Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054668.t1 | XP_003614060.2 | 95 | 605 | 29 | 1 | 1 | 605 | 1 | 604 | 0.00E+00 | 1145.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054668.t1 | Q9FZD1 | 63.5 | 562 | 194 | 3 | 51 | 601 | 62 | 623 | 4.1e-211 | 735.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054668.t1 | G7JWU3 | 95.0 | 605 | 29 | 1 | 1 | 605 | 1 | 604 | 0.0e+00 | 1145.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035805 | MS.gene054668 | PPI |
| MS.gene054668 | MS.gene35108 | PPI |
| MS.gene054668 | MS.gene28721 | PPI |
| MS.gene054668 | MS.gene028350 | PPI |
| MS.gene35110 | MS.gene054668 | PPI |
| MS.gene064542 | MS.gene054668 | PPI |
| MS.gene36441 | MS.gene054668 | PPI |
| MS.gene054668 | MS.gene73119 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054668.t1 | MTR_5g044270 | 96.198 | 605 | 22 | 1 | 1 | 605 | 1 | 604 | 0.0 | 1204 |
| MS.gene054668.t1 | MTR_6g024415 | 95.413 | 109 | 5 | 0 | 288 | 396 | 3 | 111 | 2.17e-65 | 212 |
| MS.gene054668.t1 | MTR_1g009040 | 59.615 | 156 | 39 | 4 | 5 | 159 | 1 | 133 | 1.63e-49 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054668.t1 | AT1G26460 | 60.674 | 623 | 223 | 7 | 1 | 601 | 1 | 623 | 0.0 | 778 |
Find 137 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCAATTGTTTCTACTTT+TGG | 0.135748 | 5.3:-32077635 | MS.gene054668:CDS |
| ATTTGTTTAATCATTATATT+AGG | 0.156430 | 5.3:-32081030 | MS.gene054668:CDS |
| CTATTATGGCCTGGAGTTTA+TGG | 0.184716 | 5.3:-32078422 | MS.gene054668:CDS |
| GATTGATTTGATTGTGAAAT+TGG | 0.186157 | 5.3:+32081381 | None:intergenic |
| TGAAGGATGGGTTAGGAGTT+TGG | 0.215682 | 5.3:-32081086 | MS.gene054668:CDS |
| AGGAGCAGCATGGGCTGTTT+TGG | 0.238246 | 5.3:-32078284 | MS.gene054668:CDS |
| AAATTAACATCAGGTTTATT+AGG | 0.243041 | 5.3:+32081049 | None:intergenic |
| AATGGATGTAAAGAAGTAAA+TGG | 0.243768 | 5.3:+32078100 | None:intergenic |
| CCCCACTCTACAACGAAAAT+TGG | 0.270895 | 5.3:-32081258 | MS.gene054668:CDS |
| TTTGAGCATGCAACAGATAA+TGG | 0.279170 | 5.3:+32078082 | None:intergenic |
| TCCTAGCAGCTCAGAATTAT+AGG | 0.283390 | 5.3:+32078303 | None:intergenic |
| AAATTAAAACTCGCTGTATT+CGG | 0.301446 | 5.3:+32080932 | None:intergenic |
| GTTTGGGCACATAGTAAGTT+TGG | 0.305274 | 5.3:-32074160 | MS.gene054668:CDS |
| TCGGGTTCTTGATTGAGATA+TGG | 0.306238 | 5.3:+32081349 | None:intergenic |
| CATAAACTCCAGGCCATAAT+AGG | 0.307963 | 5.3:+32078423 | None:intergenic |
| AATAGCAAATTAGCCTATTA+TGG | 0.308171 | 5.3:-32078436 | MS.gene054668:CDS |
| CATGATTATGAGCAAGCTTA+TGG | 0.308715 | 5.3:-32078157 | MS.gene054668:CDS |
| CGTGAAGTTTCAAAGGTGTT+TGG | 0.315096 | 5.3:-32074177 | MS.gene054668:CDS |
