AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052399

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052399.t1 MTR_6g084710 97.877 424 9 0 15 438 146 569 0.0 857
MS.gene052399.t1 MTR_6g084710 97.753 356 8 0 15 370 146 501 0.0 722
MS.gene052399.t1 MTR_1g029810 44.855 379 194 6 45 419 42 409 3.49e-98 300
MS.gene052399.t1 MTR_1g029810 44.855 379 194 6 45 419 42 409 1.22e-97 300
MS.gene052399.t1 MTR_8g094590 44.211 380 194 10 45 419 58 424 9.90e-97 297
MS.gene052399.t1 MTR_3g096930 44.562 377 196 10 45 419 69 434 6.96e-95 293
MS.gene052399.t1 MTR_4g023650 42.228 386 200 10 45 419 46 419 6.51e-93 287
MS.gene052399.t1 MTR_4g023630 40.979 388 202 11 45 419 46 419 4.18e-90 280
MS.gene052399.t1 MTR_8g094590 44.290 359 182 10 45 398 58 403 5.22e-89 276
MS.gene052399.t1 MTR_8g094590 43.893 262 131 8 45 302 58 307 9.12e-63 205
MS.gene052399.t1 MTR_3g006820 31.218 394 226 15 45 419 45 412 4.52e-48 170
MS.gene052399.t1 MTR_1g061940 27.820 399 242 13 49 421 68 446 7.44e-39 147
MS.gene052399.t1 MTR_7g102750 27.990 393 237 14 56 425 77 446 1.65e-35 137
MS.gene052399.t1 MTR_2g084550 26.744 430 262 13 17 421 38 439 3.70e-35 136
MS.gene052399.t1 MTR_4g056420 26.942 412 253 16 49 435 64 452 4.72e-35 135
MS.gene052399.t1 MTR_8g075090 28.232 379 222 14 60 419 112 459 5.06e-35 135
MS.gene052399.t1 MTR_8g075090 28.232 379 222 14 60 419 112 459 5.32e-35 136
MS.gene052399.t1 MTR_7g080230 31.156 398 232 19 50 425 59 436 1.65e-34 134
MS.gene052399.t1 MTR_5g022880 28.307 378 225 15 60 419 112 461 1.72e-32 129
MS.gene052399.t1 MTR_5g016260 30.214 374 221 13 58 419 86 431 3.47e-32 129
MS.gene052399.t1 MTR_7g021510 27.778 414 229 16 49 425 59 439 3.89e-32 127
MS.gene052399.t1 MTR_5g016260 30.214 374 221 13 58 419 86 431 1.03e-31 128
MS.gene052399.t1 MTR_4g095270 29.333 375 225 13 58 420 78 424 2.22e-31 126
MS.gene052399.t1 MTR_4g095270 28.800 375 227 12 58 420 78 424 2.56e-31 127
MS.gene052399.t1 MTR_7g102750 28.313 332 200 11 56 368 77 389 2.51e-29 119
MS.gene052399.t1 MTR_5g061690 26.884 398 237 15 60 437 101 464 1.57e-28 118
MS.gene052399.t1 MTR_8g075090 28.116 345 203 13 60 385 112 430 3.36e-28 116
MS.gene052399.t1 MTR_5g080960 27.014 422 261 18 13 419 42 431 1.33e-27 114
MS.gene052399.t1 MTR_5g080890 27.014 422 261 18 13 419 42 431 1.73e-27 114
MS.gene052399.t1 MTR_2g059460 24.760 416 255 13 22 419 29 404 3.72e-27 112
MS.gene052399.t1 MTR_8g096990 27.083 384 229 15 54 419 79 429 4.50e-26 110
MS.gene052399.t1 MTR_8g075010 25.587 383 229 14 60 419 100 449 1.10e-25 109
MS.gene052399.t1 MTR_1g078240 27.047 403 253 15 45 425 52 435 1.27e-25 109
MS.gene052399.t1 MTR_3g087780 27.344 384 223 15 60 419 99 450 3.04e-25 108
MS.gene052399.t1 MTR_3g028120 25.266 376 245 13 56 419 85 436 1.05e-24 106
MS.gene052399.t1 MTR_2g059490 25.686 401 260 11 32 419 33 408 1.09e-24 106
MS.gene052399.t1 MTR_5g044770 25.995 377 230 13 59 419 85 428 1.53e-24 105
MS.gene052399.t1 MTR_1g078240 26.980 404 253 16 45 425 52 436 3.17e-24 105
MS.gene052399.t1 MTR_5g008070 27.381 420 240 19 18 419 52 424 3.57e-24 104
MS.gene052399.t1 MTR_5g008150 27.368 380 226 17 59 419 62 410 1.27e-23 102
MS.gene052399.t1 MTR_7g117430 26.494 385 235 17 51 419 132 484 1.91e-23 102
MS.gene052399.t1 MTR_8g038310 23.622 381 222 15 59 422 86 414 2.39e-22 99.0
MS.gene052399.t1 MTR_5g008110 27.177 379 227 17 59 419 66 413 2.91e-22 98.6
MS.gene052399.t1 MTR_6g085010 24.933 373 228 16 61 419 157 491 8.77e-22 97.8
MS.gene052399.t1 MTR_1g078240 29.286 280 172 11 45 304 52 325 9.08e-22 96.7
MS.gene052399.t1 MTR_4g007740 26.402 428 268 18 11 419 78 477 1.51e-21 97.1
MS.gene052399.t1 MTR_5g038360 23.720 371 246 12 61 419 89 434 6.79e-21 94.7
MS.gene052399.t1 MTR_2g028190 24.869 382 237 13 58 419 100 451 8.53e-21 94.7
MS.gene052399.t1 MTR_1g115210 25.581 387 233 17 51 419 137 486 1.04e-19 91.7
MS.gene052399.t1 MTR_3g097150 26.498 434 251 21 6 419 61 446 1.37e-19 90.9
MS.gene052399.t1 MTR_3g053980 26.632 383 235 15 52 419 134 485 1.43e-19 91.3
MS.gene052399.t1 MTR_4g045857 29.114 237 152 7 50 278 51 279 3.62e-19 87.8
MS.gene052399.t1 MTR_0036s0050 26.385 379 227 16 59 419 89 433 2.03e-18 87.4
MS.gene052399.t1 MTR_3g053710 24.096 415 259 16 25 419 88 466 3.02e-18 87.0
MS.gene052399.t1 MTR_1g111350 24.282 383 239 16 51 419 122 467 3.97e-18 86.7
MS.gene052399.t1 MTR_3g046300 23.992 471 277 19 3 419 15 458 1.27e-16 82.0
MS.gene052399.t1 MTR_5g091130 27.041 392 229 18 59 420 190 554 1.43e-15 79.3
MS.gene052399.t1 MTR_5g044570 23.077 364 231 12 61 419 90 409 3.98e-15 77.4
MS.gene052399.t1 MTR_1g039270 23.980 392 234 18 51 419 119 469 5.45e-15 77.0
MS.gene052399.t1 MTR_6g090290 23.474 426 274 16 13 419 42 434 7.60e-15 76.6
MS.gene052399.t1 MTR_4g109760 25.348 359 215 13 79 419 73 396 1.31e-13 72.4
MS.gene052399.t1 MTR_6g088760 24.038 312 189 12 50 331 78 371 1.59e-13 72.8
MS.gene052399.t1 MTR_3g068000 24.079 407 238 21 65 431 78 453 2.43e-13 72.0
MS.gene052399.t1 MTR_7g062360 19.036 394 275 11 57 421 49 427 1.04e-11 67.0
MS.gene052399.t1 MTR_5g072060 35.135 111 67 2 135 240 389 499 5.58e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052399.t1 AT1G49050 58.907 421 162 6 12 425 158 574 6.60e-177 507
MS.gene052399.t1 AT1G49050 61.394 373 137 4 60 425 29 401 9.71e-165 470
MS.gene052399.t1 AT1G49050 56.711 298 120 5 12 304 158 451 2.72e-115 346
MS.gene052399.t1 AT1G77480 46.457 381 188 8 44 419 50 419 2.19e-106 322
MS.gene052399.t1 AT1G77480 46.457 381 188 8 44 419 50 419 1.19e-105 321
MS.gene052399.t1 AT4G33490 42.195 410 221 10 14 419 14 411 4.02e-102 311
