Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04782.t1 | XP_003630836.1 | 99.4 | 683 | 2 | 1 | 1 | 681 | 1 | 683 | 0.00E+00 | 1147.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04782.t1 | Q9LS42 | 74.1 | 691 | 167 | 3 | 1 | 681 | 1 | 689 | 9.2e-252 | 870.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04782.t1 | G7L912 | 99.4 | 683 | 2 | 1 | 1 | 681 | 1 | 683 | 0.0e+00 | 1147.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene04782 | MS.gene82345 | PPI |
| MS.gene04782 | MS.gene56498 | PPI |
| MS.gene04782 | MS.gene006825 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04782.t1 | MTR_8g103950 | 99.414 | 683 | 2 | 1 | 1 | 681 | 1 | 683 | 0.0 | 1369 |
| MS.gene04782.t1 | MTR_8g103950 | 99.476 | 572 | 1 | 1 | 1 | 570 | 1 | 572 | 0.0 | 1131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04782.t1 | AT3G18480 | 79.450 | 691 | 130 | 3 | 1 | 681 | 1 | 689 | 0.0 | 1099 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCCGTCGTCTCCGCTTTC+TGG | 0.108455 | 4.2:+1945219 | MS.gene04782:CDS |
| GAAAGTTCCTTTATGATCTT+TGG | 0.215035 | 4.2:-1951834 | None:intergenic |
| TTCACATTTAACAGGAAATA+AGG | 0.255632 | 4.2:+1952479 | MS.gene04782:intron |
| CGAGCACAAACAATGCTTCT+TGG | 0.272686 | 4.2:+1948097 | MS.gene04782:CDS |
| AGGCAGTTGAAGGAGAAAAT+TGG | 0.293467 | 4.2:+1952499 | MS.gene04782:CDS |
| TTCAGAGATTAACTTCTCTT+TGG | 0.299122 | 4.2:-1949888 | None:intergenic |
| TGCCTTGCTTAAGGGGTATC+AGG | 0.303133 | 4.2:+1946120 | MS.gene04782:CDS |
| GATACAAGGAGTTGGGATTC+AGG | 0.303649 | 4.2:+1953480 | MS.gene04782:CDS |
| GGCAGTTGAAGGAGAAAATT+GGG | 0.322531 | 4.2:+1952500 | MS.gene04782:CDS |
| TGTCAGCTTTGAGTTATATA+AGG | 0.327795 | 4.2:+1953808 | MS.gene04782:CDS |
| TGAGCTTTGATCTTGATCTA+AGG | 0.357904 | 4.2:-1952279 | None:intergenic |
| TTGTTTAATGCCTTGCTTAA+GGG | 0.370199 | 4.2:+1946112 | MS.gene04782:CDS |
| ACAAACAATGCTTCTTGGTT+TGG | 0.373165 | 4.2:+1948102 | MS.gene04782:CDS |
| TTCTTCACGTTCATTTGATA+AGG | 0.377695 | 4.2:-1949942 | None:intergenic |
| AACTTCATCCATCAGGAGAT+TGG | 0.381423 | 4.2:-1948071 | None:intergenic |
| TCCTTTATGATCTTTGGAAC+TGG | 0.384238 | 4.2:-1951828 | None:intergenic |
| TTTGTTTAATGCCTTGCTTA+AGG | 0.387765 | 4.2:+1946111 | MS.gene04782:CDS |
| TATAAAAGAATGCAAAAGTT+CGG | 0.398780 | 4.2:-1953743 | None:intergenic |
| GGCAATGAAGAAAGAGCTTC+AGG | 0.400714 | 4.2:+1950996 | MS.gene04782:CDS |
| CACTGCAATGCTTAATGAAA+AGG | 0.405025 | 4.2:+1949988 | MS.gene04782:CDS |
| AAGTAGGCAATTAGAGCAAC+AGG | 0.405707 | 4.2:+1947375 | MS.gene04782:CDS |
| GCAGTCAGAGAGATCGATTT+AGG | 0.415746 | 4.2:+1952318 | MS.gene04782:CDS |
| ATAAAAGAATGCAAAAGTTC+GGG | 0.416558 | 4.2:-1953742 | None:intergenic |
