Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056970.t1 | XP_024625823.1 | 95.7 | 469 | 16 | 1 | 7 | 471 | 90 | 558 | 3.10E-250 | 874 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056970.t1 | A4QP31 | 23.9 | 461 | 310 | 10 | 19 | 465 | 112 | 545 | 1.4e-28 | 129.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056970.t1 | A2Q4U5 | 95.7 | 469 | 16 | 1 | 7 | 471 | 90 | 558 | 2.2e-250 | 874.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056969 | MS.gene056970 | 0.813828 | 2.10E-51 | -1.69E-46 |
| MS.gene056970 | MS.gene056971 | 0.875504 | 2.93E-68 | -1.69E-46 |
| MS.gene056970 | MS.gene072381 | 0.821975 | 3.03E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 124 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTTGTTGAGACCTTAAC+TGG | 0.229867 | 7.3:+61674202 | None:intergenic |
| CTATTTCAATATGGTGATAA+AGG | 0.251197 | 7.3:-61674443 | MS.gene056970:CDS |
| CTGGGAGTAAAAGGTATTAT+AGG | 0.263890 | 7.3:+61670030 | None:intergenic |
| TATGGTTATCACCGACAATA+AGG | 0.276184 | 7.3:+61670713 | None:intergenic |
| CCGTGGCAGAAGCCTCTTAT+AGG | 0.284218 | 7.3:+61676207 | None:intergenic |
| CACATTACCAGAGGGTTATA+TGG | 0.285453 | 7.3:-61669659 | MS.gene056970:CDS |
| TTGAGATACGCCACCATCTA+TGG | 0.292789 | 7.3:-61672508 | MS.gene056970:CDS |
| GTCCGCTCTCTCGCCGCTTC+AGG | 0.294438 | 7.3:-61676119 | MS.gene056970:intron |
| TTCCTCCAACTGGGAGTAAA+AGG | 0.294649 | 7.3:+61670021 | None:intergenic |
| CTCTCGCTCGGTTCTTCCTT+CGG | 0.300080 | 7.3:-61676293 | MS.gene056970:CDS |
| CGGACTTCTTCAAAAGAAAA+TGG | 0.305727 | 7.3:-61674277 | MS.gene056970:CDS |
| TTAAGCTCACCTGCAACTTA+TGG | 0.307873 | 7.3:+61670695 | None:intergenic |
| CATTCATATGTTCCTCCAAC+TGG | 0.307892 | 7.3:+61670011 | None:intergenic |
| GACTCTCCTTCAAGCCAGTT+TGG | 0.313884 | 7.3:+61669709 | None:intergenic |
| TCCTGACATGCCTGCCGTTC+CGG | 0.318484 | 7.3:-61674368 | MS.gene056970:CDS |
| TATTTCAAAGACTGGTTATA+TGG | 0.323652 | 7.3:-61669431 | MS.gene056970:intron |
| CAAAGGTGCTAAGATGTAAA+TGG | 0.331610 | 7.3:+61672528 | None:intergenic |
| AAGCATGCATCTGACATTAA+AGG | 0.372985 | 7.3:-61674133 | MS.gene056970:CDS |
| TTTATGTTCAATCCTATAAG+AGG | 0.384999 | 7.3:-61676219 | MS.gene056970:CDS |
| ACCGGAACGGCAGGCATGTC+AGG | 0.385427 | 7.3:+61674367 | None:intergenic |
| CACCGACAATAAGGGGTTGC+TGG | 0.390794 | 7.3:+61670722 | None:intergenic |
| CATTAAAGGTTCCTCTGTTA+AGG | 0.396917 | 7.3:-61674119 | MS.gene056970:intron |
| CGGTGATAACCATAAGTTGC+AGG | 0.404355 | 7.3:-61670704 | MS.gene056970:intron |
| ATGGTTATCACCGACAATAA+GGG | 0.404357 | 7.3:+61670714 | None:intergenic |
| ACAGAACATAGCTTCTGCTT+TGG | 0.407772 | 7.3:-61674314 | MS.gene056970:CDS |
| TGGGAGTAAAAGGTATTATA+GGG | 0.411025 | 7.3:+61670031 | None:intergenic |
| TAACCCTCTGGTAATGTGAA+TGG | 0.411553 | 7.3:+61669664 | None:intergenic |
| TCCTGATCAGAGACACTAAC+TGG | 0.416501 | 7.3:+61674253 | None:intergenic |
| CGTTTACGACTTTCTTCTAA+AGG | 0.419745 | 7.3:+61669479 | None:intergenic |
| TCAACCATTCCCAATGCTAT+AGG | 0.420508 | 7.3:+61669950 | None:intergenic |