MS.gene052399.t1 AT1G44130 42.424 396 201 8 30 419 25 399 1.29e-101 309
MS.gene052399.t1 AT4G33490 43.085 376 198 10 14 385 11 374 7.69e-93 286
MS.gene052399.t1 AT5G43100 31.746 378 209 15 58 419 73 417 9.46e-43 157
MS.gene052399.t1 AT3G02740 31.458 391 226 16 49 419 71 439 4.53e-42 155
MS.gene052399.t1 AT5G43100 31.746 378 209 15 58 419 73 417 9.42e-42 157
MS.gene052399.t1 AT3G02740 31.332 383 223 15 55 419 4 364 1.92e-40 149
MS.gene052399.t1 AT5G22850 28.462 390 244 11 49 419 67 440 8.05e-40 149
MS.gene052399.t1 AT1G65240 30.542 406 228 14 45 425 56 432 6.11e-39 146
MS.gene052399.t1 AT2G36670 27.378 431 261 14 20 421 52 459 1.91e-38 145
MS.gene052399.t1 AT5G36260 30.100 402 230 16 49 425 64 439 2.83e-38 145
MS.gene052399.t1 AT2G36670 26.835 436 262 14 20 421 52 464 6.93e-37 141
MS.gene052399.t1 AT1G08210 28.061 392 244 10 49 421 70 442 8.06e-37 141
MS.gene052399.t1 AT2G36670 28.906 384 228 13 60 421 33 393 9.54e-37 140
MS.gene052399.t1 AT2G36670 28.906 384 228 13 60 421 33 393 9.54e-37 140
MS.gene052399.t1 AT3G50050 30.971 381 210 15 58 419 90 436 9.57e-37 142
MS.gene052399.t1 AT1G08210 28.061 392 244 10 49 421 104 476 1.02e-36 141
MS.gene052399.t1 AT1G08210 28.934 394 238 13 49 421 118 490 1.07e-36 141
MS.gene052399.t1 AT1G05840 30.296 406 227 19 49 425 66 444 2.53e-36 139
MS.gene052399.t1 AT1G08210 26.969 419 241 12 49 421 70 469 7.56e-32 127
MS.gene052399.t1 AT2G17760 28.836 378 220 15 60 419 103 449 2.78e-31 125
MS.gene052399.t1 AT4G35880 26.984 378 226 14 60 419 106 451 7.29e-29 119
MS.gene052399.t1 AT3G25700 24.762 420 276 16 25 422 51 452 4.08e-28 116
MS.gene052399.t1 AT4G35880 27.246 345 206 13 60 386 106 423 2.68e-25 107
MS.gene052399.t1 AT5G10080 27.415 383 227 15 60 419 99 453 9.03e-25 107
MS.gene052399.t1 AT2G28010 24.130 431 268 16 1 419 8 391 1.69e-23 102
MS.gene052399.t1 AT3G51350 24.934 381 236 15 60 419 101 452 3.17e-22 99.4
MS.gene052399.t1 AT5G10770 23.877 423 275 12 12 419 83 473 3.31e-22 99.0
MS.gene052399.t1 AT4G30030 25.229 436 261 22 8 419 25 419 1.09e-21 97.1
MS.gene052399.t1 AT2G23945 26.509 381 232 14 60 419 95 448 4.75e-21 95.5
MS.gene052399.t1 AT2G03200 25.840 387 230 16 59 420 105 459 6.94e-21 95.1
MS.gene052399.t1 AT2G28220 23.394 436 271 17 1 419 8 397 4.21e-20 92.4
MS.gene052399.t1 AT1G01300 25.907 386 238 18 51 420 132 485 1.17e-19 91.7
MS.gene052399.t1 AT2G35615 24.173 393 248 13 52 419 76 443 1.44e-19 90.9
MS.gene052399.t1 AT3G51340 23.097 381 237 9 60 419 102 447 1.61e-19 91.3
MS.gene052399.t1 AT3G20015 25.065 387 235 16 51 419 121 470 1.88e-19 90.9
MS.gene052399.t1 AT2G28225 26.410 390 219 19 50 419 56 397 3.01e-19 89.7
MS.gene052399.t1 AT1G31450 25.064 391 245 14 52 419 76 441 4.64e-19 89.4
MS.gene052399.t1 AT3G51340 23.636 385 242 12 60 419 102 459 5.90e-19 89.7
MS.gene052399.t1 AT3G51360 23.969 388 227 17 60 419 4 351 7.32e-19 88.6
MS.gene052399.t1 AT3G51360 24.485 388 225 18 60 419 88 435 2.33e-18 87.8
MS.gene052399.t1 AT3G51360 24.485 388 225 18 60 419 98 445 2.48e-18 87.4
MS.gene052399.t1 AT1G25510 26.329 395 216 21 51 419 138 483 3.03e-18 87.4
MS.gene052399.t1 AT1G64830 23.039 408 269 14 28 419 50 428 4.00e-18 86.7
MS.gene052399.t1 AT3G61820 25.964 389 238 17 51 419 125 483 6.43e-18 86.3
MS.gene052399.t1 AT2G28030 23.483 379 240 13 50 419 50 387 1.77e-17 84.3
MS.gene052399.t1 AT3G51330 23.873 377 246 13 60 419 101 453 1.85e-17 84.7
MS.gene052399.t1 AT3G51330 23.873 377 246 13 60 419 101 453 2.29e-17 84.7
MS.gene052399.t1 AT3G18490 24.934 381 242 16 51 419 152 500 2.70e-17 84.3
MS.gene052399.t1 AT3G51360 26.766 269 149 11 60 305 98 341 9.14e-17 81.6
MS.gene052399.t1 AT4G30040 24.779 339 211 15 61 375 85 403 1.42e-16 82.0
MS.gene052399.t1 AT3G25700 30.539 167 104 6 25 183 51 213 2.49e-16 80.5
MS.gene052399.t1 AT3G51360 26.866 268 148 11 60 304 98 340 4.38e-16 80.1
MS.gene052399.t1 AT3G51360 26.866 268 148 11 60 304 98 340 5.43e-16 80.1
MS.gene052399.t1 AT3G51340 26.104 249 165 6 60 297 102 342 5.72e-16 79.7
MS.gene052399.t1 AT3G51360 29.444 180 110 6 60 228 88 261 6.14e-16 79.7
MS.gene052399.t1 AT3G51360 29.444 180 110 6 60 228 88 261 7.82e-16 79.7
MS.gene052399.t1 AT3G51340 26.104 249 165 6 60 297 102 342 7.86e-16 79.3
MS.gene052399.t1 AT3G59080 25.879 398 241 17 52 420 161 533 8.66e-16 80.1
MS.gene052399.t1 AT5G33340 24.538 379 238 14 59 420 88 435 8.60e-15 76.6
MS.gene052399.t1 AT2G42980 24.311 399 248 17 52 420 151 525 9.28e-15 76.6
MS.gene052399.t1 AT3G12700 24.632 272 180 9 52 306 97 360 6.11e-14 73.9
MS.gene052399.t1 AT3G51330 26.482 253 160 9 60 297 101 342 6.23e-14 73.9
MS.gene052399.t1 AT4G12920 24.041 391 245 17 43 421 39 389 1.01e-12 69.7
MS.gene052399.t1 AT5G10760 24.250 400 245 14 36 419 107 464 1.26e-12 69.7
MS.gene052399.t1 AT1G09750 23.775 408 248 18 37 419 79 448 3.57e-11 65.5

Find 94 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCCTACTTACGTAGTT+TGG 0.207057 6.1:+74317000 None:intergenic
AACAAAATTTAACTTGGTTT+TGG 0.209893 6.1:+74317344 None:intergenic
ATGCAGCTTAAAGAAGTTTC+TGG 0.214230 6.1:-74316365 MS.gene052399:intron
GAGGGATTTGAAGCTTAAAT+TGG 0.254073 6.1:-74319250 MS.gene052399:CDS
TTCCTTAGGAGTTCTTATTA+AGG 0.264245 6.1:-74317397 MS.gene052399:CDS
CTTTATTGCTTCTAGCTCTA+AGG 0.281453 6.1:-74319199 MS.gene052399:CDS
TTATACCAAACTGAGATTCT+TGG 0.285575 6.1:-74316590 MS.gene052399:CDS
GGATAGGGTTGGCTTATATA+TGG 0.307511 6.1:+74317536 None:intergenic
CATTTCACTGCGAGGACATT+TGG 0.309662 6.1:-74315077 MS.gene052399:CDS
CTTAAAGAAGTTTCTGGGTT+GGG 0.327554 6.1:-74316359 MS.gene052399:CDS
