AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene046374

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046374.t1 MTR_8g027995 93.441 808 19 3 1 774 214 1021 0.0 1492
MS.gene046374.t1 MTR_7g080920 58.101 790 251 16 1 774 236 961 0.0 729
MS.gene046374.t1 MTR_4g087890 67.780 419 117 5 371 774 429 844 0.0 556
MS.gene046374.t1 MTR_5g015690 62.228 413 141 7 370 774 455 860 1.24e-164 500
MS.gene046374.t1 MTR_4g095780 60.993 423 147 7 363 774 394 809 7.56e-160 486
MS.gene046374.t1 MTR_1g083260 53.483 402 172 7 379 774 497 889 8.96e-130 409
MS.gene046374.t1 MTR_4g045670 48.618 434 203 8 370 793 382 805 1.13e-117 376
MS.gene046374.t1 MTR_4g081410 48.668 413 197 6 370 774 521 926 7.69e-116 374
MS.gene046374.t1 MTR_4g081410 48.668 413 197 6 370 774 527 932 1.01e-115 374
MS.gene046374.t1 MTR_2g082190 48.418 411 194 7 377 775 393 797 2.02e-113 365
MS.gene046374.t1 MTR_2g089040 45.874 412 200 8 377 775 375 776 2.65e-103 338
MS.gene046374.t1 MTR_5g036540 40.698 430 217 11 376 776 360 780 6.51e-90 301
MS.gene046374.t1 MTR_8g062830 36.673 499 255 15 305 774 255 721 8.09e-77 264
MS.gene046374.t1 MTR_3g078623 38.119 404 232 8 376 773 239 630 7.02e-76 260
MS.gene046374.t1 MTR_4g131020 27.200 375 233 8 379 746 792 1133 9.74e-34 140
MS.gene046374.t1 MTR_4g131020 27.200 375 233 8 379 746 792 1133 1.70e-33 139
MS.gene046374.t1 MTR_4g109040 26.440 382 233 10 380 749 837 1182 1.16e-32 137
MS.gene046374.t1 MTR_4g109040 26.440 382 233 10 380 749 1171 1516 4.65e-32 135
MS.gene046374.t1 MTR_1g013800 24.862 362 227 7 374 725 1359 1685 5.62e-30 128
MS.gene046374.t1 MTR_3g037080 30.000 250 161 5 380 619 1509 1754 1.71e-28 124
MS.gene046374.t1 MTR_5g026645 24.242 363 226 8 375 725 788 1113 1.03e-25 114
MS.gene046374.t1 MTR_5g026645 24.242 363 226 8 375 725 803 1128 1.04e-25 114
MS.gene046374.t1 MTR_5g026645 24.242 363 226 8 375 725 629 954 1.35e-25 114
MS.gene046374.t1 MTR_2g086990 70.175 57 17 0 407 463 35 91 5.85e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046374.t1 AT3G25500 73.094 446 103 5 337 774 551 987 0.0 645
MS.gene046374.t1 AT2G43800 62.410 415 135 6 370 774 438 841 1.10e-172 521
MS.gene046374.t1 AT5G67470 59.725 437 156 5 346 774 420 844 8.39e-164 498
MS.gene046374.t1 AT3G05470 51.825 411 169 8 378 774 469 864 4.29e-125 396
MS.gene046374.t1 AT5G54650 45.114 481 224 10 308 776 391 843 5.24e-112 362
MS.gene046374.t1 AT5G54650 45.114 481 224 10 308 776 391 843 5.24e-112 362
MS.gene046374.t1 AT5G48360 45.708 431 169 9 346 774 409 776 1.71e-105 342
MS.gene046374.t1 AT3G07540 41.995 431 201 6 347 774 446 830 9.70e-87 293
MS.gene046374.t1 AT1G70140 39.041 438 225 10 361 774 290 709 1.61e-83 283
MS.gene046374.t1 AT1G59910 37.412 425 233 11 371 777 454 863 5.32e-78 271
MS.gene046374.t1 AT1G24150 42.922 219 104 6 379 587 312 519 8.23e-44 167
MS.gene046374.t1 AT5G07740 26.121 379 235 7 377 746 1244 1586 2.72e-33 139
MS.gene046374.t1 AT5G07740 26.121 379 235 7 377 746 1222 1564 2.87e-33 139
MS.gene046374.t1 AT3G25493 65.000 80 28 0 696 775 31 110 1.65e-29 115
MS.gene046374.t1 AT5G07740 24.939 413 231 10 377 746 1244 1620 9.27e-29 124
MS.gene046374.t1 AT1G31810 24.074 378 244 7 377 746 816 1158 2.14e-28 123
MS.gene046374.t1 AT1G31810 24.074 378 244 7 377 746 816 1158 2.14e-28 123
MS.gene046374.t1 AT5G58160 23.325 403 263 9 379 773 838 1202 3.09e-27 119
MS.gene046374.t1 AT5G07770 23.861 373 236 7 380 746 199 529 6.89e-26 114
MS.gene046374.t1 AT5G07770 23.861 373 236 7 380 746 192 522 9.35e-26 114
MS.gene046374.t1 AT5G07770 24.403 377 229 9 380 746 58 388 4.09e-25 111
MS.gene046374.t1 AT5G07770 23.611 360 220 8 397 746 181 495 2.56e-22 103
MS.gene046374.t1 AT3G32400 23.038 395 246 10 342 725 69 416 2.61e-22 102
MS.gene046374.t1 AT5G07760 23.684 304 199 5 444 746 523 794 1.93e-21 100
MS.gene046374.t1 AT5G07760 23.684 304 199 5 444 746 523 794 2.16e-21 100
MS.gene046374.t1 AT2G25050 21.814 408 248 10 374 746 739 1110 1.37e-20 98.2
MS.gene046374.t1 AT5G07650 23.510 302 200 4 445 746 470 740 4.19e-19 92.8
MS.gene046374.t1 AT5G07780 22.680 388 238 11 376 746 74 416 3.22e-17 85.9
MS.gene046374.t1 AT3G32400 26.493 268 174 8 355 605 65 326 2.79e-16 83.2
MS.gene046374.t1 AT5G07650 22.393 326 198 5 445 746 470 764 5.64e-16 82.8

Find 186 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGCTGGTTGACGTTAA+AGG 0.220743 8.3:+65714394 MS.gene046374:CDS
CGGGATTTCAGCTGAGAATT+TGG 0.224665 8.3:+65711607 MS.gene046374:CDS
CTCCACTTCAGATTCAAAAT+TGG 0.253331 8.3:-65714116 None:intergenic
ACTGGAGAAACCAATGTATT+TGG 0.257163 8.3:-65712602 None:intergenic
GGTGGTGGTGGCGGTGGTGG+TGG 0.265460 8.3:-65712440 None:intergenic
CACCGCCGCAACCAGCTTCT+AGG 0.283505 8.3:+65712273 MS.gene046374:CDS
GGTGGTGGTGGTGGCGGTGG+TGG 0.297185 8.3:-65712443 None:intergenic
ACTTCTTAACAGTTCTTGAT+AGG 0.298082 8.3:+65714896 MS.gene046374:CDS
CTAGAGGCTGTGCTCAAAAC+TGG 0.304797 8.3:+65714310 MS.gene046374:CDS
ACTCTACTAGAAGGTGTAAT+TGG 0.308943 8.3:-65712407 None:intergenic
CTAGAAGCTGGTTGCGGCGG+TGG 0.314053 8.3:-65712272 None:intergenic
ATTCAACAATTGGTGTTAAC+AGG 0.316607 8.3:-65712316 None:intergenic
ATGCTTCCACAACCACTTCC+TGG 0.318071 8.3:+65714997 MS.gene046374:CDS
TTGCGCTCTTCTTCCTTGCT+TGG 0.329564 8.3:-65713977 None:intergenic
CAATGCAAAGGCTTCAACTT+TGG 0.331536 8.3:-65712668 None:intergenic
ACTACCATGAAGATTCTAAA+TGG 0.334807 8.3:-65714868 None:intergenic
GGAGTAGCATCCTTTGGTTT+TGG 0.338452 8.3:-65713037 None:intergenic