| GCGAGATAAGATTCTGGATT+TGG | 0.315401 | 5.3:+32078235 | None:intergenic |
| AAACAATTGATTCAAAAGTT+TGG | 0.321687 | 5.3:+32077645 | None:intergenic |
| ACCTCATCTTTAACCCCATT+TGG | 0.328878 | 5.3:-32081217 | MS.gene054668:CDS |
| ATCTTTGCGAGATAAGATTC+TGG | 0.329322 | 5.3:+32078229 | None:intergenic |
| TATGAAAATGCACAATTTCC+AGG | 0.338364 | 5.3:+32078483 | None:intergenic |
| GGGGATCCTGAAGCTGGATT+TGG | 0.341174 | 5.3:+32081277 | None:intergenic |
| GATATATGCCCTTGCATCAT+TGG | 0.349384 | 5.3:-32078209 | MS.gene054668:CDS |
| ATTTGACATCCCGGTTAATC+AGG | 0.351971 | 5.3:+32074091 | None:intergenic |
| TGGTGTGGGGTCTACAAGTT+CGG | 0.354920 | 5.3:+32081330 | None:intergenic |
| GCGCTAAACTGTATCATTGT+AGG | 0.358136 | 5.3:-32077701 | MS.gene054668:CDS |
| CCATTGTGCGAATGAATATT+AGG | 0.358922 | 5.3:+32077605 | None:intergenic |
| GAATCATTTGACTTGGTCAT+TGG | 0.359618 | 5.3:-32078799 | MS.gene054668:CDS |
| ATTAGGGCTAATCTTATGAT+TGG | 0.367116 | 5.3:-32081013 | MS.gene054668:CDS |
| TCGATGCCCACCTGATTAAC+CGG | 0.368565 | 5.3:-32074101 | MS.gene054668:CDS |
| TATGGGAGTGCTTGATGATA+TGG | 0.373361 | 5.3:-32074064 | MS.gene054668:intron |
| AGGCAATACGACTTTGCATT+AGG | 0.374621 | 5.3:+32074129 | None:intergenic |
| TTTGGGCACATAGTAAGTTT+GGG | 0.376407 | 5.3:-32074159 | MS.gene054668:CDS |
| CGGGTTCTTGATTGAGATAT+GGG | 0.376922 | 5.3:+32081350 | None:intergenic |
| TGCATTTGGGAAGGCCAATA+AGG | 0.377932 | 5.3:-32077573 | MS.gene054668:intron |
| AGTAAGATCCATCACCTTAT+TGG | 0.380285 | 5.3:+32077559 | None:intergenic |
| ACAAAGCCCTTTGCCCCTCC+TGG | 0.381258 | 5.3:-32078501 | MS.gene054668:CDS |
| TTGTAGGATCTGCGAACATA+TGG | 0.381386 | 5.3:-32077685 | MS.gene054668:CDS |
| GATCCTGAAGCTGGATTTGG+TGG | 0.389737 | 5.3:+32081280 | None:intergenic |
| TTTGTTTAATCATTATATTA+GGG | 0.392061 | 5.3:-32081029 | MS.gene054668:CDS |
| GCTTGATTTGTTAGCTCAGA+TGG | 0.397920 | 5.3:-32080972 | MS.gene054668:CDS |
| CTCCCACCAAATCCAGCTTC+AGG | 0.401623 | 5.3:-32081283 | MS.gene054668:CDS |
| GTGAAGTTTCAAAGGTGTTT+GGG | 0.404120 | 5.3:-32074176 | MS.gene054668:CDS |
| GTTATCAGGATGCTGCAATC+TGG | 0.406078 | 5.3:-32078844 | MS.gene054668:intron |
| TCTTAATTACAGTACTGAAA+TGG | 0.406262 | 5.3:-32073799 | MS.gene054668:CDS |
| AAGTCAAATGATTCATCATC+AGG | 0.420113 | 5.3:+32078808 | None:intergenic |
| AATGGGCTGATGTATGCATT+TGG | 0.421515 | 5.3:-32077587 | MS.gene054668:CDS |
| ATTTATTTGCAGATAGCTGC+TGG | 0.422592 | 5.3:-32073969 | MS.gene054668:intron |
| GGCCATCTACAAAAGGCATT+TGG | 0.424516 | 5.3:-32078187 | MS.gene054668:CDS |
| ATCTGTTGCATGCTCAAAGA+AGG | 0.425706 | 5.3:-32078077 | MS.gene054668:CDS |