GGCGGTGGATACATGTTCTT+AGG 0.334489 6.1:-74317068 MS.gene052399:CDS
TTTGTTGGCAGGCTAATTTC+CGG 0.346488 6.1:-74316301 MS.gene052399:CDS
AAATTAGCCTGCCAACAAAT+TGG 0.352730 6.1:+74316305 None:intergenic
GTTTGCTTAGCTATCCTTGA+TGG 0.362073 6.1:-74315839 MS.gene052399:CDS
ACATCAACAAAATATCGTTT+TGG 0.366056 6.1:+74317991 None:intergenic
CTTCATCTTATGACTACTAA+TGG 0.384187 6.1:-74317369 MS.gene052399:CDS
CTTATCAGTTAGCAAGTAAA+GGG 0.384205 6.1:-74317136 MS.gene052399:CDS
CCCGAAAACAAAATTTAACT+TGG 0.384883 6.1:+74317338 None:intergenic
AAATCCTACTTACGTAGTTT+GGG 0.388948 6.1:+74317001 None:intergenic
GACAATGTAAACAAGAATAT+TGG 0.395592 6.1:-74315046 MS.gene052399:CDS
AAGACATTAACGCTACGTTT+TGG 0.403275 6.1:-74316027 MS.gene052399:CDS
AGGGATTTGAAGCTTAAATT+GGG 0.403291 6.1:-74319249 MS.gene052399:CDS
AATGTTGTTGGTCATTGCTT+AGG 0.406763 6.1:-74317104 MS.gene052399:CDS
ACCCAAGTCATACCCCAATA+AGG 0.406933 6.1:+74317032 None:intergenic
GGCAGGCTAATTTCCGGATC+AGG 0.409326 6.1:-74316295 MS.gene052399:intron
GGGCTTATTAAGAATGTTGT+TGG 0.412532 6.1:-74317116 MS.gene052399:CDS
TTCCACGCATAGAGAGTCTA+TGG 0.414996 6.1:+74317500 None:intergenic
CCTTTACTTGCTAACTGATA+AGG 0.415422 6.1:+74317137 None:intergenic
GGATAAACATTGCCACTGAT+TGG 0.415505 6.1:+74319144 None:intergenic
TTTGATGGAAACAGCAAAGT+TGG 0.416480 6.1:-74316539 MS.gene052399:CDS
ACACAACTTTGCCAATTTGT+TGG 0.420208 6.1:-74316316 MS.gene052399:CDS
TGCAGCTTAAAGAAGTTTCT+GGG 0.429338 6.1:-74316364 MS.gene052399:intron
AGATCCAGGTACGCCTCTCT+TGG 0.432957 6.1:+74316476 None:intergenic
GTGATTTCCCTTACCTGATC+CGG 0.435772 6.1:+74316282 None:intergenic
GAAAGTACCTCCAAGTATAA+TGG 0.441127 6.1:+74315793 None:intergenic
GCAGACCATAGTTCTTCCTT+AGG 0.443156 6.1:-74317411 MS.gene052399:CDS
TGCTTAGGAAATGATGGACT+TGG 0.453335 6.1:-74317089 MS.gene052399:CDS
AACTTTGCCAATTTGTTGGC+AGG 0.453572 6.1:-74316312 MS.gene052399:CDS
TGCCTTATTGGGGTATGACT+TGG 0.456727 6.1:-74317034 MS.gene052399:CDS
TTAAAGAAGTTTCTGGGTTG+GGG 0.458580 6.1:-74316358 MS.gene052399:CDS
TTATTCCAAGAATCTCAGTT+TGG 0.460107 6.1:+74316585 None:intergenic
CCTTATCAGTTAGCAAGTAA+AGG 0.465935 6.1:-74317137 MS.gene052399:CDS
GGGTATGACTTGGGTACCTA+TGG 0.471685 6.1:-74317024 MS.gene052399:CDS
GTTCAGAAATATGAGAAGAA+TGG 0.474815 6.1:-74317477 MS.gene052399:CDS
ACTTACGTAGTTTGGGCCAT+AGG 0.489087 6.1:+74317008 None:intergenic
GTCTATGGACGGCACTATGT+TGG 0.493394 6.1:+74317515 None:intergenic
GATGGATCCTCCATTATACT+TGG 0.494543 6.1:-74315803 MS.gene052399:CDS
ATACAGGGAGTGACTTAACT+TGG 0.496915 6.1:-74317966 MS.gene052399:CDS
TGTAAACAAGAATATTGGTT+GGG 0.497227 6.1:-74315041 MS.gene052399:CDS
AGTGGCAATGTTTATCCAGA+TGG 0.500980 6.1:-74319138 MS.gene052399:CDS
GCCTTATTGGGGTATGACTT+GGG 0.505772 6.1:-74317033 MS.gene052399:CDS
GAACTCCTAAGGAAGAACTA+TGG 0.511046 6.1:+74317406 None:intergenic
GCTTAAAGAAGTTTCTGGGT+TGG 0.511973 6.1:-74316360 MS.gene052399:CDS
GCCAAGCAAAATATTGAAGA+AGG 0.512954 6.1:-74314999 MS.gene052399:CDS
GGAAATGATGGACTTGGCGG+TGG 0.514962 6.1:-74317083 MS.gene052399:CDS
TGGACGGCACTATGTTGGAT+AGG 0.517958 6.1:+74317520 None:intergenic
GGCACTATGTTGGATAGGGT+TGG 0.521370 6.1:+74317525 None:intergenic
GTCATAGCATACTATCACTT+AGG 0.531247 6.1:+74314966 None:intergenic
GGTCATTGCTTAGGAAATGA+TGG 0.531885 6.1:-74317095 MS.gene052399:CDS
ATCCTTAATAAGAACTCCTA+AGG 0.538839 6.1:+74317395 None:intergenic
CATGTACATTGCTACCATCA+AGG 0.542258 6.1:+74315825 None:intergenic
TCACAGAAGCAACAAGATCC+AGG 0.544522 6.1:+74316462 None:intergenic
GAGATTCTTGGAATAAACTA+CGG 0.544989 6.1:-74316578 MS.gene052399:CDS
GAATTTCATAGTCACACTGT+TGG 0.552253 6.1:+74317439 None:intergenic
GGATCCTCCATTATACTTGG+AGG 0.553808 6.1:-74315800 MS.gene052399:intron
ACCTTTGCACAACTTCTGCA+CGG 0.572104 6.1:+74317928 None:intergenic
GGACTGAGCAGAGCGAAAGT+TGG 0.573902 6.1:-74317164 MS.gene052399:CDS
CAGATCTGTCAAAGATGTCA+AGG 0.575041 6.1:-74316058 MS.gene052399:CDS
CACATTGTTTCGGATACCGC+CGG 0.575499 6.1:-74315983 MS.gene052399:CDS
GGACGGCACTATGTTGGATA+GGG 0.579149 6.1:+74317521 None:intergenic
ACTTCTATGCTGCAGAACAA+AGG 0.581078 6.1:-74315866 MS.gene052399:intron
GTGGCAATGTTTATCCAGAT+GGG 0.582598 6.1:-74319137 MS.gene052399:intron
TTAGGAAATGATGGACTTGG+CGG 0.588299 6.1:-74317086 MS.gene052399:CDS
TATTACACGCATTTACGTGT+CGG 0.598280 6.1:-74318021 MS.gene052399:CDS
ATGTAAACAAGAATATTGGT+TGG 0.601433 6.1:-74315042 MS.gene052399:CDS
GTCCATAGACTCTCTATGCG+TGG 0.602803 6.1:-74317502 MS.gene052399:CDS
GGGATTTGAAGCTTAAATTG+GGG 0.604814 6.1:-74319248 MS.gene052399:CDS
CTAATGATCAAGTAACCCTC+CGG 0.605725 6.1:+74315964 None:intergenic
AAACAGCAAAGTTGGAAAGG+TGG 0.619187 6.1:-74316531 MS.gene052399:CDS
TGGAAACAGCAAAGTTGGAA+AGG 0.621929 6.1:-74316534 MS.gene052399:CDS
TTGTTTCGGATACCGCCGGA+GGG 0.622458 6.1:-74315979 MS.gene052399:CDS
ATTGTTTCGGATACCGCCGG+AGG 0.628153 6.1:-74315980 MS.gene052399:CDS
GATGGTAGCAATGTACATGA+TGG 0.643852 6.1:-74315821 MS.gene052399:CDS
AGTACCTCCAAGTATAATGG+AGG 0.647121 6.1:+74315796 None:intergenic
CGGCGGTATCCGAAACAATG+TGG 0.662699 6.1:+74315984 None:intergenic
AAGGTGTGGATATGATCAAG+AGG 0.664812 6.1:-74317228 MS.gene052399:CDS
TCCGTGCAGAAGTTGTGCAA+AGG 0.670250 6.1:-74317929 MS.gene052399:intron
ACGCATAGAGAGTCTATGGA+CGG 0.675187 6.1:+74317504 None:intergenic
AGGTGTGGATATGATCAAGA+GGG 0.680410 6.1:-74317227 MS.gene052399:CDS
GGTTGGGAGAGAACAAAATG+TGG 0.685023 6.1:-74315025 MS.gene052399:CDS
TACTTGCTAACTGATAAGGT+AGG 0.706815 6.1:+74317141 None:intergenic
ACAACATTGCTTGAACTACG+TGG 0.709275 6.1:-74319351 MS.gene052399:intron
TTTGCAGACATTTCACTGCG+AGG 0.713408 6.1:-74315085 MS.gene052399:intron
