AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051219.t1 MTR_2g040160 76.300 654 129 5 2 633 839 1488 0.0 1009
MS.gene051219.t1 MTR_2g040220 76.708 644 120 6 1 630 771 1398 0.0 1004
MS.gene051219.t1 MTR_2g040260 74.924 654 135 6 2 633 771 1417 0.0 991
MS.gene051219.t1 MTR_2g040370 76.124 645 133 6 2 632 770 1407 0.0 991
MS.gene051219.t1 MTR_2g040370 79.144 374 75 2 243 616 1406 1776 0.0 604
MS.gene051219.t1 MTR_2g040370 76.124 645 133 6 2 632 770 1407 0.0 990
MS.gene051219.t1 MTR_2g040370 79.144 374 75 2 243 616 1406 1776 0.0 604
MS.gene051219.t1 MTR_2g040350 74.189 647 151 4 2 633 776 1421 0.0 966
MS.gene051219.t1 MTR_2g040350 74.189 647 151 4 2 633 558 1203 0.0 966
MS.gene051219.t1 MTR_7g099680 82.653 392 62 3 245 634 4 391 0.0 652
MS.gene051219.t1 MTR_7g024390 81.633 392 66 3 245 634 4 391 0.0 649
MS.gene051219.t1 MTR_7g024580 80.867 392 69 3 245 634 4 391 0.0 639
MS.gene051219.t1 MTR_4g063710 80.612 392 70 3 245 634 4 391 0.0 636
MS.gene051219.t1 MTR_4g063720 80.357 392 71 3 245 634 4 391 0.0 632
MS.gene051219.t1 MTR_4g130540 77.577 388 81 3 247 632 5 388 0.0 604
MS.gene051219.t1 MTR_5g066800 67.164 402 119 4 244 633 3 403 0.0 549
MS.gene051219.t1 MTR_7g026030 67.179 390 120 4 244 629 3 388 0.0 540
MS.gene051219.t1 MTR_7g021440 66.921 393 122 4 242 630 1 389 0.0 539
MS.gene051219.t1 MTR_2g038260 66.497 394 124 4 244 633 3 392 0.0 537
MS.gene051219.t1 MTR_2g437440 67.179 390 120 4 247 632 6 391 0.0 535
MS.gene051219.t1 MTR_7g025760 65.985 391 125 4 244 630 3 389 0.0 534
MS.gene051219.t1 MTR_7g025820 67.436 390 119 4 244 629 3 388 0.0 534
MS.gene051219.t1 MTR_8g032650 67.775 391 118 4 244 630 3 389 0.0 533
MS.gene051219.t1 MTR_2g032550 66.410 390 123 4 247 632 7 392 0.0 533
MS.gene051219.t1 MTR_7g025740 67.008 391 121 4 244 630 3 389 0.0 531
MS.gene051219.t1 MTR_7g026110 66.667 390 121 5 244 629 3 387 0.0 528
MS.gene051219.t1 MTR_7g025790 67.692 390 118 4 244 629 3 388 0.0 526
MS.gene051219.t1 MTR_7g078190 65.985 391 124 4 244 629 3 389 3.03e-180 525
MS.gene051219.t1 MTR_7g451380 66.237 388 123 4 247 630 6 389 4.52e-180 524
MS.gene051219.t1 MTR_7g024030 66.923 390 121 4 244 629 3 388 1.06e-179 523
MS.gene051219.t1 MTR_8g103930 65.570 395 107 5 251 633 12 389 3.39e-179 522
MS.gene051219.t1 MTR_7g025720 66.667 387 121 4 244 626 3 385 1.28e-178 521
MS.gene051219.t1 MTR_7g026090 66.154 390 124 4 244 629 3 388 1.79e-178 520
MS.gene051219.t1 MTR_7g026170 66.237 388 123 4 247 630 6 389 4.65e-178 519
MS.gene051219.t1 MTR_7g029415 65.473 391 127 4 247 633 13 399 1.97e-177 518
MS.gene051219.t1 MTR_2g032160 65.957 376 120 4 245 616 4 375 2.83e-177 511
MS.gene051219.t1 MTR_7g025620 64.962 391 129 4 244 630 3 389 2.28e-176 515
MS.gene051219.t1 MTR_2g032580 64.796 392 130 4 245 632 4 391 4.19e-176 513
MS.gene051219.t1 MTR_2g022050 67.013 385 119 4 250 630 9 389 2.17e-175 512
MS.gene051219.t1 MTR_7g026130 65.394 393 128 4 245 633 4 392 2.78e-175 515
MS.gene051219.t1 MTR_4g079590 64.615 390 130 4 245 630 4 389 6.86e-175 511
MS.gene051219.t1 MTR_6g033850 66.837 392 124 4 245 634 4 391 1.00e-174 513
MS.gene051219.t1 MTR_7g025770 64.962 391 129 5 247 633 6 392 3.27e-174 509
MS.gene051219.t1 MTR_6g033760 64.796 392 132 3 245 634 4 391 8.63e-173 506
MS.gene051219.t1 MTR_7g025630 65.729 391 116 5 244 632 3 377 9.02e-173 505
MS.gene051219.t1 MTR_7g078210 65.897 390 125 4 244 629 3 388 2.61e-172 505
MS.gene051219.t1 MTR_7g011590 62.755 392 130 4 244 633 3 380 5.00e-172 503
MS.gene051219.t1 MTR_7g026140 63.868 393 118 4 245 633 4 376 4.61e-171 501
MS.gene051219.t1 MTR_2g017730 64.962 391 125 5 250 632 8 394 6.29e-166 488
MS.gene051219.t1 MTR_7g451360 62.953 386 109 3 247 630 6 359 3.02e-165 484
MS.gene051219.t1 MTR_2g038270 62.657 399 131 6 245 630 4 397 4.84e-164 484
MS.gene051219.t1 MTR_7g074460 62.087 393 123 5 244 632 3 373 4.22e-160 473
MS.gene051219.t1 MTR_6g033955 63.753 389 130 5 251 632 10 394 5.07e-160 474
MS.gene051219.t1 MTR_4g027145 60.750 400 137 6 247 630 6 401 6.69e-160 473
MS.gene051219.t1 MTR_6g033870 62.660 391 134 5 250 632 10 396 1.39e-156 466
MS.gene051219.t1 MTR_6g033820 63.281 384 130 5 250 626 9 388 4.23e-156 465
MS.gene051219.t1 MTR_1g017130 58.205 390 117 5 245 630 4 351 6.30e-151 449
MS.gene051219.t1 MTR_4g063720 62.051 390 75 5 245 634 4 320 9.74e-151 448
MS.gene051219.t1 MTR_8g099945 59.845 386 148 6 248 632 36 415 5.25e-150 449
MS.gene051219.t1 MTR_8g099795 60.104 386 147 6 248 632 36 415 3.36e-149 447
MS.gene051219.t1 MTR_7g025260 64.928 345 113 4 289 629 20 360 6.52e-149 444
MS.gene051219.t1 MTR_7g007000 57.506 393 115 5 245 633 4 348 4.20e-148 441
MS.gene051219.t1 MTR_1g041565 56.378 392 110 7 246 633 1 335 1.83e-142 425
MS.gene051219.t1 MTR_7g035155 64.669 317 104 4 247 559 6 318 2.97e-139 418
