Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049453.t1 | XP_003599280.1 | 86.5 | 170 | 23 | 0 | 1 | 170 | 1 | 170 | 1.30E-73 | 285.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049453.t1 | G7IWW8 | 86.5 | 170 | 23 | 0 | 1 | 170 | 1 | 170 | 9.4e-74 | 285.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004251 | MS.gene049453 | PPI |
| MS.gene01564 | MS.gene049453 | PPI |
| MS.gene54297 | MS.gene049453 | PPI |
| MS.gene049453 | MS.gene002580 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049453.t1 | MTR_3g031140 | 98.235 | 170 | 3 | 0 | 1 | 170 | 1 | 170 | 8.85e-122 | 340 |
| MS.gene049453.t1 | MTR_5g074290 | 65.116 | 172 | 44 | 4 | 1 | 170 | 1 | 158 | 9.30e-62 | 188 |
| MS.gene049453.t1 | MTR_2g016150 | 43.333 | 180 | 91 | 3 | 1 | 169 | 1 | 180 | 9.09e-33 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049453.t1 | AT2G30230 | 55.435 | 184 | 59 | 3 | 1 | 170 | 1 | 175 | 4.00e-59 | 182 |
| MS.gene049453.t1 | AT1G06980 | 55.618 | 178 | 60 | 2 | 1 | 170 | 1 | 167 | 1.35e-56 | 175 |
| MS.gene049453.t1 | AT1G29195 | 43.915 | 189 | 85 | 5 | 1 | 169 | 1 | 188 | 2.25e-45 | 147 |
Find 42 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAACAACGCCTTGAGAACT+TGG | 0.264820 | 3.2:-56561731 | None:intergenic |
| AGAAAGGTCGTGTTGGAATA+TGG | 0.306669 | 3.2:+56562029 | MS.gene049453:CDS |
| AACATCACCCTTGCAATATA+AGG | 0.321491 | 3.2:+56561979 | MS.gene049453:CDS |
| TGGCTTGTGTTCTTCCTTGT+GGG | 0.333742 | 3.2:+56561597 | MS.gene049453:CDS |
| TAATAGCTTAAGGTGTTGTT+TGG | 0.366641 | 3.2:+56561577 | MS.gene049453:CDS |
| GGTTTGCTTAGAACATGGTT+AGG | 0.379565 | 3.2:-56561710 | None:intergenic |
| AGCCATTCAAATGCACTATT+CGG | 0.415539 | 3.2:-56561634 | None:intergenic |
| GATCCCGGCAACATCTTTGC+CGG | 0.428218 | 3.2:+56561811 | MS.gene049453:CDS |
| ATAATATGGGTAATAGCTTA+AGG | 0.429370 | 3.2:+56561567 | None:intergenic |
| GTTTGCTTAGAACATGGTTA+GGG | 0.435444 | 3.2:-56561709 | None:intergenic |
| GAACATGGTTAGGGTTTGCT+TGG | 0.435589 | 3.2:-56561700 | None:intergenic |
| CATCTTTGCCGGAGAACAAA+AGG | 0.452972 | 3.2:+56561822 | MS.gene049453:CDS |
| TTCTCCGGCAAAGATGTTGC+CGG | 0.464422 | 3.2:-56561815 | None:intergenic |
| TCAAGACGGGATCGCAAGAA+AGG | 0.467249 | 3.2:+56562013 | MS.gene049453:CDS |
| TTTCTTCTCCTTATATTGCA+AGG | 0.484846 | 3.2:-56561987 | None:intergenic |
| GATGGTGATGATGACAATAA+TGG | 0.485742 | 3.2:+56561926 | MS.gene049453:CDS |
| TTGGCTTGTGTTCTTCCTTG+TGG | 0.486689 | 3.2:+56561596 | MS.gene049453:CDS |
| GTTCTCAAGGCGTTGTTCGT+CGG | 0.495768 | 3.2:+56561735 | MS.gene049453:CDS |
| GACAATAATGGTAGTGGTGA+TGG | 0.499039 | 3.2:+56561938 | MS.gene049453:CDS |
| ATCACCATCATCACCGTGCT+TGG | 0.502755 | 3.2:-56561912 | None:intergenic |
| AGCACCAAGCACGGTGATGA+TGG | 0.505783 | 3.2:+56561908 | MS.gene049453:CDS |
| TTCTTCTCCTTATATTGCAA+GGG | 0.509390 | 3.2:-56561986 | None:intergenic |
| CCTCTCTTGAGCTCAGTCTC+CGG | 0.511219 | 3.2:-56561773 | None:intergenic |
