AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052295

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTGTGGTGCTTGCTTTC+AGG 0.151650 4.2:-33150400 MS.gene052295:intron
GTTTCTTCACTCTTCTATAA+TGG 0.287049 4.2:-33150426 MS.gene052295:CDS
ACTAGGTTGGATCTTCAAAT+TGG 0.295466 4.2:-33148145 MS.gene052295:intron
AATTTAATTGCTAAATAATC+TGG 0.300940 4.2:+33148331 None:intergenic
CATCAGGGTATGATGGTAAA+TGG 0.338953 4.2:-33148040 MS.gene052295:CDS
GCAGCTGGGGCATGTGGATA+TGG 0.360150 4.2:-33150492 None:intergenic
GGCATTGCAATTTCGTTTAG+TGG 0.362944 4.2:-33148066 MS.gene052295:CDS
AAAAGGTTCTTCCAGCTGAT+TGG 0.372823 4.2:-33148007 MS.gene052295:CDS
TCTTTAGCACTTGACTTTAG+TGG 0.377568 4.2:-33150468 MS.gene052295:CDS
TTTCGTTTAGTGGTTACATC+AGG 0.387420 4.2:-33148056 MS.gene052295:CDS
TTCTATAATGGAGCTGGTTG+TGG 0.406537 4.2:-33150414 MS.gene052295:CDS
TCACTCTTCTATAATGGAGC+TGG 0.434156 4.2:-33150420 MS.gene052295:CDS
CTGTAAAGGCTCTACTGCTT+AGG 0.481361 4.2:+33150065 None:intergenic
AGTGGTGGACACCTTGCAGC+TGG 0.482221 4.2:-33150450 MS.gene052295:CDS
GGTATGATGGTAAATGGCTA+TGG 0.487295 4.2:-33148034 MS.gene052295:CDS
AGTTTCTTGAGCAGAAATCA+TGG 0.495684 4.2:-33148122 MS.gene052295:CDS
GTGGTTACATCAGGGTATGA+TGG 0.498564 4.2:-33148047 MS.gene052295:CDS
GGGCAGTGTGGGACACAAGT+AGG 0.499790 4.2:-33148100 MS.gene052295:CDS
CAGTAGAGCCTTTACAGCTA+TGG 0.510486 4.2:-33150059 MS.gene052295:CDS
GACCATGTTTCATCATTACA+TGG 0.510819 4.2:+33147921 None:intergenic
GTTTCTTGAGCAGAAATCAT+GGG 0.515717 4.2:-33148121 MS.gene052295:CDS
AGTAGGTGTTGATGTAGCTC+AGG 0.516706 4.2:-33148281 MS.gene052295:intron
CAGAAATCATGGGGCAGTGT+GGG 0.519402 4.2:-33148111 MS.gene052295:CDS
CAAGCAATATGCACAAAAGA+AGG 0.520775 4.2:-33150139 MS.gene052295:CDS
TGCCAAGTTTCAAAATCTGT+TGG 0.529827 4.2:+33150020 None:intergenic
AAGATCAGTCAATACCACTT+TGG 0.552511 4.2:+33150114 None:intergenic
GGCAGTGTGGGACACAAGTA+GGG 0.554581 4.2:-33148099 MS.gene052295:CDS
TTATATGCAGCTGGGGCATG+TGG 0.560990 4.2:-33150498 None:intergenic
CACAAGTAGGGTTCCACAAG+GGG 0.563760 4.2:-33148087 MS.gene052295:CDS
TTAGCACTTGACTTTAGTGG+TGG 0.565026 4.2:-33150465 MS.gene052295:CDS
TTCGTTTAGTGGTTACATCA+GGG 0.571759 4.2:-33148055 MS.gene052295:CDS
GATTACAGAAATTGCACAGG+AGG 0.581799 4.2:-33147952 MS.gene052295:CDS
TCAGATTACAGAAATTGCAC+AGG 0.586054 4.2:-33147955 MS.gene052295:CDS
CGAAATTGCAATGCCCCTTG+TGG 0.586600 4.2:+33148074 None:intergenic
GTATGATGGTAAATGGCTAT+GGG 0.593985 4.2:-33148033 MS.gene052295:CDS
TTGTTGACATTGAATACAAG+AGG 0.607511 4.2:-33149997 MS.gene052295:intron
GTGAAGAAACACCAGCTGCA+AGG 0.611075 4.2:+33150439 None:intergenic
ACACAAGTAGGGTTCCACAA+GGG 0.618151 4.2:-33148088 MS.gene052295:CDS
GCAGAAATCATGGGGCAGTG+TGG 0.638652 4.2:-33148112 MS.gene052295:CDS
ATTACAGAAATTGCACAGGA+GGG 0.647308 4.2:-33147951 MS.gene052295:CDS
