565708 6.3:+69842620 MS.gene041681:intron
CAGCTGAAATCCGAAGGCAA+AGG 0.566007 6.3:+69845645 MS.gene041681:intron
CAAAGAGAAGCAGCAAAGGA+GGG 0.567185 6.3:+69844630 MS.gene041681:CDS
GAGGAAGAGAAATGCTGCAA+AGG 0.582512 6.3:+69845830 MS.gene041681:CDS
ATTCATATATTGAGAAAAGG+AGG 0.585349 6.3:+69845811 MS.gene041681:CDS
GGATCACCTTCTTAGGAGCA+TGG 0.586270 6.3:-69842686 None:intergenic
GGAGGCTAGTAGCAAGAAGC+CGG 0.591480 6.3:+69842641 MS.gene041681:CDS
GATAAAACAGACTCTTAAGA+GGG 0.593205 6.3:+69844383 MS.gene041681:intron
TTCCAGTGAAATCCAGTACA+AGG 0.601473 6.3:+69842107 MS.gene041681:CDS
TGATTCAGCGAGAAATATTG+AGG 0.611514 6.3:+69844578 MS.gene041681:CDS
AGTCAAGTAGATCACCACAA+TGG 0.634966 6.3:-69843151 None:intergenic
ATGTTTGAATAGGCCAATGG+AGG 0.654355 6.3:+69842623 MS.gene041681:intron
CCAAGATTTGAATCCCATTG+TGG 0.675566 6.3:+69843137 MS.gene041681:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATAATTTAAAATACTTG+AGG - chr6.3:69843441-69843460 None:intergenic 10.0%
!!! TATTTTTTAAGATAATGTTT+CGG - chr6.3:69845009-69845028 None:intergenic 10.0%
!! AAAAAAATGTCATTATATGT+AGG - chr6.3:69845616-69845635 None:intergenic 15.0%
!! AAAATTAGTATTCAATTGTA+CGG + chr6.3:69844091-69844110 MS.gene041681:intron 15.0%
!! ATAATTTCTAATGTTTGAAT+AGG + chr6.3:69842613-69842632 MS.gene041681:intron 15.0%
!!! AGATGAAAAATAAGTTTTAA+AGG + chr6.3:69844835-69844854 MS.gene041681:intron 15.0%
!!! TTTTAAATTATATAAGACCT+AGG + chr6.3:69843447-69843466 MS.gene041681:intron 15.0%
!! AAAACGGAAATAAAATGATA+AGG - chr6.3:69844700-69844719 None:intergenic 20.0%
!! AAATTGTCTAAATCATAGTA+TGG - chr6.3:69843669-69843688 None:intergenic 20.0%
!! ATATAGAAAGTGAAAGAAAA+GGG - chr6.3:69844776-69844795 None:intergenic 20.0%
!! ATCGAATTCAAAAATCTAAA+CGG - chr6.3:69845494-69845513 None:intergenic 20.0%
!! TAAAAAGGCTTAATAAGAAT+AGG + chr6.3:69842403-69842422 MS.gene041681:CDS 20.0%
!! TGAAAAATATAAACATCTCA+AGG + chr6.3:69845673-69845692 MS.gene041681:CDS 20.0%
!! TGAAGAATAGAAAGAATTAA+TGG - chr6.3:69845766-69845785 None:intergenic 20.0%
!! TTACATTTCTTGAGATAAAA+AGG - chr6.3:69844663-69844682 None:intergenic 20.0%
!! TTCAAATCAAAATAGTTTCA+TGG + chr6.3:69842875-69842894 MS.gene041681:intron 20.0%
!! TTGATTCATATATTGAGAAA+AGG + chr6.3:69845808-69845827 MS.gene041681:CDS 20.0%
!!! CCATATTTTTTTTAGCTTTT+CGG + chr6.3:69845178-69845197 MS.gene041681:intron 20.0%
!!! GATATTCTGTTTTATATATG+TGG + chr6.3:69843061-69843080 MS.gene041681:intron 20.0%
!!! TAAATCATTTGATGCTTTTT+TGG - chr6.3:69843634-69843653 None:intergenic 20.0%
!!! TGAAAAATAAGTTTTAAAGG+TGG + chr6.3:69844838-69844857 MS.gene041681:intron 20.0%
!!! TGATTTTGTCAAATTATTAG+CGG - chr6.3:69843886-69843905 None:intergenic 20.0%
!!! TTACATCATTTTTAGCTTTT+TGG + chr6.3:69845150-69845169 MS.gene041681:intron 20.0%
!!! TTAGGTGTTTTTTTTTATCT+GGG + chr6.3:69845426-69845445 MS.gene041681:intron 20.0%