| ATCCGAATAGTGCATTTGAA+TGG | 0.519987 | 3.2:+56561632 | MS.gene049453:CDS |
| GGCTTGTGTTCTTCCTTGTG+GGG | 0.520338 | 3.2:+56561598 | MS.gene049453:CDS |
| TCTCCGGCAAAGATGTTGCC+GGG | 0.532431 | 3.2:-56561814 | None:intergenic |
| GGAGAAGAAATCCTCAAGAC+GGG | 0.545214 | 3.2:+56562000 | MS.gene049453:CDS |
| TTTCTTGCGATCCCGTCTTG+AGG | 0.547201 | 3.2:-56562011 | None:intergenic |
| TCAGAAATACTTGCTAAATG+AGG | 0.557315 | 3.2:-56562055 | None:intergenic |
| TTGCCGGAGAACAAAAGGAA+TGG | 0.573260 | 3.2:+56561827 | MS.gene049453:CDS |
| CTAAGCAAACCAAGTTCTCA+AGG | 0.584166 | 3.2:+56561722 | MS.gene049453:CDS |
| AAGCATATACTTCTTGATCC+CGG | 0.589215 | 3.2:+56561796 | MS.gene049453:CDS |
| AGGAGAAGAAATCCTCAAGA+CGG | 0.590285 | 3.2:+56561999 | MS.gene049453:CDS |
| GATGATGACAATAATGGTAG+TGG | 0.590474 | 3.2:+56561932 | MS.gene049453:CDS |
| AACTTGGTTTGCTTAGAACA+TGG | 0.593833 | 3.2:-56561715 | None:intergenic |
| GATCGCAAGAAAGGTCGTGT+TGG | 0.602795 | 3.2:+56562022 | MS.gene049453:CDS |
| CCGGAGACTGAGCTCAAGAG+AGG | 0.605430 | 3.2:+56561773 | MS.gene049453:CDS |
| ATTACACATTCAATCACAGC+AGG | 0.608213 | 3.2:+56561668 | MS.gene049453:CDS |
| AAGAAAAGAAGCACCAAGCA+CGG | 0.616563 | 3.2:+56561899 | MS.gene049453:CDS |
| ATCAAATCTAGTGCCCCACA+AGG | 0.641179 | 3.2:-56561611 | None:intergenic |
| ACATTCAATCACAGCAGGTG+AGG | 0.650445 | 3.2:+56561673 | MS.gene049453:CDS |
| AAGGTCGTGTTGGAATATGG+AGG | 0.705859 | 3.2:+56562032 | MS.gene049453:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCTTCTCCTTATATTGCAA+GGG | - | chr3.2:56561989-56562008 | None:intergenic | 30.0% | |
| TTTCTTCTCCTTATATTGCA+AGG | - | chr3.2:56561990-56562009 | None:intergenic | 30.0% | |
| ! | TCAGAAATACTTGCTAAATG+AGG | - | chr3.2:56562058-56562077 | None:intergenic | 30.0% |
| !! | TAATAGCTTAAGGTGTTGTT+TGG | + | chr3.2:56561577-56561596 | MS.gene049453:CDS | 30.0% |
| AACATCACCCTTGCAATATA+AGG | + | chr3.2:56561979-56561998 | MS.gene049453:CDS | 35.0% | |
| AAGCATATACTTCTTGATCC+CGG | + | chr3.2:56561796-56561815 | MS.gene049453:CDS | 35.0% | |
| AGCCATTCAAATGCACTATT+CGG | - | chr3.2:56561637-56561656 | None:intergenic | 35.0% | |
| ATCCGAATAGTGCATTTGAA+TGG | + | chr3.2:56561632-56561651 | MS.gene049453:CDS | 35.0% | |
| ATTACACATTCAATCACAGC+AGG | + | chr3.2:56561668-56561687 | MS.gene049453:CDS | 35.0% | |
| GATGATGACAATAATGGTAG+TGG | + | chr3.2:56561932-56561951 | MS.gene049453:CDS | 35.0% | |
| GTTTGCTTAGAACATGGTTA+GGG | - | chr3.2:56561712-56561731 | None:intergenic | 35.0% | |
| ! | GATGGTGATGATGACAATAA+TGG | + | chr3.2:56561926-56561945 | MS.gene049453:CDS | 35.0% |
| !! | AACTTGGTTTGCTTAGAACA+TGG | - | chr3.2:56561718-56561737 | None:intergenic | 35.0% |
| !! | TCGGATTTTGATCATTTCAC+CGG | + | chr3.2:56561754-56561773 | MS.gene049453:CDS | 35.0% |
| AGAAAGGTCGTGTTGGAATA+TGG | + | chr3.2:56562029-56562048 | MS.gene049453:CDS | 40.0% | |
| AGGAGAAGAAATCCTCAAGA+CGG | + | chr3.2:56561999-56562018 | MS.gene049453:CDS | 40.0% | |
