AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054731

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054731.t1 MTR_5g044930 91.679 673 46 3 1 672 1 664 0.0 1222
MS.gene054731.t1 MTR_5g045620 91.679 673 42 4 1 672 1 660 0.0 1220
MS.gene054731.t1 MTR_4g081540 34.226 336 209 6 343 670 454 785 1.67e-59 214
MS.gene054731.t1 MTR_1g066900 25.369 678 422 18 66 670 35 701 2.00e-45 173
MS.gene054731.t1 MTR_4g099200 21.966 651 411 17 85 671 69 686 6.53e-35 141
MS.gene054731.t1 MTR_5g459290 92.982 57 4 0 180 236 26 82 1.22e-31 118
MS.gene054731.t1 MTR_6g022260 23.219 379 267 9 212 583 147 508 1.30e-17 87.0
MS.gene054731.t1 MTR_6g079920 21.340 567 371 15 123 644 17 553 8.41e-17 84.7
MS.gene054731.t1 MTR_2g049740 23.618 398 247 9 205 565 176 553 8.44e-17 85.1
MS.gene054731.t1 MTR_1g048080 24.928 345 221 8 254 567 469 806 1.28e-16 84.7
MS.gene054731.t1 MTR_4g068360 24.719 356 216 9 247 564 236 577 2.03e-16 83.6
MS.gene054731.t1 MTR_4g068360 21.654 508 342 18 112 595 61 536 3.28e-12 70.1
MS.gene054731.t1 MTR_1g068960 22.692 520 321 15 102 597 59 521 3.13e-16 83.2
MS.gene054731.t1 MTR_1g068960 26.857 175 127 1 395 569 394 567 4.09e-12 70.1
MS.gene054731.t1 MTR_3g037490 21.273 597 370 17 1 576 24 541 3.39e-16 82.8
MS.gene054731.t1 MTR_3g037490 22.308 390 251 8 234 623 129 466 6.26e-11 65.9
MS.gene054731.t1 MTR_6g078160 24.561 285 195 4 302 581 234 503 1.48e-15 80.5
MS.gene054731.t1 MTR_6g079830 22.144 569 364 16 123 644 17 553 1.49e-15 80.9
MS.gene054731.t1 MTR_2g037740 24.803 254 185 4 342 595 130 377 1.01e-14 78.6
MS.gene054731.t1 MTR_5g090170 21.318 516 326 16 151 622 199 678 1.19e-14 78.2
MS.gene054731.t1 MTR_5g090170 21.318 516 326 16 151 622 199 678 1.31e-14 78.2
MS.gene054731.t1 MTR_1g050495 24.242 297 207 6 302 595 236 517 1.37e-14 77.4
MS.gene054731.t1 MTR_1g031720 24.444 360 254 8 224 580 309 653 1.42e-14 78.2
MS.gene054731.t1 MTR_8g083130 23.604 394 269 9 225 614 125 490 6.53e-14 75.5
MS.gene054731.t1 MTR_8g083130 21.340 403 279 11 241 637 69 439 8.38e-11 65.5
MS.gene054731.t1 MTR_5g095130 23.585 212 157 4 384 594 593 800 8.02e-14 75.5
MS.gene054731.t1 MTR_6g079590 21.164 378 274 8 223 595 233 591 1.46e-13 74.3
MS.gene054731.t1 MTR_2g035630 22.509 271 199 4 302 572 236 495 1.71e-13 73.9
MS.gene054731.t1 MTR_7g091410 22.302 417 279 12 225 627 288 673 1.94e-13 74.3
MS.gene054731.t1 MTR_8g054410 23.132 281 206 3 302 582 215 485 2.49e-13 73.6
MS.gene054731.t1 MTR_6g079380 24.151 265 180 3 302 566 246 489 2.75e-13 73.2
MS.gene054731.t1 MTR_3g061000 23.790 248 171 4 396 638 157 391 3.37e-13 72.8
MS.gene054731.t1 MTR_3g061000 24.268 239 169 5 342 577 281 510 3.37e-13 72.8
MS.gene054731.t1 MTR_7g091410 22.302 417 279 12 225 627 149 534 3.38e-13 73.6
MS.gene054731.t1 MTR_7g091410 22.302 417 279 12 225 627 84 469 5.43e-13 72.8
MS.gene054731.t1 MTR_7g091410 22.302 417 279 12 225 627 43 428 7.34e-13 72.0
MS.gene054731.t1 MTR_8g105170 26.214 206 150 2 379 583 242 446 9.82e-13 72.4
MS.gene054731.t1 MTR_8g105170 22.330 206 155 2 363 564 328 532 1.20e-11 68.6
MS.gene054731.t1 MTR_4g074390 21.607 361 249 6 247 595 219 557 1.06e-12 71.6
MS.gene054731.t1 MTR_4g074390 21.739 345 247 8 228 565 166 494 5.04e-11 66.2
MS.gene054731.t1 MTR_7g011500 21.091 275 201 4 338 612 223 481 1.07e-12 72.0
MS.gene054731.t1 MTR_7g011500 24.413 213 147 4 425 634 202 403 1.60e-11 68.2
MS.gene054731.t1 MTR_7g011500 24.627 268 188 6 302 567 298 553 7.07e-11 65.9
MS.gene054731.t1 MTR_3g054110 20.796 452 306 8 237 649 116 554 1.07e-12 71.6
MS.gene054731.t1 MTR_7g076550 25.234 214 150 4 375 583 172 380 1.50e-12 70.9
MS.gene054731.t1 MTR_4g091600 25.726 241 133 6 384 583 192 427 1.61e-12 71.2
MS.gene054731.t1 MTR_1g108270 20.973 329 241 5 244 567 172 486 1.92e-12 70.5
MS.gene054731.t1 MTR_7g103600 24.309 362 251 7 221 580 183 523 2.06e-12 70.9
MS.gene054731.t1 MTR_4g108060 23.134 402 267 12 227 595 165 557 2.13e-12 70.5
MS.gene054731.t1 MTR_1g045880 22.680 291 211 5 279 569 236 512 2.28e-12 70.5
MS.gene054731.t1 MTR_6g079250 22.261 283 204 6 302 581 80 349 2.30e-12 69.7
MS.gene054731.t1 MTR_5g090210 22.739 387 238 10 199 583 255 582 2.88e-12 70.5
MS.gene054731.t1 MTR_5g090210 23.232 396 244 9 191 583 384 722 2.95e-12 70.5
MS.gene054731.t1 MTR_6g079210 23.790 248 185 2 334 581 106 349 2.96e-12 69.3
MS.gene054731.t1 MTR_6g065540 24.648 284 196 7 302 581 248 517 3.37e-12 70.1
MS.gene054731.t1 MTR_2g103520 24.314 255 177 5 384 634 211 453 4.27e-12 70.1
MS.gene054731.t1 MTR_3g034990 22.085 566 359 19 18 572 11 505 4.28e-12 69.7
MS.gene054731.t1 MTR_5g045490 26.749 243 168 5 378 619 320 553 5.44e-12 69.3
MS.gene054731.t1 MTR_7g017570 20.779 385 240 12 314 650 158 525 6.42e-12 69.3
MS.gene054731.t1 MTR_6g078210 25.581 172 127 1 395 566 334 504 7.08e-12 68.9
MS.gene054731.t1 MTR_4g108060 21.330 361 250 6 247 595 85 423 8.42e-12 68.2
MS.gene054731.t1 MTR_4g108060 21.965 346 247 8 227 565 31 360 6.52e-11 65.5
MS.gene054731.t1 MTR_5g077930 22.185 302 218 8 284 582 212 499 8.66e-12 68.9
MS.gene054731.t1 MTR_6g079180 23.387 248 186 2 334 581 106 349 9.42e-12 67.8