!!! TTATTGATTTTGATGATTGT+AGG + chr6.3:69842269-69842288 MS.gene041681:intron 20.0%
!!! TTTAGGTGTTTTTTTTTATC+TGG + chr6.3:69845425-69845444 MS.gene041681:intron 20.0%
! ACTTATTGAGAATGTGTTAA+AGG + chr6.3:69843490-69843509 MS.gene041681:intron 25.0%
! ATTCATATATTGAGAAAAGG+AGG + chr6.3:69845811-69845830 MS.gene041681:CDS 25.0%
! ATTCTAACACAACAAGAAAA+AGG - chr6.3:69843217-69843236 None:intergenic 25.0%
! CATATAGAAAGTGAAAGAAA+AGG - chr6.3:69844777-69844796 None:intergenic 25.0%
! CCGAAAAGCTAAAAAAAATA+TGG - chr6.3:69845181-69845200 None:intergenic 25.0%
! GAATTCGATATACAATTCTT+TGG + chr6.3:69845505-69845524 MS.gene041681:intron 25.0%
! GATAATATAGTATTCTTTGC+TGG + chr6.3:69843243-69843262 MS.gene041681:intron 25.0%
! GCACTAATAATATAAGTACT+TGG + chr6.3:69844220-69844239 MS.gene041681:intron 25.0%
! TAAAGAATGTGTAAAATGTG+TGG - chr6.3:69845046-69845065 None:intergenic 25.0%
! TAAGTGTAATTATATGTGTC+AGG + chr6.3:69843912-69843931 MS.gene041681:intron 25.0%
! TCGAATTCAAAAATCTAAAC+GGG - chr6.3:69845493-69845512 None:intergenic 25.0%
! TTGGCAATTCATTGAATTTA+TGG + chr6.3:69845524-69845543 MS.gene041681:intron 25.0%
!! ATATCTAAAGTGAGAGAAAA+CGG - chr6.3:69844716-69844735 None:intergenic 25.0%
!! CAACTTTTACGATATTATGT+TGG + chr6.3:69843730-69843749 MS.gene041681:intron 25.0%
!! TTGTTGCTTCTACAAAATTA+TGG + chr6.3:69842799-69842818 MS.gene041681:intron 25.0%
!!! AAGCCATTAGCTTTTATTTT+TGG + chr6.3:69842898-69842917 MS.gene041681:intron 25.0%
!!! AGCCATTAGCTTTTATTTTT+GGG + chr6.3:69842899-69842918 MS.gene041681:intron 25.0%
!!! ATTAGGTTTTGTCAAAATCA+TGG - chr6.3:69842777-69842796 None:intergenic 25.0%
!!! TTGAATACTAATTTTCAAGC+AGG - chr6.3:69844086-69844105 None:intergenic 25.0%
AAAATACTTGAGGAAACGTT+GGG - chr6.3:69843431-69843450 None:intergenic 30.0%
AAAGCAATGATGATCATTGA+GGG + chr6.3:69845106-69845125 MS.gene041681:intron 30.0%
AAATAGAAGAACTCGTATCA+TGG + chr6.3:69844442-69844461 MS.gene041681:CDS 30.0%
AAATCAATGTGTCAAGAATC+AGG - chr6.3:69842710-69842729 None:intergenic 30.0%
AACTCAAACCAAGAACAAAA+AGG - chr6.3:69844022-69844041 None:intergenic 30.0%
ACTAATAAATGTCACACTTC+TGG - chr6.3:69844511-69844530 None:intergenic 30.0%
AGAACTTGTAGGTTTACTAT+AGG + chr6.3:69844808-69844827 MS.gene041681:intron 30.0%
AGTAATAGTTAGTGCATGTT+TGG + chr6.3:69842443-69842462 MS.gene041681:intron 30.0%
ATATAAGTACTTGGACTTAC+AGG + chr6.3:69844229-69844248 MS.gene041681:intron 30.0%
CAAAAACTCTTCATTAAGAG+AGG - chr6.3:69845345-69845364 None:intergenic 30.0%
CAATCAATTGAGTACATGTT+TGG - chr6.3:69842531-69842550 None:intergenic 30.0%
CAATTGATTGTTTGACAAAG+TGG + chr6.3:69842541-69842560 MS.gene041681:intron 30.0%
CGAATTCAAAAATCTAAACG+GGG - chr6.3:69845492-69845511 None:intergenic 30.0%
CGGAAATAAAATGATAAGGA+AGG - chr6.3:69844696-69844715 None:intergenic 30.0%
GAAACTATTCAGAGTATGTT+TGG - chr6.3:69842858-69842877 None:intergenic 30.0%
