AlfalfaGEDB | MS.gene050087

M. sativa cultivar XinJiangDaYe / MS.gene050087

KEGG: -
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050087.t1	XP_013455967.1	89.7	136	12	1	1	134	1	136	2.70E-61	244.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050087.t1	P0DKL1	42.1	76	41	2	16	91	18	90	3.5e-09	62.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050087.t1	A0A072ULQ3	89.7	136	12	1	1	134	1	136	1.9e-61	244.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene050087	MS.gene22418	PPI
MS.gene050087	MS.gene20078	PPI
MS.gene050087	MS.gene007530	PPI
MS.gene050087	MS.gene007417	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050087.t1	MTR_4g057800	89.706	136	12	1	1	134	1	136	6.84e-87	249
MS.gene050087.t1	MTR_4g057810	83.333	108	18	0	1	108	1	108	1.61e-64	192
MS.gene050087.t1	MTR_2g095430	56.190	105	40	2	5	107	16	116	1.69e-36	122
MS.gene050087.t1	MTR_1g063330	46.667	75	40	0	26	100	20	94	1.52e-20	80.5
MS.gene050087.t1	MTR_1g063340	45.333	75	41	0	26	100	20	94	9.20e-20	78.6
MS.gene050087.t1	MTR_1g063300	44.318	88	49	0	13	100	14	101	1.01e-19	78.6
MS.gene050087.t1	MTR_1g063290	42.045	88	51	0	13	100	14	101	3.08e-19	77.4
MS.gene050087.t1	MTR_1g063310	41.772	79	46	0	33	111	21	99	5.10e-18	73.9
MS.gene050087.t1	MTR_1g063280	50.746	67	33	0	34	100	38	104	2.90e-17	72.4
MS.gene050087.t1	MTR_1g063260	50.746	67	33	0	34	100	37	103	5.62e-17	71.6
MS.gene050087.t1	MTR_7g101920	43.836	73	41	0	33	105	30	102	1.83e-16	70.5
MS.gene050087.t1	MTR_7g101890	44.444	63	35	0	38	100	52	114	1.18e-14	65.9
MS.gene050087.t1	MTR_8g076040	43.243	74	40	1	26	99	29	100	1.83e-14	65.1
MS.gene050087.t1	MTR_5g021820	45.455	66	34	1	26	91	28	91	2.90e-14	64.7
MS.gene050087.t1	MTR_3g117610	54.545	55	23	1	37	91	44	96	3.30e-13	62.0
MS.gene050087.t1	MTR_4g072190	43.902	82	38	3	11	91	13	87	3.94e-13	61.6
MS.gene050087.t1	MTR_3g113310	46.032	63	32	1	37	99	41	101	2.08e-12	59.7
MS.gene050087.t1	MTR_4g072910	36.364	88	46	3	6	92	10	88	3.30e-12	59.3
MS.gene050087.t1	MTR_3g117640	51.786	56	25	2	37	91	33	87	6.97e-11	55.8
MS.gene050087.t1	MTR_4g124750	36.458	96	58	2	5	100	37	129	9.35e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050087.t1	AT2G28085	46.296	108	51	3	4	104	16	123	1.53e-24	91.7
MS.gene050087.t1	AT3G09870	46.269	67	36	0	34	100	41	107	1.19e-15	68.6
MS.gene050087.t1	AT2G21220	56.364	55	22	1	37	91	39	91	1.20e-13	63.2
MS.gene050087.t1	AT4G34760	45.070	71	37	1	29	99	34	102	2.14e-12	59.7
MS.gene050087.t1	AT4G34780	46.667	60	30	2	37	94	27	86	4.28e-12	58.9
MS.gene050087.t1	AT4G38860	48.438	64	30	2	29	91	31	92	5.70e-12	58.5
MS.gene050087.t1	AT5G53590	37.662	77	43	2	37	110	45	119	7.50e-12	59.3
MS.gene050087.t1	AT3G61900	43.548	62	34	1	31	91	23	84	9.74e-12	58.9
MS.gene050087.t1	AT1G19830	43.478	69	37	1	37	105	47	113	1.70e-11	57.8
MS.gene050087.t1	AT4G34800	43.421	76	41	2	27	100	10	85	1.77e-11	57.0
MS.gene050087.t1	AT1G75580	50.909	55	25	1	37	91	43	95	2.40e-11	57.0
MS.gene050087.t1	AT5G66260	44.444	63	33	1	38	100	34	94	2.55e-11	57.0
MS.gene050087.t1	AT2G37030	41.270	63	35	1	30	92	41	101	3.15e-11	57.4
MS.gene050087.t1	AT4G12410	39.189	74	41	2	19	91	60	130	6.26e-11	57.0