ACGGAAATGGGTCGGGTTCT+CGG 0.342408 8.3:+65711573 MS.gene046374:CDS
AGAGGTTCTTTAAACGGAAA+TGG 0.343572 8.3:+65711560 MS.gene046374:CDS
GGTGGTGGCGGTGGTGGTGG+AGG 0.346800 8.3:-65712437 None:intergenic
TTGGTCAACCGGTGGTTTGT+AGG 0.347205 8.3:+65712531 MS.gene046374:CDS
TCAGCAGAACCGAGTTTGTT+TGG 0.349669 8.3:-65714025 None:intergenic
TAGAAGCTGGTTGCGGCGGT+GGG 0.356702 8.3:-65712271 None:intergenic
TCATCCATTTAGAATCTTCA+TGG 0.368202 8.3:+65714864 MS.gene046374:CDS
AACAACTCCTGTTGGTCAAC+CGG 0.373070 8.3:+65712520 MS.gene046374:CDS
TTCCGATCGTGAAATGGTTT+GGG 0.375123 8.3:+65712709 MS.gene046374:CDS
AGCATAGGGTTCACTGGAAC+AGG 0.375806 8.3:-65714979 None:intergenic
TCTCCAAGAGGTTCTTTAAA+CGG 0.382395 8.3:+65711554 MS.gene046374:CDS
GTTCCGATCGTGAAATGGTT+TGG 0.383673 8.3:+65712708 MS.gene046374:CDS
ACAGTTGGTGATTCAACAAT+TGG 0.390675 8.3:-65712326 None:intergenic
CATTGCTTCCGCTGCTTCAT+CGG 0.394401 8.3:-65712482 None:intergenic
AAACTAGGTGGCAACTCAAC+TGG 0.402014 8.3:-65712620 None:intergenic
TAACAAACTTTCAAGTAGTT+CGG 0.403932 8.3:-65713945 None:intergenic
GAAGCTGGTTGCGGCGGTGG+GGG 0.414519 8.3:-65712269 None:intergenic
GCAAGTGGAGGTGGTGGTGG+TGG 0.414860 8.3:-65712455 None:intergenic
AGTGGAGGTGGTGGTGGTGG+TGG 0.415651 8.3:-65712452 None:intergenic
CAGAATTAAAACCTCCTTCA+AGG 0.417513 8.3:+65712558 MS.gene046374:CDS
AACCATTTCACGATCGGAAC+TGG 0.417864 8.3:-65712705 None:intergenic
CCACTACTTGTTAGTGAAGT+TGG 0.417990 8.3:+65711797 MS.gene046374:CDS
CAACCACTTCCTGGATTACA+TGG 0.421232 8.3:+65715006 MS.gene046374:CDS
TTCATCTTCTTCTGCTTCAC+CGG 0.429921 8.3:+65711673 MS.gene046374:CDS
AGAAGCTGGTTGCGGCGGTG+GGG 0.432904 8.3:-65712270 None:intergenic
AATGGTGGTGCTAATGTTAC+AGG 0.433893 8.3:+65711503 MS.gene046374:CDS
GAACGTGGAGTAGCATCCTT+TGG 0.434256 8.3:-65713043 None:intergenic
AAAGTTGAAGCCTTTGCATT+GGG 0.434876 8.3:+65712670 MS.gene046374:CDS
GGCGGTAACGGCTGTAGCTC+CGG 0.439758 8.3:-65711446 None:intergenic
CTTCTTCACATTCGCTAGAT+CGG 0.443176 8.3:-65714581 None:intergenic
GGCGTTTACTTTGCCATCGC+CGG 0.443924 8.3:+65712082 MS.gene046374:CDS
GGAAGAAGAGCGCAAATTGA+AGG 0.444590 8.3:+65713985 MS.gene046374:CDS
ACAATTGGTGTTAACAGGTC+AGG 0.445562 8.3:-65712311 None:intergenic
TGAATCACAAAAGGCCTTGA+AGG 0.448329 8.3:-65712572 None:intergenic
GAGGTTCTTTAAACGGAAAT+GGG 0.448607 8.3:+65711561 MS.gene046374:CDS
AAATGGTTTGGGATCATTTG+AGG 0.449038 8.3:+65712720 MS.gene046374:CDS
GGCCAGTTCCGATCGTGAAA+TGG 0.449603 8.3:+65712703 MS.gene046374:CDS
ATTGCAAGTGGAGGTGGTGG+TGG 0.449780 8.3:-65712458 None:intergenic
TCTTTAAACGGAAATGGGTC+GGG 0.449904 8.3:+65711566 MS.gene046374:CDS
GTAATTGGTGTTGAAAGAAC+AGG 0.452574 8.3:-65712392 None:intergenic
ATGAATCTTCATAGCAATCA+TGG 0.453942 8.3:+65712119 MS.gene046374:CDS
ATTGAATCAGACGGCGGGAT+CGG 0.458070 8.3:+65714687 MS.gene046374:CDS
AATCAATGAACAAGTTTATG+AGG 0.460163 8.3:+65714734 MS.gene046374:CDS
CAAAGTTGAAGCCTTTGCAT+TGG 0.465268 8.3:+65712669 MS.gene046374:CDS
AAGACCAAGTCTTCTGCACT+TGG 0.465976 8.3:-65714536 None:intergenic
TTTCCGTTTAAAGAACCTCT+TGG 0.468902 8.3:-65711557 None:intergenic
GAAGTCTCTAAACTTTCAAA+AGG 0.468986 8.3:+65714637 MS.gene046374:CDS
CAAACCACCGGTTGACCAAC+AGG 0.469210 8.3:-65712527 None:intergenic
TGTCGCACAAGCGGCGGTAA+CGG 0.472296 8.3:-65711458 None:intergenic
AATGATAGAAACATTATTTG+TGG 0.478939 8.3:+65713000 MS.gene046374:CDS
TCTGAAACATCAGAAATTGT+TGG 0.480351 8.3:-65712155 None:intergenic
TTCTTTAAACGGAAATGGGT+CGG 0.480385 8.3:+65711565 MS.gene046374:CDS
GAAAGAAGCGTTGGTGAAAG+TGG 0.482301 8.3:-65712209 None:intergenic
GGAGCTGAAACTCTACTAGA+AGG 0.484946 8.3:-65712416 None:intergenic
AACCAGCTTCTAGGAAGCAT+TGG 0.485263 8.3:+65712282 MS.gene046374:CDS
TTCGATCAGTCTTTCTCCAC+CGG 0.485479 8.3:+65711706 MS.gene046374:CDS
GGAGGAGTTTGAATCACAAA+AGG 0.486460 8.3:-65712581 None:intergenic
CAAATTCTCAGCTGAAATCC+CGG 0.495530 8.3:-65711606 None:intergenic
AAGCTCGTTGTCGTCGTCAC+GGG 0.496123 8.3:+65711823 MS.gene046374:CDS
ACTTCGAGCGGTTAATGTTA+CGG 0.497276 8.3:+65713135 MS.gene046374:CDS
ATCGGCATTGCAAGTGGAGG+TGG 0.498071 8.3:-65712464 None:intergenic
TGTAATCCAGGAAGTGGTTG+TGG 0.499250 8.3:-65715003 None:intergenic
CTTCTTAACAGTTCTTGATA+GGG 0.499816 8.3:+65714897 MS.gene046374:CDS
CGATCTAGCGAATGTGAAGA+AGG 0.502743 8.3:+65714582 MS.gene046374:CDS
ACCCTATCCTCGTGACTTGG+AGG 0.502964 8.3:-65713076 None:intergenic
GGTTGACCAACAGGAGTTGT+TGG 0.504807 8.3:-65712518 None:intergenic
AAACCCTTACTTAAAAGAGC+TGG 0.505939 8.3:-65712744 None:intergenic
GATTTCAGCTGAGAATTTGG+TGG 0.506846 8.3:+65711610 MS.gene046374:CDS
ACCAGCTTCTAGGAAGCATT+GGG 0.509100 8.3:+65712283 MS.gene046374:CDS
ACTGGGAACCGCATGAATGT+TGG 0.511036 8.3:+65714328 MS.gene046374:CDS
TTTCCATGTAATCCAGGAAG+TGG 0.512207 8.3:-65715009 None:intergenic
AATACCCTATCCTCGTGACT+TGG 0.513434 8.3:-65713079 None:intergenic
TCTTCATTCTCCTCAAAACT+AGG 0.514159 8.3:-65712635 None:intergenic
AAGTCACGAGGATAGGGTAT+TGG 0.515638 8.3:+65713081 MS.gene046374:CDS
TCCCAATGCTTCCTAGAAGC+TGG 0.515895 8.3:-65712284 None:intergenic
GAAGCTCGTTGTCGTCGTCA+CGG 0.516519 8.3:+65711822 MS.gene046374:CDS
CGATGAAGCAGCGGAAGCAA+TGG 0.518498 8.3:+65712483 MS.gene046374:CDS
TCAAACTCCTCCAAATACAT+TGG 0.521809 8.3:+65712592 MS.gene046374:CDS
TGTCTCTTTCCATGTAATCC+AGG 0.522444 8.3:-65715015 None:intergenic
GGAAGTGGTTGTGGAAGCAT+AGG 0.525174 8.3:-65714994 None:intergenic
AGTAGTGGCTGTGTCGGTGC+CGG 0.525966 8.3:-65711782 None:intergenic
CAAGAAATCATTAGAACAGA+AGG 0.526066 8.3:+65714454 MS.gene046374:CDS