| TACAATCGGGTTGCATGTCT+TGG | 0.416848 | 4.2:+1953764 | MS.gene04782:CDS |
| CAACACCTTCTTAGAACCCC+TGG | 0.427405 | 4.2:-1952620 | None:intergenic |
| GGGATCTATCAGAGGCTAAT+CGG | 0.429590 | 4.2:+1951867 | MS.gene04782:CDS |
| CTCTTCAAGTCTTCGTATTG+TGG | 0.446914 | 4.2:-1947350 | None:intergenic |
| CTCGTTCAACTTCATCCATC+AGG | 0.450947 | 4.2:-1948078 | None:intergenic |
| AGAGAAAGTAAAAGAAATTG+TGG | 0.451660 | 4.2:+1947521 | MS.gene04782:CDS |
| AAGGAACTTTCCTTGATGAC+TGG | 0.453666 | 4.2:+1951846 | MS.gene04782:CDS |
| TCCAGTTCCAAAGATCATAA+AGG | 0.457304 | 4.2:+1951827 | MS.gene04782:CDS |
| ATTCAATTGAGGCTGAAGAC+TGG | 0.460183 | 4.2:+1951190 | MS.gene04782:CDS |
| TTTCTTTAGCACTCTGCAGC+TGG | 0.464259 | 4.2:-1947770 | None:intergenic |
| AAAGCGGAGACGACGGAGAT+CGG | 0.467188 | 4.2:-1945215 | None:intergenic |
| GCAGATAATGTTAAACTGTA+CGG | 0.472098 | 4.2:+1952553 | MS.gene04782:CDS |
| GCAAGAGCTGGATAAGGATC+TGG | 0.474947 | 4.2:-1946223 | None:intergenic |
| ACAGGAAATAAGGCAGTTGA+AGG | 0.475055 | 4.2:+1952489 | MS.gene04782:intron |
| TTTGAACTCCGTGCTCAGTC+TGG | 0.477470 | 4.2:+1947840 | MS.gene04782:CDS |
| ATACAAGGAGTTGGGATTCA+GGG | 0.479433 | 4.2:+1953481 | MS.gene04782:CDS |
| GTCGTCTCCGCTTTCTGGAA+AGG | 0.483279 | 4.2:+1945224 | MS.gene04782:CDS |
| GATCAAAGATACAAGGAGTT+GGG | 0.483830 | 4.2:+1953473 | MS.gene04782:CDS |
| GAGCACGGAGTTCAAACAAC+TGG | 0.486238 | 4.2:-1947833 | None:intergenic |
| GGTTTCCAGGGGTTCTAAGA+AGG | 0.490914 | 4.2:+1952615 | MS.gene04782:CDS |
| AACTCACTTGAAGAATCAAC+AGG | 0.495755 | 4.2:+1947327 | MS.gene04782:CDS |
| TGTTTAATGCCTTGCTTAAG+GGG | 0.498851 | 4.2:+1946113 | MS.gene04782:CDS |
| AGATCAAAGATACAAGGAGT+TGG | 0.502305 | 4.2:+1953472 | MS.gene04782:CDS |
| CAGATAATGTTAAACTGTAC+GGG | 0.503919 | 4.2:+1952554 | MS.gene04782:CDS |
| AAACAAACTTGACTTATCCT+CGG | 0.505879 | 4.2:-1946095 | None:intergenic |
| CCGAGCTAGAATCTGAGAAC+CGG | 0.509206 | 4.2:+1947266 | MS.gene04782:CDS |
| ACTGAGGCAAGAGCTGGATA+AGG | 0.510141 | 4.2:-1946229 | None:intergenic |
| CTGATAGATCCCAGTCATCA+AGG | 0.512241 | 4.2:-1951856 | None:intergenic |
| AGCAGCGGACGATTTCTTCT+CGG | 0.512425 | 4.2:+1953518 | MS.gene04782:CDS |
| AACAAACTTGACTTATCCTC+GGG | 0.514878 | 4.2:-1946094 | None:intergenic |
| GCTCATCTCCTCCCCACTTG+TGG | 0.514899 | 4.2:-1951220 | None:intergenic |
| TTCAATTGAGGCTGAAGACT+GGG | 0.516424 | 4.2:+1951191 | MS.gene04782:CDS |
| TCCCTCTCCGATCCACTTGG+CGG | 0.520914 | 4.2:-1945179 | None:intergenic |
| CCGGTTCTCAGATTCTAGCT+CGG | 0.521831 | 4.2:-1947266 | None:intergenic |
| AGCTGACATTCTGTAGAGAC+AGG | 0.526667 | 4.2:-1953793 | None:intergenic |
| ACACGAGCTAACACAGTTGA+AGG | 0.534521 | 4.2:+1951284 | MS.gene04782:CDS |
| AGGAACTTTCCTTGATGACT+GGG | 0.534962 | 4.2:+1951847 | MS.gene04782:CDS |
