Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052334.t1 | XP_003607351.2 | 99.2 | 1179 | 9 | 0 | 1 | 1179 | 1 | 1179 | 0.00E+00 | 2381.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052334.t1 | F4JLM5 | 76.0 | 1192 | 269 | 5 | 1 | 1178 | 1 | 1189 | 0.0e+00 | 1853.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052334.t1 | G7JQP6 | 99.2 | 1179 | 9 | 0 | 1 | 1179 | 1 | 1179 | 0.0e+00 | 2381.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052334 | MS.gene28978 | 0.916537 | 1.58E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052334.t1 | MTR_4g076850 | 99.237 | 1179 | 9 | 0 | 1 | 1179 | 1 | 1179 | 0.0 | 2433 |
| MS.gene052334.t1 | MTR_2g436760 | 26.266 | 849 | 520 | 26 | 13 | 815 | 107 | 895 | 6.89e-74 | 267 |
| MS.gene052334.t1 | MTR_6g027620 | 23.621 | 580 | 388 | 15 | 340 | 904 | 327 | 866 | 2.22e-32 | 137 |
| MS.gene052334.t1 | MTR_6g027620 | 27.863 | 262 | 164 | 7 | 7 | 260 | 73 | 317 | 5.01e-15 | 80.9 |
| MS.gene052334.t1 | MTR_1g101620 | 23.661 | 579 | 368 | 19 | 340 | 865 | 368 | 925 | 6.06e-24 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052334.t1 | AT4G10320 | 75.943 | 1193 | 270 | 5 | 1 | 1179 | 1 | 1190 | 0.0 | 1887 |
| MS.gene052334.t1 | AT5G49030 | 26.020 | 907 | 544 | 24 | 15 | 858 | 122 | 964 | 1.12e-76 | 276 |
| MS.gene052334.t1 | AT5G49030 | 26.020 | 907 | 544 | 24 | 15 | 858 | 308 | 1150 | 3.36e-76 | 276 |
| MS.gene052334.t1 | AT5G49030 | 26.471 | 850 | 512 | 22 | 15 | 815 | 122 | 907 | 1.22e-75 | 271 |
| MS.gene052334.t1 | AT1G14610 | 21.796 | 913 | 563 | 28 | 7 | 860 | 137 | 957 | 1.85e-37 | 153 |
| MS.gene052334.t1 | AT5G16715 | 24.096 | 581 | 382 | 16 | 340 | 904 | 322 | 859 | 4.94e-30 | 129 |
| MS.gene052334.t1 | AT5G16715 | 26.459 | 257 | 172 | 7 | 7 | 260 | 70 | 312 | 9.67e-11 | 67.0 |
| MS.gene052334.t1 | AT3G23145 | 64.286 | 70 | 10 | 1 | 180 | 249 | 1 | 55 | 1.20e-21 | 93.2 |
| MS.gene052334.t1 | AT3G23145 | 62.500 | 48 | 18 | 0 | 330 | 377 | 69 | 116 | 4.22e-15 | 74.7 |
Find 260 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCCTTGCTGACATTAA+TGG | 0.144243 | 4.4:-33752627 | MS.gene052334:CDS |
| CAATTAGTCGAAGTAGATTT+TGG | 0.147587 | 4.4:-33758141 | MS.gene052334:CDS |
| GCAAAACTTGAAAAGCTTTC+AGG | 0.188115 | 4.4:-33758055 | MS.gene052334:CDS |
| GTGCTAGCTACTGCATTATT+TGG | 0.188796 | 4.4:-33755946 | MS.gene052334:CDS |
| ATGACGCTCACGAATGTTTC+GGG | 0.222682 | 4.4:+33752797 | None:intergenic |
| AACGAGATCGATAAGAAATT+AGG | 0.233796 | 4.4:-33764695 | MS.gene052334:CDS |
| GCCTCTTTGGGTTTATCCTT+TGG | 0.238893 | 4.4:+33762718 | None:intergenic |
| GACCTTCAAATAGGTAAAAT+TGG | 0.246024 | 4.4:+33762927 | None:intergenic |
| TTCAAGAAGGAAGGAGTTTA+TGG | 0.248985 | 4.4:-33755576 | MS.gene052334:CDS |
| TGACATTGCTTTGACTTCTT+TGG | 0.255340 | 4.4:+33751663 | None:intergenic |
| GCTATTAGCAACAGCCTCTT+TGG | 0.267127 | 4.4:+33762705 | None:intergenic |
| CCTCTTTGGGTTTATCCTTT+GGG | 0.273011 | 4.4:+33762719 | None:intergenic |
| ATGGCCGTTATTGATGATTA+TGG | 0.273943 | 4.4:-33755829 | MS.gene052334:CDS |
| TTTGAAGCGGTCTGATATTA+AGG | 0.275943 | 4.4:-33751584 | MS.gene052334:intron |
| TAAGAATGATTATAAAACTA+TGG | 0.281435 | 4.4:-33764543 | MS.gene052334:CDS |
| GGTAAGCATCCATTTCTTTC+CGG | 0.288571 | 4.4:+33755245 | None:intergenic |
| TGGGTTTATCCTTTGGGTTA+TGG | 0.292970 | 4.4:+33762725 | None:intergenic |
| GTCCATTGTGCCCCTGCTTT+TGG | 0.296251 | 4.4:-33762381 | MS.gene052334:CDS |
