AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04945


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04945.t1 MTR_5g027890 55.939 261 112 2 1 261 1 258 1.28e-93 278
MS.gene04945.t1 MTR_6g015890 56.589 258 101 4 8 258 7 260 3.21e-92 276
MS.gene04945.t1 MTR_6g015900 54.275 269 111 5 8 269 4 267 3.66e-89 267
MS.gene04945.t1 MTR_3g080330 53.008 266 117 4 10 273 2 261 6.22e-88 263
MS.gene04945.t1 MTR_3g067615 55.720 271 106 5 19 287 3 261 1.12e-87 263
MS.gene04945.t1 MTR_3g067630 55.061 247 106 2 8 253 8 250 9.60e-86 260
MS.gene04945.t1 MTR_3g067625 54.651 258 112 2 1 257 1 254 2.19e-83 259
MS.gene04945.t1 MTR_3g067625 56.098 82 23 2 8 87 382 452 3.41e-18 84.7
MS.gene04945.t1 MTR_5g036740 48.736 277 123 3 10 273 2 272 6.19e-81 246
MS.gene04945.t1 MTR_3g065620 50.903 277 122 6 1 276 1 264 1.02e-79 243
MS.gene04945.t1 MTR_8g091730 49.084 273 118 5 1 259 2 267 3.88e-79 242
MS.gene04945.t1 MTR_1g045100 47.902 286 133 8 2 274 3 285 6.18e-76 233
MS.gene04945.t1 MTR_3g080310 50.973 257 110 4 10 259 2 249 8.35e-76 232
MS.gene04945.t1 MTR_3g080280 51.724 261 119 5 7 267 12 265 1.04e-75 233
MS.gene04945.t1 MTR_3g067645 52.206 272 120 6 1 270 2 265 3.29e-73 228
MS.gene04945.t1 MTR_1g044795 49.442 269 124 5 1 259 1 267 7.44e-73 226
MS.gene04945.t1 MTR_3g065820 49.430 263 127 4 1 259 1 261 1.26e-69 218
MS.gene04945.t1 MTR_2g018910 47.674 258 127 5 20 273 27 280 1.35e-67 214
MS.gene04945.t1 MTR_3g080360 44.867 263 131 4 1 258 21 274 1.70e-66 209
MS.gene04945.t1 MTR_3g067990 46.008 263 106 6 3 253 2 240 2.25e-65 206
MS.gene04945.t1 MTR_2g018950 44.484 281 133 6 20 288 23 292 8.95e-64 204
MS.gene04945.t1 MTR_3g065590 39.860 286 156 6 1 277 1 279 1.19e-63 203
MS.gene04945.t1 MTR_3g065610 40.299 268 149 5 1 259 1 266 2.34e-62 199
MS.gene04945.t1 MTR_3g080270 44.251 287 109 7 10 291 2 242 2.13e-61 195
MS.gene04945.t1 MTR_2g018940 45.703 256 123 6 20 268 24 270 4.39e-61 197
MS.gene04945.t1 MTR_8g006980 46.617 266 135 4 8 267 6 270 9.35e-60 191
MS.gene04945.t1 MTR_8g040130 47.085 223 93 5 19 233 8 213 1.25e-54 177
MS.gene04945.t1 MTR_2g095850 42.570 249 88 3 5 253 3 196 1.70e-53 173
MS.gene04945.t1 MTR_3g065850 46.696 227 114 5 9 231 7 230 8.64e-52 171
MS.gene04945.t1 MTR_3g080320 49.180 183 90 1 80 262 3 182 2.62e-51 167
MS.gene04945.t1 MTR_3g066090 57.516 153 60 2 1 149 1 152 3.98e-51 166
MS.gene04945.t1 MTR_3g065890 41.564 243 106 5 9 246 4 215 3.23e-49 163
MS.gene04945.t1 MTR_3g080350 58.696 138 55 1 19 154 63 200 3.63e-47 157
MS.gene04945.t1 MTR_2g019060 38.929 280 128 10 20 287 27 275 1.55e-46 158
MS.gene04945.t1 MTR_7g047530 40.299 268 102 7 3 268 5 216 2.28e-43 148
MS.gene04945.t1 MTR_7g451730 40.299 268 102 7 3 268 14 225 2.30e-43 148
