Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054605.t1 | XP_003602973.1 | 75.8 | 385 | 88 | 3 | 55 | 436 | 5 | 387 | 6.20E-160 | 573.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054605.t1 | Q9S7T8 | 54.6 | 383 | 166 | 5 | 60 | 436 | 10 | 390 | 1.7e-113 | 411.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054605.t1 | G7J5Q8 | 75.8 | 385 | 88 | 3 | 55 | 436 | 5 | 387 | 4.5e-160 | 573.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene054605 | MS.gene045585 | PPI |
| MS.gene054605 | MS.gene065944 | PPI |
| MS.gene054605 | MS.gene065945 | PPI |
| MS.gene054605 | MS.gene031697 | PPI |
| MS.gene054605 | MS.gene006610 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054605.t1 | MTR_3g101030 | 69.087 | 427 | 109 | 6 | 31 | 437 | 5 | 428 | 0.0 | 573 |
| MS.gene054605.t1 | MTR_3g101010 | 75.648 | 386 | 89 | 3 | 55 | 437 | 5 | 388 | 0.0 | 569 |
| MS.gene054605.t1 | MTR_4g045707 | 69.072 | 388 | 115 | 3 | 52 | 437 | 3 | 387 | 0.0 | 534 |
| MS.gene054605.t1 | MTR_3g018740 | 70.909 | 385 | 106 | 3 | 55 | 436 | 5 | 386 | 0.0 | 529 |
| MS.gene054605.t1 | MTR_8g027395 | 68.229 | 384 | 115 | 3 | 55 | 436 | 17 | 395 | 0.0 | 520 |
| MS.gene054605.t1 | MTR_3g101050 | 70.025 | 397 | 94 | 8 | 48 | 437 | 19 | 397 | 0.0 | 518 |
| MS.gene054605.t1 | MTR_8g027420 | 67.969 | 384 | 105 | 4 | 55 | 436 | 6 | 373 | 5.11e-179 | 504 |
| MS.gene054605.t1 | MTR_3g101130 | 64.583 | 384 | 132 | 2 | 55 | 436 | 6 | 387 | 6.13e-174 | 492 |
| MS.gene054605.t1 | MTR_3g101180 | 65.657 | 396 | 125 | 4 | 52 | 436 | 3 | 398 | 7.41e-173 | 490 |
| MS.gene054605.t1 | MTR_4g088525 | 67.213 | 366 | 111 | 4 | 74 | 436 | 8 | 367 | 2.26e-172 | 488 |
| MS.gene054605.t1 | MTR_7g451890 | 61.209 | 397 | 149 | 2 | 45 | 436 | 4 | 400 | 2.18e-171 | 486 |
| MS.gene054605.t1 | MTR_7g050750 | 61.421 | 394 | 147 | 2 | 48 | 436 | 1 | 394 | 1.55e-169 | 481 |
| MS.gene054605.t1 | MTR_7g050810 | 61.578 | 393 | 140 | 4 | 52 | 436 | 13 | 402 | 4.77e-168 | 478 |
| MS.gene054605.t1 | MTR_7g047780 | 60.705 | 397 | 151 | 2 | 45 | 436 | 4 | 400 | 6.74e-168 | 478 |
| MS.gene054605.t1 | MTR_8g461160 | 63.239 | 389 | 138 | 3 | 53 | 436 | 4 | 392 | 1.26e-167 | 476 |
| MS.gene054605.t1 | MTR_3g048000 | 61.330 | 406 | 127 | 6 | 40 | 436 | 6 | 390 | 9.76e-167 | 474 |
| MS.gene054605.t1 | MTR_2g045980 | 62.113 | 388 | 135 | 5 | 55 | 436 | 5 | 386 | 6.09e-162 | 462 |
| MS.gene054605.t1 | MTR_4g088535 | 62.663 | 383 | 120 | 4 | 53 | 433 | 5 | 366 | 8.99e-162 | 461 |
| MS.gene054605.t1 | MTR_2g046030 | 61.598 | 388 | 137 | 5 | 55 | 436 | 5 | 386 | 6.16e-161 | 459 |
| MS.gene054605.t1 | MTR_3g015620 | 60.050 | 403 | 127 | 7 | 39 | 436 | 5 | 378 | 6.22e-147 | 423 |
| MS.gene054605.t1 | MTR_3g101200 | 55.696 | 395 | 144 | 7 | 46 | 436 | 23 | 390 | 1.91e-139 | 405 |
| MS.gene054605.t1 | MTR_3g100995 | 73.810 | 252 | 66 | 0 | 185 | 436 | 11 | 262 | 6.56e-138 | 396 |
| MS.gene054605.t1 | MTR_3g102920 | 53.012 | 415 | 147 | 7 | 29 | 436 | 35 | 408 | 4.02e-133 | 389 |
| MS.gene054605.t1 | MTR_3g101190 | 55.125 | 361 | 98 | 4 | 78 | 436 | 12 | 310 | 3.36e-122 | 358 |
| MS.gene054605.t1 | MTR_3g018747 | 67.181 | 259 | 68 | 2 | 181 | 436 | 9 | 253 | 1.69e-117 | 343 |