MS.gene051219.t1 MTR_1g077230 67.254 284 85 4 244 523 3 282 4.24e-129 382
MS.gene051219.t1 MTR_6g033800 63.487 304 105 4 331 632 1 300 1.22e-119 368
MS.gene051219.t1 MTR_2g010020 46.766 402 191 10 239 632 44 430 1.06e-101 324
MS.gene051219.t1 MTR_4g088515 49.091 385 103 8 250 630 276 571 1.26e-101 322
MS.gene051219.t1 MTR_2g010020 46.766 402 191 10 239 632 45 431 1.32e-101 324
MS.gene051219.t1 MTR_7g026080 63.415 246 82 4 245 486 4 245 3.29e-101 309
MS.gene051219.t1 MTR_4g092920 46.269 402 194 10 239 632 41 428 1.11e-99 319
MS.gene051219.t1 MTR_6g033810 53.226 310 89 4 327 632 8 265 5.35e-94 291
MS.gene051219.t1 MTR_6g034005 53.055 311 140 5 321 630 2 307 1.97e-89 293
MS.gene051219.t1 MTR_2g005690 41.549 426 221 10 223 634 38 449 5.89e-88 289
MS.gene051219.t1 MTR_2g031670 48.852 305 109 6 256 549 37 305 4.39e-87 275
MS.gene051219.t1 MTR_2g031670 72.917 48 13 0 585 632 275 322 3.21e-13 71.6
MS.gene051219.t1 MTR_2g040230 61.864 236 86 1 1 232 775 1010 6.25e-85 287
MS.gene051219.t1 MTR_6g033855 47.078 308 75 5 327 632 8 229 1.01e-72 237
MS.gene051219.t1 MTR_5g097190 49.609 256 78 5 244 497 152 358 1.24e-69 230
MS.gene051219.t1 MTR_2g102180 34.987 383 241 5 251 630 4 381 1.61e-69 242
MS.gene051219.t1 MTR_4g092920 45.455 308 148 8 331 632 1 294 1.05e-68 233
MS.gene051219.t1 MTR_4g012050 35.492 386 241 5 251 633 4 384 1.27e-68 239
MS.gene051219.t1 MTR_1207s0010 64.246 179 59 4 347 524 1 175 1.27e-63 208
MS.gene051219.t1 MTR_7g026050 68.056 144 46 0 486 629 1 144 1.96e-60 206
MS.gene051219.t1 MTR_6g478100 50.463 216 87 4 277 490 10 207 4.76e-60 203
MS.gene051219.t1 MTR_3g051300 31.865 386 255 5 251 633 4 384 1.11e-58 211
MS.gene051219.t1 MTR_5g092570 32.637 383 250 4 251 630 4 381 6.99e-57 206
MS.gene051219.t1 MTR_6g033910 47.907 215 59 5 313 525 19 182 3.62e-47 164
MS.gene051219.t1 MTR_3g081170 29.250 400 257 8 252 631 28 421 2.59e-45 173
MS.gene051219.t1 MTR_4g014720 41.016 256 126 6 2 232 447 702 2.09e-44 169
MS.gene051219.t1 MTR_5g006180 31.472 394 226 13 251 627 30 396 4.85e-44 166
MS.gene051219.t1 MTR_3g020500 43.689 206 110 3 2 207 17 216 9.03e-44 157
MS.gene051219.t1 MTR_5g090110 31.738 397 220 14 251 627 29 394 8.94e-43 162
MS.gene051219.t1 MTR_3g020650 42.308 208 118 1 2 207 774 981 6.33e-42 163
MS.gene051219.t1 MTR_7g026180 60.305 131 50 2 317 447 23 151 6.50e-42 155
MS.gene051219.t1 MTR_3g020640 41.706 211 118 2 2 207 788 998 3.67e-41 161
MS.gene051219.t1 MTR_5g005890 38.428 229 88 4 313 540 5 181 2.50e-40 145
MS.gene051219.t1 MTR_4g115780 30.946 391 239 12 250 617 140 522 7.90e-40 155
MS.gene051219.t1 MTR_2g040355 42.857 203 30 4 429 631 2 118 4.92e-37 140
MS.gene051219.t1 MTR_4g107920 28.993 407 250 13 238 627 12 396 2.95e-35 140
MS.gene051219.t1 MTR_3g020470 39.904 208 117 1 7 206 779 986 4.42e-32 133
MS.gene051219.t1 MTR_6g033805 76.471 68 16 0 245 312 4 71 3.36e-30 114
MS.gene051219.t1 MTR_3g020490 38.191 199 116 1 15 206 785 983 5.29e-30 127
MS.gene051219.t1 MTR_4g027235 74.286 70 16 1 247 314 6 75 1.93e-29 111
MS.gene051219.t1 MTR_4g027085 74.286 70 16 1 247 314 6 75 1.93e-29 111
MS.gene051219.t1 MTR_3g020420 39.429 175 99 1 2 169 587 761 1.26e-27 119
MS.gene051219.t1 MTR_7g026190 50.495 101 41 2 522 622 2 93 2.69e-24 101
MS.gene051219.t1 MTR_5g005870 51.042 96 47 0 539 634 68 163 5.43e-22 99.4
MS.gene051219.t1 MTR_1g077240 70.833 72 21 0 563 634 1 72 1.26e-19 90.1
MS.gene051219.t1 MTR_7g021430 35.294 170 98 4 466 624 11 179 1.90e-17 81.6
MS.gene051219.t1 MTR_7g026095 60.317 63 24 1 327 389 189 250 2.44e-15 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051219.t1 AT5G02500 80.612 392 70 3 245 634 4 391 0.0 636
MS.gene051219.t1 AT5G02500 80.612 392 70 3 245 634 4 391 0.0 635
MS.gene051219.t1 AT3G12580 79.847 392 73 3 245 634 4 391 0.0 631
MS.gene051219.t1 AT1G56410 80.102 392 72 3 245 634 4 391 0.0 630
MS.gene051219.t1 AT3G09440 79.847 392 73 3 245 634 4 391 0.0 628
MS.gene051219.t1 AT3G09440 79.847 392 73 3 245 634 4 391 0.0 628
MS.gene051219.t1 AT3G09440 79.847 392 73 3 245 634 4 391 0.0 628
MS.gene051219.t1 AT3G09440 79.847 392 73 3 245 634 4 391 0.0 628
MS.gene051219.t1 AT5G02490 78.571 392 78 3 245 634 4 391 0.0 625
MS.gene051219.t1 AT1G16030 74.479 384 92 3 251 632 9 388 0.0 577
MS.gene051219.t1 AT1G09080 60.519 385 146 5 248 632 40 418 8.38e-155 461
MS.gene051219.t1 AT1G09080 60.519 385 146 5 248 632 50 428 1.49e-154 461
MS.gene051219.t1 AT5G28540 61.140 386 143 6 248 632 35 414 5.90e-153 457
MS.gene051219.t1 AT5G42020 60.881 386 144 6 248 632 35 414 2.20e-152 453
MS.gene051219.t1 AT5G42020 60.881 386 144 6 248 632 35 414 3.62e-152 454
MS.gene051219.t1 AT5G42020 60.881 386 144 6 248 632 40 419 6.78e-152 454
MS.gene051219.t1 AT4G37910 46.117 412 198 10 230 632 33 429 5.39e-101 322
