AlfalfaGEDB | MS.gene056322

M. sativa cultivar XinJiangDaYe / MS.gene056322

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056322.t1	XP_003606738.2	94.8	270	13	1	1	270	141	409	4.50E-145	523.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056322.t1	Q9SYB9	60.0	270	78	6	1	266	147	390	1.7e-84	313.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056322.t1	G7JCH4	94.8	270	13	1	1	270	141	409	3.3e-145	523.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056321	MS.gene056322	0.874761	5.24E-68	-1.69E-46
MS.gene056322	MS.gene056323	0.891296	4.52E-74	-1.69E-46
MS.gene056322	MS.gene46285	0.905786	2.97E-80	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene056322	MS.gene00459	PPI
MS.gene056322	MS.gene02711	PPI
MS.gene044075	MS.gene056322	PPI
MS.gene056322	MS.gene002921	PPI
MS.gene056322	MS.gene09999	PPI
MS.gene056322	MS.gene01879	PPI
MS.gene056322	MS.gene00848	PPI
MS.gene20332	MS.gene056322	PPI
MS.gene056322	MS.gene67184	PPI
MS.gene056322	MS.gene60527	PPI
MS.gene056322	MS.gene005801	PPI
MS.gene056322	MS.gene047961	PPI
MS.gene24439	MS.gene056322	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056322.t1	MTR_4g065080	95.000	260	12	1	1	260	141	399	0.0	506
MS.gene056322.t1	MTR_2g098240	61.887	265	58	6	1	261	135	360	3.65e-102	303
MS.gene056322.t1	MTR_8g011780	47.280	239	90	6	1	229	149	361	9.09e-65	208
MS.gene056322.t1	MTR_7g053200	41.434	251	132	4	1	251	139	374	1.96e-60	197
MS.gene056322.t1	MTR_4g061610	42.241	232	82	5	2	230	763	945	1.74e-47	169
MS.gene056322.t1	MTR_8g070530	39.565	230	91	5	1	227	718	902	6.79e-47	167
MS.gene056322.t1	MTR_7g104180	38.272	243	132	5	1	233	613	847	9.34e-47	167
MS.gene056322.t1	MTR_3g010260	40.486	247	123	7	1	233	536	772	9.43e-47	166
MS.gene056322.t1	MTR_8g446990	39.130	253	116	6	1	233	253	487	1.08e-46	163
MS.gene056322.t1	MTR_2g095980	44.206	233	89	5	1	230	747	941	4.44e-46	165
MS.gene056322.t1	MTR_2g095980	44.206	233	89	5	1	230	747	941	6.94e-46	164
MS.gene056322.t1	MTR_1g060790	37.500	264	139	7	1	250	591	842	1.13e-45	164
MS.gene056322.t1	MTR_4g113790	39.608	255	116	7	1	230	217	458	1.16e-45	160
MS.gene056322.t1	MTR_2g085065	38.865	229	121	5	1	219	213	432	2.26e-45	159
MS.gene056322.t1	MTR_8g089420	40.928	237	115	7	1	229	204	423	1.62e-44	156
MS.gene056322.t1	MTR_2g027240	40.562	249	116	7	1	229	325	561	4.92e-44	157
MS.gene056322.t1	MTR_2g062850	38.662	269	104	9	1	240	290	526	1.85e-43	155
MS.gene056322.t1	MTR_6g004720	38.017	242	125	6	1	229	372	601	2.04e-42	153
MS.gene056322.t1	MTR_2g018990	38.136	236	123	5	1	230	208	426	4.46e-42	150
MS.gene056322.t1	MTR_8g006870	39.224	232	118	5	1	219	491	712	7.61e-42	152
MS.gene056322.t1	MTR_7g081665	38.889	234	134	4	1	229	485	714	1.67e-41	152
MS.gene056322.t1	MTR_7g033640	36.255	251	124	7	1	229	353	589	5.19e-41	149
MS.gene056322.t1	MTR_2g069990	39.744	234	102	8	1	226	215	417	5.46e-41	147
MS.gene056322.t1	MTR_2g095980	56.911	123	49	2	111	230	565	686	7.21e-37	138
MS.gene056322.t1	MTR_6g470940	32.906	234	110	7	1	230	994	1184	7.48e-33	127