TTTCTTGAGCAGAAATCATG+GGG 0.654832 4.2:-33148120 MS.gene052295:CDS
GACACAAGTAGGGTTCCACA+AGG 0.655516 4.2:-33148089 MS.gene052295:CDS
CACAAAAGAAGGTACCAAAG+TGG 0.665562 4.2:-33150128 MS.gene052295:CDS
CACCATGTAATGATGAAACA+TGG 0.686342 4.2:-33147923 MS.gene052295:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAATATATTTAATT+TGG + chr4.2:33148181-33148200 None:intergenic 0.0%
!! TTAAAAAAATAATTAACCAT+TGG - chr4.2:33150215-33150234 MS.gene052295:intron 10.0%
!! TTTATTTATCAATTTGTATA+TGG - chr4.2:33149947-33149966 MS.gene052295:intron 10.0%
!! AATTTAATTGCTAAATAATC+TGG + chr4.2:33150077-33150096 None:intergenic 15.0%
!! TTTAATTCAATAAAAACACT+TGG + chr4.2:33150200-33150219 None:intergenic 15.0%
!!! AATTAAATTTTTGTATCAAG+GGG - chr4.2:33150088-33150107 MS.gene052295:CDS 15.0%
!!! ATTTTATAATGTCGTAAATT+TGG - chr4.2:33149891-33149910 MS.gene052295:intron 15.0%
!!! CAATTAAATTTTTGTATCAA+GGG - chr4.2:33150087-33150106 MS.gene052295:CDS 15.0%
!! AATATATATGTTGAAGACAA+TGG - chr4.2:33149423-33149442 MS.gene052295:intron 20.0%
!!! ATTAAATTTTTGTATCAAGG+GGG - chr4.2:33150089-33150108 MS.gene052295:CDS 20.0%
!!! ATTATATGAAATCTAAGACA+AGG - chr4.2:33148636-33148655 MS.gene052295:intron 20.0%
!!! GCAATTAAATTTTTGTATCA+AGG - chr4.2:33150086-33150105 MS.gene052295:CDS 20.0%
!!! TATTTTTTCATTCTCTTTTG+CGG + chr4.2:33149492-33149511 None:intergenic 20.0%
!!! TTCATATAATTTGTCTACAT+TGG + chr4.2:33148626-33148645 None:intergenic 20.0%
!!! TTTTAGATCTGGAAAAAATT+CGG - chr4.2:33149360-33149379 MS.gene052295:intron 20.0%
! AACATTCCAAAAACTCAAAA+TGG + chr4.2:33149252-33149271 None:intergenic 25.0%
! ACATTCCAAAAACTCAAAAT+GGG + chr4.2:33149251-33149270 None:intergenic 25.0%
! CTTATTAATTACTACTAGGT+TGG - chr4.2:33150247-33150266 MS.gene052295:intron 25.0%
! TAATGTCGTAAATTTGGAAA+TGG - chr4.2:33149897-33149916 MS.gene052295:intron 25.0%
! TAGCGCAATAATAATAGTAA+CGG - chr4.2:33149759-33149778 MS.gene052295:intron 25.0%
! TGAGTACAAAAAACAGAATT+TGG - chr4.2:33150022-33150041 MS.gene052295:CDS 25.0%
! TGCAATCAATAATTCAACAT+TGG + chr4.2:33149180-33149199 None:intergenic 25.0%
! TGTAATTGGATTAATGGTAA+AGG + chr4.2:33149061-33149080 None:intergenic 25.0%
! TTACGAAGGAACAAAATTTA+AGG - chr4.2:33148983-33149002 MS.gene052295:intron 25.0%
!! ATCTAAAATTCATGTTGAAC+TGG + chr4.2:33149348-33149367 None:intergenic 25.0%
!! TTCAACATGAATTTTAGATC+TGG - chr4.2:33149349-33149368 MS.gene052295:intron 25.0%
!!! AGTTGATGAGTATATGAAAT+GGG - chr4.2:33149016-33149035 MS.gene052295:intron 25.0%
!!! ATTTAATTTGGCTAAATGTC+AGG + chr4.2:33148169-33148188 None:intergenic 25.0%
!!! CTAGATTTATTTTTTCAGTG+AGG + chr4.2:33149620-33149639 None:intergenic 25.0%
!!! TAGTTGATGAGTATATGAAA+TGG - chr4.2:33149015-33149034 MS.gene052295:intron 25.0%
AAAAAAACCTTTCCCATGAT+AGG - chr4.2:33149568-33149587 MS.gene052295:intron 30.0%
