AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051951

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051951.t1 MTR_2g099870 92.985 613 39 4 1 611 1 611 0.0 1147
MS.gene051951.t1 MTR_8g104280 84.108 409 45 2 237 625 342 750 0.0 727
MS.gene051951.t1 MTR_8g104280 71.642 268 58 5 1 253 1 265 5.37e-99 319
MS.gene051951.t1 MTR_1g100210 36.744 215 115 8 385 578 684 898 1.17e-33 138
MS.gene051951.t1 MTR_4g025460 54.867 113 36 3 503 615 38 135 1.88e-21 92.4
MS.gene051951.t1 MTR_4g025470 56.627 83 35 1 114 196 1520 1601 7.41e-17 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051951.t1 AT4G10760 74.074 378 92 2 229 606 308 679 0.0 588
MS.gene051951.t1 AT4G10760 49.780 227 89 5 1 208 1 221 5.70e-53 193
MS.gene051951.t1 AT4G09980 35.885 209 99 10 385 565 617 818 2.77e-29 124
MS.gene051951.t1 AT4G09980 35.404 161 71 9 385 519 617 770 7.50e-18 88.2

Find 152 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAGCTTGCTGGAGATTC+AGG 0.173970 2.2:-3209191 MS.gene051951:CDS
TGCAACCGATAGTATGAATT+TGG 0.207772 2.2:-3208943 MS.gene051951:CDS
TTCCACTTTATCTAGGAAAT+TGG 0.217318 2.2:-3209255 MS.gene051951:CDS
GGACTAACTTTCTCTGATAT+TGG 0.225682 2.2:+3209347 None:intergenic
TAATTAATAAGAAATCATTT+AGG 0.231898 2.2:-3208905 MS.gene051951:CDS
CGCTGCTGCCTTAGTGGTTT+GGG 0.242037 2.2:+3208540 None:intergenic
CTGGCTTTCGGCTTGTTTCC+CGG 0.250664 2.2:+3208076 None:intergenic
CAACTCCACCTTTCTTGTTC+CGG 0.271026 2.2:+3208020 None:intergenic
TGGAGAGATGATTTCGATTT+GGG 0.273839 2.2:+3209434 None:intergenic
TTGGAGAGATGATTTCGATT+TGG 0.288507 2.2:+3209433 None:intergenic
AACTCCACCTTTCTTGTTCC+GGG 0.291160 2.2:+3208021 None:intergenic
AGGACTGAGAGCAAGGTTTA+AGG 0.294608 2.2:-3207920 MS.gene051951:CDS
AGTTGATGTTGTGCAGTTTC+AGG 0.295711 2.2:+3209026 None:intergenic
CGATTTGGGAGGGTTGAAAT+AGG 0.298630 2.2:+3209448 None:intergenic
TGCAGCCAGCATCACCTTCC+AGG 0.298893 2.2:-3207876 MS.gene051951:CDS
ACATTGTCTTGTTGGGATAA+AGG 0.301011 2.2:-3208151 MS.gene051951:CDS
TCTTCATACTGACATTAGTT+TGG 0.301506 2.2:-3208676 MS.gene051951:CDS
TTATTCAGACTCCAAGTATA+AGG 0.302457 2.2:-3208842 MS.gene051951:CDS
AATTAATAAGAAATCATTTA+GGG 0.304246 2.2:-3208904 MS.gene051951:CDS
ATGGACATATTAGGTAAGTT+TGG 0.307068 2.2:-3208447 MS.gene051951:CDS
TCAATGTTTCTGTTTACTTC+AGG 0.308190 2.2:+3208123 None:intergenic
TTGATTCTTCCACTTTATCT+AGG 0.314013 2.2:-3209262 MS.gene051951:CDS
TCGCTGCTGCCTTAGTGGTT+TGG 0.325097 2.2:+3208539 None:intergenic
TCGAGAGAAGAAACAAAATT+AGG 0.331810 2.2:+3209533 None:intergenic
AACTTTAGAATGGACATATT+AGG 0.337914 2.2:-3208456 MS.gene051951:CDS
ATCATTGTTGGAGGCAAATC+AGG 0.343274 2.2:+3208579 None:intergenic
CTTCATACTGACATTAGTTT+GGG 0.344638 2.2:-3208675 MS.gene051951:CDS
AAGCTTATTACATGCTATAA+AGG 0.346913 2.2:+3208719 None:intergenic
GGAGGGTTGAAATAGGTTAG+AGG 0.347303 2.2:+3209455 None:intergenic
CATTGTCTTGTTGGGATAAA+GGG 0.350805 2.2:-3208150 MS.gene051951:CDS
AGGTAAGTTTGGAGTAATAA+TGG 0.355596 2.2:-3208436 MS.gene051951:CDS
TTTGTTGGTAAGTCACAATA+TGG 0.356763 2.2:+3208768 None:intergenic
ATGTTATTGTTTCTGAAGTC+CGG 0.366102 2.2:-3208095 MS.gene051951:CDS
GCTGGCTGCACCTCCACATC+TGG 0.374605 2.2:+3207889 None:intergenic
ATTCACATGGAGCTGCCTTA+TGG 0.374787 2.2:-3208396 MS.gene051951:CDS
CATACATCTCATCTGGCTTT+CGG 0.378981 2.2:+3208064 None:intergenic
TTTGTCAGTTGTTCGATCAA+TGG 0.384398 2.2:-3209324 MS.gene051951:CDS