ATGATCAAGTAACCCTCCGG+CGG 0.715949 6.1:+74315967 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTATTATTATTTGG+AGG - chr6.1:74319106-74319125 MS.gene052399:intron 10.0%
!!! TTCCAATTATTTTTAAATTA+CGG - chr6.1:74315795-74315814 MS.gene052399:intron 10.0%
!! ATCCGTAATTTAAAAATAAT+TGG + chr6.1:74315800-74315819 None:intergenic 15.0%
!! TAAAAATAATTGGAAAATCT+TGG + chr6.1:74315790-74315809 None:intergenic 15.0%
!! TTATATATATTCTTGTGTAA+AGG - chr6.1:74316525-74316544 MS.gene052399:CDS 15.0%
!! AAAAGTTGTATTAGAAAAAG+TGG + chr6.1:74318406-74318425 None:intergenic 20.0%
!! AATGAATTCATATTCAACAT+TGG + chr6.1:74317941-74317960 None:intergenic 20.0%
!! AATGCAATTGAATATTTGAA+GGG - chr6.1:74315938-74315957 MS.gene052399:intron 20.0%
!! AGATTGTTGATGTTAAAAAA+AGG - chr6.1:74315114-74315133 MS.gene052399:intron 20.0%
!! ATCAAACTTTACTTATCTTT+CGG - chr6.1:74318905-74318924 MS.gene052399:intron 20.0%
!! ATTGAAACATTATGTTAACT+CGG - chr6.1:74316753-74316772 MS.gene052399:intron 20.0%
!! CTTAATATAGATCAAGTAAA+AGG - chr6.1:74315257-74315276 MS.gene052399:intron 20.0%
!! TGATCTAAATATTATTGAGT+TGG - chr6.1:74315579-74315598 MS.gene052399:intron 20.0%
!! TTCAAAGAACAAAAATCATT+TGG + chr6.1:74318439-74318458 None:intergenic 20.0%
!! TTGAGAAATCTACAATATAT+CGG - chr6.1:74317410-74317429 MS.gene052399:CDS 20.0%
!!! AAATGATTTTTGTTCTTTGA+AGG - chr6.1:74318438-74318457 MS.gene052399:intron 20.0%
!!! AACAAAATTTAACTTGGTTT+TGG + chr6.1:74316997-74317016 None:intergenic 20.0%
!!! AATACAAGTTTTATAACTCT+AGG - chr6.1:74316097-74316116 MS.gene052399:intron 20.0%
!!! AATGATTTTTGTTCTTTGAA+GGG - chr6.1:74318439-74318458 MS.gene052399:intron 20.0%
!!! ACCAAGTTAAATTTTGTTTT+CGG - chr6.1:74316999-74317018 MS.gene052399:intron 20.0%
!!! ATGCTTTATACACTTTTTTT+TGG - chr6.1:74318657-74318676 MS.gene052399:intron 20.0%
!!! TTATGTCTCTAAATTTTTCT+AGG - chr6.1:74318082-74318101 MS.gene052399:intron 20.0%
!!! TTTCCACTTTTTAAGAAATA+TGG - chr6.1:74315053-74315072 MS.gene052399:CDS 20.0%
! ACACCATATTTCTTAAAAAG+TGG + chr6.1:74315059-74315078 None:intergenic 25.0%
! ATACTCCAACTATATGATAT+AGG - chr6.1:74316209-74316228 MS.gene052399:intron 25.0%
! ATATCTGTGAAAATGAAAAC+AGG - chr6.1:74317485-74317504 MS.gene052399:CDS 25.0%
! ATGTAAACAAGAATATTGGT+TGG - chr6.1:74319296-74319315 MS.gene052399:intron 25.0%
! CAATGCAATTGAATATTTGA+AGG - chr6.1:74315937-74315956 MS.gene052399:intron 25.0%
! GAACAAAAATCATTTGGATA+TGG + chr6.1:74318433-74318452 None:intergenic 25.0%
! GACAATGTAAACAAGAATAT+TGG - chr6.1:74319292-74319311 MS.gene052399:intron 25.0%
! GAGAATTCTAAATGAAAATG+TGG - chr6.1:74319025-74319044 MS.gene052399:intron 25.0%
! GTTGATATATTCAGAGAATA+TGG + chr6.1:74318844-74318863 None:intergenic 25.0%
! TAGTTCCTATATCATATAGT+TGG + chr6.1:74316217-74316236 None:intergenic 25.0%
! TATAAAGCTATAGTAATGTG+CGG + chr6.1:74316597-74316616 None:intergenic 25.0%
! TATTCTCAAATTGGAGATTT+AGG + chr6.1:74317531-74317550 None:intergenic 25.0%
! TCAAAAACTGTACTGTAATT+AGG - chr6.1:74315909-74315928 MS.gene052399:intron 25.0%
! TCATCAACATAAAAAAACCT+TGG + chr6.1:74318620-74318639 None:intergenic 25.0%
! TCTATAAGAAAACTGAGATT+GGG - chr6.1:74315705-74315724 MS.gene052399:intron 25.0%
! TCTCATAAAATTCACTTACA+TGG + chr6.1:74316734-74316753 None:intergenic 25.0%
! TTATCAAAACAAAGAAAAGC+TGG - chr6.1:74318233-74318252 MS.gene052399:intron 25.0%
! TTGAAACATTATGTTAACTC+GGG - chr6.1:74316754-74316773 MS.gene052399:intron 25.0%
! TTTAAGAAATATGGTGTTCT+TGG - chr6.1:74315062-74315081 MS.gene052399:CDS 25.0%
! TTTATGATAGAACATTGTAG+CGG - chr6.1:74315614-74315633 MS.gene052399:intron 25.0%
! TTTATGCATAAGCACTTATA+TGG - chr6.1:74318175-74318194 MS.gene052399:intron 25.0%
!! AAAACGATATTTTGTTGATG+TGG - chr6.1:74316349-74316368 MS.gene052399:CDS 25.0%
!! ACATCAACAAAATATCGTTT+TGG + chr6.1:74316350-74316369 None:intergenic 25.0%
!! AGTTATTTCACATTAAGCTT+TGG - chr6.1:74318767-74318786 MS.gene052399:intron 25.0%
!! CATAATAAAGTTTTCCTTGA+GGG + chr6.1:74318574-74318593 None:intergenic 25.0%
!! TCATAATAAAGTTTTCCTTG+AGG + chr6.1:74318575-74318594 None:intergenic 25.0%
!! TGTAAACAAGAATATTGGTT+GGG - chr6.1:74319297-74319316 MS.gene052399:intron 25.0%
!! TTTTAAATTACGGATACAGA+TGG - chr6.1:74315805-74315824 MS.gene052399:CDS 25.0%
!!! ATAGTTTTAAGAATCAGTGT+TGG + chr6.1:74317554-74317573 None:intergenic 25.0%
!!! ATTCAAGTTGACTAATTTGA+CGG - chr6.1:74315343-74315362 MS.gene052399:intron 25.0%
!!! CCAAGTTAAATTTTGTTTTC+GGG - chr6.1:74317000-74317019 MS.gene052399:intron 25.0%
!!! GGTTTTTTTATGTTGATGAA+TGG - chr6.1:74318621-74318640 MS.gene052399:intron 25.0%
!!! TACTATAGCTTTATAGAATC+AGG - chr6.1:74316602-74316621 MS.gene052399:intron 25.0%
AAAATCCTACTTACGTAGTT+TGG + chr6.1:74317341-74317360 None:intergenic 30.0%
AAATCCTACTTACGTAGTTT+GGG + chr6.1:74317340-74317359 None:intergenic 30.0%
AACCTTGGATAAAAACTAGA+AGG + chr6.1:74318605-74318624 None:intergenic 30.0%
ACTCGGGATATAATTGATAT+AGG - chr6.1:74316770-74316789 MS.gene052399:intron 30.0%
AGAACCTCAATAACACTTAA+TGG + chr6.1:74318717-74318736 None:intergenic 30.0%
ATCCTTAATAAGAACTCCTA+AGG + chr6.1:74316946-74316965 None:intergenic 30.0%
ATTCTTGTGTAAAGGTAAAG+TGG - chr6.1:74316533-74316552 MS.gene052399:CDS 30.0%
CAATGATGCATATGTAATAC+AGG + chr6.1:74316262-74316281 None:intergenic 30.0%
CCCGAAAACAAAATTTAACT+TGG + chr6.1:74317003-74317022 None:intergenic 30.0%
CTCGGGATATAATTGATATA+GGG - chr6.1:74316771-74316790 MS.gene052399:intron 30.0%
CTTATCAGTTAGCAAGTAAA+GGG - chr6.1:74317202-74317221 MS.gene052399:CDS 30.0%
CTTCATCTTATGACTACTAA+TGG - chr6.1:74316969-74316988 MS.gene052399:intron 30.0%
GAGATTCTTGGAATAAACTA+CGG - chr6.1:74317760-74317779 MS.gene052399:intron 30.0%