MS.gene04945.t1 MTR_3g065730 38.431 255 124 5 11 262 8 232 6.86e-40 139
MS.gene04945.t1 MTR_3g065840 46.392 194 98 4 76 267 13 202 2.90e-36 129
MS.gene04945.t1 MTR_5g062080 38.587 184 95 3 1 177 1 173 1.44e-30 115
MS.gene04945.t1 MTR_5g062080 43.704 135 72 3 138 268 74 208 5.36e-20 87.0
MS.gene04945.t1 MTR_2g018900 34.545 220 93 4 20 234 28 201 2.35e-30 113
MS.gene04945.t1 MTR_3g065790 50.781 128 54 4 135 255 47 172 1.39e-22 92.4
MS.gene04945.t1 MTR_3g074900 27.113 284 194 7 14 289 3 281 3.51e-20 89.0
MS.gene04945.t1 MTR_3g036480 32.143 252 84 8 8 255 6 174 7.12e-20 86.3
MS.gene04945.t1 MTR_3g437220 31.301 246 103 8 8 252 7 187 2.11e-19 84.3
MS.gene04945.t1 MTR_5g098180 27.376 263 156 8 18 275 7 239 6.20e-19 84.3
MS.gene04945.t1 MTR_3g111170 39.568 139 67 3 113 250 12 134 1.21e-18 80.9
MS.gene04945.t1 MTR_4g130620 28.929 280 160 8 12 259 2 274 3.43e-18 83.2
MS.gene04945.t1 MTR_5g064480 27.419 248 169 5 21 262 10 252 1.20e-17 82.0
MS.gene04945.t1 MTR_8g044970 58.333 60 24 1 52 111 9 67 1.07e-14 68.2
MS.gene04945.t1 MTR_3g065630 41.284 109 45 2 159 267 27 116 1.95e-12 63.5
MS.gene04945.t1 MTR_8g006990 50.746 67 31 1 197 263 1 65 2.23e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04945.t1 AT4G05460 42.857 280 147 6 1 275 1 272 2.18e-58 189
MS.gene04945.t1 AT4G05475 38.462 247 140 5 20 256 39 283 1.14e-46 159
MS.gene04945.t1 AT4G05470 38.403 263 144 8 20 267 45 304 1.35e-46 159
MS.gene04945.t1 AT4G05497 38.667 225 128 4 20 238 20 240 3.46e-37 132
MS.gene04945.t1 AT4G05490 37.037 270 157 7 1 266 10 270 1.76e-36 132
MS.gene04945.t1 AT4G03630 40.860 186 106 2 84 269 31 212 2.21e-33 122
MS.gene04945.t1 AT5G52480 42.487 193 106 3 81 273 18 205 1.37e-31 118
MS.gene04945.t1 AT5G52480 40.237 169 96 3 105 273 4 167 4.22e-23 94.7
MS.gene04945.t1 AT4G08980 27.178 287 193 7 20 293 9 292 2.63e-21 92.0
MS.gene04945.t1 AT4G08980 27.178 287 193 7 20 293 9 292 2.63e-21 92.0
MS.gene04945.t1 AT4G08980 27.178 287 193 7 20 293 9 292 2.63e-21 92.0
MS.gene04945.t1 AT4G08980 27.178 287 193 7 20 293 9 292 2.63e-21 92.0
MS.gene04945.t1 AT4G08980 27.178 287 193 7 20 293 9 292 2.63e-21 92.0
MS.gene04945.t1 AT5G57900 29.151 271 154 8 20 259 7 270 1.33e-17 81.6
MS.gene04945.t1 AT3G48880 24.082 245 155 4 10 234 2 235 8.01e-14 70.9
MS.gene04945.t1 AT3G48880 24.082 245 155 4 10 234 2 235 8.01e-14 70.9
MS.gene04945.t1 AT3G48880 24.082 245 155 4 10 234 2 235 8.01e-14 70.9
MS.gene04945.t1 AT4G03635 42.353 85 47 2 101 184 4 87 6.93e-12 60.8

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTGAGCACCTTTAAAAT+AGG 0.231925 3.4:-67314952 None:intergenic
TCACATTCTGCATCCTTTGC+AGG 0.281310 3.4:-67314352 None:intergenic
TGATGGCTGTCCTCTTCTTC+AGG 0.290914 3.4:+67315113 MS.gene04945:CDS