MS.gene051219.t1 AT4G37910 46.117 412 198 10 230 632 33 429 5.39e-101 322
MS.gene051219.t1 AT5G09590 46.667 390 185 10 251 632 60 434 7.61e-95 306
MS.gene051219.t1 AT5G49910 43.128 422 207 10 230 634 53 458 3.67e-89 292
MS.gene051219.t1 AT4G24280 44.792 384 196 8 251 629 81 453 7.59e-88 289
MS.gene051219.t1 AT1G79920 35.492 386 241 5 251 633 4 384 1.56e-71 245
MS.gene051219.t1 AT1G79920 35.492 386 241 5 251 633 4 384 1.56e-71 245
MS.gene051219.t1 AT1G79920 35.492 386 241 5 251 633 4 384 1.56e-71 245
MS.gene051219.t1 AT1G79920 35.492 386 241 5 251 633 4 384 1.84e-71 245
MS.gene051219.t1 AT1G79930 35.233 386 242 5 251 633 4 384 5.26e-71 245
MS.gene051219.t1 AT1G79930 35.233 386 242 5 251 633 4 384 1.56e-70 244
MS.gene051219.t1 AT1G11660 33.333 384 247 5 251 630 4 382 1.96e-63 224
MS.gene051219.t1 AT1G11660 33.333 384 247 5 251 630 4 382 1.96e-63 224
MS.gene051219.t1 AT1G11660 33.333 384 247 5 251 630 4 382 1.96e-63 224
MS.gene051219.t1 AT1G11660 34.195 348 222 5 286 630 2 345 2.85e-55 200
MS.gene051219.t1 AT4G16660 27.930 401 270 7 247 631 47 444 4.82e-49 184
MS.gene051219.t1 AT4G16660 28.607 402 266 9 247 631 23 420 7.00e-49 184
MS.gene051219.t1 AT2G32120 31.683 404 238 12 238 627 21 400 5.15e-47 174
MS.gene051219.t1 AT2G32120 31.683 404 238 12 238 627 21 400 5.15e-47 174

Find 136 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAATGGCTTGATCTTTA+AGG 0.136597 2.3:-49195066 MS.gene051219:CDS
AATACTTCTATGACATTATC+AGG 0.232984 2.3:+49197186 None:intergenic
ATCAATTTCAATGGTGGTTT+GGG 0.251837 2.3:+49194866 None:intergenic
ACGAATCATCCTTACGCTTT+TGG 0.256941 2.3:-49197077 MS.gene051219:CDS
AATGCTGCAGCCATTGCTTA+TGG 0.283536 2.3:-49195179 MS.gene051219:CDS
TTCAATAGTTGTTGAACCTT+TGG 0.284307 2.3:+49194669 None:intergenic
GATCATATTCAGAGCATAAA+TGG 0.299508 2.3:-49196855 MS.gene051219:CDS
AGGCCAGAAACTATCTTAAA+AGG 0.308511 2.3:+49195443 None:intergenic
CAAATTTCTGTCCGAGTCTA+TGG 0.317462 2.3:-49196692 MS.gene051219:CDS
ACCAACTTTGTTATCTTCAT+AGG 0.320468 2.3:+49196717 None:intergenic
AGCTAAACATGGATCTCTTT+AGG 0.327745 2.3:-49194788 MS.gene051219:CDS
AATCAATTTCAATGGTGGTT+TGG 0.329298 2.3:+49194865 None:intergenic
GACAGTGTTGATAGGGTTCC+TGG 0.332630 2.3:+49195535 None:intergenic
AGCACACCACAGAGCTATCT+TGG 0.348042 2.3:+49197038 None:intergenic
TTTAGTACTTCAATTCTTGA+TGG 0.354740 2.3:-49197165 MS.gene051219:CDS
CTATTGAAGGATTTCTTTAA+CGG 0.360458 2.3:-49194654 MS.gene051219:CDS
GACATTCTTCTTTCCAATGC+TGG 0.360564 2.3:+49195145 None:intergenic
CTTGAAATAATAATGACTTA+TGG 0.361508 2.3:-49197333 None:intergenic
AATTGGCTCTATAAGGATAA+TGG 0.369548 2.3:-49196791 MS.gene051219:CDS
TTCTTGAAGGGAGAGGAAAT+TGG 0.372746 2.3:-49197116 MS.gene051219:CDS
GCATCGAAGACAGTGTTGAT+AGG 0.378361 2.3:+49195527 None:intergenic
CTCAACCTCATAAGAGCTCT+TGG 0.378998 2.3:+49194930 None:intergenic
GATGTCTCTTTGCTAACTAC+TGG 0.397127 2.3:-49195092 MS.gene051219:CDS
GTTGCCGCCGCTGTTATTGC+TGG 0.402001 2.3:-49195233 MS.gene051219:CDS
ATAAGCAATGGCTGCAGCAT+TGG 0.404636 2.3:+49195181 None:intergenic
TTATAAACAAGCATGGCATC+TGG 0.406463 2.3:+49197255 None:intergenic
TGTTGTGGTCCTGTCATGGC+TGG 0.411375 2.3:+49196768 None:intergenic
AAGATAGAGGGACCGACGAT+TGG 0.415120 2.3:-49195713 MS.gene051219:CDS
TAAGCAATGGCTGCAGCATT+GGG 0.417899 2.3:+49195182 None:intergenic
AAGTTAACTATTCTCATCAA+TGG 0.422798 2.3:-49196973 MS.gene051219:CDS
CCATATGCAACAGCCTCATC+GGG 0.424062 2.3:+49194606 None:intergenic
AAAGGTTCAACAACTATTGA+AGG 0.425879 2.3:-49194667 MS.gene051219:CDS
TGGCTGCAGCATTGGGCTCC+AGG 0.429414 2.3:+49195189 None:intergenic
ACCTCCAAGATGGGTGTCTC+CGG 0.433425 2.3:+49195031 None:intergenic
ATAATGACTTATGGTTATAG+AGG 0.435648 2.3:-49197324 MS.gene051219:CDS
TTCTTGTTGTGGTCCTGTCA+TGG 0.437273 2.3:+49196764 None:intergenic
TTCGATGCTAAGAGGTTGAT+TGG 0.443804 2.3:-49195512 MS.gene051219:CDS
GAAGAGAAATAACATATAGT+TGG 0.444708 2.3:-49196651 MS.gene051219:CDS
TTGTAATTAACACCAATCAT+TGG 0.451428 2.3:+49195410 None:intergenic
ACCGACTCCGAGCGTCTCAT+TGG 0.451612 2.3:-49195581 MS.gene051219:CDS
CATACTCATGCGTAGGTGTA+TGG 0.452953 2.3:-49195673 MS.gene051219:CDS
GATAATGATTAAAGTAAGTA+GGG 0.456105 2.3:+49195273 None:intergenic
AGATAGAGGGACCGACGATT+GGG 0.461358 2.3:-49195712 MS.gene051219:CDS
ACGAAGGATGGGATTCTTGA+AGG 0.461898 2.3:-49197129 MS.gene051219:CDS
TCGTGTCAAGGGCATTATCC+TGG 0.462620 2.3:-49195207 MS.gene051219:CDS
TAAAATCTTCACCTCCAAGA+TGG 0.467266 2.3:+49195021 None:intergenic
CCCGATGAGGCTGTTGCATA+TGG 0.469856 2.3:-49194606 MS.gene051219:CDS
TGATAATGATTAAAGTAAGT+AGG 0.470114 2.3:+49195272 None:intergenic
GAGACGCTCGGAGTCGGTAA+AGG 0.470932 2.3:+49195586 None:intergenic
GTCCATGATGTTGTCCTTGT+AGG 0.484395 2.3:-49194708 MS.gene051219:CDS
CCTGGCAACTTGATTCTTAG+CGG 0.484748 2.3:+49195553 None:intergenic