| MS.gene054605.t1 | MTR_3g100010 | 53.889 | 360 | 108 | 5 | 46 | 368 | 4 | 342 | 3.53e-117 | 346 |
| MS.gene054605.t1 | MTR_7g050830 | 52.584 | 329 | 125 | 2 | 41 | 349 | 2 | 319 | 3.01e-111 | 330 |
| MS.gene054605.t1 | MTR_3g015760 | 61.372 | 277 | 104 | 3 | 162 | 436 | 4 | 279 | 5.19e-104 | 310 |
| MS.gene054605.t1 | MTR_3g101110 | 60.251 | 239 | 62 | 3 | 181 | 412 | 68 | 280 | 1.30e-90 | 276 |
| MS.gene054605.t1 | MTR_3g100520 | 63.014 | 219 | 65 | 3 | 222 | 436 | 1 | 207 | 9.46e-90 | 271 |
| MS.gene054605.t1 | MTR_3g114310 | 42.065 | 397 | 203 | 12 | 57 | 435 | 20 | 407 | 2.72e-88 | 275 |
| MS.gene054605.t1 | MTR_3g111160 | 66.667 | 207 | 65 | 2 | 233 | 436 | 13 | 218 | 3.25e-88 | 268 |
| MS.gene054605.t1 | MTR_3g099970 | 68.718 | 195 | 44 | 2 | 181 | 374 | 20 | 198 | 4.78e-86 | 261 |
| MS.gene054605.t1 | MTR_2g046410 | 60.976 | 205 | 73 | 3 | 238 | 436 | 144 | 347 | 3.86e-78 | 246 |
| MS.gene054605.t1 | MTR_3g101020 | 63.187 | 182 | 43 | 1 | 255 | 436 | 1 | 158 | 2.45e-74 | 230 |
| MS.gene054605.t1 | MTR_3g101150 | 64.968 | 157 | 48 | 3 | 287 | 436 | 1 | 157 | 8.70e-61 | 195 |
| MS.gene054605.t1 | MTR_3g111150 | 57.627 | 177 | 49 | 3 | 59 | 235 | 3 | 153 | 3.70e-57 | 186 |
| MS.gene054605.t1 | MTR_3g102870 | 55.828 | 163 | 65 | 2 | 244 | 399 | 1 | 163 | 4.39e-55 | 181 |
| MS.gene054605.t1 | MTR_3g100990 | 68.702 | 131 | 39 | 1 | 55 | 185 | 5 | 133 | 5.73e-49 | 174 |
| MS.gene054605.t1 | MTR_4g093550 | 67.816 | 87 | 26 | 1 | 54 | 140 | 5 | 89 | 2.82e-29 | 110 |
| MS.gene054605.t1 | MTR_3g101140 | 47.934 | 121 | 48 | 3 | 319 | 437 | 8 | 115 | 6.08e-22 | 90.9 |
| MS.gene054605.t1 | MTR_6g046890 | 52.679 | 112 | 33 | 4 | 76 | 167 | 18 | 129 | 1.31e-20 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054605.t1 | AT1G47710 | 54.569 | 383 | 166 | 5 | 60 | 436 | 10 | 390 | 8.07e-147 | 424 |
| MS.gene054605.t1 | AT1G47710 | 54.569 | 383 | 166 | 5 | 60 | 436 | 37 | 417 | 5.77e-146 | 422 |
| MS.gene054605.t1 | AT3G45220 | 48.469 | 392 | 185 | 6 | 55 | 435 | 5 | 390 | 6.16e-127 | 373 |
| MS.gene054605.t1 | AT2G26390 | 47.716 | 394 | 181 | 7 | 55 | 435 | 5 | 386 | 2.55e-119 | 353 |
| MS.gene054605.t1 | AT2G25240 | 48.711 | 388 | 182 | 8 | 55 | 435 | 5 | 382 | 3.14e-119 | 353 |
| MS.gene054605.t1 | AT1G64030 | 43.896 | 385 | 190 | 6 | 59 | 437 | 9 | 373 | 2.23e-98 | 300 |
| MS.gene054605.t1 | AT2G14540 | 43.717 | 382 | 189 | 5 | 59 | 435 | 37 | 397 | 6.96e-95 | 291 |
| MS.gene054605.t1 | AT1G62170 | 41.530 | 366 | 184 | 5 | 78 | 435 | 88 | 431 | 4.21e-92 | 285 |
| MS.gene054605.t1 | AT2G35580 | 43.817 | 372 | 181 | 8 | 74 | 435 | 18 | 371 | 1.08e-91 | 282 |
| MS.gene054605.t1 | AT1G62170 | 38.095 | 399 | 184 | 6 | 78 | 435 | 88 | 464 | 3.85e-85 | 268 |
| MS.gene054605.t1 | AT1G64010 | 48.611 | 216 | 88 | 5 | 222 | 435 | 1 | 195 | 3.02e-62 | 200 |
| MS.gene054605.t1 | AT1G64010 | 47.761 | 201 | 82 | 5 | 237 | 435 | 2 | 181 | 7.64e-55 | 181 |
| MS.gene054605.t1 | AT1G63280 | 58.261 | 115 | 44 | 2 | 181 | 293 | 8 | 120 | 4.86e-38 | 134 |
| MS.gene054605.t1 | AT1G62160 | 39.106 | 179 | 87 | 4 | 259 | 435 | 60 | 218 | 2.70e-32 | 122 |
