Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056669.t1 | XP_013456373.1 | 99.2 | 374 | 3 | 0 | 1 | 374 | 1 | 374 | 4.00E-184 | 654.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056669.t1 | Q9SYZ7 | 74.6 | 374 | 88 | 2 | 1 | 373 | 1 | 368 | 4.6e-136 | 485.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056669.t1 | A0A072UL03 | 99.2 | 374 | 3 | 0 | 1 | 374 | 1 | 374 | 2.9e-184 | 654.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056669 | MS.gene69205 | 0.859203 | 4.86E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056669.t1 | MTR_4g069160 | 99.198 | 374 | 3 | 0 | 1 | 374 | 1 | 374 | 0.0 | 761 |
| MS.gene056669.t1 | MTR_1g034480 | 75.936 | 374 | 85 | 3 | 1 | 373 | 1 | 370 | 0.0 | 535 |
| MS.gene056669.t1 | MTR_5g019430 | 57.937 | 378 | 152 | 4 | 1 | 371 | 1 | 378 | 6.94e-153 | 436 |
| MS.gene056669.t1 | MTR_8g078100 | 58.090 | 377 | 152 | 4 | 1 | 373 | 1 | 375 | 6.53e-143 | 410 |
| MS.gene056669.t1 | MTR_3g011140 | 56.164 | 73 | 31 | 1 | 284 | 355 | 1 | 73 | 1.31e-19 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056669.t1 | AT4G34320 | 74.599 | 374 | 88 | 2 | 1 | 373 | 1 | 368 | 0.0 | 528 |
| MS.gene056669.t1 | AT4G34320 | 74.599 | 374 | 88 | 2 | 1 | 373 | 1 | 368 | 0.0 | 528 |
| MS.gene056669.t1 | AT4G34330 | 58.537 | 369 | 132 | 3 | 1 | 369 | 1 | 348 | 2.08e-136 | 393 |
| MS.gene056669.t1 | AT2G18630 | 53.968 | 378 | 168 | 4 | 1 | 372 | 2 | 379 | 4.54e-135 | 391 |
| MS.gene056669.t1 | AT2G18630 | 53.968 | 378 | 168 | 4 | 1 | 372 | 2 | 379 | 6.28e-135 | 390 |
| MS.gene056669.t1 | AT5G66675 | 51.389 | 360 | 164 | 2 | 25 | 373 | 38 | 397 | 3.35e-123 | 362 |
| MS.gene056669.t1 | AT5G66675 | 51.389 | 360 | 164 | 2 | 25 | 373 | 38 | 397 | 3.35e-123 | 362 |
| MS.gene056669.t1 | AT5G66675 | 51.389 | 360 | 164 | 2 | 25 | 373 | 38 | 397 | 3.35e-123 | 362 |
| MS.gene056669.t1 | AT5G66675 | 51.389 | 360 | 164 | 2 | 25 | 373 | 38 | 397 | 3.35e-123 | 362 |
| MS.gene056669.t1 | AT5G66670 | 40.997 | 361 | 203 | 2 | 19 | 369 | 32 | 392 | 3.24e-89 | 275 |
| MS.gene056669.t1 | AT5G66670 | 40.997 | 361 | 203 | 2 | 19 | 369 | 32 | 392 | 3.24e-89 | 275 |
| MS.gene056669.t1 | AT5G66670 | 40.997 | 361 | 203 | 2 | 19 | 369 | 32 | 392 | 3.24e-89 | 275 |
| MS.gene056669.t1 | AT5G66670 | 40.997 | 361 | 203 | 2 | 19 | 369 | 32 | 392 | 3.24e-89 | 275 |
| MS.gene056669.t1 | AT5G66660 | 40.104 | 384 | 217 | 3 | 2 | 372 | 13 | 396 | 7.52e-86 | 266 |