AATGCTGATTTCACGAAGGA+TGG 0.484841 2.3:-49197141 MS.gene051219:CDS
AATGTCTTGATCTTTGACCT+TGG 0.489907 2.3:-49195128 MS.gene051219:CDS
TATTGCTGGTCTTCGTGTCA+AGG 0.490119 2.3:-49195219 MS.gene051219:CDS
TGAAATTGATTGTCTATTCG+AGG 0.490141 2.3:-49194853 MS.gene051219:CDS
ACCATATGCAACAGCCTCAT+CGG 0.495233 2.3:+49194605 None:intergenic
TCTGTCCGAGTCTATGGAAT+GGG 0.496062 2.3:-49196686 MS.gene051219:CDS
TTTAAGGTTAAAGCTACATC+CGG 0.496507 2.3:-49195050 MS.gene051219:CDS
ACGGTGTGGTTCTGTTACCT+TGG 0.496600 2.3:+49195618 None:intergenic
GTGTAAAGACAATATGTTGT+TGG 0.501333 2.3:-49197001 MS.gene051219:CDS
CTCTTCCCATTCCATAGACT+CGG 0.502205 2.3:+49196681 None:intergenic
CAAGAGCTCTTATGAGGTTG+AGG 0.502878 2.3:-49194929 MS.gene051219:CDS
GTCTTGATCTTTGACCTTGG+CGG 0.503421 2.3:-49195125 MS.gene051219:CDS
GCCTATGAAGATAACAAAGT+TGG 0.506383 2.3:-49196718 MS.gene051219:CDS
ACATCCGGAGACACCCATCT+TGG 0.507758 2.3:-49195035 MS.gene051219:CDS
CGAAGGATGGGATTCTTGAA+GGG 0.510134 2.3:-49197128 MS.gene051219:CDS
CGCCTACAAGGACAACATCA+TGG 0.514437 2.3:+49194706 None:intergenic
GGATAATGGCCAGCCATGAC+AGG 0.516702 2.3:-49196777 MS.gene051219:CDS
ATGTTTGAGAGATGCTAAGA+TGG 0.518945 2.3:-49194742 MS.gene051219:CDS
CCAACTTGGATTTCTTGTTG+TGG 0.519417 2.3:+49196753 None:intergenic
CATCGAAGACAGTGTTGATA+GGG 0.521853 2.3:+49195528 None:intergenic
GAATAGACAATCAATTTCAA+TGG 0.522605 2.3:+49194857 None:intergenic
CAATCATTGGCTTATCATCC+AGG 0.524677 2.3:+49195423 None:intergenic
CCGCTAAGAATCAAGTTGCC+AGG 0.524813 2.3:-49195553 MS.gene051219:CDS
TTGATCTTTGACCTTGGCGG+TGG 0.525604 2.3:-49195122 MS.gene051219:CDS
GAAATTGATTGTCTATTCGA+GGG 0.527246 2.3:-49194852 MS.gene051219:CDS
TCAACCTCATAAGAGCTCTT+GGG 0.527722 2.3:+49194931 None:intergenic
ACACTGTCTTCGATGCTAAG+AGG 0.528656 2.3:-49195520 MS.gene051219:CDS
GCATAAATGAAGGACCAACT+TGG 0.529926 2.3:+49196739 None:intergenic
ACCAATGAGACGCTCGGAGT+CGG 0.530619 2.3:+49195580 None:intergenic
GCAACCCAAGAGCTCTTATG+AGG 0.532545 2.3:-49194935 MS.gene051219:CDS
TTCAAATGTGTCACGAGTAA+CGG 0.533067 2.3:+49194812 None:intergenic
TAGAGGATTTCAATTGTTTG+CGG 0.534807 2.3:-49197307 MS.gene051219:CDS
GGTTCTGTTACCTTGGTCGT+TGG 0.535615 2.3:+49195625 None:intergenic
TCGGTAAAGGCAACGTAAGA+CGG 0.537315 2.3:+49195599 None:intergenic
TTATAGAGCCAATTGTCAAA+AGG 0.541239 2.3:+49196800 None:intergenic
TAGACAATCAATTTCAATGG+TGG 0.541724 2.3:+49194860 None:intergenic
TTCTTGCAGAACCGCCTACA+AGG 0.542199 2.3:+49194694 None:intergenic
TTCTGTCCGAGTCTATGGAA+TGG 0.542554 2.3:-49196687 MS.gene051219:CDS
ATTGCTGGTCTTCGTGTCAA+GGG 0.545822 2.3:-49195218 MS.gene051219:CDS
CTGGATGATAAGCCAATGAT+TGG 0.547812 2.3:-49195422 MS.gene051219:CDS
GAAGGATTTCTTTAACGGAA+AGG 0.548700 2.3:-49194649 MS.gene051219:CDS
AAAATCTTCACCTCCAAGAT+GGG 0.548945 2.3:+49195022 None:intergenic
GCACACCACAGAGCTATCTT+GGG 0.554491 2.3:+49197039 None:intergenic
CCGACGATTGGGATTGATCT+CGG 0.554996 2.3:-49195701 MS.gene051219:CDS
TCTTGAACTCTTGAACAAAG+TGG 0.555321 2.3:+49194982 None:intergenic
ATGCTGATTTCACGAAGGAT+GGG 0.565685 2.3:-49197140 MS.gene051219:CDS
TACACCTACGCATGAGTATG+TGG 0.568945 2.3:+49195676 None:intergenic
TCCGGAGACACCCATCTTGG+AGG 0.570454 2.3:-49195032 MS.gene051219:CDS
GATGGGATTCTTGAAGGGAG+AGG 0.572689 2.3:-49197123 MS.gene051219:CDS
CTTGTTAAAACCATAAGCAA+TGG 0.578447 2.3:+49195169 None:intergenic
CGGTTCTGCAAGAATTCCAA+AGG 0.582269 2.3:-49194685 MS.gene051219:CDS
ATCTTCATAGGCATAAATGA+AGG 0.582330 2.3:+49196729 None:intergenic
TTTAATGTCAGAGTCAAGGT+AGG 0.592849 2.3:+49195313 None:intergenic
GAAATCATCGCCAACGACCA+AGG 0.600490 2.3:-49195635 MS.gene051219:CDS
CTAACTACTGGAGAGAACAA+TGG 0.605558 2.3:-49195080 MS.gene051219:CDS
TTCAGAGAGATATGAAGTTG+TGG 0.606309 2.3:-49195466 MS.gene051219:CDS
CCACAACAAGAAATCCAAGT+TGG 0.610736 2.3:-49196753 MS.gene051219:CDS
CACATTTGAAGAGCTAAACA+TGG 0.610871 2.3:-49194799 MS.gene051219:CDS
AATGGAAGATATCTCTTCAG+CGG 0.613836 2.3:+49195367 None:intergenic
GGCATCACCAATGAGACGCT+CGG 0.614406 2.3:+49195574 None:intergenic
GTGTGATTTAGTGTGCAAAA+CGG 0.614666 2.3:-49196881 MS.gene051219:CDS
AACATATTGTCTTTACACAC+AGG 0.616489 2.3:+49197006 None:intergenic
ATGATTGGTGTTAATTACAA+CGG 0.623897 2.3:-49195407 MS.gene051219:CDS
ATACTTCTATGACATTATCA+GGG 0.625736 2.3:+49197187 None:intergenic
TTGCATCTTAATGAGTACAA+TGG 0.629345 2.3:+49195349 None:intergenic
CATGATGTTGTCCTTGTAGG+CGG 0.635301 2.3:-49194705 MS.gene051219:CDS
ATATTGTCTTTACACACAGG+AGG 0.638109 2.3:+49197009 None:intergenic
ATCAGGGCACATGTTAAGAG+AGG 0.641526 2.3:+49197203 None:intergenic
CATCAAAAGTACCACCGCCA+AGG 0.643146 2.3:+49195111 None:intergenic
GTTGAGGACTGCTTGTGAGA+AGG 0.649877 2.3:-49194913 MS.gene051219:CDS
CCGAGATCAATCCCAATCGT+CGG 0.655159 2.3:+49195701 None:intergenic
CGTTGGCGATGATTTCTACA+CGG 0.659400 2.3:+49195642 None:intergenic
AAAGGCAACGTAAGACGGTG+TGG 0.669222 2.3:+49195604 None:intergenic