AAATCAAGATCTTGTTACGA+AGG - chr4.2:33148969-33148988 MS.gene052295:intron 30.0%
ATATATGTTGAAGACAATGG+AGG - chr4.2:33149426-33149445 MS.gene052295:intron 30.0%
ATTAATGAGCAATTTGATGC+AGG - chr4.2:33148229-33148248 MS.gene052295:intron 30.0%
GAAAAAATTCGGTATTCATG+GGG - chr4.2:33149371-33149390 MS.gene052295:intron 30.0%
GAAGTAAAGCTACAAAAAAG+TGG + chr4.2:33148447-33148466 None:intergenic 30.0%
GGAAAAAATTCGGTATTCAT+GGG - chr4.2:33149370-33149389 MS.gene052295:intron 30.0%
GTGGCTTATTAATTACTACT+AGG - chr4.2:33150243-33150262 MS.gene052295:intron 30.0%
GTTTCTTCACTCTTCTATAA+TGG - chr4.2:33147979-33147998 MS.gene052295:CDS 30.0%
TATATAATTGTCTTAGGGTG+AGG + chr4.2:33149741-33149760 None:intergenic 30.0%
TGCGCTATATAATTGTCTTA+GGG + chr4.2:33149746-33149765 None:intergenic 30.0%
TGGAAAAAATTCGGTATTCA+TGG - chr4.2:33149369-33149388 MS.gene052295:intron 30.0%
TGTGTCTGTAATTGGATTAA+TGG + chr4.2:33149067-33149086 None:intergenic 30.0%
TTCATATACTCATCAACTAC+TGG + chr4.2:33149014-33149033 None:intergenic 30.0%
TTCATCTAACATCACATCAA+TGG + chr4.2:33149113-33149132 None:intergenic 30.0%
TTGCGCTATATAATTGTCTT+AGG + chr4.2:33149747-33149766 None:intergenic 30.0%
TTGTTGACATTGAATACAAG+AGG - chr4.2:33148408-33148427 MS.gene052295:intron 30.0%
! AAGAGAGTGTGAAAAATATC+TGG + chr4.2:33148764-33148783 None:intergenic 30.0%
! ATATGAAATCTAAGACAAGG+TGG - chr4.2:33148639-33148658 MS.gene052295:intron 30.0%
! CTTGATTTTTCTTCCAAATC+TGG + chr4.2:33148957-33148976 None:intergenic 30.0%
! GTTGATGAGTATATGAAATG+GGG - chr4.2:33149017-33149036 MS.gene052295:intron 30.0%
! TTTTGGACGGTTAGATTAAA+GGG + chr4.2:33149552-33149571 None:intergenic 30.0%
! TTTTTGGACGGTTAGATTAA+AGG + chr4.2:33149553-33149572 None:intergenic 30.0%
!! AAATGAACTAGAAAGTGCTA+TGG + chr4.2:33149280-33149299 None:intergenic 30.0%
!! CATTGGTGTTCTATTTTCAA+TGG + chr4.2:33148609-33148628 None:intergenic 30.0%
!! TAATTAACCATTGGTTTCTG+TGG - chr4.2:33150224-33150243 MS.gene052295:intron 30.0%
!!! AATGGAGGTTTTTTTATCGA+AGG - chr4.2:33149441-33149460 MS.gene052295:intron 30.0%
!!! CACTTGGTAATGATTTTTGT+TGG + chr4.2:33150184-33150203 None:intergenic 30.0%
!!! GGATAGTTGATTTTGACTAT+TGG - chr4.2:33148699-33148718 MS.gene052295:intron 30.0%
!!! TATCATGGGAAAGGTTTTTT+TGG + chr4.2:33149569-33149588 None:intergenic 30.0%
!!! TGCATTTTTTGGAGTTCAAT+TGG - chr4.2:33149225-33149244 MS.gene052295:intron 30.0%
!!! TTCTGTTTTTTGTACTCACA+TGG + chr4.2:33150020-33150039 None:intergenic 30.0%
AAAATCAACTATCCCATGGT+GGG + chr4.2:33148693-33148712 None:intergenic 35.0%
AAGATCAGTCAATACCACTT+TGG + chr4.2:33148294-33148313 None:intergenic 35.0%
AGTCAAAATCAACTATCCCA+TGG + chr4.2:33148697-33148716 None:intergenic 35.0%
AGTTTCTTGAGCAGAAATCA+TGG - chr4.2:33150283-33150302 MS.gene052295:intron 35.0%