| MS.gene056669.t1 | AT3G28270 | 32.044 | 362 | 219 | 8 | 29 | 368 | 16 | 372 | 5.43e-41 | 148 |
| MS.gene056669.t1 | AT3G28270 | 32.044 | 362 | 219 | 8 | 29 | 368 | 16 | 372 | 5.43e-41 | 148 |
| MS.gene056669.t1 | AT3G28290 | 29.144 | 374 | 235 | 10 | 23 | 368 | 9 | 380 | 4.08e-37 | 138 |
| MS.gene056669.t1 | AT3G28300 | 29.144 | 374 | 235 | 10 | 23 | 368 | 9 | 380 | 4.08e-37 | 138 |
| MS.gene056669.t1 | AT3G57100 | 26.019 | 319 | 206 | 8 | 66 | 368 | 43 | 347 | 5.21e-25 | 104 |
| MS.gene056669.t1 | AT3G28320 | 25.949 | 316 | 202 | 9 | 81 | 368 | 2 | 313 | 9.87e-21 | 92.0 |
Find 48 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAGAATGTTGAAGATTT+AGG | 0.197144 | 4.3:+36166404 | MS.gene056669:CDS |
| CAACCAGCTCAAACAATTCT+TGG | 0.249155 | 4.3:-36165692 | None:intergenic |
| GTTGATGACTTGATTTGTTC+TGG | 0.284311 | 4.3:-36165538 | None:intergenic |
| GCTGCTAAGGCTGCAGCAAC+AGG | 0.300350 | 4.3:-36166113 | None:intergenic |
| CATATAATTCTTCAAATGTT+AGG | 0.312042 | 4.3:-36166500 | None:intergenic |
| CTATCGGATCGATGGGAAAA+TGG | 0.315755 | 4.3:+36166150 | MS.gene056669:CDS |
| CTTGATTTGTTCTGGTTTGA+AGG | 0.342243 | 4.3:-36165530 | None:intergenic |
| TTCGTTCGAATCGCTGAAAC+AGG | 0.346256 | 4.3:+36165593 | MS.gene056669:CDS |
| GTCATCAACACTCTTGCTTC+TGG | 0.357949 | 4.3:+36165552 | MS.gene056669:CDS |
| GGAAGTTGTGAAAGTAATCT+TGG | 0.367227 | 4.3:+36165644 | MS.gene056669:CDS |
| GTATGTAGCTATCAAGGATT+TGG | 0.389708 | 4.3:+36166250 | MS.gene056669:CDS |
| ATAATTGTAGCCGTGACATT+AGG | 0.416327 | 4.3:+36166438 | MS.gene056669:CDS |
| AGAAGTGTTTGAAAAGAGCT+AGG | 0.418522 | 4.3:+36165766 | MS.gene056669:CDS |
| CGAAAACAGTTTGCAAACAT+TGG | 0.422994 | 4.3:+36165725 | MS.gene056669:CDS |
| AGCTGTGAAGATTGCAATAG+AGG | 0.437623 | 4.3:+36166355 | MS.gene056669:CDS |
| GAAAATGGATTGATTCACTT+TGG | 0.441988 | 4.3:+36166165 | MS.gene056669:CDS |
| TTCGAGGAAGAATCTGAATC+AGG | 0.454083 | 4.3:+36165825 | MS.gene056669:CDS |
| ATTGGATTTCTGCACTGCAT+TGG | 0.459448 | 4.3:+36165743 | MS.gene056669:CDS |
| AGCTGAAAGGAATTCATTCA+TGG | 0.459972 | 4.3:+36166006 | MS.gene056669:CDS |
| GAGCCAAGAATTGTTTGAGC+TGG | 0.462448 | 4.3:+36165689 | MS.gene056669:CDS |
| ATGTCTCCTTGAGATGAATC+AGG | 0.463615 | 4.3:+36165623 | MS.gene056669:CDS |
| AAAGGACAGAAAGAAGTCAT+TGG | 0.479765 | 4.3:+36166209 | MS.gene056669:CDS |
| TCGCAACAAGAATCAGCAAT+TGG | 0.488179 | 4.3:-36165794 | None:intergenic |
