AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056957

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056957.t1 MTR_4g113140 79.951 409 33 3 1 360 1 409 0.0 619
MS.gene056957.t1 MTR_1g093080 37.134 307 145 8 29 287 2 308 1.10e-48 168
MS.gene056957.t1 MTR_5g054300 39.748 317 135 13 30 309 12 309 3.35e-47 164
MS.gene056957.t1 MTR_5g017980 37.413 286 121 9 6 264 10 264 3.49e-37 137
MS.gene056957.t1 MTR_1g093080 35.769 260 123 6 29 244 2 261 3.56e-37 135
MS.gene056957.t1 MTR_4g086835 68.421 95 25 2 194 287 274 364 1.29e-35 134
MS.gene056957.t1 MTR_4g086835 41.667 132 62 5 4 121 6 136 9.89e-19 87.4
MS.gene056957.t1 MTR_5g017980 37.063 286 120 9 6 264 10 262 1.35e-35 132
MS.gene056957.t1 MTR_5g017980 37.413 286 120 10 6 264 10 263 1.54e-35 132
MS.gene056957.t1 MTR_4g086835 68.421 95 24 3 194 287 274 363 6.34e-34 130
MS.gene056957.t1 MTR_4g086835 41.667 132 62 5 4 121 6 136 9.38e-19 87.4
MS.gene056957.t1 MTR_1g093080 29.956 227 117 5 33 217 8 234 3.79e-20 89.0
MS.gene056957.t1 MTR_1g080330 47.059 85 43 1 185 269 166 248 2.40e-15 75.9
MS.gene056957.t1 MTR_1g080330 47.059 85 43 1 185 269 166 248 4.50e-15 76.3
MS.gene056957.t1 MTR_2g450070 38.462 104 56 3 194 290 213 315 2.05e-14 75.1
MS.gene056957.t1 MTR_2g450070 38.462 104 56 3 194 290 213 315 2.25e-14 74.7
MS.gene056957.t1 MTR_7g098250 50.633 79 37 1 194 272 262 338 6.27e-14 73.2
MS.gene056957.t1 MTR_1g053830 49.180 61 31 0 196 256 20 80 6.84e-14 67.4
MS.gene056957.t1 MTR_7g115530 56.667 60 26 0 194 253 218 277 4.87e-13 69.3
MS.gene056957.t1 MTR_4g131580 50.000 68 33 1 194 261 200 266 5.54e-13 70.5
MS.gene056957.t1 MTR_7g115530 56.667 60 26 0 194 253 218 277 7.12e-13 69.3
MS.gene056957.t1 MTR_7g089010 50.794 63 30 1 190 252 87 148 8.38e-13 68.6
MS.gene056957.t1 MTR_7g115530 56.667 60 26 0 194 253 218 277 8.60e-13 68.9
MS.gene056957.t1 MTR_4g064730 41.758 91 51 2 185 275 122 210 1.06e-12 68.6
MS.gene056957.t1 MTR_7g115530 56.667 60 26 0 194 253 218 277 1.36e-12 68.9
MS.gene056957.t1 MTR_7g088070 52.941 68 31 1 193 259 49 116 1.58e-12 67.4
MS.gene056957.t1 MTR_1g053835 43.750 96 36 2 161 256 243 320 1.61e-12 68.6
MS.gene056957.t1 MTR_1g013170 55.738 61 26 1 194 254 191 250 2.97e-12 68.2
MS.gene056957.t1 MTR_7g088070 55.738 61 27 0 193 253 49 109 3.28e-12 66.6
MS.gene056957.t1 MTR_2g023580 61.538 52 20 0 196 247 57 108 3.45e-12 65.9
MS.gene056957.t1 MTR_5g014040 34.058 138 80 4 123 252 234 368 4.23e-12 67.8
MS.gene056957.t1 MTR_5g041350 53.571 56 26 0 193 248 14 69 4.71e-12 67.0
MS.gene056957.t1 MTR_4g121020 53.846 65 29 1 194 258 215 278 4.79e-12 67.8
MS.gene056957.t1 MTR_4g121020 53.846 65 29 1 194 258 111 174 4.91e-12 67.4
MS.gene056957.t1 MTR_4g121020 53.846 65 29 1 194 258 215 278 4.91e-12 67.8
MS.gene056957.t1 MTR_2g086450 49.254 67 34 0 193 259 44 110 7.24e-12 66.6
MS.gene056957.t1 MTR_6g032990 44.304 79 39 2 193 267 41 118 1.02e-11 66.2
MS.gene056957.t1 MTR_8g079940 48.571 70 35 1 183 252 198 266 1.12e-11 66.2
MS.gene056957.t1 MTR_5g027440 40.196 102 48 2 194 293 25 115 1.44e-11 64.7
MS.gene056957.t1 MTR_0223s0040 61.224 49 19 0 199 247 23 71 1.55e-11 64.7
MS.gene056957.t1 MTR_2g034960 43.678 87 42 2 194 274 211 296 1.89e-11 65.9
MS.gene056957.t1 MTR_2g027860 50.746 67 33 0 194 260 49 115 2.12e-11 64.7
MS.gene056957.t1 MTR_1g112370 59.184 49 20 0 199 247 27 75 2.86e-11 64.3
MS.gene056957.t1 MTR_1g090670 58.182 55 23 0 194 248 44 98 4.13e-11 63.5
MS.gene056957.t1 MTR_1g032570 50.725 69 31 2 194 260 200 267 4.30e-11 64.7