TCAGGTCGCTGCTGCCTTAG+TGG 0.385213 2.2:+3208534 None:intergenic
TGCCTTAGTGGTTTGGGAGG+AGG 0.388533 2.2:+3208546 None:intergenic
ACGAGAATGCTTAGAACGTT+GGG 0.403991 2.2:-3208274 MS.gene051951:CDS
GCCGCATTTCAAGAGCTTGC+TGG 0.406908 2.2:-3209200 MS.gene051951:CDS
ACTGGGCGTGCGATGGAACT+TGG 0.415975 2.2:-3208297 MS.gene051951:CDS
CTCCAATTTCCTAGATAAAG+TGG 0.425057 2.2:+3209253 None:intergenic
AGATGAGATGTATGCTTTGT+TGG 0.426898 2.2:-3208055 MS.gene051951:CDS
ACCTTCCAGGGCTTCTGCTA+TGG 0.432012 2.2:-3207863 MS.gene051951:CDS
AAGAGAGAACTTTGAGAGAA+AGG 0.436417 2.2:+3209510 None:intergenic
CTTCAGCGAATAATTAGAAC+TGG 0.443807 2.2:-3208207 MS.gene051951:CDS
GCAAGGAACATTGTCTTGTT+GGG 0.448473 2.2:-3208158 MS.gene051951:CDS
TGTTCATATGTGACTTGTGT+TGG 0.449891 2.2:+3209582 None:intergenic
AGCAAGGAACATTGTCTTGT+TGG 0.450367 2.2:-3208159 MS.gene051951:CDS
GAAGCCCTGGAAGGTGATGC+TGG 0.450433 2.2:+3207871 None:intergenic
CGCATGCACAATACTCATGC+AGG 0.450816 2.2:-3207991 MS.gene051951:CDS
GTTCATATGTGACTTGTGTT+GGG 0.451417 2.2:+3209583 None:intergenic
TCATCTGCCATTGTTCCATA+AGG 0.458815 2.2:+3208381 None:intergenic
GTAATCAATTGAGTGGTGTA+AGG 0.460248 2.2:-3207954 MS.gene051951:CDS
TTGGAAGAGTTTGCTGTAAG+TGG 0.460670 2.2:-3209104 MS.gene051951:CDS
TGATATTCGTAACTTTAGAA+TGG 0.461970 2.2:-3208466 MS.gene051951:CDS
TGATAATGGTGCTGTCGAAT+TGG 0.462983 2.2:-3209123 MS.gene051951:CDS
ATGGTAATATGAATCAAAAT+CGG 0.465303 2.2:-3208974 MS.gene051951:CDS
AACAAAGCATACATCTCATC+TGG 0.470575 2.2:+3208057 None:intergenic
CTTAGTGGTTTGGGAGGAGG+AGG 0.471885 2.2:+3208549 None:intergenic
AATGTGTTGAGGAGATCATT+TGG 0.474261 2.2:-3208245 MS.gene051951:CDS
TATTCAGACTCCAAGTATAA+GGG 0.476845 2.2:-3208841 MS.gene051951:CDS
ATGTGTTGAGGAGATCATTT+GGG 0.482047 2.2:-3208244 MS.gene051951:CDS
GAAGAATCAATTTCCACAAA+AGG 0.485391 2.2:+3209275 None:intergenic
GTCGCTGCTCTACAAACTCA+TGG 0.485915 2.2:-3208339 MS.gene051951:CDS
GGAGGAGCACACATCATTGT+TGG 0.486546 2.2:+3208567 None:intergenic
AGAAACAAAATTAGGTATAA+CGG 0.491151 2.2:+3209541 None:intergenic
TGAAATGCGGCTTTGTCTTG+TGG 0.495338 2.2:+3209212 None:intergenic
TGCCGGCAAGTACGAAGAAA+AGG 0.495538 2.2:+3208645 None:intergenic
GCTGCTAAGTTCAAAACTAA+AGG 0.497853 2.2:-3208807 MS.gene051951:CDS
ATTGAGTGGTGTAAGGTTAG+TGG 0.499280 2.2:-3207947 MS.gene051951:CDS
CGAGATAGCTTTGAGGTCAA+TGG 0.501626 2.2:-3209150 MS.gene051951:CDS
CATGCAGGGTGGATGTCTCT+TGG 0.503152 2.2:-3207976 MS.gene051951:CDS
GAACTGGACGTACGGGCCAT+TGG 0.503546 2.2:-3208191 MS.gene051951:CDS
AGGGTTGAAATAGGTTAGAG+GGG 0.504459 2.2:+3209457 None:intergenic
GAAGATTGTCGACGCCGGAC+TGG 0.504746 2.2:-3208744 MS.gene051951:CDS
ACAGCAGCCTCCCTTATACT+TGG 0.506226 2.2:+3208831 None:intergenic
CCTTGCTGTGATTGAGCCAA+TGG 0.508971 2.2:+3208175 None:intergenic
CAGGCAATTCTCTCAATAGA+CGG 0.510857 2.2:+3209045 None:intergenic
AGGTCAATGGCAGATGATAA+TGG 0.515834 2.2:-3209137 MS.gene051951:CDS
ATGGGTCACTGGGCGTGCGA+TGG 0.523044 2.2:-3208304 MS.gene051951:CDS
TTGCAGTCGAGATAGCTTTG+AGG 0.526784 2.2:-3209157 MS.gene051951:CDS
GAGATGATTTCGATTTGGGA+GGG 0.527822 2.2:+3209438 None:intergenic
ATTTGTAGTGACAACAAAAG+AGG 0.528653 2.2:+3209370 None:intergenic
GACGAGAATGCTTAGAACGT+TGG 0.531111 2.2:-3208275 MS.gene051951:CDS
GGCTTTGTCTTGTGGTGTAA+AGG 0.531124 2.2:+3209220 None:intergenic