| GATTTAACTGACCTTCAAAT+AGG | 0.312154 | 4.4:+33762918 | None:intergenic |
| AGGGAAGATGTGCTTAAGTT+AGG | 0.312813 | 4.4:-33764662 | MS.gene052334:CDS |
| AATGTCACAGGAAAGTATTT+TGG | 0.315868 | 4.4:-33751647 | MS.gene052334:CDS |
| TGAGGTTTACTTTCAATCAT+TGG | 0.318333 | 4.4:-33750480 | MS.gene052334:CDS |
| TATTGTACCTTGACGAAATC+AGG | 0.324130 | 4.4:+33758809 | None:intergenic |
| CTTTGTTATAGGTGATGTTT+TGG | 0.331649 | 4.4:-33750842 | MS.gene052334:intron |
| TGAGGCTGGTCAGAATTATA+AGG | 0.332587 | 4.4:-33763583 | MS.gene052334:intron |
| GCACGACAGGAGAGTTTATA+AGG | 0.334110 | 4.4:+33755616 | None:intergenic |
| TAAGAGACTAGGAAAATCTA+TGG | 0.341281 | 4.4:-33751695 | MS.gene052334:CDS |
| GATGTGCTGGAGAAATTCTC+AGG | 0.348213 | 4.4:-33762607 | MS.gene052334:CDS |
| TCAGGTACGCAATAAGTATA+CGG | 0.351134 | 4.4:-33762788 | MS.gene052334:intron |
| TATGACGCTCACGAATGTTT+CGG | 0.352456 | 4.4:+33752796 | None:intergenic |
| ATGCAGTAGCTAGCACCATT+AGG | 0.353959 | 4.4:+33755953 | None:intergenic |
| TGCTTCAATGATATCCTTGT+CGG | 0.355467 | 4.4:+33760817 | None:intergenic |
| GATGTATTCGGGTTTGTCTT+TGG | 0.358350 | 4.4:+33764871 | None:intergenic |
| GGATGCTTACCGCCTTTATA+CGG | 0.365894 | 4.4:-33755233 | MS.gene052334:intron |
| AAGAAGCAATCCTCTCATTC+TGG | 0.369198 | 4.4:-33764931 | MS.gene052334:CDS |
| ACGAGATCGATAAGAAATTA+GGG | 0.374612 | 4.4:-33764694 | MS.gene052334:CDS |
| TTACGAGATATGTTGAAGAT+TGG | 0.380607 | 4.4:-33764604 | MS.gene052334:CDS |
| ATGTGCTTAAGTTAGGGATT+GGG | 0.383988 | 4.4:-33764655 | MS.gene052334:CDS |
| CTTTCCACTTGAAGTTCCCT+TGG | 0.390143 | 4.4:+33762654 | None:intergenic |
| CTATTAGCAACAGCCTCTTT+GGG | 0.391463 | 4.4:+33762706 | None:intergenic |
| GGGAAGATGTGCTTAAGTTA+GGG | 0.394236 | 4.4:-33764661 | MS.gene052334:CDS |
| TCATCCTCACTAATCCATAA+AGG | 0.395309 | 4.4:+33758106 | None:intergenic |
| TCAGCAAGGAAATCTTTATC+AGG | 0.398587 | 4.4:+33752636 | None:intergenic |
| TACGAGATATGTTGAAGATT+GGG | 0.399386 | 4.4:-33764603 | MS.gene052334:CDS |
| TTGGGATTGTCATGGTTTAC+CGG | 0.400584 | 4.4:-33764723 | MS.gene052334:CDS |
| GACGATGTCTTTGATAGTTC+CGG | 0.400651 | 4.4:+33764793 | None:intergenic |
| GGCTGGAAAATGCAAGAGAT+TGG | 0.401395 | 4.4:-33758165 | MS.gene052334:CDS |
| AGGATAAGCGCTTTCACAAT+TGG | 0.404438 | 4.4:-33758186 | MS.gene052334:CDS |
| GAACAACTGCAGGTTATCAT+TGG | 0.405017 | 4.4:-33748458 | MS.gene052334:intron |
| CCAGTCCTTCAGCTACAAAA+TGG | 0.406398 | 4.4:+33757076 | None:intergenic |
| TACAAAGAGGCTTGAGGTTG+AGG | 0.407050 | 4.4:-33755368 | MS.gene052334:CDS |
| TGCAGTAGCTAGCACCATTA+GGG | 0.411846 | 4.4:+33755954 | None:intergenic |
| GCAGAAGCATCACCGTATAA+AGG | 0.414708 | 4.4:+33755221 | None:intergenic |
| TATTTGGTAAGCCTGCCTTT+AGG | 0.415201 | 4.4:-33755930 | MS.gene052334:CDS |
| GTCAAACATGCTTACCAGCT+TGG | 0.416717 | 4.4:+33760151 | None:intergenic |
| GCCGTACATAAATATTTGTA+AGG | 0.419698 | 4.4:+33754176 | None:intergenic |
| AGAAATTAGGGATTAAGAAG+AGG | 0.424819 | 4.4:-33764682 | MS.gene052334:CDS |
| TCGACTGCTGGTTCGAAAGC+GGG | 0.425675 | 4.4:-33757167 | MS.gene052334:CDS |
| TATACCTTGCCTTCTTCCTC+AGG | 0.425776 | 4.4:+33753807 | None:intergenic |
| AAAACAATAAACAGACGTAC+TGG | 0.429784 | 4.4:-33758835 | MS.gene052334:CDS |
| CATCATGTCACGCGCCGATT+CGG | 0.429872 | 4.4:-33764746 | MS.gene052334:CDS |
| CACATTCTTATCCATACTGT+TGG | 0.432613 | 4.4:-33760207 | MS.gene052334:CDS |
| ACCAATAGCCTCTCTGATAT+AGG | 0.435886 | 4.4:+33749179 | None:intergenic |