MS.gene056957.t1 MTR_1g032570 50.725 69 31 2 194 260 131 198 4.32e-11 64.7
MS.gene056957.t1 MTR_4g098870 45.455 66 35 1 194 259 310 374 4.61e-11 64.3
MS.gene056957.t1 MTR_4g098870 45.455 66 35 1 194 259 330 394 4.67e-11 64.7
MS.gene056957.t1 MTR_2g027800 58.182 55 23 0 194 248 39 93 4.99e-11 63.5
MS.gene056957.t1 MTR_7g069660 57.143 49 21 0 199 247 76 124 5.28e-11 63.5
MS.gene056957.t1 MTR_4g098870 45.455 66 35 1 194 259 330 394 5.66e-11 64.3
MS.gene056957.t1 MTR_4g098870 45.455 66 35 1 194 259 349 413 5.71e-11 64.3
MS.gene056957.t1 MTR_2g027800 58.182 55 23 0 194 248 39 93 5.76e-11 63.2
MS.gene056957.t1 MTR_4g131570 47.059 68 35 1 194 261 203 269 5.88e-11 64.3
MS.gene056957.t1 MTR_4g098870 45.455 66 35 1 194 259 310 374 5.89e-11 64.3
MS.gene056957.t1 MTR_7g115530 59.615 52 21 0 194 245 218 269 6.88e-11 62.8
MS.gene056957.t1 MTR_7g093030 53.704 54 25 0 194 247 212 265 9.17e-11 62.4
MS.gene056957.t1 MTR_5g029470 41.975 81 38 2 176 247 192 272 9.61e-11 63.2
MS.gene056957.t1 MTR_3g064840 49.231 65 33 0 196 260 276 340 9.91e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056957.t1 AT2G03500 52.000 375 104 14 1 317 1 357 6.44e-103 310
MS.gene056957.t1 AT3G25790 36.792 318 141 8 19 292 1 302 7.75e-52 176
MS.gene056957.t1 AT1G68670 36.648 352 126 13 32 310 13 340 3.42e-51 174
MS.gene056957.t1 AT1G25550 40.000 295 138 10 18 275 2 294 3.89e-50 171
MS.gene056957.t1 AT1G13300 38.621 290 119 10 19 275 1 264 5.38e-47 163
MS.gene056957.t1 AT1G25550 38.462 260 125 8 18 244 2 259 5.66e-38 137
MS.gene056957.t1 AT3G25790 35.496 262 117 6 19 244 1 246 2.36e-37 135
MS.gene056957.t1 AT1G49560 37.255 255 134 8 32 273 27 268 5.67e-32 123
MS.gene056957.t1 AT1G49560 36.444 225 122 6 32 244 27 242 4.84e-25 102
MS.gene056957.t1 AT4G37180 78.947 57 12 0 196 252 212 268 2.73e-23 99.8
MS.gene056957.t1 AT4G37180 78.947 57 12 0 196 252 219 275 3.16e-23 99.8
MS.gene056957.t1 AT4G37180 31.900 279 115 7 7 244 16 260 6.03e-22 94.7
MS.gene056957.t1 AT4G28610 53.750 80 36 1 193 272 224 302 5.66e-17 82.0
MS.gene056957.t1 AT5G05090 35.252 139 70 4 190 325 77 198 6.65e-15 74.3
MS.gene056957.t1 AT2G20400 51.899 79 37 1 194 272 231 308 2.56e-14 73.9
MS.gene056957.t1 AT2G20400 51.899 79 36 1 194 272 231 307 3.57e-14 73.6
MS.gene056957.t1 AT5G18240 46.237 93 37 3 169 248 7 99 3.82e-13 70.5
MS.gene056957.t1 AT5G18240 46.237 93 37 3 169 248 7 99 3.82e-13 70.5
MS.gene056957.t1 AT2G40970 34.400 125 67 2 190 314 101 210 4.37e-13 68.9
MS.gene056957.t1 AT5G18240 46.237 93 37 3 169 248 7 99 4.40e-13 70.5
MS.gene056957.t1 AT5G18240 46.237 93 37 3 169 248 7 99 4.56e-13 70.5
MS.gene056957.t1 AT5G18240 46.237 93 37 3 169 248 7 99 4.56e-13 70.5
MS.gene056957.t1 AT3G46640 40.659 91 52 2 187 277 137 225 4.74e-13 69.7
MS.gene056957.t1 AT3G46640 40.659 91 52 2 187 277 137 225 4.74e-13 69.7
MS.gene056957.t1 AT3G10760 50.794 63 30 1 190 252 101 162 6.44e-13 69.3
MS.gene056957.t1 AT3G46640 42.045 88 49 2 190 277 140 225 6.67e-13 69.3
MS.gene056957.t1 AT2G06020 58.182 55 23 0 194 248 86 140 7.34e-13 68.9
MS.gene056957.t1 AT5G59570 29.944 177 98 5 83 252 41 198 3.90e-12 66.6
MS.gene056957.t1 AT5G59570 29.944 177 98 5 83 252 41 198 3.90e-12 66.6
MS.gene056957.t1 AT3G04030 55.357 56 25 0 193 248 44 99 4.71e-12 67.0
MS.gene056957.t1 AT3G04030 55.357 56 25 0 193 248 44 99 5.13e-12 67.0
MS.gene056957.t1 AT2G02060 59.184 49 20 0 199 247 34 82 6.16e-12 65.5
MS.gene056957.t1 AT2G02060 59.184 49 20 0 199 247 34 82 6.16e-12 65.5
