Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056650.t1 | Q9FVL0.1 | 100 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 1.90E-82 | 315.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056650.t1 | Q9FVL0 | 100.0 | 160 | 0 | 0 | 1 | 160 | 1 | 160 | 4.6e-85 | 315.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056650.t1 | I3SPW2 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 4.0e-82 | 313.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056647 | MS.gene056650 | 0.803967 | 2.71E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056650.t1 | MTR_4g068860 | 99.375 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 1.42e-115 | 324 |
| MS.gene056650.t1 | MTR_4g068870 | 83.221 | 149 | 25 | 0 | 9 | 157 | 49 | 197 | 3.93e-87 | 254 |
| MS.gene056650.t1 | MTR_4g068870 | 83.221 | 149 | 25 | 0 | 9 | 157 | 132 | 280 | 5.04e-86 | 254 |
| MS.gene056650.t1 | MTR_4g068870 | 84.404 | 109 | 17 | 0 | 49 | 157 | 1 | 109 | 7.34e-61 | 190 |
| MS.gene056650.t1 | MTR_4g068870 | 80.128 | 156 | 31 | 0 | 2 | 157 | 171 | 326 | 1.33e-85 | 254 |
| MS.gene056650.t1 | MTR_4g068870 | 79.487 | 156 | 29 | 1 | 2 | 157 | 10 | 162 | 9.22e-84 | 250 |
| MS.gene056650.t1 | MTR_4g068870 | 83.221 | 149 | 25 | 0 | 9 | 157 | 185 | 333 | 2.72e-85 | 254 |
| MS.gene056650.t1 | MTR_4g068870 | 79.487 | 156 | 29 | 1 | 2 | 157 | 10 | 162 | 1.15e-83 | 250 |
| MS.gene056650.t1 | MTR_0026s0210 | 81.333 | 150 | 28 | 0 | 8 | 157 | 13 | 162 | 4.71e-85 | 253 |
| MS.gene056650.t1 | MTR_0026s0210 | 81.879 | 149 | 27 | 0 | 9 | 157 | 185 | 333 | 1.63e-84 | 252 |
| MS.gene056650.t1 | MTR_4g068860 | 96.694 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 1.45e-82 | 239 |
| MS.gene056650.t1 | MTR_1g090810 | 48.000 | 150 | 77 | 1 | 8 | 156 | 3 | 152 | 2.06e-38 | 128 |
| MS.gene056650.t1 | MTR_5g041610 | 47.651 | 149 | 74 | 2 | 8 | 156 | 2 | 146 | 8.56e-37 | 124 |
| MS.gene056650.t1 | MTR_5g081030 | 45.638 | 149 | 77 | 2 | 8 | 156 | 2 | 146 | 2.00e-36 | 123 |
| MS.gene056650.t1 | MTR_5g080440 | 45.638 | 149 | 77 | 2 | 8 | 156 | 2 | 146 | 4.29e-36 | 122 |
| MS.gene056650.t1 | MTR_1g011540 | 47.651 | 149 | 73 | 3 | 8 | 156 | 2 | 145 | 3.22e-35 | 120 |
| MS.gene056650.t1 | MTR_5g081000 | 46.309 | 149 | 75 | 3 | 8 | 156 | 2 | 145 | 4.01e-34 | 117 |
| MS.gene056650.t1 | MTR_5g066070 | 45.270 | 148 | 77 | 2 | 9 | 156 | 3 | 146 | 8.36e-34 | 116 |
| MS.gene056650.t1 | MTR_5g080400 | 44.000 | 150 | 80 | 2 | 8 | 157 | 2 | 147 | 4.34e-33 | 115 |
| MS.gene056650.t1 | MTR_1g090820 | 44.295 | 149 | 80 | 1 | 8 | 156 | 2 | 147 | 7.23e-33 | 114 |
| MS.gene056650.t1 | MTR_1g049330 | 41.892 | 148 | 83 | 1 | 9 | 156 | 3 | 147 | 1.04e-30 | 108 |
