AlfalfaGEDB | MS.gene056999

M. sativa cultivar XinJiangDaYe / MS.gene056999

KEGG: -
GO: -
PFAM: PF12023.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056999.t1	XP_024632952.1	93.5	108	6	1	1	107	1	108	6.40E-50	206.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056999.t1	G7IS34	93.5	108	6	1	1	107	1	108	4.6e-50	206.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051962	MS.gene056999	0.931949	1.77E-94	-1.69E-46
MS.gene056999	MS.gene073454	0.814626	1.40E-51	-1.69E-46
MS.gene056999	MS.gene85234	0.899254	2.38E-77	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056999.t1	MTR_2g099630	93.519	108	6	1	1	107	1	108	1.30e-69	203
MS.gene056999.t1	MTR_8g018320	65.049	103	33	2	1	101	1	102	2.06e-40	129
MS.gene056999.t1	MTR_8g018360	65.049	103	33	2	1	101	1	102	3.77e-39	129
MS.gene056999.t1	MTR_4g125990	49.180	122	44	4	2	106	3	123	1.10e-30	105
MS.gene056999.t1	MTR_3g104640	46.667	105	54	2	4	106	6	110	9.23e-22	82.8
MS.gene056999.t1	MTR_1g015810	60.417	48	19	0	60	107	72	119	1.10e-15	67.4
MS.gene056999.t1	MTR_7g109290	54.902	51	22	1	56	106	42	91	3.38e-12	57.8
MS.gene056999.t1	MTR_1g087730	47.368	57	28	2	51	106	51	106	2.78e-11	55.8
MS.gene056999.t1	MTR_1g098310	50.000	48	24	0	58	105	38	85	4.07e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056999.t1	AT5G11970	56.098	82	33	2	26	107	27	105	4.56e-24	88.6
MS.gene056999.t1	AT1G72720	34.921	126	61	4	3	107	2	127	4.44e-16	68.9
MS.gene056999.t1	AT2G19460	47.297	74	38	1	24	97	37	109	2.13e-15	66.6
MS.gene056999.t1	AT2G19460	47.297	74	38	1	24	97	89	161	6.22e-15	66.6
MS.gene056999.t1	AT3G13910	53.333	60	20	3	45	97	39	97	3.79e-13	60.5
MS.gene056999.t1	AT3G13910	53.333	60	20	3	45	97	39	97	5.52e-13	60.5
MS.gene056999.t1	AT3G05725	56.818	44	19	0	62	105	80	123	1.60e-11	57.0