MS.gene056957.t1 AT5G29000 48.571 70 36 0 193 262 230 299 7.63e-12 66.6
MS.gene056957.t1 AT5G29000 48.571 70 36 0 193 262 187 256 7.92e-12 66.2
MS.gene056957.t1 AT5G29000 48.571 70 36 0 193 262 187 256 7.92e-12 66.2
MS.gene056957.t1 AT2G38300 55.769 52 23 0 196 247 56 107 1.02e-11 65.9
MS.gene056957.t1 AT2G01760 51.562 64 30 1 194 257 135 197 1.21e-11 65.5
MS.gene056957.t1 AT3G13040 53.333 60 28 0 194 253 241 300 1.35e-11 65.9
MS.gene056957.t1 AT3G13040 53.333 60 28 0 194 253 241 300 1.35e-11 65.9
MS.gene056957.t1 AT3G13040 53.333 60 28 0 194 253 241 300 1.35e-11 65.9
MS.gene056957.t1 AT2G01760 51.562 64 30 1 194 257 228 290 1.48e-11 65.9
MS.gene056957.t1 AT3G04450 47.692 65 34 0 189 253 233 297 1.59e-11 65.1
MS.gene056957.t1 AT5G29000 43.210 81 46 0 180 260 217 297 1.61e-11 65.1
MS.gene056957.t1 AT2G01760 51.562 64 30 1 194 257 200 262 1.67e-11 65.5
MS.gene056957.t1 AT5G06800 50.000 62 31 0 192 253 190 251 1.72e-11 64.7
MS.gene056957.t1 AT3G04450 44.595 74 41 0 189 262 233 306 1.92e-11 65.5
MS.gene056957.t1 AT3G04450 44.595 74 41 0 189 262 193 266 2.07e-11 65.1
MS.gene056957.t1 AT3G04450 44.595 74 41 0 189 262 193 266 2.07e-11 65.1
MS.gene056957.t1 AT1G67710 38.384 99 50 1 165 252 79 177 2.64e-11 65.1
MS.gene056957.t1 AT1G67710 38.384 99 50 1 165 252 88 186 2.76e-11 65.1
MS.gene056957.t1 AT2G01060 50.000 66 33 0 194 259 15 80 2.86e-11 63.9
MS.gene056957.t1 AT5G45580 56.364 55 24 0 194 248 7 61 3.41e-11 63.5
MS.gene056957.t1 AT5G06800 50.000 62 31 0 192 253 190 251 3.50e-11 64.3
MS.gene056957.t1 AT5G06800 50.000 62 31 0 192 253 190 251 3.56e-11 64.3
MS.gene056957.t1 AT5G45580 56.364 55 24 0 194 248 23 77 3.65e-11 63.5
MS.gene056957.t1 AT5G58080 50.847 59 28 1 194 252 194 251 3.83e-11 65.1
MS.gene056957.t1 AT1G67710 38.384 99 50 1 165 252 153 251 3.99e-11 64.7
MS.gene056957.t1 AT1G14600 57.143 49 21 0 199 247 28 76 5.44e-11 62.8
MS.gene056957.t1 AT3G16857 54.237 59 26 1 194 252 167 224 5.71e-11 64.3
MS.gene056957.t1 AT3G16857 54.237 59 26 1 194 252 237 294 7.32e-11 63.9
MS.gene056957.t1 AT3G16857 52.459 61 28 1 192 252 235 294 7.68e-11 63.9

Find 80 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACTCCCTTATCTGTTT+TGG 0.218983 8.4:+15437529 None:intergenic
TTGTTCCTAATTCACTTGTT+TGG 0.309917 8.4:+15438481 None:intergenic
GGATGACAAGTGCACAATTA+TGG 0.322662 8.4:-15438506 MS.gene056957:CDS
AGGGAGTTGATGAAGGTTGA+TGG 0.335126 8.4:-15437517 MS.gene056957:CDS
GGTATGAATTCTTCTAGAAT+TGG 0.343936 8.4:+15438600 None:intergenic
TGTGCTGCAGTTGCATATTC+TGG 0.345650 8.4:+15437169 None:intergenic
CATGCTGGAGAGGTAGAAAA+AGG 0.354964 8.4:-15438269 MS.gene056957:CDS
TTATTATTGAACAGAAATAT+AGG 0.370436 8.4:-15437276 MS.gene056957:intron
TCCATGTTACTGAAATCATT+TGG 0.380670 8.4:-15438956 MS.gene056957:CDS
ATGTATGTGATAAGGCAAAT+TGG 0.383284 8.4:-15438527 MS.gene056957:CDS
GAAGTTGATCATCAAGGAAA+AGG 0.397063 8.4:-15438227 MS.gene056957:CDS
CCAAGTCCAAGTGCACAAAA+TGG 0.410271 8.4:-15437238 MS.gene056957:CDS
ACAAATCTACGGTGCAAGTC+TGG 0.427901 8.4:+15438122 None:intergenic
GGAGGAACCCCTACTCTCTA+TGG 0.427913 8.4:-15437142 MS.gene056957:CDS
GATGATGCTAATTGCTTCAC+TGG 0.429693 8.4:+15438579 None:intergenic
GGGTTGGAGAGTATCGAAAA+TGG 0.429853 8.4:-15436943 MS.gene056957:CDS
GTAGAAGCTAGTGCTAGTTC+AGG 0.432544 8.4:+15438314 None:intergenic
GGAAGTAGCTGCTGCGGCGG+CGG 0.442713 8.4:+15437040 None:intergenic
ACAATTCAAACCAAACTCAT+AGG 0.443130 8.4:-15438167 MS.gene056957:CDS
GTTAATGCTCTTCAAATGCT+TGG 0.449127 8.4:-15438101 MS.gene056957:CDS