AAATGTGTCACGAGTAACGG+TGG 0.669559 2.3:+49194815 None:intergenic
GGAACCACATACTCATGCGT+AGG 0.671107 2.3:-49195680 MS.gene051219:CDS
AACAGCGGCGGCAACCTCAG+TGG 0.675458 2.3:+49195241 None:intergenic
AAGACCAGCAATAACAGCGG+CGG 0.686599 2.3:+49195229 None:intergenic
AGTCTATGGAATGGGAAGAG+CGG 0.696006 2.3:-49196678 MS.gene051219:CDS
CTTCATATCTCTCTGAACAG+AGG 0.698908 2.3:+49195472 None:intergenic
TAAGATGCAAGAGATTGCTG+AGG 0.714197 2.3:-49195336 MS.gene051219:CDS
CATATGCAACAGCCTCATCG+GGG 0.745083 2.3:+49194607 None:intergenic
ACGAAGACCAGCAATAACAG+CGG 0.749307 2.3:+49195226 None:intergenic
TTATCAAATACAAGCCACTG+AGG 0.750248 2.3:-49195255 MS.gene051219:CDS
TGGTAATGCTGATTTCACGA+AGG 0.758486 2.3:-49197145 MS.gene051219:CDS
AATTGCCCAAGATAGCTCTG+TGG 0.769397 2.3:-49197044 MS.gene051219:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTTTTTTTTTATAA+AGG - chr2.3:49195982-49196001 MS.gene051219:intron 0.0%
!! TATTACTTAGAAGTTAAAAT+TGG + chr2.3:49195811-49195830 None:intergenic 15.0%
!!! AAATTTTATACTTTTTTCCA+AGG - chr2.3:49195655-49195674 MS.gene051219:CDS 15.0%
!! GATAATGATTAAAGTAAGTA+GGG + chr2.3:49196614-49196633 None:intergenic 20.0%
!! TAAGTATAAAATTTGATCCT+TGG + chr2.3:49195675-49195694 None:intergenic 20.0%
!! TTAATCCATTGAAAAATAAC+TGG - chr2.3:49195504-49195523 MS.gene051219:CDS 20.0%
!!! AAAACCTTAATTTTGCTTTA+TGG - chr2.3:49195848-49195867 MS.gene051219:intron 20.0%
!!! AAAATCCAGTTATTTTTCAA+TGG + chr2.3:49195512-49195531 None:intergenic 20.0%
!!! ATTTTCCTCTAACATTTTTA+GGG + chr2.3:49195403-49195422 None:intergenic 20.0%
!!! CAATGGATTAATTAAGTAAA+TGG + chr2.3:49195495-49195514 None:intergenic 20.0%
!!! TGAAGATTTTAATAACAGAA+TGG - chr2.3:49196875-49196894 MS.gene051219:CDS 20.0%
!!! TGATAATGATTAAAGTAAGT+AGG + chr2.3:49196615-49196634 None:intergenic 20.0%
!!! TTTAAATACCTTTTGACAAT+TGG - chr2.3:49195076-49195095 MS.gene051219:CDS 20.0%
!!! TTTTCCATAAAGCAAAATTA+AGG + chr2.3:49195855-49195874 None:intergenic 20.0%
! AAAAAAAGATAGAGTTCTCT+TGG + chr2.3:49196997-49197016 None:intergenic 25.0%
! AAAAACAAGAAGGATATTAG+TGG - chr2.3:49196927-49196946 MS.gene051219:CDS 25.0%
! AAGTTAACTATTCTCATCAA+TGG - chr2.3:49194911-49194930 MS.gene051219:CDS 25.0%
! AATACTTCTATGACATTATC+AGG + chr2.3:49194701-49194720 None:intergenic 25.0%
! ATACTTCTATGACATTATCA+GGG + chr2.3:49194700-49194719 None:intergenic 25.0%
! CTATTGAAGGATTTCTTTAA+CGG - chr2.3:49197230-49197249 MS.gene051219:CDS 25.0%
! GAAGAGAAATAACATATAGT+TGG - chr2.3:49195233-49195252 MS.gene051219:CDS 25.0%
! GAATAGACAATCAATTTCAA+TGG + chr2.3:49197030-49197049 None:intergenic 25.0%
! TGTTTATAAAAAAGGAAGTG+AGG - chr2.3:49194643-49194662 MS.gene051219:CDS 25.0%
! TTGTAATTAACACCAATCAT+TGG + chr2.3:49196477-49196496 None:intergenic 25.0%
! TTTAGTACTTCAATTCTTGA+TGG - chr2.3:49194719-49194738 MS.gene051219:CDS 25.0%
!! AAAATTTTCCGTTGGAATTT+TGG - chr2.3:49195375-49195394 MS.gene051219:CDS 25.0%
!! AAATTTTCCGTTGGAATTTT+GGG - chr2.3:49195376-49195395 MS.gene051219:CDS 25.0%
!! AATCTTGTAAAATTTTCCGT+TGG - chr2.3:49195367-49195386 MS.gene051219:CDS 25.0%
!! CATTTTCCTCTAACATTTTT+AGG + chr2.3:49195404-49195423 None:intergenic 25.0%
!! TATCTTTTCGATAAAAGGAT+AGG + chr2.3:49196157-49196176 None:intergenic 25.0%
!!! AAAAGTACAATTTTGGTTGT+AGG - chr2.3:49195436-49195455 MS.gene051219:CDS 25.0%
!!! ATGATTGGTGTTAATTACAA+CGG - chr2.3:49196477-49196496 MS.gene051219:intron 25.0%
!!! GAGGTTAAAAAGTACAATTT+TGG - chr2.3:49195429-49195448 MS.gene051219:CDS 25.0%
!!! TCCTTTTTTATAAACAAGCA+TGG + chr2.3:49194639-49194658 None:intergenic 25.0%
AAAGGTTCAACAACTATTGA+AGG - chr2.3:49197217-49197236 MS.gene051219:CDS 30.0%
AACATATTGTCTTTACACAC+AGG + chr2.3:49194881-49194900 None:intergenic 30.0%
AATCAATTTCAATGGTGGTT+TGG + chr2.3:49197022-49197041 None:intergenic 30.0%
ATCAATTTCAATGGTGGTTT+GGG + chr2.3:49197021-49197040 None:intergenic 30.0%
ATCTTCATAGGCATAAATGA+AGG + chr2.3:49195158-49195177 None:intergenic 30.0%
CTTGTTAAAACCATAAGCAA+TGG + chr2.3:49196718-49196737 None:intergenic 30.0%
GAAATTGATTGTCTATTCGA+GGG - chr2.3:49197032-49197051 MS.gene051219:CDS 30.0%
GATCATATTCAGAGCATAAA+TGG - chr2.3:49195029-49195048 MS.gene051219:CDS 30.0%
GCCATGCTTGTTTATAAAAA+AGG - chr2.3:49194635-49194654 MS.gene051219:CDS 30.0%
GTGTAAAGACAATATGTTGT+TGG - chr2.3:49194883-49194902 MS.gene051219:CDS 30.0%
GTTCAAGAGAAAAAACAAGA+AGG - chr2.3:49196917-49196936 MS.gene051219:CDS 30.0%
TAGACAATCAATTTCAATGG+TGG + chr2.3:49197027-49197046 None:intergenic 30.0%
TAGAGGATTTCAATTGTTTG+CGG - chr2.3:49194577-49194596 MS.gene051219:CDS 30.0%
TGAAATTGATTGTCTATTCG+AGG - chr2.3:49197031-49197050 MS.gene051219:CDS 30.0%
TTATAGAGCCAATTGTCAAA+AGG + chr2.3:49195087-49195106 None:intergenic 30.0%
TTCAATAGTTGTTGAACCTT+TGG + chr2.3:49197218-49197237 None:intergenic 30.0%
