Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054604.t1 | XP_003602973.1 | 79.6 | 383 | 76 | 1 | 43 | 425 | 9 | 389 | 4.90E-170 | 607.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054604.t1 | Q9S7T8 | 53.4 | 382 | 175 | 3 | 44 | 423 | 10 | 390 | 5.6e-114 | 412.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054604.t1 | G7J5Q8 | 79.6 | 383 | 76 | 1 | 43 | 425 | 9 | 389 | 3.5e-170 | 607.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene054604 | MS.gene065944 | PPI |
| MS.gene006610 | MS.gene054604 | PPI |
| MS.gene054604 | MS.gene031697 | PPI |
| MS.gene054604 | MS.gene045585 | PPI |
| MS.gene065945 | MS.gene054604 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054604.t1 | MTR_3g101030 | 73.286 | 423 | 92 | 5 | 22 | 425 | 9 | 429 | 0.0 | 604 |
| MS.gene054604.t1 | MTR_3g101050 | 76.941 | 425 | 69 | 7 | 1 | 424 | 1 | 397 | 0.0 | 602 |
| MS.gene054604.t1 | MTR_3g101010 | 79.634 | 383 | 76 | 1 | 43 | 425 | 9 | 389 | 0.0 | 597 |
| MS.gene054604.t1 | MTR_3g018740 | 71.802 | 383 | 103 | 3 | 42 | 423 | 8 | 386 | 0.0 | 534 |
| MS.gene054604.t1 | MTR_8g027395 | 67.959 | 387 | 118 | 2 | 39 | 425 | 17 | 397 | 0.0 | 519 |
| MS.gene054604.t1 | MTR_4g045707 | 66.410 | 390 | 127 | 3 | 36 | 425 | 3 | 388 | 8.00e-180 | 506 |
| MS.gene054604.t1 | MTR_8g027420 | 66.150 | 387 | 114 | 3 | 39 | 425 | 6 | 375 | 8.82e-179 | 503 |
| MS.gene054604.t1 | MTR_8g461160 | 65.535 | 383 | 130 | 1 | 43 | 423 | 10 | 392 | 1.60e-176 | 498 |
| MS.gene054604.t1 | MTR_3g101180 | 63.750 | 400 | 129 | 3 | 36 | 423 | 3 | 398 | 1.50e-175 | 496 |
| MS.gene054604.t1 | MTR_7g451890 | 63.636 | 385 | 138 | 1 | 43 | 425 | 18 | 402 | 4.96e-174 | 493 |
| MS.gene054604.t1 | MTR_7g047780 | 63.472 | 386 | 137 | 2 | 43 | 425 | 18 | 402 | 9.30e-172 | 487 |
| MS.gene054604.t1 | MTR_7g050750 | 63.377 | 385 | 139 | 1 | 43 | 425 | 12 | 396 | 1.27e-171 | 486 |
| MS.gene054604.t1 | MTR_4g088525 | 65.312 | 369 | 124 | 1 | 56 | 424 | 4 | 368 | 5.35e-170 | 481 |
| MS.gene054604.t1 | MTR_2g046030 | 64.767 | 386 | 125 | 4 | 42 | 423 | 8 | 386 | 6.16e-170 | 481 |
| MS.gene054604.t1 | MTR_3g101130 | 63.281 | 384 | 136 | 2 | 43 | 425 | 10 | 389 | 2.77e-169 | 480 |
| MS.gene054604.t1 | MTR_2g045980 | 65.026 | 386 | 124 | 4 | 42 | 423 | 8 | 386 | 4.61e-168 | 477 |
| MS.gene054604.t1 | MTR_7g050810 | 62.338 | 385 | 143 | 1 | 43 | 425 | 20 | 404 | 4.74e-168 | 477 |
| MS.gene054604.t1 | MTR_4g088535 | 65.625 | 384 | 110 | 4 | 37 | 420 | 5 | 366 | 3.11e-166 | 472 |
| MS.gene054604.t1 | MTR_3g048000 | 63.708 | 383 | 116 | 5 | 43 | 424 | 31 | 391 | 2.16e-165 | 470 |
| MS.gene054604.t1 | MTR_3g015620 | 61.917 | 386 | 117 | 6 | 42 | 423 | 19 | 378 | 1.15e-151 | 435 |
| MS.gene054604.t1 | MTR_3g102920 | 60.263 | 380 | 111 | 6 | 47 | 423 | 66 | 408 | 4.05e-144 | 417 |
| MS.gene054604.t1 | MTR_3g100995 | 74.016 | 254 | 63 | 2 | 172 | 425 | 14 | 264 | 7.83e-136 | 390 |
| MS.gene054604.t1 | MTR_3g101200 | 56.873 | 371 | 133 | 5 | 55 | 423 | 45 | 390 | 9.18e-133 | 387 |
| MS.gene054604.t1 | MTR_3g101190 | 55.068 | 365 | 97 | 3 | 63 | 425 | 13 | 312 | 6.22e-130 | 377 |
| MS.gene054604.t1 | MTR_3g018747 | 69.231 | 260 | 64 | 3 | 165 | 423 | 9 | 253 | 4.07e-121 | 352 |
| MS.gene054604.t1 | MTR_7g050830 | 55.519 | 308 | 106 | 2 | 45 | 332 | 22 | 318 | 2.07e-112 | 333 |