| GCTTAAGGGGTATCAGGAAG+AGG | 0.536160 | 4.2:+1946126 | MS.gene04782:CDS |
| GGCTCAGATGAAACACTGGC+TGG | 0.536356 | 4.2:+1953913 | MS.gene04782:CDS |
| GATATAGGAGGCTTCTATTG+TGG | 0.539708 | 4.2:+1950975 | MS.gene04782:intron |
| TTGCAGGGAACAATTACTAC+AGG | 0.540685 | 4.2:+1947746 | MS.gene04782:intron |
| GACTGGGAAGTGGCCACAAG+TGG | 0.542533 | 4.2:+1951207 | MS.gene04782:CDS |
| GTGTCCCTCTCCGATCCACT+TGG | 0.542837 | 4.2:-1945182 | None:intergenic |
| CAGTCCACGTGGAAGATCAA+TGG | 0.546836 | 4.2:-1953944 | None:intergenic |
| GGTGGCAGAACTTTCAGCTA+AGG | 0.546982 | 4.2:+1951626 | MS.gene04782:CDS |
| GAAGACTTGAAGAGCGAAGT+AGG | 0.548247 | 4.2:+1947359 | MS.gene04782:CDS |
| ACAGCAACTGAGGCAAGAGC+TGG | 0.549391 | 4.2:-1946235 | None:intergenic |
| GGAGTCTCCGCCAAGTGGAT+CGG | 0.552009 | 4.2:+1945172 | MS.gene04782:CDS |
| TATGCAGAAGATCTTGAAAG+TGG | 0.552427 | 4.2:+1952738 | MS.gene04782:CDS |
| AGTGGGTTATAATTCAATTG+AGG | 0.558132 | 4.2:+1951179 | MS.gene04782:CDS |
| ATCTTCCACGTGGACTGTAG+TGG | 0.562365 | 4.2:+1953950 | MS.gene04782:CDS |
| ATCATCAGAATATGGATCAA+AGG | 0.565231 | 4.2:+1952252 | MS.gene04782:intron |
| GTTGGAAGATGATATATCCA+AGG | 0.569133 | 4.2:+1951677 | MS.gene04782:CDS |
| AATCCGACCTTTCCAGAAAG+CGG | 0.571885 | 4.2:-1945231 | None:intergenic |
| TCCGCCAAGTGGATCGGAGA+GGG | 0.574061 | 4.2:+1945178 | MS.gene04782:CDS |
| GACGACGGAGATCGGAGAGT+TGG | 0.574510 | 4.2:-1945207 | None:intergenic |
| ACTCGAAACAGCCGAGCAGA+AGG | 0.575671 | 4.2:+1951605 | MS.gene04782:CDS |
| GGAAGTGGCCACAAGTGGGG+AGG | 0.576452 | 4.2:+1951212 | MS.gene04782:CDS |
| TCTTGGTTTGGAGAGAGAGA+AGG | 0.577076 | 4.2:+1948114 | MS.gene04782:CDS |
| TGAGGCTGAAGACTGGGAAG+TGG | 0.578334 | 4.2:+1951197 | MS.gene04782:CDS |
| CTGGGAAGTGGCCACAAGTG+GGG | 0.580493 | 4.2:+1951209 | MS.gene04782:CDS |
| TTTGATTACCAGACTGAGCA+CGG | 0.583328 | 4.2:-1947848 | None:intergenic |
| TGGGGAGGAGATGAGCAAGA+TGG | 0.591522 | 4.2:+1951227 | MS.gene04782:CDS |
| GGAAAGAGATCAAAGATACA+AGG | 0.597550 | 4.2:+1953466 | MS.gene04782:intron |
| GAAAATGAAAGTTGAGCTTG+AGG | 0.599598 | 4.2:+1947288 | MS.gene04782:CDS |
| AAGATTCTGAAACTAAGAAG+AGG | 0.601242 | 4.2:+1948813 | MS.gene04782:CDS |
| CGAAACAGCCGAGCAGAAGG+TGG | 0.605027 | 4.2:+1951608 | MS.gene04782:CDS |
| TGGTCATACATTCAGGCAGT+GGG | 0.605843 | 4.2:+1951162 | MS.gene04782:intron |
| AGCAGAGGCCAATCTCCTGA+TGG | 0.608030 | 4.2:+1948063 | MS.gene04782:CDS |
| AAGTTCTGCCACCTTCTGCT+CGG | 0.610438 | 4.2:-1951616 | None:intergenic |
| GAGGGCAGCTAAGCAAGCAG+AGG | 0.612345 | 4.2:+1948048 | MS.gene04782:CDS |
| TTTCCAGAAAGCGGAGACGA+CGG | 0.616138 | 4.2:-1945222 | None:intergenic |
| AAATCTGTGTTAGATGAACA+AGG | 0.616404 | 4.2:+1945905 | MS.gene04782:CDS |