MS.gene056322.t1	MTR_5g069000	31.086	267	130	9	2	258	881	1103	1.16e-29	118
MS.gene056322.t1	MTR_4g035835	30.712	267	131	9	2	258	877	1099	2.21e-29	117
MS.gene056322.t1	MTR_1g019340	33.778	225	110	8	4	222	228	419	1.60e-27	111
MS.gene056322.t1	MTR_1g019340	33.778	225	110	8	4	222	228	419	1.72e-27	110
MS.gene056322.t1	MTR_1g069735	32.627	236	119	8	4	234	240	440	4.13e-26	106
MS.gene056322.t1	MTR_1g069735	32.627	236	119	8	4	234	240	440	4.41e-26	106
MS.gene056322.t1	MTR_3g104700	32.068	237	105	7	4	222	214	412	5.03e-26	106
MS.gene056322.t1	MTR_1g069735	32.627	236	119	8	4	234	240	440	5.98e-26	106
MS.gene056322.t1	MTR_7g097150	32.353	238	117	9	4	234	240	440	2.56e-25	105
MS.gene056322.t1	MTR_2g040150	34.211	228	110	9	4	221	233	430	4.66e-25	103
MS.gene056322.t1	MTR_1g075880	31.356	236	123	7	4	234	237	438	3.73e-24	101
MS.gene056322.t1	MTR_3g104700	30.315	254	104	8	4	222	214	429	3.44e-23	99.0
MS.gene056322.t1	MTR_1g042700	31.064	235	125	7	4	234	235	436	1.50e-22	97.1
MS.gene056322.t1	MTR_4g107280	39.370	127	70	4	109	230	209	333	1.60e-22	95.9
MS.gene056322.t1	MTR_7g073520	40.769	130	70	4	105	229	296	423	2.24e-22	96.3
MS.gene056322.t1	MTR_4g107280	39.259	135	72	5	109	238	248	377	2.79e-22	95.5
MS.gene056322.t1	MTR_1g442790	29.555	247	114	9	4	234	230	432	3.40e-22	95.9
MS.gene056322.t1	MTR_1g442790	29.555	247	114	9	4	234	230	432	3.84e-22	95.9
MS.gene056322.t1	MTR_6g087000	33.784	148	80	6	109	241	911	1055	8.38e-18	83.6
MS.gene056322.t1	MTR_3g088715	39.450	109	55	3	109	212	24	126	1.98e-17	77.0
MS.gene056322.t1	MTR_1g090917	28.740	254	114	7	1	252	43	231	2.15e-16	78.2
MS.gene056322.t1	MTR_1g090917	28.959	221	93	5	1	219	43	201	2.55e-16	78.2
MS.gene056322.t1	MTR_1g090917	28.959	221	93	5	1	219	168	326	8.34e-16	77.0
MS.gene056322.t1	MTR_1g090917	28.959	221	93	5	1	219	168	326	9.93e-16	77.0
MS.gene056322.t1	MTR_8g035890	37.719	114	60	3	104	212	161	268	4.25e-15	73.6
MS.gene056322.t1	MTR_7g116390	38.776	98	54	3	105	196	166	263	2.80e-14	71.6
MS.gene056322.t1	MTR_8g040300	36.111	108	62	2	104	208	155	258	9.72e-14	69.7
MS.gene056322.t1	MTR_3g110405	36.667	90	54	1	110	196	188	277	3.39e-13	68.6
MS.gene056322.t1	MTR_1g080020	34.021	97	59	1	105	196	157	253	7.40e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056322.t1	AT1G51170	60.000	270	78	6	1	266	147	390	1.21e-105	312
MS.gene056322.t1	AT3G20830	60.886	271	79	7	1	269	146	391	5.54e-103	306
MS.gene056322.t1	AT4G13000	44.715	246	102	4	2	244	141	355	6.00e-64	205
MS.gene056322.t1	AT3G25250	46.414	237	101	4	2	238	144	354	9.08e-64	206
MS.gene056322.t1	AT5G03640	41.633	245	126	7	1	233	660	899	1.57e-50	178
MS.gene056322.t1	AT5G03640	41.633	245	126	7	1	233	660	899	1.57e-50	178
MS.gene056322.t1	AT5G58140	41.702	235	79	5	1	227	696	880	9.04e-49	173
MS.gene056322.t1	AT5G58140	41.702	235	79	5	1	227	696	880	9.04e-49	173
MS.gene056322.t1	AT5G58140	41.702	235	79	5	1	227	696	880	9.04e-49	173
MS.gene056322.t1	AT5G58140	41.702	235	79	5	1	227	696	880	9.04e-49	173
MS.gene056322.t1	AT5G58140	41.702	235	79	5	1	227	696	880	9.04e-49	173