GGTTTGCTTGCTATTCTTGA+TGG 0.309412 3.4:+67315096 MS.gene04945:CDS
CTAGGAAGTTGCAACCAATT+TGG 0.321854 3.4:-67314388 None:intergenic
TGAGATCTCGTAATCAATAT+AGG 0.336111 3.4:+67314977 MS.gene04945:CDS
GTTTGAGCACCTTTAAAATA+GGG 0.350212 3.4:-67314951 None:intergenic
ACTTCTTAGCTATGCATTCA+AGG 0.371242 3.4:-67314651 None:intergenic
GCAAATCTTTAATCTGTTCA+AGG 0.373278 3.4:-67315194 None:intergenic
CTATGCCTTCTTAATATCAT+AGG 0.379295 3.4:+67315054 MS.gene04945:CDS
TGAGTGAAAGTTTGAGGAAA+AGG 0.385400 3.4:+67315169 MS.gene04945:CDS
TGCACTTGTTACAATTTCAA+TGG 0.385596 3.4:-67314446 None:intergenic
TTAATTTGAGTGAAAGTTTG+AGG 0.394134 3.4:+67315163 MS.gene04945:CDS
AGCTTCTCTCTAAAATTCTA+TGG 0.398461 3.4:-67314831 None:intergenic
TATGCCATTGAACGTAGTTC+TGG 0.410566 3.4:+67314583 MS.gene04945:CDS
TGGCAAGCTTCAAGCATTGT+AGG 0.419877 3.4:-67314804 None:intergenic
AAAGCATAGCAGTGCCAAAT+TGG 0.436385 3.4:+67314374 MS.gene04945:CDS
CACATTCAAAATCAATCAAA+TGG 0.436729 3.4:-67315327 None:intergenic
GAGATCTCGTAATCAATATA+GGG 0.440827 3.4:+67314978 MS.gene04945:CDS
ATTTCAACTAAATCGTCATC+TGG 0.446281 3.4:-67314553 None:intergenic
CTCTAAAATTCTATGGCAGT+TGG 0.460096 3.4:-67314824 None:intergenic
TCTTCATAGTCGTAGACAAC+TGG 0.476296 3.4:-67315222 None:intergenic
ATGACCAGAACTACGTTCAA+TGG 0.508680 3.4:-67314587 None:intergenic
CCTTTAAAATAGGGCATGAT+CGG 0.508776 3.4:-67314942 None:intergenic
CATGTCAAGTTTGTCCTCAA+TGG 0.514190 3.4:+67314467 MS.gene04945:CDS
TTGCATATGTTCCACCATTG+AGG 0.521427 3.4:-67314481 None:intergenic
TGATGATCAAGTTTGTTACA+AGG 0.524209 3.4:+67315275 MS.gene04945:CDS
CATTTCCTATGATATTAAGA+AGG 0.529364 3.4:-67315059 None:intergenic
TCACTGCAAACATTCTTCAA+AGG 0.539996 3.4:+67314416 MS.gene04945:CDS
ACAGAGTCTCTTGAAGCTGT+TGG 0.542349 3.4:+67314919 MS.gene04945:CDS
ATGTTTGCAGTGATGTCTCT+AGG 0.543825 3.4:-67314406 None:intergenic
CAATATAGGGTGTATGATGT+TGG 0.545972 3.4:+67314991 MS.gene04945:CDS
GGAAATGAGCTCTCTAATGT+AGG 0.548960 3.4:+67315075 MS.gene04945:CDS
ATCTCATTCTATTCCTGCAA+AGG 0.558036 3.4:+67314339 MS.gene04945:CDS
GTCAAGTTTGTCCTCAATGG+TGG 0.565004 3.4:+67314470 MS.gene04945:CDS
ATATTAAGAGCCTGAAGAAG+AGG 0.579383 3.4:-67315123 None:intergenic
CGAATAGTTCGCCACATGAG+AGG 0.579442 3.4:-67314508 None:intergenic
CTTCAAGAGACTCTGTGGAT+AGG 0.611492 3.4:-67314912 None:intergenic
ATGTCCAGCACCTCTAGCTG+TGG 0.612238 3.4:-67314877 None:intergenic
GTATGATGTTGGTGATGATG+AGG 0.633160 3.4:+67315002 MS.gene04945:CDS
AACAGCTTCAAGAGACTCTG+TGG 0.646444 3.4:-67314917 None:intergenic
AATCGTCATCTGGAGTGTCA+AGG 0.657240 3.4:-67314543 None:intergenic
TCAATGGTGGAACATATGCA+AGG 0.664034 3.4:+67314483 MS.gene04945:CDS
TATGCAAGGATCCTCTCATG+TGG 0.693401 3.4:+67314497 MS.gene04945:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CATTTCCTATGATATTAAGA+AGG - chr3.4:67315062-67315081 None:intergenic 25.0%