GCAATATCATGAATTCTCAT+TGG - chr6.1:74315837-74315856 MS.gene052399:CDS 30.0%
GCATATGTAATACAGGAAAT+TGG + chr6.1:74316255-74316274 None:intergenic 30.0%
GTCTATAAGAAAACTGAGAT+TGG - chr6.1:74315704-74315723 MS.gene052399:intron 30.0%
GTTCAGAAATATGAGAAGAA+TGG - chr6.1:74316861-74316880 MS.gene052399:intron 30.0%
TCTTATATTTAAGGACTAGG+TGG - chr6.1:74316004-74316023 MS.gene052399:CDS 30.0%
TGTTCATAAATAACTCAGCT+AGG - chr6.1:74318685-74318704 MS.gene052399:intron 30.0%
TTACATATGCATCATTGTGT+TGG - chr6.1:74316265-74316284 MS.gene052399:intron 30.0%
TTATACCAAACTGAGATTCT+TGG - chr6.1:74317748-74317767 MS.gene052399:intron 30.0%
TTATGATAGAACATTGTAGC+GGG - chr6.1:74315615-74315634 MS.gene052399:intron 30.0%
TTATTCCAAGAATCTCAGTT+TGG + chr6.1:74317756-74317775 None:intergenic 30.0%
TTCCTTAGGAGTTCTTATTA+AGG - chr6.1:74316941-74316960 MS.gene052399:intron 30.0%
! AAGCCTTATCTCACTAAATA+GGG + chr6.1:74315660-74315679 None:intergenic 30.0%
! AGGGATTTGAAGCTTAAATT+GGG - chr6.1:74315089-74315108 MS.gene052399:intron 30.0%
! ATTTTGTTGATGTGGATACA+GGG - chr6.1:74316357-74316376 MS.gene052399:CDS 30.0%
! CTCAGTTTTCTTATAGACAA+TGG + chr6.1:74315702-74315721 None:intergenic 30.0%
! GTTTCTCTGATAGTTTTTCT+AGG + chr6.1:74315550-74315569 None:intergenic 30.0%
! TAGACCATTAAGTGTTATTG+AGG - chr6.1:74318710-74318729 MS.gene052399:intron 30.0%
! TATTTTGTTGATGTGGATAC+AGG - chr6.1:74316356-74316375 MS.gene052399:CDS 30.0%
! TCAACAAAATATCGTTTTGG+AGG + chr6.1:74316347-74316366 None:intergenic 30.0%
! TTATACGTATTTTCCAAGAG+AGG - chr6.1:74317849-74317868 MS.gene052399:intron 30.0%
!! CAACAAAATATCGTTTTGGA+GGG + chr6.1:74316346-74316365 None:intergenic 30.0%
!! GAACATTTTGAAAACACTTG+AGG - chr6.1:74319055-74319074 MS.gene052399:intron 30.0%
!! TATTGAGTTGGATCGAAATA+TGG - chr6.1:74315591-74315610 MS.gene052399:intron 30.0%
!! TGATGATTTTGTGCCTTATT+GGG - chr6.1:74317293-74317312 MS.gene052399:intron 30.0%
!! TTCAAGTTGACTAATTTGAC+GGG - chr6.1:74315344-74315363 MS.gene052399:intron 30.0%
!! TTGAAGGGTGTCTTATAAAA+GGG - chr6.1:74315953-74315972 MS.gene052399:intron 30.0%
!! TTTGAAGGGTGTCTTATAAA+AGG - chr6.1:74315952-74315971 MS.gene052399:intron 30.0%
!!! ACGAATTTTTTTTTGCGAGA+GGG - chr6.1:74315981-74316000 MS.gene052399:CDS 30.0%
!!! GGCAAGTTTTTGTTTTGAAT+TGG - chr6.1:74316173-74316192 MS.gene052399:intron 30.0%
!!! GTTTTATAACTCTAGGATTG+AGG - chr6.1:74316104-74316123 MS.gene052399:intron 30.0%
AACACATTGGCAAAAACAGA+TGG - chr6.1:74317144-74317163 MS.gene052399:CDS 35.0%
AACATACACATCGTGTAGAA+TGG + chr6.1:74317088-74317107 None:intergenic 35.0%
AATGTTGTTGGTCATTGCTT+AGG - chr6.1:74317234-74317253 MS.gene052399:intron 35.0%
ACACAACTTTGCCAATTTGT+TGG - chr6.1:74318022-74318041 MS.gene052399:CDS 35.0%
AGATCTGCATGCATATAACA+TGG + chr6.1:74318267-74318286 None:intergenic 35.0%
AGCACCTATAGTAGAATAGA+CGG - chr6.1:74315406-74315425 MS.gene052399:intron 35.0%
ATAGGTTCAAATATCCTCCA+TGG - chr6.1:74316135-74316154 MS.gene052399:intron 35.0%
ATGCAGCTTAAAGAAGTTTC+TGG - chr6.1:74317973-74317992 MS.gene052399:CDS 35.0%
CAACATTAGAGAGACAATTG+AGG - chr6.1:74315382-74315401 MS.gene052399:intron 35.0%
CATGAATTCTCATTGGATGT+CGG - chr6.1:74315844-74315863 MS.gene052399:CDS 35.0%
CCTTATCAGTTAGCAAGTAA+AGG - chr6.1:74317201-74317220 MS.gene052399:CDS 35.0%
CTCCATGGTAAACAAAGAAA+GGG - chr6.1:74316150-74316169 MS.gene052399:intron 35.0%
CTTATCTCACTAAATAGGGT+CGG + chr6.1:74315656-74315675 None:intergenic 35.0%
GAATTTCATAGTCACACTGT+TGG + chr6.1:74316902-74316921 None:intergenic 35.0%
GCAAAAACAGATGGAATCAT+GGG - chr6.1:74317153-74317172 MS.gene052399:CDS 35.0%
GCCAAGCAAAATATTGAAGA+AGG - chr6.1:74319339-74319358 MS.gene052399:intron 35.0%
GGGCTTATTAAGAATGTTGT+TGG - chr6.1:74317222-74317241 MS.gene052399:CDS 35.0%
TACACGATGTGTATGTTGTA+AGG - chr6.1:74317091-74317110 MS.gene052399:CDS 35.0%
TATTACACGCATTTACGTGT+CGG - chr6.1:74316317-74316336 MS.gene052399:CDS 35.0%
TCAGATGACTCAGATCATTA+GGG - chr6.1:74315513-74315532 MS.gene052399:intron 35.0%
TCCATGGTAAACAAAGAAAG+GGG - chr6.1:74316151-74316170 MS.gene052399:intron 35.0%
TGCAGCTTAAAGAAGTTTCT+GGG - chr6.1:74317974-74317993 MS.gene052399:CDS 35.0%
TTTGATGGAAACAGCAAAGT+TGG - chr6.1:74317799-74317818 MS.gene052399:intron 35.0%
! AAATTAGCCTGCCAACAAAT+TGG + chr6.1:74318036-74318055 None:intergenic 35.0%
! AAGACATTAACGCTACGTTT+TGG - chr6.1:74318311-74318330 MS.gene052399:intron 35.0%
! AGTGAAAGCATAGTGAGTAA+TGG + chr6.1:74316488-74316507 None:intergenic 35.0%
! AGTTGGAGTATCTTAGTTCT+AGG + chr6.1:74316200-74316219 None:intergenic 35.0%
! CCTTTACTTGCTAACTGATA+AGG + chr6.1:74317204-74317223 None:intergenic 35.0%
! CGATTTTTAACATACCCATC+TGG + chr6.1:74315218-74315237 None:intergenic 35.0%
! CTTAAAGAAGTTTCTGGGTT+GGG - chr6.1:74317979-74317998 MS.gene052399:CDS 35.0%
! GAAGCCTTATCTCACTAAAT+AGG + chr6.1:74315661-74315680 None:intergenic 35.0%
! GAGGGATTTGAAGCTTAAAT+TGG - chr6.1:74315088-74315107 MS.gene052399:intron 35.0%
! GCCTTCTTCAATATTTTGCT+TGG + chr6.1:74319343-74319362 None:intergenic 35.0%
! GGATTTTGTCCACATTGTTT+CGG - chr6.1:74318345-74318364 MS.gene052399:intron 35.0%
! GGGATTTGAAGCTTAAATTG+GGG - chr6.1:74315090-74315109 MS.gene052399:intron 35.0%
! GGGTCTTATATTTAAGGACT+AGG - chr6.1:74316001-74316020 MS.gene052399:CDS 35.0%
! GTGATGATTTTGTGCCTTAT+TGG - chr6.1:74317292-74317311 MS.gene052399:intron 35.0%
! TACTTGCTAACTGATAAGGT+AGG + chr6.1:74317200-74317219 None:intergenic 35.0%
! TTAAAGAAGTTTCTGGGTTG+GGG - chr6.1:74317980-74317999 MS.gene052399:CDS 35.0%
!! AATCGTCTTCTCAGTTTTGA+TGG - chr6.1:74317784-74317803 MS.gene052399:intron 35.0%