GAGAAGACAAGAAGTTAAAA+AGG + chr6.3:69842388-69842407 MS.gene041681:CDS 30.0%
GATAAAACAGACTCTTAAGA+GGG + chr6.3:69844383-69844402 MS.gene041681:intron 30.0%
GCAATAAATGACAGAATACA+AGG - chr6.3:69845734-69845753 None:intergenic 30.0%
GTCTAAATCATAGTATGGAT+TGG - chr6.3:69843664-69843683 None:intergenic 30.0%
GTTACAAACTTACTATGACA+TGG - chr6.3:69842232-69842251 None:intergenic 30.0%
TAAAATACTTGAGGAAACGT+TGG - chr6.3:69843432-69843451 None:intergenic 30.0%
TACAACCTGAAAATTGTTGA+AGG - chr6.3:69843111-69843130 None:intergenic 30.0%
TAGAGTAAACAATGAGCAAT+TGG - chr6.3:69842955-69842974 None:intergenic 30.0%
TAGCATATGGCATAAATCTA+TGG - chr6.3:69845857-69845876 None:intergenic 30.0%
TATTTGGTTATCATGAAGCA+CGG + chr6.3:69843820-69843839 MS.gene041681:intron 30.0%
TCAATAAGTAACATACTTGC+AGG - chr6.3:69843783-69843802 None:intergenic 30.0%
TGGCATTTGGTATCTTTATT+TGG + chr6.3:69843804-69843823 MS.gene041681:intron 30.0%
TTACGATATTATGTTGGTGT+TGG + chr6.3:69843736-69843755 MS.gene041681:intron 30.0%
TTGTAGAAGCAACAAAGATT+AGG - chr6.3:69842794-69842813 None:intergenic 30.0%
! AAATATTGAGGCAGAGATTT+TGG + chr6.3:69844590-69844609 MS.gene041681:CDS 30.0%
! ACAATGAGCAATTGGTTTAT+AGG - chr6.3:69842947-69842966 None:intergenic 30.0%
! ATATGGTTCCTTTTTGTTCT+TGG + chr6.3:69844011-69844030 MS.gene041681:intron 30.0%
! CAAGTATGTTACTTATTGAG+TGG + chr6.3:69843784-69843803 MS.gene041681:intron 30.0%
! CAATGAGCAATTGGTTTATA+GGG - chr6.3:69842946-69842965 None:intergenic 30.0%
! CTCTCTTAATGAAGAGTTTT+TGG + chr6.3:69845343-69845362 MS.gene041681:intron 30.0%
! TCACATTTACATCTCTTTTC+AGG + chr6.3:69844539-69844558 MS.gene041681:intron 30.0%
! TTTCGTCTAATGACATTTTC+TGG + chr6.3:69844361-69844380 MS.gene041681:intron 30.0%
!! TTGATTTTGATGATTGTAGG+AGG + chr6.3:69842272-69842291 MS.gene041681:intron 30.0%
!!! CATTGATTTCATGTTTTGGT+TGG + chr6.3:69842721-69842740 MS.gene041681:intron 30.0%
!!! CCATTAGCTTTTATTTTTGG+GGG + chr6.3:69842901-69842920 MS.gene041681:intron 30.0%
!!! GACACATTGATTTCATGTTT+TGG + chr6.3:69842717-69842736 MS.gene041681:intron 30.0%
!!! GCCATTAGCTTTTATTTTTG+GGG + chr6.3:69842900-69842919 MS.gene041681:intron 30.0%
AATACTACCTTTCTTTCAGC+AGG - chr6.3:69844294-69844313 None:intergenic 35.0%
AATGACAGAATACAAGGTGA+AGG - chr6.3:69845728-69845747 None:intergenic 35.0%
ACTTGTAGGTTTACTATAGG+AGG + chr6.3:69844811-69844830 MS.gene041681:intron 35.0%
AGTGGTAAAGGATAAGCTTT+AGG + chr6.3:69845377-69845396 MS.gene041681:intron 35.0%
ATGACAGAATACAAGGTGAA+GGG - chr6.3:69845727-69845746 None:intergenic 35.0%
CACTTTCTATATGCTAGAGT+AGG + chr6.3:69844784-69844803 MS.gene041681:intron 35.0%
CCCCCAAAAATAAAAGCTAA+TGG - chr6.3:69842904-69842923 None:intergenic 35.0%
CGGTGAGTTTGATAAAATCA+CGG - chr6.3:69842830-69842849 None:intergenic 35.0%
CTAATGTTTGAATAGGCCAA+TGG + chr6.3:69842620-69842639 MS.gene041681:intron 35.0%