| MS.gene054605.t1 | AT1G51330 | 53.153 | 111 | 49 | 3 | 180 | 287 | 30 | 140 | 1.34e-25 | 103 |
| MS.gene054605.t1 | AT1G64020 | 32.743 | 113 | 73 | 3 | 60 | 171 | 10 | 120 | 1.45e-12 | 64.7 |
Find 100 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAATGCACTGTACTTTAA+AGG | 0.144123 | 3.4:+89013585 | MS.gene054605:CDS |
| TGATTAATAAAGTGAATTTA+TGG | 0.211117 | 3.4:+89013484 | MS.gene054605:CDS |
| TTTGGAAGCATCAAACTTAT+TGG | 0.242719 | 3.4:-89013616 | None:intergenic |
| CTTCTTCTTGCTGATCATAA+AGG | 0.256844 | 3.4:-89013688 | None:intergenic |
| GGAAACGTTGGTTTGGTTAA+TGG | 0.276967 | 3.4:-89012933 | None:intergenic |
| GTCAGACCTCCATTAGTAAA+AGG | 0.277817 | 3.4:-89013981 | None:intergenic |
| TTATCTTTGCCTTGCTTATA+CGG | 0.281478 | 3.4:-89013753 | None:intergenic |
| GAAAGACTTGTGAAATATAT+TGG | 0.284781 | 3.4:-89014039 | None:intergenic |
| GAAGAAGTTTAGATGATAGA+TGG | 0.319896 | 3.4:-89013110 | None:intergenic |
| CTCTCTGATGCCGCTCCCTT+TGG | 0.322428 | 3.4:+89013157 | MS.gene054605:CDS |
| AAAATCATAGTCTTTCGTTT+TGG | 0.334440 | 3.4:-89013634 | None:intergenic |
| CATCATAGCTGCTGGATCTC+AGG | 0.345380 | 3.4:+89013045 | MS.gene054605:CDS |
| TAATGGAGGTCTGACTAAAA+TGG | 0.358053 | 3.4:+89013989 | MS.gene054605:CDS |
| CAACAACTTCTCCACTTCCT+TGG | 0.372691 | 3.4:+89013079 | MS.gene054605:CDS |
| ACCGGAACTGTCGTCTTTGT+TGG | 0.377962 | 3.4:+89014197 | MS.gene054605:CDS |
| GATTAATAAAGTGAATTTAT+GGG | 0.381941 | 3.4:+89013485 | MS.gene054605:CDS |
| TTGTTCAACCCACACACTAT+TGG | 0.385574 | 3.4:-89013200 | None:intergenic |
| CAAACTTCCATTTGTTAAAG+TGG | 0.390173 | 3.4:+89013872 | MS.gene054605:CDS |
| GATTGAAGTGTGAAAGATAA+TGG | 0.396354 | 3.4:-89012813 | None:intergenic |
| TTCTTCTTGCTGATCATAAA+GGG | 0.399742 | 3.4:-89013687 | None:intergenic |
| CAAATGAAATATTGAACCTT+GGG | 0.407101 | 3.4:-89013914 | None:intergenic |
| GGTTTGGTTAATGGCAGATT+GGG | 0.427282 | 3.4:-89012924 | None:intergenic |
| AACTGTCGTCTTTGTTGGAC+AGG | 0.431215 | 3.4:+89014202 | MS.gene054605:CDS |
| TCTGATATGCTGAAGGAGTT+AGG | 0.433201 | 3.4:+89013948 | MS.gene054605:CDS |
| GGCAGCCCGTCTTTAGCATC+TGG | 0.437451 | 3.4:-89013804 | None:intergenic |
| TGAAGGAAGGTTGAAGGGGA+AGG | 0.438255 | 3.4:-89013225 | None:intergenic |
| GTGTTGTATCAACTCAGTTT+CGG | 0.441820 | 3.4:-89013850 | None:intergenic |
| TACGAGCCAGAGAAGATGTT+TGG | 0.455014 | 3.4:-89012774 | None:intergenic |
| CTCTGTTGCCAATAGTGTGT+GGG | 0.456798 | 3.4:+89013192 | MS.gene054605:CDS |
| GGACAGGTGCTCAATCCTCT+TGG | 0.458320 | 3.4:+89014218 | MS.gene054605:CDS |
| TCTGATGCCGCTCCCTTTGG+TGG | 0.459258 | 3.4:+89013160 | MS.gene054605:CDS |
| TTAAAGAAATTCTTCGTCAA+GGG | 0.460026 | 3.4:+89013532 | MS.gene054605:CDS |
| CTGGAGCAGTCGGCAGCTCC+AGG | 0.463577 | 3.4:+89014108 | MS.gene054605:CDS |
| TTCATTGAAGTAAACGAAGA+AGG | 0.464021 | 3.4:+89014059 | MS.gene054605:CDS |
| GAAGGAAGGTTGAAGGGGAA+GGG | 0.464022 | 3.4:-89013224 | None:intergenic |
| TCCAACAAAGACGACAGTTC+CGG | 0.465222 | 3.4:-89014198 | None:intergenic |
| AGTAGACACAGTTTCTTTGA+AGG | 0.465284 | 3.4:-89013242 | None:intergenic |
| CAAAGATGAGTCTGGTTAAG+TGG | 0.470681 | 3.4:-89013563 | None:intergenic |
| TGGTTTGGTTAATGGCAGAT+TGG | 0.472763 | 3.4:-89012925 | None:intergenic |