| TCGTGTGCTTATCGATCGGC+TGG | 0.490100 | 4.3:+36166280 | MS.gene056669:CDS |
| TGGATTGCAAGAAAGACATA+TGG | 0.495682 | 4.3:+36165664 | MS.gene056669:CDS |
| ATCGATCCCTATCGGATCGA+TGG | 0.496703 | 4.3:+36166142 | MS.gene056669:CDS |
| TTGATTTGTTCTGGTTTGAA+GGG | 0.498740 | 4.3:-36165529 | None:intergenic |
| GCTAAGGCTGCAGCAACAGG+TGG | 0.499171 | 4.3:-36166110 | None:intergenic |
| CAACTTCCTGATTCATCTCA+AGG | 0.511965 | 4.3:-36165629 | None:intergenic |
| ATTGTAGCCGTGACATTAGG+AGG | 0.521279 | 4.3:+36166441 | MS.gene056669:CDS |
| GAGGATATTAAGAAGAAACT+TGG | 0.521607 | 4.3:+36166374 | MS.gene056669:CDS |
| GATCGATGTAGCGGCTGCTA+AGG | 0.526921 | 4.3:-36166126 | None:intergenic |
| AACTATGAAATTGCATTGAA+AGG | 0.532957 | 4.3:+36166191 | MS.gene056669:CDS |
| TGATTCTTGTTGCGATTCAA+CGG | 0.537293 | 4.3:+36165802 | MS.gene056669:CDS |
| TGGTACGTATGTAGCTATCA+AGG | 0.549784 | 4.3:+36166244 | MS.gene056669:CDS |
| GCCGTGACATTAGGAGGGCT+AGG | 0.555093 | 4.3:+36166447 | MS.gene056669:CDS |
| GAAGTGTTTGAAAAGAGCTA+GGG | 0.562150 | 4.3:+36165767 | MS.gene056669:CDS |
| TGTTGCGATTCAACGGTTCG+AGG | 0.563490 | 4.3:+36165809 | MS.gene056669:CDS |
| GTCATTGGTTCAATGCAAGC+TGG | 0.571219 | 4.3:+36166224 | MS.gene056669:CDS |
| TCGATCCCTATCGGATCGAT+GGG | 0.575259 | 4.3:+36166143 | MS.gene056669:CDS |
| GCCGCTACATCGATCCCTAT+CGG | 0.584549 | 4.3:+36166134 | MS.gene056669:CDS |
| TCCTAGCCCTCCTAATGTCA+CGG | 0.589587 | 4.3:-36166448 | None:intergenic |
| AAGCTTGATAAGAAGCTGAA+AGG | 0.592917 | 4.3:+36165993 | MS.gene056669:CDS |
| ATATTCGTGTGCTTATCGAT+CGG | 0.635381 | 4.3:+36166276 | MS.gene056669:CDS |
| TCCGATAGGGATCGATGTAG+CGG | 0.650270 | 4.3:-36166135 | None:intergenic |
| TTGTAGCCGTGACATTAGGA+GGG | 0.664508 | 4.3:+36166442 | MS.gene056669:CDS |
| TGAAAGGAATTCATTCATGG+AGG | 0.690641 | 4.3:+36166009 | MS.gene056669:CDS |
| AGCTGGTGATCCTTTCACTG+AGG | 0.742418 | 4.3:+36165896 | MS.gene056669:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAACAGATTGAAAAATT+TGG | - | chr4.3:36165929-36165948 | None:intergenic | 15.0% |
| ! | AACTATGAAATTGCATTGAA+AGG | + | chr4.3:36166191-36166210 | MS.gene056669:CDS | 25.0% |
| ! | ATGAAGAATGTTGAAGATTT+AGG | + | chr4.3:36166404-36166423 | MS.gene056669:CDS | 25.0% |
| !!! | TTGAAGAATTTTAAAACAGC+TGG | + | chr4.3:36165879-36165898 | MS.gene056669:CDS | 25.0% |
| GAGGATATTAAGAAGAAACT+TGG | + | chr4.3:36166374-36166393 | MS.gene056669:CDS | 30.0% | |