| TAATCGGAGTGAGGTGTCTG+AGG | 0.620394 | 4.2:+1951883 | MS.gene04782:CDS |
| TCATGGCTCAGATGAAACAC+TGG | 0.624075 | 4.2:+1953909 | MS.gene04782:CDS |
| GCTTGAGGAATTTAGAACAG+AGG | 0.635432 | 4.2:+1947303 | MS.gene04782:CDS |
| GTTAATCTCTGAATTGAACA+TGG | 0.637889 | 4.2:+1949898 | MS.gene04782:CDS |
| ACTGGGAAGTGGCCACAAGT+GGG | 0.638410 | 4.2:+1951208 | MS.gene04782:CDS |
| GACAGAATCACACTCAGCAG+CGG | 0.644787 | 4.2:+1953503 | MS.gene04782:CDS |
| TGATGACTGGGATCTATCAG+AGG | 0.646009 | 4.2:+1951859 | MS.gene04782:CDS |
| TTCCTGATACCCCTTAAGCA+AGG | 0.649337 | 4.2:-1946122 | None:intergenic |
| AAAATGGAGTCTCCGCCAAG+TGG | 0.659317 | 4.2:+1945167 | None:intergenic |
| ACGCTTGAGAGAGACAGAAG+AGG | 0.662085 | 4.2:+1952343 | MS.gene04782:CDS |
| ATCAGAGGCTAATCGGAGTG+AGG | 0.670414 | 4.2:+1951874 | MS.gene04782:CDS |
| GCTGGAACTCACAGCAACTG+AGG | 0.677059 | 4.2:-1946245 | None:intergenic |
| TAAGCATCGCAATCTAGCAG+AGG | 0.677217 | 4.2:+1947548 | MS.gene04782:CDS |
| TTTCAAGAAAGCTTCACCCG+AGG | 0.686730 | 4.2:+1946078 | MS.gene04782:CDS |
| AATCTGTGTTAGATGAACAA+GGG | 0.690995 | 4.2:+1945906 | MS.gene04782:CDS |
| GCAGTTGAAGGAGAAAATTG+GGG | 0.693185 | 4.2:+1952501 | MS.gene04782:CDS |
| CTCCGCCAAGTGGATCGGAG+AGG | 0.701318 | 4.2:+1945177 | MS.gene04782:CDS |
| GAAAATTGGGGTATTAACAG+CGG | 0.709114 | 4.2:+1952513 | MS.gene04782:CDS |
| AGAACCATTGATCTTCCACG+TGG | 0.765127 | 4.2:+1953940 | MS.gene04782:CDS |
| AAAATACAAGAAGATATACG+AGG | 0.768531 | 4.2:+1952782 | MS.gene04782:CDS |
| TATACCCACTACAGTCCACG+TGG | 0.774860 | 4.2:-1953955 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 1945170 | 1953969 | 1945170 | ID=MS.gene04782 |
| chr4.2 | mRNA | 1945170 | 1953969 | 1945170 | ID=MS.gene04782.t1;Parent=MS.gene04782 |
| chr4.2 | exon | 1945170 | 1945245 | 1945170 | ID=MS.gene04782.t1.exon1;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1945170 | 1945245 | 1945170 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1945885 | 1945995 | 1945885 | ID=MS.gene04782.t1.exon2;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1945885 | 1945995 | 1945885 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1946077 | 1946255 | 1946077 | ID=MS.gene04782.t1.exon3;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1946077 | 1946255 | 1946077 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1947247 | 1947396 | 1947247 | ID=MS.gene04782.t1.exon4;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1947247 | 1947396 | 1947247 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1947516 | 1947604 | 1947516 | ID=MS.gene04782.t1.exon5;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1947516 | 1947604 | 1947516 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1947752 | 