| CCGCCGCTGTAGCGGATGTC+TGG | 0.420542 | 7.3:-61676171 | MS.gene056970:CDS |
| TTCATGTGTATTTCAAAGAC+TGG | 0.426322 | 7.3:-61669439 | MS.gene056970:CDS |
| TGCATCCACTGTCCCAGTTA+AGG | 0.428581 | 7.3:-61674215 | MS.gene056970:CDS |
| GAGGAACATATGAATGAAGT+TGG | 0.444069 | 7.3:-61670004 | MS.gene056970:CDS |
| AGATGTAAATGGCAGATTCA+TGG | 0.447744 | 7.3:+61672539 | None:intergenic |
| CAGATTCCAAACTGGCTTGA+AGG | 0.448014 | 7.3:-61669715 | MS.gene056970:CDS |
| ACAAAATTAGTGAACTGGTC+AGG | 0.449084 | 7.3:+61669857 | None:intergenic |
| TGGGTTGTCTTTAGTAGAAC+CGG | 0.450172 | 7.3:+61669684 | None:intergenic |
| ACAATAATCATTGGAGCAGT+TGG | 0.454219 | 7.3:-61672472 | MS.gene056970:CDS |
| ATACCACAAAATTAGTGAAC+TGG | 0.459765 | 7.3:+61669852 | None:intergenic |
| TTCTGCCCTCAGTGAATCGT+TGG | 0.460738 | 7.3:-61669979 | MS.gene056970:CDS |
| CGTCAGCTTTACTATCTACA+AGG | 0.464502 | 7.3:-61668586 | MS.gene056970:CDS |
| TCCAGTTAGTGTCTCTGATC+AGG | 0.468213 | 7.3:-61674254 | MS.gene056970:CDS |
| CACATCAACACTATCAGATT+CGG | 0.471812 | 7.3:+61676261 | None:intergenic |
| TTGTTGTGCAGATTCCAAAC+TGG | 0.473183 | 7.3:-61669723 | MS.gene056970:intron |
| TCAGATGCCACAATAATCAT+TGG | 0.478680 | 7.3:-61672481 | MS.gene056970:CDS |
| AGCTGAGTCTGCTGCTAGGG+TGG | 0.481981 | 7.3:-61669882 | MS.gene056970:CDS |
| GTATTTGTATTGCTAACTGC+CGG | 0.482981 | 7.3:-61670771 | MS.gene056970:CDS |
| CCATCAAAAGCGGAAGTAGA+AGG | 0.484578 | 7.3:+61676148 | None:intergenic |
| GCTTCTGCCACGGACGCATA+AGG | 0.486155 | 7.3:-61676197 | MS.gene056970:CDS |
| GTCTTATCTGCAAAAGCACT+TGG | 0.489451 | 7.3:-61675283 | MS.gene056970:CDS |
| GAAGCGGGTACCGGAACGGC+AGG | 0.491040 | 7.3:+61674358 | None:intergenic |
| TGGGATGAACCTATAGCATT+GGG | 0.493761 | 7.3:-61669959 | MS.gene056970:CDS |
| CAGTCATGAACTTGGGAAGC+GGG | 0.497245 | 7.3:+61674343 | None:intergenic |
| TTGGGATGAACCTATAGCAT+TGG | 0.501174 | 7.3:-61669960 | MS.gene056970:CDS |
| GTAGAAGCCAGTCATGAACT+TGG | 0.501825 | 7.3:+61674335 | None:intergenic |
| AGGGGTGCAAGTCTCATTGA+AGG | 0.505627 | 7.3:-61674178 | MS.gene056970:CDS |
| TTTCTATTGTGACAGCCTCT+GGG | 0.509455 | 7.3:-61668615 | MS.gene056970:intron |
| TCAAGCTGAGTCTGCTGCTA+GGG | 0.510336 | 7.3:-61669885 | MS.gene056970:CDS |
| TCTAGCAGAGCCTGTGGAGG+GGG | 0.512835 | 7.3:-61675244 | MS.gene056970:CDS |
| CTGGATGAGATAGTGAATCA+TGG | 0.514028 | 7.3:+61669924 | None:intergenic |
| TAAGCGGTTTACGCCGTCGC+AGG | 0.517778 | 7.3:-61675328 | MS.gene056970:CDS |
| CTCAAGCTGAGTCTGCTGCT+AGG | 0.518463 | 7.3:-61669886 | MS.gene056970:CDS |
| TTAAGGTCTCAACAAGTGCT+AGG | 0.520505 | 7.3:-61674198 | MS.gene056970:CDS |
| CCGCTTCCCAAGTTCATGAC+TGG | 0.523437 | 7.3:-61674342 | MS.gene056970:CDS |
| GGCATCTGAACATCCATAGA+TGG | 0.528316 | 7.3:+61672495 | None:intergenic |
| TCTGCCCTCAGTGAATCGTT+GGG | 0.529878 | 7.3:-61669978 | MS.gene056970:CDS |
| TGAACTTGGGAAGCGGGTAC+CGG | 0.533221 | 7.3:+61674349 | None:intergenic |
| TAAGGTCTCAACAAGTGCTA+GGG | 0.534289 | 7.3:-61674197 | MS.gene056970:CDS |
| AACCCTCTGGTAATGTGAAT+GGG | 0.537844 | 7.3:+61669665 | None:intergenic |