MS.gene056322.t1	AT5G58140	42.358	229	74	5	1	221	696	874	1.60e-48	172
MS.gene056322.t1	AT3G44610	41.558	231	116	4	1	219	198	421	3.66e-48	166
MS.gene056322.t1	AT2G26700	38.163	283	113	7	1	229	208	482	4.49e-48	167
MS.gene056322.t1	AT3G12690	38.889	270	137	11	1	261	304	554	9.24e-48	167
MS.gene056322.t1	AT3G12690	38.889	270	137	11	1	261	304	554	9.24e-48	167
MS.gene056322.t1	AT3G12690	38.889	270	137	11	1	261	304	554	9.24e-48	167
MS.gene056322.t1	AT2G36350	39.326	267	129	8	1	246	678	932	9.75e-48	170
MS.gene056322.t1	AT3G52890	40.000	255	128	7	1	237	657	904	1.03e-47	170
MS.gene056322.t1	AT3G52890	40.000	255	128	7	1	237	657	904	1.03e-47	170
MS.gene056322.t1	AT3G52890	40.000	255	128	7	1	237	657	904	1.03e-47	170
MS.gene056322.t1	AT3G52890	40.000	255	128	7	1	237	657	904	1.03e-47	170
MS.gene056322.t1	AT5G40030	40.316	253	110	8	1	233	233	464	7.57e-47	163
MS.gene056322.t1	AT1G79250	39.286	252	119	7	1	233	265	501	9.63e-46	161
MS.gene056322.t1	AT1G79250	39.286	252	119	7	1	233	265	501	9.63e-46	161
MS.gene056322.t1	AT1G79250	39.286	252	119	7	1	233	265	501	9.63e-46	161
MS.gene056322.t1	AT1G79250	39.286	252	119	7	1	233	265	501	9.63e-46	161
MS.gene056322.t1	AT5G55910	38.776	245	139	5	1	236	228	470	1.56e-44	157
MS.gene056322.t1	AT5G55910	38.776	245	139	5	1	236	228	470	1.56e-44	157
MS.gene056322.t1	AT2G44830	39.918	243	130	5	1	233	482	718	2.14e-44	160
MS.gene056322.t1	AT2G44830	39.918	243	130	5	1	233	482	718	2.14e-44	160
MS.gene056322.t1	AT2G44830	39.918	243	130	5	1	233	482	718	2.14e-44	160
MS.gene056322.t1	AT5G47750	38.281	256	132	7	1	240	310	555	2.18e-44	158
MS.gene056322.t1	AT1G16440	37.657	239	128	5	1	233	234	457	3.20e-43	154
MS.gene056322.t1	AT1G53700	40.870	230	104	8	1	226	213	414	1.45e-42	151
MS.gene056322.t1	AT4G26610	37.200	250	126	8	1	233	242	477	2.10e-42	151
MS.gene056322.t1	AT3G27580	36.882	263	149	6	1	252	301	557	3.15e-42	152
MS.gene056322.t1	AT3G27580	36.882	263	149	6	1	252	301	557	3.15e-42	152
MS.gene056322.t1	AT2G34650	38.397	237	116	8	1	230	199	412	2.27e-40	145
MS.gene056322.t1	AT3G45780	57.025	121	48	2	111	228	849	968	2.06e-39	146
MS.gene056322.t1	AT3G45780	57.025	121	48	2	111	228	849	968	2.06e-39	146
MS.gene056322.t1	AT3G14370	52.500	120	52	3	111	226	292	410	7.38e-35	130
MS.gene056322.t1	AT3G17850	33.191	235	106	7	2	230	1000	1189	1.91e-31	123
MS.gene056322.t1	AT5G62310	29.348	276	139	8	1	265	871	1101	6.78e-28	113
MS.gene056322.t1	AT3G08720	45.802	131	63	5	105	230	288	415	5.28e-27	109
MS.gene056322.t1	AT3G08720	45.802	131	63	5	105	230	288	415	5.28e-27	109
MS.gene056322.t1	AT3G08720	45.802	131	63	5	105	230	288	415	5.28e-27	109
MS.gene056322.t1	AT3G08720	45.802	131	63	5	105	230	288	415	5.28e-27	109
MS.gene056322.t1	AT1G45160	32.189	233	107	7	1	229	787	972	6.53e-27	110
MS.gene056322.t1	AT1G45160	32.189	233	107	7	1	229	787	972	7.86e-27	109
MS.gene056322.t1	AT3G08730	45.385	130	63	5	105	229	282	408	1.92e-26	107
MS.gene056322.t1	AT3G08730	45.385	130	63	5	105	229	282	408	1.92e-26	107
MS.gene056322.t1	AT1G30640	30.864	243	130	7	4	239	147	358	4.61e-25	103