| TGTGCTTAAGTTAGGGATTG+GGG | 0.436310 | 4.4:-33764654 | MS.gene052334:CDS |
| GGAAATGCCATGTACACTTC+AGG | 0.437629 | 4.4:+33763039 | None:intergenic |
| TACATGGCATTTCCAGTGTT+GGG | 0.437854 | 4.4:-33763030 | MS.gene052334:CDS |
| TCCGTCTCAGTCTTACCATC+AGG | 0.438266 | 4.4:+33751027 | None:intergenic |
| GATGTGCTTAAGTTAGGGAT+TGG | 0.439528 | 4.4:-33764656 | MS.gene052334:CDS |
| TGCTAAGGATCTTGTTTACA+AGG | 0.439926 | 4.4:-33764474 | MS.gene052334:CDS |
| TCTGAGTTTCAAGAGGGAAA+TGG | 0.441304 | 4.4:-33748167 | MS.gene052334:CDS |
| CAAATTAGATTCCTAAAGGC+AGG | 0.442244 | 4.4:+33755919 | None:intergenic |
| TGATGATACATCAATCTCTC+AGG | 0.442795 | 4.4:-33750456 | MS.gene052334:CDS |
| TGAAAAGCTTTCAGGTGTAA+AGG | 0.444785 | 4.4:-33758047 | MS.gene052334:intron |
| GGGCTTGTCCTGGCTGAAGA+TGG | 0.445251 | 4.4:-33755892 | MS.gene052334:CDS |
| TACGACCTTTCAGTCTCTTA+CGG | 0.446489 | 4.4:+33754149 | None:intergenic |
| CCGTCGTAGAAGATGTATTC+GGG | 0.448451 | 4.4:+33764860 | None:intergenic |
| ACCGTCGTAGAAGATGTATT+CGG | 0.449180 | 4.4:+33764859 | None:intergenic |
| ATTCTCAGGGGCTTCATTAG+TGG | 0.451665 | 4.4:-33762593 | MS.gene052334:CDS |
| TCTGTAAAGTAATCAAATAA+TGG | 0.453348 | 4.4:+33762471 | None:intergenic |
| CATCCTCACTAATCCATAAA+GGG | 0.453958 | 4.4:+33758107 | None:intergenic |
| GATGATGACAATAATTGATC+TGG | 0.455734 | 4.4:-33752820 | MS.gene052334:CDS |
| ACGTATCATTACATGGATCA+AGG | 0.458544 | 4.4:+33751845 | None:intergenic |
| GTATCAGATCTCCAACAGTA+TGG | 0.458950 | 4.4:+33760196 | None:intergenic |
| ATCATCAGTTACATAATTGT+CGG | 0.459807 | 4.4:+33762419 | None:intergenic |
| TTAGGAATCTAATTTGCAAC+GGG | 0.462236 | 4.4:-33755912 | MS.gene052334:CDS |
| TGATAGTTCCGGCGAGGATG+TGG | 0.466382 | 4.4:+33764804 | None:intergenic |
| TACTTTAACGTATCATTACA+TGG | 0.470875 | 4.4:+33751838 | None:intergenic |
| TTTGTTATCTTGTAAAGTGA+TGG | 0.471584 | 4.4:-33753922 | MS.gene052334:CDS |
| AAGGGTAGGCTAGTTAAGTC+TGG | 0.472831 | 4.4:-33760238 | MS.gene052334:CDS |
| GAAATTAGGGATTAAGAAGA+GGG | 0.475664 | 4.4:-33764681 | MS.gene052334:CDS |
| GTCGCTGAAAGAGAAATTGT+TGG | 0.477111 | 4.4:-33758858 | MS.gene052334:CDS |
| TGACATTAATGGAAAGTTGA+AGG | 0.477800 | 4.4:-33752616 | MS.gene052334:CDS |
| ACCTGAAAATAGTGAAAGAA+TGG | 0.477928 | 4.4:+33748355 | None:intergenic |
| TTCTCAGGGGCTTCATTAGT+GGG | 0.478440 | 4.4:-33762592 | MS.gene052334:CDS |
| AATTTGCAACGGGCTTGTCC+TGG | 0.482373 | 4.4:-33755902 | MS.gene052334:CDS |
| TCCCCTGGTATAATGCATAT+AGG | 0.487355 | 4.4:-33755405 | MS.gene052334:CDS |
| TACGTGCTGAGCCTGACTTT+AGG | 0.488259 | 4.4:-33751807 | MS.gene052334:intron |
| GTTGTAGCTGTTGACGATGA+TGG | 0.490122 | 4.4:-33760890 | MS.gene052334:CDS |
| TTCATCTTTCCTTAGGCAAA+GGG | 0.492442 | 4.4:-33760256 | MS.gene052334:intron |
| GGCTTGTCCTGGCTGAAGAT+GGG | 0.492484 | 4.4:-33755891 | MS.gene052334:CDS |
| GCCTGATCAAGGAGTACAAA+AGG | 0.494268 | 4.4:+33755337 | None:intergenic |
| CCGAGTACCACTTCTGCAGC+AGG | 0.494810 | 4.4:+33748033 | None:intergenic |
| TAGGTTATGCCATATAGTAC+TGG | 0.495321 | 4.4:-33763630 | MS.gene052334:CDS |
| GCTTTCGCAAACGTCTTCCA+TGG | 0.495420 | 4.4:+33764979 | None:intergenic |
| GCTGGAAAATGCAAGAGATT+GGG | 0.496718 | 4.4:-33758164 | MS.gene052334:CDS |
| AATATGCGAAAAGCTTGTAA+TGG | 0.497580 | 4.4:-33753861 | MS.gene052334:CDS |
| TGTCACGCGCCGATTCGGTT+GGG | 0.498641 | 4.4:-33764741 | MS.gene052334:CDS |
| ACATGACGAGTCATTATTTG+AGG | 0.498938 | 4.4:-33750803 | MS.gene052334:CDS |