!! CAGTGTTGGTATTCTCAAAT+TGG + chr6.1:74317540-74317559 None:intergenic 35.0%
!! CTTGTTATTTGTTGTTGCTG+AGG - chr6.1:74317669-74317688 MS.gene052399:intron 35.0%
!! CTTTATTGCTTCTAGCTCTA+AGG - chr6.1:74315139-74315158 MS.gene052399:intron 35.0%
!! GAAAGTACCTCCAAGTATAA+TGG + chr6.1:74318548-74318567 None:intergenic 35.0%
!! GATGATTTTGTGCCTTATTG+GGG - chr6.1:74317294-74317313 MS.gene052399:intron 35.0%
!! TGAAGGGTGTCTTATAAAAG+GGG - chr6.1:74315954-74315973 MS.gene052399:intron 35.0%
!!! CACGAATTTTTTTTTGCGAG+AGG - chr6.1:74315980-74315999 MS.gene052399:CDS 35.0%
!!! GTGCTGTCTTTTTTCTCTTA+AGG - chr6.1:74317037-74317056 MS.gene052399:CDS 35.0%
!!! TACGTTTTGGAAACAAATGG+TGG - chr6.1:74318324-74318343 MS.gene052399:intron 35.0%
!!! TGCCTTCTAGTTTTTATCCA+AGG - chr6.1:74318600-74318619 MS.gene052399:intron 35.0%
AAAACTGAGATTGGGTCACT+CGG - chr6.1:74315713-74315732 MS.gene052399:intron 40.0%
AAACAGCAAAGTTGGAAAGG+TGG - chr6.1:74317807-74317826 MS.gene052399:intron 40.0%
AAGGTGTGGATATGATCAAG+AGG - chr6.1:74317110-74317129 MS.gene052399:CDS 40.0%
AAGTGCTCCAAACAGAATGT+TGG + chr6.1:74317652-74317671 None:intergenic 40.0%
AATATGGTGTTCTTGGTCAG+AGG - chr6.1:74315069-74315088 MS.gene052399:CDS 40.0%
ACAACATTGCTTGAACTACG+TGG - chr6.1:74314987-74315006 MS.gene052399:CDS 40.0%
ACCTATAGTAGAATAGACGG+TGG - chr6.1:74315409-74315428 MS.gene052399:intron 40.0%
ACTTCTATGCTGCAGAACAA+AGG - chr6.1:74318472-74318491 MS.gene052399:intron 40.0%
AGGATTGAGGTCATACTCAT+AGG - chr6.1:74316117-74316136 MS.gene052399:intron 40.0%
AGGTGTGGATATGATCAAGA+GGG - chr6.1:74317111-74317130 MS.gene052399:CDS 40.0%
AGTAAGGAGAGTAGATCAGA+TGG - chr6.1:74315483-74315502 MS.gene052399:intron 40.0%
AGTACCTCCAAGTATAATGG+AGG + chr6.1:74318545-74318564 None:intergenic 40.0%
AGTGGAAACAGAAAGCTACT+TGG + chr6.1:74315041-74315060 None:intergenic 40.0%
AGTGGCAATGTTTATCCAGA+TGG - chr6.1:74315200-74315219 MS.gene052399:intron 40.0%
ATACAGGGAGTGACTTAACT+TGG - chr6.1:74316372-74316391 MS.gene052399:intron 40.0%
ATACTGATGCACTACTGTGT+AGG + chr6.1:74316076-74316095 None:intergenic 40.0%
ATATGGTGTTCTTGGTCAGA+GGG - chr6.1:74315070-74315089 MS.gene052399:CDS 40.0%
CAGATCTGTCAAAGATGTCA+AGG - chr6.1:74318280-74318299 MS.gene052399:intron 40.0%
CAGATGACTCAGATCATTAG+GGG - chr6.1:74315514-74315533 MS.gene052399:intron 40.0%
CAGGAAACTGATGAAGTAGT+CGG - chr6.1:74317504-74317523 MS.gene052399:CDS 40.0%
CATGTACATTGCTACCATCA+AGG + chr6.1:74318516-74318535 None:intergenic 40.0%
CCATGGTAAACAAAGAAAGG+GGG - chr6.1:74316152-74316171 MS.gene052399:intron 40.0%
CCTCCATGGTAAACAAAGAA+AGG - chr6.1:74316149-74316168 MS.gene052399:intron 40.0%
CGACCCTATTTAGTGAGATA+AGG - chr6.1:74315654-74315673 MS.gene052399:intron 40.0%
CTAATGATCAAGTAACCCTC+CGG + chr6.1:74318377-74318396 None:intergenic 40.0%
CTAATTTCCGGATCAGGTAA+GGG - chr6.1:74318049-74318068 MS.gene052399:intron 40.0%
CTCCTTACTGCATAATCTCT+GGG + chr6.1:74315472-74315491 None:intergenic 40.0%
GAAACTGAGCTTAAGTGGTT+TGG - chr6.1:74315431-74315450 MS.gene052399:intron 40.0%
GAACTCCTAAGGAAGAACTA+TGG + chr6.1:74316935-74316954 None:intergenic 40.0%
GATGGATCCTCCATTATACT+TGG - chr6.1:74318535-74318554 MS.gene052399:intron 40.0%
GATGGTAGCAATGTACATGA+TGG - chr6.1:74318517-74318536 MS.gene052399:intron 40.0%
GATGTGTATGTTGTAAGGTG+TGG - chr6.1:74317096-74317115 MS.gene052399:CDS 40.0%
GCATAGTGAGTAATGGTTTC+TGG + chr6.1:74316481-74316500 None:intergenic 40.0%
GGATAAACATTGCCACTGAT+TGG + chr6.1:74315197-74315216 None:intergenic 40.0%
GGCAAAAACAGATGGAATCA+TGG - chr6.1:74317152-74317171 MS.gene052399:CDS 40.0%
GGTCATTGCTTAGGAAATGA+TGG - chr6.1:74317243-74317262 MS.gene052399:intron 40.0%
GTAAAGGTAAAGTGGAGCTT+TGG - chr6.1:74316541-74316560 MS.gene052399:CDS 40.0%
GTCAGATGACTCAGATCATT+AGG - chr6.1:74315512-74315531 MS.gene052399:intron 40.0%
GTGAATGCCAACATTCTGTT+TGG - chr6.1:74317642-74317661 MS.gene052399:intron 40.0%
GTGGCAATGTTTATCCAGAT+GGG - chr6.1:74315201-74315220 MS.gene052399:intron 40.0%
GTTTGCTTAGCTATCCTTGA+TGG - chr6.1:74318499-74318518 MS.gene052399:intron 40.0%
TCCACCGTCTATTCTACTAT+AGG + chr6.1:74315413-74315432 None:intergenic 40.0%
TCTCCTTACTGCATAATCTC+TGG + chr6.1:74315473-74315492 None:intergenic 40.0%
TCTTGGATTCCAATCAGACT+CGG + chr6.1:74315773-74315792 None:intergenic 40.0%
TGGAAACAGCAAAGTTGGAA+AGG - chr6.1:74317804-74317823 MS.gene052399:intron 40.0%
TTACAGTGCAAATAGCTGAG+AGG + chr6.1:74316682-74316701 None:intergenic 40.0%
TTTGTTGGCAGGCTAATTTC+CGG - chr6.1:74318037-74318056 MS.gene052399:intron 40.0%
! AGTTTTACACCGAGTCTGAT+TGG - chr6.1:74315761-74315780 MS.gene052399:intron 40.0%
! CCTTTCTTTGTTTACCATGG+AGG + chr6.1:74316152-74316171 None:intergenic 40.0%
! GCGAGAGGGTCTTATATTTA+AGG - chr6.1:74315995-74316014 MS.gene052399:CDS 40.0%
! GCTTAAAGAAGTTTCTGGGT+TGG - chr6.1:74317978-74317997 MS.gene052399:CDS 40.0%
! GGAAAGGTGGTTTTTGATAG+TGG - chr6.1:74317820-74317839 MS.gene052399:intron 40.0%
!! AACTTTGCCAATTTGTTGGC+AGG - chr6.1:74318026-74318045 MS.gene052399:CDS 40.0%
!! AAGGGTGTCTTATAAAAGGG+GGG - chr6.1:74315956-74315975 MS.gene052399:intron 40.0%
!! GAAGGGTGTCTTATAAAAGG+GGG - chr6.1:74315955-74315974 MS.gene052399:intron 40.0%
!! GGATAGGGTTGGCTTATATA+TGG + chr6.1:74316805-74316824 None:intergenic 40.0%
!! TGCTTAGGAAATGATGGACT+TGG - chr6.1:74317249-74317268 MS.gene052399:intron 40.0%
!! TTAGGAAATGATGGACTTGG+CGG - chr6.1:74317252-74317271 MS.gene052399:intron 40.0%
!!! CGCTACGTTTTGGAAACAAA+TGG - chr6.1:74318321-74318340 MS.gene052399:intron 40.0%
!!! TCTGCTGTTTTTCCAATCAG+TGG - chr6.1:74315182-74315201 MS.gene052399:intron 40.0%