| CCTGCCTCCACTTTAACAAA+TGG | 0.473222 | 3.4:-89013879 | None:intergenic |
| TTTCTTTGAAGGAAGGTTGA+AGG | 0.474955 | 3.4:-89013231 | None:intergenic |
| GCTGCTGCGGTCACTATAGC+TGG | 0.475236 | 3.4:+89014089 | MS.gene054605:CDS |
| TGCATTTGCAAAGATGAGTC+TGG | 0.477055 | 3.4:-89013571 | None:intergenic |
| TCAAATGAAATATTGAACCT+TGG | 0.481541 | 3.4:-89013915 | None:intergenic |
| GTGCTCAGCATCATAGCTGC+TGG | 0.482388 | 3.4:+89013037 | MS.gene054605:CDS |
| CCATTTGTTAAAGTGGAGGC+AGG | 0.484875 | 3.4:+89013879 | MS.gene054605:CDS |
| CCATTGCCAAACATCTTCTC+TGG | 0.485386 | 3.4:+89012768 | MS.gene054605:CDS |
| CTATGATGCTGAGCACAACT+TGG | 0.485939 | 3.4:-89013030 | None:intergenic |
| GCGAGGACCACCAAAGGGAG+CGG | 0.502275 | 3.4:-89013167 | None:intergenic |
| TTCTTTGAAGGAAGGTTGAA+GGG | 0.503893 | 3.4:-89013230 | None:intergenic |
| ATTAAAGAAATTCTTCGTCA+AGG | 0.506528 | 3.4:+89013531 | MS.gene054605:CDS |
| TGGTGAGGGAAACGTTGGTT+TGG | 0.507864 | 3.4:-89012940 | None:intergenic |
| GGTTAATGGCAGATTGGGAT+CGG | 0.516446 | 3.4:-89012919 | None:intergenic |
| TATTCGAGTTGGAACAATCC+TGG | 0.518259 | 3.4:-89014126 | None:intergenic |
| TTCTTCCAGATGCTAAAGAC+GGG | 0.519130 | 3.4:+89013799 | MS.gene054605:CDS |
| TGGATTTAAAGCCAAGGAAG+TGG | 0.525756 | 3.4:-89013090 | None:intergenic |
| GAAATATATTGGAAATACAA+AGG | 0.530250 | 3.4:-89014028 | None:intergenic |
| AGAGAGGCGAGGACCACCAA+AGG | 0.532175 | 3.4:-89013173 | None:intergenic |
| ATGATCAGAGAAGATTTGAC+CGG | 0.538023 | 3.4:+89014179 | MS.gene054605:CDS |
| GACACAGTTTCTTTGAAGGA+AGG | 0.540665 | 3.4:-89013238 | None:intergenic |
| AGCGGCATCAGAGAGGATGA+TGG | 0.541979 | 3.4:-89013149 | None:intergenic |
| TTTCTTCCAGATGCTAAAGA+CGG | 0.548041 | 3.4:+89013798 | MS.gene054605:CDS |
| ATCATAGCTGCTGGATCTCA+GGG | 0.553300 | 3.4:+89013046 | MS.gene054605:CDS |
| TTCCGTTGATTTCAAGAACA+AGG | 0.553793 | 3.4:+89013285 | MS.gene054605:CDS |
| CACTGTACTTTAAAGGAGCA+TGG | 0.554893 | 3.4:+89013592 | MS.gene054605:CDS |
| GGCGATGGTGAGGGAAACGT+TGG | 0.556591 | 3.4:-89012945 | None:intergenic |
| AGAAGGCACTGAGGCTGCTG+CGG | 0.558311 | 3.4:+89014076 | MS.gene054605:CDS |
| TGGGCTGAGAAAGAGACAAA+TGG | 0.565306 | 3.4:+89013504 | MS.gene054605:CDS |
| AGAAATTCTTCGTCAAGGGT+CGG | 0.568942 | 3.4:+89013536 | MS.gene054605:CDS |
| GCTACAAAGTCTATTCGAGT+TGG | 0.573441 | 3.4:-89014137 | None:intergenic |
| TACCTTGTTCTTGAAATCAA+CGG | 0.574876 | 3.4:-89013287 | None:intergenic |
| TCTTTGAAGGAAGGTTGAAG+GGG | 0.577014 | 3.4:-89013229 | None:intergenic |
| GTCACTATAGCTGGAGCAGT+CGG | 0.586529 | 3.4:+89014098 | MS.gene054605:CDS |
| AGCTGACGATGTTCATGTGT+GGG | 0.587278 | 3.4:-89012884 | None:intergenic |
| TGAAACTTCTGATATGCTGA+AGG | 0.594035 | 3.4:+89013941 | MS.gene054605:CDS |
| AAGTGGAGGCAGGTGACTTG+AGG | 0.595381 | 3.4:+89013889 | MS.gene054605:CDS |
| CTTGGTCTTCCGTATAAGCA+AGG | 0.596297 | 3.4:+89013744 | MS.gene054605:CDS |
| GGCAACTTCTCAAGCAACGT+TGG | 0.597920 | 3.4:-89013825 | None:intergenic |
| ACTTCCATTTGTTAAAGTGG+AGG | 0.604072 | 3.4:+89013875 | MS.gene054605:CDS |
| TCTCTGTTGCCAATAGTGTG+TGG | 0.608134 | 3.4:+89013191 | MS.gene054605:CDS |
| TCTCAATGGCAGATCAGTCA+AGG | 0.611695 | 3.4:+89013662 | MS.gene054605:CDS |
