AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05725

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05725.t1 MTR_3g464570 98.400 375 6 0 11 385 4 378 0.0 780
MS.gene05725.t1 MTR_3g464570 98.175 274 5 0 112 385 1 274 0.0 563
MS.gene05725.t1 MTR_5g071350 67.500 360 107 4 36 385 42 401 0.0 532
MS.gene05725.t1 MTR_2g015720 50.992 353 153 6 31 383 26 358 3.24e-125 365
MS.gene05725.t1 MTR_4g088030 49.284 349 161 4 36 384 19 351 3.79e-120 352
MS.gene05725.t1 MTR_4g079700 49.851 335 151 4 49 383 46 363 1.61e-117 346
MS.gene05725.t1 MTR_3g049330 48.087 366 174 6 20 384 1 351 7.77e-116 341
MS.gene05725.t1 MTR_2g091175 48.657 335 155 4 50 384 50 367 1.17e-115 341
MS.gene05725.t1 MTR_4g087730 49.851 335 152 4 50 384 45 363 5.13e-115 339
MS.gene05725.t1 MTR_5g034810 46.467 368 176 9 19 384 5 353 1.81e-106 317
MS.gene05725.t1 MTR_3g021060 44.868 341 170 5 44 383 40 363 3.07e-105 315
MS.gene05725.t1 MTR_3g021090 44.807 337 168 6 44 379 25 344 4.90e-105 316
MS.gene05725.t1 MTR_3g035830 43.695 341 174 5 44 383 25 348 1.91e-104 312
MS.gene05725.t1 MTR_2g073510 45.970 335 166 5 50 383 33 353 3.05e-103 309
MS.gene05725.t1 MTR_1g112210 41.124 338 181 5 50 383 59 382 1.41e-97 295
MS.gene05725.t1 MTR_4g121560 39.943 353 186 6 48 384 112 454 5.82e-96 294
MS.gene05725.t1 MTR_7g093450 39.247 372 202 7 28 384 226 588 6.99e-96 298
MS.gene05725.t1 MTR_5g069320 53.929 280 72 6 105 376 156 386 9.71e-96 291
MS.gene05725.t1 MTR_1g112210 42.012 338 165 7 50 383 59 369 2.07e-93 285
MS.gene05725.t1 MTR_0136s0010 37.731 379 203 9 31 384 140 510 5.43e-93 288
MS.gene05725.t1 MTR_2g073500 41.329 346 170 8 47 384 26 346 1.55e-91 279
MS.gene05725.t1 MTR_5g033800 37.356 348 197 5 50 384 145 484 6.86e-91 282
MS.gene05725.t1 MTR_4g126950 38.439 346 193 5 50 383 109 446 2.75e-90 279
MS.gene05725.t1 MTR_5g059400 38.527 353 196 6 47 385 172 517 3.88e-90 281
MS.gene05725.t1 MTR_1g116130 38.527 353 196 6 47 385 172 517 3.88e-90 281
MS.gene05725.t1 MTR_1g050448 39.583 336 188 5 50 383 53 375 1.94e-89 275
MS.gene05725.t1 MTR_8g069790 39.143 350 188 6 50 384 178 517 6.06e-89 278
MS.gene05725.t1 MTR_4g016940 38.728 346 196 6 50 383 86 427 2.63e-88 273
MS.gene05725.t1 MTR_3g070900 41.595 351 187 7 47 383 103 449 3.17e-86 269
MS.gene05725.t1 MTR_1g111010 41.618 346 168 11 43 384 27 342 7.36e-85 262
MS.gene05725.t1 MTR_3g071650 39.943 353 186 7 50 385 89 432 1.69e-84 264