AAACTGAGATTGGGTCACTC+GGG - chr6.1:74315714-74315733 MS.gene052399:intron 45.0%
AAGGAGAGTAGATCAGATGG+AGG - chr6.1:74315486-74315505 MS.gene052399:intron 45.0%
ACCCAAGTCATACCCCAATA+AGG + chr6.1:74317309-74317328 None:intergenic 45.0%
ACCTTTGCACAACTTCTGCA+CGG + chr6.1:74316413-74316432 None:intergenic 45.0%
ACGCATAGAGAGTCTATGGA+CGG + chr6.1:74316837-74316856 None:intergenic 45.0%
ACTCAGATCATTAGGGGTAG+AGG - chr6.1:74315520-74315539 MS.gene052399:intron 45.0%
ACTTACGTAGTTTGGGCCAT+AGG + chr6.1:74317333-74317352 None:intergenic 45.0%
AGCCCAGAGATTATGCAGTA+AGG - chr6.1:74315467-74315486 MS.gene052399:intron 45.0%
ATGGCCCAAACTACGTAAGT+AGG - chr6.1:74317333-74317352 MS.gene052399:intron 45.0%
CATTTCACTGCGAGGACATT+TGG - chr6.1:74319261-74319280 MS.gene052399:intron 45.0%
CGTGGTCTCAAGAACAATGA+TGG - chr6.1:74315005-74315024 MS.gene052399:CDS 45.0%
GCAGACCATAGTTCTTCCTT+AGG - chr6.1:74316927-74316946 MS.gene052399:intron 45.0%
GCTAATTTCCGGATCAGGTA+AGG - chr6.1:74318048-74318067 MS.gene052399:intron 45.0%
GGATCCTCCATTATACTTGG+AGG - chr6.1:74318538-74318557 MS.gene052399:intron 45.0%
GGTTGGGAGAGAACAAAATG+TGG - chr6.1:74319313-74319332 MS.gene052399:intron 45.0%
GTGATTTCCCTTACCTGATC+CGG + chr6.1:74318059-74318078 None:intergenic 45.0%
TAATCCATCTCAACACGCAG+AGG + chr6.1:74316454-74316473 None:intergenic 45.0%
TCACAGAAGCAACAAGATCC+AGG + chr6.1:74317879-74317898 None:intergenic 45.0%
TCGGGGCATACACATTATCA+GGG - chr6.1:74315732-74315751 MS.gene052399:intron 45.0%
TGCGTGTAATACAACCTGCA+CGG + chr6.1:74316308-74316327 None:intergenic 45.0%
TTCCACGCATAGAGAGTCTA+TGG + chr6.1:74316841-74316860 None:intergenic 45.0%
TTTGCAGACATTTCACTGCG+AGG - chr6.1:74319253-74319272 MS.gene052399:intron 45.0%
! CCCCCTTTCTTTGTTTACCA+TGG + chr6.1:74316155-74316174 None:intergenic 45.0%
! GCCTTATTGGGGTATGACTT+GGG - chr6.1:74317305-74317324 MS.gene052399:intron 45.0%
! GGGCATGCTTTTGAACACAT+TGG - chr6.1:74317131-74317150 MS.gene052399:CDS 45.0%
! TGCCTTATTGGGGTATGACT+TGG - chr6.1:74317304-74317323 MS.gene052399:intron 45.0%
!! TTTTTCGGAGAAGAGGATGC+TGG + chr6.1:74318944-74318963 None:intergenic 45.0%
!! GAATTATTATTATTATTATT+TGG - chr6.1:74319103-74319122 MS.gene052399:intron 5.0%
AACTGAGATTGGGTCACTCG+GGG - chr6.1:74315715-74315734 MS.gene052399:intron 50.0%
AGCAACATGTTCTTCCGTGC+AGG - chr6.1:74316291-74316310 MS.gene052399:intron 50.0%
ATGATCAAGTAACCCTCCGG+CGG + chr6.1:74318374-74318393 None:intergenic 50.0%
CACATTGTTTCGGATACCGC+CGG - chr6.1:74318355-74318374 MS.gene052399:intron 50.0%
CGGTGGAAACTGAGCTTAAG+TGG - chr6.1:74315426-74315445 MS.gene052399:intron 50.0%
CTCGGGGCATACACATTATC+AGG - chr6.1:74315731-74315750 MS.gene052399:intron 50.0%
GGACGGCACTATGTTGGATA+GGG + chr6.1:74316820-74316839 None:intergenic 50.0%
GGCACTATGTTGGATAGGGT+TGG + chr6.1:74316816-74316835 None:intergenic 50.0%
GGCGGTGGATACATGTTCTT+AGG - chr6.1:74317270-74317289 MS.gene052399:intron 50.0%
GTCCATAGACTCTCTATGCG+TGG - chr6.1:74316836-74316855 MS.gene052399:intron 50.0%
GTCTATGGACGGCACTATGT+TGG + chr6.1:74316826-74316845 None:intergenic 50.0%
GTTACCTCTGCGTGTTGAGA+TGG - chr6.1:74316447-74316466 MS.gene052399:intron 50.0%
TCCGTGCAGAAGTTGTGCAA+AGG - chr6.1:74316409-74316428 MS.gene052399:intron 50.0%
TGGACGGCACTATGTTGGAT+AGG + chr6.1:74316821-74316840 None:intergenic 50.0%
! CGGATACAGATGGACTTACG+TGG - chr6.1:74315815-74315834 MS.gene052399:CDS 50.0%
! GAGGTACTTTCACACCCTCA+AGG - chr6.1:74318557-74318576 MS.gene052399:intron 50.0%
! GGGTATGACTTGGGTACCTA+TGG - chr6.1:74317314-74317333 MS.gene052399:intron 50.0%
!! TTTTCCAAGAGAGGCGTACC+TGG - chr6.1:74317858-74317877 MS.gene052399:intron 50.0%
AGATCCAGGTACGCCTCTCT+TGG + chr6.1:74317865-74317884 None:intergenic 55.0%
ATTGTTTCGGATACCGCCGG+AGG - chr6.1:74318358-74318377 MS.gene052399:intron 55.0%
CGGCGGTATCCGAAACAATG+TGG + chr6.1:74318357-74318376 None:intergenic 55.0%
GGACTGAGCAGAGCGAAAGT+TGG - chr6.1:74317174-74317193 MS.gene052399:CDS 55.0%
GGCAGGCTAATTTCCGGATC+AGG - chr6.1:74318043-74318062 MS.gene052399:intron 55.0%
TTGTTTCGGATACCGCCGGA+GGG - chr6.1:74318359-74318378 MS.gene052399:intron 55.0%
!! GGAAATGATGGACTTGGCGG+TGG - chr6.1:74317255-74317274 MS.gene052399:intron 55.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 74314967 74319393 74314967 ID=MS.gene052399
chr6.1 mRNA 74314967 74319393 74314967 ID=MS.gene052399.t1;Parent=MS.gene052399
chr6.1 exon 74319352 74319393 74319352 ID=MS.gene052399.t1.exon1;Parent=MS.gene052399.t1
chr6.1 CDS 74319352 74319393 74319352 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74319138 74319271 74319138 ID=MS.gene052399.t1.exon2;Parent=MS.gene052399.t1
chr6.1 CDS 74319138 74319271 74319138 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74317930 74318047 74317930 ID=MS.gene052399.t1.exon3;Parent=MS.gene052399.t1
chr6.1 CDS 74317930 74318047 74317930 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74317339 74317568 74317339 ID=MS.gene052399.t1.exon4;Parent=MS.gene052399.t1
chr6.1 CDS 74317339 74317568 74317339 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74317014 74317247 74317014 ID=MS.gene052399.t1.exon5;Parent=MS.gene052399.t1
chr6.1 CDS 74317014 74317247 74317014 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74316466 74316616 74316466 ID=MS.gene052399.t1.exon6;Parent=MS.gene052399.t1
chr6.1 CDS 74316466 74316616 74316466 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74316296 74316381 74316296 ID=MS.gene052399.t1.exon7;Parent=MS.gene052399.t1