MS.gene054731.t1 MTR_6g072030 23.304 339 231 11 254 583 251 569 1.23e-11 68.2
MS.gene054731.t1 MTR_7g073260 24.561 342 211 14 300 615 147 467 1.68e-11 67.8
MS.gene054731.t1 MTR_2g036080 23.629 237 173 4 384 620 185 413 1.77e-11 68.2
MS.gene054731.t1 MTR_0014s0250 21.176 425 242 12 223 572 89 495 2.03e-11 67.4
MS.gene054731.t1 MTR_6g471100 20.639 407 281 12 168 562 227 603 2.18e-11 67.8
MS.gene054731.t1 MTR_2g026645 22.741 321 228 8 247 562 166 471 2.19e-11 67.4
MS.gene054731.t1 MTR_1g033960 21.637 513 328 18 139 610 164 643 3.14e-11 67.0
MS.gene054731.t1 MTR_1g095880 23.451 226 163 3 415 639 283 499 5.13e-11 66.6
MS.gene054731.t1 MTR_7g067460 24.599 187 140 1 392 578 213 398 6.82e-11 65.9
MS.gene054731.t1 MTR_8g107050 24.631 203 146 2 384 583 166 364 7.32e-11 65.9
MS.gene054731.t1 MTR_8g107050 24.631 203 146 2 384 583 166 364 7.38e-11 65.9
MS.gene054731.t1 MTR_8g089700 23.607 377 250 11 225 587 148 500 9.67e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054731.t1 AT1G69290 61.350 652 225 8 29 671 24 657 0.0 808
MS.gene054731.t1 AT1G68980 57.395 622 240 6 54 668 12 615 0.0 721
MS.gene054731.t1 AT1G03100 35.608 337 208 6 341 671 460 793 2.24e-64 228
MS.gene054731.t1 AT3G09650 29.213 356 232 4 334 670 408 762 5.42e-44 169
MS.gene054731.t1 AT4G17616 21.471 666 392 16 55 645 34 643 1.08e-27 119
MS.gene054731.t1 AT5G64320 22.351 519 311 19 151 620 219 694 2.20e-18 90.1
MS.gene054731.t1 AT5G39710 23.040 421 295 13 168 583 153 549 1.07e-16 84.7
MS.gene054731.t1 AT3G54980 24.615 390 256 7 225 583 266 648 2.03e-16 84.0
MS.gene054731.t1 AT3G54980 24.615 390 256 7 225 583 266 648 2.03e-16 84.0
MS.gene054731.t1 AT1G62720 25.337 371 244 11 225 588 137 481 3.94e-16 82.0
MS.gene054731.t1 AT5G04810 23.358 411 270 9 254 630 475 874 5.90e-16 82.4
MS.gene054731.t1 AT1G64583 25.360 347 217 9 250 563 128 465 3.30e-15 79.3
MS.gene054731.t1 AT1G64583 24.333 300 212 8 354 651 82 368 3.30e-11 66.6
MS.gene054731.t1 AT1G64583 25.360 347 217 9 250 563 103 440 4.92e-15 78.6
MS.gene054731.t1 AT1G64583 24.415 299 211 8 355 651 58 343 5.46e-11 65.9
MS.gene054731.t1 AT1G62670 22.101 457 284 13 132 581 172 563 9.87e-15 78.2
MS.gene054731.t1 AT1G62670 25.962 208 148 4 397 603 135 337 7.58e-11 65.9
MS.gene054731.t1 AT1G62670 22.101 457 284 13 132 581 172 563 9.87e-15 78.2
MS.gene054731.t1 AT1G62670 25.962 208 148 4 397 603 135 337 7.58e-11 65.9
MS.gene054731.t1 AT1G64100 25.571 219 155 3 419 637 438 648 1.04e-14 78.6
MS.gene054731.t1 AT1G64100 24.771 218 156 2 396 613 209 418 2.10e-11 67.8
MS.gene054731.t1 AT1G64100 23.494 332 219 8 252 583 165 461 2.23e-11 67.8
MS.gene054731.t1 AT1G64100 25.854 205 144 3 419 623 438 634 1.12e-14 78.2
MS.gene054731.t1 AT1G64100 25.946 185 136 1 396 580 209 392 1.66e-11 67.8
MS.gene054731.t1 AT1G64100 23.494 332 219 8 252 583 165 461 2.72e-11 67.4
MS.gene054731.t1 AT1G64100 25.854 205 144 3 419 623 438 634 1.12e-14 78.2
MS.gene054731.t1 AT1G64100 25.946 185 136 1 396 580 209 392 1.66e-11 67.8
MS.gene054731.t1 AT1G64100 23.494 332 219 8 252 583 165 461 2.72e-11 67.4
MS.gene054731.t1 AT3G53700 22.139 402 283 10 225 620 187 564 2.14e-14 77.4
MS.gene054731.t1 AT1G12620 28.351 194 134 2 395 588 369 557 4.37e-14 76.3
MS.gene054731.t1 AT1G12620 22.254 355 258 6 237 587 116 456 2.22e-11 67.4
MS.gene054731.t1 AT5G38730 26.667 225 156 4 384 608 209 424 5.68e-14 75.9
MS.gene054731.t1 AT3G07290 23.124 493 330 16 93 572 248 704 6.61e-14 75.9
MS.gene054731.t1 AT5G61990 20.436 597 376 14 80 603 316 886 6.66e-14 75.9
MS.gene054731.t1 AT5G61990 22.711 273 193 5 302 570 280 538 2.26e-11 67.8
MS.gene054731.t1 AT1G19520 22.050 322 233 8 244 561 400 707 7.03e-14 75.5
MS.gene054731.t1 AT5G61400 23.767 223 163 2 364 583 185 403 8.48e-14 75.5
MS.gene054731.t1 AT4G11690 22.865 363 222 8 262 583 162 507 1.24e-13 74.7
MS.gene054731.t1 AT1G63080 25.616 203 144 2 384 583 211 409 1.66e-13 74.3
MS.gene054731.t1 AT1G63080 22.670 397 265 12 250 634 122 488 3.05e-11 67.0
MS.gene054731.t1 AT1G63080 25.616 203 144 2 384 583 211 409 1.66e-13 74.3
MS.gene054731.t1 AT1G63080 22.670 397 265 12 250 634 122 488 3.05e-11 67.0
MS.gene054731.t1 AT1G63080 25.616 203 144 2 384 583 211 409 1.66e-13 74.3
MS.gene054731.t1 AT1G63080 22.670 397 265 12 250 634 122 488 3.05e-11 67.0
MS.gene054731.t1 AT1G22960 24.691 405 262 15 203 588 310 690 1.77e-13 74.3
MS.gene054731.t1 AT1G22960 24.691 405 262 15 203 588 310 690 1.77e-13 74.3
MS.gene054731.t1 AT1G62930 21.930 456 286 13 132 581 171 562 2.28e-13 73.9
MS.gene054731.t1 AT1G62930 24.444 405 274 14 250 647 137 516 1.36e-11 68.2
MS.gene054731.t1 AT1G62680 25.641 195 135 3 387 575 132 322 2.40e-13 73.6
MS.gene054731.t1 AT1G62680 25.641 195 135 3 387 575 132 322 2.40e-13 73.6
MS.gene054731.t1 AT3G06920 22.275 211 161 2 384 594 572 779 3.37e-13 73.6
MS.gene054731.t1 AT3G06920 22.275 211 161 2 384 594 572 779 3.37e-13 73.6
MS.gene054731.t1 AT3G06920 22.275 211 161 2 384 594 558 765 3.52e-13 73.6
MS.gene054731.t1 AT1G12300 25.296 253 180 4 395 646 385 629 3.98e-13 73.2
MS.gene054731.t1 AT1G12300 23.188 276 196 6 367 638 252 515 1.30e-11 68.2