GATGCCAAGTGCAGAAGACT+TGG 0.528209 8.3:+65714532 MS.gene046374:CDS
TGTGGAAGCATAGGGTTCAC+TGG 0.528253 8.3:-65714985 None:intergenic
AGCGGTTAATGTTACGGTAG+AGG 0.530275 8.3:+65713141 MS.gene046374:CDS
CACCTCCAAGTCACGAGGAT+AGG 0.534967 8.3:+65713074 MS.gene046374:CDS
GCTTGATACACTTCTCAAGC+TGG 0.535039 8.3:+65714381 MS.gene046374:CDS
ACCTCCAAGTCACGAGGATA+GGG 0.535294 8.3:+65713075 MS.gene046374:CDS
AATGAACAAGTTTATGAGGA+TGG 0.535360 8.3:+65714738 MS.gene046374:CDS
CCATTCTGATTCAAAACAGT+TGG 0.535974 8.3:-65712341 None:intergenic
GATGAAGCAGCGGAAGCAAT+GGG 0.538647 8.3:+65712484 MS.gene046374:CDS
TGTGAAGAAGGCCGCGACAA+TGG 0.539842 8.3:+65714594 MS.gene046374:CDS
ACAGGTGTTACTGATACACT+TGG 0.542268 8.3:+65713920 MS.gene046374:intron
TGCTTCATCGGCATTGCAAG+TGG 0.544224 8.3:-65712470 None:intergenic
ATTTCAGCTGAGAATTTGGT+GGG 0.545866 8.3:+65711611 MS.gene046374:CDS
GGAGAAACCAATGTATTTGG+AGG 0.546058 8.3:-65712599 None:intergenic
TGTTTCTCAGAGAAAACAAT+GGG 0.549128 8.3:+65712364 MS.gene046374:CDS
CAATTTCTGATGTTTCAGAT+CGG 0.552148 8.3:+65712159 MS.gene046374:CDS
AATTGGGTGCAGGTTGAATG+AGG 0.552324 8.3:+65712976 MS.gene046374:intron
TGCTTCCTAGAAGCTGGTTG+CGG 0.553741 8.3:-65712278 None:intergenic
AGACGGTGACTTACATTCTC+CGG 0.553919 8.3:-65711909 None:intergenic
ACCAACGCTTCTTTCGTCGC+CGG 0.554767 8.3:+65712217 MS.gene046374:CDS
TTACCGCCGCTTGTGCGACA+AGG 0.555576 8.3:+65711461 MS.gene046374:CDS
GGCATTGCAAGTGGAGGTGG+TGG 0.556796 8.3:-65712461 None:intergenic
CTTTACCTCTAGAGTCTGAA+AGG 0.557119 8.3:-65714152 None:intergenic
GGAAGTGTGTGAAGCACTGT+TGG 0.558493 8.3:+65713162 MS.gene046374:CDS
ATCCCAAACCATTTCACGAT+CGG 0.564571 8.3:-65712711 None:intergenic
TAGAGGCTGTGCTCAAAACT+GGG 0.565113 8.3:+65714311 MS.gene046374:CDS
GTAAAATTGAATCAGACGGC+GGG 0.568789 8.3:+65714682 MS.gene046374:CDS
TCTTCATAGCAATCATGGGT+TGG 0.568916 8.3:+65712124 MS.gene046374:CDS
CTAGAAGCATTCGATAATCT+CGG 0.569242 8.3:-65712032 None:intergenic
GACTTCGCTCGTGAGGACTT+CGG 0.569518 8.3:-65714620 None:intergenic
TCGTCACTCAATGTTGCGCT+CGG 0.570493 8.3:-65714511 None:intergenic
ACCGGAAAATGTTGTAACTC+CGG 0.573138 8.3:+65711763 MS.gene046374:CDS
GTGCTCATTATATCTCTCTC+CGG 0.580272 8.3:-65712236 None:intergenic
ATCGAGCCTTGTCGCACAAG+CGG 0.581241 8.3:-65711467 None:intergenic
GTTTATGAGGATGGCTGAGG+AGG 0.581248 8.3:+65714747 MS.gene046374:CDS
TTCCTAGAAGCTGGTTGCGG+CGG 0.582477 8.3:-65712275 None:intergenic
TGATTCTGCAAATTTACGAA+CGG 0.583337 8.3:-65714716 None:intergenic
CTTTGCATTGGGATAAAGTG+AGG 0.585023 8.3:+65712681 MS.gene046374:CDS
AAGATCACAACCAAAATCAC+CGG 0.588402 8.3:+65711745 MS.gene046374:CDS
CAAGGCTCGATGTATGATGA+AGG 0.588795 8.3:+65711479 MS.gene046374:CDS
ATCATCGTCTTCTTCATCGT+CGG 0.589871 8.3:+65712061 MS.gene046374:CDS
CAAGTTTATGAGGATGGCTG+AGG 0.589924 8.3:+65714744 MS.gene046374:CDS
TCCGGCGACGAAAGAAGCGT+TGG 0.589989 8.3:-65712218 None:intergenic
AAAGAGACATTACAGTTCCT+CGG 0.592779 8.3:+65715029 MS.gene046374:CDS
CTAACAAGTAGTGGCTGTGT+CGG 0.598633 8.3:-65711788 None:intergenic
GTTTAGAGACTTCGCTCGTG+AGG 0.598816 8.3:-65714627 None:intergenic
CCAACAGGAGTTGTTGGAGA+AGG 0.599161 8.3:-65712512 None:intergenic
TATTGCTATCTTACTTCGAG+CGG 0.599343 8.3:+65713123 MS.gene046374:CDS
CCAACTTCACTAACAAGTAG+TGG 0.601777 8.3:-65711797 None:intergenic
CTCACTTTATCCCAATGCAA+AGG 0.604325 8.3:-65712680 None:intergenic
ATCACAAAAGGCCTTGAAGG+AGG 0.606239 8.3:-65712569 None:intergenic
TAAATCCTTTCAGACTCTAG+AGG 0.608370 8.3:+65714147 MS.gene046374:CDS
TCTCCAGAGAAAATCTTCGC+CGG 0.608455 8.3:+65711890 MS.gene046374:CDS
CTTCTTGGACTCAAACTCAC+CGG 0.609186 8.3:-65711725 None:intergenic
AATGTTCTTGAAGCTTCTAG+AGG 0.609255 8.3:+65714294 MS.gene046374:CDS
TGAATCTTCATAGCAATCAT+GGG 0.610122 8.3:+65712120 MS.gene046374:CDS
CCTTCTCCAACAACTCCTGT+TGG 0.612388 8.3:+65712512 MS.gene046374:CDS
TTTGCATTGGGATAAAGTGA+GGG 0.612685 8.3:+65712682 MS.gene046374:CDS
CTGATCGAATGAGAACGATC+CGG 0.614443 8.3:-65711692 None:intergenic
GAAGTGGTTGTGGAAGCATA+GGG 0.615204 8.3:-65714993 None:intergenic
GTGTGTGAAGCACTGTTGGA+AGG 0.615824 8.3:+65713166 MS.gene046374:CDS
AACTCCTGTTGGTCAACCGG+TGG 0.617457 8.3:+65712523 MS.gene046374:CDS
TTGCAATGCCGATGAAGCAG+CGG 0.626089 8.3:+65712474 MS.gene046374:CDS
GACGACTCTCCAAACAAACT+CGG 0.633316 8.3:+65714016 MS.gene046374:CDS
GAGCCTTGTCGCACAAGCGG+CGG 0.634283 8.3:-65711464 None:intergenic
GATTGTAAAATTGAATCAGA+CGG 0.636908 8.3:+65714678 MS.gene046374:CDS
TCTGGCGGCCTACAAACCAC+CGG 0.638956 8.3:-65712539 None:intergenic
TTCATCGGCATTGCAAGTGG+AGG 0.641324 8.3:-65712467 None:intergenic
GAAAGTGGCAATGATGAAAG+AGG 0.642013 8.3:-65712194 None:intergenic
CACACCAAATCCAAAACCAA+AGG 0.642886 8.3:+65713027 MS.gene046374:CDS
GACTTCGGAGTCCATTGTCG+CGG 0.643223 8.3:-65714605 None:intergenic
GGTTTGTTCCAACATTCATG+CGG 0.646426 8.3:-65714336 None:intergenic
TGAATGCATGCGCATCACCT+CGG 0.653083 8.3:-65714357 None:intergenic
AGATTATCGAATGCTTCTAG+TGG 0.659854 8.3:+65712035 MS.gene046374:CDS
GTTGACGTTAAAGGTGCAGA+CGG 0.660688 8.3:+65714403 MS.gene046374:CDS
GTGTTTCTCAGAGAAAACAA+TGG 0.661644 8.3:+65712363 MS.gene046374:CDS
CTTGGACTCAAACTCACCGG+TGG 0.662936 8.3:-65711722 None:intergenic
TGTAAAATTGAATCAGACGG+CGG 0.664291 8.3:+65714681 MS.gene046374:CDS
ATGAATGTTGGAACAAACCG+AGG 0.666462 8.3:+65714340 MS.gene046374:CDS
AGATGAACTATCATCATCCG+AGG 0.672198 8.3:-65715046 None:intergenic
AAGAACAAGACTGCACGTGA+CGG 0.684747 8.3:-65711856 None:intergenic
TCGATGTATGATGAAGGTAA+TGG 0.688963 8.3:+65711485 MS.gene046374:CDS