TATTGTTCTGAAGTGGAACT+AGG 0.531691 2.2:-3208510 MS.gene051951:CDS
TGTTATTGTTTCTGAAGTCC+GGG 0.532244 2.2:-3208094 MS.gene051951:CDS
ATTTACATGTGTTCATGTGC+CGG 0.533106 2.2:+3208628 None:intergenic
CTCCTCCTCCCAAACCACTA+AGG 0.533869 2.2:-3208548 MS.gene051951:CDS
TACCTCCATAGCAGAAGCCC+TGG 0.536388 2.2:+3207858 None:intergenic
AGGCAATTCTCTCAATAGAC+GGG 0.537064 2.2:+3209046 None:intergenic
AATAATTAGAACTGGACGTA+CGG 0.539894 2.2:-3208199 MS.gene051951:CDS
ACCTGAGTATTGTTCTGAAG+TGG 0.541843 2.2:-3208517 MS.gene051951:CDS
GTTGGAGGCAAATCAGGTGT+AGG 0.545838 2.2:+3208585 None:intergenic
AAGTGGCAAATCAAGAATCA+TGG 0.546831 2.2:-3209087 MS.gene051951:CDS
TTCCAGGGCTTCTGCTATGG+AGG 0.547985 2.2:-3207860 MS.gene051951:CDS
GGTGTAAGGTTAGTGGATGA+AGG 0.549511 2.2:-3207940 MS.gene051951:CDS
ATGTAAATATGTTCACTATG+AGG 0.549551 2.2:-3208613 MS.gene051951:CDS
GATAAGTCCCGGAACAAGAA+AGG 0.550130 2.2:-3208028 MS.gene051951:CDS
ATCCTCCAAATTCATACTAT+CGG 0.550759 2.2:+3208938 None:intergenic
ATAATTAGAACTGGACGTAC+GGG 0.552693 2.2:-3208198 MS.gene051951:CDS
GCAGCCAGCATCACCTTCCA+GGG 0.553453 2.2:-3207875 MS.gene051951:CDS
CTAGTGTTGCTGCTCAAACA+AGG 0.557349 2.2:-3207831 MS.gene051951:CDS
TCATTCATCATCAATGGAGA+TGG 0.558757 2.2:-3209648 None:intergenic
AACCGATAGTATGAATTTGG+AGG 0.560456 2.2:-3208940 MS.gene051951:CDS
GGGTTGAAATAGGTTAGAGG+GGG 0.564873 2.2:+3209458 None:intergenic
AGAGATGATTTCGATTTGGG+AGG 0.566839 2.2:+3209437 None:intergenic
TTGGAGGCAAATCAGGTGTA+GGG 0.575681 2.2:+3208586 None:intergenic
ATGGAGATGGAGACAGATGA+AGG 0.579020 2.2:-3209635 MS.gene051951:CDS
AGAATCAATGGCCACTGATG+TGG 0.579978 2.2:-3207791 MS.gene051951:CDS
AGATGTATGCTTTGTTGGAG+AGG 0.584633 2.2:-3208050 MS.gene051951:CDS
TGGATGAAGGACTGAGAGCA+AGG 0.584679 2.2:-3207927 MS.gene051951:CDS
GCTAAGTTCAAAACTAAAGG+TGG 0.585659 2.2:-3208804 MS.gene051951:CDS
GAGGGTTGAAATAGGTTAGA+GGG 0.586233 2.2:+3209456 None:intergenic
CAGCAGCATGAATTGAGTCT+CGG 0.589239 2.2:-3208999 MS.gene051951:CDS
AGCTCCATGTGAATGTCCCA+CGG 0.591825 2.2:+3208405 None:intergenic
ATGCTATAAAGGAACCAGTC+CGG 0.595501 2.2:+3208730 None:intergenic
GCTATCTCGACTGCAAGCGT+TGG 0.599582 2.2:+3209164 None:intergenic
GGAGCACACATCATTGTTGG+AGG 0.602292 2.2:+3208570 None:intergenic
TGCTGCCTTAGTGGTTTGGG+AGG 0.608733 2.2:+3208543 None:intergenic
CATGAATTGAGTCTCGGAGA+TGG 0.610889 2.2:-3208993 MS.gene051951:CDS
TCGCTGCTCTACAAACTCAT+GGG 0.610983 2.2:-3208338 MS.gene051951:CDS
CTAAAGGTGGTTCTCAGATG+AGG 0.614681 2.2:-3208791 MS.gene051951:CDS
TCCAGCAAGCTCTTGAAATG+CGG 0.615211 2.2:+3209199 None:intergenic
TTAGTTAGTGTCCACATCAG+TGG 0.623018 2.2:+3207780 None:intergenic
TTGTTGGAGAGGATAAGTCC+CGG 0.623233 2.2:-3208039 MS.gene051951:CDS
TAAGGCAGCTTATCCAGATG+TGG 0.624770 2.2:-3207902 MS.gene051951:CDS
TTGGGGATATAAATGTGTTG+AGG 0.628466 2.2:-3208256 MS.gene051951:CDS
TCCACCGTGGGACATTCACA+TGG 0.629581 2.2:-3208409 MS.gene051951:CDS
TCCACTTCAGAACAATACTC+AGG 0.633399 2.2:+3208516 None:intergenic
GGCAGCTTATCCAGATGTGG+AGG 0.633458 2.2:-3207899 MS.gene051951:CDS
GGAGCTGCCTTATGGAACAA+TGG 0.636211 2.2:-3208388 MS.gene051951:CDS
TCCATAGCAGAAGCCCTGGA+AGG 0.636883 2.2:+3207862 None:intergenic
ATATCACAGTTGATCCATTG+TGG 0.637153 2.2:+3208483 None:intergenic
AGTGGCCATTGATTCTGTGA+CGG 0.637213 2.2:+3207798 None:intergenic
ATTGATCGAACAACTGACAA+AGG 0.638482 2.2:+3209326 None:intergenic