| CTTCTAGCAGAGCCTGTGGA+GGG | 0.538684 | 7.3:-61675246 | MS.gene056970:CDS |
| TGGATGAGATAGTGAATCAT+GGG | 0.540784 | 7.3:+61669925 | None:intergenic |
| GAATTCACATTCACCTGAAG+CGG | 0.542370 | 7.3:+61676106 | None:intergenic |
| AACCAGCAACCCCTTATTGT+CGG | 0.554262 | 7.3:-61670724 | MS.gene056970:CDS |
| CAAAATTAGTGAACTGGTCA+GGG | 0.554549 | 7.3:+61669858 | None:intergenic |
| TATGGTGATAAAGGATCAGA+GGG | 0.557073 | 7.3:-61674434 | MS.gene056970:CDS |
| TGTTGTAATTGCAGCTTGTG+AGG | 0.557301 | 7.3:-61675359 | MS.gene056970:intron |
| TGAGATGCCATATAACCCTC+TGG | 0.558201 | 7.3:+61669652 | None:intergenic |
| CCAGTCATGAACTTGGGAAG+CGG | 0.565687 | 7.3:+61674342 | None:intergenic |
| ATTCATATGTTCCTCCAACT+GGG | 0.565926 | 7.3:+61670012 | None:intergenic |
| TCTTCTAGCAGAGCCTGTGG+AGG | 0.570334 | 7.3:-61675247 | MS.gene056970:CDS |
| TGGTTATCACCGACAATAAG+GGG | 0.578982 | 7.3:+61670715 | None:intergenic |
| AACTGCTCCAATGATTATTG+TGG | 0.579931 | 7.3:+61672474 | None:intergenic |
| CACCTGAAGCGGCGAGAGAG+CGG | 0.582553 | 7.3:+61676117 | None:intergenic |
| ATGGATAGATTCGAGCACCT+TGG | 0.584256 | 7.3:-61674470 | MS.gene056970:intron |
| CCAGACATCCGCTACAGCGG+CGG | 0.585319 | 7.3:+61676171 | None:intergenic |
| TATTTGTATTGCTAACTGCC+GGG | 0.585739 | 7.3:-61670770 | MS.gene056970:CDS |
| TCGTTCTCAATACTCTCGCT+CGG | 0.587235 | 7.3:-61676305 | MS.gene056970:CDS |
| AATCATTGGAGCAGTTGGAA+AGG | 0.588974 | 7.3:-61672467 | MS.gene056970:intron |
| GTCTCTTCTAGCAGAGCCTG+TGG | 0.591303 | 7.3:-61675250 | MS.gene056970:CDS |
| CCTATAAGAGGCTTCTGCCA+CGG | 0.592535 | 7.3:-61676207 | MS.gene056970:CDS |
| TTCTAGCAGAGCCTGTGGAG+GGG | 0.593663 | 7.3:-61675245 | MS.gene056970:CDS |
| TAAGGATTCCGCCGCTGTAG+CGG | 0.600747 | 7.3:-61676179 | MS.gene056970:CDS |
| AGGCCAGACATCCGCTACAG+CGG | 0.606545 | 7.3:+61676168 | None:intergenic |
| GCGGAATCCTTATGCGTCCG+TGG | 0.607234 | 7.3:+61676190 | None:intergenic |
| GATTCACTATCTCATCCAGC+GGG | 0.608700 | 7.3:-61669920 | MS.gene056970:CDS |
| TAGAAGCCAGTCATGAACTT+GGG | 0.608803 | 7.3:+61674336 | None:intergenic |
| TGAACCTATAGCATTGGGAA+TGG | 0.609871 | 7.3:-61669954 | MS.gene056970:CDS |
| TTGTGAGGAGCAACAGTAAG+CGG | 0.610899 | 7.3:-61675344 | MS.gene056970:CDS |
| CTTTGGAATATATTGCTGAA+CGG | 0.614328 | 7.3:-61674297 | MS.gene056970:CDS |
| TAGATGGTGGCGTATCTCAA+AGG | 0.614821 | 7.3:+61672511 | None:intergenic |
| TTGGGAAGCGGGTACCGGAA+CGG | 0.614909 | 7.3:+61674354 | None:intergenic |
| ATATGGTGATAAAGGATCAG+AGG | 0.617898 | 7.3:-61674435 | MS.gene056970:CDS |
| TGAGCATCAGAGACACCCGC+TGG | 0.621306 | 7.3:+61669905 | None:intergenic |
| CACTTGTTGAGACCTTAACT+GGG | 0.623226 | 7.3:+61674203 | None:intergenic |
| TGGCTTGAAGGAGAGTCCAC+CGG | 0.633134 | 7.3:-61669703 | MS.gene056970:CDS |
| GGGGTGCAAGTCTCATTGAA+GGG | 0.639854 | 7.3:-61674177 | MS.gene056970:CDS |
| ATCTGAACATCCATAGATGG+TGG | 0.640921 | 7.3:+61672498 | None:intergenic |
| AACCCATTCACATTACCAGA+GGG | 0.646195 | 7.3:-61669667 | MS.gene056970:CDS |
| TGAGACCTTAACTGGGACAG+TGG | 0.646520 | 7.3:+61674210 | None:intergenic |
| TCATCCCAACGATTCACTGA+GGG | 0.651225 | 7.3:+61669974 | None:intergenic |