TCATTCTCCTCAAAACTAGG+TGG 0.698465 8.3:-65712632 None:intergenic
ATGTATGATGAAGGTAATGG+TGG 0.713594 8.3:+65711488 MS.gene046374:CDS
TCTTACACCTCCAAGTCACG+AGG 0.721324 8.3:+65713069 MS.gene046374:CDS
GGTGGTGCTAATGTTACAGG+TGG 0.742058 8.3:+65711506 MS.gene046374:CDS
GGAGGTGTAAGAACTGAACG+TGG 0.758096 8.3:-65713058 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGTTATTGTTACTATTTT+TGG + chr8.3:65712856-65712875 MS.gene046374:intron 15.0%
!!! TTGATTTAATTTAAGAAGTT+AGG + chr8.3:65713449-65713468 MS.gene046374:intron 15.0%
!!! TTTTAAAATCTAAAGTAGTT+TGG + chr8.3:65712939-65712958 MS.gene046374:intron 15.0%
!! AATGATAGAAACATTATTTG+TGG + chr8.3:65713000-65713019 MS.gene046374:CDS 20.0%
!! ACAATATAGCAATAAGATAA+TGG - chr8.3:65712797-65712816 None:intergenic 20.0%
!! GTTAATTAATCATAATCGTT+AGG + chr8.3:65713708-65713727 MS.gene046374:intron 20.0%
!!! AAGTTGGTAATATAAATGAA+AGG + chr8.3:65714929-65714948 MS.gene046374:intron 20.0%
!!! AATTTAGATGGGTTTTTTTT+TGG + chr8.3:65711962-65711981 MS.gene046374:intron 20.0%
!!! ACTTGAAAGTTTGTTAAAAA+TGG + chr8.3:65713952-65713971 MS.gene046374:CDS 20.0%
!!! ATTTAATTTAAGAAGTTAGG+TGG + chr8.3:65713452-65713471 MS.gene046374:intron 20.0%
! AAACATTTGTTTCATGTTTC+AGG + chr8.3:65714243-65714262 MS.gene046374:intron 25.0%
! AATCAATGAACAAGTTTATG+AGG + chr8.3:65714734-65714753 MS.gene046374:CDS 25.0%
! AATGGAAGAACCATAAATTA+TGG - chr8.3:65714214-65714233 None:intergenic 25.0%
! ACTTGATCAATATCTAAACA+AGG - chr8.3:65713869-65713888 None:intergenic 25.0%
! ATGGAAGAACCATAAATTAT+GGG - chr8.3:65714213-65714232 None:intergenic 25.0%
! CAAAGAAACAAAAACAAACA+AGG - chr8.3:65713301-65713320 None:intergenic 25.0%
! CATAAAAGTGAATCGTTATA+TGG + chr8.3:65713641-65713660 MS.gene046374:intron 25.0%
! CTTGATCAATATCTAAACAA+GGG - chr8.3:65713868-65713887 None:intergenic 25.0%
! GATTGTAAAATTGAATCAGA+CGG + chr8.3:65714678-65714697 MS.gene046374:CDS 25.0%
! TAAAAGAAACTTTACACTGA+TGG - chr8.3:65713684-65713703 None:intergenic 25.0%
! TTTAGTTAATGGTATGCAAA+TGG + chr8.3:65713360-65713379 MS.gene046374:intron 25.0%
!! AAATCTAAAGTAGTTTGGTT+TGG + chr8.3:65712944-65712963 MS.gene046374:intron 25.0%
!! AATTTCTTATTTAAGTGCTG+TGG + chr8.3:65713253-65713272 MS.gene046374:intron 25.0%
!! ATGTACCTTTTGCATTTAAA+AGG + chr8.3:65714071-65714090 MS.gene046374:CDS 25.0%
!! CTGATTCAATTTTACAATCT+CGG - chr8.3:65714677-65714696 None:intergenic 25.0%
!! TAACAAACTTTCAAGTAGTT+CGG - chr8.3:65713948-65713967 None:intergenic 25.0%
!!! ATAGCAATAAGATAATGGAA+TGG - chr8.3:65712792-65712811 None:intergenic 25.0%
!!! CTTGTTTGTTTTTGTTTCTT+TGG + chr8.3:65713299-65713318 MS.gene046374:intron 25.0%
!!! GCAATATTTTGAGACTTTTT+CGG - chr8.3:65713109-65713128 None:intergenic 25.0%
!!! TGTGTTCAAAAGTTTTTTTG+TGG + chr8.3:65713842-65713861 MS.gene046374:intron 25.0%
AAACTTGCAACAAAACATGA+GGG - chr8.3:65713208-65713227 None:intergenic 30.0%
AATGAACAAGTTTATGAGGA+TGG + chr8.3:65714738-65714757 MS.gene046374:CDS 30.0%
AATGATTGAATTGAAGCTAG+TGG + chr8.3:65713498-65713517 MS.gene046374:intron 30.0%
AATGGTAGTTGCACATTTAA+AGG + chr8.3:65713378-65713397 MS.gene046374:intron 30.0%
ACAAGGATTTACATAAGTGA+TGG - chr8.3:65713284-65713303 None:intergenic 30.0%
ACTACCATGAAGATTCTAAA+TGG - chr8.3:65714871-65714890 None:intergenic 30.0%
ACTTCTTAACAGTTCTTGAT+AGG + chr8.3:65714896-65714915 MS.gene046374:CDS 30.0%
AGGTAACAAATCTAATCCAA+AGG - chr8.3:65713328-65713347 None:intergenic 30.0%
ATCAGTAACACCTGTTAAAA+AGG - chr8.3:65713915-65713934 None:intergenic 30.0%
ATGAATCTTCATAGCAATCA+TGG + chr8.3:65712119-65712138 MS.gene046374:CDS 30.0%
CAAATGTTTCGATCAGAAAA+TGG - chr8.3:65714232-65714251 None:intergenic 30.0%
CAAGAAATCATTAGAACAGA+AGG + chr8.3:65714454-65714473 MS.gene046374:CDS 30.0%
CAATTTCTGATGTTTCAGAT+CGG + chr8.3:65712159-65712178 MS.gene046374:CDS 30.0%
CTTCTTAACAGTTCTTGATA+GGG + chr8.3:65714897-65714916 MS.gene046374:CDS 30.0%
CTTGCACATGATTTAGTTAA+TGG + chr8.3:65713349-65713368 MS.gene046374:intron 30.0%
GAAGTCTCTAAACTTTCAAA+AGG + chr8.3:65714637-65714656 MS.gene046374:CDS 30.0%
GAGTTATACCAACACTAATT+TGG + chr8.3:65713411-65713430 MS.gene046374:intron 30.0%
GGTTTGGATTGAATTGAATT+GGG + chr8.3:65712960-65712979 MS.gene046374:intron 30.0%
TAACTAAATCATGTGCAAGT+AGG - chr8.3:65713348-65713367 None:intergenic 30.0%
TAATTTAAGAAGTTAGGTGG+TGG + chr8.3:65713455-65713474 MS.gene046374:intron 30.0%
TAGTGATGTCCCATAATTTA+TGG + chr8.3:65714201-65714220 MS.gene046374:intron 30.0%
TGAATCTTCATAGCAATCAT+GGG + chr8.3:65712120-65712139 MS.gene046374:CDS 30.0%
TGATTCTGCAAATTTACGAA+CGG - chr8.3:65714719-65714738 None:intergenic 30.0%
TGTTTCTCAGAGAAAACAAT+GGG + chr8.3:65712364-65712383 MS.gene046374:CDS 30.0%
! AACCCATCTAAATTTTTCGT+CGG - chr8.3:65711956-65711975 None:intergenic 30.0%
! AATCAACTTGCCTTTTTAAC+AGG + chr8.3:65713902-65713921 MS.gene046374:intron 30.0%
! AATGATAGTGTAGTAGAATC+CGG + chr8.3:65714178-65714197 MS.gene046374:intron 30.0%
! TCATCCATTTAGAATCTTCA+TGG + chr8.3:65714864-65714883 MS.gene046374:CDS 30.0%
! TCTGAAACATCAGAAATTGT+TGG - chr8.3:65712158-65712177 None:intergenic 30.0%
! TGGTTTGGATTGAATTGAAT+TGG + chr8.3:65712959-65712978 MS.gene046374:intron 30.0%
! TTCTGCTGAGAAATTTTTGA+AGG + chr8.3:65714039-65714058 MS.gene046374:CDS 30.0%
!! ATTCAACAATTGGTGTTAAC+AGG - chr8.3:65712319-65712338 None:intergenic 30.0%
!! TTTGTTTCTTTGGAATCCTT+TGG + chr8.3:65713309-65713328 MS.gene046374:intron 30.0%
AAACCCTTACTTAAAAGAGC+TGG - chr8.3:65712747-65712766 None:intergenic 35.0%