| MS.gene054604.t1 | MTR_3g100010 | 56.289 | 318 | 91 | 2 | 62 | 346 | 38 | 340 | 1.79e-111 | 331 |
| MS.gene054604.t1 | MTR_3g015760 | 66.045 | 268 | 87 | 2 | 157 | 423 | 15 | 279 | 2.82e-111 | 328 |
| MS.gene054604.t1 | MTR_3g111160 | 70.952 | 210 | 58 | 2 | 217 | 425 | 13 | 220 | 6.87e-95 | 284 |
| MS.gene054604.t1 | MTR_3g101110 | 61.317 | 243 | 64 | 4 | 165 | 403 | 68 | 284 | 2.67e-90 | 275 |
| MS.gene054604.t1 | MTR_3g100520 | 62.613 | 222 | 68 | 3 | 206 | 425 | 1 | 209 | 1.05e-88 | 268 |
| MS.gene054604.t1 | MTR_3g099970 | 66.327 | 196 | 49 | 2 | 165 | 360 | 20 | 198 | 7.22e-86 | 260 |
| MS.gene054604.t1 | MTR_3g114310 | 41.250 | 400 | 210 | 11 | 41 | 425 | 21 | 410 | 9.50e-86 | 268 |
| MS.gene054604.t1 | MTR_2g046410 | 66.010 | 203 | 63 | 2 | 225 | 423 | 147 | 347 | 1.11e-85 | 265 |
| MS.gene054604.t1 | MTR_3g101020 | 64.171 | 187 | 40 | 2 | 239 | 425 | 1 | 160 | 8.97e-76 | 233 |
| MS.gene054604.t1 | MTR_3g101150 | 70.253 | 158 | 43 | 2 | 271 | 424 | 1 | 158 | 2.86e-70 | 219 |
| MS.gene054604.t1 | MTR_3g102870 | 64.286 | 154 | 55 | 0 | 228 | 381 | 1 | 154 | 1.81e-63 | 202 |
| MS.gene054604.t1 | MTR_3g111150 | 58.989 | 178 | 47 | 3 | 42 | 219 | 2 | 153 | 7.07e-62 | 197 |
| MS.gene054604.t1 | MTR_3g100990 | 58.108 | 148 | 59 | 2 | 43 | 190 | 9 | 153 | 1.16e-44 | 162 |
| MS.gene054604.t1 | MTR_4g093550 | 69.512 | 82 | 23 | 1 | 43 | 124 | 10 | 89 | 5.70e-28 | 106 |
| MS.gene054604.t1 | MTR_3g101140 | 47.581 | 124 | 47 | 4 | 304 | 425 | 9 | 116 | 1.47e-20 | 87.0 |
| MS.gene054604.t1 | MTR_6g046890 | 63.158 | 76 | 25 | 1 | 57 | 132 | 15 | 87 | 1.58e-20 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054604.t1 | AT1G47710 | 53.403 | 382 | 175 | 3 | 44 | 423 | 10 | 390 | 1.39e-144 | 417 |
| MS.gene054604.t1 | AT1G47710 | 53.403 | 382 | 175 | 3 | 44 | 423 | 37 | 417 | 4.58e-144 | 417 |
| MS.gene054604.t1 | AT3G45220 | 48.477 | 394 | 177 | 6 | 43 | 422 | 9 | 390 | 1.62e-125 | 369 |
| MS.gene054604.t1 | AT2G25240 | 49.479 | 384 | 180 | 7 | 43 | 422 | 9 | 382 | 1.04e-121 | 359 |
| MS.gene054604.t1 | AT2G26390 | 48.593 | 391 | 177 | 7 | 43 | 422 | 9 | 386 | 5.18e-120 | 355 |
| MS.gene054604.t1 | AT2G14540 | 45.052 | 384 | 184 | 6 | 43 | 422 | 37 | 397 | 1.51e-96 | 295 |
| MS.gene054604.t1 | AT1G64030 | 43.005 | 386 | 191 | 6 | 43 | 422 | 9 | 371 | 5.91e-96 | 293 |
| MS.gene054604.t1 | AT1G62170 | 41.892 | 370 | 184 | 5 | 62 | 424 | 88 | 433 | 4.86e-95 | 292 |
| MS.gene054604.t1 | AT2G35580 | 43.733 | 375 | 180 | 9 | 58 | 422 | 18 | 371 | 2.07e-90 | 278 |
| MS.gene054604.t1 | AT1G62170 | 38.462 | 403 | 184 | 6 | 62 | 424 | 88 | 466 | 7.57e-88 | 275 |
| MS.gene054604.t1 | AT1G64010 | 45.872 | 218 | 94 | 4 | 206 | 422 | 1 | 195 | 5.77e-58 | 189 |
| MS.gene054604.t1 | AT1G64010 | 45.813 | 203 | 86 | 4 | 221 | 422 | 2 | 181 | 8.53e-52 | 172 |
| MS.gene054604.t1 | AT1G63280 | 54.783 | 115 | 48 | 2 | 165 | 277 | 8 | 120 | 3.00e-35 | 127 |
| MS.gene054604.t1 | AT1G62160 | 35.426 | 223 | 116 | 6 | 207 | 424 | 21 | 220 | 1.07e-31 | 120 |
| MS.gene054604.t1 | AT1G51330 | 49.550 | 111 | 53 | 2 | 164 | 271 | 30 | 140 | 4.44e-25 | 102 |
| MS.gene054604.t1 | AT1G64020 | 34.211 | 114 | 72 | 3 | 44 | 156 | 10 | 121 | 5.03e-15 | 71.6 |