| ATAGATGTTTGGCGATGGTG+AGG | 0.612058 | 3.4:-89012955 | None:intergenic |
| CCAGAGAAGATGTTTGGCAA+TGG | 0.616747 | 3.4:-89012768 | None:intergenic |
| CAGGTGACTTGAGGATCCCA+AGG | 0.619735 | 3.4:+89013898 | MS.gene054605:CDS |
| TGACTGATCTGCCATTGAGA+AGG | 0.622914 | 3.4:-89013659 | None:intergenic |
| GAGAGGCGAGGACCACCAAA+GGG | 0.625024 | 3.4:-89013172 | None:intergenic |
| TGTATCAACTCAGTTTCGGA+AGG | 0.630650 | 3.4:-89013846 | None:intergenic |
| GTGGAGAAGTTGTTGTTGAG+TGG | 0.637951 | 3.4:-89013071 | None:intergenic |
| AGCACAACTTGGATTGAGAG+TGG | 0.642348 | 3.4:-89013019 | None:intergenic |
| TAGATGTTTGGCGATGGTGA+GGG | 0.644133 | 3.4:-89012954 | None:intergenic |
| TGTGAATAGATGTTTGGCGA+TGG | 0.645801 | 3.4:-89012960 | None:intergenic |
| GATAGATGGATTTAAAGCCA+AGG | 0.649919 | 3.4:-89013096 | None:intergenic |
| TAATGGCAGATTGGGATCGG+AGG | 0.667018 | 3.4:-89012916 | None:intergenic |
| TATGCTGAAGGAGTTAGGCG+TGG | 0.677943 | 3.4:+89013953 | MS.gene054605:CDS |
| AAGCTGACGATGTTCATGTG+TGG | 0.681027 | 3.4:-89012885 | None:intergenic |
| CAAAGGGAGCGGCATCAGAG+AGG | 0.694948 | 3.4:-89013156 | None:intergenic |
| GCTCGCCAAGATCATCCAAG+AGG | 0.725110 | 3.4:-89014233 | None:intergenic |
| ACACACTATTGGCAACAGAG+AGG | 0.733111 | 3.4:-89013189 | None:intergenic |
| CTATTGGCAACAGAGAGGCG+AGG | 0.753182 | 3.4:-89013184 | None:intergenic |
| AGTAAACGAAGAAGGCACTG+AGG | 0.767944 | 3.4:+89014067 | MS.gene054605:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATAAAGTGAATTTAT+GGG | + | chr3.4:89013485-89013504 | MS.gene054605:CDS | 15.0% |
| !! | TGATTAATAAAGTGAATTTA+TGG | + | chr3.4:89013484-89013503 | MS.gene054605:CDS | 15.0% |
| !!! | GAATTTAAATTTTTAACAGT+TGG | - | chr3.4:89013374-89013393 | None:intergenic | 15.0% |
| !! | CTCTGATCATAAATAAAAAA+GGG | - | chr3.4:89014169-89014188 | None:intergenic | 20.0% |
| !! | GAAATATATTGGAAATACAA+AGG | - | chr3.4:89014031-89014050 | None:intergenic | 20.0% |
| !! | TCAAAAACGAATAGTATTAT+TGG | + | chr3.4:89012848-89012867 | MS.gene054605:CDS | 20.0% |
| !! | TCTCTGATCATAAATAAAAA+AGG | - | chr3.4:89014170-89014189 | None:intergenic | 20.0% |
| !!! | ATAAATTGACATGGTTTTAA+AGG | + | chr3.4:89013452-89013471 | MS.gene054605:intron | 20.0% |
| !!! | ATAACACTATATTTTTGTCT+TGG | - | chr3.4:89012997-89013016 | None:intergenic | 20.0% |
| !!! | TTAAATTTTTAACAGTTGGA+TGG | - | chr3.4:89013370-89013389 | None:intergenic | 20.0% |
| ! | AAGAAAATTGTTGTGAAAAG+AGG | - | chr3.4:89013330-89013349 | None:intergenic | 25.0% |
| ! | AGAAAATTGTTGTGAAAAGA+GGG | - | chr3.4:89013329-89013348 | None:intergenic | 25.0% |
| ! | ATTAAAGAAATTCTTCGTCA+AGG | + | chr3.4:89013531-89013550 | MS.gene054605:CDS | 25.0% |
| ! | CAAATGAAATATTGAACCTT+GGG | - | chr3.4:89013917-89013936 | None:intergenic | 25.0% |
| ! | GAAAGACTTGTGAAATATAT+TGG | - | chr3.4:89014042-89014061 | None:intergenic | 25.0% |
| ! | TCAAATGAAATATTGAACCT+TGG | - | chr3.4:89013918-89013937 | None:intergenic | 25.0% |
| ! | TGACTGTTAATAAATTGACA+TGG | + | chr3.4:89013443-89013462 | MS.gene054605:intron | 25.0% |
| ! | TGATCATAAATAAAAAAGGG+TGG | - | chr3.4:89014166-89014185 | None:intergenic | 25.0% |