MS.gene054731.t1 AT1G12300 24.868 189 140 2 384 572 199 385 8.11e-11 65.9
MS.gene054731.t1 AT1G62910 22.296 453 276 14 144 589 186 569 4.74e-13 72.8
MS.gene054731.t1 AT1G62910 22.222 342 240 8 254 587 109 432 8.31e-12 68.9
MS.gene054731.t1 AT1G62910 22.067 358 228 9 225 580 151 459 6.79e-11 65.9
MS.gene054731.t1 AT1G63230 25.500 200 148 1 384 583 47 245 5.26e-13 71.2
MS.gene054731.t1 AT2G39230 23.333 390 257 10 225 581 272 652 5.40e-13 72.8
MS.gene054731.t1 AT2G39230 21.067 356 266 8 230 583 452 794 9.27e-11 65.9
MS.gene054731.t1 AT1G63330 26.087 230 161 4 364 589 272 496 7.41e-13 72.0
MS.gene054731.t1 AT1G63330 26.087 230 161 4 364 589 272 496 7.41e-13 72.0
MS.gene054731.t1 AT1G63330 26.087 230 161 4 364 589 272 496 7.41e-13 72.0
MS.gene054731.t1 AT1G63150 21.739 368 251 9 254 614 109 446 8.88e-13 72.0
MS.gene054731.t1 AT1G63150 23.881 402 273 15 238 629 127 505 8.87e-12 68.9
MS.gene054731.t1 AT1G63150 21.739 368 251 9 254 614 109 446 8.88e-13 72.0
MS.gene054731.t1 AT1G63150 23.881 402 273 15 238 629 127 505 8.87e-12 68.9
MS.gene054731.t1 AT3G16710 30.939 181 123 2 384 563 123 302 1.20e-12 71.2
MS.gene054731.t1 AT3G16710 22.400 375 246 11 225 565 116 479 5.56e-11 65.9
MS.gene054731.t1 AT3G16710 30.939 181 123 2 384 563 123 302 1.24e-12 70.9
MS.gene054731.t1 AT4G19440 23.810 399 259 10 244 608 359 746 1.50e-12 71.6
MS.gene054731.t1 AT4G19440 23.810 399 259 10 244 608 359 746 1.50e-12 71.6
MS.gene054731.t1 AT4G19440 23.810 399 259 10 244 608 359 746 1.50e-12 71.6
MS.gene054731.t1 AT4G19440 23.810 399 259 10 244 608 359 746 1.50e-12 71.6
MS.gene054731.t1 AT1G79080 27.273 165 119 1 394 557 227 391 1.54e-12 71.2
MS.gene054731.t1 AT1G12775 23.229 353 249 7 225 572 191 526 1.69e-12 71.2
MS.gene054731.t1 AT1G12775 23.111 225 165 3 367 587 252 472 1.06e-11 68.6
MS.gene054731.t1 AT5G41170 27.811 169 121 1 397 565 26 193 1.81e-12 70.5
MS.gene054731.t1 AT5G41170 27.811 169 121 1 397 565 126 293 2.06e-12 70.5
MS.gene054731.t1 AT1G63130 24.206 252 175 5 342 589 328 567 2.17e-12 70.9
MS.gene054731.t1 AT1G63130 26.761 213 147 4 380 587 222 430 1.06e-11 68.6
MS.gene054731.t1 AT1G63130 24.206 252 175 5 342 589 328 567 2.17e-12 70.9
MS.gene054731.t1 AT1G63130 26.761 213 147 4 380 587 222 430 1.06e-11 68.6
MS.gene054731.t1 AT3G09060 23.684 342 190 10 302 578 293 628 2.59e-12 70.5
MS.gene054731.t1 AT3G09060 23.684 342 190 10 302 578 293 628 2.59e-12 70.5
MS.gene054731.t1 AT5G41170 27.811 169 121 1 397 565 126 293 2.75e-12 70.1
MS.gene054731.t1 AT5G50280 22.989 261 194 1 367 627 333 586 3.32e-12 70.5
MS.gene054731.t1 AT4G11690 22.650 234 147 3 384 583 247 480 3.86e-12 69.7
MS.gene054731.t1 AT1G12700 21.882 425 291 9 225 638 191 585 4.21e-12 69.7
MS.gene054731.t1 AT1G12700 21.882 425 291 9 225 638 207 601 4.46e-12 69.7
MS.gene054731.t1 AT1G12700 21.882 425 291 9 225 638 207 601 4.46e-12 69.7
MS.gene054731.t1 AT1G63400 25.481 208 147 3 384 587 231 434 4.82e-12 69.7
MS.gene054731.t1 AT1G63400 23.261 417 289 15 238 647 129 521 6.15e-12 69.3
MS.gene054731.t1 AT1G63400 23.973 292 200 7 302 589 298 571 2.00e-11 67.4
MS.gene054731.t1 AT1G05670 20.253 395 266 10 225 583 279 660 5.47e-12 69.7
MS.gene054731.t1 AT1G05670 20.253 395 266 10 225 583 279 660 5.47e-12 69.7
MS.gene054731.t1 AT1G05670 20.253 395 266 10 225 583 279 660 5.47e-12 69.7
MS.gene054731.t1 AT1G62590 26.087 230 161 4 364 589 347 571 6.71e-12 69.3
MS.gene054731.t1 AT1G62590 26.087 230 161 4 364 589 347 571 6.71e-12 69.3
MS.gene054731.t1 AT5G28370 20.930 516 354 15 91 585 201 683 8.31e-12 68.9
MS.gene054731.t1 AT5G28460 20.930 516 354 15 91 585 201 683 8.79e-12 68.9
MS.gene054731.t1 AT5G02860 23.611 288 202 6 338 621 171 444 1.03e-11 68.9
MS.gene054731.t1 AT5G02860 23.611 288 202 6 338 621 171 444 1.03e-11 68.9
MS.gene054731.t1 AT5G02860 23.611 288 202 6 338 621 171 444 1.03e-11 68.9
MS.gene054731.t1 AT5G02860 23.611 288 202 6 338 621 171 444 1.03e-11 68.9
MS.gene054731.t1 AT5G02860 23.611 288 202 6 338 621 171 444 1.03e-11 68.9
MS.gene054731.t1 AT1G64580 22.863 468 308 12 168 602 63 510 1.16e-11 68.2
MS.gene054731.t1 AT4G26800 24.047 341 239 11 225 561 156 480 1.36e-11 67.8
MS.gene054731.t1 AT5G65560 23.767 223 126 5 416 638 326 504 1.72e-11 68.2
MS.gene054731.t1 AT5G65560 23.767 223 126 5 416 638 326 504 1.72e-11 68.2
MS.gene054731.t1 AT5G65560 23.767 223 126 5 416 638 326 504 1.72e-11 68.2
MS.gene054731.t1 AT3G61520 20.725 386 272 7 233 585 299 683 1.75e-11 68.2
MS.gene054731.t1 AT4G26800 24.047 341 239 11 225 561 81 405 2.97e-11 66.6
MS.gene054731.t1 AT4G26800 24.047 341 239 11 225 561 11 335 4.45e-11 65.5
MS.gene054731.t1 AT2G06000 20.611 393 275 11 256 639 96 460 4.94e-11 66.2
MS.gene054731.t1 AT2G06000 20.611 393 275 11 256 639 96 460 4.94e-11 66.2
MS.gene054731.t1 AT2G15630 20.430 372 277 9 225 595 188 541 4.99e-11 66.2
MS.gene054731.t1 AT1G62914 24.880 209 149 3 383 587 223 427 5.00e-11 66.2
MS.gene054731.t1 AT2G41720 20.485 371 271 9 260 627 317 666 5.18e-11 66.6
MS.gene054731.t1 AT2G41720 20.596 369 275 7 259 627 184 534 5.76e-11 66.2
MS.gene054731.t1 AT2G41720 20.485 371 271 9 260 627 317 666 6.62e-11 66.2