| ATTGGGAGAATGTGATTACT+AGG | 0.499492 | 4.4:-33764586 | MS.gene052334:CDS |
| TAAGCGCTTTCACAATTGGC+TGG | 0.500200 | 4.4:-33758182 | MS.gene052334:CDS |
| ATTGGTGAAGAGAGCTTCCA+TGG | 0.500730 | 4.4:-33748440 | MS.gene052334:CDS |
| CGAAATGCATACCTAAAGTC+AGG | 0.501793 | 4.4:+33751796 | None:intergenic |
| TCCTATATCAGAGAGGCTAT+TGG | 0.503784 | 4.4:-33749180 | MS.gene052334:CDS |
| GGATTGAGCAAAGTGTCTTA+AGG | 0.503867 | 4.4:-33752842 | MS.gene052334:CDS |
| CTTTCCGGACAAAATGAACA+AGG | 0.504134 | 4.4:+33755260 | None:intergenic |
| ACTTCAGTGTGGCCTGATCA+AGG | 0.506854 | 4.4:+33755326 | None:intergenic |
| CTTTACAGAGTTATCTGATA+CGG | 0.507196 | 4.4:-33762457 | MS.gene052334:CDS |
| TTGAATGATCTCTTGATAGC+AGG | 0.508251 | 4.4:+33748198 | None:intergenic |
| GACGAGTCATTATTTGAGGC+TGG | 0.509581 | 4.4:-33750799 | MS.gene052334:CDS |
| AAATCATCTTCACCAAAAGC+AGG | 0.511257 | 4.4:+33762369 | None:intergenic |
| AGTGGCTCAGCACGCACGAC+AGG | 0.512457 | 4.4:+33755603 | None:intergenic |
| AAAGTCAGGCTCAGCACGTA+AGG | 0.513638 | 4.4:+33751810 | None:intergenic |
| GTTGCAAATTAGATTCCTAA+AGG | 0.514094 | 4.4:+33755915 | None:intergenic |
| CATGACAATCCCAACCGAAT+CGG | 0.514655 | 4.4:+33764732 | None:intergenic |
| GTGAGGGAGACCGGTGGCGA+AGG | 0.515091 | 4.4:+33764832 | None:intergenic |
| TCTTGTTTACAAGGGTTTCA+AGG | 0.515226 | 4.4:-33764465 | MS.gene052334:intron |
| ACCTTACAAATATTTATGTA+CGG | 0.516720 | 4.4:-33754177 | MS.gene052334:CDS |
| CAGCCTCAAAATTAGACAGC+GGG | 0.516974 | 4.4:+33763598 | None:intergenic |
| CCATGTTGATCGCCCAACAC+TGG | 0.518071 | 4.4:+33763018 | None:intergenic |
| ACAAAGAGGCTTGAGGTTGA+GGG | 0.522543 | 4.4:-33755367 | MS.gene052334:CDS |
| ATGTCACGCGCCGATTCGGT+TGG | 0.522762 | 4.4:-33764742 | MS.gene052334:CDS |
| CGTGACATGATGACCGGTCA+TGG | 0.523237 | 4.4:+33764757 | None:intergenic |
| GGATAATGGATATACGCATA+AGG | 0.523598 | 4.4:+33757138 | None:intergenic |
| GTAACTGATGATAGTGGTAC+TGG | 0.524641 | 4.4:-33762408 | MS.gene052334:CDS |
| GTGGCCGTAGTGAGGGAGAC+CGG | 0.525617 | 4.4:+33764823 | None:intergenic |
| GAGACGGAGATTGATGCTGC+TGG | 0.527027 | 4.4:-33751012 | MS.gene052334:CDS |
| GGGAGACCGGTGGCGAAGGG+TGG | 0.527778 | 4.4:+33764836 | None:intergenic |
| TTCTTTCTACTACAGGGAGA+TGG | 0.530880 | 4.4:-33752670 | MS.gene052334:intron |
| ACAGCAACCTGCTGCAGAAG+TGG | 0.532880 | 4.4:-33748040 | MS.gene052334:CDS |
| GTACATGGCATTTCCAGTGT+TGG | 0.533037 | 4.4:-33763031 | MS.gene052334:CDS |
| TTTACAGAGCTGTTCCAAGC+TGG | 0.533822 | 4.4:-33760165 | MS.gene052334:intron |
| CGATTCGGTTGGGATTGTCA+TGG | 0.536065 | 4.4:-33764731 | MS.gene052334:CDS |
| AATTATGTAACTGATGATAG+TGG | 0.536542 | 4.4:-33762414 | MS.gene052334:CDS |
| TTTAGGAATCTAATTTGCAA+CGG | 0.536781 | 4.4:-33755913 | MS.gene052334:CDS |
| GACGGTCCACCCTTCGCCAC+CGG | 0.537588 | 4.4:-33764842 | MS.gene052334:CDS |
| AAGAGACTGAAAGGTCGTAC+TGG | 0.539021 | 4.4:-33754145 | MS.gene052334:CDS |
| ATTACTATTCAACATGAAAG+TGG | 0.540052 | 4.4:-33757578 | MS.gene052334:CDS |
| TGAGGGAGACCGGTGGCGAA+GGG | 0.541814 | 4.4:+33764833 | None:intergenic |
| CCTTCAGCTACAAAATGGCC+AGG | 0.545181 | 4.4:+33757081 | None:intergenic |
| CGCTCCATAATCATCAATAA+CGG | 0.545617 | 4.4:+33755825 | None:intergenic |
| ACACACAAAGCAAGGTTGCT+AGG | 0.546559 | 4.4:+33762955 | None:intergenic |
| AAACAACTTACTGCATGTGC+AGG | 0.548444 | 4.4:+33749129 | None:intergenic |
| GTTTCAAGAGGGAAATGGAA+AGG | 0.548912 | 4.4:-33748162 | MS.gene052334:intron |
| TTCGACTGCTGGTTCGAAAG+CGG | 0.549071 | 4.4:-33757168 | MS.gene052334:CDS |