| MS.gene056650.t1 | MTR_5g080900 | 39.189 | 148 | 71 | 4 | 9 | 156 | 3 | 131 | 6.29e-23 | 88.2 |
| MS.gene056650.t1 | MTR_1g090820 | 46.296 | 108 | 55 | 1 | 49 | 156 | 1 | 105 | 1.33e-22 | 86.7 |
| MS.gene056650.t1 | MTR_5g080400 | 44.954 | 109 | 56 | 2 | 49 | 157 | 1 | 105 | 2.09e-22 | 86.3 |
| MS.gene056650.t1 | MTR_5g066070 | 43.519 | 108 | 57 | 2 | 9 | 116 | 3 | 106 | 6.38e-21 | 82.4 |
| MS.gene056650.t1 | MTR_1g090820 | 41.284 | 109 | 61 | 1 | 8 | 116 | 2 | 107 | 1.22e-19 | 79.3 |
| MS.gene056650.t1 | MTR_7g110180 | 40.833 | 120 | 54 | 5 | 9 | 128 | 3 | 105 | 1.37e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056650.t1 | AT2G16060 | 79.605 | 152 | 31 | 0 | 9 | 160 | 9 | 160 | 1.85e-88 | 255 |
| MS.gene056650.t1 | AT3G10520 | 52.667 | 150 | 71 | 0 | 8 | 157 | 5 | 154 | 6.67e-51 | 160 |
Find 27 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGACAAAGTTTGCACTTT+TGG | 0.188019 | 4.3:+36344698 | MS.gene056650:intron |
| CTACTACACCGTATTTAAAA+TGG | 0.249717 | 4.3:-36344469 | None:intergenic |
| TTCTCTGACCGTAACTTTAC+CGG | 0.314302 | 4.3:-36344420 | None:intergenic |
| GAAAGATTCAAAAGTTCCTT+TGG | 0.329995 | 4.3:+36344064 | MS.gene056650:CDS |
| GCCGTTCAACTGCGGAAATC+CGG | 0.382799 | 4.3:+36344401 | MS.gene056650:CDS |
| AAGAAGAATTCTGCAGAGTT+AGG | 0.397154 | 4.3:+36343878 | MS.gene056650:CDS |
| ATAGCCGGTGACCACATTTC+AGG | 0.433187 | 4.3:-36344741 | None:intergenic |
| CCTTGAGAATATGGGCACTT+TGG | 0.447264 | 4.3:+36343796 | None:intergenic |
| ATGGGCACTTTGGATACAAA+AGG | 0.471419 | 4.3:+36343806 | MS.gene056650:CDS |
| ACCGGATTTCCGCAGTTGAA+CGG | 0.472823 | 4.3:-36344402 | None:intergenic |
| CCCCATGCATTCTTCATAGC+CGG | 0.478259 | 4.3:-36344756 | None:intergenic |
| TGCCATGTCTGTCTTTCTCA+TGG | 0.508395 | 4.3:+36344112 | MS.gene056650:CDS |
| GCGGAAATCCGGTAAAGTTA+CGG | 0.520140 | 4.3:+36344412 | MS.gene056650:CDS |
| CACCGGCTATGAAGAATGCA+TGG | 0.521161 | 4.3:+36344754 | MS.gene056650:CDS |
| AAACTGAAAGAGAATCTAAG+AGG | 0.524334 | 4.3:-36344829 | None:intergenic |
| AAGCTCTTGTGGTGAAGTCA+TGG | 0.525782 | 4.3:+36343846 | MS.gene056650:CDS |
| GGGAGAAGCTTATGATCAGT+TGG | 0.552837 | 4.3:+36344776 | MS.gene056650:CDS |
| ACCGGCTATGAAGAATGCAT+GGG | 0.592597 | 4.3:+36344755 | MS.gene056650:CDS |
| TGAAAGAGAATCTAAGAGGA+AGG | 0.606498 | 4.3:-36344825 | None:intergenic |
| AGTACCTGAAATGTGGTCAC+CGG | 0.611768 | 4.3:+36344737 | MS.gene056650:CDS |
| CATGGCATGAGGCTTGAGCT+TGG | 0.612875 | 4.3:-36344096 | None:intergenic |
| TGAAGAACAAGAAGCTCTTG+TGG | 0.616139 | 4.3:+36343835 | MS.gene056650:CDS |
| AAGAAGCAGTACCTGAAATG+TGG | 0.626355 | 4.3:+36344730 | MS.gene056650:CDS |
| GTGAATCAGCCGTTCAACTG+CGG | 0.649145 | 4.3:+36344393 | MS.gene056650:CDS |