ATTACAGAAATTGCACAGGA+GGG - chr4.2:33150454-33150473 MS.gene052295:CDS 35.0%
CAAGCAATATGCACAAAAGA+AGG - chr4.2:33148266-33148285 MS.gene052295:intron 35.0%
CACCATGTAATGATGAAACA+TGG - chr4.2:33150482-33150501 MS.gene052295:CDS 35.0%
CGAAGGAACAAAATTTAAGG+AGG - chr4.2:33148986-33149005 MS.gene052295:intron 35.0%
GACCATGTTTCATCATTACA+TGG + chr4.2:33150487-33150506 None:intergenic 35.0%
GCTTTCAATGTGTCTGTAAT+TGG + chr4.2:33149075-33149094 None:intergenic 35.0%
GTATGATGGTAAATGGCTAT+GGG - chr4.2:33150372-33150391 MS.gene052295:intron 35.0%
GTTTCTTGAGCAGAAATCAT+GGG - chr4.2:33150284-33150303 MS.gene052295:intron 35.0%
TAAATGGCTATGGGCAAAAA+AGG - chr4.2:33150381-33150400 MS.gene052295:intron 35.0%
TAATAAGCCACAGAAACCAA+TGG + chr4.2:33150234-33150253 None:intergenic 35.0%
TATATGAAATGGGGTGATGA+AGG - chr4.2:33149026-33149045 MS.gene052295:intron 35.0%
TCAGATTACAGAAATTGCAC+AGG - chr4.2:33150450-33150469 MS.gene052295:CDS 35.0%
TCTTTAGCACTTGACTTTAG+TGG - chr4.2:33147937-33147956 MS.gene052295:CDS 35.0%
TGCCAAGTTTCAAAATCTGT+TGG + chr4.2:33148388-33148407 None:intergenic 35.0%
TTCCAGCTGATTGGAAAAAT+GGG - chr4.2:33150407-33150426 MS.gene052295:CDS 35.0%
TTCGTTTAGTGGTTACATCA+GGG - chr4.2:33150350-33150369 MS.gene052295:intron 35.0%
TTTCGTTTAGTGGTTACATC+AGG - chr4.2:33150349-33150368 MS.gene052295:intron 35.0%
TTTCTTGAGCAGAAATCATG+GGG - chr4.2:33150285-33150304 MS.gene052295:intron 35.0%
TTTGGACGGTTAGATTAAAG+GGG + chr4.2:33149551-33149570 None:intergenic 35.0%
! ACTAGGTTGGATCTTCAAAT+TGG - chr4.2:33150260-33150279 MS.gene052295:intron 35.0%
! CTTCTTTTGTGCATATTGCT+TGG + chr4.2:33148268-33148287 None:intergenic 35.0%
! GGTGTTCTATTTTCAATGGT+GGG + chr4.2:33148605-33148624 None:intergenic 35.0%
! TAATCCCACGTGGATTTTAA+TGG - chr4.2:33149985-33150004 MS.gene052295:intron 35.0%
! TCTTGATATGCCAGCATTTT+TGG - chr4.2:33148537-33148556 MS.gene052295:intron 35.0%
! TGGTGTTCTATTTTCAATGG+TGG + chr4.2:33148606-33148625 None:intergenic 35.0%
! TTTTCAGTGAGGTTTAGCAT+GGG + chr4.2:33149609-33149628 None:intergenic 35.0%
!! TGTCGGTGTTATTAATCTTG+CGG - chr4.2:33148083-33148102 MS.gene052295:CDS 35.0%
!! TTCTATTTTCAATGGTGGGA+CGG + chr4.2:33148601-33148620 None:intergenic 35.0%
!! TTTTTCAGTGAGGTTTAGCA+TGG + chr4.2:33149610-33149629 None:intergenic 35.0%
!!! AGCCAACAGATTTTGAAACT+TGG - chr4.2:33148383-33148402 MS.gene052295:CDS 35.0%
!!! ATGGGAAAGGTTTTTTTGGA+CGG + chr4.2:33149565-33149584 None:intergenic 35.0%
!!! ATTGGCCCATTTTGAGTTTT+TGG - chr4.2:33149243-33149262 MS.gene052295:intron 35.0%
AAAAGGTTCTTCCAGCTGAT+TGG - chr4.2:33150398-33150417 MS.gene052295:intron 40.0%
ACTCCAAAAAATGCATACGC+TGG + chr4.2:33149220-33149239 None:intergenic 40.0%
AGAAAATTGGGGATGGACAA+AGG + chr4.2:33148855-33148874 None:intergenic 40.0%
AGGAAGAGGCAGATTTGAAA+CGG + chr4.2:33148835-33148854 None:intergenic 40.0%