AAATAATAATGTATGTGATA+AGG 0.450078 8.4:-15438535 MS.gene056957:CDS
GGCAACACCAAAACAGATAA+GGG 0.469043 8.4:-15437536 MS.gene056957:CDS
CTGAGTTGTGATGATGCTAC+TGG 0.476767 8.4:+15438200 None:intergenic
CACATGTCTCCGGTGAACCT+TGG 0.477201 8.4:+15436971 None:intergenic
AACCCGTCTCCACATGTCTC+CGG 0.479058 8.4:+15436961 None:intergenic
CAGTAGTGTAGTACTGATCT+TGG 0.489595 8.4:+15437065 None:intergenic
TTCACCATCTGAACTAGGCT+TGG 0.491547 8.4:-15438999 MS.gene056957:CDS
CCACATTACTGCACTGCTCC+AGG 0.496026 8.4:-15437088 MS.gene056957:CDS
ATTAGCATCATCAGAAACAT+TGG 0.504933 8.4:-15438568 MS.gene056957:CDS
CAAATGCTTGGTGGATCTCA+AGG 0.514435 8.4:-15438089 MS.gene056957:intron
GATGACCACCATAGAGAGTA+GGG 0.520807 8.4:+15437134 None:intergenic
GAAGAGGATAAGAAACATGC+TGG 0.521576 8.4:-15438284 MS.gene056957:CDS
GTGTAGTACTGATCTTGGCC+TGG 0.523675 8.4:+15437070 None:intergenic
TGGCAACACCAAAACAGATA+AGG 0.524534 8.4:-15437537 MS.gene056957:CDS
GGCGGAAGTAGCTGCTGCGG+CGG 0.525740 8.4:+15437037 None:intergenic
AAGTTGATCATCAAGGAAAA+GGG 0.527682 8.4:-15438226 MS.gene056957:CDS
TGCATGCAACTTCTCACAAA+CGG 0.528281 8.4:-15438836 MS.gene056957:intron
AGAGTGAGATGATGAGAAGT+AGG 0.531857 8.4:+15437112 None:intergenic
AAAACTACTAGCTGTGCTGC+TGG 0.531932 8.4:+15437211 None:intergenic
CAAGCGTTTAGATCGAATCA+AGG 0.533730 8.4:-15438630 MS.gene056957:CDS
GAAGAGCATTAACAAATCTA+CGG 0.536877 8.4:+15438111 None:intergenic
TCCAAATGATTTCAGTAACA+TGG 0.537583 8.4:+15438955 None:intergenic
CACCGGAGACATGTGGAGAC+GGG 0.540363 8.4:-15436963 MS.gene056957:CDS
CCACACGGACCAAGGTTCAC+CGG 0.542299 8.4:-15436980 MS.gene056957:CDS
ATGGCTTCACCATCTGAACT+AGG 0.543239 8.4:-15439004 MS.gene056957:CDS
CAATCCAAGCCTAGTTCAGA+TGG 0.544635 8.4:+15438995 None:intergenic
TGAAGTTAAAAGCCATCTGC+AGG 0.544962 8.4:-15437482 MS.gene056957:intron
AATGCTCTTCAAATGCTTGG+TGG 0.549954 8.4:-15438098 MS.gene056957:CDS
ACAGATAAGGGAGTTGATGA+AGG 0.551674 8.4:-15437524 MS.gene056957:CDS
GGATGACCACCATAGAGAGT+AGG 0.564523 8.4:+15437133 None:intergenic
TAGGAAAAGCAAGAAGGTGT+TGG 0.568821 8.4:-15438148 MS.gene056957:CDS
GGATAAGAAACATGCTGGAG+AGG 0.570240 8.4:-15438279 MS.gene056957:CDS
TATGCAACTGCAGCACATGC+AGG 0.576335 8.4:-15437163 MS.gene056957:CDS
GAGAGAGAATTACCTGCAGA+TGG 0.576980 8.4:+15437470 None:intergenic
TCACCGGAGACATGTGGAGA+CGG 0.576988 8.4:-15436964 MS.gene056957:CDS
GGGAATGAAGTTGATCATCA+AGG 0.578253 8.4:-15438233 MS.gene056957:CDS
GGAGACATGTGGAGACGGGT+TGG 0.587274 8.4:-15436959 MS.gene056957:CDS
AGAAATAACTCCTCTTGTCA+AGG 0.588185 8.4:-15438347 MS.gene056957:CDS
GCTGCAGTTGCATATTCTGG+TGG 0.594788 8.4:+15437172 None:intergenic
AACTCATAGGAAAAGCAAGA+AGG 0.600912 8.4:-15438154 MS.gene056957:CDS
CTGCAGTTGCATATTCTGGT+GGG 0.605931 8.4:+15437173 None:intergenic
AGTAACATGGAGTAGCTATG+TGG 0.617118 8.4:+15438968 None:intergenic
GAACCTTGGTCCGTGTGGCG+TGG 0.619138 8.4:+15436985 None:intergenic
TGGTGCATGTGATGAGGTGG+CGG 0.620813 8.4:+15437019 None:intergenic
AACTCCTCTTGTCAAGGACA+AGG 0.622383 8.4:-15438341 MS.gene056957:CDS
GTGGCGTGGTCTTGTAGACA+TGG 0.624325 8.4:+15436999 None:intergenic
GGTGGCGGAAGTAGCTGCTG+CGG 0.626284 8.4:+15437034 None:intergenic
AGACCACGCCACACGGACCA+AGG 0.631906 8.4:-15436988 MS.gene056957:CDS
AAGACCTTGTCCTTGACAAG+AGG 0.632597 8.4:+15438337 None:intergenic
CAAGGTTCACCGGAGACATG+TGG 0.636033 8.4:-15436970 MS.gene056957:CDS
CCGGTGAACCTTGGTCCGTG+TGG 0.638348 8.4:+15436980 None:intergenic
ACATGGTGCATGTGATGAGG+TGG 0.655489 8.4:+15437016 None:intergenic