| ATTGATTTGATTGTGAAATT+GGG | 0.427484 | 5.3:+32081382 | None:intergenic |
| ATGGATGTAAAGAAGTAAAT+GGG | 0.436831 | 5.3:+32078101 | None:intergenic |
| CACTGAAAGCGATGAAAGAC+TGG | 0.438329 | 5.3:-32073886 | MS.gene054668:CDS |
| GGTGTGGGGTCTACAAGTTC+GGG | 0.441233 | 5.3:+32081331 | None:intergenic |
| CTAATATTCATTCGCACAAT+GGG | 0.441539 | 5.3:-32077604 | MS.gene054668:CDS |
| GGGAGTGATGATTCGGTTGG+TGG | 0.441954 | 5.3:+32081301 | None:intergenic |
| GATTCGGTTGGTGGTGGTGG+TGG | 0.445001 | 5.3:+32081310 | None:intergenic |
| GATGATTCGGTTGGTGGTGG+TGG | 0.445036 | 5.3:+32081307 | None:intergenic |
| TTGGGTAGCTTCACAACGTT+GGG | 0.445343 | 5.3:-32081128 | MS.gene054668:CDS |
| CGATCTTACAATTACCGACT+TGG | 0.449042 | 5.3:-32073852 | MS.gene054668:CDS |
| GGTTCTCAAGGCTATGCATC+AGG | 0.450261 | 5.3:-32080909 | MS.gene054668:CDS |
| ATATATGCCCTTGCATCATT+GGG | 0.454900 | 5.3:-32078208 | MS.gene054668:CDS |
| AAATGTTTGAAGGATGGGTT+AGG | 0.459710 | 5.3:-32081093 | MS.gene054668:CDS |
| GTGTTGCTTTCTGTAGATGA+AGG | 0.461555 | 5.3:-32078361 | MS.gene054668:CDS |
| GATTAAACAGCATGTTTCGT+CGG | 0.463811 | 5.3:+32073820 | None:intergenic |
| ATGGCTCGATGGATTGTAAA+GGG | 0.468300 | 5.3:-32078403 | MS.gene054668:CDS |
| TGATTAAACAAATTAACATC+AGG | 0.470842 | 5.3:+32081040 | None:intergenic |
| CAATTTCCAGGAGGGGCAAA+GGG | 0.475476 | 5.3:+32078495 | None:intergenic |
| AGTGATGATTCGGTTGGTGG+TGG | 0.476811 | 5.3:+32081304 | None:intergenic |
| CCGGGATGTCAAATCTGCTA+TGG | 0.480468 | 5.3:-32074082 | MS.gene054668:CDS |
| AGCGCAGCAACAGACTTGTA+TGG | 0.481918 | 5.3:+32077719 | None:intergenic |
| TAGAGTGGGGATCCTGAAGC+TGG | 0.482043 | 5.3:+32081271 | None:intergenic |
| ATTAAACAGCATGTTTCGTC+GGG | 0.488375 | 5.3:+32073821 | None:intergenic |
| CAAAAGCGCGTTGGAATCGT+AGG | 0.489632 | 5.3:+32081165 | None:intergenic |
| ATGGGCTGATGTATGCATTT+GGG | 0.492973 | 5.3:-32077586 | MS.gene054668:CDS |
| TCAGGTGGGCATCGATAAGC+AGG | 0.497019 | 5.3:+32074109 | None:intergenic |
| TGAAGGAAATGTTTGAAGGA+TGG | 0.497761 | 5.3:-32081099 | MS.gene054668:CDS |
| CCTAATATTCATTCGCACAA+TGG | 0.508619 | 5.3:-32077605 | MS.gene054668:CDS |
| ATTTGGTGGGAGTGATGATT+CGG | 0.510748 | 5.3:+32081294 | None:intergenic |
| ACAAGGAAGGCTTGATACAT+TGG | 0.516589 | 5.3:-32078659 | MS.gene054668:CDS |
| GGTGGGAGTGATGATTCGGT+TGG | 0.518921 | 5.3:+32081298 | None:intergenic |
| ATCATTGGGCCATCTACAAA+AGG | 0.524550 | 5.3:-32078194 | MS.gene054668:CDS |
| GCGCGTGTGAAATGACCAAA+TGG | 0.529121 | 5.3:+32081202 | None:intergenic |
| CAAATTAGCCTATTATGGCC+TGG | 0.529386 | 5.3:-32078431 | MS.gene054668:CDS |
| AAGTTTGGTAAGCTCGATCA+AGG | 0.529861 | 5.3:+32077660 | None:intergenic |