!! TTAATTTGAGTGAAAGTTTG+AGG + chr3.4:67315163-67315182 MS.gene04945:CDS 25.0%
ATTTCAACTAAATCGTCATC+TGG - chr3.4:67314556-67314575 None:intergenic 30.0%
CTATGCCTTCTTAATATCAT+AGG + chr3.4:67315054-67315073 MS.gene04945:CDS 30.0%
GAGATCTCGTAATCAATATA+GGG + chr3.4:67314978-67314997 MS.gene04945:CDS 30.0%
GCAAATCTTTAATCTGTTCA+AGG - chr3.4:67315197-67315216 None:intergenic 30.0%
GTTATTGCAAAAACAATGTC+TGG + chr3.4:67315030-67315049 MS.gene04945:CDS 30.0%
TGAGATCTCGTAATCAATAT+AGG + chr3.4:67314977-67314996 MS.gene04945:CDS 30.0%
! TGCACTTGTTACAATTTCAA+TGG - chr3.4:67314449-67314468 None:intergenic 30.0%
!! AGCTTCTCTCTAAAATTCTA+TGG - chr3.4:67314834-67314853 None:intergenic 30.0%
!! AGTTTGAGCACCTTTAAAAT+AGG - chr3.4:67314955-67314974 None:intergenic 30.0%
!! GTTTGAGCACCTTTAAAATA+GGG - chr3.4:67314954-67314973 None:intergenic 30.0%
!! TGATGATCAAGTTTGTTACA+AGG + chr3.4:67315275-67315294 MS.gene04945:CDS 30.0%
ACTTCTTAGCTATGCATTCA+AGG - chr3.4:67314654-67314673 None:intergenic 35.0%
ATATTAAGAGCCTGAAGAAG+AGG - chr3.4:67315126-67315145 None:intergenic 35.0%
ATCTCATTCTATTCCTGCAA+AGG + chr3.4:67314339-67314358 MS.gene04945:CDS 35.0%
CAATATAGGGTGTATGATGT+TGG + chr3.4:67314991-67315010 MS.gene04945:CDS 35.0%
TCACTGCAAACATTCTTCAA+AGG + chr3.4:67314416-67314435 MS.gene04945:CDS 35.0%
TGAGTGAAAGTTTGAGGAAA+AGG + chr3.4:67315169-67315188 MS.gene04945:CDS 35.0%
!! CCTTTAAAATAGGGCATGAT+CGG - chr3.4:67314945-67314964 None:intergenic 35.0%
!! CTCTAAAATTCTATGGCAGT+TGG - chr3.4:67314827-67314846 None:intergenic 35.0%
AAAGCATAGCAGTGCCAAAT+TGG + chr3.4:67314374-67314393 MS.gene04945:CDS 40.0%
ATGACCAGAACTACGTTCAA+TGG - chr3.4:67314590-67314609 None:intergenic 40.0%
ATGTTTGCAGTGATGTCTCT+AGG - chr3.4:67314409-67314428 None:intergenic 40.0%
CATGTCAAGTTTGTCCTCAA+TGG + chr3.4:67314467-67314486 MS.gene04945:CDS 40.0%
CTAGGAAGTTGCAACCAATT+TGG - chr3.4:67314391-67314410 None:intergenic 40.0%
GGAAATGAGCTCTCTAATGT+AGG + chr3.4:67315075-67315094 MS.gene04945:CDS 40.0%
TATGCCATTGAACGTAGTTC+TGG + chr3.4:67314583-67314602 MS.gene04945:CDS 40.0%
TCTTCATAGTCGTAGACAAC+TGG - chr3.4:67315225-67315244 None:intergenic 40.0%
TTGCATATGTTCCACCATTG+AGG - chr3.4:67314484-67314503 None:intergenic 40.0%
! GAGAAATTTTCCACAGCTAG+AGG + chr3.4:67314867-67314886 MS.gene04945:CDS 40.0%
! TCAATGGTGGAACATATGCA+AGG + chr3.4:67314483-67314502 MS.gene04945:CDS 40.0%
!! CCGATCATGCCCTATTTTAA+AGG + chr3.4:67314942-67314961 MS.gene04945:CDS 40.0%
!! GGTTTGCTTGCTATTCTTGA+TGG + chr3.4:67315096-67315115 MS.gene04945:CDS 40.0%
!! GTATGATGTTGGTGATGATG+AGG + chr3.4:67315002-67315021 MS.gene04945:CDS 40.0%
AACAGCTTCAAGAGACTCTG+TGG - chr3.4:67314920-67314939 None:intergenic 45.0%