CCTGGAGCAGTGCAGTAATG+TGG 0.657609 8.4:+15437088 None:intergenic
GTGAGAAGTTGCATGCAGAG+AGG 0.660628 8.4:+15438842 None:intergenic
GTCTACAAGACCACGCCACA+CGG 0.660721 8.4:-15436995 MS.gene056957:CDS
TAGACATGGTGCATGTGATG+AGG 0.673422 8.4:+15437013 None:intergenic
GCAACTGCAGCACATGCAGG+AGG 0.687455 8.4:-15437160 MS.gene056957:CDS
TATCGAAAATGGAAAGACGT+CGG 0.689251 8.4:-15436932 MS.gene056957:CDS
GGAACCCCTACTCTCTATGG+TGG 0.690464 8.4:-15437139 MS.gene056957:CDS
ATGACCACCATAGAGAGTAG+GGG 0.721767 8.4:+15437135 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATTATATATTTAA+GGG - chr8.4:15438137-15438156 MS.gene056957:CDS 0.0%
!! TTAAAATTATTATATATTTA+AGG - chr8.4:15438136-15438155 MS.gene056957:CDS 0.0%
!! AAATAATAATGTATGTGATA+AGG - chr8.4:15437394-15437413 MS.gene056957:intron 15.0%
!! AAATTTAGTCAAATGTTATT+AGG - chr8.4:15438250-15438269 MS.gene056957:CDS 15.0%
!! AATGATAACAATAACAATAT+TGG - chr8.4:15437500-15437519 MS.gene056957:CDS 15.0%
!! ATACTATAAAAAAGAATAGA+TGG - chr8.4:15437963-15437982 MS.gene056957:intron 15.0%
!! GTTACATATAAAAGAAAAAA+AGG + chr8.4:15437135-15437154 None:intergenic 15.0%
!! TTATTATTGAACAGAAATAT+AGG - chr8.4:15438653-15438672 MS.gene056957:CDS 15.0%
!! TTTCTTTATAAAATAGTCTT+AGG - chr8.4:15438018-15438037 MS.gene056957:intron 15.0%
!!! AAATGTTTGATTTATTATAG+TGG - chr8.4:15438372-15438391 MS.gene056957:intron 15.0%
!! AAAAAGGTAAAACAAATTCA+GGG - chr8.4:15437676-15437695 MS.gene056957:intron 20.0%
!! ACATATAAAAGAAAAAAAGG+TGG + chr8.4:15437132-15437151 None:intergenic 20.0%
!! GCAAAAGAAAACATATAATA+AGG + chr8.4:15437935-15437954 None:intergenic 20.0%
!!! ACACAATAGTTTTTTCAAAT+GGG - chr8.4:15438160-15438179 MS.gene056957:CDS 20.0%
!!! GTTATCATTAATTTCTTTTG+TGG + chr8.4:15437489-15437508 None:intergenic 20.0%
! AATACCACTAGTATCAATTA+TGG - chr8.4:15438076-15438095 MS.gene056957:intron 25.0%
! ATAATTGATACTAGTGGTAT+TGG + chr8.4:15438077-15438096 None:intergenic 25.0%
! GAAAAAGGTAAAACAAATTC+AGG - chr8.4:15437675-15437694 MS.gene056957:intron 25.0%
! TAATTGATACTAGTGGTATT+GGG + chr8.4:15438076-15438095 None:intergenic 25.0%
!! ACTTTGTATTTTACTTTGCT+TGG - chr8.4:15438588-15438607 MS.gene056957:CDS 25.0%
!! TTTCTTTGAGAATGGTAAAA+AGG + chr8.4:15437563-15437582 None:intergenic 25.0%
!!! GACACAATAGTTTTTTCAAA+TGG - chr8.4:15438159-15438178 MS.gene056957:CDS 25.0%
!!! GTTATTTCTTTCTTTGAGAA+TGG + chr8.4:15437571-15437590 None:intergenic 25.0%
ACAATTCAAACCAAACTCAT+AGG - chr8.4:15437762-15437781 MS.gene056957:intron 30.0%
ATGTATGTGATAAGGCAAAT+TGG - chr8.4:15437402-15437421 MS.gene056957:intron 30.0%
ATTAGCATCATCAGAAACAT+TGG - chr8.4:15437361-15437380 MS.gene056957:intron 30.0%
TAATTCTCTCTCTCAGTTTA+AGG - chr8.4:15438470-15438489 MS.gene056957:intron 30.0%
TCCAAATGATTTCAGTAACA+TGG + chr8.4:15436977-15436996 None:intergenic 30.0%
TCCATGTTACTGAAATCATT+TGG - chr8.4:15436973-15436992 MS.gene056957:CDS 30.0%
TGTCAGATAAGATTTGAACT+CGG + chr8.4:15437191-15437210 None:intergenic 30.0%
TTAGTCCCTAAACATAGAAA+TGG - chr8.4:15437532-15437551 MS.gene056957:CDS 30.0%
TTGTTCCTAATTCACTTGTT+TGG + chr8.4:15437451-15437470 None:intergenic 30.0%
! AAGTTGATCATCAAGGAAAA+GGG - chr8.4:15437703-15437722 MS.gene056957:intron 30.0%
!! GAAGAGCATTAACAAATCTA+CGG + chr8.4:15437821-15437840 None:intergenic 30.0%
!! GGTATGAATTCTTCTAGAAT+TGG + chr8.4:15437332-15437351 None:intergenic 30.0%
!!! AGTAGTTTTAGGAGGAATAT+GGG - chr8.4:15438732-15438751 MS.gene056957:intron 30.0%
!!! ATGGCTTTTAACTTCATCAT+TGG + chr8.4:15438443-15438462 None:intergenic 30.0%