GAGCTTGCTGGAGATTCAGG+AGG 0.643407 2.2:-3209188 MS.gene051951:CDS
TGGAACTAGGCGAACCACAA+TGG 0.651967 2.2:-3208497 MS.gene051951:CDS
CAAAAGAAGATTGTCGACGC+CGG 0.652896 2.2:-3208749 MS.gene051951:CDS
CGAGAATGCTTAGAACGTTG+GGG 0.654390 2.2:-3208273 MS.gene051951:CDS
TCTCTTGGTAATCAATTGAG+TGG 0.668916 2.2:-3207961 MS.gene051951:CDS
AATAATGGCTGATCCACCGT+GGG 0.670381 2.2:-3208421 MS.gene051951:CDS
AAGTCCCGGAACAAGAAAGG+TGG 0.671413 2.2:-3208025 MS.gene051951:CDS
AGCTTGCTGGAGATTCAGGA+GGG 0.676077 2.2:-3209187 MS.gene051951:CDS
GCATGCACAATACTCATGCA+GGG 0.676682 2.2:-3207990 MS.gene051951:CDS
TCAGACTCCAAGTATAAGGG+AGG 0.680031 2.2:-3208838 MS.gene051951:CDS
CCATTGGCTCAATCACAGCA+AGG 0.689265 2.2:-3208175 MS.gene051951:CDS
TAATAATGGCTGATCCACCG+TGG 0.701095 2.2:-3208422 MS.gene051951:CDS
TGCACAATACTCATGCAGGG+TGG 0.701355 2.2:-3207987 MS.gene051951:CDS
TCCATGTGAATGTCCCACGG+TGG 0.777302 2.2:+3208408 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATAAGAAATCATTTA+GGG - chr2.2:3208511-3208530 MS.gene051951:CDS 10.0%
!! TAATTAATAAGAAATCATTT+AGG - chr2.2:3208510-3208529 MS.gene051951:CDS 10.0%
!!! TTATTATTATAAGGTTTTTG+AGG + chr2.2:3208014-3208033 None:intergenic 15.0%
!! AGAAACAAAATTAGGTATAA+CGG + chr2.2:3207877-3207896 None:intergenic 20.0%
!! ATGGTAATATGAATCAAAAT+CGG - chr2.2:3208441-3208460 MS.gene051951:CDS 20.0%
!!! TCATGGTTTTGAATATAAAT+AGG - chr2.2:3208345-3208364 MS.gene051951:CDS 20.0%
!!! TTCGAGGTTTTATTATTATA+AGG + chr2.2:3208023-3208042 None:intergenic 20.0%
! AAGCTTATTACATGCTATAA+AGG + chr2.2:3208699-3208718 None:intergenic 25.0%
! ATGTAAATATGTTCACTATG+AGG - chr2.2:3208802-3208821 MS.gene051951:CDS 25.0%
!! TGATATTCGTAACTTTAGAA+TGG - chr2.2:3208949-3208968 MS.gene051951:CDS 25.0%
!!! AACTTTAGAATGGACATATT+AGG - chr2.2:3208959-3208978 MS.gene051951:CDS 25.0%
AATAATTAGAACTGGACGTA+CGG - chr2.2:3209216-3209235 MS.gene051951:CDS 30.0%
ATCCTCCAAATTCATACTAT+CGG + chr2.2:3208480-3208499 None:intergenic 30.0%
ATGGACATATTAGGTAAGTT+TGG - chr2.2:3208968-3208987 MS.gene051951:CDS 30.0%
ATTTGTAGTGACAACAAAAG+AGG + chr2.2:3208048-3208067 None:intergenic 30.0%
GAAGAATCAATTTCCACAAA+AGG + chr2.2:3208143-3208162 None:intergenic 30.0%
TATTCAGACTCCAAGTATAA+GGG - chr2.2:3208574-3208593 MS.gene051951:CDS 30.0%
TCGAGAGAAGAAACAAAATT+AGG + chr2.2:3207885-3207904 None:intergenic 30.0%
TCTTCATACTGACATTAGTT+TGG - chr2.2:3208739-3208758 MS.gene051951:CDS 30.0%
TTATTCAGACTCCAAGTATA+AGG - chr2.2:3208573-3208592 MS.gene051951:CDS 30.0%
TTCCACTTTATCTAGGAAAT+TGG - chr2.2:3208160-3208179 MS.gene051951:CDS 30.0%
TTGATTCTTCCACTTTATCT+AGG - chr2.2:3208153-3208172 MS.gene051951:CDS 30.0%
! AGGTAAGTTTGGAGTAATAA+TGG - chr2.2:3208979-3208998 MS.gene051951:CDS 30.0%
! CTTCATACTGACATTAGTTT+GGG - chr2.2:3208740-3208759 MS.gene051951:CDS 30.0%
! TCAATGTTTCTGTTTACTTC+AGG + chr2.2:3209295-3209314 None:intergenic 30.0%
! TTTGTTGGTAAGTCACAATA+TGG + chr2.2:3208650-3208669 None:intergenic 30.0%
!! ATGTTATTGTTTCTGAAGTC+CGG - chr2.2:3209320-3209339 MS.gene051951:CDS 30.0%
!! TCATGGGCTTATTTTTCTAT+GGG - chr2.2:3209093-3209112 MS.gene051951:CDS 30.0%
!! TTATTTTTCTATGGGTCACT+GGG - chr2.2:3209101-3209120 MS.gene051951:CDS 30.0%
AACAAAGCATACATCTCATC+TGG + chr2.2:3209361-3209380 None:intergenic 35.0%
AACCGATAGTATGAATTTGG+AGG - chr2.2:3208475-3208494 MS.gene051951:CDS 35.0%