MS.gene056322.t1	AT1G30640	30.864	243	130	7	4	239	240	451	1.07e-24	103
MS.gene056322.t1	AT1G30640	30.864	243	130	7	4	239	240	451	1.07e-24	103
MS.gene056322.t1	AT5G09890	33.482	224	114	8	4	222	222	415	1.86e-24	102
MS.gene056322.t1	AT1G48490	40.650	123	63	4	113	229	1011	1129	2.04e-24	102
MS.gene056322.t1	AT5G09890	33.482	224	114	8	4	222	222	415	2.09e-24	102
MS.gene056322.t1	AT1G48490	40.650	123	63	4	113	229	1010	1128	2.30e-24	102
MS.gene056322.t1	AT1G48490	40.650	123	63	4	113	229	1010	1128	2.30e-24	102
MS.gene056322.t1	AT1G48490	40.650	123	63	4	113	229	1010	1128	2.30e-24	102
MS.gene056322.t1	AT4G33080	31.897	232	113	9	4	222	214	413	4.34e-24	101
MS.gene056322.t1	AT4G33080	31.897	232	113	9	4	222	214	413	4.38e-24	101
MS.gene056322.t1	AT3G23310	30.290	241	121	9	4	234	240	443	2.43e-23	99.4
MS.gene056322.t1	AT5G04510	29.004	231	100	5	1	229	161	329	2.57e-23	98.6
MS.gene056322.t1	AT3G10540	29.437	231	99	5	1	229	162	330	4.06e-23	98.6
MS.gene056322.t1	AT5G04510	29.004	231	100	5	1	229	161	329	4.27e-23	98.6
MS.gene056322.t1	AT4G14350	29.167	240	123	8	4	234	239	440	5.66e-23	98.2
MS.gene056322.t1	AT4G14350	29.167	240	123	8	4	234	239	440	5.66e-23	98.2
MS.gene056322.t1	AT4G14350	29.167	240	123	8	4	234	239	440	5.66e-23	98.2
MS.gene056322.t1	AT4G14350	29.167	240	123	8	4	234	239	440	5.89e-23	98.2
MS.gene056322.t1	AT2G19400	31.739	230	117	8	4	222	225	425	8.51e-23	97.8
MS.gene056322.t1	AT2G20050	40.000	125	65	4	101	220	925	1044	3.57e-21	93.6
MS.gene056322.t1	AT2G20050	40.000	125	65	4	101	220	928	1047	3.57e-21	93.6
MS.gene056322.t1	AT2G20470	30.252	238	121	9	4	234	244	443	6.50e-21	92.4
MS.gene056322.t1	AT2G20470	30.252	238	121	9	4	234	244	443	6.66e-21	92.4
MS.gene056322.t1	AT2G20470	30.252	238	121	9	4	234	244	443	6.66e-21	92.4
MS.gene056322.t1	AT1G03920	28.750	240	123	7	4	234	144	344	2.11e-20	90.5
MS.gene056322.t1	AT1G03920	28.750	240	123	7	4	234	257	457	3.15e-20	90.5
MS.gene056322.t1	AT1G03920	28.750	240	123	7	4	234	257	457	3.15e-20	90.5
MS.gene056322.t1	AT2G45490	40.000	100	57	1	104	200	167	266	5.63e-17	79.3
MS.gene056322.t1	AT5G04510	27.000	200	83	4	1	199	161	298	1.03e-15	75.9
MS.gene056322.t1	AT2G25880	37.363	91	54	1	110	197	144	234	3.53e-13	68.2
MS.gene056322.t1	AT2G25880	37.363	91	54	1	110	197	176	266	3.82e-13	68.6
MS.gene056322.t1	AT2G25880	37.363	91	54	1	110	197	189	279	3.82e-13	68.6
MS.gene056322.t1	AT2G45490	43.750	64	36	0	104	167	167	230	4.15e-13	67.8
MS.gene056322.t1	AT2G25880	37.363	91	54	1	110	197	237	327	6.84e-13	68.2
MS.gene056322.t1	AT3G04530	36.170	94	55	1	108	196	165	258	4.38e-12	65.1
MS.gene056322.t1	AT1G08650	38.298	94	53	2	108	196	169	262	7.16e-12	64.7
MS.gene056322.t1	AT1G08650	38.298	94	53	2	108	196	169	262	7.50e-12	64.7
MS.gene056322.t1	AT4G32830	35.556	90	55	1	110	196	182	271	7.79e-12	64.7
MS.gene056322.t1	AT4G18700	35.238	105	58	4	112	212	183	281	2.94e-11	63.5
MS.gene056322.t1	AT4G04720	33.913	115	64	4	105	213	232	340	8.61e-11	62.4
MS.gene056322.t1	AT4G04720	33.913	115	64	4	105	213	232	340	8.61e-11	62.4