TTGCATCTTAATGAGTACAA+TGG + chr2.3:49196538-49196557 None:intergenic 30.0%
! ACCAACTTTGTTATCTTCAT+AGG + chr2.3:49195170-49195189 None:intergenic 30.0%
! CATTTTTAATGTCAGAGTCA+AGG + chr2.3:49196578-49196597 None:intergenic 30.0%
! CCTTTTATCGAAAAGATAGA+GGG - chr2.3:49196159-49196178 MS.gene051219:intron 30.0%
! GGATAAAGAAAAAGTGTGTT+CGG - chr2.3:49194598-49194617 MS.gene051219:CDS 30.0%
! TCCTTTTATCGAAAAGATAG+AGG - chr2.3:49196158-49196177 MS.gene051219:intron 30.0%
! TTTAAGGTTAAAGCTACATC+CGG - chr2.3:49196834-49196853 MS.gene051219:CDS 30.0%
!! AATTGGCTCTATAAGGATAA+TGG - chr2.3:49195093-49195112 MS.gene051219:CDS 30.0%
!!! ATTTTGAAACCAAAAGCGTA+AGG + chr2.3:49194819-49194838 None:intergenic 30.0%
!!! TTTTGACAATTGGCTCTATA+AGG - chr2.3:49195086-49195105 MS.gene051219:CDS 30.0%
AAAATCTTCACCTCCAAGAT+GGG + chr2.3:49196865-49196884 None:intergenic 35.0%
AAAGGATAGGAAGATCTGAA+AGG + chr2.3:49196144-49196163 None:intergenic 35.0%
AATGGAAGATATCTCTTCAG+CGG + chr2.3:49196520-49196539 None:intergenic 35.0%
AATGTCTTGATCTTTGACCT+TGG - chr2.3:49196756-49196775 MS.gene051219:CDS 35.0%
AGAAAAAGTTAACACTGCGT+TGG + chr2.3:49195786-49195805 None:intergenic 35.0%
AGCTAAACATGGATCTCTTT+AGG - chr2.3:49197096-49197115 MS.gene051219:CDS 35.0%
AGGCCAGAAACTATCTTAAA+AGG + chr2.3:49196444-49196463 None:intergenic 35.0%
ATATTGTCTTTACACACAGG+AGG + chr2.3:49194878-49194897 None:intergenic 35.0%
ATGTTTGAGAGATGCTAAGA+TGG - chr2.3:49197142-49197161 MS.gene051219:CDS 35.0%
CACATTTGAAGAGCTAAACA+TGG - chr2.3:49197085-49197104 MS.gene051219:CDS 35.0%
GAACAATGGCTTGATCTTTA+AGG - chr2.3:49196818-49196837 MS.gene051219:CDS 35.0%
GAAGGATTTCTTTAACGGAA+AGG - chr2.3:49197235-49197254 MS.gene051219:CDS 35.0%
GCCTATGAAGATAACAAAGT+TGG - chr2.3:49195166-49195185 MS.gene051219:CDS 35.0%
GTGTGATTTAGTGTGCAAAA+CGG - chr2.3:49195003-49195022 MS.gene051219:CDS 35.0%
GTGTGTATATATCAACGCAT+GGG - chr2.3:49195602-49195621 MS.gene051219:CDS 35.0%
GTTAGAGGAAAATGTTACAG+AGG - chr2.3:49195410-49195429 MS.gene051219:CDS 35.0%
TAAAATCTTCACCTCCAAGA+TGG + chr2.3:49196866-49196885 None:intergenic 35.0%
TCTTGAACTCTTGAACAAAG+TGG + chr2.3:49196905-49196924 None:intergenic 35.0%
TGTGTATATATCAACGCATG+GGG - chr2.3:49195603-49195622 MS.gene051219:CDS 35.0%
TGTGTGTATATATCAACGCA+TGG - chr2.3:49195601-49195620 MS.gene051219:CDS 35.0%
TTATAAACAAGCATGGCATC+TGG + chr2.3:49194632-49194651 None:intergenic 35.0%
TTATCAAATACAAGCCACTG+AGG - chr2.3:49196629-49196648 MS.gene051219:intron 35.0%
TTCAAATGTGTCACGAGTAA+CGG + chr2.3:49197075-49197094 None:intergenic 35.0%
TTCAGAGAGATATGAAGTTG+TGG - chr2.3:49196418-49196437 MS.gene051219:intron 35.0%
TTTAATGTCAGAGTCAAGGT+AGG + chr2.3:49196574-49196593 None:intergenic 35.0%
! CCCTCTATCTTTTCGATAAA+AGG + chr2.3:49196162-49196181 None:intergenic 35.0%
!!! TATAGTTGGTTTTGTCGTGT+CGG - chr2.3:49195247-49195266 MS.gene051219:CDS 35.0%
!!! TGGCCTTTTAAGATAGTTTC+TGG - chr2.3:49196438-49196457 MS.gene051219:intron 35.0%
!!! TTTTAAGATAGTTTCTGGCC+TGG - chr2.3:49196443-49196462 MS.gene051219:intron 35.0%
CAAAAAGATTAACCCCGATG+AGG - chr2.3:49197265-49197284 MS.gene051219:CDS 40.0%
CAAATTTCTGTCCGAGTCTA+TGG - chr2.3:49195192-49195211 MS.gene051219:CDS 40.0%
CCAACTTGGATTTCTTGTTG+TGG + chr2.3:49195134-49195153 None:intergenic 40.0%
CCACAACAAGAAATCCAAGT+TGG - chr2.3:49195131-49195150 MS.gene051219:CDS 40.0%
CTAACTACTGGAGAGAACAA+TGG - chr2.3:49196804-49196823 MS.gene051219:CDS 40.0%
CTGGATGATAAGCCAATGAT+TGG - chr2.3:49196462-49196481 MS.gene051219:intron 40.0%
CTTCATATCTCTCTGAACAG+AGG + chr2.3:49196415-49196434 None:intergenic 40.0%
GACATTCTTCTTTCCAATGC+TGG + chr2.3:49196742-49196761 None:intergenic 40.0%
GATGTCTCTTTGCTAACTAC+TGG - chr2.3:49196792-49196811 MS.gene051219:CDS 40.0%
GCATAAATGAAGGACCAACT+TGG + chr2.3:49195148-49195167 None:intergenic 40.0%
TAAGATGCAAGAGATTGCTG+AGG - chr2.3:49196548-49196567 MS.gene051219:intron 40.0%
TCAACCTCATAAGAGCTCTT+GGG + chr2.3:49196956-49196975 None:intergenic 40.0%
TGGGTCCCTAAAAATGTTAG+AGG - chr2.3:49195395-49195414 MS.gene051219:CDS 40.0%
TTAGGGACCCAAAATTCCAA+CGG + chr2.3:49195386-49195405 None:intergenic 40.0%
TTCTTGAAGGGAGAGGAAAT+TGG - chr2.3:49194768-49194787 MS.gene051219:CDS 40.0%
! ACGAATCATCCTTACGCTTT+TGG - chr2.3:49194807-49194826 MS.gene051219:CDS 40.0%
! TTCGATGCTAAGAGGTTGAT+TGG - chr2.3:49196372-49196391 MS.gene051219:intron 40.0%
!! AATGCTGATTTCACGAAGGA+TGG - chr2.3:49194743-49194762 MS.gene051219:CDS 40.0%
!! ATGCTGATTTCACGAAGGAT+GGG - chr2.3:49194744-49194763 MS.gene051219:CDS 40.0%
!! CAATCATTGGCTTATCATCC+AGG + chr2.3:49196464-49196483 None:intergenic 40.0%
!! CATCGAAGACAGTGTTGATA+GGG + chr2.3:49196359-49196378 None:intergenic 40.0%
!! GGTTTTAACAAGACCAGCAT+TGG - chr2.3:49196726-49196745 MS.gene051219:CDS 40.0%