AAAGAGACATTACAGTTCCT+CGG + chr8.3:65715029-65715048 MS.gene046374:CDS 35.0%
AAAGTTGAAGCCTTTGCATT+GGG + chr8.3:65712670-65712689 MS.gene046374:CDS 35.0%
AAATTATGGGACATCACTAC+CGG - chr8.3:65714200-65714219 None:intergenic 35.0%
AAGATCACAACCAAAATCAC+CGG + chr8.3:65711745-65711764 MS.gene046374:CDS 35.0%
AATGTTCTTGAAGCTTCTAG+AGG + chr8.3:65714294-65714313 MS.gene046374:CDS 35.0%
ACTCAATCTTGCAATGAAGA+TGG + chr8.3:65711999-65712018 MS.gene046374:intron 35.0%
ACTGGAGAAACCAATGTATT+TGG - chr8.3:65712605-65712624 None:intergenic 35.0%
AGAGGTTCTTTAAACGGAAA+TGG + chr8.3:65711560-65711579 MS.gene046374:CDS 35.0%
AGATTATCGAATGCTTCTAG+TGG + chr8.3:65712035-65712054 MS.gene046374:CDS 35.0%
AGATTCAACGGTGATAAAGA+CGG - chr8.3:65711929-65711948 None:intergenic 35.0%
ATTTCAGCTGAGAATTTGGT+GGG + chr8.3:65711611-65711630 MS.gene046374:CDS 35.0%
CACCGACGAAAAATTTAGAT+GGG + chr8.3:65711951-65711970 MS.gene046374:intron 35.0%
CAGAATTAAAACCTCCTTCA+AGG + chr8.3:65712558-65712577 MS.gene046374:CDS 35.0%
CCATTCTGATTCAAAACAGT+TGG - chr8.3:65712344-65712363 None:intergenic 35.0%
CTCCACTTCAGATTCAAAAT+TGG - chr8.3:65714119-65714138 None:intergenic 35.0%
GAAACTTGCAACAAAACATG+AGG - chr8.3:65713209-65713228 None:intergenic 35.0%
GAGGTTCTTTAAACGGAAAT+GGG + chr8.3:65711561-65711580 MS.gene046374:CDS 35.0%
GATGTTTGCCAAATTAGTGT+TGG - chr8.3:65713422-65713441 None:intergenic 35.0%
GTGTTTCTCAGAGAAAACAA+TGG + chr8.3:65712363-65712382 MS.gene046374:CDS 35.0%
TAAATCCTTTCAGACTCTAG+AGG + chr8.3:65714147-65714166 MS.gene046374:CDS 35.0%
TATTGCTATCTTACTTCGAG+CGG + chr8.3:65713123-65713142 MS.gene046374:CDS 35.0%
TCAAACTCCTCCAAATACAT+TGG + chr8.3:65712592-65712611 MS.gene046374:CDS 35.0%
TCACCGACGAAAAATTTAGA+TGG + chr8.3:65711950-65711969 MS.gene046374:intron 35.0%
TCGATGTATGATGAAGGTAA+TGG + chr8.3:65711485-65711504 MS.gene046374:CDS 35.0%
TCTATGAGCAGAACTAATCA+TGG - chr8.3:65714956-65714975 None:intergenic 35.0%
TCTCCAAGAGGTTCTTTAAA+CGG + chr8.3:65711554-65711573 MS.gene046374:CDS 35.0%
TCTTCATTCTCCTCAAAACT+AGG - chr8.3:65712638-65712657 None:intergenic 35.0%
TGTAAAATTGAATCAGACGG+CGG + chr8.3:65714681-65714700 MS.gene046374:CDS 35.0%
TTCTTTAAACGGAAATGGGT+CGG + chr8.3:65711565-65711584 MS.gene046374:CDS 35.0%
TTTCCGTTTAAAGAACCTCT+TGG - chr8.3:65711560-65711579 None:intergenic 35.0%
TTTGCATTGGGATAAAGTGA+GGG + chr8.3:65712682-65712701 MS.gene046374:CDS 35.0%
! AAATGGTTTGGGATCATTTG+AGG + chr8.3:65712720-65712739 MS.gene046374:CDS 35.0%
! ACAACATTTTCCGGTGATTT+TGG - chr8.3:65711758-65711777 None:intergenic 35.0%
! ACCTTTTGCATTTAAAAGGC+TGG + chr8.3:65714075-65714094 MS.gene046374:CDS 35.0%
! ACTCTACTAGAAGGTGTAAT+TGG - chr8.3:65712410-65712429 None:intergenic 35.0%
! AGCCAATTTTGAATCTGAAG+TGG + chr8.3:65714114-65714133 MS.gene046374:CDS 35.0%
! ATGTATGATGAAGGTAATGG+TGG + chr8.3:65711488-65711507 MS.gene046374:CDS 35.0%
! CATATGTACGACTTTAGAAG+TGG - chr8.3:65713763-65713782 None:intergenic 35.0%
! TCCAGCCTTTTAAATGCAAA+AGG - chr8.3:65714079-65714098 None:intergenic 35.0%
! TTTATGCTTTTAGTGACCAG+AGG - chr8.3:65713553-65713572 None:intergenic 35.0%
!! ACAGTTGGTGATTCAACAAT+TGG - chr8.3:65712329-65712348 None:intergenic 35.0%
!! CCAACTGTTTTGAATCAGAA+TGG + chr8.3:65712341-65712360 MS.gene046374:CDS 35.0%
!! CTAGAAGCATTCGATAATCT+CGG - chr8.3:65712035-65712054 None:intergenic 35.0%
!! GAGATCACAGAGTATTTTCA+TGG + chr8.3:65714820-65714839 MS.gene046374:CDS 35.0%
!! GTAATTGGTGTTGAAAGAAC+AGG - chr8.3:65712395-65712414 None:intergenic 35.0%
!! TTCATCATCGTCATTTTCTC+CGG - chr8.3:65712104-65712123 None:intergenic 35.0%
!!! CTTGAAGGAGGTTTTAATTC+TGG - chr8.3:65712560-65712579 None:intergenic 35.0%
!!! GAAGAGTTTTATTCTCCAAG+AGG + chr8.3:65711542-65711561 MS.gene046374:CDS 35.0%
!!! GATTTTGGTTGTGATCTTCT+TGG - chr8.3:65711743-65711762 None:intergenic 35.0%
!!! TAGTTTTGAGGAGAATGAAG+AGG + chr8.3:65712637-65712656 MS.gene046374:CDS 35.0%
ACCGGAAAATGTTGTAACTC+CGG + chr8.3:65711763-65711782 MS.gene046374:CDS 40.0%
ACTTCGAGCGGTTAATGTTA+CGG + chr8.3:65713135-65713154 MS.gene046374:CDS 40.0%
ATCATCGTCTTCTTCATCGT+CGG + chr8.3:65712061-65712080 MS.gene046374:CDS 40.0%
ATCCCAAACCATTTCACGAT+CGG - chr8.3:65712714-65712733 None:intergenic 40.0%
ATGAATGTTGGAACAAACCG+AGG + chr8.3:65714340-65714359 MS.gene046374:CDS 40.0%
CAAAGTTGAAGCCTTTGCAT+TGG + chr8.3:65712669-65712688 MS.gene046374:CDS 40.0%
CAAATTCTCAGCTGAAATCC+CGG - chr8.3:65711609-65711628 None:intergenic 40.0%
CAATGCAAAGGCTTCAACTT+TGG - chr8.3:65712671-65712690 None:intergenic 40.0%
CACACCAAATCCAAAACCAA+AGG + chr8.3:65713027-65713046 MS.gene046374:CDS 40.0%
CCAACTTCACTAACAAGTAG+TGG - chr8.3:65711800-65711819 None:intergenic 40.0%
CTCACTTTATCCCAATGCAA+AGG - chr8.3:65712683-65712702 None:intergenic 40.0%
CTTCTTCACATTCGCTAGAT+CGG - chr8.3:65714584-65714603 None:intergenic 40.0%
CTTTGCATTGGGATAAAGTG+AGG + chr8.3:65712681-65712700 MS.gene046374:CDS 40.0%
GAAAGTGGCAATGATGAAAG+AGG - chr8.3:65712197-65712216 None:intergenic 40.0%
GATAGGGTATGCAAAGAAGT+TGG + chr8.3:65714913-65714932 MS.gene046374:intron 40.0%
GATTGAATTGAATTGGGTGC+AGG + chr8.3:65712966-65712985 MS.gene046374:intron 40.0%
GATTTCAGCTGAGAATTTGG+TGG + chr8.3:65711610-65711629 MS.gene046374:CDS 40.0%
GGAGAAACCAATGTATTTGG+AGG - chr8.3:65712602-65712621 None:intergenic 40.0%
GGAGGAGTTTGAATCACAAA+AGG - chr8.3:65712584-65712603 None:intergenic 40.0%
GTAAAATTGAATCAGACGGC+GGG + chr8.3:65714682-65714701 MS.gene046374:CDS 40.0%
