AlfalfaGEDB | MS.gene056850

M. sativa cultivar XinJiangDaYe / MS.gene056850

KEGG: K02867
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056850.t1	XP_013468029.1	100	149	0	0	1	149	1	149	6.50E-77	296.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056850.t1	Q9UTK2	51.9	135	65	0	11	145	9	143	5.8e-34	145.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056850.t1	A0A072VKA9	100.0	149	0	0	1	149	1	149	4.7e-77	296.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene045480	MS.gene056850	PPI
MS.gene006700	MS.gene056850	PPI
MS.gene73862	MS.gene056850	PPI
MS.gene031594	MS.gene056850	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056850.t1	MTR_1g060610	100.000	149	0	0	1	149	1	149	4.81e-109	306
MS.gene056850.t1	MTR_3g080800	44.286	140	75	2	5	144	66	202	1.26e-28	105

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056850.t1	AT4G35490	85.315	143	21	0	7	149	13	155	1.15e-91	263
MS.gene056850.t1	AT4G35490	85.315	143	21	0	7	149	13	155	1.15e-91	263
MS.gene056850.t1	AT1G32990	44.286	140	75	2	5	144	72	208	7.44e-28	103

Find 54 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCATTTCTAAATCCATTAT+TGG	0.175466	1.1:+46126934	MS.gene056850:CDS
GTAACAGCCATTGGTGTATC+AGG	0.275383	1.1:-46126709	None:intergenic
CGATACCCGCAGCTTTCTTC+AGG	0.313010	1.1:-46126795	None:intergenic
GCTCCTAGCTGTACCAATAA+TGG	0.322397	1.1:-46126947	None:intergenic
ACAGTCTGATCCTTACTTGC+AGG	0.357839	1.1:+46126897	MS.gene056850:CDS
ACCAATAATGGATTTAGAAA+TGG	0.398795	1.1:-46126935	None:intergenic
GAAAGCTGCGGGTATCGAAT+TGG	0.398936	1.1:+46126801	MS.gene056850:CDS
GGAGGTGGCGGTCACATGAC+CGG	0.412632	1.1:-46126836	None:intergenic
ATTGTCGCTGCTACCACTTT+CGG	0.429301	1.1:-46126562	None:intergenic
AGTGGCATACCCTGCAAGTA+AGG	0.435747	1.1:-46126907	None:intergenic
AACGATGAGCGTTCCGAAAG+TGG	0.442624	1.1:+46126549	None:intergenic
CCTCCTGTGGGACCTGCGCT+AGG	0.444053	1.1:+46126622	MS.gene056850:CDS
CTCCAGCTGGAACTCTCAAC+CGG	0.454096	1.1:-46126585	None:intergenic
TCCAGCTGGAGGAGCACGTC+CGG	0.454983	1.1:+46126597	MS.gene056850:CDS
AGCACGTCCGGCGCCTCCTG+TGG	0.466575	1.1:+46126609	MS.gene056850:CDS
AAAGCTGCGGGTATCGAATT+GGG	0.473529	1.1:+46126802	MS.gene056850:CDS
AGTCTTTGCAGAAAGCCATA+AGG	0.492679	1.1:-46126660	None:intergenic
GCTGTTATTGTAACAGCCAT+TGG	0.492932	1.1:-46126718	None:intergenic
GGCGGTCACATGACCGGGAC+GGG	0.494244	1.1:-46126830	None:intergenic
TCAGTACCGACTGAACCTTA+TGG	0.497875	1.1:+46126645	MS.gene056850:CDS
AAGTGGTAGCAGCGACAATC+CGG	0.509428	1.1:+46126566	MS.gene056850:CDS
GCCGGACGTGCTCCTCCAGC+TGG	0.512277	1.1:-46126598	None:intergenic
GCACGTCCGGCGCCTCCTGT+GGG	0.517535	1.1:+46126610	MS.gene056850:CDS
CATACACGTGCCGAAGTGAC+AGG	0.517596	1.1:-46126858	None:intergenic
GAGGTGGCGGTCACATGACC+GGG	0.519759	1.1:-46126835	None:intergenic
CCGAAGTGACAGGGAGGTGG+CGG	0.522973	1.1:-46126848	None:intergenic
TGTTACAATAACAGCTTACA+AGG	0.549953	1.1:+46126726	MS.gene056850:CDS
TGACCTAGCGCAGGTCCCAC+AGG	0.552959	1.1:-46126625	None:intergenic
GCAGGTCCCACAGGAGGCGC+CGG	0.567732	1.1:-46126616	None:intergenic