!! TGGTAATGCTGATTTCACGA+AGG - chr2.3:49194739-49194758 MS.gene051219:CDS 40.0%
AAATGTGTCACGAGTAACGG+TGG + chr2.3:49197072-49197091 None:intergenic 45.0%
AATGCTGCAGCCATTGCTTA+TGG - chr2.3:49196705-49196724 MS.gene051219:CDS 45.0%
ACACTGTCTTCGATGCTAAG+AGG - chr2.3:49196364-49196383 MS.gene051219:intron 45.0%
ACCATATGCAACAGCCTCAT+CGG + chr2.3:49197282-49197301 None:intergenic 45.0%
ACGAAGACCAGCAATAACAG+CGG + chr2.3:49196661-49196680 None:intergenic 45.0%
ACGAAGGATGGGATTCTTGA+AGG - chr2.3:49194755-49194774 MS.gene051219:CDS 45.0%
AGTCTATGGAATGGGAAGAG+CGG - chr2.3:49195206-49195225 MS.gene051219:CDS 45.0%
ATAAGCAATGGCTGCAGCAT+TGG + chr2.3:49196706-49196725 None:intergenic 45.0%
ATCAGGGCACATGTTAAGAG+AGG + chr2.3:49194684-49194703 None:intergenic 45.0%
CAAGAGCTCTTATGAGGTTG+AGG - chr2.3:49196955-49196974 MS.gene051219:CDS 45.0%
CATACTCATGCGTAGGTGTA+TGG - chr2.3:49196211-49196230 MS.gene051219:intron 45.0%
CATGATGTTGTCCTTGTAGG+CGG - chr2.3:49197179-49197198 MS.gene051219:CDS 45.0%
CCTGGCAACTTGATTCTTAG+CGG + chr2.3:49196334-49196353 None:intergenic 45.0%
CGAAGGATGGGATTCTTGAA+GGG - chr2.3:49194756-49194775 MS.gene051219:CDS 45.0%
CGGTTCTGCAAGAATTCCAA+AGG - chr2.3:49197199-49197218 MS.gene051219:CDS 45.0%
CGTTGGCGATGATTTCTACA+CGG + chr2.3:49196245-49196264 None:intergenic 45.0%
CTCAACCTCATAAGAGCTCT+TGG + chr2.3:49196957-49196976 None:intergenic 45.0%
CTCTTCCCATTCCATAGACT+CGG + chr2.3:49195206-49195225 None:intergenic 45.0%
GTATATATCAACGCATGGGG+AGG - chr2.3:49195606-49195625 MS.gene051219:CDS 45.0%
GTCCATGATGTTGTCCTTGT+AGG - chr2.3:49197176-49197195 MS.gene051219:CDS 45.0%
GTCTTGATCTTTGACCTTGG+CGG - chr2.3:49196759-49196778 MS.gene051219:CDS 45.0%
TAAGCAATGGCTGCAGCATT+GGG + chr2.3:49196705-49196724 None:intergenic 45.0%
TACACCTACGCATGAGTATG+TGG + chr2.3:49196211-49196230 None:intergenic 45.0%
TCGGTAAAGGCAACGTAAGA+CGG + chr2.3:49196288-49196307 None:intergenic 45.0%
TCTGTCCGAGTCTATGGAAT+GGG - chr2.3:49195198-49195217 MS.gene051219:CDS 45.0%
TTCTGTCCGAGTCTATGGAA+TGG - chr2.3:49195197-49195216 MS.gene051219:CDS 45.0%
! AATTGCCCAAGATAGCTCTG+TGG - chr2.3:49194840-49194859 MS.gene051219:CDS 45.0%
! TTCTTGTTGTGGTCCTGTCA+TGG + chr2.3:49195123-49195142 None:intergenic 45.0%
!! ATTGCTGGTCTTCGTGTCAA+GGG - chr2.3:49196666-49196685 MS.gene051219:CDS 45.0%
!! GCATCGAAGACAGTGTTGAT+AGG + chr2.3:49196360-49196379 None:intergenic 45.0%
!! TATTGCTGGTCTTCGTGTCA+AGG - chr2.3:49196665-49196684 MS.gene051219:CDS 45.0%
!!! AAAAGTATAAAATTTTATAT+TGG + chr2.3:49195650-49195669 None:intergenic 5.0%
AAAGGCAACGTAAGACGGTG+TGG + chr2.3:49196283-49196302 None:intergenic 50.0%
AAGACCAGCAATAACAGCGG+CGG + chr2.3:49196658-49196677 None:intergenic 50.0%
AAGATAGAGGGACCGACGAT+TGG - chr2.3:49196171-49196190 MS.gene051219:intron 50.0%
AGATAGAGGGACCGACGATT+GGG - chr2.3:49196172-49196191 MS.gene051219:intron 50.0%
AGCACACCACAGAGCTATCT+TGG + chr2.3:49194849-49194868 None:intergenic 50.0%
CATATGCAACAGCCTCATCG+GGG + chr2.3:49197280-49197299 None:intergenic 50.0%
CCATATGCAACAGCCTCATC+GGG + chr2.3:49197281-49197300 None:intergenic 50.0%
CCGACGATTGGGATTGATCT+CGG - chr2.3:49196183-49196202 MS.gene051219:intron 50.0%
CCGAGATCAATCCCAATCGT+CGG + chr2.3:49196186-49196205 None:intergenic 50.0%
CGCCTACAAGGACAACATCA+TGG + chr2.3:49197181-49197200 None:intergenic 50.0%
GAAATCATCGCCAACGACCA+AGG - chr2.3:49196249-49196268 MS.gene051219:intron 50.0%
GATGGGATTCTTGAAGGGAG+AGG - chr2.3:49194761-49194780 MS.gene051219:CDS 50.0%
GCAACCCAAGAGCTCTTATG+AGG - chr2.3:49196949-49196968 MS.gene051219:CDS 50.0%
GCACACCACAGAGCTATCTT+GGG + chr2.3:49194848-49194867 None:intergenic 50.0%
GGAACCACATACTCATGCGT+AGG - chr2.3:49196204-49196223 MS.gene051219:intron 50.0%
GGTTCTGTTACCTTGGTCGT+TGG + chr2.3:49196262-49196281 None:intergenic 50.0%
GTTGAGGACTGCTTGTGAGA+AGG - chr2.3:49196971-49196990 MS.gene051219:CDS 50.0%
TTCTTGCAGAACCGCCTACA+AGG + chr2.3:49197193-49197212 None:intergenic 50.0%
TTGATCTTTGACCTTGGCGG+TGG - chr2.3:49196762-49196781 MS.gene051219:CDS 50.0%
! ACGGTGTGGTTCTGTTACCT+TGG + chr2.3:49196269-49196288 None:intergenic 50.0%
! CCGCTAAGAATCAAGTTGCC+AGG - chr2.3:49196331-49196350 MS.gene051219:intron 50.0%
!! CATCAAAAGTACCACCGCCA+AGG + chr2.3:49196776-49196795 None:intergenic 50.0%
!! GACAGTGTTGATAGGGTTCC+TGG + chr2.3:49196352-49196371 None:intergenic 50.0%
!! TCGTGTCAAGGGCATTATCC+TGG - chr2.3:49196677-49196696 MS.gene051219:CDS 50.0%
!!! GTTGGTTTTGTCGTGTCGGA+AGG - chr2.3:49195251-49195270 MS.gene051219:CDS 50.0%
ACATCCGGAGACACCCATCT+TGG - chr2.3:49196849-49196868 MS.gene051219:CDS 55.0%
ACCAATGAGACGCTCGGAGT+CGG + chr2.3:49196307-49196326 None:intergenic 55.0%
ACCTCCAAGATGGGTGTCTC+CGG + chr2.3:49196856-49196875 None:intergenic 55.0%
CCCGATGAGGCTGTTGCATA+TGG - chr2.3:49197278-49197297 MS.gene051219:CDS 55.0%