MS.gene05725.t1 MTR_8g463790 38.810 353 191 7 47 384 68 410 3.02e-83 260
MS.gene05725.t1 MTR_7g109550 38.304 342 186 8 50 380 137 464 7.64e-83 261
MS.gene05725.t1 MTR_4g014440 34.711 363 217 8 39 385 51 409 2.37e-82 258
MS.gene05725.t1 MTR_7g068920 38.150 346 198 5 50 383 80 421 8.21e-79 249
MS.gene05725.t1 MTR_4g014430 36.994 346 201 6 49 383 107 446 3.54e-78 248
MS.gene05725.t1 MTR_5g082880 38.068 352 197 8 50 384 165 512 2.28e-77 248
MS.gene05725.t1 MTR_5g083260 37.500 360 199 8 43 385 76 426 4.20e-77 244
MS.gene05725.t1 MTR_2g022560 35.813 363 204 9 37 381 84 435 6.42e-77 245
MS.gene05725.t1 MTR_7g070060 34.278 353 212 8 48 384 62 410 5.88e-75 238
MS.gene05725.t1 MTR_7g094750 35.097 359 193 9 50 384 91 433 1.73e-73 236
MS.gene05725.t1 MTR_1g058380 37.006 354 193 8 49 384 54 395 6.30e-72 231
MS.gene05725.t1 MTR_3g090120 34.492 374 202 9 31 384 110 460 2.05e-70 228
MS.gene05725.t1 MTR_8g093530 34.483 348 195 6 50 384 90 417 3.73e-70 227
MS.gene05725.t1 MTR_5g033800 37.021 235 137 2 50 273 145 379 9.57e-60 198
MS.gene05725.t1 MTR_7g070060 32.476 311 190 8 90 384 2 308 1.09e-55 185
MS.gene05725.t1 MTR_7g094750 35.685 241 134 4 50 273 91 327 7.87e-53 179
MS.gene05725.t1 MTR_6g016880 30.114 352 220 9 49 383 77 419 7.80e-45 160
MS.gene05725.t1 MTR_3g109840 30.653 398 239 13 10 383 31 415 9.32e-45 160
MS.gene05725.t1 MTR_7g070060 31.197 234 152 3 48 272 62 295 4.78e-44 155
MS.gene05725.t1 MTR_7g019120 29.023 348 227 8 50 383 79 420 5.98e-43 155
MS.gene05725.t1 MTR_5g035080 32.213 357 208 10 46 379 71 416 1.73e-42 154
MS.gene05725.t1 MTR_2g016060 32.713 376 192 18 41 379 45 396 2.09e-42 153
MS.gene05725.t1 MTR_8g104970 30.790 367 224 10 34 379 104 461 6.83e-42 153
MS.gene05725.t1 MTR_5g035090 31.285 358 211 12 45 379 78 423 1.72e-41 151
MS.gene05725.t1 MTR_8g027900 29.915 351 223 10 50 383 211 555 1.22e-40 151
MS.gene05725.t1 MTR_6g016875 30.749 387 210 15 23 379 46 404 1.38e-40 149
MS.gene05725.t1 MTR_4g125060 29.839 372 193 13 49 383 170 510 1.38e-39 147
MS.gene05725.t1 MTR_7g033230 29.714 350 224 10 47 379 261 605 1.58e-39 149
MS.gene05725.t1 MTR_5g035150 29.888 358 222 11 43 379 78 427 1.78e-39 146
MS.gene05725.t1 MTR_4g125060 29.839 372 193 13 49 383 131 471 2.55e-39 146
MS.gene05725.t1 MTR_4g125060 29.839 372 193 13 49 383 199 539 3.68e-39 147
MS.gene05725.t1 MTR_7g033230 29.714 350 224 10 47 379 331 675 6.82e-39 147
MS.gene05725.t1 MTR_8g093870 30.748 361 204 15 49 383 132 472 2.86e-37 140