ATCGAAGGAGAAGACAATAG+AGG - chr4.2:33149456-33149475 MS.gene052295:intron 40.0%
ATGCTGGCATATCAAGAATG+TGG + chr4.2:33148534-33148553 None:intergenic 40.0%
CAAAATCAACTATCCCATGG+TGG + chr4.2:33148694-33148713 None:intergenic 40.0%
CATCAGGGTATGATGGTAAA+TGG - chr4.2:33150365-33150384 MS.gene052295:intron 40.0%
CTTCCAGCTGATTGGAAAAA+TGG - chr4.2:33150406-33150425 MS.gene052295:CDS 40.0%
GAGAAGACAATAGAGGTTGA+AGG - chr4.2:33149463-33149482 MS.gene052295:intron 40.0%
GAGCACAAATCATCGTATTC+AGG - chr4.2:33148031-33148050 MS.gene052295:CDS 40.0%
GATTACAGAAATTGCACAGG+AGG - chr4.2:33150453-33150472 MS.gene052295:CDS 40.0%
GGCATTGCAATTTCGTTTAG+TGG - chr4.2:33150339-33150358 MS.gene052295:intron 40.0%
GGTATGATGGTAAATGGCTA+TGG - chr4.2:33150371-33150390 MS.gene052295:intron 40.0%
TCCAGCTGATTGGAAAAATG+GGG - chr4.2:33150408-33150427 MS.gene052295:CDS 40.0%
TGAATTCCTTGAAGGTTGCT+GGG + chr4.2:33148929-33148948 None:intergenic 40.0%
TGCACCATTAAAATCCACGT+GGG + chr4.2:33149992-33150011 None:intergenic 40.0%
TTAGCACTTGACTTTAGTGG+TGG - chr4.2:33147940-33147959 MS.gene052295:CDS 40.0%
! CTTTTGAGCCATAGCTGTAA+AGG + chr4.2:33148357-33148376 None:intergenic 40.0%
! GAACATTGATTAATCCCACG+TGG - chr4.2:33149975-33149994 MS.gene052295:intron 40.0%
! GGAGAGTTGTTTCCTATCAT+GGG + chr4.2:33149583-33149602 None:intergenic 40.0%
! GTTGCAGTCATTGTTGTTGT+CGG - chr4.2:33148066-33148085 MS.gene052295:CDS 40.0%
! GTTGTTTCCTATCATGGGAA+AGG + chr4.2:33149578-33149597 None:intergenic 40.0%
! TCACTCTTCTATAATGGAGC+TGG - chr4.2:33147985-33148004 MS.gene052295:CDS 40.0%
! TGGAGAGTTGTTTCCTATCA+TGG + chr4.2:33149584-33149603 None:intergenic 40.0%
! TTCTATAATGGAGCTGGTTG+TGG - chr4.2:33147991-33148010 MS.gene052295:CDS 40.0%
!! CACAAAAGAAGGTACCAAAG+TGG - chr4.2:33148277-33148296 MS.gene052295:intron 40.0%
!! GGGGAGTATTGATCTTAGTA+TGG + chr4.2:33149532-33149551 None:intergenic 40.0%
!!! AGACCAGCGTATGCATTTTT+TGG - chr4.2:33149214-33149233 MS.gene052295:intron 40.0%
!!! GATATGCCAGCATTTTTGGT+TGG - chr4.2:33148541-33148560 MS.gene052295:intron 40.0%
AATTCCTTGAAGGTTGCTGG+GGG + chr4.2:33148927-33148946 None:intergenic 45.0%
ACACAAGTAGGGTTCCACAA+GGG - chr4.2:33150317-33150336 MS.gene052295:intron 45.0%
ACGAGGACGGTGAGAAAATT+GGG + chr4.2:33148867-33148886 None:intergenic 45.0%
AGTGAGGTTTAGCATGGGAA+TGG + chr4.2:33149604-33149623 None:intergenic 45.0%
ATGGGGTTCAACAGAAGAGA+AGG - chr4.2:33149388-33149407 MS.gene052295:intron 45.0%
CAGAAGAGAAGGGTAATTGC+AGG - chr4.2:33149399-33149418 MS.gene052295:intron 45.0%
CAGACGCAATGCAAAGATTC+TGG + chr4.2:33148132-33148151 None:intergenic 45.0%
CAGTAGAGCCTTTACAGCTA+TGG - chr4.2:33148346-33148365 MS.gene052295:CDS 45.0%
CTGAATTCCTTGAAGGTTGC+TGG + chr4.2:33148930-33148949 None:intergenic 45.0%
CTGCACCATTAAAATCCACG+TGG + chr4.2:33149993-33150012 None:intergenic 45.0%