ATTGGTACAGCTAGGAGCAT+GGG	0.571876	1.1:+46126952	MS.gene056850:CDS
CCGCCACCTCCCTGTCACTT+CGG	0.584871	1.1:+46126848	MS.gene056850:CDS
TGGTACCTGAAGAAAGCTGC+GGG	0.584881	1.1:+46126790	MS.gene056850:CDS
ATCCGGTTGAGAGTTCCAGC+TGG	0.592293	1.1:+46126583	MS.gene056850:CDS
TATTGGTACAGCTAGGAGCA+TGG	0.593721	1.1:+46126951	MS.gene056850:CDS
CAGTCTGATCCTTACTTGCA+GGG	0.597928	1.1:+46126898	MS.gene056850:CDS
GTGCCGAAGTGACAGGGAGG+TGG	0.602083	1.1:-46126851	None:intergenic
TTAAATCTCCTTCCGTCTCG+TGG	0.602388	1.1:+46126770	MS.gene056850:CDS
TTCAGGTACCACGAGACGGA+AGG	0.605506	1.1:-46126778	None:intergenic
TGGCGGTCACATGACCGGGA+CGG	0.607191	1.1:-46126831	None:intergenic
TTTCTTCAGGTACCACGAGA+CGG	0.610355	1.1:-46126782	None:intergenic
AGTCGGTACTGACCTAGCGC+AGG	0.626095	1.1:-46126634	None:intergenic
GAATTGGGAAGTACCCGTCC+CGG	0.626450	1.1:+46126817	MS.gene056850:CDS
AATCCATTATTGGTACAGCT+AGG	0.638443	1.1:+46126944	MS.gene056850:CDS
CCTAGCGCAGGTCCCACAGG+AGG	0.638505	1.1:-46126622	None:intergenic
ATACACGTGCCGAAGTGACA+GGG	0.645886	1.1:-46126857	None:intergenic
AGAAAGCCATAAGGTTCAGT+CGG	0.648161	1.1:-46126651	None:intergenic
TCTGCAAAGACTTCAACGCA+CGG	0.650245	1.1:+46126671	MS.gene056850:CDS
AGATTTAACAGTGAACTCAA+AGG	0.651355	1.1:-46126755	None:intergenic
GATTTAGAAATGGACTCAAG+TGG	0.655714	1.1:-46126925	None:intergenic
ATTCAAGCCTGATACACCAA+TGG	0.658568	1.1:+46126702	MS.gene056850:CDS
GTGGTACCTGAAGAAAGCTG+CGG	0.662466	1.1:+46126789	MS.gene056850:CDS
CGGTTGAGAGTTCCAGCTGG+AGG	0.663118	1.1:+46126586	MS.gene056850:CDS
GCACGTGTATGAGATCGCTA+AGG	0.664058	1.1:+46126870	MS.gene056850:CDS
CACGTGCCGAAGTGACAGGG+AGG	0.790794	1.1:-46126854	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCCATTTCTAAATCCATTAT+TGG	+	chr1.1:46126934-46126953	MS.gene056850:CDS	25.0%
!!!	ACCAATAATGGATTTAGAAA+TGG	-	chr1.1:46126938-46126957	None:intergenic	25.0%
	AGATTTAACAGTGAACTCAA+AGG	-	chr1.1:46126758-46126777	None:intergenic	30.0%
	TGTTACAATAACAGCTTACA+AGG	+	chr1.1:46126726-46126745	MS.gene056850:CDS	30.0%
	AATCCATTATTGGTACAGCT+AGG	+	chr1.1:46126944-46126963	MS.gene056850:CDS	35.0%
!	GATTTAGAAATGGACTCAAG+TGG	-	chr1.1:46126928-46126947	None:intergenic	35.0%
	AGAAAGCCATAAGGTTCAGT+CGG	-	chr1.1:46126654-46126673	None:intergenic	40.0%
	AGTCTTTGCAGAAAGCCATA+AGG	-	chr1.1:46126663-46126682	None:intergenic	40.0%
	ATTCAAGCCTGATACACCAA+TGG	+	chr1.1:46126702-46126721	MS.gene056850:CDS	40.0%
	GCTGTTATTGTAACAGCCAT+TGG	-	chr1.1:46126721-46126740	None:intergenic	40.0%
	ACAGTCTGATCCTTACTTGC+AGG	+	chr1.1:46126897-46126916	MS.gene056850:CDS	45.0%
	ATTGTCGCTGCTACCACTTT+CGG	-	chr1.1:46126565-46126584	None:intergenic	45.0%
	CAGTCTGATCCTTACTTGCA+GGG	+	chr1.1:46126898-46126917	MS.gene056850:CDS	45.0%
	GCTCCTAGCTGTACCAATAA+TGG	-	chr1.1:46126950-46126969	None:intergenic	45.0%
	GTAACAGCCATTGGTGTATC+AGG	-	chr1.1:46126712-46126731	None:intergenic	45.0%
	TATTGGTACAGCTAGGAGCA+TGG	+	chr1.1:46126951-46126970	MS.gene056850:CDS	45.0%
	TCTGCAAAGACTTCAACGCA+CGG	+	chr1.1:46126671-46126690	MS.gene056850:CDS	45.0%
	TTAAATCTCCTTCCGTCTCG+TGG	+	chr1.1:46126770-46126789	MS.gene056850:CDS	45.0%