Find 99 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGACTTGTGGAATATTT+TGG | 0.106119 | 3.4:-89008241 | None:intergenic |
| GCTAATGCACTATACTTTAA+AGG | 0.155210 | 3.4:+89007781 | MS.gene054604:CDS |
| CGAGAAAAGAAGTGAATTTA+TGG | 0.176628 | 3.4:+89007680 | MS.gene054604:CDS |
| TGAAACTTCTGATATGTTAA+AGG | 0.194021 | 3.4:+89008137 | MS.gene054604:CDS |
| TTATCTTCACCTTGCTTATA+AGG | 0.268250 | 3.4:-89007949 | None:intergenic |
| CTTGTCTTGATGAGATTCTT+TGG | 0.271973 | 3.4:-89007186 | None:intergenic |
| GACGGTGGCTGCAGCAGCTT+TGG | 0.307932 | 3.4:-89008286 | None:intergenic |
| AAGTGGAAGTAGGTGACTTT+AGG | 0.323594 | 3.4:+89008085 | MS.gene054604:CDS |
| GAGGAAGTCAAGGGCGTATT+CGG | 0.323880 | 3.4:-89007102 | None:intergenic |
| GCCAACTTTGCTTGAGAAGC+TGG | 0.324647 | 3.4:+89008020 | MS.gene054604:CDS |
| ATTCAAAAGGTGAAAGTCAT+AGG | 0.334305 | 3.4:-89007842 | None:intergenic |
| TTTCTTCCAAATGCAAAAGA+TGG | 0.344851 | 3.4:+89007994 | MS.gene054604:CDS |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.350146 | 3.4:+89008426 | MS.gene054604:CDS |
| TTCTTTGGAGAAAAGATGTT+TGG | 0.357142 | 3.4:-89007171 | None:intergenic |
| GAATGGAGGGTTGACTAAAA+TGG | 0.363840 | 3.4:+89008185 | MS.gene054604:CDS |
| GGTTGTTGGCGGGTTGGAAT+CGG | 0.375878 | 3.4:-89007124 | None:intergenic |
| ACCGGAACTATCGCCTTTGT+TGG | 0.400356 | 3.4:+89008402 | MS.gene054604:CDS |
| CAACAACTTCTCGACTTCCT+TGG | 0.417102 | 3.4:+89007284 | MS.gene054604:CDS |
| TGAAAGAAGGTTGAAGAGAA+AGG | 0.426729 | 3.4:-89007430 | None:intergenic |
| AACTATCGCCTTTGTTGGGC+AGG | 0.426929 | 3.4:+89008407 | MS.gene054604:CDS |
| ATTAAAGAACTTCTTCCTCT+AGG | 0.427888 | 3.4:+89007727 | MS.gene054604:CDS |
| CCCAACAAAGGCGATAGTTC+CGG | 0.429621 | 3.4:-89008403 | None:intergenic |
| GGAAACGTTGCTTTGGTTGT+TGG | 0.432490 | 3.4:-89007138 | None:intergenic |
| GACACAATTTGCTTGAAAGA+AGG | 0.436343 | 3.4:-89007443 | None:intergenic |
| GATAATGAGTGGGATCGATT+TGG | 0.439052 | 3.4:-89007060 | None:intergenic |
| TCTCTGATCATAAATAAGAA+TGG | 0.440676 | 3.4:-89008372 | None:intergenic |
| ACCGTCGCAACTTTACTTTA+CGG | 0.440844 | 3.4:+89008303 | MS.gene054604:CDS |
| TAACTGAGTTTCCATTCAAA+AGG | 0.441115 | 3.4:-89007855 | None:intergenic |
| AACGTTGCTTTGGTTGTTGG+CGG | 0.442357 | 3.4:-89007135 | None:intergenic |
| TTTGAATGGAAACTCAGTTA+AGG | 0.443112 | 3.4:+89007858 | MS.gene054604:CDS |
| TTAAAGAACTTCTTCCTCTA+GGG | 0.444956 | 3.4:+89007728 | MS.gene054604:CDS |
| CTCTGATCATAAATAAGAAT+GGG | 0.447141 | 3.4:-89008371 | None:intergenic |
| CAAGCTTCCTCTTGATAAAG+TGG | 0.447424 | 3.4:+89008068 | MS.gene054604:CDS |
| GAGAAAAGAAGTGAATTTAT+GGG | 0.455502 | 3.4:+89007681 | MS.gene054604:CDS |
| TTCATTGAAGTAAATGAAGA+AGG | 0.457204 | 3.4:+89008261 | MS.gene054604:CDS |
| GAAAGAAGGTTGAAGAGAAA+GGG | 0.460189 | 3.4:-89007429 | None:intergenic |
| TGCTTTGGTTGTTGGCGGGT+TGG | 0.465518 | 3.4:-89007130 | None:intergenic |
| CTTCTTCTCACTGGTCATGA+AGG | 0.470421 | 3.4:-89007884 | None:intergenic |
| CCGGAACTATCGCCTTTGTT+GGG | 0.480048 | 3.4:+89008403 | MS.gene054604:CDS |
| CGATGATGCTGAGCACAACT+TGG | 0.482600 | 3.4:-89007235 | None:intergenic |
| AAAGACGGTGCTCCCGCAGG+TGG | 0.483007 | 3.4:+89007365 | MS.gene054604:CDS |
| TGGTGAGGGAAACGTTGCTT+TGG | 0.486728 | 3.4:-89007145 | None:intergenic |
| TTCTTCCAAATGCAAAAGAT+GGG | 0.486946 | 3.4:+89007995 | MS.gene054604:CDS |