Find 66 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTACATACGAAATGCTTTA+TGG	0.124822	4.4:-40570830	MS.gene056322:CDS
TTATGGTACAACACCGTTTA+AGG	0.147798	4.4:-40570813	MS.gene056322:CDS
GACTGGTGGTGTGGGTATTA+AGG	0.276142	4.4:-40570516	MS.gene056322:CDS
GAATAACCTAATCTCTTCTT+AGG	0.330840	4.4:+40570683	None:intergenic
GATTCTGGAAACTTTGAATC+TGG	0.336949	4.4:+40571112	None:intergenic
GATTCGGCCGCCGTTTCTTC+CGG	0.357637	4.4:-40570591	MS.gene056322:CDS
TTCCGGTCACGTCACGTTAA+CGG	0.379675	4.4:-40571206	MS.gene056322:CDS
GCCGTTGATTGGTGGGCGCT+AGG	0.384672	4.4:-40570857	MS.gene056322:CDS
TTCCTATTCTTCCCCTTAAA+CGG	0.397634	4.4:+40570800	None:intergenic
TATGGTACAACACCGTTTAA+GGG	0.404386	4.4:-40570812	MS.gene056322:CDS
CACCGTTTAAGGGGAAGAAT+AGG	0.424851	4.4:-40570802	MS.gene056322:CDS
ATATATTGACGGAGGTGATT+CGG	0.426007	4.4:-40570607	MS.gene056322:CDS
TCGTCGGAGAAGTTATCAAA+TGG	0.438085	4.4:-40570539	MS.gene056322:CDS
AAGGATCCTAAGAAGAGATT+AGG	0.449695	4.4:-40570689	MS.gene056322:CDS
CCGGAGGTTGTTCGCGGCGA+TGG	0.450150	4.4:-40570890	MS.gene056322:CDS
GACACGAGTTTGCCGTTGAT+TGG	0.450162	4.4:-40570868	MS.gene056322:CDS
GGATTTGATAGAGAAATTGT+TGG	0.456111	4.4:-40570714	MS.gene056322:CDS
CGAGATACGACGATGATGCC+GGG	0.457242	4.4:-40570568	MS.gene056322:CDS
AATGTTCTCATACAACATTC+CGG	0.459976	4.4:-40571223	MS.gene056322:CDS
ATCGTCGAAATTTCTCTCGT+TGG	0.463991	4.4:-40571078	MS.gene056322:CDS
ATCCGTTAACGTGACGTGAC+CGG	0.467839	4.4:+40571204	None:intergenic
CGAGTTTGCCGTTGATTGGT+GGG	0.475316	4.4:-40570864	MS.gene056322:CDS
GGAAACTTTGAATCTGGTAA+AGG	0.475851	4.4:+40571118	None:intergenic
TCGTATCTCGCCGGAAGAAA+CGG	0.477301	4.4:+40570581	None:intergenic
GAAGATTCAGTTGGAAGAAG+AGG	0.480968	4.4:+40571052	None:intergenic
GCGAGATACGACGATGATGC+CGG	0.490002	4.4:-40570569	MS.gene056322:CDS
CCATCGCCGCGAACAACCTC+CGG	0.491113	4.4:+40570890	None:intergenic
GACGGCGACGGCGGCAATGA+CGG	0.491454	4.4:+40570470	None:intergenic
CCGTTGATTGGTGGGCGCTA+GGG	0.492424	4.4:-40570856	MS.gene056322:CDS
CGTCGGAGAAGTTATCAAAT+GGG	0.494423	4.4:-40570538	MS.gene056322:CDS
ACGAGTTTGCCGTTGATTGG+TGG	0.494517	4.4:-40570865	MS.gene056322:CDS
TAAGTCGGCACGAGTTAGTC+CGG	0.501622	4.4:-40570987	MS.gene056322:CDS
TGATTATGAGAAGATTCAGT+TGG	0.504069	4.4:+40571043	None:intergenic
GATAGAGAAATTGTTGGAGA+AGG	0.514447	4.4:-40570708	MS.gene056322:CDS
TGAGTTTCTCAAACGGCGAA+CGG	0.514898	4.4:-40570949	MS.gene056322:CDS
GTCTAACTCGTTCGTCGGAA+CGG	0.541279	4.4:-40570927	MS.gene056322:CDS
GTTTCACCGGAGGTTGTTCG+CGG	0.542329	4.4:-40570896	MS.gene056322:CDS
TCAAATGGGACTGGTGGTGT+GGG	0.549284	4.4:-40570524	MS.gene056322:CDS
CGAAAATTGAGTTTCTCAAA+CGG	0.549771	4.4:-40570956	MS.gene056322:CDS
ATCAAATGGGACTGGTGGTG+TGG	0.553672	4.4:-40570525	MS.gene056322:CDS
TAGGGTTAAGTTTACGTGAG+AGG	0.555263	4.4:+40571176	None:intergenic
GAATCTGGTAAAGGTGGTGG+TGG	0.562043	4.4:+40571127	None:intergenic
GAACGGTCTAACTCGTTCGT+CGG	0.570467	4.4:-40570932	MS.gene056322:CDS
GAACTTGAAGAAGGCTAAGT+CGG	0.571455	4.4:-40571002	MS.gene056322:CDS
AACGGAGGAATATGTTTCAC+CGG	0.576639	4.4:-40570909	MS.gene056322:CDS