CTGCTCAGTTAGATTAAGTT+GGG + chr6.3:69844875-69844894 MS.gene041681:intron 35.0%
GAAAGCAATGATGATCATTG+AGG + chr6.3:69845105-69845124 MS.gene041681:intron 35.0%
GAGTAACTTTGCCAAAATCA+AGG - chr6.3:69842503-69842522 None:intergenic 35.0%
GGATAAAACAGACTCTTAAG+AGG + chr6.3:69844382-69844401 MS.gene041681:intron 35.0%
GTGATCTACTTGACTCTAAT+CGG + chr6.3:69843159-69843178 MS.gene041681:CDS 35.0%
GTGTTAAAGGCTTAAATCCA+TGG + chr6.3:69843503-69843522 MS.gene041681:intron 35.0%
GTTACTTATTGAGTGGCATT+TGG + chr6.3:69843791-69843810 MS.gene041681:intron 35.0%
TAATGTCGTGTCCTTTAAGT+AGG + chr6.3:69844046-69844065 MS.gene041681:intron 35.0%
TAGGGTTGTTCTATTTGATG+TGG + chr6.3:69843009-69843028 MS.gene041681:intron 35.0%
TCAATATTTCTCGCTGAATC+AGG - chr6.3:69844579-69844598 None:intergenic 35.0%
TCAGAGTATGTTTGGATGAA+CGG - chr6.3:69842850-69842869 None:intergenic 35.0%
TGATTCAGCGAGAAATATTG+AGG + chr6.3:69844578-69844597 MS.gene041681:CDS 35.0%
TGCAGCATTTCTTAAACTAC+AGG - chr6.3:69843306-69843325 None:intergenic 35.0%
TGTCAAATTATTAGCGGTGT+CGG - chr6.3:69843880-69843899 None:intergenic 35.0%
TTAGTGCATGTTTGGTATCA+CGG + chr6.3:69842451-69842470 MS.gene041681:intron 35.0%
TTTCAATAAGCCTTTGCCTT+CGG - chr6.3:69845658-69845677 None:intergenic 35.0%
TTTCTATTCTTCACCATCTC+AGG + chr6.3:69845773-69845792 MS.gene041681:intron 35.0%
TTTCTTCATGTGTGATACCT+AGG - chr6.3:69843467-69843486 None:intergenic 35.0%
! ACATCTCTAAAAACAGGAAC+CGG - chr6.3:69842663-69842682 None:intergenic 35.0%
! GTAGTAACGTCTTCTGATTT+TGG + chr6.3:69843384-69843403 MS.gene041681:intron 35.0%
! TAAACAGATGTGTCTGAGAT+GGG - chr6.3:69845071-69845090 None:intergenic 35.0%
! TGGATGACATCTCTAAAAAC+AGG - chr6.3:69842669-69842688 None:intergenic 35.0%
!! ATTTTGATGATTGTAGGAGG+AGG + chr6.3:69842275-69842294 MS.gene041681:intron 35.0%
!!! CTTAATGAAGAGTTTTTGGC+TGG + chr6.3:69845347-69845366 MS.gene041681:intron 35.0%
!!! TTTTATCTGGGACTAGTTCT+TGG + chr6.3:69845438-69845457 MS.gene041681:intron 35.0%
!!! TTTTTGGCTGGTTAATCTAG+TGG + chr6.3:69845359-69845378 MS.gene041681:intron 35.0%
AAACCAATGAACCTATGAGC+CGG - chr6.3:69844130-69844149 None:intergenic 40.0%
AGAATCAGGATCACCTTCTT+AGG - chr6.3:69842696-69842715 None:intergenic 40.0%
AGTCAAGTAGATCACCACAA+TGG - chr6.3:69843154-69843173 None:intergenic 40.0%
ATGTTTGAATAGGCCAATGG+AGG + chr6.3:69842623-69842642 MS.gene041681:intron 40.0%
CAGGATAAAGAATTGCTCCA+TGG - chr6.3:69843523-69843542 None:intergenic 40.0%
CATCTCAGACACATCTGTTT+AGG + chr6.3:69845070-69845089 MS.gene041681:intron 40.0%
CCAAGATTTGAATCCCATTG+TGG + chr6.3:69843137-69843156 MS.gene041681:CDS 40.0%
CCACAATGGGATTCAAATCT+TGG - chr6.3:69843140-69843159 None:intergenic 40.0%
CTAAACAGATGTGTCTGAGA+TGG - chr6.3:69845072-69845091 None:intergenic 40.0%
CTAGAGTAGGAAGAACTTGT+AGG + chr6.3:69844797-69844816 MS.gene041681:intron 40.0%