| GCCAGCTTCTCAAGCAAAGT+TGG | 0.489801 | 3.4:-89008021 | None:intergenic |
| GAAGAAGTAGAAAACAGCTT+TGG | 0.494264 | 3.4:-89006978 | None:intergenic |
| GTGTTGTAGTAACTCAGATT+CGG | 0.496759 | 3.4:-89008046 | None:intergenic |
| CTTCACCTTGCTTATAAGGA+AGG | 0.497179 | 3.4:-89007945 | None:intergenic |
| GAGAGACGAGGCCCACCTGC+GGG | 0.498754 | 3.4:-89007377 | None:intergenic |
| TCTGATATGTTAAAGGAGCT+TGG | 0.499230 | 3.4:+89008144 | MS.gene054604:CDS |
| TTACTTCAATGAAAGACTTG+TGG | 0.504558 | 3.4:-89008251 | None:intergenic |
| CATCATCGCTGATGGTTCTG+AGG | 0.506455 | 3.4:+89007250 | MS.gene054604:CDS |
| GGGAGCACCGTCTTTGAGAA+TGG | 0.518279 | 3.4:-89007357 | None:intergenic |
| CTCAAAGACGGTGCTCCCGC+AGG | 0.518597 | 3.4:+89007362 | MS.gene054604:CDS |
| AATAAACTGCTTCTTCTCAC+TGG | 0.523013 | 3.4:-89007893 | None:intergenic |
| TCAAATGAAATATTGAACCT+TGG | 0.524127 | 3.4:-89008111 | None:intergenic |
| AAAGATGTTTGGCGATGGTG+AGG | 0.524270 | 3.4:-89007160 | None:intergenic |
| GCTACAAAGTCTATGTCTGT+TGG | 0.529202 | 3.4:-89008342 | None:intergenic |
| TAGGTGACTTTAGGATTCCA+AGG | 0.533810 | 3.4:+89008094 | MS.gene054604:CDS |
| GAAGAAGTTTAGATGATCGA+TGG | 0.536342 | 3.4:-89007315 | None:intergenic |
| TTCTGTTGATTTCAAGAACA+AGG | 0.537281 | 3.4:+89007490 | MS.gene054604:CDS |
| ATGATCAGAGAAGATTTGAC+CGG | 0.538023 | 3.4:+89008384 | MS.gene054604:CDS |
| TGTTGGCGGGTTGGAATCGG+AGG | 0.542527 | 3.4:-89007121 | None:intergenic |
| GTTGGAATCGGAGGAAGTCA+AGG | 0.547443 | 3.4:-89007112 | None:intergenic |
| ACGTTGCTTTGGTTGTTGGC+GGG | 0.547721 | 3.4:-89007134 | None:intergenic |
| GTCAACCCTCCATTCGTGAA+AGG | 0.553572 | 3.4:-89008177 | None:intergenic |
| CACTATACTTTAAAGGAGAG+TGG | 0.554157 | 3.4:+89007788 | MS.gene054604:CDS |
| GTGCTCAGCATCATCGCTGA+TGG | 0.561012 | 3.4:+89007242 | MS.gene054604:CDS |
| AAGACGGTGCTCCCGCAGGT+GGG | 0.563207 | 3.4:+89007366 | MS.gene054604:CDS |
| CCTACTTCCACTTTATCAAG+AGG | 0.564451 | 3.4:-89008075 | None:intergenic |
| CCTCTTGATAAAGTGGAAGT+AGG | 0.566916 | 3.4:+89008075 | MS.gene054604:CDS |
| CTTGGCCTTCCTTATAAGCA+AGG | 0.569395 | 3.4:+89007940 | MS.gene054604:CDS |
| TTCTTCTCACTGGTCATGAA+GGG | 0.569786 | 3.4:-89007883 | None:intergenic |
| GAGTCGATGTTGATAATGAG+TGG | 0.573168 | 3.4:-89007071 | None:intergenic |
| TGGGCTGAAAGAGAGACAAA+TGG | 0.574537 | 3.4:+89007700 | MS.gene054604:CDS |
| GTCGAGAAGTTGTTGTTGAG+TGG | 0.576826 | 3.4:-89007276 | None:intergenic |
| TGCTAAGATCATCCAGCAAG+AGG | 0.578961 | 3.4:-89008438 | None:intergenic |
| AGAGAGACGAGGCCCACCTG+CGG | 0.584963 | 3.4:-89007378 | None:intergenic |
| ATCATCGCTGATGGTTCTGA+GGG | 0.591707 | 3.4:+89007251 | MS.gene054604:CDS |
| GTAAAGTAAAGTTGCGACGG+TGG | 0.595397 | 3.4:-89008301 | None:intergenic |
| AAGATGTTTGGCGATGGTGA+GGG | 0.596429 | 3.4:-89007159 | None:intergenic |
| ATTGAGCACCTGCCCAACAA+AGG | 0.599203 | 3.4:-89008415 | None:intergenic |
| GTAGAAAACAGCTTTGGCAA+TGG | 0.604998 | 3.4:-89006972 | None:intergenic |
| TTGGAATCGGAGGAAGTCAA+GGG | 0.608158 | 3.4:-89007111 | None:intergenic |
| CAAGGGCGTATTCGGATCGG+AGG | 0.609828 | 3.4:-89007094 | None:intergenic |
| TATGTCTGTTGGAAGACTCA+AGG | 0.610434 | 3.4:-89008331 | None:intergenic |
| AGAACTTCTTCCTCTAGGGT+CGG | 0.613652 | 3.4:+89007732 | MS.gene054604:CDS |
| AGTCAAGGGCGTATTCGGAT+CGG | 0.617677 | 3.4:-89007097 | None:intergenic |