TGTACGTTGGGATATATTGA+CGG	0.584593	4.4:-40570618	MS.gene056322:CDS
CCCTAGCGCCCACCAATCAA+CGG	0.593746	4.4:+40570856	None:intergenic
AAATGTGCTGATGAAGTCGC+CGG	0.594698	4.4:-40570765	MS.gene056322:CDS
ATGGTACAACACCGTTTAAG+GGG	0.596720	4.4:-40570811	MS.gene056322:CDS
ATGAAGTCGCCGGAGTTCGT+CGG	0.601089	4.4:-40570755	MS.gene056322:CDS
CTCAACTTACAAGACGGCGA+CGG	0.604669	4.4:+40570458	None:intergenic
AACTTACAAGACGGCGACGG+CGG	0.606977	4.4:+40570461	None:intergenic
GAGAAGTTATCAAATGGGAC+TGG	0.607235	4.4:-40570533	MS.gene056322:CDS
AAGTTATCAAATGGGACTGG+TGG	0.616181	4.4:-40570530	MS.gene056322:CDS
GTCACGGTAAACTATGCCCA+TGG	0.618817	4.4:+40571258	None:intergenic
GCATCATCGTCGTATCTCGC+CGG	0.620999	4.4:+40570572	None:intergenic
TGGTGGTGGTGTTGCAACAA+CGG	0.624705	4.4:+40571141	None:intergenic
TAACTCGTTCGTCGGAACGG+AGG	0.625008	4.4:-40570924	MS.gene056322:CDS
CTTCTCCGACGAAAATTCCC+CGG	0.626589	4.4:+40570550	None:intergenic
TTTGAATCTGGTAAAGGTGG+TGG	0.639756	4.4:+40571124	None:intergenic
AACTTTGAATCTGGTAAAGG+TGG	0.650483	4.4:+40571121	None:intergenic
GGAGGAATATGTTTCACCGG+AGG	0.668658	4.4:-40570906	MS.gene056322:CDS
GAGATACGACGATGATGCCG+GGG	0.680841	4.4:-40570567	MS.gene056322:CDS
AAGAGATTAGGTTATTCACG+TGG	0.689273	4.4:-40570677	MS.gene056322:CDS
TATCTCGCCGGAAGAAACGG+CGG	0.693953	4.4:+40570584	None:intergenic
ACGTTGGGATATATTGACGG+AGG	0.766330	4.4:-40570615	MS.gene056322:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATGTTCTCATACAACATTC+CGG	-	chr4.4:40570501-40570520	MS.gene056322:CDS	30.0%
	CGAAAATTGAGTTTCTCAAA+CGG	-	chr4.4:40570768-40570787	MS.gene056322:CDS	30.0%
	CTTACATACGAAATGCTTTA+TGG	-	chr4.4:40570894-40570913	MS.gene056322:CDS	30.0%
	GAATAACCTAATCTCTTCTT+AGG	+	chr4.4:40571044-40571063	None:intergenic	30.0%
	GGATTTGATAGAGAAATTGT+TGG	-	chr4.4:40571010-40571029	MS.gene056322:CDS	30.0%
	TGATTATGAGAAGATTCAGT+TGG	+	chr4.4:40570684-40570703	None:intergenic	30.0%
!	TGTTGTATGAGAACATTTTC+TGG	+	chr4.4:40570498-40570517	None:intergenic	30.0%
!	TTGAGAAACTCAATTTTCGT+CGG	+	chr4.4:40570767-40570786	None:intergenic	30.0%
!!	AAAGAGCATGCATTTTTCAA+AGG	-	chr4.4:40571083-40571102	MS.gene056322:CDS	30.0%
!!	ATTTTTCAAAGGTGTACGTT+GGG	-	chr4.4:40571094-40571113	MS.gene056322:CDS	30.0%
!!!	ACAACGGTTTTAGAAGTTTT+AGG	+	chr4.4:40570570-40570589	None:intergenic	30.0%
!!!	CAACGGTTTTAGAAGTTTTA+GGG	+	chr4.4:40570569-40570588	None:intergenic	30.0%
!!!	TGAGAACATTTTCTGGTTTT+AGG	+	chr4.4:40570491-40570510	None:intergenic	30.0%
	AACTTTGAATCTGGTAAAGG+TGG	+	chr4.4:40570606-40570625	None:intergenic	35.0%
	AAGAGATTAGGTTATTCACG+TGG	-	chr4.4:40571047-40571066	MS.gene056322:CDS	35.0%
	AAGGATCCTAAGAAGAGATT+AGG	-	chr4.4:40571035-40571054	MS.gene056322:CDS	35.0%
	ATATATTGACGGAGGTGATT+CGG	-	chr4.4:40571117-40571136	MS.gene056322:CDS	35.0%
	GATAGAGAAATTGTTGGAGA+AGG	-	chr4.4:40571016-40571035	MS.gene056322:CDS	35.0%
	GGAAACTTTGAATCTGGTAA+AGG	+	chr4.4:40570609-40570628	None:intergenic	35.0%
	TATGGTACAACACCGTTTAA+GGG	-	chr4.4:40570912-40570931	MS.gene056322:CDS	35.0%
	TGTACGTTGGGATATATTGA+CGG	-	chr4.4:40571106-40571125	MS.gene056322:CDS	35.0%
