AlfalfaGEDB | MS.gene050089

M. sativa cultivar XinJiangDaYe / MS.gene050089

KEGG: -
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050089.t1	XP_013455967.1	84.4	135	19	1	1	133	1	135	1.20E-56	229.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050089.t1	Q9ZUZ3	32.9	82	54	1	19	99	5	86	2.3e-08	60.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050089.t1	A0A072ULQ3	84.4	135	19	1	1	133	1	135	8.5e-57	229.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene050089	MS.gene007530	PPI
MS.gene050089	MS.gene007417	PPI
MS.gene050089	MS.gene22418	PPI
MS.gene050089	MS.gene20078	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050089.t1	MTR_4g057800	83.824	136	20	1	1	134	1	136	3.56e-81	235
MS.gene050089.t1	MTR_4g057810	86.111	108	15	0	1	108	1	108	1.91e-66	197
MS.gene050089.t1	MTR_2g095430	56.190	105	40	2	5	107	16	116	1.81e-36	122
MS.gene050089.t1	MTR_1g063300	46.591	88	47	0	13	100	14	101	7.02e-21	81.6
MS.gene050089.t1	MTR_1g063290	51.389	72	35	0	29	100	30	101	1.07e-20	81.3
MS.gene050089.t1	MTR_1g063280	54.545	66	30	0	35	100	39	104	3.73e-19	77.4
MS.gene050089.t1	MTR_1g063330	46.667	75	40	0	26	100	20	94	4.15e-19	76.6
MS.gene050089.t1	MTR_1g063340	46.667	75	40	0	26	100	20	94	4.47e-19	76.6
MS.gene050089.t1	MTR_1g063260	54.545	66	30	0	35	100	38	103	7.14e-19	76.6
MS.gene050089.t1	MTR_1g063310	45.882	85	43	2	16	100	7	88	1.00e-18	75.9
MS.gene050089.t1	MTR_7g101920	43.836	73	41	0	33	105	30	102	6.87e-17	71.6
MS.gene050089.t1	MTR_7g101890	46.377	69	37	0	38	106	52	120	1.16e-16	71.2
MS.gene050089.t1	MTR_7g118010	34.722	72	47	0	28	99	13	84	3.53e-12	59.3
MS.gene050089.t1	MTR_3g092220	30.137	73	51	0	27	99	22	94	6.24e-12	58.9
MS.gene050089.t1	MTR_3g117610	48.214	56	27	1	37	92	44	97	1.11e-11	58.2
MS.gene050089.t1	MTR_4g072910	35.632	87	46	3	6	91	10	87	1.47e-11	57.4
MS.gene050089.t1	MTR_4g072190	39.759	83	42	3	11	92	13	88	1.56e-11	57.4
MS.gene050089.t1	MTR_8g076040	40.909	66	37	1	26	91	29	92	1.74e-11	57.4
MS.gene050089.t1	MTR_5g021820	39.394	66	38	1	26	91	28	91	2.19e-11	57.0
MS.gene050089.t1	MTR_4g072160	38.144	97	54	3	4	100	7	97	2.29e-11	57.8
MS.gene050089.t1	MTR_0388s0020	40.625	64	37	1	37	99	74	137	7.43e-11	56.6
MS.gene050089.t1	MTR_4g072570	34.524	84	52	2	17	99	4	85	8.78e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050089.t1	AT2G28085	46.296	108	51	3	4	104	16	123	1.10e-23	89.4
MS.gene050089.t1	AT3G09870	50.000	64	32	0	37	100	44	107	5.16e-16	69.3
MS.gene050089.t1	AT5G53590	37.662	77	43	2	37	110	45	119	3.56e-12	60.1
MS.gene050089.t1	AT2G21220	50.909	55	25	1	37	91	39	91	4.77e-12	58.9
MS.gene050089.t1	AT3G61900	38.710	62	37	1	31	91	23	84	1.40e-11	58.5
MS.gene050089.t1	AT4G34800	44.118	68	36	2	27	92	10	77	3.46e-11	56.2
MS.gene050089.t1	AT1G19830	33.654	104	66	2	2	105	13	113	4.74e-11	56.6
MS.gene050089.t1	AT4G34790	41.429	70	39	2	38	105	39	108	6.84e-11	55.8
MS.gene050089.t1	AT2G46690	32.927	82	54	1	19	99	5	86	7.49e-11	56.2
MS.gene050089.t1	AT4G12410	32.927	82	51	2	19	99	60	138	7.76e-11	57.0
MS.gene050089.t1	AT4G34780	43.860	57	30	2	37	91	27	83	8.63e-11	55.8