| TGATCATAAATAAGAATGGG+TGG | 0.618025 | 3.4:-89008368 | None:intergenic |
| GTCTTCGCCATTCTCAAAGA+CGG | 0.621526 | 3.4:+89007350 | MS.gene054604:CDS |
| TCCGTAAAGTAAAGTTGCGA+CGG | 0.626090 | 3.4:-89008304 | None:intergenic |
| AGTCGATGTTGATAATGAGT+GGG | 0.634885 | 3.4:-89007070 | None:intergenic |
| TATGTTAAAGGAGCTTGGCG+TGG | 0.637410 | 3.4:+89008149 | MS.gene054604:CDS |
| CTTGCTTATAAGGAAGGCCA+AGG | 0.645897 | 3.4:-89007939 | None:intergenic |
| GGAGAAAAGATGTTTGGCGA+TGG | 0.656877 | 3.4:-89007165 | None:intergenic |
| AAGGGCGTATTCGGATCGGA+GGG | 0.661792 | 3.4:-89007093 | None:intergenic |
| GATCGATGGATTTAGAGCCA+AGG | 0.665025 | 3.4:-89007301 | None:intergenic |
| CCATCGACAAAAGAGAGACG+AGG | 0.674638 | 3.4:-89007389 | None:intergenic |
| AAGTTGTCGACCGACCCTAG+AGG | 0.702735 | 3.4:-89007742 | None:intergenic |
| AGCACAACTTGGATTGACAG+TGG | 0.707392 | 3.4:-89007224 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATTTTAATTTTTGATA+TGG | + | chr3.4:89007639-89007658 | MS.gene054604:intron | 10.0% |
| !!! | AATTTTTGATATGGTATTAA+AGG | + | chr3.4:89007648-89007667 | MS.gene054604:intron | 15.0% |
| !! | ACAAAATCAAAATCAAAATC+AGG | - | chr3.4:89007564-89007583 | None:intergenic | 20.0% |
| !! | AGAACTCAAAACTATATATT+TGG | + | chr3.4:89007613-89007632 | MS.gene054604:intron | 20.0% |
| !! | CAAAATCAAAATCAAAATCA+GGG | - | chr3.4:89007563-89007582 | None:intergenic | 20.0% |
| ! | ATATCTTGGGAAAAATGAAT+TGG | - | chr3.4:89007040-89007059 | None:intergenic | 25.0% |
| ! | CTCTGATCATAAATAAGAAT+GGG | - | chr3.4:89008374-89008393 | None:intergenic | 25.0% |
| ! | GAGAAAAGAAGTGAATTTAT+GGG | + | chr3.4:89007681-89007700 | MS.gene054604:CDS | 25.0% |
| ! | TCAAATGAAATATTGAACCT+TGG | - | chr3.4:89008114-89008133 | None:intergenic | 25.0% |
| ! | TCTCTGATCATAAATAAGAA+TGG | - | chr3.4:89008375-89008394 | None:intergenic | 25.0% |
| ! | TGAAACTTCTGATATGTTAA+AGG | + | chr3.4:89008137-89008156 | MS.gene054604:CDS | 25.0% |
| ! | TTCATTGAAGTAAATGAAGA+AGG | + | chr3.4:89008261-89008280 | MS.gene054604:CDS | 25.0% |
| !!! | GAATGATAATTTTGATGCTT+CGG | + | chr3.4:89007810-89007829 | MS.gene054604:CDS | 25.0% |
| !!! | GGAATATTTTGGAAACATAA+AGG | - | chr3.4:89008233-89008252 | None:intergenic | 25.0% |
| ATTAAAGAACTTCTTCCTCT+AGG | + | chr3.4:89007727-89007746 | MS.gene054604:CDS | 30.0% | |
| ATTCAAAAGGTGAAAGTCAT+AGG | - | chr3.4:89007845-89007864 | None:intergenic | 30.0% | |
| CGAGAAAAGAAGTGAATTTA+TGG | + | chr3.4:89007680-89007699 | MS.gene054604:CDS | 30.0% | |
| GCTAATGCACTATACTTTAA+AGG | + | chr3.4:89007781-89007800 | MS.gene054604:CDS | 30.0% | |
| TAACTGAGTTTCCATTCAAA+AGG | - | chr3.4:89007858-89007877 | None:intergenic | 30.0% | |
| TGATCATAAATAAGAATGGG+TGG | - | chr3.4:89008371-89008390 | None:intergenic | 30.0% | |
| TTAAAGAACTTCTTCCTCTA+GGG | + | chr3.4:89007728-89007747 | MS.gene054604:CDS | 30.0% | |
| TTACTTCAATGAAAGACTTG+TGG | - | chr3.4:89008254-89008273 | None:intergenic | 30.0% | |
| TTATCTTCACCTTGCTTATA+AGG | - | chr3.4:89007952-89007971 | None:intergenic | 30.0% | |
| TTCTTCCAAATGCAAAAGAT+GGG | + | chr3.4:89007995-89008014 | MS.gene054604:CDS | 30.0% | |
| TTTCTTCCAAATGCAAAAGA+TGG | + | chr3.4:89007994-89008013 | MS.gene054604:CDS | 30.0% | |