| ACAATTTCCAGGAGGGGCAA+AGG | 0.530807 | 5.3:+32078494 | None:intergenic |
| GTGTCAGCTCTGATGACTGC+AGG | 0.542612 | 5.3:-32078331 | MS.gene054668:CDS |
| CGGGATGTCAAATCTGCTAT+GGG | 0.544855 | 5.3:-32074081 | MS.gene054668:CDS |
| TGGCAATAATTGAAAAGTGC+AGG | 0.545019 | 5.3:-32078636 | MS.gene054668:CDS |
| GCAGACACGTGAAGTTTCAA+AGG | 0.545561 | 5.3:-32074184 | MS.gene054668:intron |
| CTGCTGAGAAACTTCTTGAA+CGG | 0.549508 | 5.3:-32080871 | MS.gene054668:intron |
| CTGGATTTGAACCAATGAAA+GGG | 0.552798 | 5.3:-32073950 | MS.gene054668:CDS |
| CGATGCCCACCTGATTAACC+GGG | 0.553923 | 5.3:-32074100 | MS.gene054668:CDS |
| GATGTGAAGGAAATGTTTGA+AGG | 0.554620 | 5.3:-32081103 | MS.gene054668:CDS |
| TCTGTTGCATGCTCAAAGAA+GGG | 0.556751 | 5.3:-32078076 | MS.gene054668:CDS |
| CTGAGCTGCTAGGAGCAGCA+TGG | 0.557087 | 5.3:-32078294 | MS.gene054668:CDS |
| TATGGCTCGATGGATTGTAA+AGG | 0.558390 | 5.3:-32078404 | MS.gene054668:CDS |
| ACCAAATGGGGTTAAAGATG+AGG | 0.558402 | 5.3:+32081216 | None:intergenic |
| GCCTGGAGTTTATGGCTCGA+TGG | 0.561213 | 5.3:-32078414 | MS.gene054668:CDS |
| ATCCTGAAGCTGGATTTGGT+GGG | 0.565034 | 5.3:+32081281 | None:intergenic |
| TGATGATGAATCATTTGACT+TGG | 0.565954 | 5.3:-32078806 | MS.gene054668:CDS |
| CGCGTGTGAAATGACCAAAT+GGG | 0.566353 | 5.3:+32081203 | None:intergenic |
| TGAGCTGCTAGGAGCAGCAT+GGG | 0.569572 | 5.3:-32078293 | MS.gene054668:CDS |
| TCCATCGAGCCATAAACTCC+AGG | 0.571109 | 5.3:+32078413 | None:intergenic |
| TGTAGGATCTGCGAACATAT+GGG | 0.572027 | 5.3:-32077684 | MS.gene054668:CDS |
| GAAGGAAATGTTTGAAGGAT+GGG | 0.576137 | 5.3:-32081098 | MS.gene054668:CDS |
| GCTGGATTTGAACCAATGAA+AGG | 0.576534 | 5.3:-32073951 | MS.gene054668:CDS |
| CTCAAGGCTATGCATCAGGC+TGG | 0.577861 | 5.3:-32080905 | MS.gene054668:CDS |
| TGACATCCCGGTTAATCAGG+TGG | 0.581779 | 5.3:+32074094 | None:intergenic |
| GTAGATGGCCCAATGATGCA+AGG | 0.582438 | 5.3:+32078200 | None:intergenic |
| ACCTATAATTCTGAGCTGCT+AGG | 0.584992 | 5.3:-32078304 | MS.gene054668:CDS |
| ATTGGGTAGCTTCACAACGT+TGG | 0.592801 | 5.3:-32081129 | MS.gene054668:CDS |
| AAAATGCACAATTTCCAGGA+GGG | 0.604881 | 5.3:+32078487 | None:intergenic |
| AGGAAGGCTTGATACATTGG+TGG | 0.606481 | 5.3:-32078656 | MS.gene054668:CDS |
| GACATCCCGGTTAATCAGGT+GGG | 0.607363 | 5.3:+32074095 | None:intergenic |
| GAAAACATTCAAAAGCGCGT+TGG | 0.609360 | 5.3:+32081156 | None:intergenic |
| ACAACGTTGGGATGATGTGA+AGG | 0.611093 | 5.3:-32081116 | MS.gene054668:CDS |
| AAATGCACAATTTCCAGGAG+GGG | 0.611198 | 5.3:+32078488 | None:intergenic |
| ACATGGTAACACGTTGTCAG+TGG | 0.611871 | 5.3:-32078716 | MS.gene054668:CDS |
