Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055042.t1 | RHN46364.1 | 95.4 | 174 | 5 | 1 | 33 | 206 | 86 | 256 | 6.60E-80 | 307.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055042.t1 | A0A396H3R8 | 95.4 | 174 | 5 | 1 | 33 | 206 | 86 | 256 | 4.7e-80 | 307.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055042 | MS.gene08054 | 0.812194 | 4.79E-51 | -1.69E-46 |
| MS.gene055042 | MS.gene71493 | 0.800956 | 1.13E-48 | -1.69E-46 |
| MS.gene055042 | MS.gene78005 | 0.80269 | 4.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055042.t1 | MTR_0474s0020 | 66.522 | 230 | 24 | 2 | 33 | 257 | 86 | 267 | 1.65e-65 | 206 |
| MS.gene055042.t1 | MTR_0474s0020 | 71.503 | 193 | 7 | 1 | 33 | 225 | 86 | 230 | 5.59e-64 | 200 |
| MS.gene055042.t1 | MTR_7g066640 | 68.992 | 129 | 34 | 3 | 34 | 158 | 73 | 199 | 1.11e-34 | 124 |
| MS.gene055042.t1 | MTR_7g066620 | 72.222 | 72 | 17 | 1 | 189 | 257 | 20 | 91 | 1.96e-20 | 90.9 |
| MS.gene055042.t1 | MTR_7g066600 | 65.000 | 100 | 25 | 5 | 26 | 119 | 88 | 183 | 2.88e-17 | 77.8 |
| MS.gene055042.t1 | MTR_4g015930 | 40.426 | 141 | 83 | 1 | 112 | 252 | 131 | 270 | 5.73e-17 | 80.9 |
| MS.gene055042.t1 | MTR_4g015930 | 33.482 | 224 | 127 | 7 | 38 | 253 | 159 | 368 | 2.23e-11 | 64.3 |
| MS.gene055042.t1 | MTR_4g015930 | 40.426 | 141 | 83 | 1 | 112 | 252 | 131 | 270 | 6.99e-17 | 80.5 |
| MS.gene055042.t1 | MTR_4g015930 | 33.482 | 224 | 127 | 7 | 38 | 253 | 159 | 368 | 2.23e-11 | 64.3 |
| MS.gene055042.t1 | MTR_4g015930 | 40.426 | 141 | 83 | 1 | 112 | 252 | 131 | 270 | 8.36e-17 | 80.5 |
| MS.gene055042.t1 | MTR_4g015930 | 33.482 | 224 | 127 | 7 | 38 | 253 | 159 | 368 | 3.20e-11 | 63.9 |
| MS.gene055042.t1 | MTR_4g016870 | 41.221 | 131 | 73 | 4 | 134 | 263 | 104 | 231 | 1.73e-12 | 66.2 |
| MS.gene055042.t1 | MTR_2g069880 | 34.857 | 175 | 90 | 4 | 113 | 264 | 296 | 469 | 9.10e-12 | 65.1 |
| MS.gene055042.t1 | MTR_2g064580 | 34.857 | 175 | 90 | 4 | 113 | 264 | 309 | 482 | 9.72e-12 | 65.1 |
| MS.gene055042.t1 | MTR_4g016810 | 33.000 | 200 | 117 | 5 | 58 | 253 | 132 | 318 | 4.02e-11 | 63.2 |
| MS.gene055042.t1 | MTR_0017s0270 | 33.152 | 184 | 101 | 4 | 102 | 264 | 154 | 336 | 6.42e-11 | 62.0 |
| MS.gene055042.t1 | MTR_7g039330 | 34.857 | 175 | 103 | 4 | 78 | 252 | 396 | 559 | 7.78e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGACGTGGAAGACCATTT+AGG | 0.244814 | 7.4:-37681042 | None:intergenic |
| ATCTTTGAAGGAACTAGTTT+TGG | 0.252313 | 7.4:+37680593 | MS.gene055042:CDS |
| GACTTATCTTGGAACAATTT+AGG | 0.286885 | 7.4:+37681001 | MS.gene055042:CDS |
| TGGAGATCCTTGACTTATCT+TGG | 0.311010 | 7.4:+37680990 | MS.gene055042:CDS |
| TTCATCTAAGTTACTGCATC+TGG | 0.313678 | 7.4:+37680506 | MS.gene055042:CDS |