!!! TAGTAGTTTTAGGAGGAATA+TGG - chr8.4:15438731-15438750 MS.gene056957:intron 30.0%
AACTCATAGGAAAAGCAAGA+AGG - chr8.4:15437775-15437794 MS.gene056957:intron 35.0%
AGAAATAACTCCTCTTGTCA+AGG - chr8.4:15437582-15437601 MS.gene056957:intron 35.0%
AGAGCCATAATTGATACTAG+TGG + chr8.4:15438083-15438102 None:intergenic 35.0%
AGCTTCTACTCAAAAAGAAG+AGG - chr8.4:15437629-15437648 MS.gene056957:intron 35.0%
AGTTCGTTATAAGTTGTCAG+AGG + chr8.4:15438571-15438590 None:intergenic 35.0%
CTCCACCATTTCTATGTTTA+GGG + chr8.4:15437540-15437559 None:intergenic 35.0%
GTTAATGCTCTTCAAATGCT+TGG - chr8.4:15437828-15437847 MS.gene056957:intron 35.0%
TATCGAAAATGGAAAGACGT+CGG - chr8.4:15438997-15439016 MS.gene056957:CDS 35.0%
! ATCAACTCCCTTATCTGTTT+TGG + chr8.4:15438403-15438422 None:intergenic 35.0%
! GAAGTTGATCATCAAGGAAA+AGG - chr8.4:15437702-15437721 MS.gene056957:intron 35.0%
!! TCTTGCTTTTCCTATGAGTT+TGG + chr8.4:15437775-15437794 None:intergenic 35.0%
AATGCTCTTCAAATGCTTGG+TGG - chr8.4:15437831-15437850 MS.gene056957:intron 40.0%
AGAGTGAGATGATGAGAAGT+AGG + chr8.4:15438820-15438839 None:intergenic 40.0%
AGTAACATGGAGTAGCTATG+TGG + chr8.4:15436964-15436983 None:intergenic 40.0%
CAAGCGTTTAGATCGAATCA+AGG - chr8.4:15437299-15437318 MS.gene056957:intron 40.0%
CAGTACAGCAACAAACAACA+AGG + chr8.4:15438533-15438552 None:intergenic 40.0%
GAAGAGGATAAGAAACATGC+TGG - chr8.4:15437645-15437664 MS.gene056957:intron 40.0%
GATGATGCTAATTGCTTCAC+TGG + chr8.4:15437353-15437372 None:intergenic 40.0%
GCTCCACCATTTCTATGTTT+AGG + chr8.4:15437541-15437560 None:intergenic 40.0%
GGATGACAAGTGCACAATTA+TGG - chr8.4:15437423-15437442 MS.gene056957:intron 40.0%
GGCAACACCAAAACAGATAA+GGG - chr8.4:15438393-15438412 MS.gene056957:intron 40.0%
GGGCTTATTCATTGTGACTT+AGG - chr8.4:15438180-15438199 MS.gene056957:CDS 40.0%
GTCCCTAAACATAGAAATGG+TGG - chr8.4:15437535-15437554 MS.gene056957:CDS 40.0%
TGAAGTTAAAAGCCATCTGC+AGG - chr8.4:15438447-15438466 MS.gene056957:intron 40.0%
TGACTTAGGTAGTACTAGCA+AGG - chr8.4:15438194-15438213 MS.gene056957:CDS 40.0%
TGCATGCAACTTCTCACAAA+CGG - chr8.4:15437093-15437112 MS.gene056957:CDS 40.0%
TGGAGCCAAACAAGTGAATT+AGG - chr8.4:15437443-15437462 MS.gene056957:intron 40.0%
TGGCAACACCAAAACAGATA+AGG - chr8.4:15438392-15438411 MS.gene056957:intron 40.0%
TTCACATCTCTGCTTGATTC+TGG + chr8.4:15438232-15438251 None:intergenic 40.0%
! GGGAATGAAGTTGATCATCA+AGG - chr8.4:15437696-15437715 MS.gene056957:intron 40.0%
! TAGGAAAAGCAAGAAGGTGT+TGG - chr8.4:15437781-15437800 MS.gene056957:intron 40.0%
!! ACAGATAAGGGAGTTGATGA+AGG - chr8.4:15438405-15438424 MS.gene056957:intron 40.0%
!! CAGTAGTGTAGTACTGATCT+TGG + chr8.4:15438867-15438886 None:intergenic 40.0%
AAAACTACTAGCTGTGCTGC+TGG + chr8.4:15438721-15438740 None:intergenic 45.0%
AACTCCTCTTGTCAAGGACA+AGG - chr8.4:15437588-15437607 MS.gene056957:intron 45.0%
AAGACCTTGTCCTTGACAAG+AGG + chr8.4:15437595-15437614 None:intergenic 45.0%
ACAAATCTACGGTGCAAGTC+TGG + chr8.4:15437810-15437829 None:intergenic 45.0%
ATGACCACCATAGAGAGTAG+GGG + chr8.4:15438797-15438816 None:intergenic 45.0%
ATGGCTTCACCATCTGAACT+AGG - chr8.4:15436925-15436944 MS.gene056957:CDS 45.0%
CAATCCAAGCCTAGTTCAGA+TGG + chr8.4:15436937-15436956 None:intergenic 45.0%
CATGCTGGAGAGGTAGAAAA+AGG - chr8.4:15437660-15437679 MS.gene056957:intron 45.0%
CCAAGTCCAAGTGCACAAAA+TGG - chr8.4:15438691-15438710 MS.gene056957:intron 45.0%
CTGCAGTTGCATATTCTGGT+GGG + chr8.4:15438759-15438778 None:intergenic 45.0%
GAGAGAGAATTACCTGCAGA+TGG + chr8.4:15438462-15438481 None:intergenic 45.0%