MS.gene054731.t1 AT3G49730 26.857 175 118 5 397 566 290 459 7.39e-11 65.9
MS.gene054731.t1 AT1G13040 20.173 347 263 4 239 583 160 494 9.08e-11 65.5
MS.gene054731.t1 AT1G13040 20.173 347 263 4 239 583 160 494 9.08e-11 65.5

Find 133 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTATTTATATGCACTTAA+AGG 0.150295 5.3:-33156728 MS.gene054731:CDS
GTGAAGTTGGGAGATGATTT+TGG 0.175161 5.3:+33157368 None:intergenic
TGGGAGGCTCTGTGGGTCTT+GGG 0.176003 5.3:-33156316 MS.gene054731:CDS
TTGGGAGGCTCTGTGGGTCT+TGG 0.177689 5.3:-33156317 MS.gene054731:CDS
TCTTAATAAAGGAAATATTA+AGG 0.223823 5.3:-33156486 MS.gene054731:CDS
CTCTTATTGCTTTCAAGAAT+TGG 0.227572 5.3:-33155557 MS.gene054731:CDS
GATTATTGAAGCTCAGAAAT+TGG 0.273337 5.3:-33156450 MS.gene054731:CDS
AGCTCAAAGTGGCCAGATTA+AGG 0.274692 5.3:-33155605 MS.gene054731:CDS
AGGGAATTAGTGAGGGGTTT+GGG 0.279615 5.3:+33157211 None:intergenic
GTTTGGAAATCGAGAAGGTT+AGG 0.302425 5.3:+33157106 None:intergenic
AAGCCTGATGTGTTTAGTTT+TGG 0.305298 5.3:-33156758 MS.gene054731:CDS
CTTAATAAAGGAAATATTAA+GGG 0.325087 5.3:-33156485 MS.gene054731:CDS
CCGAAACATATCCGTTAATC+AGG 0.336662 5.3:+33155859 None:intergenic
GAAGGATGGTATGGATTGTT+TGG 0.341563 5.3:+33157089 None:intergenic
AGTGAGTTTGGTGGTGAAGT+TGG 0.350441 5.3:+33157355 None:intergenic
GGTCATTCGGCTCAAATTGC+AGG 0.350988 5.3:+33155895 None:intergenic
AGCAAGGTTGTCATTGTTCC+TGG 0.352806 5.3:+33156952 None:intergenic
GTTGACGAGTCTATAGGTTT+TGG 0.353471 5.3:-33156410 MS.gene054731:CDS
GAGATGATGACAAAGAAGTT+TGG 0.353870 5.3:-33156550 MS.gene054731:CDS
GTTAAGGAATATCTTAATAA+AGG 0.354824 5.3:-33156497 MS.gene054731:CDS
AAGGGAATTAGTGAGGGGTT+TGG 0.361527 5.3:+33157210 None:intergenic
TGAAAATATTTGTTGATAAG+TGG 0.364460 5.3:-33155662 MS.gene054731:CDS
GGAATGCATCAACCAGATTC+TGG 0.365582 5.3:+33155751 None:intergenic
CTTATTTATATGCACTTAAA+GGG 0.372712 5.3:-33156727 MS.gene054731:CDS
GATCAAATTTGACCAGAATC+TGG 0.373436 5.3:-33155763 MS.gene054731:CDS
TCTTATTGCTTTCAAGAATT+GGG 0.377175 5.3:-33155556 MS.gene054731:CDS
TGGTCAAATTTGATCTCCTT+CGG 0.380909 5.3:+33155771 None:intergenic
GACTATAATGACAGGTTTGA+TGG 0.384239 5.3:-33156075 MS.gene054731:CDS
CATCAGTTTGAGAAGTGATA+AGG 0.386250 5.3:+33157281 None:intergenic
GGTGTGAGGGAGAGTGAGTT+TGG 0.390112 5.3:+33157343 None:intergenic
GAGAAGTGATAAGGGATTTG+TGG 0.393693 5.3:+33157290 None:intergenic
GACAAGTATGTCTGGTCATT+CGG 0.398593 5.3:+33155882 None:intergenic
CCTGATTAACGGATATGTTT+CGG 0.406581 5.3:-33155859 MS.gene054731:CDS
ATCAGTTTGAGAAGTGATAA+GGG 0.409925 5.3:+33157282 None:intergenic
TGTTTCTATCAAAGCATCAT+AGG 0.418085 5.3:+33156187 None:intergenic
ATTGAGAATGTCGGGAAGAG+AGG 0.418936 5.3:+33157174 None:intergenic
GGGAGGCTCTGTGGGTCTTG+GGG 0.421493 5.3:-33156315 MS.gene054731:CDS
AGAGAACTGTAGGGTTGCTT+TGG 0.424623 5.3:-33156909 MS.gene054731:CDS
CTTGATGAGATGAATGCTTT+GGG 0.425114 5.3:-33156335 MS.gene054731:CDS
GTGACGGAGATTAGTAGTTC+AGG 0.428125 5.3:-33156230 MS.gene054731:CDS
ACATACTTGTCCCTGATTAA+CGG 0.428702 5.3:-33155870 MS.gene054731:CDS
TCTTGATGAGATGAATGCTT+TGG 0.429748 5.3:-33156336 MS.gene054731:CDS
AGGGGAAATTTGAATAAAGA+AGG 0.436356 5.3:+33157433 None:intergenic
CTCTGTTTAGGGACATGAGA+GGG 0.436667 5.3:-33156130 MS.gene054731:CDS
TTTCTTAGATGAGGTGGTTG+AGG 0.437022 5.3:-33156024 MS.gene054731:CDS
GTGAGTTTGGTGGTGAAGTT+GGG 0.438916 5.3:+33157356 None:intergenic
AACTGCGGAGGCTACTCTAT+TGG 0.438932 5.3:-33156252 MS.gene054731:CDS
GGTCTATGTGCCGATCTTGA+AGG 0.443651 5.3:-33156294 MS.gene054731:CDS
GTTAATGCTTGTGTTAGTAT+TGG 0.447831 5.3:-33156383 MS.gene054731:CDS
GAGAATGATCTTGTTCTATA+CGG 0.450102 5.3:+33157502 None:intergenic
TCTTGCATCTTCTAGCCTCC+CGG 0.455695 5.3:+33155941 None:intergenic
GAATTAGTGAGGGGTTTGGG+TGG 0.457507 5.3:+33157214 None:intergenic
ATCAAAGTTGACGAGTCTAT+AGG 0.458638 5.3:-33156416 MS.gene054731:CDS
GTCTATTATTATCATCATCA+TGG 0.458980 5.3:+33157395 None:intergenic
TTTGGAAATCGAGAAGGTTA+GGG 0.459663 5.3:+33157107 None:intergenic
AATGCTTTGGGAGGCTCTGT+GGG 0.462220 5.3:-33156323 MS.gene054731:CDS
TTGAGGTAGGTACTCATGAT+TGG 0.467602 5.3:-33155992 MS.gene054731:CDS
GAGATACAAGCAAGCGATCA+TGG 0.470178 5.3:-33155640 MS.gene054731:CDS
CTGAAAGTCTTGACCGGACA+TGG 0.473408 5.3:+33156163 None:intergenic
CTTCTCAAACTGATGAAGCT+TGG 0.474009 5.3:-33157273 MS.gene054731:CDS
AGTGAGGGGTTTGGGTGGGA+AGG 0.478855 5.3:+33157219 None:intergenic
GGCTGCTTTCTTAGATGAGG+TGG 0.482382 5.3:-33156030 MS.gene054731:CDS
GGTTGCTTTGGATGACAAGC+TGG 0.482568 5.3:-33156897 MS.gene054731:CDS
ACTGATGCTGACAGAGTTGT+TGG 0.482584 5.3:-33156803 MS.gene054731:CDS
ATCAGGCTTAACACCAAGCT+TGG 0.483315 5.3:+33156772 None:intergenic
TGAAGAGTGGTGAGTTGTTG+AGG 0.483350 5.3:+33157322 None:intergenic
GCGTGCTCGTTGATATTGCC+AGG 0.487094 5.3:-33156970 MS.gene054731:CDS
AATCCAAAACTAAACACATC+AGG 0.490880 5.3:+33156755 None:intergenic
ATTGCTTTCAAGAATTGGGC+TGG 0.497828 5.3:-33155552 MS.gene054731:CDS
GAGAGGATAAGTGAATGAGA+AGG 0.498892 5.3:+33157191 None:intergenic
TGAAGGAATGAGTAAAGAGA+TGG 0.502955 5.3:+33157457 None:intergenic
GAACCATCGACCTGAGTTGA+TGG 0.506113 5.3:-33156051 MS.gene054731:CDS
TCATAGAAAGAAGGATGGTA+TGG 0.510589 5.3:+33157080 None:intergenic