	TTATGGTACAACACCGTTTA+AGG	-	chr4.4:40570911-40570930	MS.gene056322:CDS	35.0%
	TTCCTATTCTTCCCCTTAAA+CGG	+	chr4.4:40570927-40570946	None:intergenic	35.0%
!	GATTCTGGAAACTTTGAATC+TGG	+	chr4.4:40570615-40570634	None:intergenic	35.0%
!!	CATTTTTCAAAGGTGTACGT+TGG	-	chr4.4:40571093-40571112	MS.gene056322:CDS	35.0%
!!	GCATGTTTTGAACTTGAAGA+AGG	-	chr4.4:40570713-40570732	MS.gene056322:CDS	35.0%
!!!	CATTTTCTGGTTTTAGGTCA+CGG	+	chr4.4:40570485-40570504	None:intergenic	35.0%
	AACGGAGGAATATGTTTCAC+CGG	-	chr4.4:40570815-40570834	MS.gene056322:CDS	40.0%
	AAGTTATCAAATGGGACTGG+TGG	-	chr4.4:40571194-40571213	MS.gene056322:CDS	40.0%
	ATCGTCGAAATTTCTCTCGT+TGG	-	chr4.4:40570646-40570665	MS.gene056322:CDS	40.0%
	ATGGTACAACACCGTTTAAG+GGG	-	chr4.4:40570913-40570932	MS.gene056322:CDS	40.0%
	CGTCGGAGAAGTTATCAAAT+GGG	-	chr4.4:40571186-40571205	MS.gene056322:CDS	40.0%
	GAACTTGAAGAAGGCTAAGT+CGG	-	chr4.4:40570722-40570741	MS.gene056322:CDS	40.0%
	GAAGATTCAGTTGGAAGAAG+AGG	+	chr4.4:40570675-40570694	None:intergenic	40.0%
	GAGAAGTTATCAAATGGGAC+TGG	-	chr4.4:40571191-40571210	MS.gene056322:CDS	40.0%
	TAGGGTTAAGTTTACGTGAG+AGG	+	chr4.4:40570551-40570570	None:intergenic	40.0%
	TCGTCGGAGAAGTTATCAAA+TGG	-	chr4.4:40571185-40571204	MS.gene056322:CDS	40.0%
	TTTGAATCTGGTAAAGGTGG+TGG	+	chr4.4:40570603-40570622	None:intergenic	40.0%
	ACGTTGGGATATATTGACGG+AGG	-	chr4.4:40571109-40571128	MS.gene056322:CDS	45.0%
	CACCGTTTAAGGGGAAGAAT+AGG	-	chr4.4:40570922-40570941	MS.gene056322:CDS	45.0%
	CGGAAAAAAGACGGAGTTGA+CGG	-	chr4.4:40570989-40571008	MS.gene056322:CDS	45.0%
	GGAGTTCGTCGGAAAAAAGA+CGG	-	chr4.4:40570980-40570999	MS.gene056322:CDS	45.0%
	TGAGTTTCTCAAACGGCGAA+CGG	-	chr4.4:40570775-40570794	MS.gene056322:CDS	45.0%
!	AAATGTGCTGATGAAGTCGC+CGG	-	chr4.4:40570959-40570978	MS.gene056322:CDS	45.0%
!	CGATGTTTTCGCGAAGATTC+TGG	+	chr4.4:40570630-40570649	None:intergenic	45.0%
!!!	GTCTTTTTTCCGACGAACTC+CGG	+	chr4.4:40570981-40571000	None:intergenic	45.0%
	ATCCGTTAACGTGACGTGAC+CGG	+	chr4.4:40570523-40570542	None:intergenic	50.0%
	CTTCTCCGACGAAAATTCCC+CGG	+	chr4.4:40571177-40571196	None:intergenic	50.0%
	GAACGGTCTAACTCGTTCGT+CGG	-	chr4.4:40570792-40570811	MS.gene056322:CDS	50.0%
	GACACGAGTTTGCCGTTGAT+TGG	-	chr4.4:40570856-40570875	MS.gene056322:CDS	50.0%
	GGAGGAATATGTTTCACCGG+AGG	-	chr4.4:40570818-40570837	MS.gene056322:CDS	50.0%
	GTCTAACTCGTTCGTCGGAA+CGG	-	chr4.4:40570797-40570816	MS.gene056322:CDS	50.0%
	TAAGTCGGCACGAGTTAGTC+CGG	-	chr4.4:40570737-40570756	MS.gene056322:CDS	50.0%
	TCGTATCTCGCCGGAAGAAA+CGG	+	chr4.4:40571146-40571165	None:intergenic	50.0%
	TTCCGGTCACGTCACGTTAA+CGG	-	chr4.4:40570518-40570537	MS.gene056322:CDS	50.0%
!	ACGAGTTTGCCGTTGATTGG+TGG	-	chr4.4:40570859-40570878	MS.gene056322:CDS	50.0%
!	ATCAAATGGGACTGGTGGTG+TGG	-	chr4.4:40571199-40571218	MS.gene056322:CDS	50.0%
!	CGAGTTTGCCGTTGATTGGT+GGG	-	chr4.4:40570860-40570879	MS.gene056322:CDS	50.0%
!	GAATCTGGTAAAGGTGGTGG+TGG	+	chr4.4:40570600-40570619	None:intergenic	50.0%