CTGTAAAGGCTCTACTGCTT+AGG + chr4.2:33148343-33148362 None:intergenic 45.0%
CTTAGGGTGAGGTTTAGCAT+GGG + chr4.2:33149730-33149749 None:intergenic 45.0%
GAATTCCTTGAAGGTTGCTG+GGG + chr4.2:33148928-33148947 None:intergenic 45.0%
GGAATTCAGCTCACCAGATT+TGG - chr4.2:33148941-33148960 MS.gene052295:intron 45.0%
GTGGTTACATCAGGGTATGA+TGG - chr4.2:33150358-33150377 MS.gene052295:intron 45.0%
TCTTAGGGTGAGGTTTAGCA+TGG + chr4.2:33149731-33149750 None:intergenic 45.0%
TGCGGACGCAATGCAATTAT+AGG - chr4.2:33148101-33148120 MS.gene052295:CDS 45.0%
TGGGGTTCAACAGAAGAGAA+GGG - chr4.2:33149389-33149408 MS.gene052295:intron 45.0%
TGGTGAGCTGAATTCCTTGA+AGG + chr4.2:33148937-33148956 None:intergenic 45.0%
! AAGAGGCAGATTTGAAACGG+TGG + chr4.2:33148832-33148851 None:intergenic 45.0%
! CACTGTCTACTAGCTTAGGT+GGG + chr4.2:33148575-33148594 None:intergenic 45.0%
! TACTAGCTTAGGTGGGAAGT+GGG + chr4.2:33148568-33148587 None:intergenic 45.0%
!! ACCCCATTTTTCCAATCAGC+TGG + chr4.2:33150412-33150431 None:intergenic 45.0%
!! AGTAGGTGTTGATGTAGCTC+AGG - chr4.2:33150124-33150143 MS.gene052295:CDS 45.0%
AGACAAGGTGGCATTTAGCC+TGG - chr4.2:33148651-33148670 MS.gene052295:intron 50.0%
AGGGAAGTAGTTTCCCACCA+TGG - chr4.2:33148677-33148696 MS.gene052295:intron 50.0%
CACAAGTAGGGTTCCACAAG+GGG - chr4.2:33150318-33150337 MS.gene052295:intron 50.0%
CAGAAATCATGGGGCAGTGT+GGG - chr4.2:33150294-33150313 MS.gene052295:intron 50.0%
CCACCTAAGCTAGTAGACAG+TGG - chr4.2:33148573-33148592 MS.gene052295:intron 50.0%
CGAAATTGCAATGCCCCTTG+TGG + chr4.2:33150334-33150353 None:intergenic 50.0%
CGAGGACGGTGAGAAAATTG+GGG + chr4.2:33148866-33148885 None:intergenic 50.0%
GACAAGGTGGCATTTAGCCT+GGG - chr4.2:33148652-33148671 MS.gene052295:intron 50.0%
GACACAAGTAGGGTTCCACA+AGG - chr4.2:33150316-33150335 MS.gene052295:intron 50.0%
GACGAGGACGGTGAGAAAAT+TGG + chr4.2:33148868-33148887 None:intergenic 50.0%
GACGGTGAGAAAATTGGGGA+TGG + chr4.2:33148862-33148881 None:intergenic 50.0%
GGAAACTACTTCCCTTTCCC+AGG + chr4.2:33148672-33148691 None:intergenic 50.0%
GGGAAGTAGTTTCCCACCAT+GGG - chr4.2:33148678-33148697 MS.gene052295:intron 50.0%
GGGGGTCAAACAGAGATAGT+AGG - chr4.2:33150107-33150126 MS.gene052295:CDS 50.0%
GGTGAGGTTTAGCATGGGAA+TGG + chr4.2:33149725-33149744 None:intergenic 50.0%
GGTGAGTGAAGAGTGGATGA+AGG + chr4.2:33148903-33148922 None:intergenic 50.0%
GTGAAGAAACACCAGCTGCA+AGG + chr4.2:33147969-33147988 None:intergenic 50.0%
GTGGCATTTAGCCTGGGAAA+GGG - chr4.2:33148658-33148677 MS.gene052295:intron 50.0%
GTGGGTCCAACCAAAAATGC+TGG + chr4.2:33148550-33148569 None:intergenic 50.0%
TGGTTGTGGTGCTTGCTTTC+AGG - chr4.2:33148005-33148024 MS.gene052295:CDS 50.0%
TTGGGGATGGACAAAGGAAG+AGG + chr4.2:33148849-33148868 None:intergenic 50.0%
! AGGCAGATTTGAAACGGTGG+TGG + chr4.2:33148829-33148848 None:intergenic 50.0%