| TTTGAATGGAAACTCAGTTA+AGG | + | chr3.4:89007858-89007877 | MS.gene054604:CDS | 30.0% | |
| ! | GAAAGACTTGTGGAATATTT+TGG | - | chr3.4:89008244-89008263 | None:intergenic | 30.0% |
| ! | TATGACTTTCACCTTTTGAA+TGG | + | chr3.4:89007844-89007863 | MS.gene054604:CDS | 30.0% |
| ! | TGTATTTTGTAGCGAAAAGA+GGG | - | chr3.4:89007532-89007551 | None:intergenic | 30.0% |
| ! | TTCTTTGGAGAAAAGATGTT+TGG | - | chr3.4:89007174-89007193 | None:intergenic | 30.0% |
| ! | TTGTATTTTGTAGCGAAAAG+AGG | - | chr3.4:89007533-89007552 | None:intergenic | 30.0% |
| !! | TTCTGTTGATTTCAAGAACA+AGG | + | chr3.4:89007490-89007509 | MS.gene054604:CDS | 30.0% |
| !!! | TTCGATGATTTTAAAGTCCT+TGG | + | chr3.4:89007922-89007941 | MS.gene054604:CDS | 30.0% |
| AATAAACTGCTTCTTCTCAC+TGG | - | chr3.4:89007896-89007915 | None:intergenic | 35.0% | |
| ATGATCAGAGAAGATTTGAC+CGG | + | chr3.4:89008384-89008403 | MS.gene054604:CDS | 35.0% | |
| CTTGTCTTGATGAGATTCTT+TGG | - | chr3.4:89007189-89007208 | None:intergenic | 35.0% | |
| GAAAGAAGGTTGAAGAGAAA+GGG | - | chr3.4:89007432-89007451 | None:intergenic | 35.0% | |
| GAAGAAGTAGAAAACAGCTT+TGG | - | chr3.4:89006981-89007000 | None:intergenic | 35.0% | |
| GAAGAAGTTTAGATGATCGA+TGG | - | chr3.4:89007318-89007337 | None:intergenic | 35.0% | |
| GTGTTGTAGTAACTCAGATT+CGG | - | chr3.4:89008049-89008068 | None:intergenic | 35.0% | |
| TCTGATATGTTAAAGGAGCT+TGG | + | chr3.4:89008144-89008163 | MS.gene054604:CDS | 35.0% | |
| TGAAAGAAGGTTGAAGAGAA+AGG | - | chr3.4:89007433-89007452 | None:intergenic | 35.0% | |
| ! | CACTATACTTTAAAGGAGAG+TGG | + | chr3.4:89007788-89007807 | MS.gene054604:CDS | 35.0% |
| ! | GACACAATTTGCTTGAAAGA+AGG | - | chr3.4:89007446-89007465 | None:intergenic | 35.0% |
| ! | GTATTTTGTAGCGAAAAGAG+GGG | - | chr3.4:89007531-89007550 | None:intergenic | 35.0% |
| ! | TTTTACCTTTCACGAATGGA+GGG | + | chr3.4:89008172-89008191 | MS.gene054604:CDS | 35.0% |
| !! | AGTCGATGTTGATAATGAGT+GGG | - | chr3.4:89007073-89007092 | None:intergenic | 35.0% |
| !!! | ACATAAAGGTTTTGACTCAC+AGG | - | chr3.4:89008219-89008238 | None:intergenic | 35.0% |
| AAGTGGAAGTAGGTGACTTT+AGG | + | chr3.4:89008085-89008104 | MS.gene054604:CDS | 40.0% | |
| ACCGTCGCAACTTTACTTTA+CGG | + | chr3.4:89008303-89008322 | MS.gene054604:CDS | 40.0% | |
| CAAGCTTCCTCTTGATAAAG+TGG | + | chr3.4:89008068-89008087 | MS.gene054604:CDS | 40.0% | |
| CCTACTTCCACTTTATCAAG+AGG | - | chr3.4:89008078-89008097 | None:intergenic | 40.0% | |
| CCTCTTGATAAAGTGGAAGT+AGG | + | chr3.4:89008075-89008094 | MS.gene054604:CDS | 40.0% | |
| CTTCACCTTGCTTATAAGGA+AGG | - | chr3.4:89007948-89007967 | None:intergenic | 40.0% | |
| GAATGGAGGGTTGACTAAAA+TGG | + | chr3.4:89008185-89008204 | MS.gene054604:CDS | 40.0% | |
| GATAATGAGTGGGATCGATT+TGG | - | chr3.4:89007063-89007082 | None:intergenic | 40.0% | |
| GCTACAAAGTCTATGTCTGT+TGG | - | chr3.4:89008345-89008364 | None:intergenic | 40.0% | |
| GGGAAAAATGAATTGGACCA+AGG | - | chr3.4:89007033-89007052 | None:intergenic | 40.0% | |
| TATGTCTGTTGGAAGACTCA+AGG | - | chr3.4:89008334-89008353 | None:intergenic | 40.0% | |
| TCCGTAAAGTAAAGTTGCGA+CGG | - | chr3.4:89008307-89008326 | None:intergenic | 40.0% | |
| TTCTTCTCACTGGTCATGAA+GGG | - | chr3.4:89007886-89007905 | None:intergenic | 40.0% | |
| ! | GTAGAAAACAGCTTTGGCAA+TGG | - | chr3.4:89006975-89006994 | None:intergenic | 40.0% |