GTGCTCATTATATCTCTCTC+CGG - chr8.3:65712239-65712258 None:intergenic 40.0%
TCATTCTCCTCAAAACTAGG+TGG - chr8.3:65712635-65712654 None:intergenic 40.0%
TCTTCATAGCAATCATGGGT+TGG + chr8.3:65712124-65712143 MS.gene046374:CDS 40.0%
TCTTTAAACGGAAATGGGTC+GGG + chr8.3:65711566-65711585 MS.gene046374:CDS 40.0%
TGAATCACAAAAGGCCTTGA+AGG - chr8.3:65712575-65712594 None:intergenic 40.0%
TGTCTCTTTCCATGTAATCC+AGG - chr8.3:65715018-65715037 None:intergenic 40.0%
TTCATCTTCTTCTGCTTCAC+CGG + chr8.3:65711673-65711692 MS.gene046374:CDS 40.0%
TTCCGATCGTGAAATGGTTT+GGG + chr8.3:65712709-65712728 MS.gene046374:CDS 40.0%
TTCGTCGGTGAAAGATTCAA+CGG - chr8.3:65711941-65711960 None:intergenic 40.0%
TTTCCATGTAATCCAGGAAG+TGG - chr8.3:65715012-65715031 None:intergenic 40.0%
! AATGGTGGTGCTAATGTTAC+AGG + chr8.3:65711503-65711522 MS.gene046374:CDS 40.0%
! CCACTACTTGTTAGTGAAGT+TGG + chr8.3:65711797-65711816 MS.gene046374:CDS 40.0%
! GGTTTGTTCCAACATTCATG+CGG - chr8.3:65714339-65714358 None:intergenic 40.0%
!! ACAATTGGTGTTAACAGGTC+AGG - chr8.3:65712314-65712333 None:intergenic 40.0%
!! ACAGGTGTTACTGATACACT+TGG + chr8.3:65713920-65713939 MS.gene046374:intron 40.0%
!! AGGTCCAGCTCTTTTAAGTA+AGG + chr8.3:65712740-65712759 MS.gene046374:intron 40.0%
!! CTTTACCTCTAGAGTCTGAA+AGG - chr8.3:65714155-65714174 None:intergenic 40.0%
!! GCTGTTTTATAGCTATCCTC+TGG + chr8.3:65713534-65713553 MS.gene046374:intron 40.0%
!! GGTCCAGCTCTTTTAAGTAA+GGG + chr8.3:65712741-65712760 MS.gene046374:intron 40.0%
!!! GAAGGAGGTTTTAATTCTGG+CGG - chr8.3:65712557-65712576 None:intergenic 40.0%
!!! GCATCCTTTGGTTTTGGATT+TGG - chr8.3:65713034-65713053 None:intergenic 40.0%
AAACTAGGTGGCAACTCAAC+TGG - chr8.3:65712623-65712642 None:intergenic 45.0%
AACAACTCCTGTTGGTCAAC+CGG + chr8.3:65712520-65712539 MS.gene046374:CDS 45.0%
AACCATTTCACGATCGGAAC+TGG - chr8.3:65712708-65712727 None:intergenic 45.0%
AAGAACAAGACTGCACGTGA+CGG - chr8.3:65711859-65711878 None:intergenic 45.0%
AAGACCAAGTCTTCTGCACT+TGG - chr8.3:65714539-65714558 None:intergenic 45.0%
AAGTCACGAGGATAGGGTAT+TGG + chr8.3:65713081-65713100 MS.gene046374:CDS 45.0%
AATACCCTATCCTCGTGACT+TGG - chr8.3:65713082-65713101 None:intergenic 45.0%
AGACGGTGACTTACATTCTC+CGG - chr8.3:65711912-65711931 None:intergenic 45.0%
AGCGGTTAATGTTACGGTAG+AGG + chr8.3:65713141-65713160 MS.gene046374:CDS 45.0%
ATCACAAAAGGCCTTGAAGG+AGG - chr8.3:65712572-65712591 None:intergenic 45.0%
CAACCACTTCCTGGATTACA+TGG + chr8.3:65715006-65715025 MS.gene046374:CDS 45.0%
CAAGGCTCGATGTATGATGA+AGG + chr8.3:65711479-65711498 MS.gene046374:CDS 45.0%
CAAGTTTATGAGGATGGCTG+AGG + chr8.3:65714744-65714763 MS.gene046374:CDS 45.0%
CGATCTAGCGAATGTGAAGA+AGG + chr8.3:65714582-65714601 MS.gene046374:CDS 45.0%
CGGGATTTCAGCTGAGAATT+TGG + chr8.3:65711607-65711626 MS.gene046374:CDS 45.0%
CTAACAAGTAGTGGCTGTGT+CGG - chr8.3:65711791-65711810 None:intergenic 45.0%
CTCAAGCTGGTTGACGTTAA+AGG + chr8.3:65714394-65714413 MS.gene046374:CDS 45.0%
CTGATCGAATGAGAACGATC+CGG - chr8.3:65711695-65711714 None:intergenic 45.0%
CTTCTTGGACTCAAACTCAC+CGG - chr8.3:65711728-65711747 None:intergenic 45.0%
GACGACTCTCCAAACAAACT+CGG + chr8.3:65714016-65714035 MS.gene046374:CDS 45.0%
GCTTGATACACTTCTCAAGC+TGG + chr8.3:65714381-65714400 MS.gene046374:CDS 45.0%
GGAGCTGAAACTCTACTAGA+AGG - chr8.3:65712419-65712438 None:intergenic 45.0%
GTTAAAAATGGCGCCAAGCA+AGG + chr8.3:65713964-65713983 MS.gene046374:CDS 45.0%
GTTCCGATCGTGAAATGGTT+TGG + chr8.3:65712708-65712727 MS.gene046374:CDS 45.0%
TAGAGGCTGTGCTCAAAACT+GGG + chr8.3:65714311-65714330 MS.gene046374:CDS 45.0%
TCTCCAGAGAAAATCTTCGC+CGG + chr8.3:65711890-65711909 MS.gene046374:CDS 45.0%
TGTAATCCAGGAAGTGGTTG+TGG - chr8.3:65715006-65715025 None:intergenic 45.0%
TTCGATCAGTCTTTCTCCAC+CGG + chr8.3:65711706-65711725 MS.gene046374:CDS 45.0%
! AACCAGCTTCTAGGAAGCAT+TGG + chr8.3:65712282-65712301 MS.gene046374:CDS 45.0%
! AATTGGGTGCAGGTTGAATG+AGG + chr8.3:65712976-65712995 MS.gene046374:intron 45.0%
! GAAGTGGTTGTGGAAGCATA+GGG - chr8.3:65714996-65715015 None:intergenic 45.0%
! GCCGGAGTTACAACATTTTC+CGG - chr8.3:65711767-65711786 None:intergenic 45.0%
! GGAAGAAGAGCGCAAATTGA+AGG + chr8.3:65713985-65714004 MS.gene046374:CDS 45.0%
! GTTGACGTTAAAGGTGCAGA+CGG + chr8.3:65714403-65714422 MS.gene046374:CDS 45.0%
! TCAGCAGAACCGAGTTTGTT+TGG - chr8.3:65714028-65714047 None:intergenic 45.0%
!! ACCAGCTTCTAGGAAGCATT+GGG + chr8.3:65712283-65712302 MS.gene046374:CDS 45.0%
!! GAAAGAAGCGTTGGTGAAAG+TGG - chr8.3:65712212-65712231 None:intergenic 45.0%
!! GGAGTAGCATCCTTTGGTTT+TGG - chr8.3:65713040-65713059 None:intergenic 45.0%
!!! TGAGTTGCCACCTAGTTTTG+AGG + chr8.3:65712625-65712644 MS.gene046374:CDS 45.0%
ACCTCCAAGTCACGAGGATA+GGG + chr8.3:65713075-65713094 MS.gene046374:CDS 50.0%
ACTGGGAACCGCATGAATGT+TGG + chr8.3:65714328-65714347 MS.gene046374:CDS 50.0%
AGCATAGGGTTCACTGGAAC+AGG - chr8.3:65714982-65715001 None:intergenic 50.0%
ATGCTTCCACAACCACTTCC+TGG + chr8.3:65714997-65715016 MS.gene046374:CDS 50.0%
ATTGAATCAGACGGCGGGAT+CGG + chr8.3:65714687-65714706 MS.gene046374:CDS 50.0%
CATTGCTTCCGCTGCTTCAT+CGG - chr8.3:65712485-65712504 None:intergenic 50.0%
CCAACAGGAGTTGTTGGAGA+AGG - chr8.3:65712515-65712534 None:intergenic 50.0%
CCTTCTCCAACAACTCCTGT+TGG + chr8.3:65712512-65712531 MS.gene046374:CDS 50.0%
CTAGAGGCTGTGCTCAAAAC+TGG + chr8.3:65714310-65714329 MS.gene046374:CDS 50.0%
GAACGTGGAGTAGCATCCTT+TGG - chr8.3:65713046-65713065 None:intergenic 50.0%