| CACTAATCCATAAAGGGAGA+GGG | 0.549431 | 4.4:+33758113 | None:intergenic |
| CGAGGATGTGGCCGTAGTGA+GGG | 0.549436 | 4.4:+33764816 | None:intergenic |
| GAACAGCTCTGTAAATGAGT+GGG | 0.550472 | 4.4:+33760173 | None:intergenic |
| CAGGTACGCAATAAGTATAC+GGG | 0.555305 | 4.4:-33762787 | MS.gene052334:CDS |
| TAATCATCAATAACGGCCAT+TGG | 0.556040 | 4.4:+33755832 | None:intergenic |
| CCTTCCTTCTTGAAGCGAAG+TGG | 0.557494 | 4.4:+33755585 | None:intergenic |
| TCAAAATACAAAGAGGCTTG+AGG | 0.558853 | 4.4:-33755374 | MS.gene052334:CDS |
| AGGAAGAGTCCACGGTGTTG+TGG | 0.558860 | 4.4:+33762975 | None:intergenic |
| AAGACATGTTTGAAATCCCA+TGG | 0.559387 | 4.4:+33748423 | None:intergenic |
| GAGATTGATGCTGCTGGAGA+TGG | 0.561468 | 4.4:-33751006 | MS.gene052334:intron |
| GAGAATGTGATTACTAGGAC+TGG | 0.561536 | 4.4:-33764581 | MS.gene052334:CDS |
| GCGAGGATGTGGCCGTAGTG+AGG | 0.562268 | 4.4:+33764815 | None:intergenic |
| CACTACGGCCACATCCTCGC+CGG | 0.562709 | 4.4:-33764812 | MS.gene052334:CDS |
| TCAATAACGGCCATTGGTGA+AGG | 0.565741 | 4.4:+33755838 | None:intergenic |
| TCAGTAAAGAACGGTGTGAA+AGG | 0.566119 | 4.4:+33753897 | None:intergenic |
| ATGTGCTGGAGAAATTCTCA+GGG | 0.569997 | 4.4:-33762606 | MS.gene052334:CDS |
| CGTATCATTACATGGATCAA+GGG | 0.570865 | 4.4:+33751846 | None:intergenic |
| TGAGCCAACTGACACAGTTG+AGG | 0.570906 | 4.4:-33750498 | MS.gene052334:CDS |
| GGGAACTTCAAGTGGAAAGA+CGG | 0.571657 | 4.4:-33762650 | MS.gene052334:CDS |
| ATCTGATCCTGAAGTGTACA+TGG | 0.572330 | 4.4:-33763046 | MS.gene052334:CDS |
| CATCAAATGTTCTTGATCAG+TGG | 0.573756 | 4.4:-33755303 | MS.gene052334:CDS |
| GAACCTATATGCATTATACC+AGG | 0.573904 | 4.4:+33755402 | None:intergenic |
| GGGTTTATAATGAAGAGTGT+AGG | 0.574940 | 4.4:-33764634 | MS.gene052334:CDS |
| TCACTAATCCATAAAGGGAG+AGG | 0.575867 | 4.4:+33758112 | None:intergenic |
| TGATTACTAGGACTGGAAGA+TGG | 0.576110 | 4.4:-33764574 | MS.gene052334:CDS |
| TTGCAAATCAATGTTTGAAG+CGG | 0.576220 | 4.4:-33751597 | MS.gene052334:CDS |
| CATCAGGGCGTTTAAAATCA+CGG | 0.578957 | 4.4:+33751043 | None:intergenic |
| ACGCGCTACCAGTCCATGAC+CGG | 0.579636 | 4.4:-33764770 | MS.gene052334:CDS |
| CACCAAAAGCAGGGGCACAA+TGG | 0.580369 | 4.4:+33762379 | None:intergenic |
| TTGAAGTCTGAGTTTCAAGA+GGG | 0.581600 | 4.4:-33748173 | MS.gene052334:CDS |
| TGGCATTGACACACAAAGCA+AGG | 0.581794 | 4.4:+33762947 | None:intergenic |
| GGATATCATTGAAGCAGCAA+AGG | 0.582561 | 4.4:-33760810 | MS.gene052334:intron |
| CATGATGACCGGTCATGGAC+TGG | 0.582856 | 4.4:+33764762 | None:intergenic |
| CGATGTTTGACCAGAATGAG+AGG | 0.583134 | 4.4:+33764921 | None:intergenic |
| AATCATCTTCACCAAAAGCA+GGG | 0.585583 | 4.4:+33762370 | None:intergenic |
| AAGACCAAGGGAACTTCAAG+TGG | 0.586594 | 4.4:-33762658 | MS.gene052334:CDS |
| CCGTCTCAGTCTTACCATCA+GGG | 0.587414 | 4.4:+33751028 | None:intergenic |
| CCTGCTGCAGAAGTGGTACT+CGG | 0.587902 | 4.4:-33748033 | MS.gene052334:CDS |
| CCACTTCGCTTCAAGAAGGA+AGG | 0.588148 | 4.4:-33755585 | MS.gene052334:CDS |
| GATATAGCACCCACTGAAGT+CGG | 0.588542 | 4.4:+33760847 | None:intergenic |
| TGAGGCTGGTGCTGCGAGAG+AGG | 0.588628 | 4.4:-33750785 | MS.gene052334:intron |
| AGAAGTCAAAGCAATGTCAC+AGG | 0.595546 | 4.4:-33751659 | MS.gene052334:CDS |
| TTTGAAGTCTGAGTTTCAAG+AGG | 0.595806 | 4.4:-33748174 | MS.gene052334:CDS |
| TCCTTGTTCAAAATACAAAG+AGG | 0.596204 | 4.4:-33755381 | MS.gene052334:CDS |
| CTATATCAAACAAGCCGACA+AGG | 0.599329 | 4.4:-33760831 | MS.gene052334:CDS |
