AlfalfaGEDB | MS.gene052733

M. sativa cultivar XinJiangDaYe / MS.gene052733

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008144 GO:0008150 GO:0009987 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052733.t1	XP_004487053.1	96.3	81	1	1	1	81	345	423	1.10E-33	152.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052733.t1	Q9LZU5	90.1	81	6	1	1	81	340	418	8.3e-35	147.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052733.t1	G7IU89	96.3	81	1	1	1	81	341	419	7.8e-34	152.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052732	MS.gene052733	0.810607	1.06E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGGCTGTTGAGGGATTCTT+TGG	0.214051	2.1:-7567656	MS.gene052733:CDS
TTGCTATCTCTGGTACAATA+AGG	0.288131	2.1:+7567685	None:intergenic
CCTGGTTAATTTGCTATCTC+TGG	0.395856	2.1:+7567675	None:intergenic
GGTTTCTTCTAGACAGTGAA+TGG	0.421240	2.1:+7567591	None:intergenic
AGGCTGTTGAGGGATTCTTT+GGG	0.428616	2.1:-7567655	MS.gene052733:CDS
TGCTATCTCTGGTACAATAA+GGG	0.429510	2.1:+7567686	None:intergenic
CAAATTAACCAGGCTGTTGA+GGG	0.455154	2.1:-7567665	MS.gene052733:CDS
GTGCATAATCCAAGGTACTA+AGG	0.462379	2.1:+7567569	None:intergenic
GCAAATTAACCAGGCTGTTG+AGG	0.480162	2.1:-7567666	MS.gene052733:CDS
AGAAGAAACCCTTAGTACCT+TGG	0.494706	2.1:-7567578	MS.gene052733:CDS
CTGTTGAGGGATTCTTTGGG+TGG	0.516949	2.1:-7567652	MS.gene052733:CDS
CAAAGAATCCCTCAACAGCC+TGG	0.546362	2.1:+7567657	None:intergenic
TGTGGCAATAATGCATGTCT+TGG	0.559202	2.1:+7567624	None:intergenic
TCTTCTAGACAGTGAATGGA+AGG	0.560957	2.1:+7567595	None:intergenic
TGCATAATCCAAGGTACTAA+GGG	0.583872	2.1:+7567570	None:intergenic
AGCACGGTGTGCATAATCCA+AGG	0.594273	2.1:+7567561	None:intergenic
CCAGAGATAGCAAATTAACC+AGG	0.606765	2.1:-7567675	MS.gene052733:CDS
GAAATTGACAGACTAAAGCA+AGG	0.618332	2.1:-7567377	None:intergenic
AAATATCAAGAACAAACCAG+AGG	0.713003	2.1:-7567536	MS.gene052733:intron
GTGAATGGAAGGTGATACTG+TGG	0.730036	2.1:+7567606	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTATTTTATAAAGCTGCAA+AGG	-	chr2.1:7567585-7567604	MS.gene052733:CDS	25.0%
!!	CAGCTTTATAAAATAGTGAA+AGG	+	chr2.1:7567582-7567601	None:intergenic	25.0%
!!!	TGTTCTTGATATTTTTAGCA+CGG	+	chr2.1:7567526-7567545	None:intergenic	25.0%
	AAATATCAAGAACAAACCAG+AGG	-	chr2.1:7567532-7567551	MS.gene052733:intron	30.0%
!!!	TGAAGTTGCTTGTTTTTTTC+AGG	-	chr2.1:7567621-7567640	MS.gene052733:CDS	30.0%
	AATAGTGAAAGGTTGACCAA+AGG	+	chr2.1:7567571-7567590	None:intergenic	35.0%
	AGCTGCAAAGGATATCTAAT+AGG	-	chr2.1:7567597-7567616	MS.gene052733:CDS	35.0%