GAAACAAAGAGAAGCAGCAA+AGG + chr6.3:69844626-69844645 MS.gene041681:CDS 40.0%
GCTGCTCAGTTAGATTAAGT+TGG + chr6.3:69844874-69844893 MS.gene041681:intron 40.0%
GCTGGTTAATCTAGTGGTAA+AGG + chr6.3:69845365-69845384 MS.gene041681:intron 40.0%
GGAACCATATGTATGAAGCA+CGG - chr6.3:69844001-69844020 None:intergenic 40.0%
GTCAAGTAGATCACCACAAT+GGG - chr6.3:69843153-69843172 None:intergenic 40.0%
GTGGGTTTGCAAAATTACGA+TGG + chr6.3:69842473-69842492 MS.gene041681:intron 40.0%
GTTATCATGAAGCACGGATA+TGG + chr6.3:69843826-69843845 MS.gene041681:intron 40.0%
TGATCTTCCTGCTGAAAGAA+AGG + chr6.3:69844284-69844303 MS.gene041681:CDS 40.0%
TTCCAGTGAAATCCAGTACA+AGG + chr6.3:69842107-69842126 MS.gene041681:CDS 40.0%
TTGTCAACAGGTCTAGCATA+TGG - chr6.3:69845870-69845889 None:intergenic 40.0%
! ACGATGGTTCACCTTGATTT+TGG + chr6.3:69842489-69842508 MS.gene041681:intron 40.0%
! GACATGTGTCGTACAAGTTT+TGG - chr6.3:69844978-69844997 None:intergenic 40.0%
! GCATGCCTTCAACAATTTTC+AGG + chr6.3:69843103-69843122 MS.gene041681:intron 40.0%
! GGCAGAGGTAGTACTATTTT+AGG + chr6.3:69845408-69845427 MS.gene041681:intron 40.0%
! TATGAAGCACGGACACTTTT+TGG - chr6.3:69843990-69844009 None:intergenic 40.0%
! TGTTTTCAGCTGAAATCCGA+AGG + chr6.3:69845639-69845658 MS.gene041681:intron 40.0%
!! AGCATGGTACATTTTGAGTC+AGG - chr6.3:69843542-69843561 None:intergenic 40.0%
!! GATAAGCTTTAGGTAGTTGC+AGG + chr6.3:69845387-69845406 MS.gene041681:intron 40.0%
!!! CATGCGTTTTTCAAGAAACG+TGG + chr6.3:69842366-69842385 MS.gene041681:CDS 40.0%
ACAAAGAGAAGCAGCAAAGG+AGG + chr6.3:69844629-69844648 MS.gene041681:CDS 45.0%
ACTTGCAGGCACACACATAA+TGG - chr6.3:69843769-69843788 None:intergenic 45.0%
AGAACAACGAACACATCCAG+AGG - chr6.3:69843193-69843212 None:intergenic 45.0%
ATATGGACACGGACACAACA+CGG + chr6.3:69843843-69843862 MS.gene041681:intron 45.0%
ATCTGACAGAGTCTAACGCA+CGG - chr6.3:69843568-69843587 None:intergenic 45.0%
ATGAAGCACGGATATGGACA+CGG + chr6.3:69843832-69843851 MS.gene041681:intron 45.0%
CAAAGAGAAGCAGCAAAGGA+GGG + chr6.3:69844630-69844649 MS.gene041681:CDS 45.0%
CACATAGTTTCACAGACACG+AGG - chr6.3:69842585-69842604 None:intergenic 45.0%
CAGTATCACTCTCCTTGTAC+TGG - chr6.3:69842122-69842141 None:intergenic 45.0%
CATGGAGCCTAACCATGATT+CGG - chr6.3:69842759-69842778 None:intergenic 45.0%
CCAAGATCATGCTTGAGTCT+AGG - chr6.3:69843708-69843727 None:intergenic 45.0%
CCTAGACTCAAGCATGATCT+TGG + chr6.3:69843705-69843724 MS.gene041681:intron 45.0%
CTAATCGGTATGTTCACCTC+TGG + chr6.3:69843174-69843193 MS.gene041681:intron 45.0%
CTATGAAGCACAGACACGTA+TGG + chr6.3:69844932-69844951 MS.gene041681:intron 45.0%
CTCCTTGTACTGGATTTCAC+TGG - chr6.3:69842112-69842131 None:intergenic 45.0%
GAGGAAGAGAAATGCTGCAA+AGG + chr6.3:69845830-69845849 MS.gene041681:CDS 45.0%
GCACAGACACGTATGGAATT+AGG + chr6.3:69844939-69844958 MS.gene041681:intron 45.0%