MS.gene05725.t1 MTR_1g076690 28.729 362 208 12 50 379 223 566 2.93e-37 142
MS.gene05725.t1 MTR_6g061710 27.056 377 224 14 41 383 155 514 4.92e-36 138
MS.gene05725.t1 MTR_8g099300 28.406 345 227 8 50 378 74 414 1.34e-34 132
MS.gene05725.t1 MTR_5g035070 28.814 354 227 12 49 383 86 433 2.41e-33 129
MS.gene05725.t1 MTR_6g007777 27.506 389 236 13 29 383 81 457 5.51e-33 129
MS.gene05725.t1 MTR_4g092830 25.843 356 223 12 50 379 48 388 4.02e-30 120
MS.gene05725.t1 MTR_7g019140 45.631 103 56 0 36 138 65 167 2.36e-28 109
MS.gene05725.t1 MTR_6g016240 25.124 402 227 14 20 383 51 416 6.62e-28 114
MS.gene05725.t1 MTR_3g021150 46.491 114 53 3 235 347 1 107 6.31e-25 98.6
MS.gene05725.t1 MTR_2g022580 36.800 125 66 3 110 221 2 126 1.28e-22 92.4
MS.gene05725.t1 MTR_5g035120 44.086 93 52 0 46 138 71 163 2.12e-21 90.5
MS.gene05725.t1 MTR_5g035140 25.714 245 154 10 160 383 28 265 1.11e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05725.t1 AT5G58600 57.841 389 147 8 11 385 17 402 1.25e-169 479
MS.gene05725.t1 AT2G30010 55.858 367 148 7 31 385 34 398 1.93e-152 436
MS.gene05725.t1 AT2G30010 55.858 367 148 7 31 385 77 441 2.56e-152 437
MS.gene05725.t1 AT5G58600 58.242 273 103 3 11 272 17 289 7.81e-123 357
MS.gene05725.t1 AT1G29050 47.425 369 176 4 17 383 26 378 1.01e-122 360
MS.gene05725.t1 AT2G42570 51.343 335 147 4 50 384 47 365 5.62e-120 352
MS.gene05725.t1 AT2G34070 49.280 347 157 5 40 383 53 383 1.88e-118 349
MS.gene05725.t1 AT2G31110 48.071 337 153 5 50 383 45 362 1.26e-112 333
MS.gene05725.t1 AT3G14850 46.588 337 167 5 44 380 28 351 6.24e-107 318
MS.gene05725.t1 AT2G30900 46.313 339 165 8 50 385 43 367 1.57e-103 310
MS.gene05725.t1 AT1G78710 43.895 344 172 9 44 384 32 357 1.58e-100 302
MS.gene05725.t1 AT3G54260 42.478 339 177 4 50 384 51 375 1.13e-97 296
MS.gene05725.t1 AT5G49340 41.525 354 181 8 48 384 98 442 5.15e-95 291
MS.gene05725.t1 AT5G06700 40.571 350 184 8 50 384 255 595 2.75e-94 294
MS.gene05725.t1 AT3G12060 40.741 351 184 8 49 384 195 536 2.20e-93 290
MS.gene05725.t1 AT5G20590 40.230 348 187 6 50 384 141 480 1.63e-92 286
MS.gene05725.t1 AT2G34070 49.811 265 116 4 120 383 1 249 9.74e-87 263
MS.gene05725.t1 AT1G60790 38.636 352 191 7 50 384 190 533 1.35e-86 272
MS.gene05725.t1 AT3G62390 36.311 347 199 6 50 385 138 473 4.46e-85 266
MS.gene05725.t1 AT3G11030 40.113 354 185 8 50 385 106 450 1.14e-84 265
MS.gene05725.t1 AT5G01360 37.752 347 199 7 50 383 36 378 2.16e-82 256