| GAGACGTGGAAGACCATTTA+GGG | 0.325733 | 7.4:-37681041 | None:intergenic |
| GATGGATTCAAGTTCGAGTT+TGG | 0.325831 | 7.4:-37680035 | None:intergenic |
| GATGGATAATGCTATTTGTT+TGG | 0.331067 | 7.4:+37680275 | MS.gene055042:CDS |
| TATATCAAAACTGAGGTCTT+TGG | 0.336096 | 7.4:+37680970 | MS.gene055042:CDS |
| AAATGATCCATTCAAGTTGT+TGG | 0.350755 | 7.4:-37681083 | None:intergenic |
| TTGACCAGTTCTTGTACAAT+TGG | 0.363852 | 7.4:-37680126 | None:intergenic |
| TTAAGTTCCAACAACTTGAA+TGG | 0.394894 | 7.4:+37681076 | MS.gene055042:CDS |
| TATTGCAGGGTTGAAACATC+TGG | 0.398677 | 7.4:+37680224 | MS.gene055042:intron |
| TGGATATTTGAGTAAGATAC+AGG | 0.403649 | 7.4:-37680106 | None:intergenic |
| CTCAAGAGTTTAAGTTTACA+AGG | 0.414839 | 7.4:+37680816 | MS.gene055042:CDS |
| AATGGATCATTTGATATTAG+TGG | 0.417350 | 7.4:+37681094 | MS.gene055042:CDS |
| ATATCCAATTGTACAAGAAC+TGG | 0.433929 | 7.4:+37680122 | MS.gene055042:CDS |
| ATGAAATCCTGCCTTCACTA+AGG | 0.439633 | 7.4:+37680562 | MS.gene055042:CDS |
| AAGGGGATTCACATCTTTGA+AGG | 0.444315 | 7.4:+37680581 | MS.gene055042:CDS |
| TTGTAAACTTAAACTCTTGA+GGG | 0.449163 | 7.4:-37680814 | None:intergenic |
| GATAATGCTATTTGTTTGGA+TGG | 0.463507 | 7.4:+37680279 | MS.gene055042:CDS |
| AATCCATCGATGGACGAACT+TGG | 0.474058 | 7.4:+37680050 | MS.gene055042:CDS |
| GGATGGCATTGGATTGGACT+CGG | 0.477919 | 7.4:+37680614 | MS.gene055042:CDS |
| CTTAAGTCTCTATCGTTAGC+TGG | 0.488734 | 7.4:+37680312 | MS.gene055042:CDS |
| CTTTGAAGTTGTTGTTTGAA+AGG | 0.497174 | 7.4:-37680755 | None:intergenic |
| ATACCATTGACAACAATCAT+TGG | 0.499198 | 7.4:-37679978 | None:intergenic |
| AGGGAAGAAAGTCTACTAAG+AGG | 0.511140 | 7.4:-37680795 | None:intergenic |
| TGAAATCCTGCCTTCACTAA+GGG | 0.512380 | 7.4:+37680563 | MS.gene055042:CDS |
| AGTTGTTGTTTGAAAGGTCA+AGG | 0.514326 | 7.4:-37680749 | None:intergenic |
| ACACCAATGATTGTTGTCAA+TGG | 0.531292 | 7.4:+37679975 | None:intergenic |
| TTGCCAAGTTCGTCCATCGA+TGG | 0.539094 | 7.4:-37680053 | None:intergenic |
| CTTGTAAACTTAAACTCTTG+AGG | 0.539285 | 7.4:-37680815 | None:intergenic |
| AATGATTGTTGTCAATGGTA+TGG | 0.540709 | 7.4:+37679980 | None:intergenic |
| TGAATCCCCTTAGTGAAGGC+AGG | 0.548028 | 7.4:-37680569 | None:intergenic |
| GTCGAATGCAACACCACCAC+AGG | 0.567424 | 7.4:+37680004 | MS.gene055042:CDS |
| ATCTAAGTTACTGCATCTGG+AGG | 0.578812 | 7.4:+37680509 | MS.gene055042:CDS |
| GAGTTTGGACACTCTTCCTG+TGG | 0.586281 | 7.4:-37680020 | None:intergenic |
| CTCGAACTTGAATCCATCGA+TGG | 0.609951 | 7.4:+37680040 | MS.gene055042:CDS |
| AATTGTTCCAAGATAAGTCA+AGG | 0.610391 | 7.4:-37680997 | None:intergenic |
| AATTGTACAAGAACTGGTCA+AGG | 0.613084 | 7.4:+37680128 | MS.gene055042:CDS |