!	GACTGGTGGTGTGGGTATTA+AGG	-	chr4.4:40571208-40571227	MS.gene056322:CDS	50.0%
!	TCAAATGGGACTGGTGGTGT+GGG	-	chr4.4:40571200-40571219	MS.gene056322:CDS	50.0%
!	TGATGCCGGGGAATTTTCGT+CGG	-	chr4.4:40571169-40571188	MS.gene056322:CDS	50.0%
!!	TGGTGGTGGTGTTGCAACAA+CGG	+	chr4.4:40570586-40570605	None:intergenic	50.0%
!!!	CTCAATTTTCGTCGGCTCAC+CGG	+	chr4.4:40570759-40570778	None:intergenic	50.0%
	ATGAAGTCGCCGGAGTTCGT+CGG	-	chr4.4:40570969-40570988	MS.gene056322:CDS	55.0%
	CGAGATACGACGATGATGCC+GGG	-	chr4.4:40571156-40571175	MS.gene056322:CDS	55.0%
	GAGATACGACGATGATGCCG+GGG	-	chr4.4:40571157-40571176	MS.gene056322:CDS	55.0%
	GCATCATCGTCGTATCTCGC+CGG	+	chr4.4:40571155-40571174	None:intergenic	55.0%
	GCGAGATACGACGATGATGC+CGG	-	chr4.4:40571155-40571174	MS.gene056322:CDS	55.0%
	GTTTCACCGGAGGTTGTTCG+CGG	-	chr4.4:40570828-40570847	MS.gene056322:CDS	55.0%
	TAACTCGTTCGTCGGAACGG+AGG	-	chr4.4:40570800-40570819	MS.gene056322:CDS	55.0%
	TATCTCGCCGGAAGAAACGG+CGG	+	chr4.4:40571143-40571162	None:intergenic	55.0%
	CCCTAGCGCCCACCAATCAA+CGG	+	chr4.4:40570871-40570890	None:intergenic	60.0%
	GATTCGGCCGCCGTTTCTTC+CGG	-	chr4.4:40571133-40571152	MS.gene056322:CDS	60.0%
!	CCGTTGATTGGTGGGCGCTA+GGG	-	chr4.4:40570868-40570887	MS.gene056322:CDS	60.0%
	CCATCGCCGCGAACAACCTC+CGG	+	chr4.4:40570837-40570856	None:intergenic	65.0%
!	GCCGTTGATTGGTGGGCGCT+AGG	-	chr4.4:40570867-40570886	MS.gene056322:CDS	65.0%
	CCGGAGGTTGTTCGCGGCGA+TGG	-	chr4.4:40570834-40570853	MS.gene056322:CDS	70.0%
	GACGGCGACGGCGGCAATGA+CGG	+	chr4.4:40571257-40571276	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	40570468	40571278	40570468	ID=MS.gene056322
chr4.4	mRNA	40570468	40571278	40570468	ID=MS.gene056322.t1;Parent=MS.gene056322
chr4.4	exon	40570468	40571278	40570468	ID=MS.gene056322.t1.exon1;Parent=MS.gene056322.t1
chr4.4	CDS	40570468	40571278	40570468	ID=cds.MS.gene056322.t1;Parent=MS.gene056322.t1

Gene Sequence

>MS.gene056322

ATGGGCATAGTTTACCGTGACCTAAAACCAGAAAATGTTCTCATACAACATTCCGGTCACGTCACGTTAACGGATTTCGACCTCTCACGTAAACTTAACCCTAAAACTTCTAAAACCGTTGTTGCAACACCACCACCACCTTTACCAGATTCAAAGTTTCCAGAATCTTCGCGAAAACATCGTCGAAATTTCTCTCGTTGGATACCTCTTCTTCCAACTGAATCTTCTCATAATCATAATAAGATGCATGTTTTGAACTTGAAGAAGGCTAAGTCGGCACGAGTTAGTCCGGTGAGCCGACGAAAATTGAGTTTCTCAAACGGCGAACGGTCTAACTCGTTCGTCGGAACGGAGGAATATGTTTCACCGGAGGTTGTTCGCGGCGATGGACACGAGTTTGCCGTTGATTGGTGGGCGCTAGGGATACTTACATACGAAATGCTTTATGGTACAACACCGTTTAAGGGGAAGAATAGGAAAGAAACGTTTAAAAATGTGCTGATGAAGTCGCCGGAGTTCGTCGGAAAAAAGACGGAGTTGACGGATTTGATAGAGAAATTGTTGGAGAAGGATCCTAAGAAGAGATTAGGTTATTCACGTGGAGCTGTTGAGATTAAAGAGCATGCATTTTTCAAAGGTGTACGTTGGGATATATTGACGGAGGTGATTCGGCCGCCGTTTCTTCCGGCGAGATACGACGATGATGCCGGGGAATTTTCGTCGGAGAAGTTATCAAATGGGACTGGTGGTGTGGGTATTAAGGACTATTTTGAAAATTTGAAATCGCCGTCATTGCCGCCGTCGCCGTCTT

Protein sequence

>MS.gene056322.t1

MGIVYRDLKPENVLIQHSGHVTLTDFDLSRKLNPKTSKTVVATPPPPLPDSKFPESSRKHRRNFSRWIPLLPTESSHNHNKMHVLNLKKAKSARVSPVSRRKLSFSNGERSNSFVGTEEYVSPEVVRGDGHEFAVDWWALGILTYEMLYGTTPFKGKNRKETFKNVLMKSPEFVGKKTELTDLIEKLLEKDPKKRLGYSRGAVEIKEHAFFKGVRWDILTEVIRPPFLPARYDDDAGEFSSEKLSNGTGGVGIKDYFENLKSPSLPPSPS