| TAAAGAAACTGACCAACCAC+GGG | 0.599523 | 4.4:+33757047 | None:intergenic |
| TGCGTTACAGTATGTACTTG+AGG | 0.600275 | 4.4:-33751884 | MS.gene052334:intron |
| GCTAAGGATCTTGTTTACAA+GGG | 0.600479 | 4.4:-33764473 | MS.gene052334:CDS |
| ACTTTCCATTAATGTCAGCA+AGG | 0.601867 | 4.4:+33752622 | None:intergenic |
| ATGGATATACGCATAAGGCA+TGG | 0.603279 | 4.4:+33757143 | None:intergenic |
| CATCAACGCGTCGAAGCACA+CGG | 0.603542 | 4.4:+33757555 | None:intergenic |
| CTCAACCGTAAGAGACTGAA+AGG | 0.604341 | 4.4:-33754154 | MS.gene052334:CDS |
| CCCAAAGGATAAACCCAAAG+AGG | 0.604507 | 4.4:-33762719 | MS.gene052334:CDS |
| ATAAAGAAACTGACCAACCA+CGG | 0.605026 | 4.4:+33757046 | None:intergenic |
| GACTGGATCAAACCCGTGGT+TGG | 0.605146 | 4.4:-33757059 | MS.gene052334:intron |
| GATGATACATCAATCTCTCA+GGG | 0.606020 | 4.4:-33750455 | MS.gene052334:CDS |
| AAACAATAAACAGACGTACT+GGG | 0.607289 | 4.4:-33758834 | MS.gene052334:CDS |
| TGAGCCACTTCGCTTCAAGA+AGG | 0.609603 | 4.4:-33755589 | MS.gene052334:CDS |
| GATCAATGTTGTGTCGATGA+AGG | 0.611059 | 4.4:+33757603 | None:intergenic |
| TCGGCGCGTGACATGATGAC+CGG | 0.611449 | 4.4:+33764751 | None:intergenic |
| TGACAATCAAATTCTTACCA+AGG | 0.612133 | 4.4:-33762334 | MS.gene052334:intron |
| TAAACCTCAACTGTGTCAGT+TGG | 0.612355 | 4.4:+33750494 | None:intergenic |
| GTACTGGAGAAGAAGACTGC+AGG | 0.613262 | 4.4:-33754129 | MS.gene052334:CDS |
| CCCGAATACATCTTCTACGA+CGG | 0.614903 | 4.4:-33764860 | MS.gene052334:CDS |
| TTTCACACCGTTCTTTACTG+AGG | 0.617547 | 4.4:-33753895 | MS.gene052334:CDS |
| GGAACAGCTCTGTAAATGAG+TGG | 0.618149 | 4.4:+33760172 | None:intergenic |
| AACCTATATGCATTATACCA+GGG | 0.618966 | 4.4:+33755403 | None:intergenic |
| AAGAGACTAGGAAAATCTAT+GGG | 0.619964 | 4.4:-33751694 | MS.gene052334:CDS |
| CCAGTGTTGGGCGATCAACA+TGG | 0.620219 | 4.4:-33763018 | MS.gene052334:CDS |
| GTTTATAAGGTACAATCGCA+AGG | 0.620671 | 4.4:+33755629 | None:intergenic |
| ATTGTACCTTGACGAAATCA+GGG | 0.622064 | 4.4:+33758810 | None:intergenic |
| TCTCCCTTTATGGATTAGTG+AGG | 0.625202 | 4.4:-33758110 | MS.gene052334:CDS |
| CTGGGTCCCTGATTTCGTCA+AGG | 0.633297 | 4.4:-33758816 | MS.gene052334:intron |
| CCCTGATGGTAAGACTGAGA+CGG | 0.635377 | 4.4:-33751028 | MS.gene052334:CDS |
| TCTTTCCTTAGGCAAAGGGT+AGG | 0.635798 | 4.4:-33760252 | MS.gene052334:intron |
| TATATGCATTATACCAGGGG+AGG | 0.637266 | 4.4:+33755407 | None:intergenic |
| AAAAGCTTGTAATGGATCAG+AGG | 0.637890 | 4.4:-33753853 | MS.gene052334:CDS |
| TTGGTGAAGAGAGCTTCCAT+GGG | 0.639172 | 4.4:-33748439 | MS.gene052334:CDS |
| ACCTATATGCATTATACCAG+GGG | 0.646802 | 4.4:+33755404 | None:intergenic |
| GTCTACACTCCATAACCCAA+AGG | 0.647106 | 4.4:-33762734 | MS.gene052334:CDS |
| ACTAATCCATAAAGGGAGAG+GGG | 0.647380 | 4.4:+33758114 | None:intergenic |
| ATCATCTTCACCAAAAGCAG+GGG | 0.649136 | 4.4:+33762371 | None:intergenic |
| TACAGTACCTCAGTAAAGAA+CGG | 0.650380 | 4.4:+33753888 | None:intergenic |
| CGTTATTGATGATTATGGAG+CGG | 0.650729 | 4.4:-33755824 | MS.gene052334:intron |
| TCTCAGGGGCTTCATTAGTG+GGG | 0.664387 | 4.4:-33762591 | MS.gene052334:CDS |
| TGGTTCGTGAGAGCTGTGTG+TGG | 0.665601 | 4.4:+33764891 | None:intergenic |
| AGCCTCTTAAAACACCACTC+AGG | 0.667608 | 4.4:-33752770 | MS.gene052334:intron |
| AGTGTGTTAGGTAAGAGACT+AGG | 0.669040 | 4.4:-33751706 | MS.gene052334:CDS |
| GCCGTAGTGAGGGAGACCGG+TGG | 0.670115 | 4.4:+33764826 | None:intergenic |
| TGATACATCAATCTCTCAGG+GGG | 0.670853 | 4.4:-33750453 | MS.gene052334:CDS |
