AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056048

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056048.t1 MTR_1g083070 98.413 126 2 0 1 126 1 126 4.07e-93 264
MS.gene056048.t1 MTR_1g088860 72.632 95 26 0 4 98 36 130 8.68e-50 157
MS.gene056048.t1 MTR_0392s0020 68.687 99 31 0 4 102 18 116 7.30e-48 150
MS.gene056048.t1 MTR_4g119500 71.579 95 27 0 4 98 22 116 7.16e-47 147
MS.gene056048.t1 MTR_4g133938 55.752 113 44 1 6 112 21 133 9.62e-42 135
MS.gene056048.t1 MTR_2g026710 63.043 92 34 0 4 95 17 108 1.01e-39 130
MS.gene056048.t1 MTR_3g058980 60.870 92 36 0 4 95 27 118 7.75e-39 129
MS.gene056048.t1 MTR_8g093920 59.794 97 39 0 4 100 40 136 8.25e-39 130
MS.gene056048.t1 MTR_5g095740 60.870 92 36 0 4 95 23 114 2.55e-38 127
MS.gene056048.t1 MTR_8g091720 60.870 92 36 0 4 95 28 119 4.51e-38 127
MS.gene056048.t1 MTR_7g061270 46.667 120 64 0 4 123 26 145 1.55e-37 125
MS.gene056048.t1 MTR_7g100650 46.667 120 64 0 4 123 26 145 2.43e-37 125
MS.gene056048.t1 MTR_1g072790 56.522 92 40 0 4 95 29 120 3.24e-36 122
MS.gene056048.t1 MTR_4g133952 51.087 92 45 0 4 95 58 149 3.13e-33 116
MS.gene056048.t1 MTR_1g039040 51.087 92 45 0 4 95 6 97 2.18e-31 110
MS.gene056048.t1 MTR_5g095900 43.564 101 55 1 1 101 13 111 7.82e-23 88.2
MS.gene056048.t1 MTR_1g029070 41.379 87 51 0 9 95 57 143 5.06e-20 80.9
MS.gene056048.t1 MTR_1g029100 35.000 80 52 0 9 88 7 86 3.12e-17 71.2
MS.gene056048.t1 MTR_2g056000 35.443 79 51 0 6 84 43 121 1.32e-15 69.7
MS.gene056048.t1 MTR_4g112380 31.646 79 54 0 6 84 13 91 1.27e-13 63.9
MS.gene056048.t1 MTR_1g028480 42.105 57 33 0 39 95 24 80 2.45e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056048.t1 AT1G09030 63.492 126 41 2 1 122 1 125 5.39e-51 158
MS.gene056048.t1 AT2G47810 68.421 95 30 0 1 95 48 142 1.76e-45 145
MS.gene056048.t1 AT5G47640 64.130 92 33 0 4 95 27 118 1.90e-40 133
MS.gene056048.t1 AT4G14540 60.870 92 36 0 4 95 21 112 2.70e-39 129
MS.gene056048.t1 AT2G13570 59.000 100 41 0 4 103 36 135 8.87e-39 130
MS.gene056048.t1 AT2G38880 52.212 113 50 1 4 116 21 129 7.16e-38 125
MS.gene056048.t1 AT2G38880 52.212 113 50 1 4 116 21 129 8.79e-38 125
MS.gene056048.t1 AT2G38880 52.212 113 50 1 4 116 21 129 8.79e-38 125
MS.gene056048.t1 AT2G38880 52.212 113 50 1 4 116 21 129 8.79e-38 125
MS.gene056048.t1 AT2G38880 58.242 91 38 0 4 94 21 111 1.00e-37 124
MS.gene056048.t1 AT2G38880 57.609 92 39 0 4 95 21 112 1.28e-37 124
MS.gene056048.t1 AT2G37060 56.522 92 40 0 4 95 30 121 2.58e-37 125
MS.gene056048.t1 AT2G37060 56.522 92 40 0 4 95 30 121 2.58e-37 125
MS.gene056048.t1 AT2G37060 56.522 92 40 0 4 95 30 121 2.58e-37 125
MS.gene056048.t1 AT3G53340 56.044 91 40 0 4 94 29 119 3.02e-37 123
MS.gene056048.t1 AT3G53340 55.914 93 41 0 4 96 29 121 3.14e-37 124
MS.gene056048.t1 AT3G53340 55.914 93 41 0 4 96 29 121 3.14e-37 124
MS.gene056048.t1 AT3G53340 56.522 92 40 0 4 95 29 120 4.16e-37 124
MS.gene056048.t1 AT3G53340 56.522 92 40 0 4 95 29 120 4.16e-37 124
MS.gene056048.t1 AT2G38880 58.242 91 38 0 4 94 21 111 5.56e-37 123
MS.gene056048.t1 AT2G38880 58.242 91 38 0 4 94 21 111 5.56e-37 123
MS.gene056048.t1 AT2G38880 58.242 91 38 0 4 94 21 111 5.56e-37 123
MS.gene056048.t1 AT2G38880 58.242 91 38 0 4 94 21 111 5.75e-37 124
MS.gene056048.t1 AT2G38880 58.242 91 38 0 4 94 21 111 5.75e-37 124
MS.gene056048.t1 AT5G47670 51.087 92 45 0 4 95 29 120 1.55e-32 114
MS.gene056048.t1 AT5G47670 51.087 92 45 0 4 95 58 149 4.15e-32 113
MS.gene056048.t1 AT5G47670 51.087 92 45 0 4 95 58 149 4.15e-32 113
MS.gene056048.t1 AT1G21970 49.474 95 48 0 4 98 59 153 3.37e-31 111
MS.gene056048.t1 AT5G23090 36.709 79 50 0 6 84 13 91 1.70e-16 71.2
MS.gene056048.t1 AT5G23090 36.709 79 50 0 6 84 13 91 1.79e-16 71.2
MS.gene056048.t1 AT5G23090 36.709 79 50 0 6 84 13 91 1.79e-16 71.2
MS.gene056048.t1 AT5G23090 36.709 79 50 0 6 84 13 91 1.79e-16 71.2
MS.gene056048.t1 AT5G08190 32.911 79 53 0 6 84 13 91 1.31e-14 66.6
MS.gene056048.t1 AT5G08190 32.911 79 53 0 6 84 13 91 1.41e-14 66.6