| ! | GTTTTACCTTTCACGAATGG+AGG | + | chr3.4:89008171-89008190 | MS.gene054604:CDS | 40.0% |
| !! | GAGTCGATGTTGATAATGAG+TGG | - | chr3.4:89007074-89007093 | None:intergenic | 40.0% |
| !! | GGGATCGATTTGGATATCTT+GGG | - | chr3.4:89007053-89007072 | None:intergenic | 40.0% |
| !! | GTGGCTTGTTTATTCATCCT+TGG | + | chr3.4:89007013-89007032 | MS.gene054604:intron | 40.0% |
| !! | GTGGTTTTACCTTTCACGAA+TGG | + | chr3.4:89008168-89008187 | MS.gene054604:CDS | 40.0% |
| !! | TAGGTGACTTTAGGATTCCA+AGG | + | chr3.4:89008094-89008113 | MS.gene054604:CDS | 40.0% |
| !! | TGGGATCGATTTGGATATCT+TGG | - | chr3.4:89007054-89007073 | None:intergenic | 40.0% |
| AAAGATGTTTGGCGATGGTG+AGG | - | chr3.4:89007163-89007182 | None:intergenic | 45.0% | |
| AAGATGTTTGGCGATGGTGA+GGG | - | chr3.4:89007162-89007181 | None:intergenic | 45.0% | |
| AGCACAACTTGGATTGACAG+TGG | - | chr3.4:89007227-89007246 | None:intergenic | 45.0% | |
| CAACAACTTCTCGACTTCCT+TGG | + | chr3.4:89007284-89007303 | MS.gene054604:CDS | 45.0% | |
| CTTCTTCATGCTCAACATCG+TGG | + | chr3.4:89006994-89007013 | MS.gene054604:intron | 45.0% | |
| CTTCTTCTCACTGGTCATGA+AGG | - | chr3.4:89007887-89007906 | None:intergenic | 45.0% | |
| CTTGCTTATAAGGAAGGCCA+AGG | - | chr3.4:89007942-89007961 | None:intergenic | 45.0% | |
| GGAGAAAAGATGTTTGGCGA+TGG | - | chr3.4:89007168-89007187 | None:intergenic | 45.0% | |
| GTAAAGTAAAGTTGCGACGG+TGG | - | chr3.4:89008304-89008323 | None:intergenic | 45.0% | |
| GTCTTCGCCATTCTCAAAGA+CGG | + | chr3.4:89007350-89007369 | MS.gene054604:CDS | 45.0% | |
| TATGTTAAAGGAGCTTGGCG+TGG | + | chr3.4:89008149-89008168 | MS.gene054604:CDS | 45.0% | |
| TGGGCTGAAAGAGAGACAAA+TGG | + | chr3.4:89007700-89007719 | MS.gene054604:CDS | 45.0% | |
| TTGGAATCGGAGGAAGTCAA+GGG | - | chr3.4:89007114-89007133 | None:intergenic | 45.0% | |
| ! | AACGTTGCTTTGGTTGTTGG+CGG | - | chr3.4:89007138-89007157 | None:intergenic | 45.0% |
| ! | CTCTTTTGTCGATGGTGTGT+GGG | + | chr3.4:89007397-89007416 | MS.gene054604:CDS | 45.0% |
| ! | CTTGGCCTTCCTTATAAGCA+AGG | + | chr3.4:89007940-89007959 | MS.gene054604:CDS | 45.0% |
| ! | GATCGATGGATTTAGAGCCA+AGG | - | chr3.4:89007304-89007323 | None:intergenic | 45.0% |
| ! | GGAAACGTTGCTTTGGTTGT+TGG | - | chr3.4:89007141-89007160 | None:intergenic | 45.0% |
| ! | GTCGAGAAGTTGTTGTTGAG+TGG | - | chr3.4:89007279-89007298 | None:intergenic | 45.0% |
| ! | TCTCTTTTGTCGATGGTGTG+TGG | + | chr3.4:89007396-89007415 | MS.gene054604:CDS | 45.0% |
| !! | AGAACTTCTTCCTCTAGGGT+CGG | + | chr3.4:89007732-89007751 | MS.gene054604:CDS | 45.0% |
| !! | ATCATCGCTGATGGTTCTGA+GGG | + | chr3.4:89007251-89007270 | MS.gene054604:CDS | 45.0% |
| AACTATCGCCTTTGTTGGGC+AGG | + | chr3.4:89008407-89008426 | MS.gene054604:CDS | 50.0% | |
| ACCGGAACTATCGCCTTTGT+TGG | + | chr3.4:89008402-89008421 | MS.gene054604:CDS | 50.0% | |
| ATTGAGCACCTGCCCAACAA+AGG | - | chr3.4:89008418-89008437 | None:intergenic | 50.0% | |
| CCATCGACAAAAGAGAGACG+AGG | - | chr3.4:89007392-89007411 | None:intergenic | 50.0% | |
| CCCAACAAAGGCGATAGTTC+CGG | - | chr3.4:89008406-89008425 | None:intergenic | 50.0% | |
| CCGGAACTATCGCCTTTGTT+GGG | + | chr3.4:89008403-89008422 | MS.gene054604:CDS | 50.0% | |
| CGATGATGCTGAGCACAACT+TGG | - | chr3.4:89007238-89007257 | None:intergenic | 50.0% | |