| GATTCGGAATCAGACTCCGA+AGG | 0.656584 | 7.3:+61676277 | None:intergenic |
| GCTTCTTCATCAGCCTGCGA+CGG | 0.661506 | 7.3:+61675315 | None:intergenic |
| GTTGTCTTTAGTAGAACCGG+TGG | 0.662347 | 7.3:+61669687 | None:intergenic |
| AAGGTCTCAACAAGTGCTAG+GGG | 0.664529 | 7.3:-61674196 | MS.gene056970:CDS |
| CAACCCATTCACATTACCAG+AGG | 0.670122 | 7.3:-61669668 | MS.gene056970:CDS |
| TGATTCACTATCTCATCCAG+CGG | 0.671487 | 7.3:-61669921 | MS.gene056970:CDS |
| CATATTGAAATAGAAAACCA+AGG | 0.685998 | 7.3:+61674453 | None:intergenic |
| GGACAAGTATCCATCAAAAG+CGG | 0.692177 | 7.3:+61676138 | None:intergenic |
| TAAGGGACATACCTTAACAG+AGG | 0.699164 | 7.3:+61674108 | None:intergenic |
| GGATGAGATAGTGAATCATG+GGG | 0.716827 | 7.3:+61669926 | None:intergenic |
| TTCATCCCAACGATTCACTG+AGG | 0.728891 | 7.3:+61669973 | None:intergenic |
| AAGCTGACGAATGTTCCCAG+AGG | 0.775862 | 7.3:+61668600 | None:intergenic |
| ATGGTGATAAAGGATCAGAG+GGG | 0.839783 | 7.3:-61674433 | MS.gene056970:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 61668579 | 61676328 | 61668579 | ID=MS.gene056970 |
| chr7.3 | mRNA | 61668579 | 61676328 | 61668579 | ID=MS.gene056970.t1;Parent=MS.gene056970 |
| chr7.3 | exon | 61676120 | 61676328 | 61676120 | ID=MS.gene056970.t1.exon1;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61676120 | 61676328 | 61676120 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61675223 | 61675367 | 61675223 | ID=MS.gene056970.t1.exon2;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61675223 | 61675367 | 61675223 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61674120 | 61674484 | 61674120 | ID=MS.gene056970.t1.exon3;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61674120 | 61674484 | 61674120 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61672468 | 61672572 | 61672468 | ID=MS.gene056970.t1.exon4;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61672468 | 61672572 | 61672468 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61670705 | 61670845 | 61670705 | ID=MS.gene056970.t1.exon5;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61670705 | 61670845 | 61670705 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61669856 | 61670059 | 61669856 | ID=MS.gene056970.t1.exon6;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61669856 | 61670059 | 61669856 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61669642 | 61669734 | 61669642 | ID=MS.gene056970.t1.exon7;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61669642 | 61669734 | 61669642 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61669432 | 61669543 | 61669432 | ID=MS.gene056970.t1.exon8;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61669432 | 61669543 | 61669432 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| chr7.3 | exon | 61668579 | 61668622 | 61668579 | ID=MS.gene056970.t1.exon9;Parent=MS.gene056970.t1 |
| chr7.3 | CDS | 61668579 | 61668622 | 61668579 | ID=cds.MS.gene056970.t1;Parent=MS.gene056970.t1 |
| Gene Sequence |
| Protein sequence |