AAGTGGCAAATCAAGAATCA+TGG - chr2.2:3208328-3208347 MS.gene051951:CDS 35.0%
AATGTGTTGAGGAGATCATT+TGG - chr2.2:3209170-3209189 MS.gene051951:CDS 35.0%
AGATGAGATGTATGCTTTGT+TGG - chr2.2:3209360-3209379 MS.gene051951:CDS 35.0%
ATAATTAGAACTGGACGTAC+GGG - chr2.2:3209217-3209236 MS.gene051951:CDS 35.0%
ATATCACAGTTGATCCATTG+TGG + chr2.2:3208935-3208954 None:intergenic 35.0%
ATGTGTTGAGGAGATCATTT+GGG - chr2.2:3209171-3209190 MS.gene051951:CDS 35.0%
ATTGATCGAACAACTGACAA+AGG + chr2.2:3208092-3208111 None:intergenic 35.0%
ATTTACATGTGTTCATGTGC+CGG + chr2.2:3208790-3208809 None:intergenic 35.0%
CTCCAATTTCCTAGATAAAG+TGG + chr2.2:3208165-3208184 None:intergenic 35.0%
CTTCAGCGAATAATTAGAAC+TGG - chr2.2:3209208-3209227 MS.gene051951:CDS 35.0%
GCAAAAATACGAAACTGCTA+AGG - chr2.2:3208538-3208557 MS.gene051951:CDS 35.0%
GCTAAGTTCAAAACTAAAGG+TGG - chr2.2:3208611-3208630 MS.gene051951:CDS 35.0%
GCTGCTAAGTTCAAAACTAA+AGG - chr2.2:3208608-3208627 MS.gene051951:CDS 35.0%
GGACTAACTTTCTCTGATAT+TGG + chr2.2:3208071-3208090 None:intergenic 35.0%
GTAATCAATTGAGTGGTGTA+AGG - chr2.2:3209461-3209480 MS.gene051951:CDS 35.0%
TGCAACCGATAGTATGAATT+TGG - chr2.2:3208472-3208491 MS.gene051951:CDS 35.0%
TGGAGAGATGATTTCGATTT+GGG + chr2.2:3207984-3208003 None:intergenic 35.0%
TTGGAGAGATGATTTCGATT+TGG + chr2.2:3207985-3208004 None:intergenic 35.0%
TTGGGGATATAAATGTGTTG+AGG - chr2.2:3209159-3209178 MS.gene051951:CDS 35.0%
TTTGTCAGTTGTTCGATCAA+TGG - chr2.2:3208091-3208110 MS.gene051951:CDS 35.0%
! AAGAGAGAACTTTGAGAGAA+AGG + chr2.2:3207908-3207927 None:intergenic 35.0%
! ACATTGTCTTGTTGGGATAA+AGG - chr2.2:3209264-3209283 MS.gene051951:CDS 35.0%
! ATTTTCCGTCACAGAATCAA+TGG - chr2.2:3209612-3209631 MS.gene051951:CDS 35.0%
! CATTGTCTTGTTGGGATAAA+GGG - chr2.2:3209265-3209284 MS.gene051951:CDS 35.0%
! GTTCATATGTGACTTGTGTT+GGG + chr2.2:3207835-3207854 None:intergenic 35.0%
! TCTCTTGGTAATCAATTGAG+TGG - chr2.2:3209454-3209473 MS.gene051951:CDS 35.0%
! TGTTCATATGTGACTTGTGT+TGG + chr2.2:3207836-3207855 None:intergenic 35.0%
! TTTAAGTCGACTACCTTTTG+TGG - chr2.2:3208127-3208146 MS.gene051951:CDS 35.0%
!! CTCATGGGCTTATTTTTCTA+TGG - chr2.2:3209092-3209111 MS.gene051951:CDS 35.0%
!! CTTATTTTTCTATGGGTCAC+TGG - chr2.2:3209100-3209119 MS.gene051951:CDS 35.0%
!! TATTGTTCTGAAGTGGAACT+AGG - chr2.2:3208905-3208924 MS.gene051951:CDS 35.0%
!! TGTTATTGTTTCTGAAGTCC+GGG - chr2.2:3209321-3209340 MS.gene051951:CDS 35.0%
!!! AAGGTTTTTGAGGTAGTTGT+TGG + chr2.2:3208004-3208023 None:intergenic 35.0%
ACGAGAATGCTTAGAACGTT+GGG - chr2.2:3209141-3209160 MS.gene051951:CDS 40.0%
AGCAAGGAACATTGTCTTGT+TGG - chr2.2:3209256-3209275 MS.gene051951:CDS 40.0%
AGGCAATTCTCTCAATAGAC+GGG + chr2.2:3208372-3208391 None:intergenic 40.0%
AGGGTTGAAATAGGTTAGAG+GGG + chr2.2:3207961-3207980 None:intergenic 40.0%
AGGTCAATGGCAGATGATAA+TGG - chr2.2:3208278-3208297 MS.gene051951:CDS 40.0%
ATCATTGTTGGAGGCAAATC+AGG + chr2.2:3208839-3208858 None:intergenic 40.0%
ATGCTATAAAGGAACCAGTC+CGG + chr2.2:3208688-3208707 None:intergenic 40.0%
ATTGAGTGGTGTAAGGTTAG+TGG - chr2.2:3209468-3209487 MS.gene051951:CDS 40.0%
CAGGCAATTCTCTCAATAGA+CGG + chr2.2:3208373-3208392 None:intergenic 40.0%
GAGGGTTGAAATAGGTTAGA+GGG + chr2.2:3207962-3207981 None:intergenic 40.0%
TCATCTGCCATTGTTCCATA+AGG + chr2.2:3209037-3209056 None:intergenic 40.0%
TCCACTTCAGAACAATACTC+AGG + chr2.2:3208902-3208921 None:intergenic 40.0%