| GCCAACTTTGCTTGAGAAGC+TGG | + | chr3.4:89008020-89008039 | MS.gene054604:CDS | 50.0% | |
| GCCAGCTTCTCAAGCAAAGT+TGG | - | chr3.4:89008024-89008043 | None:intergenic | 50.0% | |
| GTCAACCCTCCATTCGTGAA+AGG | - | chr3.4:89008180-89008199 | None:intergenic | 50.0% | |
| GTTGGAATCGGAGGAAGTCA+AGG | - | chr3.4:89007115-89007134 | None:intergenic | 50.0% | |
| TGGTGAGGGAAACGTTGCTT+TGG | - | chr3.4:89007148-89007167 | None:intergenic | 50.0% | |
| ! | ACGTTGCTTTGGTTGTTGGC+GGG | - | chr3.4:89007137-89007156 | None:intergenic | 50.0% |
| ! | AGTCAAGGGCGTATTCGGAT+CGG | - | chr3.4:89007100-89007119 | None:intergenic | 50.0% |
| ! | CATCATCGCTGATGGTTCTG+AGG | + | chr3.4:89007250-89007269 | MS.gene054604:CDS | 50.0% |
| ! | CCTCGTCTCTCTTTTGTCGA+TGG | + | chr3.4:89007389-89007408 | MS.gene054604:CDS | 50.0% |
| ! | GAGGAAGTCAAGGGCGTATT+CGG | - | chr3.4:89007105-89007124 | None:intergenic | 50.0% |
| ! | GGCAGCCCATCTTTTGCATT+TGG | - | chr3.4:89008003-89008022 | None:intergenic | 50.0% |
| ! | TTTTGTCGATGGTGTGTGGG+TGG | + | chr3.4:89007400-89007419 | MS.gene054604:CDS | 50.0% |
| AAGTTGTCGACCGACCCTAG+AGG | - | chr3.4:89007745-89007764 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | + | chr3.4:89008426-89008445 | MS.gene054604:CDS | 55.0% | |
| GTGCTCAGCATCATCGCTGA+TGG | + | chr3.4:89007242-89007261 | MS.gene054604:CDS | 55.0% | |
| ! | AAGGGCGTATTCGGATCGGA+GGG | - | chr3.4:89007096-89007115 | None:intergenic | 55.0% |
| ! | TGCTTTGGTTGTTGGCGGGT+TGG | - | chr3.4:89007133-89007152 | None:intergenic | 55.0% |
| !! | GGGAGCACCGTCTTTGAGAA+TGG | - | chr3.4:89007360-89007379 | None:intergenic | 55.0% |
| !! | GGTTGTTGGCGGGTTGGAAT+CGG | - | chr3.4:89007127-89007146 | None:intergenic | 55.0% |
| ! | CAAGGGCGTATTCGGATCGG+AGG | - | chr3.4:89007097-89007116 | None:intergenic | 60.0% |
| !! | TGTTGGCGGGTTGGAATCGG+AGG | - | chr3.4:89007124-89007143 | None:intergenic | 60.0% |
| AAAGACGGTGCTCCCGCAGG+TGG | + | chr3.4:89007365-89007384 | MS.gene054604:CDS | 65.0% | |
| AAGACGGTGCTCCCGCAGGT+GGG | + | chr3.4:89007366-89007385 | MS.gene054604:CDS | 65.0% | |
| AGAGAGACGAGGCCCACCTG+CGG | - | chr3.4:89007381-89007400 | None:intergenic | 65.0% | |
| CTCAAAGACGGTGCTCCCGC+AGG | + | chr3.4:89007362-89007381 | MS.gene054604:CDS | 65.0% | |
| GACGGTGGCTGCAGCAGCTT+TGG | - | chr3.4:89008289-89008308 | None:intergenic | 65.0% | |
| GAGAGACGAGGCCCACCTGC+GGG | - | chr3.4:89007380-89007399 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 89006947 | 89008452 | 89006947 | ID=MS.gene054604 |
| chr3.4 | mRNA | 89006947 | 89008452 | 89006947 | ID=MS.gene054604.t1;Parent=MS.gene054604 |
| chr3.4 | exon | 89006947 | 89006985 | 89006947 | ID=MS.gene054604.t1.exon1;Parent=MS.gene054604.t1 |
| chr3.4 | CDS | 89006947 | 89006985 | 89006947 | ID=cds.MS.gene054604.t1;Parent=MS.gene054604.t1 |
| chr3.4 | exon | 89007056 | 89007511 | 89007056 | ID=MS.gene054604.t1.exon2;Parent=MS.gene054604.t1 |
| chr3.4 | CDS | 89007056 | 89007511 | 89007056 | ID=cds.MS.gene054604.t1;Parent=MS.gene054604.t1 |
| chr3.4 | exon | 89007670 | 89008452 | 89007670 | ID=MS.gene054604.t1.exon3;Parent=MS.gene054604.t1 |
| chr3.4 | CDS | 89007670 | 89008452 | 89007670 | ID=cds.MS.gene054604.t1;Parent=MS.gene054604.t1 |
| Gene Sequence |
| Protein sequence |