	AGGTGACTCTAATATTCCTT+TGG	-	chr2.1:7567552-7567571	MS.gene052733:CDS	35.0%
	TGCTATCTCTGGTACAATAA+GGG	+	chr2.1:7567385-7567404	None:intergenic	35.0%
	TTGCTATCTCTGGTACAATA+AGG	+	chr2.1:7567386-7567405	None:intergenic	35.0%
!	TGCATAATCCAAGGTACTAA+GGG	+	chr2.1:7567501-7567520	None:intergenic	35.0%
	AGAAGAAACCCTTAGTACCT+TGG	-	chr2.1:7567490-7567509	MS.gene052733:intron	40.0%
	AGGAATATTAGAGTCACCTC+TGG	+	chr2.1:7567551-7567570	None:intergenic	40.0%
	CAAATTAACCAGGCTGTTGA+GGG	-	chr2.1:7567403-7567422	MS.gene052733:CDS	40.0%
	CCAGAGATAGCAAATTAACC+AGG	-	chr2.1:7567393-7567412	MS.gene052733:CDS	40.0%
	GGTTTCTTCTAGACAGTGAA+TGG	+	chr2.1:7567480-7567499	None:intergenic	40.0%
	TCTTCTAGACAGTGAATGGA+AGG	+	chr2.1:7567476-7567495	None:intergenic	40.0%
	TGTGGCAATAATGCATGTCT+TGG	+	chr2.1:7567447-7567466	None:intergenic	40.0%
!	GTGCATAATCCAAGGTACTA+AGG	+	chr2.1:7567502-7567521	None:intergenic	40.0%
!!	CCTGGTTAATTTGCTATCTC+TGG	+	chr2.1:7567396-7567415	None:intergenic	40.0%
	AGGCTGTTGAGGGATTCTTT+GGG	-	chr2.1:7567413-7567432	MS.gene052733:CDS	45.0%
	GCAAATTAACCAGGCTGTTG+AGG	-	chr2.1:7567402-7567421	MS.gene052733:CDS	45.0%
	GTGAATGGAAGGTGATACTG+TGG	+	chr2.1:7567465-7567484	None:intergenic	45.0%
	AGCACGGTGTGCATAATCCA+AGG	+	chr2.1:7567510-7567529	None:intergenic	50.0%
	CAAAGAATCCCTCAACAGCC+TGG	+	chr2.1:7567414-7567433	None:intergenic	50.0%
	CAGGCTGTTGAGGGATTCTT+TGG	-	chr2.1:7567412-7567431	MS.gene052733:CDS	50.0%
	CTGTTGAGGGATTCTTTGGG+TGG	-	chr2.1:7567416-7567435	MS.gene052733:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	7567378	7567712	7567378	ID=MS.gene052733
chr2.1	mRNA	7567378	7567712	7567378	ID=MS.gene052733.t1;Parent=MS.gene052733
chr2.1	exon	7567537	7567712	7567537	ID=MS.gene052733.t1.exon1;Parent=MS.gene052733.t1
chr2.1	CDS	7567537	7567712	7567537	ID=cds.MS.gene052733.t1;Parent=MS.gene052733.t1
chr2.1	exon	7567378	7567447	7567378	ID=MS.gene052733.t1.exon2;Parent=MS.gene052733.t1
chr2.1	CDS	7567378	7567447	7567378	ID=cds.MS.gene052733.t1;Parent=MS.gene052733.t1

Gene Sequence	>MS.gene052733 ATATCCCTTATTGTACCAGAGATAGCAAATTAACCAGGCTGTTGAGGGATTCTTTGGGTGGTAAAACCAAGACATGCATTATTGCCACAGTATCACCTTCCATTCACTGTCTAGAAGAAACCCTTAGTACCTTGGATTATGCACACCGTGCTAAAAATATCAAGAACAAACCAGAGGTGACTCTAATATTCCTTTGGTCAACCTTTCACTATTTTATAAAGCTGCAAAGGATATCTAATAGGTTGAAGTTGCTTGTTTTTTTCAGGTTAATCAGAAAATGATGAAATCTGCGATGATTAAAGATTTGTATTCTGAAATTGACAGACTAAAGCAAG
Protein sequence	>MS.gene052733.t1 ISLIVPEIANUPGCUGILWVVKPRHALLPQYHLPFTVUKKPLVPWIMHTVLKISRTNQRLIRKUUNLRULKICILKLTDUSK