1947861 | 1947752 | ID=MS.gene04782.t1.exon6;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1947752 | 1947861 | 1947752 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1948041 | 1948135 | 1948041 | ID=MS.gene04782.t1.exon7;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1948041 | 1948135 | 1948041 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1948779 | 1948834 | 1948779 | ID=MS.gene04782.t1.exon8;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1948779 | 1948834 | 1948779 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1949844 | 1950009 | 1949844 | ID=MS.gene04782.t1.exon9;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1949844 | 1950009 | 1949844 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1950982 | 1951074 | 1950982 | ID=MS.gene04782.t1.exon10;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1950982 | 1951074 | 1950982 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1951177 | 1951305 | 1951177 | ID=MS.gene04782.t1.exon11;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1951177 | 1951305 | 1951177 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1951579 | 1951698 | 1951579 | ID=MS.gene04782.t1.exon12;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1951579 | 1951698 | 1951579 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1951818 | 1951904 | 1951818 | ID=MS.gene04782.t1.exon13;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1951818 | 1951904 | 1951818 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1952260 | 1952364 | 1952260 | ID=MS.gene04782.t1.exon14;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1952260 | 1952364 | 1952260 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1952493 | 1952636 | 1952493 | ID=MS.gene04782.t1.exon15;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1952493 | 1952636 | 1952493 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1952738 | 1952839 | 1952738 | ID=MS.gene04782.t1.exon16;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1952738 | 1952839 | 1952738 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1953467 | 1953546 | 1953467 | ID=MS.gene04782.t1.exon17;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1953467 | 1953546 | 1953467 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1953737 | 1953829 | 1953737 | ID=MS.gene04782.t1.exon18;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1953737 | 1953829 | 1953737 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| chr4.2 | exon | 1953909 | 1953969 | 1953909 | ID=MS.gene04782.t1.exon19;Parent=MS.gene04782.t1 |
| chr4.2 | CDS | 1953909 | 1953969 | 1953909 | ID=cds.MS.gene04782.t1;Parent=MS.gene04782.t1 |
| Gene Sequence |
| Protein sequence |