ATGGATGTTATTGTTGTTGA+GGG 0.511744 5.3:+33157414 None:intergenic
AATTAGTGAGGGGTTTGGGT+GGG 0.514397 5.3:+33157215 None:intergenic
ATTTGAATAAAGAAGGTTGA+AGG 0.514669 5.3:+33157440 None:intergenic
TAATCAGGGACAAGTATGTC+TGG 0.515514 5.3:+33155874 None:intergenic
TGGTGAGTTGTTGAGGTGTG+AGG 0.518181 5.3:+33157329 None:intergenic
ACGCCGCCCCACGAATGAAA+AGG 0.518977 5.3:+33156989 None:intergenic
AGAGGATAAGTGAATGAGAA+GGG 0.520500 5.3:+33157192 None:intergenic
GAATGCTTTGGGAGGCTCTG+TGG 0.523683 5.3:-33156324 MS.gene054731:CDS
CATGGATGTTATTGTTGTTG+AGG 0.527901 5.3:+33157413 None:intergenic
TTGCAGTAAGCCTTCAAGAT+CGG 0.528610 5.3:+33156284 None:intergenic
TAGGGACATGAGAGGGGCGA+GGG 0.531432 5.3:-33156123 MS.gene054731:CDS
TAGTAGTTCAGGTCTAAAGT+TGG 0.534004 5.3:-33156219 MS.gene054731:CDS
CTCCCTTGACCATAGCATAC+AGG 0.534135 5.3:+33155730 None:intergenic
TTAGGGACATGAGAGGGGCG+AGG 0.535492 5.3:-33156124 MS.gene054731:CDS
GGTGAGTTGTTGAGGTGTGA+GGG 0.536286 5.3:+33157330 None:intergenic
AGATGGAGTTTCACTTGATG+AGG 0.536425 5.3:+33157474 None:intergenic
TGGCGGATTTCATAGAAAGA+AGG 0.536674 5.3:+33157071 None:intergenic
TTGATAGAAACATCCATGTC+CGG 0.542115 5.3:-33156176 MS.gene054731:CDS
GACTGACTGAAAGTCTTGAC+CGG 0.545313 5.3:+33156157 None:intergenic
CGATTCAAGATGAAAACAGC+AGG 0.550214 5.3:+33156829 None:intergenic
GAATGAGAAGGGAATTAGTG+AGG 0.558752 5.3:+33157203 None:intergenic
AGTTTCACTTGATGAGGAGG+AGG 0.559123 5.3:+33157480 None:intergenic
AATGAGAAGGGAATTAGTGA+GGG 0.561114 5.3:+33157204 None:intergenic
GGTTGAGGATCCTCGTATTG+AGG 0.566012 5.3:-33156009 MS.gene054731:CDS
GCATTGCAAGCAGAAACATC+AGG 0.569511 5.3:+33156863 None:intergenic
GTTGGAATTATGTCCAAGCT+TGG 0.570246 5.3:-33156785 MS.gene054731:CDS
ATTCCTGTATGCTATGGTCA+AGG 0.570553 5.3:-33155733 MS.gene054731:CDS
AGTTACTTGACTATAATGAC+AGG 0.571830 5.3:-33156083 MS.gene054731:CDS
TGGAGTTTCACTTGATGAGG+AGG 0.574099 5.3:+33157477 None:intergenic
GATGGCTGCTTTCTTAGATG+AGG 0.574433 5.3:-33156033 MS.gene054731:CDS
GGATTGTTTGGAAATCGAGA+AGG 0.577250 5.3:+33157101 None:intergenic
TGATGCATTCCTGTATGCTA+TGG 0.578318 5.3:-33155739 MS.gene054731:CDS
AAGAAAGCAGCCATCAACTC+AGG 0.583919 5.3:+33156041 None:intergenic
GAGGTTAAGAGAAAGTTAGC+GGG 0.585709 5.3:-33155801 MS.gene054731:CDS
TTCCTGTATGCTATGGTCAA+GGG 0.590606 5.3:-33155732 MS.gene054731:CDS
GTGGAGAGTTGTTTGAAGAG+TGG 0.596034 5.3:+33157309 None:intergenic
TTCAGGTCTAAAGTTGGACG+TGG 0.606771 5.3:-33156213 MS.gene054731:CDS
CAGCCATCAACTCAGGTCGA+TGG 0.606962 5.3:+33156048 None:intergenic
ATGAGAAGGGAATTAGTGAG+GGG 0.607671 5.3:+33157205 None:intergenic
TCTCTGTTTAGGGACATGAG+AGG 0.609112 5.3:-33156131 MS.gene054731:CDS
TGATGCTGTGATGCAAGTTG+TGG 0.610490 5.3:-33155700 MS.gene054731:CDS
GATGAGATGAATGCTTTGGG+AGG 0.611114 5.3:-33156332 MS.gene054731:CDS
GTGAGGGAGAGTGAGTTTGG+TGG 0.615790 5.3:+33157346 None:intergenic
CGAGGTTAAGAGAAAGTTAG+CGG 0.617178 5.3:-33155802 MS.gene054731:CDS
TGTAATGATGTTGTGGAACG+AGG 0.624533 5.3:-33155820 MS.gene054731:CDS
TCTGTTTAGGGACATGAGAG+GGG 0.625777 5.3:-33156129 MS.gene054731:CDS
GAGGATCCTCGTATTGAGGT+AGG 0.626054 5.3:-33156005 MS.gene054731:CDS
CGAAACATATCCGTTAATCA+GGG 0.628051 5.3:+33155860 None:intergenic
GGAGGCTACTCTATTGGTGA+CGG 0.634065 5.3:-33156246 MS.gene054731:CDS
CTGTATGCTATGGTCAAGGG+AGG 0.634721 5.3:-33155729 MS.gene054731:CDS
GGAAGGAATGATGAGTTGTG+AGG 0.642580 5.3:+33157236 None:intergenic
GAAGGAATGATGAGTTGTGA+GGG 0.644080 5.3:+33157237 None:intergenic
CAAAGAGAATCGAACTGCGG+AGG 0.652528 5.3:-33156264 MS.gene054731:CDS
AGGTTAAGAGAAAGTTAGCG+GGG 0.663255 5.3:-33155800 MS.gene054731:CDS
GGATTTCATAGAAAGAAGGA+TGG 0.669801 5.3:+33157075 None:intergenic
TGGATGTTATTGTTGTTGAG+GGG 0.683344 5.3:+33157415 None:intergenic
AAACAGGCAAGAAAGCAGCA+AGG 0.715035 5.3:+33156936 None:intergenic
GGTTAAGAGAAAGTTAGCGG+GGG 0.720845 5.3:-33155799 MS.gene054731:CDS
CTGCAAAGAGAATCGAACTG+CGG 0.726664 5.3:-33156267 MS.gene054731:CDS
TGAGTACCTACCTCAATACG+AGG 0.748508 5.3:+33155999 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAATAAAGGAAATATTAA+GGG - chr5.3:33156589-33156608 MS.gene054731:CDS 15.0%
!! TCTTAATAAAGGAAATATTA+AGG - chr5.3:33156588-33156607 MS.gene054731:CDS 15.0%
!!! CATTACATCAAAATATTTTT+CGG + chr5.3:33157241-33157260 None:intergenic 15.0%
!!! TTTTATTCTAATTTGATTAG+TGG - chr5.3:33156439-33156458 MS.gene054731:CDS 15.0%
!! CTTATTTATATGCACTTAAA+GGG - chr5.3:33156347-33156366 MS.gene054731:CDS 20.0%
!! GTTAAGGAATATCTTAATAA+AGG - chr5.3:33156577-33156596 MS.gene054731:CDS 20.0%
!!! TGAAAATATTTGTTGATAAG+TGG - chr5.3:33157412-33157431 MS.gene054731:CDS 20.0%
! ATTTGAATAAAGAAGGTTGA+AGG + chr5.3:33155637-33155656 None:intergenic 25.0%
! GAAAAAGAATGTCAGAAAAA+TGG - chr5.3:33157491-33157510 MS.gene054731:CDS 25.0%
! GCTTATTTATATGCACTTAA+AGG - chr5.3:33156346-33156365 MS.gene054731:CDS 25.0%
! GTCTATTATTATCATCATCA+TGG + chr5.3:33155682-33155701 None:intergenic 25.0%
! TCTTATTGCTTTCAAGAATT+GGG - chr5.3:33157518-33157537 MS.gene054731:CDS 25.0%
!!! ATTTTGATGTAATGATGTTG+TGG - chr5.3:33157247-33157266 MS.gene054731:CDS 25.0%
!!! CACTTTTTGTTTAGTAGTTA+AGG - chr5.3:33156561-33156580 MS.gene054731:CDS 25.0%