MS.gene05725.t1 AT3G55990 39.542 349 193 7 50 384 140 484 9.78e-82 258
MS.gene05725.t1 AT5G01360 37.752 347 199 7 50 383 92 434 1.07e-81 256
MS.gene05725.t1 AT5G19160 37.640 356 186 7 50 381 99 442 2.86e-79 251
MS.gene05725.t1 AT1G73140 36.752 351 197 8 49 384 66 406 4.10e-79 249
MS.gene05725.t1 AT2G40150 38.655 357 185 9 50 381 71 418 1.17e-77 246
MS.gene05725.t1 AT3G06080 36.592 358 200 7 42 381 95 443 1.15e-76 244
MS.gene05725.t1 AT2G31110 49.537 216 108 1 50 265 45 259 1.40e-76 238
MS.gene05725.t1 AT2G38320 33.671 395 231 12 15 384 17 405 2.73e-76 242
MS.gene05725.t1 AT2G40320 37.709 358 201 9 43 385 74 424 4.56e-76 242
MS.gene05725.t1 AT5G01620 34.278 353 214 6 43 383 103 449 1.08e-75 241
MS.gene05725.t1 AT5G01620 34.278 353 214 6 43 383 103 449 1.08e-75 241
MS.gene05725.t1 AT5G01620 34.278 353 214 6 43 383 111 457 1.48e-75 241
MS.gene05725.t1 AT1G48880 36.571 350 187 7 48 384 113 440 1.26e-73 236
MS.gene05725.t1 AT2G40160 32.850 414 242 12 4 385 16 425 3.54e-73 234
MS.gene05725.t1 AT2G40160 32.850 414 242 12 4 385 16 425 7.31e-73 234
MS.gene05725.t1 AT2G30900 49.545 220 108 3 50 267 43 261 1.05e-72 228
MS.gene05725.t1 AT3G14850 44.444 261 132 5 120 380 1 248 4.70e-72 226
MS.gene05725.t1 AT3G11570 37.612 335 181 7 66 384 98 420 5.52e-71 229
MS.gene05725.t1 AT2G38320 35.692 325 186 10 77 384 4 322 9.68e-68 217
MS.gene05725.t1 AT2G31110 48.444 225 94 5 162 383 9 214 9.03e-67 211
MS.gene05725.t1 AT5G06230 36.310 336 186 7 65 384 42 365 1.35e-66 216
MS.gene05725.t1 AT5G06230 36.310 336 186 7 65 384 83 406 2.78e-66 216
MS.gene05725.t1 AT5G19160 39.662 237 130 2 50 273 99 335 2.46e-59 196
MS.gene05725.t1 AT5G20590 40.506 237 128 4 50 273 141 377 4.20e-59 196
MS.gene05725.t1 AT3G62390 37.179 234 136 3 50 272 138 371 2.75e-56 190
MS.gene05725.t1 AT3G06080 34.286 245 148 3 42 273 95 339 9.79e-50 171
MS.gene05725.t1 AT2G40160 31.757 296 176 6 4 273 16 311 2.37e-49 169
MS.gene05725.t1 AT1G78710 43.889 180 94 5 44 220 25 200 1.85e-45 158
MS.gene05725.t1 AT1G78710 46.226 106 48 3 279 384 201 297 7.97e-22 95.1
MS.gene05725.t1 AT5G06230 36.199 221 128 3 65 272 83 303 1.23e-43 154
MS.gene05725.t1 AT1G01430 30.252 357 224 11 48 384 97 448 1.30e-43 157
MS.gene05725.t1 AT5G64020 29.155 367 212 15 50 383 57 408 8.82e-42 151
MS.gene05725.t1 AT2G37720 28.846 364 207 13 49 383 142 482 4.29e-41 151
MS.gene05725.t1 AT1G70230 29.167 360 210 9 46 383 75 411 8.64e-39 144