ACAGAGTCTCTTGAAGCTGT+TGG + chr3.4:67314919-67314938 MS.gene04945:CDS 45.0%
CTTCAAGAGACTCTGTGGAT+AGG - chr3.4:67314915-67314934 None:intergenic 45.0%
GTCAAGTTTGTCCTCAATGG+TGG + chr3.4:67314470-67314489 MS.gene04945:CDS 45.0%
TATGCAAGGATCCTCTCATG+TGG + chr3.4:67314497-67314516 MS.gene04945:CDS 45.0%
TCACATTCTGCATCCTTTGC+AGG - chr3.4:67314355-67314374 None:intergenic 45.0%
! AATCGTCATCTGGAGTGTCA+AGG - chr3.4:67314546-67314565 None:intergenic 45.0%
! TGGCAAGCTTCAAGCATTGT+AGG - chr3.4:67314807-67314826 None:intergenic 45.0%
CGAATAGTTCGCCACATGAG+AGG - chr3.4:67314511-67314530 None:intergenic 50.0%
TGATGGCTGTCCTCTTCTTC+AGG + chr3.4:67315113-67315132 MS.gene04945:CDS 50.0%
!! TTTTCCACAGCTAGAGGTGC+TGG + chr3.4:67314873-67314892 MS.gene04945:CDS 50.0%
ATGTCCAGCACCTCTAGCTG+TGG - chr3.4:67314880-67314899 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 67314334 67315338 67314334 ID=MS.gene04945
chr3.4 mRNA 67314334 67315338 67314334 ID=MS.gene04945.t1;Parent=MS.gene04945
chr3.4 exon 67314334 67314671 67314334 ID=MS.gene04945.t1.exon1;Parent=MS.gene04945.t1
chr3.4 CDS 67314334 67314671 67314334 ID=cds.MS.gene04945.t1;Parent=MS.gene04945.t1
chr3.4 exon 67314795 67315338 67314795 ID=MS.gene04945.t1.exon2;Parent=MS.gene04945.t1
chr3.4 CDS 67314795 67315338 67314795 ID=cds.MS.gene04945.t1;Parent=MS.gene04945.t1
Gene Sequence

>MS.gene04945

ATGGCATCTCATTCTATTCCTGCAAAGGATGCAGAATGTGAAAGCATAGCAGTGCCAAATTGGTTGCAACTTCCTAGAGACATCACTGCAAACATTCTTCAAAGGCTTGATACCATTGAAATTGTAACAAGTGCATGTCAAGTTTGTCCTCAATGGTGGAACATATGCAAGGATCCTCTCATGTGGCGAACTATTCGCATGATTGATGACCTTGACACTCCAGATGACGATTTAGTTGAAATTTGTCGCTATGCCATTGAACGTAGTTCTGGTCATCTAGAAATCATCGAAATTGAGTCTTTTTGCAACGACGATCTCCTTGAATGCATAGCTAAGAAGTAATTACTATTATTATCAAAATATTTTTTTTTCTCACATTAAAGTGTTAATTTCTTTTATTTATCATTTGTGATTCATTTTCTTAAAATGTTTTGTTGCTTAATGATATTATCTTTTTACAGTGCTATTAACCTACAATGCTTGAAGCTTGCCAACTGCCATAGAATTTTAGAGAGAAGCTTCTGTGAAGCTGTGAGAAATTTTCCACAGCTAGAGGTGCTGGACATTTCAGAGTGCGTCCTATCCACAGAGTCTCTTGAAGCTGTTGGCCGATCATGCCCTATTTTAAAGGTGCTCAAACTTGTGAGATCTCGTAATCAATATAGGGTGTATGATGTTGGTGATGATGAGGCATTTGTTATTGCAAAAACAATGTCTGGACTATGCCTTCTTAATATCATAGGAAATGAGCTCTCTAATGTAGGTTTGCTTGCTATTCTTGATGGCTGTCCTCTTCTTCAGGCTCTTAATATTCGAAGATGTTATAATCTTAATTTGAGTGAAAGTTTGAGGAAAAGGTGCCTTGAACAGATTAAAGATTTGCAATTGCCAGTTGTCTACGACTATGAAGACTACGATTACGATTATGATGATTACGATTATGATGATCAAGTTTGTTACAAGGATTCTCTAATAGATGATGATTGTTATGATCCATTTGATTGA

Protein sequence

>MS.gene04945.t1

MASHSIPAKDAECESIAVPNWLQLPRDITANILQRLDTIEIVTSACQVCPQWWNICKDPLMWRTIRMIDDLDTPDDDLVEICRYAIERSSGHLEIIEIESFCNDDLLECIAKNAINLQCLKLANCHRILERSFCEAVRNFPQLEVLDISECVLSTESLEAVGRSCPILKVLKLVRSRNQYRVYDVGDDEAFVIAKTMSGLCLLNIIGNELSNVGLLAILDGCPLLQALNIRRCYNLNLSESLRKRCLEQIKDLQLPVVYDYEDYDYDYDDYDYDDQVCYKDSLIDDDCYDPFD