TTAGTTAGTGTCCACATCAG+TGG + chr2.2:3209638-3209657 None:intergenic 40.0%
! AGATGTATGCTTTGTTGGAG+AGG - chr2.2:3209365-3209384 MS.gene051951:CDS 40.0%
! AGTTGATGTTGTGCAGTTTC+AGG + chr2.2:3208392-3208411 None:intergenic 40.0%
! CGTCGACAATCTTCTTTTGT+TGG + chr2.2:3208665-3208684 None:intergenic 40.0%
! GACAACAAAAGAGGTTTTCG+AGG + chr2.2:3208039-3208058 None:intergenic 40.0%
! GCAAGGAACATTGTCTTGTT+GGG - chr2.2:3209257-3209276 MS.gene051951:CDS 40.0%
!! ACCTGAGTATTGTTCTGAAG+TGG - chr2.2:3208898-3208917 MS.gene051951:CDS 40.0%
!! AGAGATGATTTCGATTTGGG+AGG + chr2.2:3207981-3208000 None:intergenic 40.0%
!! CATACATCTCATCTGGCTTT+CGG + chr2.2:3209354-3209373 None:intergenic 40.0%
!! GAGATGATTTCGATTTGGGA+GGG + chr2.2:3207980-3207999 None:intergenic 40.0%
!! TGATAATGGTGCTGTCGAAT+TGG - chr2.2:3208292-3208311 MS.gene051951:CDS 40.0%
!! TTGGAAGAGTTTGCTGTAAG+TGG - chr2.2:3208311-3208330 MS.gene051951:CDS 40.0%
AACTCCACCTTTCTTGTTCC+GGG + chr2.2:3209397-3209416 None:intergenic 45.0%
AATAATGGCTGATCCACCGT+GGG - chr2.2:3208994-3209013 MS.gene051951:CDS 45.0%
AGAATCAATGGCCACTGATG+TGG - chr2.2:3209624-3209643 MS.gene051951:CDS 45.0%
AGGACTGAGAGCAAGGTTTA+AGG - chr2.2:3209495-3209514 MS.gene051951:CDS 45.0%
ATGGAGATGGAGACAGATGA+AGG - chr2.2:3207780-3207799 MS.gene051951:CDS 45.0%
ATTCACATGGAGCTGCCTTA+TGG - chr2.2:3209019-3209038 MS.gene051951:CDS 45.0%
CAAAAGAAGATTGTCGACGC+CGG - chr2.2:3208666-3208685 MS.gene051951:CDS 45.0%
CAACTCCACCTTTCTTGTTC+CGG + chr2.2:3209398-3209417 None:intergenic 45.0%
CAGCAGCATGAATTGAGTCT+CGG - chr2.2:3208416-3208435 MS.gene051951:CDS 45.0%
CATGAATTGAGTCTCGGAGA+TGG - chr2.2:3208422-3208441 MS.gene051951:CDS 45.0%
CGAGAATGCTTAGAACGTTG+GGG - chr2.2:3209142-3209161 MS.gene051951:CDS 45.0%
CTAAAGGTGGTTCTCAGATG+AGG - chr2.2:3208624-3208643 MS.gene051951:CDS 45.0%
GACGAGAATGCTTAGAACGT+TGG - chr2.2:3209140-3209159 MS.gene051951:CDS 45.0%
GATAAGTCCCGGAACAAGAA+AGG - chr2.2:3209387-3209406 MS.gene051951:CDS 45.0%
GCATGCACAATACTCATGCA+GGG - chr2.2:3209425-3209444 MS.gene051951:CDS 45.0%
GGAGGGTTGAAATAGGTTAG+AGG + chr2.2:3207963-3207982 None:intergenic 45.0%
GGGTTGAAATAGGTTAGAGG+GGG + chr2.2:3207960-3207979 None:intergenic 45.0%
TAAGGCAGCTTATCCAGATG+TGG - chr2.2:3209513-3209532 MS.gene051951:CDS 45.0%
TAATAATGGCTGATCCACCG+TGG - chr2.2:3208993-3209012 MS.gene051951:CDS 45.0%
TCAGACTCCAAGTATAAGGG+AGG - chr2.2:3208577-3208596 MS.gene051951:CDS 45.0%
TCCAGCAAGCTCTTGAAATG+CGG + chr2.2:3208219-3208238 None:intergenic 45.0%
TCGCTGCTCTACAAACTCAT+GGG - chr2.2:3209077-3209096 MS.gene051951:CDS 45.0%
TTGGAGGCAAATCAGGTGTA+GGG + chr2.2:3208832-3208851 None:intergenic 45.0%
TTGTTGGAGAGGATAAGTCC+CGG - chr2.2:3209376-3209395 MS.gene051951:CDS 45.0%
! AAATAGGTTAGAGGGGGTGT+AGG + chr2.2:3207954-3207973 None:intergenic 45.0%
! CGATTTGGGAGGGTTGAAAT+AGG + chr2.2:3207970-3207989 None:intergenic 45.0%
! CTAGTGTTGCTGCTCAAACA+AGG - chr2.2:3209584-3209603 MS.gene051951:CDS 45.0%
! GGCTTTGTCTTGTGGTGTAA+AGG + chr2.2:3208198-3208217 None:intergenic 45.0%
! GGTGTAAGGTTAGTGGATGA+AGG - chr2.2:3209475-3209494 MS.gene051951:CDS 45.0%
! GTCCTTTTCTTCGTACTTGC+CGG - chr2.2:3208768-3208787 MS.gene051951:CDS 45.0%
! TGAAATGCGGCTTTGTCTTG+TGG + chr2.2:3208206-3208225 None:intergenic 45.0%
! TTGCAGTCGAGATAGCTTTG+AGG - chr2.2:3208258-3208277 MS.gene051951:CDS 45.0%