| !! | GAAAATGGATTGATTCACTT+TGG | + | chr4.3:36166165-36166184 | MS.gene056669:CDS | 30.0% |
| !! | TTGATTTGTTCTGGTTTGAA+GGG | - | chr4.3:36165532-36165551 | None:intergenic | 30.0% |
| !!! | TGTTTTGATATGTTCTGTTG+TGG | + | chr4.3:36166070-36166089 | MS.gene056669:CDS | 30.0% |
| AAAGGACAGAAAGAAGTCAT+TGG | + | chr4.3:36166209-36166228 | MS.gene056669:CDS | 35.0% | |
| AAGCTTGATAAGAAGCTGAA+AGG | + | chr4.3:36165993-36166012 | MS.gene056669:CDS | 35.0% | |
| AGCTGAAAGGAATTCATTCA+TGG | + | chr4.3:36166006-36166025 | MS.gene056669:CDS | 35.0% | |
| ATAATTGTAGCCGTGACATT+AGG | + | chr4.3:36166438-36166457 | MS.gene056669:CDS | 35.0% | |
| ATATTCGTGTGCTTATCGAT+CGG | + | chr4.3:36166276-36166295 | MS.gene056669:CDS | 35.0% | |
| CGAAAACAGTTTGCAAACAT+TGG | + | chr4.3:36165725-36165744 | MS.gene056669:CDS | 35.0% | |
| GGAAGTTGTGAAAGTAATCT+TGG | + | chr4.3:36165644-36165663 | MS.gene056669:CDS | 35.0% | |
| GTATGTAGCTATCAAGGATT+TGG | + | chr4.3:36166250-36166269 | MS.gene056669:CDS | 35.0% | |
| TGAAAGGAATTCATTCATGG+AGG | + | chr4.3:36166009-36166028 | MS.gene056669:CDS | 35.0% | |
| TGATTCTTGTTGCGATTCAA+CGG | + | chr4.3:36165802-36165821 | MS.gene056669:CDS | 35.0% | |
| TGGATTGCAAGAAAGACATA+TGG | + | chr4.3:36165664-36165683 | MS.gene056669:CDS | 35.0% | |
| ! | GAAGTGTTTGAAAAGAGCTA+GGG | + | chr4.3:36165767-36165786 | MS.gene056669:CDS | 35.0% |
| !! | AGAAGTGTTTGAAAAGAGCT+AGG | + | chr4.3:36165766-36165785 | MS.gene056669:CDS | 35.0% |
| !! | CTTGATTTGTTCTGGTTTGA+AGG | - | chr4.3:36165533-36165552 | None:intergenic | 35.0% |
| !! | GTTGATGACTTGATTTGTTC+TGG | - | chr4.3:36165541-36165560 | None:intergenic | 35.0% |
| AGCTGTGAAGATTGCAATAG+AGG | + | chr4.3:36166355-36166374 | MS.gene056669:CDS | 40.0% | |
| ATGTCTCCTTGAGATGAATC+AGG | + | chr4.3:36165623-36165642 | MS.gene056669:CDS | 40.0% | |
| ATTGGATTTCTGCACTGCAT+TGG | + | chr4.3:36165743-36165762 | MS.gene056669:CDS | 40.0% | |
| CAACCAGCTCAAACAATTCT+TGG | - | chr4.3:36165695-36165714 | None:intergenic | 40.0% | |
| CAACTTCCTGATTCATCTCA+AGG | - | chr4.3:36165632-36165651 | None:intergenic | 40.0% | |
| TCGCAACAAGAATCAGCAAT+TGG | - | chr4.3:36165797-36165816 | None:intergenic | 40.0% | |
| TGGAAAAACTCCTCAGTGAA+AGG | - | chr4.3:36165909-36165928 | None:intergenic | 40.0% | |
| TGGTACGTATGTAGCTATCA+AGG | + | chr4.3:36166244-36166263 | MS.gene056669:CDS | 40.0% | |
| TTCGAGGAAGAATCTGAATC+AGG | + | chr4.3:36165825-36165844 | MS.gene056669:CDS | 40.0% | |