GATGAAGCAGCGGAAGCAAT+GGG + chr8.3:65712484-65712503 MS.gene046374:CDS 50.0%
GATGCCAAGTGCAGAAGACT+TGG + chr8.3:65714532-65714551 MS.gene046374:CDS 50.0%
GGTTGACCAACAGGAGTTGT+TGG - chr8.3:65712521-65712540 None:intergenic 50.0%
GTTTAGAGACTTCGCTCGTG+AGG - chr8.3:65714630-65714649 None:intergenic 50.0%
GTTTATGAGGATGGCTGAGG+AGG + chr8.3:65714747-65714766 MS.gene046374:CDS 50.0%
TCCCAATGCTTCCTAGAAGC+TGG - chr8.3:65712287-65712306 None:intergenic 50.0%
TCGTCACTCAATGTTGCGCT+CGG - chr8.3:65714514-65714533 None:intergenic 50.0%
TCTTACACCTCCAAGTCACG+AGG + chr8.3:65713069-65713088 MS.gene046374:CDS 50.0%
TGAATGCATGCGCATCACCT+CGG - chr8.3:65714360-65714379 None:intergenic 50.0%
TGCTTCATCGGCATTGCAAG+TGG - chr8.3:65712473-65712492 None:intergenic 50.0%
TGCTTCCTAGAAGCTGGTTG+CGG - chr8.3:65712281-65712300 None:intergenic 50.0%
TTCATCGGCATTGCAAGTGG+AGG - chr8.3:65712470-65712489 None:intergenic 50.0%
TTGCAATGCCGATGAAGCAG+CGG + chr8.3:65712474-65712493 MS.gene046374:CDS 50.0%
TTGCGCTCTTCTTCCTTGCT+TGG - chr8.3:65713980-65713999 None:intergenic 50.0%
! GGAAGTGGTTGTGGAAGCAT+AGG - chr8.3:65714997-65715016 None:intergenic 50.0%
! GGAGGTGTAAGAACTGAACG+TGG - chr8.3:65713061-65713080 None:intergenic 50.0%
! GGTGGTGCTAATGTTACAGG+TGG + chr8.3:65711506-65711525 MS.gene046374:CDS 50.0%
! TCTCCGGCGAAGATTTTCTC+TGG - chr8.3:65711896-65711915 None:intergenic 50.0%
! TGTGGAAGCATAGGGTTCAC+TGG - chr8.3:65714988-65715007 None:intergenic 50.0%
! TTGGTCAACCGGTGGTTTGT+AGG + chr8.3:65712531-65712550 MS.gene046374:CDS 50.0%
!! ATCGTCATTTTCTCCGGCGA+TGG - chr8.3:65712098-65712117 None:intergenic 50.0%
!! GGAAGTGTGTGAAGCACTGT+TGG + chr8.3:65713162-65713181 MS.gene046374:CDS 50.0%
!! GTGTGTGAAGCACTGTTGGA+AGG + chr8.3:65713166-65713185 MS.gene046374:CDS 50.0%
AACTCCTGTTGGTCAACCGG+TGG + chr8.3:65712523-65712542 MS.gene046374:CDS 55.0%
AAGCTCGTTGTCGTCGTCAC+GGG + chr8.3:65711823-65711842 MS.gene046374:CDS 55.0%
ACCAACGCTTCTTTCGTCGC+CGG + chr8.3:65712217-65712236 MS.gene046374:CDS 55.0%
ACGGAAATGGGTCGGGTTCT+CGG + chr8.3:65711573-65711592 MS.gene046374:CDS 55.0%
ATCGGCATTGCAAGTGGAGG+TGG - chr8.3:65712467-65712486 None:intergenic 55.0%
CACCTCCAAGTCACGAGGAT+AGG + chr8.3:65713074-65713093 MS.gene046374:CDS 55.0%
CGATGAAGCAGCGGAAGCAA+TGG + chr8.3:65712483-65712502 MS.gene046374:CDS 55.0%
CTTGGACTCAAACTCACCGG+TGG - chr8.3:65711725-65711744 None:intergenic 55.0%
GAAGCTCGTTGTCGTCGTCA+CGG + chr8.3:65711822-65711841 MS.gene046374:CDS 55.0%
GACTTCGCTCGTGAGGACTT+CGG - chr8.3:65714623-65714642 None:intergenic 55.0%
GACTTCGGAGTCCATTGTCG+CGG - chr8.3:65714608-65714627 None:intergenic 55.0%
GGCCAGTTCCGATCGTGAAA+TGG + chr8.3:65712703-65712722 MS.gene046374:CDS 55.0%
GGCGTTTACTTTGCCATCGC+CGG + chr8.3:65712082-65712101 MS.gene046374:CDS 55.0%
TGTGAAGAAGGCCGCGACAA+TGG + chr8.3:65714594-65714613 MS.gene046374:CDS 55.0%
TTCCTAGAAGCTGGTTGCGG+CGG - chr8.3:65712278-65712297 None:intergenic 55.0%
! ACCCTATCCTCGTGACTTGG+AGG - chr8.3:65713079-65713098 None:intergenic 55.0%
! ATCGAGCCTTGTCGCACAAG+CGG - chr8.3:65711470-65711489 None:intergenic 55.0%
! ATTGCAAGTGGAGGTGGTGG+TGG - chr8.3:65712461-65712480 None:intergenic 55.0%
! CAAACCACCGGTTGACCAAC+AGG - chr8.3:65712530-65712549 None:intergenic 55.0%
! GGTTCTCGGAGAGTTTTCGC+CGG + chr8.3:65711587-65711606 MS.gene046374:CDS 55.0%
! GTTCTCGGAGAGTTTTCGCC+GGG + chr8.3:65711588-65711607 MS.gene046374:CDS 55.0%
GGCATTGCAAGTGGAGGTGG+TGG - chr8.3:65712464-65712483 None:intergenic 60.0%
TAGAAGCTGGTTGCGGCGGT+GGG - chr8.3:65712274-65712293 None:intergenic 60.0%
TCTGGCGGCCTACAAACCAC+CGG - chr8.3:65712542-65712561 None:intergenic 60.0%
TGTCGCACAAGCGGCGGTAA+CGG - chr8.3:65711461-65711480 None:intergenic 60.0%
TTACCGCCGCTTGTGCGACA+AGG + chr8.3:65711461-65711480 MS.gene046374:CDS 60.0%
! AGTAGTGGCTGTGTCGGTGC+CGG - chr8.3:65711785-65711804 None:intergenic 60.0%
! TCCGGCGACGAAAGAAGCGT+TGG - chr8.3:65712221-65712240 None:intergenic 60.0%
AGAAGCTGGTTGCGGCGGTG+GGG - chr8.3:65712273-65712292 None:intergenic 65.0%
CACCGCCGCAACCAGCTTCT+AGG + chr8.3:65712273-65712292 MS.gene046374:CDS 65.0%
CTAGAAGCTGGTTGCGGCGG+TGG - chr8.3:65712275-65712294 None:intergenic 65.0%
! GAGCCTTGTCGCACAAGCGG+CGG - chr8.3:65711467-65711486 None:intergenic 65.0%
! GGCGGTAACGGCTGTAGCTC+CGG - chr8.3:65711449-65711468 None:intergenic 65.0%
!! AGTGGAGGTGGTGGTGGTGG+TGG - chr8.3:65712455-65712474 None:intergenic 65.0%
!! GCAAGTGGAGGTGGTGGTGG+TGG - chr8.3:65712458-65712477 None:intergenic 65.0%
GAAGCTGGTTGCGGCGGTGG+GGG - chr8.3:65712272-65712291 None:intergenic 70.0%
!! GGAGGTGGTGGTGGTGGTGG+CGG - chr8.3:65712452-65712471 None:intergenic 70.0%
!! GGTGGTGGCGGTGGTGGTGG+AGG - chr8.3:65712440-65712459 None:intergenic 75.0%
!! GGTGGTGGTGGCGGTGGTGG+TGG - chr8.3:65712443-65712462 None:intergenic 75.0%
!! GGTGGTGGTGGTGGCGGTGG+TGG - chr8.3:65712446-65712465 None:intergenic 75.0%
!! GGTGGTGGTGGTGGTGGCGG+TGG - chr8.3:65712449-65712468 None:intergenic 75.0%
! GCTGGTTGCGGCGGTGGGGG+TGG - chr8.3:65712269-65712288 None:intergenic 80.0%
! GGTTGCGGCGGTGGGGGTGG+TGG - chr8.3:65712266-65712285 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 65711437 65715055 65711437 ID=MS.gene046374
chr8.3 mRNA 65711437 65715055 65711437 ID=MS.gene046374.t1;Parent=MS.gene046374
chr8.3 exon 65711437 65711917 65711437 ID=MS.gene046374.t1.exon1;Parent=MS.gene046374.t1
chr8.3 CDS 65711437 65711917 65711437 ID=cds.MS.gene046374.t1;Parent=MS.gene046374.t1