!! AGTGGCCATTGATTCTGTGA+CGG + chr2.2:3209620-3209639 None:intergenic 45.0%
!! CGAGATAGCTTTGAGGTCAA+TGG - chr2.2:3208265-3208284 MS.gene051951:CDS 45.0%
AAGTCCCGGAACAAGAAAGG+TGG - chr2.2:3209390-3209409 MS.gene051951:CDS 50.0%
ACAGCAGCCTCCCTTATACT+TGG + chr2.2:3208587-3208606 None:intergenic 50.0%
AGCTCCATGTGAATGTCCCA+CGG + chr2.2:3209013-3209032 None:intergenic 50.0%
CGCATGCACAATACTCATGC+AGG - chr2.2:3209424-3209443 MS.gene051951:CDS 50.0%
GGAGCACACATCATTGTTGG+AGG + chr2.2:3208848-3208867 None:intergenic 50.0%
GGAGCTGCCTTATGGAACAA+TGG - chr2.2:3209027-3209046 MS.gene051951:CDS 50.0%
GGGGTGTAGGAGAAAAAGGA+CGG + chr2.2:3207941-3207960 None:intergenic 50.0%
GTCGCTGCTCTACAAACTCA+TGG - chr2.2:3209076-3209095 MS.gene051951:CDS 50.0%
GTGTAGGAGAAAAAGGACGG+TGG + chr2.2:3207938-3207957 None:intergenic 50.0%
GTTGGAGGCAAATCAGGTGT+AGG + chr2.2:3208833-3208852 None:intergenic 50.0%
TGCACAATACTCATGCAGGG+TGG - chr2.2:3209428-3209447 MS.gene051951:CDS 50.0%
TGCCGGCAAGTACGAAGAAA+AGG + chr2.2:3208773-3208792 None:intergenic 50.0%
TGGAACTAGGCGAACCACAA+TGG - chr2.2:3208918-3208937 MS.gene051951:CDS 50.0%
TGGATGAAGGACTGAGAGCA+AGG - chr2.2:3209488-3209507 MS.gene051951:CDS 50.0%
! AGAGGGGGTGTAGGAGAAAA+AGG + chr2.2:3207945-3207964 None:intergenic 50.0%
! CCTTGCTGTGATTGAGCCAA+TGG + chr2.2:3209243-3209262 None:intergenic 50.0%
! GGAGGAGCACACATCATTGT+TGG + chr2.2:3208851-3208870 None:intergenic 50.0%
!! AGCTTGCTGGAGATTCAGGA+GGG - chr2.2:3208228-3208247 MS.gene051951:CDS 50.0%
!! CAAGAGCTTGCTGGAGATTC+AGG - chr2.2:3208224-3208243 MS.gene051951:CDS 50.0%
!! CCATTGGCTCAATCACAGCA+AGG - chr2.2:3209240-3209259 MS.gene051951:CDS 50.0%
ACCTTCCAGGGCTTCTGCTA+TGG - chr2.2:3209552-3209571 MS.gene051951:CDS 55.0%
CATGCAGGGTGGATGTCTCT+TGG - chr2.2:3209439-3209458 MS.gene051951:CDS 55.0%
CGCTGCTGCCTTAGTGGTTT+GGG + chr2.2:3208878-3208897 None:intergenic 55.0%
CTCCTCCTCCCAAACCACTA+AGG - chr2.2:3208867-3208886 MS.gene051951:CDS 55.0%
GCCGCATTTCAAGAGCTTGC+TGG - chr2.2:3208215-3208234 MS.gene051951:CDS 55.0%
GCTATCTCGACTGCAAGCGT+TGG + chr2.2:3208254-3208273 None:intergenic 55.0%
GGCAGCTTATCCAGATGTGG+AGG - chr2.2:3209516-3209535 MS.gene051951:CDS 55.0%
TACCTCCATAGCAGAAGCCC+TGG + chr2.2:3209560-3209579 None:intergenic 55.0%
TCCACCGTGGGACATTCACA+TGG - chr2.2:3209006-3209025 MS.gene051951:CDS 55.0%
TCCATAGCAGAAGCCCTGGA+AGG + chr2.2:3209556-3209575 None:intergenic 55.0%
TCCATGTGAATGTCCCACGG+TGG + chr2.2:3209010-3209029 None:intergenic 55.0%
TCGCTGCTGCCTTAGTGGTT+TGG + chr2.2:3208879-3208898 None:intergenic 55.0%
TTCCAGGGCTTCTGCTATGG+AGG - chr2.2:3209555-3209574 MS.gene051951:CDS 55.0%
! CTGGCTTTCGGCTTGTTTCC+CGG + chr2.2:3209342-3209361 None:intergenic 55.0%
! CTTAGTGGTTTGGGAGGAGG+AGG + chr2.2:3208869-3208888 None:intergenic 55.0%
! TGCCTTAGTGGTTTGGGAGG+AGG + chr2.2:3208872-3208891 None:intergenic 55.0%
! TGCTGCCTTAGTGGTTTGGG+AGG + chr2.2:3208875-3208894 None:intergenic 55.0%
!! GAGCTTGCTGGAGATTCAGG+AGG - chr2.2:3208227-3208246 MS.gene051951:CDS 55.0%
ACTGGGCGTGCGATGGAACT+TGG - chr2.2:3209118-3209137 MS.gene051951:CDS 60.0%
GAACTGGACGTACGGGCCAT+TGG - chr2.2:3209224-3209243 MS.gene051951:CDS 60.0%
GAAGATTGTCGACGCCGGAC+TGG - chr2.2:3208671-3208690 MS.gene051951:CDS 60.0%
GAAGCCCTGGAAGGTGATGC+TGG + chr2.2:3209547-3209566 None:intergenic 60.0%