| ! | TTAAAGAAATTCTTCGTCAA+GGG | + | chr3.4:89013532-89013551 | MS.gene054605:CDS | 25.0% |
| !! | AAAATCATAGTCTTTCGTTT+TGG | - | chr3.4:89013637-89013656 | None:intergenic | 25.0% |
| !! | TTCTTTTGTGAATAGATGTT+TGG | - | chr3.4:89012969-89012988 | None:intergenic | 25.0% |
| !!! | TTTGATGGTTTTAAAGTTCT+TGG | + | chr3.4:89013726-89013745 | MS.gene054605:CDS | 25.0% |
| GAAGAAGTTTAGATGATAGA+TGG | - | chr3.4:89013113-89013132 | None:intergenic | 30.0% | |
| GATTGAAGTGTGAAAGATAA+TGG | - | chr3.4:89012816-89012835 | None:intergenic | 30.0% | |
| TACCTTGTTCTTGAAATCAA+CGG | - | chr3.4:89013290-89013309 | None:intergenic | 30.0% | |
| TTATCTTTGCCTTGCTTATA+CGG | - | chr3.4:89013756-89013775 | None:intergenic | 30.0% | |
| TTCATTGAAGTAAACGAAGA+AGG | + | chr3.4:89014059-89014078 | MS.gene054605:CDS | 30.0% | |
| TTCTTCTTGCTGATCATAAA+GGG | - | chr3.4:89013690-89013709 | None:intergenic | 30.0% | |
| ! | CAAACTTCCATTTGTTAAAG+TGG | + | chr3.4:89013872-89013891 | MS.gene054605:CDS | 30.0% |
| ! | CAGTTTATTAGAGCTTTTGA+TGG | + | chr3.4:89013711-89013730 | MS.gene054605:CDS | 30.0% |
| ! | GTTTTACCTTTTACTAATGG+AGG | + | chr3.4:89013975-89013994 | MS.gene054605:CDS | 30.0% |
| ! | TATGATTTTCACCTTCTCAA+TGG | + | chr3.4:89013648-89013667 | MS.gene054605:CDS | 30.0% |
| !! | GTGGTTTTACCTTTTACTAA+TGG | + | chr3.4:89013972-89013991 | MS.gene054605:CDS | 30.0% |
| !! | TTTGGAAGCATCAAACTTAT+TGG | - | chr3.4:89013619-89013638 | None:intergenic | 30.0% |
| AGTAGACACAGTTTCTTTGA+AGG | - | chr3.4:89013245-89013264 | None:intergenic | 35.0% | |
| ATGATCAGAGAAGATTTGAC+CGG | + | chr3.4:89014179-89014198 | MS.gene054605:CDS | 35.0% | |
| CTTCTTCTTGCTGATCATAA+AGG | - | chr3.4:89013691-89013710 | None:intergenic | 35.0% | |
| GCAAATGCACTGTACTTTAA+AGG | + | chr3.4:89013585-89013604 | MS.gene054605:CDS | 35.0% | |
| GTGTTGTATCAACTCAGTTT+CGG | - | chr3.4:89013853-89013872 | None:intergenic | 35.0% | |
| TAATGGAGGTCTGACTAAAA+TGG | + | chr3.4:89013989-89014008 | MS.gene054605:CDS | 35.0% | |
| TGAAACTTCTGATATGCTGA+AGG | + | chr3.4:89013941-89013960 | MS.gene054605:CDS | 35.0% | |
| TTTCTTCCAGATGCTAAAGA+CGG | + | chr3.4:89013798-89013817 | MS.gene054605:CDS | 35.0% | |
| ! | ACTTCCATTTGTTAAAGTGG+AGG | + | chr3.4:89013875-89013894 | MS.gene054605:CDS | 35.0% |
| ! | GATAGATGGATTTAAAGCCA+AGG | - | chr3.4:89013099-89013118 | None:intergenic | 35.0% |
| ! | TTCTTTGAAGGAAGGTTGAA+GGG | - | chr3.4:89013233-89013252 | None:intergenic | 35.0% |
| ! | TTTCTTTGAAGGAAGGTTGA+AGG | - | chr3.4:89013234-89013253 | None:intergenic | 35.0% |
| !! | TTCCGTTGATTTCAAGAACA+AGG | + | chr3.4:89013285-89013304 | MS.gene054605:CDS | 35.0% |
| AGAAATTCTTCGTCAAGGGT+CGG | + | chr3.4:89013536-89013555 | MS.gene054605:CDS | 40.0% | |
| CAAAGATGAGTCTGGTTAAG+TGG | - | chr3.4:89013566-89013585 | None:intergenic | 40.0% | |
| GCTACAAAGTCTATTCGAGT+TGG | - | chr3.4:89014140-89014159 | None:intergenic | 40.0% | |
| GTCAGACCTCCATTAGTAAA+AGG | - | chr3.4:89013984-89014003 | None:intergenic | 40.0% | |
| TCTGATATGCTGAAGGAGTT+AGG | + | chr3.4:89013948-89013967 | MS.gene054605:CDS | 40.0% | |
| TGCATTTGCAAAGATGAGTC+TGG | - | chr3.4:89013574-89013593 | None:intergenic | 40.0% | |