| AACAGCTCTGTAAATGAGTG+GGG | 0.675850 | 4.4:+33760174 | None:intergenic |
| GCCACCGGTCTCCCTCACTA+CGG | 0.680699 | 4.4:-33764827 | MS.gene052334:CDS |
| CATTAATGGAAAGTTGAAGG+AGG | 0.690571 | 4.4:-33752613 | MS.gene052334:intron |
| GAAGGACTGGATCAAACCCG+TGG | 0.695082 | 4.4:-33757063 | MS.gene052334:CDS |
| TGTCTTTGATAGTTCCGGCG+AGG | 0.698547 | 4.4:+33764798 | None:intergenic |
| AGCCTCAAAATTAGACAGCG+GGG | 0.698651 | 4.4:+33763599 | None:intergenic |
| TGCACATTAATCTACCTGAG+TGG | 0.700352 | 4.4:+33752756 | None:intergenic |
| AACATTTGATGACTTCAGTG+TGG | 0.704800 | 4.4:+33755315 | None:intergenic |
| AGGTTGCTAGGAAGAGTCCA+CGG | 0.706962 | 4.4:+33762967 | None:intergenic |
| TGGCTTGGACCACAACACCG+TGG | 0.710870 | 4.4:-33762984 | MS.gene052334:CDS |
| GAAGCATCACCGTATAAAGG+CGG | 0.711620 | 4.4:+33755224 | None:intergenic |
| ATGATACATCAATCTCTCAG+GGG | 0.727659 | 4.4:-33750454 | MS.gene052334:CDS |
| CCAGCCTCAAAATTAGACAG+CGG | 0.734462 | 4.4:+33763597 | None:intergenic |
| GAACACGTGTTTCTCACAGT+AGG | 0.735096 | 4.4:-33748009 | MS.gene052334:CDS |
| GCAGGAATCCTATATCAGAG+AGG | 0.739332 | 4.4:-33749187 | MS.gene052334:intron |
| TGTGCTGGAGAAATTCTCAG+GGG | 0.747497 | 4.4:-33762605 | MS.gene052334:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 33747978 | 33764999 | 33747978 | ID=MS.gene052334 |
| chr4.4 | mRNA | 33747978 | 33764999 | 33747978 | ID=MS.gene052334.t1;Parent=MS.gene052334 |
| chr4.4 | exon | 33764466 | 33764999 | 33764466 | ID=MS.gene052334.t1.exon1;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33764466 | 33764999 | 33764466 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33763584 | 33763649 | 33763584 | ID=MS.gene052334.t1.exon2;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33763584 | 33763649 | 33763584 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33762930 | 33763073 | 33762930 | ID=MS.gene052334.t1.exon3;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33762930 | 33763073 | 33762930 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33762586 | 33762806 | 33762586 | ID=MS.gene052334.t1.exon4;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33762586 | 33762806 | 33762586 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33762335 | 33762500 | 33762335 | ID=MS.gene052334.t1.exon5;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33762335 | 33762500 | 33762335 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33760811 | 33760921 | 33760811 | ID=MS.gene052334.t1.exon6;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33760811 | 33760921 | 33760811 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33760166 | 33760263 | 33760166 | ID=MS.gene052334.t1.exon7;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33760166 | 33760263 | 33760166 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33758817 | 33758895 | 33758817 | ID=MS.gene052334.t1.exon8;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33758817 | 33758895 | 33758817 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33758048 | 33758206 | 33758048 | ID=MS.gene052334.t1.exon9;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33758048 | 33758206 | 33758048 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33757553 | 33757633 | 33757553 | ID=MS.gene052334.t1.exon10;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33757553 | 33757633 | 33757553 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33757060 | 33757193 | 33757060 | ID=MS.gene052334.t1.exon11;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33757060 | 33757193 | 33757060 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33755825 | 33755984 | 33755825 | ID=MS.gene052334.t1.exon12;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33755825 | 33755984 | 33755825 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33755572 | 33755655 | 33755572 | ID=MS.gene052334.t1.exon13;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33755572 | 33755655 | 33755572 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33755234 | 33755443 | 33755234 | ID=MS.gene052334.t1.exon14;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33755234 | 33755443 | 33755234 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33754105 | 33754230 | 33754105 | ID=MS.gene052334.t1.exon15;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33754105 | 33754230 | 33754105 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33753812 | 33753949 | 33753812 | ID=MS.gene052334.t1.exon16;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33753812 | 33753949 | 33753812 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33752771 | 33752874 | 33752771 | ID=MS.gene052334.t1.exon17;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33752771 | 33752874 | 33752771 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33752614 | 33752677 | 33752614 | ID=MS.gene052334.t1.exon18;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33752614 | 33752677 | 33752614 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33751808 | 33751896 | 33751808 | ID=MS.gene052334.t1.exon19;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33751808 | 33751896 | 33751808 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33751585 | 33751726 | 33751585 | ID=MS.gene052334.t1.exon20;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33751585 | 33751726 | 33751585 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33751007 | 33751070 | 33751007 | ID=MS.gene052334.t1.exon21;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33751007 | 33751070 | 33751007 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33750786 | 33750853 | 33750786 | ID=MS.gene052334.t1.exon22;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33750786 | 33750853 | 33750786 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33750439 | 33750561 | 33750439 | ID=MS.gene052334.t1.exon23;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33750439 | 33750561 | 33750439 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33749140 | 33749205 | 33749140 | ID=MS.gene052334.t1.exon24;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33749140 | 33749205 | 33749140 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33748357 | 33748468 | 33748357 | ID=MS.gene052334.t1.exon25;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33748357 | 33748468 | 33748357 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33748163 | 33748254 | 33748163 | ID=MS.gene052334.t1.exon26;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33748163 | 33748254 | 33748163 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| chr4.4 | exon | 33747978 | 33748082 | 33747978 | ID=MS.gene052334.t1.exon27;Parent=MS.gene052334.t1 |
| chr4.4 | CDS | 33747978 | 33748082 | 33747978 | ID=cds.MS.gene052334.t1;Parent=MS.gene052334.t1 |
| Gene Sequence |
| Protein sequence |