| ! | ATTTTCCCATCGATCCGATA+GGG | - | chr4.3:36166151-36166170 | None:intergenic | 40.0% |
| ! | GATTTTGCAAGCTGCTTCAT+AGG | - | chr4.3:36165481-36165500 | None:intergenic | 40.0% |
| ATTGTAGCCGTGACATTAGG+AGG | + | chr4.3:36166441-36166460 | MS.gene056669:CDS | 45.0% | |
| CTATCGGATCGATGGGAAAA+TGG | + | chr4.3:36166150-36166169 | MS.gene056669:CDS | 45.0% | |
| GAGCCAAGAATTGTTTGAGC+TGG | + | chr4.3:36165689-36165708 | MS.gene056669:CDS | 45.0% | |
| GTCATCAACACTCTTGCTTC+TGG | + | chr4.3:36165552-36165571 | MS.gene056669:CDS | 45.0% | |
| TTCGTTCGAATCGCTGAAAC+AGG | + | chr4.3:36165593-36165612 | MS.gene056669:CDS | 45.0% | |
| TTGTAGCCGTGACATTAGGA+GGG | + | chr4.3:36166442-36166461 | MS.gene056669:CDS | 45.0% | |
| ! | CATTTTCCCATCGATCCGAT+AGG | - | chr4.3:36166152-36166171 | None:intergenic | 45.0% |
| !! | GTCATTGGTTCAATGCAAGC+TGG | + | chr4.3:36166224-36166243 | MS.gene056669:CDS | 45.0% |
| AGCTGGTGATCCTTTCACTG+AGG | + | chr4.3:36165896-36165915 | MS.gene056669:CDS | 50.0% | |
| ATCGATCCCTATCGGATCGA+TGG | + | chr4.3:36166142-36166161 | MS.gene056669:CDS | 50.0% | |
| TCCGATAGGGATCGATGTAG+CGG | - | chr4.3:36166138-36166157 | None:intergenic | 50.0% | |
| TCCTAGCCCTCCTAATGTCA+CGG | - | chr4.3:36166451-36166470 | None:intergenic | 50.0% | |
| TCGATCCCTATCGGATCGAT+GGG | + | chr4.3:36166143-36166162 | MS.gene056669:CDS | 50.0% | |
| TGTTGCGATTCAACGGTTCG+AGG | + | chr4.3:36165809-36165828 | MS.gene056669:CDS | 50.0% | |
| GATCGATGTAGCGGCTGCTA+AGG | - | chr4.3:36166129-36166148 | None:intergenic | 55.0% | |
| GCCGCTACATCGATCCCTAT+CGG | + | chr4.3:36166134-36166153 | MS.gene056669:CDS | 55.0% | |
| TCGTGTGCTTATCGATCGGC+TGG | + | chr4.3:36166280-36166299 | MS.gene056669:CDS | 55.0% | |
| GCCGTGACATTAGGAGGGCT+AGG | + | chr4.3:36166447-36166466 | MS.gene056669:CDS | 60.0% | |
| GCTAAGGCTGCAGCAACAGG+TGG | - | chr4.3:36166113-36166132 | None:intergenic | 60.0% | |
| GCTGCTAAGGCTGCAGCAAC+AGG | - | chr4.3:36166116-36166135 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 36165387 | 36166511 | 36165387 | ID=MS.gene056669 |
| chr4.3 | mRNA | 36165387 | 36166511 | 36165387 | ID=MS.gene056669.t1;Parent=MS.gene056669 |
| chr4.3 | exon | 36165387 | 36166511 | 36165387 | ID=MS.gene056669.t1.exon1;Parent=MS.gene056669.t1 |
| chr4.3 | CDS | 36165387 | 36166511 | 36165387 | ID=cds.MS.gene056669.t1;Parent=MS.gene056669.t1 |
| Gene Sequence |
| Protein sequence |