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAATCGCAAGACAGTTAA+TGG 0.277173 1.1:-61478540 MS.gene056048:CDS
TGTTGGGCTTTGTGTTCGTT+AGG 0.349672 1.1:-61478507 MS.gene056048:CDS
TGACTACTATGTTTGGTACT+TGG 0.390007 1.1:+61478357 None:intergenic
TTACAGATGACTACTATGTT+TGG 0.403528 1.1:+61478350 None:intergenic
GACAACTATGCTGAAGCTAT+TGG 0.407603 1.1:-61478480 MS.gene056048:CDS
AGAATCGCAAGACAGTTAAT+GGG 0.432736 1.1:-61478539 MS.gene056048:CDS
GTTGGGCTTTGTGTTCGTTA+GGG 0.471790 1.1:-61478506 MS.gene056048:CDS
TATTCTACCCACATTCGCTA+TGG 0.488385 1.1:+61478683 None:intergenic
TAATGGGGATGACATCTGTT+GGG 0.508909 1.1:-61478523 MS.gene056048:CDS
AACGTTGCCCATAGCGAATG+TGG 0.514518 1.1:-61478691 MS.gene056048:CDS
GAGTTCATAAGTTTCGTGAC+AGG 0.522754 1.1:-61478588 MS.gene056048:CDS
ATTCTACCCACATTCGCTAT+GGG 0.529148 1.1:+61478684 None:intergenic
TTAATGGGGATGACATCTGT+TGG 0.554383 1.1:-61478524 MS.gene056048:CDS
TTTGAGATCTTTGCAGTGGG+AGG 0.587845 1.1:+61478645 None:intergenic
TAGCTAGAAATGAACGATGA+GGG 0.603562 1.1:-61478720 None:intergenic
ACTATGCTGAAGCTATTGGA+AGG 0.633037 1.1:-61478476 MS.gene056048:CDS
AGCATCTGACAAGTGTCACA+AGG 0.642405 1.1:-61478562 MS.gene056048:CDS
GTAGCTAGAAATGAACGATG+AGG 0.648734 1.1:-61478721 None:intergenic
ACGTTGCCCATAGCGAATGT+GGG 0.650019 1.1:-61478690 MS.gene056048:CDS
GAATCGCAAGACAGTTAATG+GGG 0.668357 1.1:-61478538 MS.gene056048:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTACAGATGACTACTATGTT+TGG + chr1.1:61478714-61478733 None:intergenic 30.0%
AGAATCGCAAGACAGTTAAT+GGG - chr1.1:61478522-61478541 MS.gene056048:CDS 35.0%
TGACTACTATGTTTGGTACT+TGG + chr1.1:61478707-61478726 None:intergenic 35.0%
!!! TCTTTTGAGATCTTTGCAGT+GGG + chr1.1:61478422-61478441 None:intergenic 35.0%
!!! TTCTTTTGAGATCTTTGCAG+TGG + chr1.1:61478423-61478442 None:intergenic 35.0%
ACTATGCTGAAGCTATTGGA+AGG - chr1.1:61478585-61478604 MS.gene056048:CDS 40.0%
ATTCTACCCACATTCGCTAT+GGG + chr1.1:61478380-61478399 None:intergenic 40.0%