GATGACCACCATAGAGAGTA+GGG + chr8.4:15438798-15438817 None:intergenic 45.0%
GGATAAGAAACATGCTGGAG+AGG - chr8.4:15437650-15437669 MS.gene056957:intron 45.0%
GTAGAAGCTAGTGCTAGTTC+AGG + chr8.4:15437618-15437637 None:intergenic 45.0%
TAGACATGGTGCATGTGATG+AGG + chr8.4:15438919-15438938 None:intergenic 45.0%
TGTGCTGCAGTTGCATATTC+TGG + chr8.4:15438763-15438782 None:intergenic 45.0%
TTCACCATCTGAACTAGGCT+TGG - chr8.4:15436930-15436949 MS.gene056957:CDS 45.0%
! CAAATGCTTGGTGGATCTCA+AGG - chr8.4:15437840-15437859 MS.gene056957:intron 45.0%
! CCATTTTGTGCACTTGGACT+TGG + chr8.4:15438694-15438713 None:intergenic 45.0%
! CTGAGTTGTGATGATGCTAC+TGG + chr8.4:15437732-15437751 None:intergenic 45.0%
! GCAGCACAGCTAGTAGTTTT+AGG - chr8.4:15438721-15438740 MS.gene056957:intron 45.0%
! TCGTGCACAATTTTCAGCGA+TGG - chr8.4:15437256-15437275 MS.gene056957:CDS 45.0%
!! AGGGAGTTGATGAAGGTTGA+TGG - chr8.4:15438412-15438431 MS.gene056957:intron 45.0%
!! GGGTTGGAGAGTATCGAAAA+TGG - chr8.4:15438986-15439005 MS.gene056957:CDS 45.0%
!!! GCACAGCTAGTAGTTTTAGG+AGG - chr8.4:15438724-15438743 MS.gene056957:intron 45.0%
ACATGGTGCATGTGATGAGG+TGG + chr8.4:15438916-15438935 None:intergenic 50.0%
GCTGCAGTTGCATATTCTGG+TGG + chr8.4:15438760-15438779 None:intergenic 50.0%
GGATGACCACCATAGAGAGT+AGG + chr8.4:15438799-15438818 None:intergenic 50.0%
GTGAGAAGTTGCATGCAGAG+AGG + chr8.4:15437090-15437109 None:intergenic 50.0%
TATGCAACTGCAGCACATGC+AGG - chr8.4:15438766-15438785 MS.gene056957:intron 50.0%
TGAACTCGGTCTCTTGAGAG+CGG + chr8.4:15437177-15437196 None:intergenic 50.0%
! GGTGCACCATTTTGTGCACT+TGG + chr8.4:15438700-15438719 None:intergenic 50.0%
!! GTGTAGTACTGATCTTGGCC+TGG + chr8.4:15438862-15438881 None:intergenic 50.0%
AACCCGTCTCCACATGTCTC+CGG + chr8.4:15438971-15438990 None:intergenic 55.0%
CAAGGTTCACCGGAGACATG+TGG - chr8.4:15438959-15438978 MS.gene056957:CDS 55.0%
CACATGTCTCCGGTGAACCT+TGG + chr8.4:15438961-15438980 None:intergenic 55.0%
CCACATTACTGCACTGCTCC+AGG - chr8.4:15438841-15438860 MS.gene056957:CDS 55.0%
CCTGGAGCAGTGCAGTAATG+TGG + chr8.4:15438844-15438863 None:intergenic 55.0%
GGAACCCCTACTCTCTATGG+TGG - chr8.4:15438790-15438809 MS.gene056957:intron 55.0%
GGAGGAACCCCTACTCTCTA+TGG - chr8.4:15438787-15438806 MS.gene056957:intron 55.0%
GTCTACAAGACCACGCCACA+CGG - chr8.4:15438934-15438953 MS.gene056957:CDS 55.0%
TCACCGGAGACATGTGGAGA+CGG - chr8.4:15438965-15438984 MS.gene056957:CDS 55.0%
TGGTGCATGTGATGAGGTGG+CGG + chr8.4:15438913-15438932 None:intergenic 55.0%
!! GTGGCGTGGTCTTGTAGACA+TGG + chr8.4:15438933-15438952 None:intergenic 55.0%
CACCGGAGACATGTGGAGAC+GGG - chr8.4:15438966-15438985 MS.gene056957:CDS 60.0%
CCACACGGACCAAGGTTCAC+CGG - chr8.4:15438949-15438968 MS.gene056957:CDS 60.0%
GCAACTGCAGCACATGCAGG+AGG - chr8.4:15438769-15438788 MS.gene056957:intron 60.0%
GGAGACATGTGGAGACGGGT+TGG - chr8.4:15438970-15438989 MS.gene056957:CDS 60.0%
AGACCACGCCACACGGACCA+AGG - chr8.4:15438941-15438960 MS.gene056957:CDS 65.0%
CCGGTGAACCTTGGTCCGTG+TGG + chr8.4:15438952-15438971 None:intergenic 65.0%
GAACCTTGGTCCGTGTGGCG+TGG + chr8.4:15438947-15438966 None:intergenic 65.0%
!! GGTGGCGGAAGTAGCTGCTG+CGG + chr8.4:15438898-15438917 None:intergenic 65.0%
GGAAGTAGCTGCTGCGGCGG+CGG + chr8.4:15438892-15438911 None:intergenic 70.0%
GGCGGAAGTAGCTGCTGCGG+CGG + chr8.4:15438895-15438914 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 15436925 15439026 15436925 ID=MS.gene056957
chr8.4 mRNA 15436925 15439026 15436925 ID=MS.gene056957.t1;Parent=MS.gene056957