| AGAAAGACAGACATGGCATG+AGG | 0.665594 | 4.3:-36344107 | None:intergenic |
| CCGGCTATGAAGAATGCATG+GGG | 0.704265 | 4.3:+36344756 | MS.gene056650:CDS |
| TACCATGAGAAAGACAGACA+TGG | 0.720993 | 4.3:-36344114 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATTTTTTGATTTTGTAA+AGG | + | chr4.3:36344677-36344696 | MS.gene056650:intron | 10.0% |
| !!! | TTTTTTCTTCTATATTCAAA+TGG | - | chr4.3:36344344-36344363 | None:intergenic | 15.0% |
| !! | ACAATAACAAAATAATTAGC+TGG | - | chr4.3:36344367-36344386 | None:intergenic | 20.0% |
| !! | TTACATTGAAATTTGACATA+AGG | + | chr4.3:36344577-36344596 | MS.gene056650:intron | 20.0% |
| !!! | CTAATTCTGTAACTAAATTT+TGG | + | chr4.3:36344205-36344224 | MS.gene056650:intron | 20.0% |
| !!! | TTTAACATTTTTAACTCAAG+AGG | + | chr4.3:36343938-36343957 | MS.gene056650:intron | 20.0% |
| ! | AGATTATTGTTCAATATGCA+GGG | + | chr4.3:36344613-36344632 | MS.gene056650:intron | 25.0% |
| ! | GAATCAAGCTTGAAAAAATT+AGG | + | chr4.3:36344440-36344459 | MS.gene056650:CDS | 25.0% |
| ! | TAGATTATTGTTCAATATGC+AGG | + | chr4.3:36344612-36344631 | MS.gene056650:intron | 25.0% |
| ! | TTTAGTTACAGAATTAGAGA+CGG | - | chr4.3:36344202-36344221 | None:intergenic | 25.0% |
| CTACTACACCGTATTTAAAA+TGG | - | chr4.3:36344472-36344491 | None:intergenic | 30.0% | |
| GAAAGATTCAAAAGTTCCTT+TGG | + | chr4.3:36344064-36344083 | MS.gene056650:CDS | 30.0% | |
| GATTATTGTTCAATATGCAG+GGG | + | chr4.3:36344614-36344633 | MS.gene056650:intron | 30.0% | |
| TTCTATATTCAAATGGTGAG+TGG | - | chr4.3:36344337-36344356 | None:intergenic | 30.0% | |
| ! | GGTGCTAACCATTTTAAATA+CGG | + | chr4.3:36344461-36344480 | MS.gene056650:CDS | 30.0% |
| ! | TTTTCTTGAAGTAAGTAGCT+AGG | + | chr4.3:36343909-36343928 | MS.gene056650:intron | 30.0% |
| !!! | TTTTTAGTGTCATTTCGCAT+CGG | + | chr4.3:36344278-36344297 | MS.gene056650:intron | 30.0% |
| AAGAAGAATTCTGCAGAGTT+AGG | + | chr4.3:36343878-36343897 | MS.gene056650:CDS | 35.0% | |
| TGACAAAGTCAAAGCTTCAA+TGG | - | chr4.3:36344307-36344326 | None:intergenic | 35.0% | |
| TGGACTGAGTATATAAGAGA+CGG | + | chr4.3:36344225-36344244 | MS.gene056650:intron | 35.0% | |
| !! | GAGAAAAGTTTTTGAGCTGA+TGG | - | chr4.3:36344038-36344057 | None:intergenic | 35.0% |
| !! | TTCAGGTACTGCTTCTTTTA+TGG | - | chr4.3:36344727-36344746 | None:intergenic | 35.0% |
| AAGAAGCAGTACCTGAAATG+TGG | + | chr4.3:36344730-36344749 | MS.gene056650:CDS | 40.0% | |
| ATGGGCACTTTGGATACAAA+AGG | + | chr4.3:36343806-36343825 | MS.gene056650:CDS | 40.0% | |
| TACCATGAGAAAGACAGACA+TGG | - | chr4.3:36344117-36344136 | None:intergenic | 40.0% | |
| TGAAGAACAAGAAGCTCTTG+TGG | + | chr4.3:36343835-36343854 | MS.gene056650:CDS | 40.0% | |
| TTCTCTGACCGTAACTTTAC+CGG | - | chr4.3:36344423-36344442 | None:intergenic | 40.0% | |