chr8.3 exon 65712018 65712756 65712018 ID=MS.gene046374.t1.exon2;Parent=MS.gene046374.t1
chr8.3 CDS 65712018 65712756 65712018 ID=cds.MS.gene046374.t1;Parent=MS.gene046374.t1
chr8.3 exon 65712988 65713187 65712988 ID=MS.gene046374.t1.exon3;Parent=MS.gene046374.t1
chr8.3 CDS 65712988 65713187 65712988 ID=cds.MS.gene046374.t1;Parent=MS.gene046374.t1
chr8.3 exon 65713924 65714168 65713924 ID=MS.gene046374.t1.exon4;Parent=MS.gene046374.t1
chr8.3 CDS 65713924 65714168 65713924 ID=cds.MS.gene046374.t1;Parent=MS.gene046374.t1
chr8.3 exon 65714265 65714918 65714265 ID=MS.gene046374.t1.exon5;Parent=MS.gene046374.t1
chr8.3 CDS 65714265 65714918 65714265 ID=cds.MS.gene046374.t1;Parent=MS.gene046374.t1
chr8.3 exon 65714975 65715055 65714975 ID=MS.gene046374.t1.exon6;Parent=MS.gene046374.t1
chr8.3 CDS 65714975 65715055 65714975 ID=cds.MS.gene046374.t1;Parent=MS.gene046374.t1
Gene Sequence

>MS.gene046374

ATGGACTCGCCGGAGCTACAGCCGTTACCGCCGCTTGTGCGACAAGGCTCGATGTATGATGAAGGTAATGGTGGTGCTAATGTTACAGGTGGTGAAGATGATGAAGAAGAGTTTTATTCTCCAAGAGGTTCTTTAAACGGAAATGGGTCGGGTTCTCGGAGAGTTTTCGCCGGGATTTCAGCTGAGAATTTGGTGGGTCGAAGTAGCAGTGAATCTACTTCTTCATCTTCTTCGTATTCATCTTCTTCTGCTTCACCGGATCGTTCTCATTCGATCAGTCTTTCTCCACCGGTGAGTTTGAGTCCAAGAAGATCACAACCAAAATCACCGGAAAATGTTGTAACTCCGGCACCGACACAGCCACTACTTGTTAGTGAAGTTGGAAGAAGCTCGTTGTCGTCGTCACGGGCTTCTTCTAACCGTCACGTGCAGTCTTGTTCTTCTATGTCTTCATCTCCAGAGAAAATCTTCGCCGGAGAATGTAAGTCACCGTCTTTATCACCGTTGAATCTTTCACCGACGAAAAATTTAGATGGGTTTTTTTTTGGTTAAAGTTGAAAAGACTCAATCTTGCAATGAAGATGGTTCTTCTTCGCCGAGATTATCGAATGCTTCTAGTGGTAAATCATCGTCTTCTTCATCGTCGGCGTTTACTTTGCCATCGCCGGAGAAAATGACGATGATGAATCTTCATAGCAATCATGGGTTGGATCAGTCTCCAACAATTTCTGATGTTTCAGATCGGTTTAGACATTCTCCTCTTTCATCATTGCCACTTTCACCAACGCTTCTTTCGTCGCCGGAGAGAGATATAATGAGCACTCAACCACCACCCCCACCGCCGCAACCAGCTTCTAGGAAGCATTGGGAGATTCCTGACCTGTTAACACCAATTGTTGAATCACCAACTGTTTTGAATCAGAATGGTGTTTCTCAGAGAAAACAATGGGAAATTCCTGTTCTTTCAACACCAATTACACCTTCTAGTAGAGTTTCAGCTCCTCCACCACCACCGCCACCACCACCACCACCTCCACTTGCAATGCCGATGAAGCAGCGGAAGCAATGGGAAGTACCTTCTCCAACAACTCCTGTTGGTCAACCGGTGGTTTGTAGGCCGCCAGAATTAAAACCTCCTTCAAGGCCTTTTGTGATTCAAACTCCTCCAAATACATTGGTTTCTCCAGTTGAGTTGCCACCTAGTTTTGAGGAGAATGAAGAGGTTTCTAAACCAAAGTTGAAGCCTTTGCATTGGGATAAAGTGAGGGCCAGTTCCGATCGTGAAATGGTTTGGGATCATTTGAGGTCCAGCTCTTTTAAGTAAGGGTTTCAATTCAACTCAACTATCATCACCATTCCATTATCTTATTGCTATATTGTTGATTCTTGCTTTTTATAGATTATTATAGATTATTGATAATTGTTATTGTTACTATTTTTGGAGTGTTAGTTATAAGTAGTTGATTATTCTGATGTTTTTTTCGTACTATATTGCAATATGTTTTTAAAATCTAAAGTAGTTTGGTTTGGATTGAATTGAATTGGGTGCAGGTTGAATGAGGAAATGATAGAAACATTATTTGTGGTGAACACACCAAATCCAAAACCAAAGGATGCTACTCCACGTTCAGTTCTTACACCTCCAAGTCACGAGGATAGGGTATTGGATCCGAAAAAGTCTCAAAATATTGCTATCTTACTTCGAGCGGTTAATGTTACGGTAGAGGAAGTGTGTGAAGCACTGTTGGAAGGTATGTATTTTATTTTTCCCTCATGTTTTGTTGCAAGTTTCTATTGATTAGATCTGTTGTTGAAAAATTTCTTATTTAAGTGCTGTGGTTAAGCCATCACTTATGTAAATCCTTGTTTGTTTTTGTTTCTTTGGAATCCTTTGGATTAGATTTGTTACCTACTTGCACATGATTTAGTTAATGGTATGCAAATGGTAGTTGCACATTTAAAGGAAAAACAATTGAGTTATACCAACACTAATTTGGCAAACATCTTTTGCTTTGATTTAATTTAAGAAGTTAGGTGGTGGTATTATTATTACATATATCAAATGATTGAATTGAAGCTAGTGGACAGTTTTGTTATGCTGTTTTATAGCTATCCTCTGGTCACTAAAAGCATAAATGTGTGCTTCATAAATCAATGCTTTCGTTGTCTCTCTTTTACGATGTTCTCTCTCAATTTTATCTTTACATAAAAGTGAATCGTTATATGGATTTAAATGTATATACACCATCAGTGTAAAGTTTCTTTTACATTGTTAATTAATCATAATCGTTAGGTCATTAAGAACATTTGACTTCAATTATAGCCACTTCTAAAGTCGTACATATGACTGATTGTGATTTCTTGACATTGCAAAACTTTTTACAAAAATTGAACTCTTGTTATAATGTGTTCAAAAGTTTTTTTGTGGCCCTTGTTTAGATATTGATCAAGTAAATAAGATGATAAATCAACTTGCCTTTTTAACAGGTGTTACTGATACACTTGGAACCGAACTACTTGAAAGTTTGTTAAAAATGGCGCCAAGCAAGGAAGAAGAGCGCAAATTGAAGGAACATAAAGACGACTCTCCAAACAAACTCGGTTCTGCTGAGAAATTTTTGAAGGCAGTTCTCGATGTACCTTTTGCATTTAAAAGGCTGGAAGCTATGCTTTACATAGCCAATTTTGAATCTGAAGTGGAGTATATTCGTAAATCCTTTCAGACTCTAGAGGTAAAGTGTAATGATAGTGTAGTAGAATCCGGTAGTGATGTCCCATAATTTATGGTTCTTCCATTTTCTGATCGAAACATTTGTTTCATGTTTCAGGTTGCATGTGAAGAGCTGCGAAACAGTAGAATGTTCTTGAAGCTTCTAGAGGCTGTGCTCAAAACTGGGAACCGCATGAATGTTGGAACAAACCGAGGTGATGCGCATGCATTCAAGCTTGATACACTTCTCAAGCTGGTTGACGTTAAAGGTGCAGACGGTAAAACAACTCTTCTACACTTCGTTGTACAAGAAATCATTAGAACAGAAGGAGCTCGTCATTCAGATACTTCTACTAATCAAACACCGAGCGCAACATTGAGTGACGATGCCAAGTGCAGAAGACTTGGTCTTCAAGTAGTATCTAGTCTAAGTTCCGATCTAGCGAATGTGAAGAAGGCCGCGACAATGGACTCCGAAGTCCTCACGAGCGAAGTCTCTAAACTTTCAAAAGGAATCACACACATCGCCGAGATTGTAAAATTGAATCAGACGGCGGGATCGGATGAAACCGTTCGTAAATTTGCAGAATCAATGAACAAGTTTATGAGGATGGCTGAGGAGGAGATTTTAAGAATTCAAGCACAAGAAAGTGTTGCTTTATCACTTGTGAAAGAGATCACAGAGTATTTTCATGGTAACTTATCTAAAGAAGAAGCTCATCCATTTAGAATCTTCATGGTAGTAAGAGACTTCTTAACAGTTCTTGATAGGGTATGCAAAGAAGTTGGTAATATAAATGAAAGGACCATGATTAGTTCTGCTCATAGATTTCCTGTTCCAGTGAACCCTATGCTTCCACAACCACTTCCTGGATTACATGGAAAGAGACATTACAGTTCCTCGGATGA
Protein sequence

>MS.gene046374.t1

MDSPELQPLPPLVRQGSMYDEGNGGANVTGGEDDEEEFYSPRGSLNGNGSGSRRVFAGISAENLVGRSSSESTSSSSSYSSSSASPDRSHSISLSPPVSLSPRRSQPKSPENVVTPAPTQPLLVSEVGRSSLSSSRASSNRHVQSCSSMSSSPEKIFAGEYGSSSPRLSNASSGKSSSSSSSAFTLPSPEKMTMMNLHSNHGLDQSPTISDVSDRFRHSPLSSLPLSPTLLSSPERDIMSTQPPPPPPQPASRKHWEIPDLLTPIVESPTVLNQNGVSQRKQWEIPVLSTPITPSSRVSAPPPPPPPPPPPPLAMPMKQRKQWEVPSPTTPVGQPVVCRPPELKPPSRPFVIQTPPNTLVSPVELPPSFEENEEVSKPKLKPLHWDKVRASSDREMVWDHLRSSSFKLNEEMIETLFVVNTPNPKPKDATPRSVLTPPSHEDRVLDPKKSQNIAILLRAVNVTVEEVCEALLEGVTDTLGTELLESLLKMAPSKEEERKLKEHKDDSPNKLGSAEKFLKAVLDVPFAFKRLEAMLYIANFESEVEYIRKSFQTLEVACEELRNSRMFLKLLEAVLKTGNRMNVGTNRGDAHAFKLDTLLKLVDVKGADGKTTLLHFVVQEIIRTEGARHSDTSTNQTPSATLSDDAKCRRLGLQVVSSLSSDLANVKKAATMDSEVLTSEVSKLSKGITHIAEIVKLNQTAGSDETVRKFAESMNKFMRMAEEEILRIQAQESVALSLVKEITEYFHGNLSKEEAHPFRIFMVVRDFLTVLDRISCSSEPYASTTTSWITWKETLQFLG