| TCAAGAGTTTAAGTTTACAA+GGG | 0.616770 | 7.4:+37680817 | MS.gene055042:CDS |
| TTTGGACACTCTTCCTGTGG+TGG | 0.618481 | 7.4:-37680017 | None:intergenic |
| GAAATCCTGCCTTCACTAAG+GGG | 0.620134 | 7.4:+37680564 | MS.gene055042:CDS |
| TCTATCGTTAGCTGGATACT+CGG | 0.629604 | 7.4:+37680320 | MS.gene055042:CDS |
| GATGTGAATCCCCTTAGTGA+AGG | 0.645363 | 7.4:-37680573 | None:intergenic |
| AAGTCAAGAGATTTGAGACG+TGG | 0.660772 | 7.4:-37681055 | None:intergenic |
| TGGTAGATATATCAAAACTG+AGG | 0.721856 | 7.4:+37680963 | MS.gene055042:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAACAAATAATCAA+AGG | + | chr7.4:37680392-37680411 | MS.gene055042:intron | 10.0% |
| !! | ATTTACATATTATTATTGCA+GGG | + | chr7.4:37680211-37680230 | MS.gene055042:intron | 15.0% |
| !! | TAAGAAGAAAAAACTATATT+TGG | - | chr7.4:37680373-37680392 | None:intergenic | 15.0% |
| !! | TATTTACATATTATTATTGC+AGG | + | chr7.4:37680210-37680229 | MS.gene055042:intron | 15.0% |
| !! | AACATATAAACATACATGAT+TGG | - | chr7.4:37680944-37680963 | None:intergenic | 20.0% |
| !! | AATCATGTATGTTTATATGT+TGG | + | chr7.4:37680943-37680962 | MS.gene055042:intron | 20.0% |
| !! | GATTGATTGTTGTATAATTT+AGG | + | chr7.4:37680696-37680715 | MS.gene055042:intron | 20.0% |
| !!! | ATATGCTTTTCAAACAAAAA+CGG | - | chr7.4:37680915-37680934 | None:intergenic | 20.0% |
| !!! | CACTTTCCATTTTATTTTAT+CGG | + | chr7.4:37680660-37680679 | MS.gene055042:intron | 20.0% |
| ! | AATGGATCATTTGATATTAG+TGG | + | chr7.4:37681094-37681113 | MS.gene055042:CDS | 25.0% |
| ! | ATATAAACATACATGATTGG+TGG | - | chr7.4:37680941-37680960 | None:intergenic | 25.0% |
| ! | ATTCCTCCGATAAAATAAAA+TGG | - | chr7.4:37680669-37680688 | None:intergenic | 25.0% |
| ! | TTGTAAACTTAAACTCTTGA+GGG | - | chr7.4:37680817-37680836 | None:intergenic | 25.0% |
| !! | AGTATATTTTCATCCCTAAA+TGG | + | chr7.4:37681028-37681047 | MS.gene055042:CDS | 25.0% |
| !! | TCAAGAGTTTAAGTTTACAA+GGG | + | chr7.4:37680817-37680836 | MS.gene055042:CDS | 25.0% |
| !!! | GTTTTTGTTTGAAAAGCATA+TGG | + | chr7.4:37680914-37680933 | MS.gene055042:intron | 25.0% |
| !!! | TTTCCATTTTATTTTATCGG+AGG | + | chr7.4:37680663-37680682 | MS.gene055042:intron | 25.0% |
| AATTGTTCCAAGATAAGTCA+AGG | - | chr7.4:37681000-37681019 | None:intergenic | 30.0% | |
| ATATCCAATTGTACAAGAAC+TGG | + | chr7.4:37680122-37680141 | MS.gene055042:CDS | 30.0% | |
| CAAAACTGTCTGAAAAACTA+AGG | - | chr7.4:37680478-37680497 | None:intergenic | 30.0% | |
| CTTGTAAACTTAAACTCTTG+AGG | - | chr7.4:37680818-37680837 | None:intergenic | 30.0% | |
| GACTTATCTTGGAACAATTT+AGG | + | chr7.4:37681001-37681020 | MS.gene055042:CDS | 30.0% | |
| GTACAAATATGTATTAGTGC+TGG | - | chr7.4:37680456-37680475 | None:intergenic | 30.0% | |