| TGTATCAACTCAGTTTCGGA+AGG | - | chr3.4:89013849-89013868 | None:intergenic | 40.0% | |
| TGTGAATAGATGTTTGGCGA+TGG | - | chr3.4:89012963-89012982 | None:intergenic | 40.0% | |
| TTCTTCCAGATGCTAAAGAC+GGG | + | chr3.4:89013799-89013818 | MS.gene054605:CDS | 40.0% | |
| TTGTTCAACCCACACACTAT+TGG | - | chr3.4:89013203-89013222 | None:intergenic | 40.0% | |
| ! | CACTGTACTTTAAAGGAGCA+TGG | + | chr3.4:89013592-89013611 | MS.gene054605:CDS | 40.0% |
| ! | GACACAGTTTCTTTGAAGGA+AGG | - | chr3.4:89013241-89013260 | None:intergenic | 40.0% |
| ! | GGTTTGGTTAATGGCAGATT+GGG | - | chr3.4:89012927-89012946 | None:intergenic | 40.0% |
| ! | TCTTTGAAGGAAGGTTGAAG+GGG | - | chr3.4:89013232-89013251 | None:intergenic | 40.0% |
| ! | TGGATTTAAAGCCAAGGAAG+TGG | - | chr3.4:89013093-89013112 | None:intergenic | 40.0% |
| !! | GGAAACGTTGGTTTGGTTAA+TGG | - | chr3.4:89012936-89012955 | None:intergenic | 40.0% |
| !! | TATTCGAGTTGGAACAATCC+TGG | - | chr3.4:89014129-89014148 | None:intergenic | 40.0% |
| !! | TGGTTTGGTTAATGGCAGAT+TGG | - | chr3.4:89012928-89012947 | None:intergenic | 40.0% |
| AACTGTCGTCTTTGTTGGAC+AGG | + | chr3.4:89014202-89014221 | MS.gene054605:CDS | 45.0% | |
| AAGCTGACGATGTTCATGTG+TGG | - | chr3.4:89012888-89012907 | None:intergenic | 45.0% | |
| ACACACTATTGGCAACAGAG+AGG | - | chr3.4:89013192-89013211 | None:intergenic | 45.0% | |
| AGCACAACTTGGATTGAGAG+TGG | - | chr3.4:89013022-89013041 | None:intergenic | 45.0% | |
| AGCTGACGATGTTCATGTGT+GGG | - | chr3.4:89012887-89012906 | None:intergenic | 45.0% | |
| ATAGATGTTTGGCGATGGTG+AGG | - | chr3.4:89012958-89012977 | None:intergenic | 45.0% | |
| ATCATAGCTGCTGGATCTCA+GGG | + | chr3.4:89013046-89013065 | MS.gene054605:CDS | 45.0% | |
| CAACAACTTCTCCACTTCCT+TGG | + | chr3.4:89013079-89013098 | MS.gene054605:CDS | 45.0% | |
| CCAGAGAAGATGTTTGGCAA+TGG | - | chr3.4:89012771-89012790 | None:intergenic | 45.0% | |
| CCATTGCCAAACATCTTCTC+TGG | + | chr3.4:89012768-89012787 | MS.gene054605:CDS | 45.0% | |
| CCTGCCTCCACTTTAACAAA+TGG | - | chr3.4:89013882-89013901 | None:intergenic | 45.0% | |
| CTATGATGCTGAGCACAACT+TGG | - | chr3.4:89013033-89013052 | None:intergenic | 45.0% | |
| CTCTGTTGCCAATAGTGTGT+GGG | + | chr3.4:89013192-89013211 | MS.gene054605:CDS | 45.0% | |
| GGTTAATGGCAGATTGGGAT+CGG | - | chr3.4:89012922-89012941 | None:intergenic | 45.0% | |
| TACGAGCCAGAGAAGATGTT+TGG | - | chr3.4:89012777-89012796 | None:intergenic | 45.0% | |
| TAGATGTTTGGCGATGGTGA+GGG | - | chr3.4:89012957-89012976 | None:intergenic | 45.0% | |
| TCCAACAAAGACGACAGTTC+CGG | - | chr3.4:89014201-89014220 | None:intergenic | 45.0% | |
| TCTCAATGGCAGATCAGTCA+AGG | + | chr3.4:89013662-89013681 | MS.gene054605:CDS | 45.0% | |
| TCTCTGTTGCCAATAGTGTG+TGG | + | chr3.4:89013191-89013210 | MS.gene054605:CDS | 45.0% | |
| TGACTGATCTGCCATTGAGA+AGG | - | chr3.4:89013662-89013681 | None:intergenic | 45.0% | |
| TGGGCTGAGAAAGAGACAAA+TGG | + | chr3.4:89013504-89013523 | MS.gene054605:CDS | 45.0% | |
| ! | CCATTTGTTAAAGTGGAGGC+AGG | + | chr3.4:89013879-89013898 | MS.gene054605:CDS | 45.0% |
| ! | CTTGGTCTTCCGTATAAGCA+AGG | + | chr3.4:89013744-89013763 | MS.gene054605:CDS | 45.0% |