AATCCAAAACTAAACACATC+AGG + chr5.3:33156322-33156341 None:intergenic 30.0%
AGGGGAAATTTGAATAAAGA+AGG + chr5.3:33155644-33155663 None:intergenic 30.0%
AGTTACTTGACTATAATGAC+AGG - chr5.3:33156991-33157010 MS.gene054731:CDS 30.0%
ATCAGTTTGAGAAGTGATAA+GGG + chr5.3:33155795-33155814 None:intergenic 30.0%
CTCTTATTGCTTTCAAGAAT+TGG - chr5.3:33157517-33157536 MS.gene054731:CDS 30.0%
GAGAATGATCTTGTTCTATA+CGG + chr5.3:33155575-33155594 None:intergenic 30.0%
GATTATTGAAGCTCAGAAAT+TGG - chr5.3:33156624-33156643 MS.gene054731:CDS 30.0%
GTTAATGCTTGTGTTAGTAT+TGG - chr5.3:33156691-33156710 MS.gene054731:CDS 30.0%
! ATGCAAGAAGAACTTTTAGA+AGG - chr5.3:33157148-33157167 MS.gene054731:CDS 30.0%
! ATGGATGTTATTGTTGTTGA+GGG + chr5.3:33155663-33155682 None:intergenic 30.0%
! GACATTCTTTTTCCTTAATC+TGG + chr5.3:33157484-33157503 None:intergenic 30.0%
! TGTTTCTATCAAAGCATCAT+AGG + chr5.3:33156890-33156909 None:intergenic 30.0%
! TTTCATTCCTTTTCATTCGT+GGG - chr5.3:33156078-33156097 MS.gene054731:CDS 30.0%
! TTTTCATTCCTTTTCATTCG+TGG - chr5.3:33156077-33156096 MS.gene054731:CDS 30.0%
!! AAAGTTTTGATGCTTGGTTT+TGG - chr5.3:33156394-33156413 MS.gene054731:CDS 30.0%
!! GAGTTAAAAGTTTTGATGCT+TGG - chr5.3:33156388-33156407 MS.gene054731:CDS 30.0%
!! GTGTTTTTGAATGAGAAAGA+GGG + chr5.3:33155948-33155967 None:intergenic 30.0%
!! TGTTTTTGAAGAGAACTGTA+GGG - chr5.3:33156156-33156175 MS.gene054731:CDS 30.0%
!!! TCTTTTGAGATTGAGAATGT+CGG + chr5.3:33155912-33155931 None:intergenic 30.0%
AATGAGAAGGGAATTAGTGA+GGG + chr5.3:33155873-33155892 None:intergenic 35.0%
ACATACTTGTCCCTGATTAA+CGG - chr5.3:33157204-33157223 MS.gene054731:CDS 35.0%
AGAGGATAAGTGAATGAGAA+GGG + chr5.3:33155885-33155904 None:intergenic 35.0%
ATCAAAGTTGACGAGTCTAT+AGG - chr5.3:33156658-33156677 MS.gene054731:CDS 35.0%
ATCTGACAAGTGAAAAAGCA+GGG + chr5.3:33156039-33156058 None:intergenic 35.0%
CATCAGTTTGAGAAGTGATA+AGG + chr5.3:33155796-33155815 None:intergenic 35.0%
CGAAACATATCCGTTAATCA+GGG + chr5.3:33157217-33157236 None:intergenic 35.0%
CTACAGTTCTCTTCAAAAAC+AGG + chr5.3:33156157-33156176 None:intergenic 35.0%
CTTGATGAGATGAATGCTTT+GGG - chr5.3:33156739-33156758 MS.gene054731:CDS 35.0%
GACACATAAAAAGCTCAAAG+TGG - chr5.3:33157458-33157477 MS.gene054731:CDS 35.0%
GACTATAATGACAGGTTTGA+TGG - chr5.3:33156999-33157018 MS.gene054731:CDS 35.0%
GAGATGATGACAAAGAAGTT+TGG - chr5.3:33156524-33156543 MS.gene054731:CDS 35.0%
GATCAAATTTGACCAGAATC+TGG - chr5.3:33157311-33157330 MS.gene054731:CDS 35.0%
GGATTTCATAGAAAGAAGGA+TGG + chr5.3:33156002-33156021 None:intergenic 35.0%
TCATAGAAAGAAGGATGGTA+TGG + chr5.3:33155997-33156016 None:intergenic 35.0%
TCTTGATGAGATGAATGCTT+TGG - chr5.3:33156738-33156757 MS.gene054731:CDS 35.0%
TGAAGGAATGAGTAAAGAGA+TGG + chr5.3:33155620-33155639 None:intergenic 35.0%
TGGTCAAATTTGATCTCCTT+CGG + chr5.3:33157306-33157325 None:intergenic 35.0%
TTGATAGAAACATCCATGTC+CGG - chr5.3:33156898-33156917 MS.gene054731:CDS 35.0%
TTTGGAAATCGAGAAGGTTA+GGG + chr5.3:33155970-33155989 None:intergenic 35.0%
! AGTCAGTCTTTTCTCTGTTT+AGG - chr5.3:33156932-33156951 MS.gene054731:CDS 35.0%
! CATGGATGTTATTGTTGTTG+AGG + chr5.3:33155664-33155683 None:intergenic 35.0%
! CCTGATTAACGGATATGTTT+CGG - chr5.3:33157215-33157234 MS.gene054731:CDS 35.0%
! GTCAGTCTTTTCTCTGTTTA+GGG - chr5.3:33156933-33156952 MS.gene054731:CDS 35.0%
! TAGTAGTTCAGGTCTAAAGT+TGG - chr5.3:33156855-33156874 MS.gene054731:CDS 35.0%
! TGAAGTTGGGAGATGATTTT+GGG + chr5.3:33155708-33155727 None:intergenic 35.0%
! TGGATGTTATTGTTGTTGAG+GGG + chr5.3:33155662-33155681 None:intergenic 35.0%
! TTCATTCCTTTTCATTCGTG+GGG - chr5.3:33156079-33156098 MS.gene054731:CDS 35.0%
!! AAGCCTGATGTGTTTAGTTT+TGG - chr5.3:33156316-33156335 MS.gene054731:CDS 35.0%
!! GGTGTTTTTGAATGAGAAAG+AGG + chr5.3:33155949-33155968 None:intergenic 35.0%
!!! CTGCTGTTTTCATCTTGAAT+CGG - chr5.3:33156246-33156265 MS.gene054731:CDS 35.0%
!!! CTGTTTTTGAAGAGAACTGT+AGG - chr5.3:33156155-33156174 MS.gene054731:CDS 35.0%
!!! CTTTTGAGATTGAGAATGTC+GGG + chr5.3:33155911-33155930 None:intergenic 35.0%
AGATGGAGTTTCACTTGATG+AGG + chr5.3:33155603-33155622 None:intergenic 40.0%
AGGTTAAGAGAAAGTTAGCG+GGG - chr5.3:33157274-33157293 MS.gene054731:CDS 40.0%
ATGAGAAGGGAATTAGTGAG+GGG + chr5.3:33155872-33155891 None:intergenic 40.0%
ATTCCTGTATGCTATGGTCA+AGG - chr5.3:33157341-33157360 MS.gene054731:CDS 40.0%
ATTGCTTTCAAGAATTGGGC+TGG - chr5.3:33157522-33157541 MS.gene054731:CDS 40.0%
CCGAAACATATCCGTTAATC+AGG + chr5.3:33157218-33157237 None:intergenic 40.0%
CGAGGTTAAGAGAAAGTTAG+CGG - chr5.3:33157272-33157291 MS.gene054731:CDS 40.0%
CGATTCAAGATGAAAACAGC+AGG + chr5.3:33156248-33156267 None:intergenic 40.0%
CTTCTCAAACTGATGAAGCT+TGG - chr5.3:33155801-33155820 MS.gene054731:CDS 40.0%
GAAGGAATGATGAGTTGTGA+GGG + chr5.3:33155840-33155859 None:intergenic 40.0%
GAATGAGAAGGGAATTAGTG+AGG + chr5.3:33155874-33155893 None:intergenic 40.0%
GACAAGTATGTCTGGTCATT+CGG + chr5.3:33157195-33157214 None:intergenic 40.0%
GAGAAGTGATAAGGGATTTG+TGG + chr5.3:33155787-33155806 None:intergenic 40.0%
GAGAGGATAAGTGAATGAGA+AGG + chr5.3:33155886-33155905 None:intergenic 40.0%