MS.gene05725.t1 AT5G51640 28.338 367 210 12 47 378 135 483 4.75e-38 143
MS.gene05725.t1 AT4G01080 28.532 361 220 10 44 382 85 429 5.97e-38 142
MS.gene05725.t1 AT4G25360 29.006 362 213 12 47 378 172 519 2.59e-37 142
MS.gene05725.t1 AT4G25360 29.006 362 213 12 47 378 172 519 2.59e-37 142
MS.gene05725.t1 AT5G15890 27.324 355 230 11 40 379 177 518 4.81e-37 140
MS.gene05725.t1 AT3G02440 28.011 357 219 12 50 383 127 468 1.45e-36 139
MS.gene05725.t1 AT3G02440 28.011 357 219 12 50 383 135 476 1.49e-36 139
MS.gene05725.t1 AT5G64470 30.105 382 205 17 39 383 42 398 2.39e-36 137
MS.gene05725.t1 AT4G23790 28.108 370 209 11 50 383 79 427 7.81e-36 136
MS.gene05725.t1 AT3G28150 29.379 354 217 10 50 383 70 410 1.98e-35 134
MS.gene05725.t1 AT5G15900 27.528 356 233 11 45 383 62 409 6.55e-35 133
MS.gene05725.t1 AT5G15900 27.528 356 233 11 45 383 81 428 6.90e-35 134
MS.gene05725.t1 AT4G11090 28.080 349 234 8 50 383 79 425 9.73e-35 133
MS.gene05725.t1 AT5G20680 27.473 364 210 11 50 383 118 457 3.21e-34 132
MS.gene05725.t1 AT5G20680 27.473 364 210 11 50 383 194 533 5.74e-34 132
MS.gene05725.t1 AT5G64470 29.211 380 205 17 39 379 42 396 5.87e-34 130
MS.gene05725.t1 AT5G20680 27.473 364 210 11 50 383 212 551 7.30e-34 132
MS.gene05725.t1 AT5G20680 27.473 364 210 11 50 383 212 551 7.30e-34 132
MS.gene05725.t1 AT5G20680 27.473 364 210 11 50 383 212 551 7.30e-34 132
MS.gene05725.t1 AT5G20680 27.473 364 210 11 50 383 212 551 7.30e-34 132
MS.gene05725.t1 AT2G14530 27.520 367 217 12 47 383 59 406 1.64e-33 129
MS.gene05725.t1 AT5G64470 27.306 271 154 11 39 279 42 299 4.10e-18 84.7
MS.gene05725.t1 AT5G64470 27.306 271 154 11 39 279 42 299 4.59e-18 84.7

Find 101 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTTGAGGATTGGTAAAA+AGG 0.207215 3.1:-48501706 None:intergenic
ACTTTCAACCTTTAGATTCT+TGG 0.221601 3.1:+48502071 MS.gene05725:CDS
GAGGAACTTGTTGAGAATAA+AGG 0.233324 3.1:-48501525 None:intergenic
AATGTTTGTTGGTGATTCTT+TGG 0.234169 3.1:+48501966 MS.gene05725:CDS
GATATCTTGTCATTTAATTC+TGG 0.260773 3.1:+48502789 MS.gene05725:CDS
TGCAGTCATTGGTGTCTTCC+TGG 0.262446 3.1:+48505034 MS.gene05725:CDS
CAATGGAGTTCAGTTTCTTA+TGG 0.266500 3.1:+48501924 MS.gene05725:CDS
TCTATGACCTTCATTTGTTC+TGG 0.285604 3.1:-48504872 None:intergenic
CATGAATCATTGGTCTTTGA+TGG 0.287494 3.1:-48501627 None:intergenic
ATGTTTGTTGGTGATTCTTT+GGG 0.290042 3.1:+48501967 MS.gene05725:CDS