| CCATAGCAGATTTGACATCC+CGG | 0.613360 | 5.3:+32074082 | None:intergenic |
| TGCAGTCATCAGAGCTGACA+CGG | 0.624877 | 5.3:+32078333 | None:intergenic |
| TAGATGGCCCAATGATGCAA+GGG | 0.631417 | 5.3:+32078201 | None:intergenic |
| GGAGTTGTGTCAAACAAGGA+AGG | 0.640031 | 5.3:-32078672 | MS.gene054668:CDS |
| AGCTGAAGTTGCAATTCGAG+CGG | 0.642238 | 5.3:-32078461 | MS.gene054668:CDS |
| GTCGGGCTTCAGAACCAAGT+CGG | 0.648802 | 5.3:+32073838 | None:intergenic |
| GCGTGTGAAATGACCAAATG+GGG | 0.653600 | 5.3:+32081204 | None:intergenic |
| ACTTGTATGGACGCTCAGCA+TGG | 0.661731 | 5.3:+32077732 | None:intergenic |
| TCATCTACAGAAAGCAACAC+AGG | 0.661970 | 5.3:+32078364 | None:intergenic |
| GGCAATAATTGAAAAGTGCA+GGG | 0.665279 | 5.3:-32078635 | MS.gene054668:intron |
| ATAGATTCAACTCTAAAACA+TGG | 0.670661 | 5.3:-32078733 | MS.gene054668:CDS |
| GTGAGGAGTTGTGTCAAACA+AGG | 0.685959 | 5.3:-32078676 | MS.gene054668:CDS |
| ATTTATTTATGAAATGTGTG+AGG | 0.693600 | 5.3:-32078693 | MS.gene054668:CDS |
| GCTGATGTATGCATTTGGGA+AGG | 0.709918 | 5.3:-32077582 | MS.gene054668:CDS |
| GAAAATGCACAATTTCCAGG+AGG | 0.712171 | 5.3:+32078486 | None:intergenic |
| TCTACAGAAAGCAACACAGG+AGG | 0.717369 | 5.3:+32078367 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 32073794 | 32081455 | 32073794 | ID=MS.gene054668 |
| chr5.3 | mRNA | 32073794 | 32081455 | 32073794 | ID=MS.gene054668.t1;Parent=MS.gene054668 |
| chr5.3 | exon | 32080872 | 32081455 | 32080872 | ID=MS.gene054668.t1.exon1;Parent=MS.gene054668.t1 |
| chr5.3 | CDS | 32080872 | 32081455 | 32080872 | ID=cds.MS.gene054668.t1;Parent=MS.gene054668.t1 |
| chr5.3 | exon | 32078636 | 32078858 | 32078636 | ID=MS.gene054668.t1.exon2;Parent=MS.gene054668.t1 |
| chr5.3 | CDS | 32078636 | 32078858 | 32078636 | ID=cds.MS.gene054668.t1;Parent=MS.gene054668.t1 |
| chr5.3 | exon | 32078057 | 32078536 | 32078057 | ID=MS.gene054668.t1.exon3;Parent=MS.gene054668.t1 |
| chr5.3 | CDS | 32078057 | 32078536 | 32078057 | ID=cds.MS.gene054668.t1;Parent=MS.gene054668.t1 |
| chr5.3 | exon | 32077574 | 32077780 | 32077574 | ID=MS.gene054668.t1.exon4;Parent=MS.gene054668.t1 |
| chr5.3 | CDS | 32077574 | 32077780 | 32077574 | ID=cds.MS.gene054668.t1;Parent=MS.gene054668.t1 |
| chr5.3 | exon | 32074065 | 32074202 | 32074065 | ID=MS.gene054668.t1.exon5;Parent=MS.gene054668.t1 |
| chr5.3 | CDS | 32074065 | 32074202 | 32074065 | ID=cds.MS.gene054668.t1;Parent=MS.gene054668.t1 |
| chr5.3 | exon | 32073794 | 32073979 | 32073794 | ID=MS.gene054668.t1.exon6;Parent=MS.gene054668.t1 |
| chr5.3 | CDS | 32073794 | 32073979 | 32073794 | ID=cds.MS.gene054668.t1;Parent=MS.gene054668.t1 |
| Gene Sequence |
| Protein sequence |