	TTTCTTCAGGTACCACGAGA+CGG	-	chr1.1:46126785-46126804	None:intergenic	45.0%
!	ATTGGTACAGCTAGGAGCAT+GGG	+	chr1.1:46126952-46126971	MS.gene056850:CDS	45.0%
!!	AAAGCTGCGGGTATCGAATT+GGG	+	chr1.1:46126802-46126821	MS.gene056850:CDS	45.0%
!!	TCAGTACCGACTGAACCTTA+TGG	+	chr1.1:46126645-46126664	MS.gene056850:CDS	45.0%
	AAGTGGTAGCAGCGACAATC+CGG	+	chr1.1:46126566-46126585	MS.gene056850:CDS	50.0%
	AGTGGCATACCCTGCAAGTA+AGG	-	chr1.1:46126910-46126929	None:intergenic	50.0%
	ATACACGTGCCGAAGTGACA+GGG	-	chr1.1:46126860-46126879	None:intergenic	50.0%
	GCACGTGTATGAGATCGCTA+AGG	+	chr1.1:46126870-46126889	MS.gene056850:CDS	50.0%
	GTGGTACCTGAAGAAAGCTG+CGG	+	chr1.1:46126789-46126808	MS.gene056850:CDS	50.0%
	TGGTACCTGAAGAAAGCTGC+GGG	+	chr1.1:46126790-46126809	MS.gene056850:CDS	50.0%
!!	GAAAGCTGCGGGTATCGAAT+TGG	+	chr1.1:46126801-46126820	MS.gene056850:CDS	50.0%
	ATCCGGTTGAGAGTTCCAGC+TGG	+	chr1.1:46126583-46126602	MS.gene056850:CDS	55.0%
	CATACACGTGCCGAAGTGAC+AGG	-	chr1.1:46126861-46126880	None:intergenic	55.0%
	CGATACCCGCAGCTTTCTTC+AGG	-	chr1.1:46126798-46126817	None:intergenic	55.0%
	CTCCAGCTGGAACTCTCAAC+CGG	-	chr1.1:46126588-46126607	None:intergenic	55.0%
	TTCAGGTACCACGAGACGGA+AGG	-	chr1.1:46126781-46126800	None:intergenic	55.0%
!!	GAATTGGGAAGTACCCGTCC+CGG	+	chr1.1:46126817-46126836	MS.gene056850:CDS	55.0%
	CGGTTGAGAGTTCCAGCTGG+AGG	+	chr1.1:46126586-46126605	MS.gene056850:CDS	60.0%
!!	AGTCGGTACTGACCTAGCGC+AGG	-	chr1.1:46126637-46126656	None:intergenic	60.0%
	CACGTGCCGAAGTGACAGGG+AGG	-	chr1.1:46126857-46126876	None:intergenic	65.0%
	CCGAAGTGACAGGGAGGTGG+CGG	-	chr1.1:46126851-46126870	None:intergenic	65.0%
	GTGCCGAAGTGACAGGGAGG+TGG	-	chr1.1:46126854-46126873	None:intergenic	65.0%
	TGACCTAGCGCAGGTCCCAC+AGG	-	chr1.1:46126628-46126647	None:intergenic	65.0%
	TGGCGGTCACATGACCGGGA+CGG	-	chr1.1:46126834-46126853	None:intergenic	65.0%
!	CCGCCACCTCCCTGTCACTT+CGG	+	chr1.1:46126848-46126867	MS.gene056850:CDS	65.0%
!	GAGGTGGCGGTCACATGACC+GGG	-	chr1.1:46126838-46126857	None:intergenic	65.0%
!	GGAGGTGGCGGTCACATGAC+CGG	-	chr1.1:46126839-46126858	None:intergenic	65.0%
!	TCCAGCTGGAGGAGCACGTC+CGG	+	chr1.1:46126597-46126616	MS.gene056850:CDS	65.0%
	CCTAGCGCAGGTCCCACAGG+AGG	-	chr1.1:46126625-46126644	None:intergenic	70.0%
	CCTCCTGTGGGACCTGCGCT+AGG	+	chr1.1:46126622-46126641	MS.gene056850:CDS	70.0%
	GGCGGTCACATGACCGGGAC+GGG	-	chr1.1:46126833-46126852	None:intergenic	70.0%
	AGCACGTCCGGCGCCTCCTG+TGG	+	chr1.1:46126609-46126628	MS.gene056850:CDS	75.0%
	GCACGTCCGGCGCCTCCTGT+GGG	+	chr1.1:46126610-46126629	MS.gene056850:CDS	75.0%
	GCCGGACGTGCTCCTCCAGC+TGG	-	chr1.1:46126601-46126620	None:intergenic	75.0%
!	GCAGGTCCCACAGGAGGCGC+CGG	-	chr1.1:46126619-46126638	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	46126553	46127002	46126553	ID=MS.gene056850
chr1.1	mRNA	46126553	46127002	46126553	ID=MS.gene056850.t1;Parent=MS.gene056850
chr1.1	exon	46126553	46127002	46126553	ID=MS.gene056850.t1.exon1;Parent=MS.gene056850.t1
chr1.1	CDS	46126553	46127002	46126553	ID=cds.MS.gene056850.t1;Parent=MS.gene056850.t1