Find 27 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTTCTTCCTCTGCAATTC+AGG	0.144268	2.1:+4977990	None:intergenic
TCAGTTCTTGCATGCTAGTT+GGG	0.283001	2.1:+4978120	None:intergenic
CTCAGTTCTTGCATGCTAGT+TGG	0.365308	2.1:+4978119	None:intergenic
ATGAAGCTCCAACTCTTTGA+TGG	0.366122	2.1:+4978020	None:intergenic
TCTTTATTACATGCCAGGTT+TGG	0.397283	2.1:+4978068	None:intergenic
CAGAGGAAGAAGAGAATTGC+TGG	0.419243	2.1:-4977981	MS.gene056999:CDS
GATGAAAAGCTACAAATTGA+AGG	0.461024	2.1:-4978152	MS.gene056999:CDS
ACTTACACTCAAGCTATGTA+TGG	0.473577	2.1:-4977888	MS.gene056999:CDS
TCACCTCTTTATTACATGCC+AGG	0.477597	2.1:+4978063	None:intergenic
AAAGAGGTGAAAATGAAGAA+AGG	0.496185	2.1:-4978050	MS.gene056999:CDS
GGTCTCTAAGAAAGAGTCTC+AGG	0.496771	2.1:-4977923	MS.gene056999:CDS
AAGAGGTGAAAATGAAGAAA+GGG	0.497586	2.1:-4978049	MS.gene056999:CDS
AAAGCTACAAATTGAAGGTA+AGG	0.497739	2.1:-4978147	MS.gene056999:CDS
TGAAGCTCCAACTCTTTGAT+GGG	0.510832	2.1:+4978021	None:intergenic
CAGTTCTTGCATGCTAGTTG+GGG	0.522810	2.1:+4978121	None:intergenic
GGAAAATCCCATCAAAGAGT+TGG	0.532658	2.1:-4978028	MS.gene056999:CDS
GTGCTGAAGGGAAAATGAAA+GGG	0.551730	2.1:-4977944	MS.gene056999:CDS
AAACCTGGCATGTAATAAAG+AGG	0.555221	2.1:-4978066	MS.gene056999:CDS
TACAAAATGTATGGTGCTGA+AGG	0.562238	2.1:-4977957	MS.gene056999:CDS
ATTGCTGGCTACAAAATGTA+TGG	0.571795	2.1:-4977966	MS.gene056999:CDS
ACACTCAAGCTATGTATGGA+TGG	0.580620	2.1:-4977884	MS.gene056999:CDS
GGTGCTGAAGGGAAAATGAA+AGG	0.580805	2.1:-4977945	MS.gene056999:CDS
TTATGTTTATAATCCAAACC+TGG	0.587054	2.1:-4978081	MS.gene056999:CDS
CTCTAAGAAAGAGTCTCAGG+TGG	0.614706	2.1:-4977920	MS.gene056999:CDS
ACAAAATGTATGGTGCTGAA+GGG	0.616006	2.1:-4977956	MS.gene056999:CDS
TGAATGATCCTGAATTGCAG+AGG	0.659598	2.1:-4977998	MS.gene056999:CDS
CTCAAGCTATGTATGGATGG+TGG	0.724201	2.1:-4977881	MS.gene056999:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTATGTTTATAATCCAAACC+TGG	-	chr2.1:4977976-4977995	MS.gene056999:CDS	25.0%
	AAAGAGGTGAAAATGAAGAA+AGG	-	chr2.1:4978007-4978026	MS.gene056999:CDS	30.0%
	AAAGCTACAAATTGAAGGTA+AGG	-	chr2.1:4977910-4977929	MS.gene056999:CDS	30.0%
	AAGAGGTGAAAATGAAGAAA+GGG	-	chr2.1:4978008-4978027	MS.gene056999:CDS	30.0%
	GATGAAAAGCTACAAATTGA+AGG	-	chr2.1:4977905-4977924	MS.gene056999:CDS	30.0%
	AAACCTGGCATGTAATAAAG+AGG	-	chr2.1:4977991-4978010	MS.gene056999:CDS	35.0%
	ACTTACACTCAAGCTATGTA+TGG	-	chr2.1:4978169-4978188	MS.gene056999:CDS	35.0%
	TCTTTATTACATGCCAGGTT+TGG	+	chr2.1:4977992-4978011	None:intergenic	35.0%
!	ACAAAATGTATGGTGCTGAA+GGG	-	chr2.1:4978101-4978120	MS.gene056999:CDS	35.0%
!	ATTGCTGGCTACAAAATGTA+TGG	-	chr2.1:4978091-4978110	MS.gene056999:CDS	35.0%
!	TACAAAATGTATGGTGCTGA+AGG	-	chr2.1:4978100-4978119	MS.gene056999:CDS	35.0%
	ACACTCAAGCTATGTATGGA+TGG	-	chr2.1:4978173-4978192	MS.gene056999:CDS	40.0%
	ATGAAGCTCCAACTCTTTGA+TGG	+	chr2.1:4978040-4978059	None:intergenic	40.0%
	GGAAAATCCCATCAAAGAGT+TGG	-	chr2.1:4978029-4978048	MS.gene056999:CDS	40.0%
	GTGCTGAAGGGAAAATGAAA+GGG	-	chr2.1:4978113-4978132	MS.gene056999:CDS	40.0%
	TCACCTCTTTATTACATGCC+AGG	+	chr2.1:4977997-4978016	None:intergenic	40.0%
	TCAGTTCTTGCATGCTAGTT+GGG	+	chr2.1:4977940-4977959	None:intergenic	40.0%
	TGAAGCTCCAACTCTTTGAT+GGG	+	chr2.1:4978039-4978058	None:intergenic	40.0%
	TGAATGATCCTGAATTGCAG+AGG	-	chr2.1:4978059-4978078	MS.gene056999:CDS	40.0%
	CAGAGGAAGAAGAGAATTGC+TGG	-	chr2.1:4978076-4978095	MS.gene056999:CDS	45.0%
	CAGTTCTTGCATGCTAGTTG+GGG	+	chr2.1:4977939-4977958	None:intergenic	45.0%
	CTCAAGCTATGTATGGATGG+TGG	-	chr2.1:4978176-4978195	MS.gene056999:CDS	45.0%
	CTCAGTTCTTGCATGCTAGT+TGG	+	chr2.1:4977941-4977960	None:intergenic	45.0%
	CTCTTCTTCCTCTGCAATTC+AGG	+	chr2.1:4978070-4978089	None:intergenic	45.0%
!	CTCTAAGAAAGAGTCTCAGG+TGG	-	chr2.1:4978137-4978156	MS.gene056999:CDS	45.0%
!	GGTCTCTAAGAAAGAGTCTC+AGG	-	chr2.1:4978134-4978153	MS.gene056999:CDS	45.0%
!	GGTGCTGAAGGGAAAATGAA+AGG	-	chr2.1:4978112-4978131	MS.gene056999:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	4977878	4978201	4977878	ID=MS.gene056999
chr2.1	mRNA	4977878	4978201	4977878	ID=MS.gene056999.t1;Parent=MS.gene056999
chr2.1	exon	4977878	4978201	4977878	ID=MS.gene056999.t1.exon1;Parent=MS.gene056999.t1
chr2.1	CDS	4977878	4978201	4977878	ID=cds.MS.gene056999.t1;Parent=MS.gene056999.t1

Gene Sequence	>MS.gene056999 ATGGAATTCAGATCAAAGTCATGCAGAGATGAAAAGCTACAAATTGAAGGTAAGGTGACCCCAACTAGCATGCAAGAACTGAGATGTTACAGTGCTAATTATGTTTATAATCCAAACCTGGCATGTAATAAAGAGGTGAAAATGAAGAAAGGGAAAATCCCATCAAAGAGTTGGAGCTTCATGAATGATCCTGAATTGCAGAGGAAGAAGAGAATTGCTGGCTACAAAATGTATGGTGCTGAAGGGAAAATGAAAGGGTCTCTAAGAAAGAGTCTCAGGTGGATAAAAAATACTTACACTCAAGCTATGTATGGATGGTGGTGA
Protein sequence	>MS.gene056999.t1 MEFRSKSCRDEKLQIEGKVTPTSMQELRCYSANYVYNPNLACNKEVKMKKGKIPSKSWSFMNDPELQRKKRIAGYKMYGAEGKMKGSLRKSLRWIKNTYTQAMYGWW