GAGGTTAAGAGAAAGTTAGC+GGG - chr5.3:33157273-33157292 MS.gene054731:CDS 40.0%
GATCTGACAAGTGAAAAAGC+AGG + chr5.3:33156040-33156059 None:intergenic 40.0%
GGATTGTTTGGAAATCGAGA+AGG + chr5.3:33155976-33155995 None:intergenic 40.0%
GTTGGAATTATGTCCAAGCT+TGG - chr5.3:33156289-33156308 MS.gene054731:CDS 40.0%
GTTTGGAAATCGAGAAGGTT+AGG + chr5.3:33155971-33155990 None:intergenic 40.0%
TAATCAGGGACAAGTATGTC+TGG + chr5.3:33157203-33157222 None:intergenic 40.0%
TCTGACAAGTGAAAAAGCAG+GGG + chr5.3:33156038-33156057 None:intergenic 40.0%
TGATGCATTCCTGTATGCTA+TGG - chr5.3:33157335-33157354 MS.gene054731:CDS 40.0%
TGTAATGATGTTGTGGAACG+AGG - chr5.3:33157254-33157273 MS.gene054731:CDS 40.0%
TTCCTGTATGCTATGGTCAA+GGG - chr5.3:33157342-33157361 MS.gene054731:CDS 40.0%
TTGAGGTAGGTACTCATGAT+TGG - chr5.3:33157082-33157101 MS.gene054731:CDS 40.0%
TTGCAGTAAGCCTTCAAGAT+CGG + chr5.3:33156793-33156812 None:intergenic 40.0%
TTTCTTAGATGAGGTGGTTG+AGG - chr5.3:33157050-33157069 MS.gene054731:CDS 40.0%
! GAAGGATGGTATGGATTGTT+TGG + chr5.3:33155988-33156007 None:intergenic 40.0%
! GAAGTTGGGAGATGATTTTG+GGG + chr5.3:33155707-33155726 None:intergenic 40.0%
! GTGAAGTTGGGAGATGATTT+TGG + chr5.3:33155709-33155728 None:intergenic 40.0%
! GTTGACGAGTCTATAGGTTT+TGG - chr5.3:33156664-33156683 MS.gene054731:CDS 40.0%
!! ATCCACGCTTTTTGTAAAGC+CGG - chr5.3:33157114-33157133 MS.gene054731:CDS 40.0%
!! TGGCGGATTTCATAGAAAGA+AGG + chr5.3:33156006-33156025 None:intergenic 40.0%
AAACAGGCAAGAAAGCAGCA+AGG + chr5.3:33156141-33156160 None:intergenic 45.0%
AAGAAAGCAGCCATCAACTC+AGG + chr5.3:33157036-33157055 None:intergenic 45.0%
AAGGGAATTAGTGAGGGGTT+TGG + chr5.3:33155867-33155886 None:intergenic 45.0%
AATTAGTGAGGGGTTTGGGT+GGG + chr5.3:33155862-33155881 None:intergenic 45.0%
ACTGATGCTGACAGAGTTGT+TGG - chr5.3:33156271-33156290 MS.gene054731:CDS 45.0%
AGAGAACTGTAGGGTTGCTT+TGG - chr5.3:33156165-33156184 MS.gene054731:CDS 45.0%
AGCAAGGTTGTCATTGTTCC+TGG + chr5.3:33156125-33156144 None:intergenic 45.0%
AGCTCAAAGTGGCCAGATTA+AGG - chr5.3:33157469-33157488 MS.gene054731:CDS 45.0%
AGGGAATTAGTGAGGGGTTT+GGG + chr5.3:33155866-33155885 None:intergenic 45.0%
AGTTTCACTTGATGAGGAGG+AGG + chr5.3:33155597-33155616 None:intergenic 45.0%
ATCAGGCTTAACACCAAGCT+TGG + chr5.3:33156305-33156324 None:intergenic 45.0%
ATTGAGAATGTCGGGAAGAG+AGG + chr5.3:33155903-33155922 None:intergenic 45.0%
CTCTGTTTAGGGACATGAGA+GGG - chr5.3:33156944-33156963 MS.gene054731:CDS 45.0%
CTGACAAGTGAAAAAGCAGG+GGG + chr5.3:33156037-33156056 None:intergenic 45.0%
CTGCAAAGAGAATCGAACTG+CGG - chr5.3:33156807-33156826 MS.gene054731:CDS 45.0%
GACTGACTGAAAGTCTTGAC+CGG + chr5.3:33156920-33156939 None:intergenic 45.0%
GAGATACAAGCAAGCGATCA+TGG - chr5.3:33157434-33157453 MS.gene054731:CDS 45.0%
GCATTGCAAGCAGAAACATC+AGG + chr5.3:33156214-33156233 None:intergenic 45.0%
GGAAGGAATGATGAGTTGTG+AGG + chr5.3:33155841-33155860 None:intergenic 45.0%
GGAATGCATCAACCAGATTC+TGG + chr5.3:33157326-33157345 None:intergenic 45.0%
GGTTAAGAGAAAGTTAGCGG+GGG - chr5.3:33157275-33157294 MS.gene054731:CDS 45.0%
GTGGAGAGTTGTTTGAAGAG+TGG + chr5.3:33155768-33155787 None:intergenic 45.0%
TCTCTGTTTAGGGACATGAG+AGG - chr5.3:33156943-33156962 MS.gene054731:CDS 45.0%
TCTGTTTAGGGACATGAGAG+GGG - chr5.3:33156945-33156964 MS.gene054731:CDS 45.0%
TGAAGAGTGGTGAGTTGTTG+AGG + chr5.3:33155755-33155774 None:intergenic 45.0%
TGAGTACCTACCTCAATACG+AGG + chr5.3:33157078-33157097 None:intergenic 45.0%
TGATGCTGTGATGCAAGTTG+TGG - chr5.3:33157374-33157393 MS.gene054731:CDS 45.0%
TGGAGTTTCACTTGATGAGG+AGG + chr5.3:33155600-33155619 None:intergenic 45.0%
! ATTCCTTTTCATTCGTGGGG+CGG - chr5.3:33156082-33156101 MS.gene054731:CDS 45.0%
! GATGAGATGAATGCTTTGGG+AGG - chr5.3:33156742-33156761 MS.gene054731:CDS 45.0%
! GATGGCTGCTTTCTTAGATG+AGG - chr5.3:33157041-33157060 MS.gene054731:CDS 45.0%
! GTGACGGAGATTAGTAGTTC+AGG - chr5.3:33156844-33156863 MS.gene054731:CDS 45.0%
! TTCAGGTCTAAAGTTGGACG+TGG - chr5.3:33156861-33156880 MS.gene054731:CDS 45.0%
!! AGTGAGTTTGGTGGTGAAGT+TGG + chr5.3:33155722-33155741 None:intergenic 45.0%
!! GTGAGTTTGGTGGTGAAGTT+GGG + chr5.3:33155721-33155740 None:intergenic 45.0%
!! TCCACGCTTTTTGTAAAGCC+GGG - chr5.3:33157115-33157134 MS.gene054731:CDS 45.0%
AACTGCGGAGGCTACTCTAT+TGG - chr5.3:33156822-33156841 MS.gene054731:CDS 50.0%
AAGAGAAAGTTAGCGGGGGA+TGG - chr5.3:33157279-33157298 MS.gene054731:CDS 50.0%
AGAGAAAGTTAGCGGGGGAT+GGG - chr5.3:33157280-33157299 MS.gene054731:CDS 50.0%
CAAAGAGAATCGAACTGCGG+AGG - chr5.3:33156810-33156829 MS.gene054731:CDS 50.0%
CTCCCTTGACCATAGCATAC+AGG + chr5.3:33157347-33157366 None:intergenic 50.0%
CTGAAAGTCTTGACCGGACA+TGG + chr5.3:33156914-33156933 None:intergenic 50.0%
CTGTATGCTATGGTCAAGGG+AGG - chr5.3:33157345-33157364 MS.gene054731:CDS 50.0%
GAATTAGTGAGGGGTTTGGG+TGG + chr5.3:33155863-33155882 None:intergenic 50.0%
GAGGATCCTCGTATTGAGGT+AGG - chr5.3:33157069-33157088 MS.gene054731:CDS 50.0%
GGAGGCTACTCTATTGGTGA+CGG - chr5.3:33156828-33156847 MS.gene054731:CDS 50.0%
GGTCATTCGGCTCAAATTGC+AGG + chr5.3:33157182-33157201 None:intergenic 50.0%
GGTGAGTTGTTGAGGTGTGA+GGG + chr5.3:33155747-33155766 None:intergenic 50.0%