| TATATCAAAACTGAGGTCTT+TGG | + | chr7.4:37680970-37680989 | MS.gene055042:CDS | 30.0% | |
| TGGATATTTGAGTAAGATAC+AGG | - | chr7.4:37680109-37680128 | None:intergenic | 30.0% | |
| TGGTAGATATATCAAAACTG+AGG | + | chr7.4:37680963-37680982 | MS.gene055042:intron | 30.0% | |
| TTAAGTTCCAACAACTTGAA+TGG | + | chr7.4:37681076-37681095 | MS.gene055042:CDS | 30.0% | |
| ! | CTCAAGAGTTTAAGTTTACA+AGG | + | chr7.4:37680816-37680835 | MS.gene055042:CDS | 30.0% |
| ! | GATAATGCTATTTGTTTGGA+TGG | + | chr7.4:37680279-37680298 | MS.gene055042:CDS | 30.0% |
| ! | GATGGATAATGCTATTTGTT+TGG | + | chr7.4:37680275-37680294 | MS.gene055042:CDS | 30.0% |
| !! | AAATGATCCATTCAAGTTGT+TGG | - | chr7.4:37681086-37681105 | None:intergenic | 30.0% |
| !! | ATCTTTGAAGGAACTAGTTT+TGG | + | chr7.4:37680593-37680612 | MS.gene055042:CDS | 30.0% |
| !! | CTTAGTTTTTCAGACAGTTT+TGG | + | chr7.4:37680476-37680495 | MS.gene055042:intron | 30.0% |
| !! | CTTTGAAGTTGTTGTTTGAA+AGG | - | chr7.4:37680758-37680777 | None:intergenic | 30.0% |
| !!! | CTCTCATGATAGTTTTATGA+TGG | + | chr7.4:37680257-37680276 | MS.gene055042:CDS | 30.0% |
| AATTGTACAAGAACTGGTCA+AGG | + | chr7.4:37680128-37680147 | MS.gene055042:CDS | 35.0% | |
| TTCATCTAAGTTACTGCATC+TGG | + | chr7.4:37680506-37680525 | MS.gene055042:CDS | 35.0% | |
| TTGACCAGTTCTTGTACAAT+TGG | - | chr7.4:37680129-37680148 | None:intergenic | 35.0% | |
| !! | AGTTGTTGTTTGAAAGGTCA+AGG | - | chr7.4:37680752-37680771 | None:intergenic | 35.0% |
| !! | CTGGTTAATGAATTTGCTGA+AGG | - | chr7.4:37680437-37680456 | None:intergenic | 35.0% |
| !!! | TTGAAGGAACTAGTTTTGGA+TGG | + | chr7.4:37680597-37680616 | MS.gene055042:CDS | 35.0% |
| AAGGGGATTCACATCTTTGA+AGG | + | chr7.4:37680581-37680600 | MS.gene055042:CDS | 40.0% | |
| AGGGAAGAAAGTCTACTAAG+AGG | - | chr7.4:37680798-37680817 | None:intergenic | 40.0% | |
| ATCTAAGTTACTGCATCTGG+AGG | + | chr7.4:37680509-37680528 | MS.gene055042:CDS | 40.0% | |
| ATGAAATCCTGCCTTCACTA+AGG | + | chr7.4:37680562-37680581 | MS.gene055042:CDS | 40.0% | |
| CAACACTTCATTACACAGTG+AGG | + | chr7.4:37680347-37680366 | MS.gene055042:intron | 40.0% | |
| CTTAAGTCTCTATCGTTAGC+TGG | + | chr7.4:37680312-37680331 | MS.gene055042:CDS | 40.0% | |
| TATTGCAGGGTTGAAACATC+TGG | + | chr7.4:37680224-37680243 | MS.gene055042:intron | 40.0% | |
| TCTATCGTTAGCTGGATACT+CGG | + | chr7.4:37680320-37680339 | MS.gene055042:CDS | 40.0% | |
| TGAAATCCTGCCTTCACTAA+GGG | + | chr7.4:37680563-37680582 | MS.gene055042:CDS | 40.0% | |
| TGGAGATCCTTGACTTATCT+TGG | + | chr7.4:37680990-37681009 | MS.gene055042:CDS | 40.0% | |
| ! | AAGTCAAGAGATTTGAGACG+TGG | - | chr7.4:37681058-37681077 | None:intergenic | 40.0% |