GAATCGCAAGACAGTTAATG+GGG - chr1.1:61478523-61478542 MS.gene056048:CDS 40.0%
GACAACTATGCTGAAGCTAT+TGG - chr1.1:61478581-61478600 MS.gene056048:CDS 40.0%
GAGAATCGCAAGACAGTTAA+TGG - chr1.1:61478521-61478540 MS.gene056048:CDS 40.0%
GAGTTCATAAGTTTCGTGAC+AGG - chr1.1:61478473-61478492 MS.gene056048:CDS 40.0%
TAATGGGGATGACATCTGTT+GGG - chr1.1:61478538-61478557 MS.gene056048:CDS 40.0%
TATTCTACCCACATTCGCTA+TGG + chr1.1:61478381-61478400 None:intergenic 40.0%
TTAATGGGGATGACATCTGT+TGG - chr1.1:61478537-61478556 MS.gene056048:CDS 40.0%
AGCATCTGACAAGTGTCACA+AGG - chr1.1:61478499-61478518 MS.gene056048:CDS 45.0%
! GTTGGGCTTTGTGTTCGTTA+GGG - chr1.1:61478555-61478574 MS.gene056048:CDS 45.0%
! TGTTGGGCTTTGTGTTCGTT+AGG - chr1.1:61478554-61478573 MS.gene056048:CDS 45.0%
! TTTGAGATCTTTGCAGTGGG+AGG + chr1.1:61478419-61478438 None:intergenic 45.0%
AACGTTGCCCATAGCGAATG+TGG - chr1.1:61478370-61478389 MS.gene056048:CDS 50.0%
ACGTTGCCCATAGCGAATGT+GGG - chr1.1:61478371-61478390 MS.gene056048:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 61478350 61478733 61478350 ID=MS.gene056048
chr1.1 mRNA 61478350 61478733 61478350 ID=MS.gene056048.t1;Parent=MS.gene056048
chr1.1 exon 61478350 61478733 61478350 ID=MS.gene056048.t1.exon1;Parent=MS.gene056048.t1
chr1.1 CDS 61478350 61478733 61478350 ID=cds.MS.gene056048.t1;Parent=MS.gene056048.t1
Gene Sequence

>MS.gene056048

ATGAACGATGAGGGAGATAAAACGTTGCCCATAGCGAATGTGGGTAGAATAATGAAGCAAAATCTTCCTCCCACTGCAAAGATCTCAAAAGAAAGCAAACAGTTGATGCAAGAATGTGCGACAGAGTTCATAAGTTTCGTGACAGGTGAAGCATCTGACAAGTGTCACAAGGAGAATCGCAAGACAGTTAATGGGGATGACATCTGTTGGGCTTTGTGTTCGTTAGGGTTTGACAACTATGCTGAAGCTATTGGAAGGTACTTGTATAAATTCAGACAAGCTGAACTTGTTAGAATAAATCAGAACAAACTTCATGAAACTGCAAAAGACAAGTTTGAAGAAGATGCAACAAATCCAAGTACCAAACATAGTAGTCATCTGTAA
Protein sequence

>MS.gene056048.t1

MNDEGDKTLPIANVGRIMKQNLPPTAKISKESKQLMQECATEFISFVTGEASDKCHKENRKTVNGDDICWALCSLGFDNYAEAIGRYLYKFRQAELVRINQNKLHETAKDKFEEDATNPSTKHSSHL