GGTTGAGGATCCTCGTATTG+AGG - chr5.3:33157065-33157084 MS.gene054731:CDS 50.0%
TCCCGGCTTTACAAAAAGCG+TGG + chr5.3:33157119-33157138 None:intergenic 50.0%
TCTTGCATCTTCTAGCCTCC+CGG + chr5.3:33157136-33157155 None:intergenic 50.0%
TGGTGAGTTGTTGAGGTGTG+AGG + chr5.3:33155748-33155767 None:intergenic 50.0%
! GAACCATCGACCTGAGTTGA+TGG - chr5.3:33157023-33157042 MS.gene054731:CDS 50.0%
! GGCTGCTTTCTTAGATGAGG+TGG - chr5.3:33157044-33157063 MS.gene054731:CDS 50.0%
! GGTCTATGTGCCGATCTTGA+AGG - chr5.3:33156780-33156799 MS.gene054731:CDS 50.0%
! GGTTGCTTTGGATGACAAGC+TGG - chr5.3:33156177-33156196 MS.gene054731:CDS 50.0%
!! AATGCTTTGGGAGGCTCTGT+GGG - chr5.3:33156751-33156770 MS.gene054731:CDS 50.0%
!! ACGCTTTTTGTAAAGCCGGG+AGG - chr5.3:33157118-33157137 MS.gene054731:CDS 50.0%
AAAAGCAGGGGGAGCAGTGT+TGG + chr5.3:33156026-33156045 None:intergenic 55.0%
CAGCCATCAACTCAGGTCGA+TGG + chr5.3:33157029-33157048 None:intergenic 55.0%
GCGTGCTCGTTGATATTGCC+AGG - chr5.3:33156104-33156123 MS.gene054731:CDS 55.0%
GGTGTGAGGGAGAGTGAGTT+TGG + chr5.3:33155734-33155753 None:intergenic 55.0%
GTGAGGGAGAGTGAGTTTGG+TGG + chr5.3:33155731-33155750 None:intergenic 55.0%
!! GAATGCTTTGGGAGGCTCTG+TGG - chr5.3:33156750-33156769 MS.gene054731:CDS 55.0%
ACGCCGCCCCACGAATGAAA+AGG + chr5.3:33156088-33156107 None:intergenic 60.0%
AGTGAGGGGTTTGGGTGGGA+AGG + chr5.3:33155858-33155877 None:intergenic 60.0%
TAGGGACATGAGAGGGGCGA+GGG - chr5.3:33156951-33156970 MS.gene054731:CDS 60.0%
TGGGAGGCTCTGTGGGTCTT+GGG - chr5.3:33156758-33156777 MS.gene054731:CDS 60.0%
TTAGGGACATGAGAGGGGCG+AGG - chr5.3:33156950-33156969 MS.gene054731:CDS 60.0%
TTGGGAGGCTCTGTGGGTCT+TGG - chr5.3:33156757-33156776 MS.gene054731:CDS 60.0%
GGGAGGCTCTGTGGGTCTTG+GGG - chr5.3:33156759-33156778 MS.gene054731:CDS 65.0%
! AGCAGGGGGAGCAGTGTTGG+CGG + chr5.3:33156023-33156042 None:intergenic 65.0%
GTTAGCGGGGGATGGGCCGA+AGG - chr5.3:33157287-33157306 MS.gene054731:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 33155539 33157557 33155539 ID=MS.gene054731
chr5.3 mRNA 33155539 33157557 33155539 ID=MS.gene054731.t1;Parent=MS.gene054731
chr5.3 exon 33155539 33157557 33155539 ID=MS.gene054731.t1.exon1;Parent=MS.gene054731.t1
chr5.3 CDS 33155539 33157557 33155539 ID=cds.MS.gene054731.t1;Parent=MS.gene054731.t1
Gene Sequence

>MS.gene054731

ATGGCGAAAACGCTTATGAAATTGAATGTTGTTCCGTATAGAACAAGATCATTCTCCTCCTCCTCATCAAGTGAAACTCCATCTCTTTACTCATTCCTTCAACCTTCTTTATTCAAATTTCCCCTCAACAACAATAACATCCATGATGATGATAATAATAGACAACCCCAAAATCATCTCCCAACTTCACCACCAAACTCACTCTCCCTCACACCTCAACAACTCACCACTCTTCAAACAACTCTCCACAAATCCCTTATCACTTCTCAAACTGATGAAGCTTGGAAATCTTTCAAAACCCTCACAACTCATCATTCCTTCCCACCCAAACCCCTCACTAATTCCCTTCTCATTCACTTATCCTCTCTTCCCGACATTCTCAATCTCAAAAGAGCTTTCGCTTCAACCCTCTTTCTCATTCAAAAACACCCTAACCTTCTCGATTTCCAAACAATCCATACCATCCTTCTTTCTATGAAATCCGCCAACACTGCTCCCCCTGCTTTTTCACTTGTCAGATCAATGTTGAAGCATCGTTTTTTCATTCCTTTTCATTCGTGGGGCGGCGTGCTCGTTGATATTGCCAGGAACAATGACAACCTTGCTGCTTTCTTGCCTGTTTTTGAAGAGAACTGTAGGGTTGCTTTGGATGACAAGCTGGATTTCATGATACCTGATGTTTCTGCTTGCAATGCTGCTCTTCAAGCCTGCTGTTTTCATCTTGAATCGGTAACTGATGCTGACAGAGTTGTTGGAATTATGTCCAAGCTTGGTGTTAAGCCTGATGTGTTTAGTTTTGGATTTCTTGCTTATTTATATGCACTTAAAGGGTTACAAGATATGATAGACGAGTTAAAAGTTTTGATGCTTGGTTTTGGCTACTCAAAAAACAGTAAATGTTTTTATTCTAATTTGATTAGTGGCTATGTTAAGTCTGCTAATTTAGCTTCTGTAGAGTCAACTATTCTCAGTAGTTTGAATGATAGAGATGATGACAAAGAAGTTTGGAATTTTGATAGAGACACTTTTTGTTTAGTAGTTAAGGAATATCTTAATAAAGGAAATATTAAGGGTTTAGCTAATTTGATTATTGAAGCTCAGAAATTGGAGTCTTCGAATATCAAAGTTGACGAGTCTATAGGTTTTGGTATTGTTAATGCTTGTGTTAGTATTGGATTATCAGACAAAGCGCATAGTATTCTTGATGAGATGAATGCTTTGGGAGGCTCTGTGGGTCTTGGGGTCTATGTGCCGATCTTGAAGGCTTACTGCAAAGAGAATCGAACTGCGGAGGCTACTCTATTGGTGACGGAGATTAGTAGTTCAGGTCTAAAGTTGGACGTGGAAACCTATGATGCTTTGATAGAAACATCCATGTCCGGTCAAGACTTTCAGTCAGTCTTTTCTCTGTTTAGGGACATGAGAGGGGCGAGGGTTCTTGATTTGAAAGTGAGTTACTTGACTATAATGACAGGTTTGATGGAGAACCATCGACCTGAGTTGATGGCTGCTTTCTTAGATGAGGTGGTTGAGGATCCTCGTATTGAGGTAGGTACTCATGATTGGAATTCTATAATCCACGCTTTTTGTAAAGCCGGGAGGCTAGAAGATGCAAGAAGAACTTTTAGAAGGATGACATTCCTGCAATTTGAGCCGAATGACCAGACATACTTGTCCCTGATTAACGGATATGTTTCGGCCGAAAAATATTTTGATGTAATGATGTTGTGGAACGAGGTTAAGAGAAAGTTAGCGGGGGATGGGCCGAAGGAGATCAAATTTGACCAGAATCTGGTTGATGCATTCCTGTATGCTATGGTCAAGGGAGGTTTCTTTGATGCTGTGATGCAAGTTGTGGAGAAATCTAAAGAGATGAAAATATTTGTTGATAAGTGGAGATACAAGCAAGCGATCATGGAGACACATAAAAAGCTCAAAGTGGCCAGATTAAGGAAAAAGAATGTCAGAAAAATGGAAGCTCTTATTGCTTTCAAGAATTGGGCTGGCTTAAATGCGTGA
Protein sequence

>MS.gene054731.t1

MAKTLMKLNVVPYRTRSFSSSSSSETPSLYSFLQPSLFKFPLNNNNIHDDDNNRQPQNHLPTSPPNSLSLTPQQLTTLQTTLHKSLITSQTDEAWKSFKTLTTHHSFPPKPLTNSLLIHLSSLPDILNLKRAFASTLFLIQKHPNLLDFQTIHTILLSMKSANTAPPAFSLVRSMLKHRFFIPFHSWGGVLVDIARNNDNLAAFLPVFEENCRVALDDKLDFMIPDVSACNAALQACCFHLESVTDADRVVGIMSKLGVKPDVFSFGFLAYLYALKGLQDMIDELKVLMLGFGYSKNSKCFYSNLISGYVKSANLASVESTILSSLNDRDDDKEVWNFDRDTFCLVVKEYLNKGNIKGLANLIIEAQKLESSNIKVDESIGFGIVNACVSIGLSDKAHSILDEMNALGGSVGLGVYVPILKAYCKENRTAEATLLVTEISSSGLKLDVETYDALIETSMSGQDFQSVFSLFRDMRGARVLDLKVSYLTIMTGLMENHRPELMAAFLDEVVEDPRIEVGTHDWNSIIHAFCKAGRLEDARRTFRRMTFLQFEPNDQTYLSLINGYVSAEKYFDVMMLWNEVKRKLAGDGPKEIKFDQNLVDAFLYAMVKGGFFDAVMQVVEKSKEMKIFVDKWRYKQAIMETHKKLKVARLRKKNVRKMEALIAFKNWAGLNA