| CTAAGCAAACCAAGTTCTCA+AGG | + | chr3.2:56561722-56561741 | MS.gene049453:CDS | 40.0% | |
| ! | GATCCATTCCTTTTGTTCTC+CGG | - | chr3.2:56561833-56561852 | None:intergenic | 40.0% |
| ! | GGTTTGCTTAGAACATGGTT+AGG | - | chr3.2:56561713-56561732 | None:intergenic | 40.0% |
| !! | AAGAAAAGAAGCACCAAGCA+CGG | + | chr3.2:56561899-56561918 | MS.gene049453:CDS | 40.0% |
| !! | GACAATAATGGTAGTGGTGA+TGG | + | chr3.2:56561938-56561957 | MS.gene049453:CDS | 40.0% |
| AAGGTCGTGTTGGAATATGG+AGG | + | chr3.2:56562032-56562051 | MS.gene049453:CDS | 45.0% | |
| ACATTCAATCACAGCAGGTG+AGG | + | chr3.2:56561673-56561692 | MS.gene049453:CDS | 45.0% | |
| ATCAAATCTAGTGCCCCACA+AGG | - | chr3.2:56561614-56561633 | None:intergenic | 45.0% | |
| CATCTTTGCCGGAGAACAAA+AGG | + | chr3.2:56561822-56561841 | MS.gene049453:CDS | 45.0% | |
| GGAGAAGAAATCCTCAAGAC+GGG | + | chr3.2:56562000-56562019 | MS.gene049453:CDS | 45.0% | |
| TTGCCGGAGAACAAAAGGAA+TGG | + | chr3.2:56561827-56561846 | MS.gene049453:CDS | 45.0% | |
| ! | GAACATGGTTAGGGTTTGCT+TGG | - | chr3.2:56561703-56561722 | None:intergenic | 45.0% |
| ! | TGGCTTGTGTTCTTCCTTGT+GGG | + | chr3.2:56561597-56561616 | MS.gene049453:CDS | 45.0% |
| !! | TTGGCTTGTGTTCTTCCTTG+TGG | + | chr3.2:56561596-56561615 | MS.gene049453:CDS | 45.0% |
| ATCACCATCATCACCGTGCT+TGG | - | chr3.2:56561915-56561934 | None:intergenic | 50.0% | |
| CGAACAACGCCTTGAGAACT+TGG | - | chr3.2:56561734-56561753 | None:intergenic | 50.0% | |
| GATCGCAAGAAAGGTCGTGT+TGG | + | chr3.2:56562022-56562041 | MS.gene049453:CDS | 50.0% | |
| GGCTTGTGTTCTTCCTTGTG+GGG | + | chr3.2:56561598-56561617 | MS.gene049453:CDS | 50.0% | |
| TCAAGACGGGATCGCAAGAA+AGG | + | chr3.2:56562013-56562032 | MS.gene049453:CDS | 50.0% | |
| TTCTCCGGCAAAGATGTTGC+CGG | - | chr3.2:56561818-56561837 | None:intergenic | 50.0% | |
| TTTCTTGCGATCCCGTCTTG+AGG | - | chr3.2:56562014-56562033 | None:intergenic | 50.0% | |
| !! | GTTCTCAAGGCGTTGTTCGT+CGG | + | chr3.2:56561735-56561754 | MS.gene049453:CDS | 50.0% |
| AGCACCAAGCACGGTGATGA+TGG | + | chr3.2:56561908-56561927 | MS.gene049453:CDS | 55.0% | |
| CCTCTCTTGAGCTCAGTCTC+CGG | - | chr3.2:56561776-56561795 | None:intergenic | 55.0% | |
| GATCCCGGCAACATCTTTGC+CGG | + | chr3.2:56561811-56561830 | MS.gene049453:CDS | 55.0% | |
| TCTCCGGCAAAGATGTTGCC+GGG | - | chr3.2:56561817-56561836 | None:intergenic | 55.0% | |
| CCGGAGACTGAGCTCAAGAG+AGG | + | chr3.2:56561773-56561792 | MS.gene049453:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 56561572 | 56562084 | 56561572 | ID=MS.gene049453 |
| chr3.2 | mRNA | 56561572 | 56562084 | 56561572 | ID=MS.gene049453.t1;Parent=MS.gene049453 |
| chr3.2 | exon | 56561572 | 56562084 | 56561572 | ID=MS.gene049453.t1.exon1;Parent=MS.gene049453.t1 |
| chr3.2 | CDS | 56561572 | 56562084 | 56561572 | ID=cds.MS.gene049453.t1;Parent=MS.gene049453.t1 |
| Gene Sequence |
| Protein sequence |