GGAGGAGGAAGAGATAATGA+AGG + chr6.3:69842290-69842309 MS.gene041681:intron 45.0%
GTACGGTCGATAGTTTGAAC+CGG + chr6.3:69844108-69844127 MS.gene041681:intron 45.0%
GTGCGTCTAAATTGAGCTCT+TGG - chr6.3:69845226-69845245 None:intergenic 45.0%
GTGTCCGTGCTTCATACATA+TGG + chr6.3:69843994-69844013 MS.gene041681:intron 45.0%
TACTGGATTTCACTGGAACC+TGG - chr6.3:69842105-69842124 None:intergenic 45.0%
TCCAGGCAGTACCTACTTAA+AGG - chr6.3:69844060-69844079 None:intergenic 45.0%
TCTATTTCACTTCTGCGCTC+TGG - chr6.3:69844429-69844448 None:intergenic 45.0%
TGCATGTTTGGTATCACGGT+GGG + chr6.3:69842455-69842474 MS.gene041681:intron 45.0%
TGTCATCCATGCTCCTAAGA+AGG + chr6.3:69842680-69842699 MS.gene041681:CDS 45.0%
TTGCAGAACGCTCGATCAAA+CGG + chr6.3:69842339-69842358 MS.gene041681:CDS 45.0%
! AGTTTTCCAGATGCCTGAGA+TGG - chr6.3:69845789-69845808 None:intergenic 45.0%
! ATATGTGTCAGGTGTCGTGT+CGG + chr6.3:69843923-69843942 MS.gene041681:intron 45.0%
! GATAGTTTGAACCGGCTCAT+AGG + chr6.3:69844116-69844135 MS.gene041681:intron 45.0%
! TCCTTTAAGTAGGTACTGCC+TGG + chr6.3:69844056-69844075 MS.gene041681:intron 45.0%
! TTCTTGCTACTAGCCTCCAT+TGG - chr6.3:69842639-69842658 None:intergenic 45.0%
!! AATTTTCAAGCAGGCACTCC+AGG - chr6.3:69844077-69844096 None:intergenic 45.0%
!! GCACGGACACTTTTTGGATT+AGG - chr6.3:69843984-69844003 None:intergenic 45.0%
!!! GTTTTGGTTGGCATTGAGAC+TGG + chr6.3:69842733-69842752 MS.gene041681:intron 45.0%
!!! TTGAGGCAGAGATTTTGGCT+AGG + chr6.3:69844595-69844614 MS.gene041681:CDS 45.0%
!!! TAAAAATATTCAAATTTTTA+GGG + chr6.3:69842991-69843010 MS.gene041681:intron 5.0%
!!! TTAAAAATATTCAAATTTTT+AGG + chr6.3:69842990-69843009 MS.gene041681:intron 5.0%
AAGCTCGGAAGTGGAATTCC+AGG + chr6.3:69842084-69842103 MS.gene041681:CDS 50.0%
CAGCTGAAATCCGAAGGCAA+AGG + chr6.3:69845645-69845664 MS.gene041681:intron 50.0%
CTTTAGGTAGTTGCAGGCAG+AGG + chr6.3:69845393-69845412 MS.gene041681:intron 50.0%
GGATCACCTTCTTAGGAGCA+TGG - chr6.3:69842689-69842708 None:intergenic 50.0%
GTCTAACGCACGGTAAAGCA+TGG - chr6.3:69843558-69843577 None:intergenic 50.0%
GTGCATGTTTGGTATCACGG+TGG + chr6.3:69842454-69842473 MS.gene041681:intron 50.0%
TCTTCACCATCTCAGGCATC+TGG + chr6.3:69845780-69845799 MS.gene041681:intron 50.0%
! AGACTGGCCGAATCATGGTT+AGG + chr6.3:69842749-69842768 MS.gene041681:intron 50.0%
! CATTGAGACTGGCCGAATCA+TGG + chr6.3:69842744-69842763 MS.gene041681:intron 50.0%
! GAACCGGCTCATAGGTTCAT+TGG + chr6.3:69844124-69844143 MS.gene041681:intron 50.0%
GGAGGCTAGTAGCAAGAAGC+CGG + chr6.3:69842641-69842660 MS.gene041681:CDS 55.0%
! GTCAGGTGTCGTGTCGGTGT+CGG + chr6.3:69843929-69843948 MS.gene041681:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 69842078 69845896 69842078 ID=MS.gene041681
chr6.3 mRNA 69842078 69845896 69842078 ID=MS.gene041681.t1;Parent=MS.gene041681
chr6.3 exon 69842078 69842176 69842078 ID=MS.gene041681.t1.exon1;Parent=MS.gene041681.t1