chr6.1 CDS 74316296 74316381 74316296 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74315963 74316077 74315963 ID=MS.gene052399.t1.exon8;Parent=MS.gene052399.t1
chr6.1 CDS 74315963 74316077 74315963 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74315801 74315873 74315801 ID=MS.gene052399.t1.exon9;Parent=MS.gene052399.t1
chr6.1 CDS 74315801 74315873 74315801 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
chr6.1 exon 74314967 74315100 74314967 ID=MS.gene052399.t1.exon10;Parent=MS.gene052399.t1
chr6.1 CDS 74314967 74315100 74314967 ID=cds.MS.gene052399.t1;Parent=MS.gene052399.t1
Gene Sequence

>MS.gene052399

ATGGTTCACTATTTTCAACTACAACATTGCTTGAACTACGTGGTCTCAAGAACAATGATGGTGATGATGAACCAAGTAGCTTTCTGTTTCCACTTTTTAAGAAATATGGTGTTCTTGGTCAGAGGGATTTGAAGCTTAAATTGGGGAAGATTGTTGATGTTAAAAAAAGGAACTTTATTGCTTCTAGCTCTAAGGTTGTAGCTGTTGACTCTTCATCTGCTGTTTTTCCAATCAGTGGCAATGTTTATCCAGATGGGTATGTTAAAAATCGTTGCTTTTTGCTTATTTGACTTAATATAGATCAAGTAAAAGGTTGAAAGTTTTGAACTTTTTCACTTTTTTGTTGTTATCAGATCACGTTGACTAATTGTTGATTATTCAAGTTGACTAATTTGACGGGCTAAATTAGAAATGACAACATTAGAGAGACAATTGAGGTAGCACCTATAGTAGAATAGACGGTGGAAACTGAGCTTAAGTGGTTTGGACATGTAGAGAGAAGCCCAGAGATTATGCAGTAAGGAGAGTAGATCAGATGGAGGATAGTCAGATGACTCAGATCATTAGGGGTAGAGGAAAACCTAGAAAAACTATCAGAGAAACTATTAAGAATGATCTAAATATTATTGAGTTGGATCGAAATATGGTTTATGATAGAACATTGTAGCGGGATTTAATCTATGCAGCCGACCCTATTTAGTGAGATAAGGCTTCATTGTTTTTGTTGTTATTCCATTGTCTATAAGAAAACTGAGATTGGGTCACTCGGGGCATACACATTATCAGGGTAAACTAGTTTTACACCGAGTCTGATTGGAATCCAAGATTTTCCAATTATTTTTAAATTACGGATACAGATGGACTTACGTGGCAATATCATGAATTCTCATTGGATGTCGGTGTAAAACTATTTTACATCAGCTATATAGATCTCTATTAAACTCAAAAACTGTACTGTAATTAGGAGTATCAATGCAATTGAATATTTGAAGGGTGTCTTATAAAAGGGGGGACACGAATTTTTTTTTGCGAGAGGGTCTTATATTTAAGGACTAGGTGGTACGTAAGTTTTTCTGTAGAGTGACATTTGTTCTTTTATGCAGCTACCTACACAGTAGTGCATCAGTATCAATACAAGTTTTATAACTCTAGGATTGAGGTCATACTCATAGGTTCAAATATCCTCCATGGTAAACAAAGAAAGGGGGCAAGTTTTTGTTTTGAATTGGTCCTAGAACTAAGATACTCCAACTATATGATATAGGAACTACTGATTTGTGCTATTCCAATTTCCTGTATTACATATGCATCATTGTGTTGGCTTAGCAACATGTTCTTCCGTGCAGGTTGTATTACACGCATTTACGTGTCGGAAACCCTCCAAAACGATATTTTGTTGATGTGGATACAGGGAGTGACTTAACTTGGATTCAGTGTGATGCTCCGTGCAGAAGTTGTGCAAAGGTAATATCTGACTACTGTTACCTCTGCGTGTTGAGATGGATTATATGCCAGAAACCATTACTCACTATGCTTTCACTACTTATATTTTTAAAAATTATATATATTCTTGTGTAAAGGTAAAGTGGAGCTTTGGCTTAACAGTTTGAGTCGTTATCATGTGATTCCGCACATTACTATAGCTTTATAGAATCAGGCTGCACTGCACTTCACACATTATAGTGTAATCATAAACTGAATATTAGAAAATGCCTCTCAGCTATTTGCACTGTAAATATATGCTGACTATATTTTTTTTTTTTTCCATGTAAGTGAATTTTATGAGATTGAAACATTATGTTAACTCGGGATATAATTGATATAGGGAGCAAATGCCATATATAAGCCAACCCTATCCAACATAGTGCCGTCCATAGACTCTCTATGCGTGGAAGTTCAGAAATATGAGAAGAATGGATATGATGAAAACTTCCAACAGTGTGACTATGAAATTCAATATGCAGACCATAGTTCTTCCTTAGGAGTTCTTATTAAGGATGAGCTTCATCTTATGACTACTAATGGATCCAAAACCAAGTTAAATTTTGTTTTCGGGTAAGAATCATCATTGTGCTGTCTTTTTTCTCTTAAGGCAATATTTTCTACAAAAGTGTTGATCCATTCTACACGATGTGTATGTTGTAAGGTGTGGATATGATCAAGAGGGCATGCTTTTGAACACATTGGCAAAAACAGATGGAATCATGGGACTGAGCAGAGCGAAAGTTGGCCTACCTTATCAGTTAGCAAGTAAAGGGCTTATTAAGAATGTTGTTGGTCATTGCTTAGGAAATGATGGACTTGGCGGTGGATACATGTTCTTAGGTGATGATTTTGTGCCTTATTGGGGTATGACTTGGGTACCTATGGCCCAAACTACGTAAGTAGGATTTTCTTGACAATGTGCTTCTTTACTCTTCATGAAGTGACTTTGTTTCTTCGTTTGAGAAATCTACAATATATCGGTCTGTTTATTTTCTAAACATTTGTCAGTCTTTGTTTTCAATTTTTACTTTGTATATCTGTGAAAATGAAAACAGGAAACTGATGAAGTAGTCGGACCTAAATCTCCAATTTGAGAATACCAACACTGATTCTTAAAACTATTGTTGTATATTGTTTTTGTTTGAGAATTTTTGTTTTTAAATGTTTTTGTAAATCTACTGTTAATTTTTGTGAATGCCAACATTCTGTTTGGAGCACTTGTTATTTGTTGTTGCTGAGGTTTCTTCTGATTCATCTATAATTCATATGTGACATATTCTCTCTCTATACAGAGATTTATACCAAACTGAGATTCTTGGAATAAACTACGGAAATCGTCTTCTCAGTTTTGATGGAAACAGCAAAGTTGGAAAGGTGGTTTTTGATAGTGGCAGTTCTTATACGTATTTTCCAAGAGAGGCGTACCTGGATCTTGTTGCTTCTGTGAGTTTTTACATCTGACGAAAATTATGTACTTGCTTTTAGTCCAATGTTGAATATGAATTCATTATGAATACAATGATGCAGCTTAAAGAAGTTTCTGGGTTGGGGCTCATTCAAGATGATTCAGACACAACTTTGCCAATTTGTTGGCAGGCTAATTTCCGGATCAGGTAAGGGAAATCACTGCTTATGTCTCTAAATTTTTCTAGGATCGCTTACGAAAACAGCTTATAACTTATATGAAAATAGTTTGATTTTAATTTTTTTTTATAAGAAATAGTTTATGCATAAGCACTTATATGGTAAGTGTTTATGCTATAAGCGCTTACTTAAGTTGTTTATCAAAACAAAGAAAAGCTGGTCTAAAATCCATGTTATATGCATGCAGATCTGTCAAAGATGTCAAGGATTATTTCAAGACATTAACGCTACGTTTTGGAAACAAATGGTGGATTTTGTCCACATTGTTTCGGATACCGCCGGAGGGTTACTTGATCATTAGTGTAATCCACTTTTTCTAATACAACTTTTATTTCCATATCCAAATGATTTTTGTTCTTTGAAGGGCTTCTAATAAACTTCTATGCTGCAGAACAAAGGCAATGTTTGCTTAGCTATCCTTGATGGTAGCAATGTACATGATGGATCCTCCATTATACTTGGAGGTACTTTCACACCCTCAAGGAAAACTTTATTATGATATTGTGCCTTCTAGTTTTTATCCAAGGTTTTTTTATGTTGATGAATGGATTTCAAAAAAATATGCTTTATACACTTTTTTTTGGTGTGTTGTTCATAAATAACTCAGCTAGGATTAGACCATTAAGTGTTATTGAGGTTCTGTCACTTTATTATAGTAAATCAAGTTACTAAGTTATTTCACATTAAGCTTTGGAACTTCTATGACTTTTGATTCATATATATTAGCGTTACTAACTTTTGCTATCCATATTCTCTGAATATATCAACTTTTGCTATCTTTGAATGTTGACTAGAATAATCAATCATTGATCAAACTTTACTTATCTTTCGGTTGAACTCTATTACCAGCATCCTCTTCTCCGAAAAACAGGATGGTTAAGATAAAAAAAAAAAAAAAAAAAAAGATACACGTGTACTAACATAGTAAAGAGAATTCTAAATGAAAATGTGGATGCAATGAACATTTTGAAAACACTTGAGGCTTTCTTTTTTTCATTTTGTAATTTGAATTATTATTATTATTATTTGGAGGATTCATAATTATTCAAATTTTTATTTGACAACAAGCTATATTTTTCTTATAAAAATTTTATTCTGCACATCTTATTTTCTAATCTGTGACATTGTTTCTTAATTGATTATGAATAAATTTAATCTTTGCAGACATTTCACTGCGAGGACATTTGGTTGTGTATGACAATGTAAACAAGAATATTGGTTGGGAGAGAACAAAATGTGGCATGCCAAGCAAAATATTGAAGAAGGCTCACAATTTCCTAAGTGATAGTATGCTATGA
Protein sequence

>MS.gene052399.t1

MVHYFQLQHCLNYVRDLKLKLGKIVDVKKRNFIASSSKVVAVDSSSAVFPISGNVYPDGLYYTHLRVGNPPKRYFVDVDTGSDLTWIQCDAPCRSCAKGANAIYKPTLSNIVPSIDSLCVEVQKYEKNGYDENFQQCDYEIQYADHSSSLGVLIKDELHLMTTNGSKTKLNFVFGCGYDQEGMLLNTLAKTDGIMGLSRAKVGLPYQLASKGLIKNVVGHCLGNDGLGGGYMFLGDDFVPYWGMTWVPMAQTTDLYQTEILGINYGNRLLSFDGNSKVGKVVFDSGSSYTYFPREAYLDLVASLKEVSGLGLIQDDSDTTLPICWQANFRIRSVKDVKDYFKTLTLRFGNKWWILSTLFRIPPEGYLIISNKGNVCLAILDGSNVHDGSSIILGDISLRGHLVVYDNVNKNIGWERTKCGMPSKILKKAHNFLSDSML