| ! | GGTGACAAAGTTTGCACTTT+TGG | + | chr4.3:36344698-36344717 | MS.gene056650:intron | 40.0% |
| ! | TGTAGTAGACGAGCATTTTG+AGG | + | chr4.3:36344484-36344503 | MS.gene056650:CDS | 40.0% |
| AAGCTCTTGTGGTGAAGTCA+TGG | + | chr4.3:36343846-36343865 | MS.gene056650:CDS | 45.0% | |
| ACCGGCTATGAAGAATGCAT+GGG | + | chr4.3:36344755-36344774 | MS.gene056650:CDS | 45.0% | |
| AGAAAGACAGACATGGCATG+AGG | - | chr4.3:36344110-36344129 | None:intergenic | 45.0% | |
| AGTACCTGAAATGTGGTCAC+CGG | + | chr4.3:36344737-36344756 | MS.gene056650:CDS | 45.0% | |
| GCGGAAATCCGGTAAAGTTA+CGG | + | chr4.3:36344412-36344431 | MS.gene056650:CDS | 45.0% | |
| GGGAGAAGCTTATGATCAGT+TGG | + | chr4.3:36344776-36344795 | MS.gene056650:CDS | 45.0% | |
| TGCCATGTCTGTCTTTCTCA+TGG | + | chr4.3:36344112-36344131 | MS.gene056650:CDS | 45.0% | |
| !!! | AGCTTGGTGTTTTGCTCCAA+AGG | - | chr4.3:36344083-36344102 | None:intergenic | 45.0% |
| ACCGGATTTCCGCAGTTGAA+CGG | - | chr4.3:36344405-36344424 | None:intergenic | 50.0% | |
| ATAGCCGGTGACCACATTTC+AGG | - | chr4.3:36344744-36344763 | None:intergenic | 50.0% | |
| CCCCATGCATTCTTCATAGC+CGG | - | chr4.3:36344759-36344778 | None:intergenic | 50.0% | |
| CCGGCTATGAAGAATGCATG+GGG | + | chr4.3:36344756-36344775 | MS.gene056650:CDS | 50.0% | |
| GTGAATCAGCCGTTCAACTG+CGG | + | chr4.3:36344393-36344412 | MS.gene056650:CDS | 50.0% | |
| ! | CACCGGCTATGAAGAATGCA+TGG | + | chr4.3:36344754-36344773 | MS.gene056650:CDS | 50.0% |
| CATGGCATGAGGCTTGAGCT+TGG | - | chr4.3:36344099-36344118 | None:intergenic | 55.0% | |
| GCCGTTCAACTGCGGAAATC+CGG | + | chr4.3:36344401-36344420 | MS.gene056650:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 36343806 | 36344836 | 36343806 | ID=MS.gene056650 |
| chr4.3 | mRNA | 36343806 | 36344836 | 36343806 | ID=MS.gene056650.t1;Parent=MS.gene056650 |
| chr4.3 | exon | 36343806 | 36343918 | 36343806 | ID=MS.gene056650.t1.exon1;Parent=MS.gene056650.t1 |
| chr4.3 | CDS | 36343806 | 36343918 | 36343806 | ID=cds.MS.gene056650.t1;Parent=MS.gene056650.t1 |
| chr4.3 | exon | 36344019 | 36344133 | 36344019 | ID=MS.gene056650.t1.exon2;Parent=MS.gene056650.t1 |
| chr4.3 | CDS | 36344019 | 36344133 | 36344019 | ID=cds.MS.gene056650.t1;Parent=MS.gene056650.t1 |
| chr4.3 | exon | 36344389 | 36344505 | 36344389 | ID=MS.gene056650.t1.exon3;Parent=MS.gene056650.t1 |
| chr4.3 | CDS | 36344389 | 36344505 | 36344389 | ID=cds.MS.gene056650.t1;Parent=MS.gene056650.t1 |
| chr4.3 | exon | 36344699 | 36344836 | 36344699 | ID=MS.gene056650.t1.exon4;Parent=MS.gene056650.t1 |
| chr4.3 | CDS | 36344699 | 36344836 | 36344699 | ID=cds.MS.gene056650.t1;Parent=MS.gene056650.t1 |
| Gene Sequence |
| Protein sequence |