chr6.3 CDS 69842078 69842176 69842078 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69842291 69842424 69842291 ID=MS.gene041681.t1.exon2;Parent=MS.gene041681.t1
chr6.3 CDS 69842291 69842424 69842291 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69842635 69842701 69842635 ID=MS.gene041681.t1.exon3;Parent=MS.gene041681.t1
chr6.3 CDS 69842635 69842701 69842635 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69843125 69843180 69843125 ID=MS.gene041681.t1.exon4;Parent=MS.gene041681.t1
chr6.3 CDS 69843125 69843180 69843125 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69844251 69844305 69844251 ID=MS.gene041681.t1.exon5;Parent=MS.gene041681.t1
chr6.3 CDS 69844251 69844305 69844251 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69844393 69844467 69844393 ID=MS.gene041681.t1.exon6;Parent=MS.gene041681.t1
chr6.3 CDS 69844393 69844467 69844393 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69844561 69844678 69844561 ID=MS.gene041681.t1.exon7;Parent=MS.gene041681.t1
chr6.3 CDS 69844561 69844678 69844561 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69845648 69845694 69845648 ID=MS.gene041681.t1.exon8;Parent=MS.gene041681.t1
chr6.3 CDS 69845648 69845694 69845648 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
chr6.3 exon 69845795 69845896 69845795 ID=MS.gene041681.t1.exon9;Parent=MS.gene041681.t1
chr6.3 CDS 69845795 69845896 69845795 ID=cds.MS.gene041681.t1;Parent=MS.gene041681.t1
Gene Sequence

>MS.gene041681

ATGAAGAAGCTCGGAAGTGGAATTCCAGGTTCCAGTGAAATCCAGTACAAGGAGAGTGATACTGACGAGTCAATGTCTTCTTCTAGCATATCAGATGATGTGAGTAACATCAAAAAATCATCATGTTATATACTTTTTAATAAAAAACTAACCATGTCATAGTAAGTTTGTAACTTTGTATTATTGTATTATTATTGATTTTGATGATTGTAGGAGGAGGAAGAGATAATGAAGGAGTTAGCTGATGTAACGTTTGAAGAATTGCAGAACGCTCGATCAAACGGAGATCATGCGTTTTTCAAGAAACGTGGAGAAGACAAGAAGTTAAAAAGGCTTAATAAGAATAGGTGTTTTATTTGTATCATAGTAATAGTTAGTGCATGTTTGGTATCACGGTGGGTTTGCAAAATTACGATGGTTCACCTTGATTTTGGCAAAGTTACTCTGATACCAAACATGTACTCAATTGATTGTTTGACAAAGTGGTTTTCATTTTTTGCTAATCCTCGTGTCTGTGAAACTATGTGCAAGTAAAATAATTTCTAATGTTTGAATAGGCCAATGGAGGCTAGTAGCAAGAAGCCGGTTCCTGTTTTTAGAGATGTCATCCATGCTCCTAAGAAGGTGATCCTGATTCTTGACACATTGATTTCATGTTTTGGTTGGCATTGAGACTGGCCGAATCATGGTTAGGCTCCATGATTTTGACAAAACCTAATCTTTGTTGCTTCTACAAAATTATGGTGTCACCGTGATTTTATCAAACTCACCGTTCATCCAAACATACTCTGAATAGTTTCAAATCAAAATAGTTTCATGGAAGCCATTAGCTTTTATTTTTGGGGGTTATATTTTTGACTATTTTCCCTATAAACCAATTGCTCATTGTTTACTCTACAACTGCGCACGCACTTAAAAATATTCAAATTTTTAGGGTTGTTCTATTTGATGTGGAAACTCATGTTGAATTTGAATGTAAATGTGATATTCTGTTTTATATATGTGGATGATTTTTGATGAGTTATGCATGCCTTCAACAATTTTCAGGTTGTATGTGATCCAAGATTTGAATCCCATTGTGGTGATCTACTTGACTCTAATCGGTATGTTCACCTCTGGATGTGTTCGTTGTTCTACCTTTTTCTTGTTGTGTTAGAATCTGTCTGATAATATAGTATTCTTTGCTGGTGTTAGAATCTGTCTGATAACATGATATGATCTTTATCCTGTAGTTTAAGAAATGCTGCAGTTTTTTATTTGCAATCATCATATCAATTTTAAGCATTTTCATATTGTAGTAACGTCTGTAGTAACGTCTTCTGATTTTGGTTGTATTTTAGAGCATGTGCGCCCAACGTTTCCTCAAGTATTTTAAATTATATAAGACCTAGGTATCACACATGAAGAAAACAACTTATTGAGAATGTGTTAAAGGCTTAAATCCATGGAGCAATTCTTTATCCTGACTCAAAATGTACCATGCTTTACCGTGCGTTAGACTCTGTCAGATAATATTGTAGAATCTTTATAGCGACAATTATTTTTAGTGCATACCAAAAAAGCATCAAATGATTTATAAATCTCCAATCCATACTATGATTTAGACAATTTTATCATTCATTAATCACCTAGACTCAAGCATGATCTTGGAGCAACTTTTACGATATTATGTTGGTGTTGGTGTCTTACCATTATGTGTGTGCCTGCAAGTATGTTACTTATTGAGTGGCATTTGGTATCTTTATTTGGTTATCATGAAGCACGGATATGGACACGGACACAACACGGACACTGACACGCCGACACCGCTAATAATTTGACAAAATCACATAATTAAGTGTAATTATATGTGTCAGGTGTCGTGTCGGTGTCGGACACGACACATGTTCGACACTAACACACGCCTAATCCAAAAAGTGTCCGTGCTTCATACATATGGTTCCTTTTTGTTCTTGGTTTGAGTTTCTGTAATGTCGTGTCCTTTAAGTAGGTACTGCCTGGAGTGCCTGCTTGAAAATTAGTATTCAATTGTACGGTCGATAGTTTGAACCGGCTCATAGGTTCATTGGTTTTTTAGTATACAATGTTTACTTATATCTGCATTGTACTTCGACTATGCTTTTGATGTTACTATTCTGTGCAGCACTAATAATATAAGTACTTGGACTTACAGGTTTAGAAAGAAATATAAGTTTTTATACGAAAATGATCTTCCTGCTGAAAGAAAGGTAGTATTCTTGCAGATTGTAACAGCAAAATTTCATCTGTAACTTATTTTGTGACTTTCGTCTAATGACATTTTCTGGATAAAACAGACTCTTAAGAGGGAATTGAAAAAATGTAAAGATCCAGAGCGCAGAAGTGAAATAGAAGAACTCGTATCATGGATTGTAAGTATTTATCATCTTTTTCGAAGTTTGTCTTCTTCGTCCAGAAGTGTGACATTTATTAGTTTTCTTGATCACATTTACATCTCTTTTCAGGATAAACAGTTGAAGCCTGATTCAGCGAGAAATATTGAGGCAGAGATTTTGGCTAGGCACAAGAAGAAACAAAGAGAAGCAGCAAAGGAGGGAAGACGTCCTTTTTATCTCAAGAAATGTAATTTTTCTGTTCCTTCCTTATCATTTTATTTCCGTTTTCTCTCACTTTAGATATGTTAGAAATGCAATCTCTGCTTATGTTAATTTTATCTCCCTTTTCTTTCACTTTCTATATGCTAGAGTAGGAAGAACTTGTAGGTTTACTATAGGAGGAAGATGAAAAATAAGTTTTAAAGGTGGCATTTAATTAGTTGCTGCTCAGTTAGATTAAGTTGGGAAATCAACTGAAGTTTGTTACTCTGTTAGCATTACTATGAAGCACAGACACGTATGGAATTAGGTGTATCGCAGTGTCCAAAACTTGTACGACACATGTCTGACAACTCCGAAACATTATCTTAAAAAATAACATTTTTTTTCTTCCACACATTTTACACATTCTTTAGACCCATCTCAGACACATCTGTTTAGGTTAAAATGCGTCGAAAGCAATGATGATCATTGAGGGAGTTCAAGACATTAAGTTTGATTACATCATTTTTAGCTTTTTGGCTGTTCCATATTTTTTTTAGCTTTTCGGTATGAATGAATACGAAAGACTGCCAAGAGCTCAATTTAGACGCACATATATATTTTGATTCTCAATTTAGATCTTTTATAAGTGTTGTGTCATGTGTCTGTTATTCATAAGTTAGCAGTAAGTTTTTTCTTTCTATGAATACCTCTCTTAATGAAGAGTTTTTGGCTGGTTAATCTAGTGGTAAAGGATAAGCTTTAGGTAGTTGCAGGCAGAGGTAGTACTATTTTAGGTGTTTTTTTTTATCTGGGACTAGTTCTTGGATATCATCTTTGTTCACTTGAATTATTTCCCCGTTTAGATTTTTGAATTCGATATACAATTCTTTGGCAATTCATTGAATTTATGGTTAAACGTCAAACAAATGAACTGACAAATATGTCAAACAGACAAATTTGAATATAATGAAAAATCGCCTACATATAATGACATTTTTTTATTTGTTTTCAGCTGAAATCCGAAGGCAAAGGCTTATTGAAAAATATAAACATCTCAAGGTATGTATCTTAGAACATCTCTATGTTCTCCCTTCACCTTGTATTCTGTCATTTATTGCTTGTCTTTTCCATTAATTCTTTCTATTCTTCACCATCTCAGGCATCTGGAAAACTTGATTCATATATTGAGAAAAGGAGGAAGAGAAATGCTGCAAAGGACCATAGATTTATGCCATATGCTAGACCTGTTGACAACGAATAG
Protein sequence

>MS.gene041681.t1

MKKLGSGIPGSSEIQYKESDTDESMSSSSISDDEEEEIMKELADVTFEELQNARSNGDHAFFKKRGEDKKLKRLNKNRPMEASSKKPVPVFRDVIHAPKKVVCDPRFESHCGDLLDSNRFRKKYKFLYENDLPAERKTLKRELKKCKDPERRSEIEELVSWIDKQLKPDSARNIEAEILARHKKKQREAAKEGRRPFYLKKSEIRRQRLIEKYKHLKASGKLDSYIEKRRKRNAAKDHRFMPYARPVDNE