| ! | GACTCGGATCTTCATTTTCA+AGG | + | chr7.4:37680630-37680649 | MS.gene055042:CDS | 40.0% |
| ! | GATGGATTCAAGTTCGAGTT+TGG | - | chr7.4:37680038-37680057 | None:intergenic | 40.0% |
| !!! | AGTTTTGGATGGCATTGGAT+TGG | + | chr7.4:37680608-37680627 | MS.gene055042:CDS | 40.0% |
| !!! | GAACTAGTTTTGGATGGCAT+TGG | + | chr7.4:37680603-37680622 | MS.gene055042:CDS | 40.0% |
| AATCCATCGATGGACGAACT+TGG | + | chr7.4:37680050-37680069 | MS.gene055042:CDS | 45.0% | |
| CTCGAACTTGAATCCATCGA+TGG | + | chr7.4:37680040-37680059 | MS.gene055042:CDS | 45.0% | |
| GAAATCCTGCCTTCACTAAG+GGG | + | chr7.4:37680564-37680583 | MS.gene055042:CDS | 45.0% | |
| GAGACGTGGAAGACCATTTA+GGG | - | chr7.4:37681044-37681063 | None:intergenic | 45.0% | |
| GATGTGAATCCCCTTAGTGA+AGG | - | chr7.4:37680576-37680595 | None:intergenic | 45.0% | |
| TGAGACGTGGAAGACCATTT+AGG | - | chr7.4:37681045-37681064 | None:intergenic | 45.0% | |
| GAGTTTGGACACTCTTCCTG+TGG | - | chr7.4:37680023-37680042 | None:intergenic | 50.0% | |
| TGAATCCCCTTAGTGAAGGC+AGG | - | chr7.4:37680572-37680591 | None:intergenic | 50.0% | |
| TTGCCAAGTTCGTCCATCGA+TGG | - | chr7.4:37680056-37680075 | None:intergenic | 50.0% | |
| TTTGGACACTCTTCCTGTGG+TGG | - | chr7.4:37680020-37680039 | None:intergenic | 50.0% | |
| ! | GGATGGCATTGGATTGGACT+CGG | + | chr7.4:37680614-37680633 | MS.gene055042:CDS | 50.0% |
| GTCGAATGCAACACCACCAC+AGG | + | chr7.4:37680004-37680023 | MS.gene055042:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 37679981 | 37681144 | 37679981 | ID=MS.gene055042 |
| chr7.4 | mRNA | 37679981 | 37681144 | 37679981 | ID=MS.gene055042.t1;Parent=MS.gene055042 |
| chr7.4 | exon | 37679981 | 37680149 | 37679981 | ID=MS.gene055042.t1.exon1;Parent=MS.gene055042.t1 |
| chr7.4 | CDS | 37679981 | 37680149 | 37679981 | ID=cds.MS.gene055042.t1;Parent=MS.gene055042.t1 |
| chr7.4 | exon | 37680232 | 37680363 | 37680232 | ID=MS.gene055042.t1.exon2;Parent=MS.gene055042.t1 |
| chr7.4 | CDS | 37680232 | 37680363 | 37680232 | ID=cds.MS.gene055042.t1;Parent=MS.gene055042.t1 |
| chr7.4 | exon | 37680481 | 37680651 | 37680481 | ID=MS.gene055042.t1.exon3;Parent=MS.gene055042.t1 |
| chr7.4 | CDS | 37680481 | 37680651 | 37680481 | ID=cds.MS.gene055042.t1;Parent=MS.gene055042.t1 |
| chr7.4 | exon | 37680718 | 37680867 | 37680718 | ID=MS.gene055042.t1.exon4;Parent=MS.gene055042.t1 |
| chr7.4 | CDS | 37680718 | 37680867 | 37680718 | ID=cds.MS.gene055042.t1;Parent=MS.gene055042.t1 |
| chr7.4 | exon | 37680969 | 37681144 | 37680969 | ID=MS.gene055042.t1.exon5;Parent=MS.gene055042.t1 |
| chr7.4 | CDS | 37680969 | 37681144 | 37680969 | ID=cds.MS.gene055042.t1;Parent=MS.gene055042.t1 |
| Gene Sequence |
| Protein sequence |