GGCATCACCAATGAGACGCT+CGG + chr2.3:49196313-49196332 None:intergenic 55.0%
TGTTGTGGTCCTGTCATGGC+TGG + chr2.3:49195119-49195138 None:intergenic 55.0%
! GGATAATGGCCAGCCATGAC+AGG - chr2.3:49195107-49195126 MS.gene051219:CDS 55.0%
GAGACGCTCGGAGTCGGTAA+AGG + chr2.3:49196301-49196320 None:intergenic 60.0%
GTTGCCGCCGCTGTTATTGC+TGG - chr2.3:49196651-49196670 MS.gene051219:CDS 60.0%
TCCGGAGACACCCATCTTGG+AGG - chr2.3:49196852-49196871 MS.gene051219:CDS 60.0%
! ACCGACTCCGAGCGTCTCAT+TGG - chr2.3:49196303-49196322 MS.gene051219:intron 60.0%
AACAGCGGCGGCAACCTCAG+TGG + chr2.3:49196646-49196665 None:intergenic 65.0%
! TGGCTGCAGCATTGGGCTCC+AGG + chr2.3:49196698-49196717 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 49194563 49197343 49194563 ID=MS.gene051219
chr2.3 mRNA 49194563 49197343 49194563 ID=MS.gene051219.t1;Parent=MS.gene051219
chr2.3 exon 49196634 49197343 49196634 ID=MS.gene051219.t1.exon1;Parent=MS.gene051219.t1
chr2.3 CDS 49196634 49197343 49196634 ID=cds.MS.gene051219.t1;Parent=MS.gene051219.t1
chr2.3 exon 49194563 49195757 49194563 ID=MS.gene051219.t1.exon2;Parent=MS.gene051219.t1
chr2.3 CDS 49194563 49195757 49194563 ID=cds.MS.gene051219.t1;Parent=MS.gene051219.t1
Gene Sequence

>MS.gene051219

ATGACTTATGGTTATAGAGGATTTCAATTGTTTGCGGATAAAGAAAAAGTGTGTTCGGAAGTGTTTCCAGATGCCATGCTTGTTTATAAAAAAGGAAGTGAGGAATTGCTTGATATGTCCTCTCTTAACATGTGCCCTGATAATGTCATAGAAGTATTTAGTACTTCAATTCTTGATGGTAATGCTGATTTCACGAAGGATGGGATTCTTGAAGGGAGAGGAAATTGGTATCGACATGAAAGCAACGAATCATCCTTACGCTTTTGGTTTCAAAATAAATTGCCCAAGATAGCTCTGTGGTGTGCTGTAGAACCTCCTGTGTGTAAAGACAATATGTTGTTGGATTTCAAGTTAACTATTCTCATCAATGGCACTAAGCAATTCACTTCTTCATGCAACTACATATTCTCTGCTGAAGAAAAAACTGAGCAAGTTCTTATGTGTGATTTAGTGTGCAAAACGGAAAGATCATATTCAGAGCATAAATGGAATCAAGTAGATATTTTATATGAGTTTAAATACCTTTTGACAATTGGCTCTATAAGGATAATGGCCAGCCATGACAGGACCACAACAAGAAATCCAAGTTGGTCCTTCATTTATGCCTATGAAGATAACAAAGTTGGTGTCAAATTTCTGTCCGAGTCTATGGAATGGGAAGAGCGGCACAGAAGAGAAATAACATATAGTTGGTTTTGTCGTGTCGGAAGGTTTGTTTGTGTATTGAGAAATGATTAATTAAGTAAATGATCACTATAAATATTAAGAATTTTGTTTAGTCTACACAAAAAAATTGATATTTTTAATCTTGTAAAATTTTCCGTTGGAATTTTGGGTCCCTAAAAATGTTAGAGGAAAATGTTACAGAGGTTAAAAAGTACAATTTTGGTTGTAGGAACTAAAAATGAATTTATGAATATTTATATAGACCATTTACTTAATTAATCCATTGAAAAATAACTGGATTTTGACATGCTTTAATTTATTTATTTTACTTTTTTGAAAACATTTGTAGAAGTATTGAAATCTACAAGAGTGTGTGTGTATATATCAACGCATGGGGAGGAATTATCTTAGAATTACACCAATATAAAATTTTATACTTTTTTCCAAGGATCAAATTTTATACTTAAAATCTAACATAATAATCACTCTTTTTTAAAACAAGCGTCACTGTAGCAAAAAATAGTTTGTTTCAAAATGAATGTAAGTTTAACTTTCCAACGCAGTGTTAACTTTTTCTTTCCAATTTTAACTTCTAAGTAATATAACGTACACTACAATCAAAACCTTAATTTTGCTTTATGGAAAACATAACAATTTTATTTTCTTTTCTAATCATTGTACTTCTTAATATGTGTGTAAAAACTTTAAAGACATTCATTTTGAAACTGATGCAGTATCATAATATGCATGTAATTTTAATTTTTTTTTTATAAAGGTAGAATATAATTTGTAAGTAGTTTGTTACACTTTTTTTTTTTTAAGATGTAGTTTATTATATCTAATATACAATAGTAGTTTATTATATCTAATATGCAATATAAGTAATATTACATAACATATTATATTTCACTTCCTTTCAGATCTTCCTATCCTTTTATCGAAAAGATAGAGGGACCGACGATTGGGATTGATCTCGGAACCACATACTCATGCGTAGGTGTATGGCAACATGACCGTGTAGAAATCATCGCCAACGACCAAGGTAACAGAACCACACCGTCTTACGTTGCCTTTACCGACTCCGAGCGTCTCATTGGTGATGCCGCTAAGAATCAAGTTGCCAGGAACCCTATCAACACTGTCTTCGATGCTAAGAGGTTGATTGGTCGTAGATTCAGCGATGCCTCTGTTCAGAGAGATATGAAGTTGTGGCCTTTTAAGATAGTTTCTGGCCTGGATGATAAGCCAATGATTGGTGTTAATTACAACGGTGAAGATAAGCAATTTTCCGCTGAAGAGATATCTTCCATTGTACTCATTAAGATGCAAGAGATTGCTGAGGCCTACCTTGACTCTGACATTAAAAATGTTGTTATCACTGTCCCTACTTACTTTAATCATTATCAAATACAAGCCACTGAGGTTGCCGCCGCTGTTATTGCTGGTCTTCGTGTCAAGGGCATTATCCTGGAGCCCAATGCTGCAGCCATTGCTTATGGTTTTAACAAGACCAGCATTGGAAAGAAGAATGTCTTGATCTTTGACCTTGGCGGTGGTACTTTTGATGTCTCTTTGCTAACTACTGGAGAGAACAATGGCTTGATCTTTAAGGTTAAAGCTACATCCGGAGACACCCATCTTGGAGGTGAAGATTTTAATAACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGAAAAAACAAGAAGGATATTAGTGGCAACCCAAGAGCTCTTATGAGGTTGAGGACTGCTTGTGAGAAGGCCAAGAGAACTCTATCTTTTTTTGCCCAAACCACCATTGAAATTGATTGTCTATTCGAGGGAATTGATTTCTGCTCCACCGTTACTCGTGACACATTTGAAGAGCTAAACATGGATCTCTTTAGGAAATGTATGAAGCAAGTTGAGAAATGTTTGAGAGATGCTAAGATGGACAAAAAAACTGTCCATGATGTTGTCCTTGTAGGCGGTTCTGCAAGAATTCCAAAGGTTCAACAACTATTGAAGGATTTCTTTAACGGAAAGGAGCTATGCAAAAAGATTAACCCCGATGAGGCTGTTGCATATGGTGCTGCAGTTTGCGCTGCATACTTGAATAATGAGTACAATTAG
Protein sequence

>MS.gene051219.t1

MTYGYRGFQLFADKEKVCSEVFPDAMLVYKKGSEELLDMSSLNMCPDNVIEVFSTSILDGNADFTKDGILEGRGNWYRHESNESSLRFWFQNKLPKIALWCAVEPPVCKDNMLLDFKLTILINGTKQFTSSCNYIFSAEEKTEQVLMCDLVCKTERSYSEHKWNQVDILYEFKYLLTIGSIRIMASHDRTTTRNPSWSFIYAYEDNKVGVKFLSESMEWEERHRREITYSWFCRVGRSSYPFIEKIEGPTIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVARNPINTVFDAKRLIGRRFSDASVQRDMKLWPFKIVSGLDDKPMIGVNYNGEDKQFSAEEISSIVLIKMQEIAEAYLDSDIKNVVITVPTYFNHYQIQATEVAAAVIAGLRVKGIILEPNAAAIAYGFNKTSIGKKNVLIFDLGGGTFDVSLLTTGENNGLIFKVKATSGDTHLGGEDFNNRMVNHFVQEFKRKNKKDISGNPRALMRLRTACEKAKRTLSFFAQTTIEIDCLFEGIDFCSTVTRDTFEELNMDLFRKCMKQVEKCLRDAKMDKKTVHDVVLVGGSARIPKVQQLLKDFFNGKELCKKINPDEAVAYGAAVCAAYLNNEYN