chr8.4 exon 15438837 15439026 15438837 ID=MS.gene056957.t1.exon1;Parent=MS.gene056957.t1
chr8.4 CDS 15438837 15439026 15438837 ID=cds.MS.gene056957.t1;Parent=MS.gene056957.t1
chr8.4 exon 15438496 15438678 15438496 ID=MS.gene056957.t1.exon2;Parent=MS.gene056957.t1
chr8.4 CDS 15438496 15438678 15438496 ID=cds.MS.gene056957.t1;Parent=MS.gene056957.t1
chr8.4 exon 15438090 15438371 15438090 ID=MS.gene056957.t1.exon3;Parent=MS.gene056957.t1
chr8.4 CDS 15438090 15438371 15438090 ID=cds.MS.gene056957.t1;Parent=MS.gene056957.t1
chr8.4 exon 15437483 15437559 15437483 ID=MS.gene056957.t1.exon4;Parent=MS.gene056957.t1
chr8.4 CDS 15437483 15437559 15437483 ID=cds.MS.gene056957.t1;Parent=MS.gene056957.t1
chr8.4 exon 15436925 15437284 15436925 ID=MS.gene056957.t1.exon5;Parent=MS.gene056957.t1
chr8.4 CDS 15436925 15437284 15436925 ID=cds.MS.gene056957.t1;Parent=MS.gene056957.t1
Gene Sequence

>MS.gene056957

ATGGCTTCACCATCTGAACTAGGCTTGGATTGCAAGCCACATAGCTACTCCATGTTACTGAAATCATTTGGAGAACAAAGTGATCAAAGCTACAAACTTGAAGAGTTTGTTTCTCGCTTAGAAGAAGAACGTCTCAAAATTGATGCTTTCAAACGTGAGCTTCCTCTCTGCATGCAACTTCTCACAAACGGTACAACTTTCAAACCACCTTTTTTTCTTTTATATGTAACAAACAATTTAATTATTTGTCCGCTCTCAAGAGACCGAGTTCAAATCTTATCTGACATATTTGTAAAATTGTCGTTAGTGTCACTTTAATTAATAATTTTTCTCGTGCACAATTTTCAGCGATGGAAGCATCGAAGCAGCAACTTCAAGCGTTTAGATCGAATCAAGGAGCAAAACCAATTCTAGAAGAATTCATACCAGTGAAGCAATTAGCATCATCAGAAACATTGGAAAAGACAACAAATAATAATGTATGTGATAAGGCAAATTGGATGACAAGTGCACAATTATGGAGCCAAACAAGTGAATTAGGAACAAAACAACAACAAAATTCCACAAAAGAAATTAATGATAACAATAACAATATTGGATTTAAACATTAGTCCCTAAACATAGAAATGGTGGAGCCTTTTTACCATTCTCAAAGAAAGAAATAACTCCTCTTGTCAAGGACAAGGTCTTCCTGAACTAGCACTAGCTTCTACTCAAAAAGAAGAGGATAAGAAACATGCTGGAGAGGTAGAAAAAGGTAAAACAAATTCAGGGAATGAAGTTGATCATCAAGGAAAAGGGAGTCCAGTAGCATCATCACAACTCAGACAACAAGCAACAATTCAAACCAAACTCATAGGAAAAGCAAGAAGGTGTTGGTCACCAGACTTGCACCGTAGATTTGTTAATGCTCTTCAAATGCTTGGTGGATCTCAAGGTAAACAATTATGACTTACTAATTACTAGTACTAAAGATCATAATCATCACATGATAATTGCAACTTAATCCTTATTATATGTTTTCTTTTGCAATTTCATATACTATAAAAAAGAATAGATGGTACTATATTTAATGTGTCTTTGTGTATAAATGTTTCTTTATAAAATAGTCTTAGGTATATGATTTGTTTAAGTTTGATTTTTAATATCCCAATACCACTAGTATCAATTATGGCTCTATTTGTAAAAAATAAATAAACAATTATAGTAATTTAAAATTATTATATATTTAAGGGACACAATAGTTTTTTCAAATGGGCTTATTCATTGTGACTTAGGTAGTACTAGCAAGGATCACATGATAACCAGAATCAAGCAGAGATGTGAAATTTAGTCAAATGTTATTAGGAAACGTTTTTTTTTTTTTTTTTTATAATTTAAATAGATAAACTAAAATTTTTAAAATATATATCATACTCATTAATTAATTCATCATTAACTTTTTTCGAAATGTTTGATTTATTATAGTGGCAACACCAAAACAGATAAGGGAGTTGATGAAGGTTGATGGTTTGACCAATGATGAAGTTAAAAGCCATCTGCAGGTAATTCTCTCTCTCAGTTTAAGGCAGACACAAAACAAAACAAAACAAAACAAAACAAACACCTTGTTGTTTGTTGCTGTACTGTACTAGTAATTACTACCTCTGACAACTTATAACGAACTTTGTATTTTACTTTGCTTGGTTTTTTATCTAAAATAAATTTAATTTTACATTATGTATTTTGTTATTATTGAACAGAAATATAGGCTACACACAAGAAGACCAAGTCCAAGTGCACAAAATGGTGCACCAGCAGCACAGCTAGTAGTTTTAGGAGGAATATGGGTCCCACCAGAATATGCAACTGCAGCACATGCAGGAGGAACCCCTACTCTCTATGGTGGTCATCCTACTTCTCATCATCTCACTCTGCCACATTACTGCACTGCTCCAGGCCAAGATCAGTACTACACTACTGCACCGCCGCCGCAGCAGCTACTTCCGCCACCTCATCACATGCACCATGTCTACAAGACCACGCCACACGGACCAAGGTTCACCGGAGACATGTGGAGACGGGTTGGAGAGTATCGAAAATGGAAAGACGTCGGAGAGTAG
Protein sequence

>MS.gene056957.t1

MASPSELGLDCKPHSYSMLLKSFGEQSDQSYKLEEFVSRLEEERLKIDAFKRELPLCMQLLTNAMEASKQQLQAFRSNQGAKPILEEFIPVKQLASSETLEKTTNNNVCDKANWMTSAQLWSQTKRNNSSCQGQGLPELALASTQKEEDKKHAGEVEKGKTNSGNEVDHQGKGSPVASSQLRQQATIQTKLIGKARRCWSPDLHRRFVNALQMLGGSQVATPKQIRELMKVDGLTNDEVKSHLQKYRLHTRRPSPSAQNGAPAAQLVVLGGIWVPPEYATAAHAGGTPTLYGGHPTSHHLTLPHYCTAPGQDQYYTTAPPPQQLLPPPHHMHHVYKTTPHGPRFTGDMWRRVGEYRKWKDVGE