! CCACTGTCTACTAGCTTAGG+TGG + chr4.2:33148576-33148595 None:intergenic 50.0%
! CTACTAGCTTAGGTGGGAAG+TGG + chr4.2:33148569-33148588 None:intergenic 50.0%
! GTGCCACTGTCTACTAGCTT+AGG + chr4.2:33148579-33148598 None:intergenic 50.0%
!! AGAGTGGATGAAGGCGTTCA+AGG + chr4.2:33148894-33148913 None:intergenic 50.0%
GCAGAAATCATGGGGCAGTG+TGG - chr4.2:33150293-33150312 MS.gene052295:intron 55.0%
GGCAGTGTGGGACACAAGTA+GGG - chr4.2:33150306-33150325 MS.gene052295:intron 55.0%
GGTGGCATTTAGCCTGGGAA+AGG - chr4.2:33148657-33148676 MS.gene052295:intron 55.0%
! TGGTGGACACAGAGGAGAGT+GGG + chr4.2:33148812-33148831 None:intergenic 55.0%
!! TGAAACGGTGGTGGACACAG+AGG + chr4.2:33148820-33148839 None:intergenic 55.0%
!! TGGATGAAGGCGTTCAAGGC+AGG + chr4.2:33148890-33148909 None:intergenic 55.0%
ACAGAGGAGAGTGGGGATGG+AGG + chr4.2:33148804-33148823 None:intergenic 60.0%
ACCACCCCCAGCAACCTTCA+AGG - chr4.2:33148920-33148939 MS.gene052295:intron 60.0%
AGAGTGGGGATGGAGGAGAG+AGG + chr4.2:33148797-33148816 None:intergenic 60.0%
CGTTCAAGGCAGGACGAGGA+CGG + chr4.2:33148880-33148899 None:intergenic 60.0%
GACACAGAGGAGAGTGGGGA+TGG + chr4.2:33148807-33148826 None:intergenic 60.0%
GGATGGAGGAGAGAGGAGAG+TGG + chr4.2:33148790-33148809 None:intergenic 60.0%
GGGCAGTGTGGGACACAAGT+AGG - chr4.2:33150305-33150324 MS.gene052295:intron 60.0%
GGTGGACACAGAGGAGAGTG+GGG + chr4.2:33148811-33148830 None:intergenic 60.0%
TCCTTGAAGGTTGCTGGGGG+TGG + chr4.2:33148924-33148943 None:intergenic 60.0%
TGGGGGTGGTGAGTGAAGAG+TGG + chr4.2:33148910-33148929 None:intergenic 60.0%
! AAGGCGTTCAAGGCAGGACG+AGG + chr4.2:33148884-33148903 None:intergenic 60.0%
!! AGTGGTGGACACCTTGCAGC+TGG - chr4.2:33147955-33147974 MS.gene052295:CDS 60.0%
!! GTGGTGGACACAGAGGAGAG+TGG + chr4.2:33148813-33148832 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 33147917 33150510 33147917 ID=MS.gene052295
chr4.2 mRNA 33147917 33150510 33147917 ID=MS.gene052295.t1;Parent=MS.gene052295
chr4.2 exon 33150401 33150510 33150401 ID=MS.gene052295.t1.exon1;Parent=MS.gene052295.t1
chr4.2 CDS 33150401 33150510 33150401 ID=cds.MS.gene052295.t1;Parent=MS.gene052295.t1
chr4.2 exon 33149998 33150176 33149998 ID=MS.gene052295.t1.exon2;Parent=MS.gene052295.t1
chr4.2 CDS 33149998 33150176 33149998 ID=cds.MS.gene052295.t1;Parent=MS.gene052295.t1
chr4.2 exon 33148282 33148414 33148282 ID=MS.gene052295.t1.exon3;Parent=MS.gene052295.t1
chr4.2 CDS 33148282 33148414 33148282 ID=cds.MS.gene052295.t1;Parent=MS.gene052295.t1
chr4.2 exon 33147917 33148162 33147917 ID=MS.gene052295.t1.exon4;Parent=MS.gene052295.t1
chr4.2 CDS 33147917 33148162 33147917 ID=cds.MS.gene052295.t1;Parent=MS.gene052295.t1
Gene Sequence

>MS.gene052295