Find 0 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAGAAATGATTATAAATCA+AGG	-	33213:17537-17556	MS.gene050087:CDS	20.0%
!!	TAATAAACTTCAGATGTTAT+TGG	-	33213:17742-17761	MS.gene050087:CDS	20.0%
!!!	AATAACATCTGAAGTTTATT+AGG	+	33213:17743-17762	None:intergenic	20.0%
!!!	ATAACATCTGAAGTTTATTA+GGG	+	33213:17742-17761	None:intergenic	20.0%
!	AATCATTTCTTCCCATTAAT+AGG	+	33213:17529-17548	None:intergenic	25.0%
!	ATCATTTCTTCCCATTAATA+GGG	+	33213:17528-17547	None:intergenic	25.0%
!	TGATTATAAATCAAGGTATG+TGG	-	33213:17544-17563	MS.gene050087:CDS	25.0%
!!!	TTTAACTTGAAATATCCACT+CGG	+	33213:17836-17855	None:intergenic	25.0%
	AAGTATGGATTTAATGGAGA+TGG	-	33213:17695-17714	MS.gene050087:CDS	30.0%
	CCAATAGACTCACAAATATT+GGG	+	33213:17661-17680	None:intergenic	30.0%
	CTCCAAAAAACCCTATTAAT+GGG	-	33213:17515-17534	MS.gene050087:CDS	30.0%
	TCCAATAGACTCACAAATAT+TGG	+	33213:17662-17681	None:intergenic	30.0%
!	GCTGAAAAGTATGGATTTAA+TGG	-	33213:17689-17708	MS.gene050087:CDS	30.0%
!!!	TTCCCATTAATAGGGTTTTT+TGG	+	33213:17520-17539	None:intergenic	30.0%
	CCCAATATTTGTGAGTCTAT+TGG	-	33213:17658-17677	MS.gene050087:CDS	35.0%
	CCTCCAAAAAACCCTATTAA+TGG	-	33213:17514-17533	MS.gene050087:CDS	35.0%
	TAAATCAAGGTATGTGGCAA+AGG	-	33213:17550-17569	MS.gene050087:CDS	35.0%
	TGAAGTTTATTAGGGCTACA+AGG	+	33213:17734-17753	None:intergenic	35.0%
!	GAAAAAGGGACATTTTGTTG+TGG	-	33213:17577-17596	MS.gene050087:CDS	35.0%
!!	ACATTTTGTTGTGGTTGCTA+AGG	-	33213:17586-17605	MS.gene050087:CDS	35.0%
!!	GGGTTTGTTAAACAACTCAA+TGG	+	33213:17641-17660	None:intergenic	35.0%
!!!	CCATTAATAGGGTTTTTTGG+AGG	+	33213:17517-17536	None:intergenic	35.0%
	GAACAAGCAGCTGAAAAGTA+TGG	-	33213:17680-17699	MS.gene050087:CDS	40.0%
	GTGGCAAAGGATGTGAAAAA+GGG	-	33213:17563-17582	MS.gene050087:CDS	40.0%
	TGTGGCAAAGGATGTGAAAA+AGG	-	33213:17562-17581	MS.gene050087:CDS	40.0%
	AATATCCACTCGGTCGTCTT+TGG	+	33213:17826-17845	None:intergenic	45.0%
	GTTATTGGTGCAGCAATTGC+AGG	-	33213:17757-17776	MS.gene050087:CDS	45.0%
	GTGCAGCAATTGCAGGAAGA+AGG	-	33213:17764-17783	MS.gene050087:CDS	50.0%
	TGTTACCAAAGACGACCGAG+TGG	-	33213:17818-17837	MS.gene050087:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
33213	gene	17464	17877	17464	ID=MS.gene050087
33213	mRNA	17464	17877	17464	ID=MS.gene050087.t1;Parent=MS.gene050087
33213	exon	17464	17877	17464	ID=MS.gene050087.t1.exon1;Parent=MS.gene050087.t1
33213	CDS	17464	17877	17464	ID=cds.MS.gene050087.t1;Parent=MS.gene050087.t1

Gene Sequence

>MS.gene050087

ATGGTTGTTGTAGGTGGAATTTTGAAGCTCAAGAATAATGTCTTCAAAAACCTCCAAAAAACCCTATTAATGGGAAGAAATGATTATAAATCAAGGTATGTGGCAAAGGATGTGAAAAAGGGACATTTTGTTGTGGTTGCTAAGGATGAAGATGAAGCAAAGAGATTTATTGTGCCATTGAGTTGTTTAACAAACCCAATATTTGTGAGTCTATTGGAACAAGCAGCTGAAAAGTATGGATTTAATGGAGATGGTGCACTAACTATTCCTTGTAGCCCTAATAAACTTCAGATGTTATTGGTGCAGCAATTGCAGGAAGAAGGAAGCTATTGCTCTGATCAAGTCATGTTTAAATGTTACCAAAGACGACCGAGTGGATATTTCAAGTTAAAGCAAATTGTATCTCAACATTAA

Protein sequence

>MS.gene050087.t1

MVVVGGILKLKNNVFKNLQKTLLMGRNDYKSRYVAKDVKKGHFVVVAKDEDEAKRFIVPLSCLTNPIFVSLLEQAAEKYGFNGDGALTIPCSPNKLQMLLVQQLQEEGSYCSDQVMFKCYQRRPSGYFKLKQIVSQH