GCAGCCAGCATCACCTTCCA+GGG - chr2.2:3209540-3209559 MS.gene051951:CDS 60.0%
TCAGGTCGCTGCTGCCTTAG+TGG + chr2.2:3208884-3208903 None:intergenic 60.0%
TGCAGCCAGCATCACCTTCC+AGG - chr2.2:3209539-3209558 MS.gene051951:CDS 60.0%
ATGGGTCACTGGGCGTGCGA+TGG - chr2.2:3209111-3209130 MS.gene051951:CDS 65.0%
GCTGGCTGCACCTCCACATC+TGG + chr2.2:3209529-3209548 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 3207780 3209657 3207780 ID=MS.gene051951
chr2.2 mRNA 3207780 3209657 3207780 ID=MS.gene051951.t1;Parent=MS.gene051951
chr2.2 exon 3207780 3209657 3207780 ID=MS.gene051951.t1.exon1;Parent=MS.gene051951.t1
chr2.2 CDS 3207780 3209657 3207780 ID=cds.MS.gene051951.t1;Parent=MS.gene051951.t1
Gene Sequence

>MS.gene051951

ATGGAGATGGAGACAGATGAAGGTATAAACAGTCTTAAAGCTCGCATAGAAACCCAACACAAGTCACATATGAACATGCTTTCTTCTGTACAATCCGTTATACCTAATTTTGTTTCTTCTCTCGACCTTTCTCTCAAAGTTCTCTCTTCTTTTAACCACCGTCCTTTTTCTCCTACACCCCCTCTAACCTATTTCAACCCTCCCAAATCGAAATCATCTCTCCAACAACTACCTCAAAAACCTTATAATAATAAAACCTCGAAAACCTCTTTTGTTGTCACTACAAATCCAATATCAGAGAAAGTTAGTCCTTTGTCAGTTGTTCGATCAATGGTTGCTGTTTGTCTTTTAAGTCGACTACCTTTTGTGGAAATTGATTCTTCCACTTTATCTAGGAAATTGGAGAATGATCAAACCTTTACACCACAAGACAAAGCCGCATTTCAAGAGCTTGCTGGAGATTCAGGAGGGCCAACGCTTGCAGTCGAGATAGCTTTGAGGTCAATGGCAGATGATAATGGTGCTGTCGAATTGGAAGAGTTTGCTGTAAGTGGCAAATCAAGAATCATGGTTTTGAATATAAATAGGACCCGTCTATTGAGAGAATTGCCTGAAACTGCACAACATCAACTGCTGCAGCAGCATGAATTGAGTCTCGGAGATGGTAATATGAATCAAAATCGGCAACAAATTGCAACCGATAGTATGAATTTGGAGGATGTTGATGCTTTAATTAATAAGAAATCATTTAGGGAGCTGCAAAAATACGAAACTGCTAAGGAGCTTTTAAAAATTATTCAGACTCCAAGTATAAGGGAGGCTGCTGTAGCTGCTAAGTTCAAAACTAAAGGTGGTTCTCAGATGAGGCCATATTGTGACTTACCAACAAAAGAAGATTGTCGACGCCGGACTGGTTCCTTTATAGCATGTAATAAGCTTCATTTTAGACGAATCATTGCTCTTCATACTGACATTAGTTTGGGAGACTGTCCTTTTCTTCGTACTTGCCGGCACATGAACACATGTAAATATGTTCACTATGAGGATGACCCTACACCTGATTTGCCTCCAACAATGATGTGTGCTCCTCCTCCTCCCAAACCACTAAGGCAGCAGCGACCTGAGTATTGTTCTGAAGTGGAACTAGGCGAACCACAATGGATCAACTGTGATATTCGTAACTTTAGAATGGACATATTAGGTAAGTTTGGAGTAATAATGGCTGATCCACCGTGGGACATTCACATGGAGCTGCCTTATGGAACAATGGCAGATGATGAAATGCGCACTCTTAATGTCGCTGCTCTACAAACTCATGGGCTTATTTTTCTATGGGTCACTGGGCGTGCGATGGAACTTGGACGAGAATGCTTAGAACGTTGGGGATATAAATGTGTTGAGGAGATCATTTGGGTGAAAACAAATCAACTTCAGCGAATAATTAGAACTGGACGTACGGGCCATTGGCTCAATCACAGCAAGGAACATTGTCTTGTTGGGATAAAGGGTAGTCCTGAAGTAAACAGAAACATTGACACTGATGTTATTGTTTCTGAAGTCCGGGAAACAAGCCGAAAGCCAGATGAGATGTATGCTTTGTTGGAGAGGATAAGTCCCGGAACAAGAAAGGTGGAGTTGTTTGCTCGCATGCACAATACTCATGCAGGGTGGATGTCTCTTGGTAATCAATTGAGTGGTGTAAGGTTAGTGGATGAAGGACTGAGAGCAAGGTTTAAGGCAGCTTATCCAGATGTGGAGGTGCAGCCAGCATCACCTTCCAGGGCTTCTGCTATGGAGGTAGACTCTAGTGTTGCTGCTCAAACAAGGAGTTCATTTTCCGTCACAGAATCAATGGCCACTGATGTGGACACTAACTAA
Protein sequence

>MS.gene051951.t1

MEMETDEGINSLKARIETQHKSHMNMLSSVQSVIPNFVSSLDLSLKVLSSFNHRPFSPTPPLTYFNPPKSKSSLQQLPQKPYNNKTSKTSFVVTTNPISEKVSPLSVVRSMVAVCLLSRLPFVEIDSSTLSRKLENDQTFTPQDKAAFQELAGDSGGPTLAVEIALRSMADDNGAVELEEFAVSGKSRIMVLNINRTRLLRELPETAQHQLLQQHELSLGDGNMNQNRQQIATDSMNLEDVDALINKKSFRELQKYETAKELLKIIQTPSIREAAVAAKFKTKGGSQMRPYCDLPTKEDCRRRTGSFIACNKLHFRRIIALHTDISLGDCPFLRTCRHMNTCKYVHYEDDPTPDLPPTMMCAPPPPKPLRQQRPEYCSEVELGEPQWINCDIRNFRMDILGKFGVIMADPPWDIHMELPYGTMADDEMRTLNVAALQTHGLIFLWVTGRAMELGRECLERWGYKCVEEIIWVKTNQLQRIIRTGRTGHWLNHSKEHCLVGIKGSPEVNRNIDTDVIVSEVRETSRKPDEMYALLERISPGTRKVELFARMHNTHAGWMSLGNQLSGVRLVDEGLRARFKAAYPDVEVQPASPSRASAMEVDSSVAAQTRSSFSVTESMATDVDTN