CTGGGGCATGTGGATATGGTTCTTTAGCACTTGACTTTAGTGGTGGACACCTTGCAGCTGGTGTTTCTTCACTCTTCTATAATGGAGCTGGTTGTGGTGCTTGCTTTCAGGTATGAGCACAAATCATCGTATTCAGGTTTTAAATTGCAGTTGCAGTCATTGTTGTTGTCGGTGTTATTAATCTTGCGGACGCAATGCAATTATAGGAGTCACCAGAATCTTTGCATTGCGTCTGCAATTTAAAACTATCCTGACATTTAGCCAAATTAAATATATTTTTTATTGATGTCATGAAAATTTTGTTTTTATGAAATTAATGAGCAATTTGATGCAGGTAAGATGCAAGAACCAAGCAATATGCACAAAAGAAGGTACCAAAGTGGTATTGACTGATCTTAATCACAATAATCAAACTGATTTTGTCCTAAGCAGTAGAGCCTTTACAGCTATGGCTCAAAAGAGTATGAGCCAACAGATTTTGAAACTTGGCATTGTTGACATTGAATACAAGAGGTAAGACATTTAAACCACTTTTTTGTAGCTTTACTTCAGTTATAAGCTGTTTCTAGATAAGTTCTAGATTTTCTTTTGTTAATGTAGCTTTTGTTTTTTTTCCACATTCTTGATATGCCAGCATTTTTGGTTGGACCCACTTCCCACCTAAGCTAGTAGACAGTGGCACCGTCCCACCATTGAAAATAGAACACCAATGTAGACAAATTATATGAAATCTAAGACAAGGTGGCATTTAGCCTGGGAAAGGGAAGTAGTTTCCCACCATGGGATAGTTGATTTTGACTATTGGATCAAATGAAGAACATGATATTATCATAGACTCTTAACACCAGATATTTTTCACACTCTCTTTTACCACTCTCCTCTCTCCTCCATCCCCACTCTCCTCTGTGTCCACCACCGTTTCAAATCTGCCTCTTCCTTTGTCCATCCCCAATTTTCTCACCGTCCTCGTCCTGCCTTGAACGCCTTCATCCACTCTTCACTCACCACCCCCAGCAACCTTCAAGGAATTCAGCTCACCAGATTTGGAAGAAAAATCAAGATCTTGTTACGAAGGAACAAAATTTAAGGAGGTTCCAGTAGTTGATGAGTATATGAAATGGGGTGATGAAGGAGCACATAGCCTTTACCATTAATCCAATTACAGACACATTGAAAGCTACTCATTTTAGAAACCATTGATGTGATGTTAGATGAACTTATGCTAAAAGTTGAACTAGTATTATTAGATCTGCATCTTCTCCAATGTTGAATTATTGATTGCAATATTTTATTTGCAAGACCAGCGTATGCATTTTTTGGAGTTCAATTGGCCCATTTTGAGTTTTTGGAATGTTCAACACCATAGCACTTTCTAGTTCATTTATTTATAATTGAAGAGTAATCAAATAATTTAAGATAATAAGATTTCCAGTTCAACATGAATTTTAGATCTGGAAAAAATTCGGTATTCATGGGGTTCAACAGAAGAGAAGGGTAATTGCAGGAAATATATATGTTGAAGACAATGGAGGTTTTTTTATCGAAGGAGAAGACAATAGAGGTTGAAGGTCACCGCAAAAGAGAATGAAAAAATAAATCTAGTGTGAACAGACCATACTAAGATCAATACTCCCCTTTAATCTAACCGTCCAAAAAAACCTTTCCCATGATAGGAAACAACTCTCCATTCCCATGCTAAACCTCACTGAAAAAATAAATCTAGTGTGAACAGACCATACTAAGATCAATACTCCCCTTTAATCTAACCGTCCAAAAAAACCTTTCCCATGATAGGAAACAACTCTCCATTCCCATGCTAAACCTCACCCTAAGACAATTATATAGCGCAATAATAATAGTAACGGTCTAATCTGATGTACTATATATTAATTTTAATGTAGTGCATAACAATGTAATATATGTATAACACGTATAATGTCAGAATTCAGATATTTTGTGTCTGTGCAATGATTAATTTTATAATGTCGTAAATTTGGAAATGGTGCACAAAATTCATATTCATATATTTATTTATTTATCAATTTGTATATGGTTGCTGAACATTGATTAATCCCACGTGGATTTTAATGGTGCAGAGTACCATGTGAGTACAAAAAACAGAATTTGGCTGTTCGTGTTGAAGAATCAAGCAAAAAACCAGATTATTTAGCAATTAAATTTTTGTATCAAGGGGGTCAAACAGAGATAGTAGGTGTTGATGTAGCTCAGGTATATATATCATATGTTACATTAATTAATATATGCCAACAAAAATCATTACCAAGTGTTTTTATTGAATTAAAAAAATAATTAACCATTGGTTTCTGTGGCTTATTAATTACTACTAGGTTGGATCTTCAAATTGGAGTTTCTTGAGCAGAAATCATGGGGCAGTGTGGGACACAAGTAGGGTTCCACAAGGGGCATTGCAATTTCGTTTAGTGGTTACATCAGGGTATGATGGTAAATGGCTATGGGCAAAAAAGGTTCTTCCAGCTGATTGGAAAAATGGGGTGATATATGATTCTGATATTCAGATTACAGAAATTGCACAGGAGGGTTGTTCACCATGTAATGATGAAACATGGTCATAA
Protein sequence

>MS.gene052295.t1

LGHVDMVLUHLTLVVDTLQLVFLHSSIMELVVVLAFRUDARTKQYAQKKVPKWYULILITIIKLILSUAVEPLQLWLKRVUANRFUNLALLTLNTREYHVSTKNRIWLFVLKNQAKNQIIUQLNFCIKGVKQRUUVLMULRLDLQIGVSUAEIMGQCGTQVGFHKGHCNFVUWLHQGMMVNGYGQKRFFQLIGKMGUYMILIFRLQKLHRRVVHHVMMKHGH