Find 17 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACATTTCTTCCCATTAAT+AGG	0.197143	4.2:-45537531	None:intergenic
TCTAATAGACTCACAAATAT+TGG	0.237003	4.2:-45537664	None:intergenic
AACATTTCTTCCCATTAATA+GGG	0.284308	4.2:-45537530	None:intergenic
AATATCATTTGAAGTTCATT+AGG	0.324508	4.2:-45537745	None:intergenic
TGCGGCACAAGATGTGAAAA+AGG	0.332268	4.2:+45537567	MS.gene050089:CDS
AATATCCACTAGGTCGTCTT+TGG	0.338952	4.2:-45537828	None:intergenic
GATATTAGTGCAGCAATTGC+AGG	0.415449	4.2:+45537762	MS.gene050089:CDS
AAATATGGATTTAATGGAAA+TGG	0.431922	4.2:+45537700	MS.gene050089:CDS
GAAATCAAAATGGTTATTGT+AGG	0.436403	4.2:+45537460	None:intergenic
GGATTTGTTAAACAACTCAA+TGG	0.477349	4.2:-45537643	None:intergenic
ATCAAAATGGTTATTGTAGG+TGG	0.487491	4.2:+45537463	None:intergenic
GAAGAAATGTTCATAAATCA+AGG	0.506031	4.2:+45537542	MS.gene050089:CDS
GTGCAGCAATTGCAGGATGA+AGG	0.533580	4.2:+45537769	MS.gene050089:CDS
TGAAGTTCATTAGGTCGACA+AGG	0.625993	4.2:-45537736	None:intergenic
TTTAAATTGAAATATCCACT+AGG	0.648424	4.2:-45537838	None:intergenic
TGTTCATAAATCAAGGTATG+CGG	0.650933	4.2:+45537549	MS.gene050089:CDS
TGTTACCAAAGACGACCTAG+TGG	0.673212	4.2:+45537823	MS.gene050089:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATATGGATTTAATGGAAA+TGG	+	chr4.2:45537700-45537719	MS.gene050089:CDS	20.0%
!!	TTTAAATTGAAATATCCACT+AGG	-	chr4.2:45537841-45537860	None:intergenic	20.0%
!!!	AATATCATTTGAAGTTCATT+AGG	-	chr4.2:45537748-45537767	None:intergenic	20.0%
!	AACATTTCTTCCCATTAATA+GGG	-	chr4.2:45537533-45537552	None:intergenic	25.0%
!	GAAGAAATGTTCATAAATCA+AGG	+	chr4.2:45537542-45537561	MS.gene050089:CDS	25.0%
!	GCTGAAAAATATGGATTTAA+TGG	+	chr4.2:45537694-45537713	MS.gene050089:CDS	25.0%
!	TCTAATAGACTCACAAATAT+TGG	-	chr4.2:45537667-45537686	None:intergenic	25.0%
	CTCCAAAAAACCCTATTAAT+GGG	+	chr4.2:45537520-45537539	MS.gene050089:CDS	30.0%
	GAACATTTCTTCCCATTAAT+AGG	-	chr4.2:45537534-45537553	None:intergenic	30.0%
	TGTTCATAAATCAAGGTATG+CGG	+	chr4.2:45537549-45537568	MS.gene050089:CDS	30.0%
!!	ACATTTTGTTGTGATTGCTA+AGG	+	chr4.2:45537591-45537610	MS.gene050089:CDS	30.0%
!!	GGATTTGTTAAACAACTCAA+TGG	-	chr4.2:45537646-45537665	None:intergenic	30.0%
!!!	TTCCCATTAATAGGGTTTTT+TGG	-	chr4.2:45537525-45537544	None:intergenic	30.0%
	GAACAAGCAGCTGAAAAATA+TGG	+	chr4.2:45537685-45537704	MS.gene050089:CDS	35.0%
	GCTCCAAAAAACCCTATTAA+TGG	+	chr4.2:45537519-45537538	MS.gene050089:CDS	35.0%
	AATATCCACTAGGTCGTCTT+TGG	-	chr4.2:45537831-45537850	None:intergenic	40.0%
	GATATTAGTGCAGCAATTGC+AGG	+	chr4.2:45537762-45537781	MS.gene050089:CDS	40.0%
	TGAAGTTCATTAGGTCGACA+AGG	-	chr4.2:45537739-45537758	None:intergenic	40.0%
	GCGGCACAAGATGTGAAAAA+GGG	+	chr4.2:45537568-45537587	MS.gene050089:CDS	45.0%
	TGCGGCACAAGATGTGAAAA+AGG	+	chr4.2:45537567-45537586	MS.gene050089:CDS	45.0%
	TGTTACCAAAGACGACCTAG+TGG	+	chr4.2:45537823-45537842	MS.gene050089:CDS	45.0%
	GTGCAGCAATTGCAGGATGA+AGG	+	chr4.2:45537769-45537788	MS.gene050089:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	45537469	45537885	45537469	ID=MS.gene050089
chr4.2	mRNA	45537469	45537885	45537469	ID=MS.gene050089.t1;Parent=MS.gene050089
chr4.2	exon	45537469	45537885	45537469	ID=MS.gene050089.t1.exon1;Parent=MS.gene050089.t1
chr4.2	CDS	45537469	45537885	45537469	ID=cds.MS.gene050089.t1;Parent=MS.gene050089.t1

Gene Sequence

>MS.gene050089

ATGGTTATTGTAGGTGGAATTTTGAAGCTCAAGAATAATGTCTTCAAAAAGCTCCAAAAAACCCTATTAATGGGAAGAAATGTTCATAAATCAAGGTATGCGGCACAAGATGTGAAAAAGGGACATTTTGTTGTGATTGCTAAGGATGAAGATGAAGCAAAAAGATTTATTGTGCCATTGAGTTGTTTAACAAATCCAATATTTGTGAGTCTATTAGAACAAGCAGCTGAAAAATATGGATTTAATGGAAATGGTGCAGTTACAGTTCCTTGTCGACCTAATGAACTTCAAATGATATTAGTGCAGCAATTGCAGGATGAAGGAAGCTATTTCTCTGATCAAGTCATGTTTAAATGTTACCAAAGACGACCTAGTGGATATTTCAATTTAAAGCATATTACATCTAGAGATTATTAG

Protein sequence

>MS.gene050089.t1

MVIVGGILKLKNNVFKKLQKTLLMGRNVHKSRYAAQDVKKGHFVVIAKDEDEAKRFIVPLSCLTNPIFVSLLEQAAEKYGFNGNGAVTVPCRPNELQMILVQQLQDEGSYFSDQVMFKCYQRRPSGYFNLKHITSRDY