| ! | GTGGAGAAGTTGTTGTTGAG+TGG | - | chr3.4:89013074-89013093 | None:intergenic | 45.0% |
| !! | AGTAAACGAAGAAGGCACTG+AGG | + | chr3.4:89014067-89014086 | MS.gene054605:CDS | 45.0% |
| ACCGGAACTGTCGTCTTTGT+TGG | + | chr3.4:89014197-89014216 | MS.gene054605:CDS | 50.0% | |
| CATCATAGCTGCTGGATCTC+AGG | + | chr3.4:89013045-89013064 | MS.gene054605:CDS | 50.0% | |
| GAAGGAAGGTTGAAGGGGAA+GGG | - | chr3.4:89013227-89013246 | None:intergenic | 50.0% | |
| GGCAACTTCTCAAGCAACGT+TGG | - | chr3.4:89013828-89013847 | None:intergenic | 50.0% | |
| GTCACTATAGCTGGAGCAGT+CGG | + | chr3.4:89014098-89014117 | MS.gene054605:CDS | 50.0% | |
| TAATGGCAGATTGGGATCGG+AGG | - | chr3.4:89012919-89012938 | None:intergenic | 50.0% | |
| TATGCTGAAGGAGTTAGGCG+TGG | + | chr3.4:89013953-89013972 | MS.gene054605:CDS | 50.0% | |
| TGAAGGAAGGTTGAAGGGGA+AGG | - | chr3.4:89013228-89013247 | None:intergenic | 50.0% | |
| ! | TGGTGAGGGAAACGTTGGTT+TGG | - | chr3.4:89012943-89012962 | None:intergenic | 50.0% |
| AAGTGGAGGCAGGTGACTTG+AGG | + | chr3.4:89013889-89013908 | MS.gene054605:CDS | 55.0% | |
| AGCGGCATCAGAGAGGATGA+TGG | - | chr3.4:89013152-89013171 | None:intergenic | 55.0% | |
| CAGGTGACTTGAGGATCCCA+AGG | + | chr3.4:89013898-89013917 | MS.gene054605:CDS | 55.0% | |
| CTATTGGCAACAGAGAGGCG+AGG | - | chr3.4:89013187-89013206 | None:intergenic | 55.0% | |
| GGACAGGTGCTCAATCCTCT+TGG | + | chr3.4:89014218-89014237 | MS.gene054605:CDS | 55.0% | |
| GTGCTCAGCATCATAGCTGC+TGG | + | chr3.4:89013037-89013056 | MS.gene054605:CDS | 55.0% | |
| AGAGAGGCGAGGACCACCAA+AGG | - | chr3.4:89013176-89013195 | None:intergenic | 60.0% | |
| CAAAGGGAGCGGCATCAGAG+AGG | - | chr3.4:89013159-89013178 | None:intergenic | 60.0% | |
| CTCTCTGATGCCGCTCCCTT+TGG | + | chr3.4:89013157-89013176 | MS.gene054605:CDS | 60.0% | |
| GAGAGGCGAGGACCACCAAA+GGG | - | chr3.4:89013175-89013194 | None:intergenic | 60.0% | |
| GCTGCTGCGGTCACTATAGC+TGG | + | chr3.4:89014089-89014108 | MS.gene054605:CDS | 60.0% | |
| GGCAGCCCGTCTTTAGCATC+TGG | - | chr3.4:89013807-89013826 | None:intergenic | 60.0% | |
| GGCGATGGTGAGGGAAACGT+TGG | - | chr3.4:89012948-89012967 | None:intergenic | 60.0% | |
| TCTGATGCCGCTCCCTTTGG+TGG | + | chr3.4:89013160-89013179 | MS.gene054605:CDS | 60.0% | |
| !! | AGAAGGCACTGAGGCTGCTG+CGG | + | chr3.4:89014076-89014095 | MS.gene054605:CDS | 60.0% |
| GCGAGGACCACCAAAGGGAG+CGG | - | chr3.4:89013170-89013189 | None:intergenic | 65.0% | |
| CTGGAGCAGTCGGCAGCTCC+AGG | + | chr3.4:89014108-89014127 | MS.gene054605:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 89012764 | 89014244 | 89012764 | ID=MS.gene054605 |
| chr3.4 | mRNA | 89012764 | 89014244 | 89012764 | ID=MS.gene054605.t1;Parent=MS.gene054605 |
| chr3.4 | exon | 89012764 | 89013306 | 89012764 | ID=MS.gene054605.t1.exon1;Parent=MS.gene054605.t1 |
| chr3.4 | CDS | 89012764 | 89013306 | 89012764 | ID=cds.MS.gene054605.t1;Parent=MS.gene054605.t1 |
| chr3.4 | exon | 89013474 | 89014244 | 89013474 | ID=MS.gene054605.t1.exon2;Parent=MS.gene054605.t1 |
| chr3.4 | CDS | 89013474 | 89014244 | 89013474 | ID=cds.MS.gene054605.t1;Parent=MS.gene054605.t1 |
| Gene Sequence |
| Protein sequence |