Gene Sequence

>MS.gene056850

ATGAGCGTTCCGAAAGTGGTAGCAGCGACAATCCGGTTGAGAGTTCCAGCTGGAGGAGCACGTCCGGCGCCTCCTGTGGGACCTGCGCTAGGTCAGTACCGACTGAACCTTATGGCTTTCTGCAAAGACTTCAACGCACGGACTCAGAAATTCAAGCCTGATACACCAATGGCTGTTACAATAACAGCTTACAAGGACAACACCTTTGAGTTCACTGTTAAATCTCCTTCCGTCTCGTGGTACCTGAAGAAAGCTGCGGGTATCGAATTGGGAAGTACCCGTCCCGGTCATGTGACCGCCACCTCCCTGTCACTTCGGCACGTGTATGAGATCGCTAAGGTGAAACAGTCTGATCCTTACTTGCAGGGTATGCCACTTGAGTCCATTTCTAAATCCATTATTGGTACAGCTAGGAGCATGGGAATCAAAATTGTCAAAGATCTTGAATGA

Protein sequence

>MS.gene056850.t1

MSVPKVVAATIRLRVPAGGARPAPPVGPALGQYRLNLMAFCKDFNARTQKFKPDTPMAVTITAYKDNTFEFTVKSPSVSWYLKKAAGIELGSTRPGHVTATSLSLRHVYEIAKVKQSDPYLQGMPLESISKSIIGTARSMGIKIVKDLE