TAAGTGCAGGTGGGATTATA+TGG 0.296724 3.1:+48503079 MS.gene05725:intron
CTTGTTGTTGTTAGGTTCAA+TGG 0.297456 3.1:+48501907 MS.gene05725:intron
TGACTGCAATCTGAGTAAAT+AGG 0.297908 3.1:-48505019 None:intergenic
AACAATTCATTCCAAGTATC+AGG 0.318369 3.1:-48505061 None:intergenic
ACTCCAGAAGTCCATTCATT+TGG 0.330409 3.1:-48504770 None:intergenic
TCTAGACATTACAATGTTAT+CGG 0.331996 3.1:+48504931 MS.gene05725:CDS
AAGAATTGCTATGGAGAAAC+TGG 0.335248 3.1:+48504809 MS.gene05725:CDS
CTGGAGGAGATAGATGTAAA+TGG 0.335315 3.1:+48502747 MS.gene05725:CDS
AGGTGGGATTATATGGAATT+AGG 0.335955 3.1:+48503086 MS.gene05725:intron
ACAACATGTGCATTGTTTGT+AGG 0.345050 3.1:+48501658 MS.gene05725:CDS
CTGATTACCTTAGATATAGA+TGG 0.349767 3.1:+48501779 MS.gene05725:CDS
CTCCAGAAGTCCATTCATTT+GGG 0.353051 3.1:-48504769 None:intergenic
AATGGAGTTCAGTTTCTTAT+GGG 0.363279 3.1:+48501925 MS.gene05725:CDS
GTAATGTCTAGAAGATAAAC+AGG 0.364992 3.1:-48504920 None:intergenic
AGGAACTTGTTGAGAATAAA+GGG 0.371628 3.1:-48501524 None:intergenic
AGGAAAATTCTACCAAGATA+TGG 0.375212 3.1:+48503109 MS.gene05725:CDS
GTTTGGTTGGCATGAATCAT+TGG 0.378952 3.1:-48501637 None:intergenic
TTCCTGGTTTACCTGATACT+TGG 0.379922 3.1:+48505050 MS.gene05725:CDS
GACTGCAATCTGAGTAAATA+GGG 0.384362 3.1:-48505018 None:intergenic
CAAACAATGCACATGTTGTT+TGG 0.384544 3.1:-48501654 None:intergenic
ATATGGTATGTGCAGGACTA+TGG 0.396685 3.1:+48502651 MS.gene05725:intron
ATTCTTTGGGGAGGAATCAA+TGG 0.399441 3.1:+48501980 MS.gene05725:CDS
TTTACTCAGATTGCAGTCAT+TGG 0.410699 3.1:+48505023 MS.gene05725:CDS
TATCTAAGGTAATCAGAATC+AGG 0.416018 3.1:-48501772 None:intergenic
ATCTATCATGGGACAGTTTG+AGG 0.433290 3.1:-48501719 None:intergenic
GGGAAAAGTATAATGTTTGT+TGG 0.435721 3.1:+48501955 MS.gene05725:CDS
CTGCTAAACGATCCATATCT+TGG 0.436602 3.1:-48503121 None:intergenic
ACACAAACACAACTTGTTAG+AGG 0.438324 3.1:+48502042 MS.gene05725:CDS
GTTTCAAAACTCTCTTCCCT+TGG 0.440343 3.1:-48502725 None:intergenic
AAGAAGCTATGTAGCATTGA+AGG 0.446758 3.1:-48501561 None:intergenic
AGCCCAAATGAATGGACTTC+TGG 0.450002 3.1:+48504767 MS.gene05725:intron
CAGTTCAACTGTAAAATGTT+TGG 0.450871 3.1:+48501745 MS.gene05725:CDS
AGAACCAAGGTGTTCTTCCT+AGG 0.470720 3.1:+48503203 MS.gene05725:CDS
GTGGTCTCCATCTATATCTA+AGG 0.473099 3.1:-48501786 None:intergenic
GTTGAACTGTGGATCTATCA+TGG 0.481721 3.1:-48501731 None:intergenic
CAGTTTCTTATGGGAATGAA+AGG 0.490138 3.1:+48501934 MS.gene05725:CDS
TGATATGCAGCCCAAATGAA+TGG 0.490153 3.1:+48504759 MS.gene05725:intron
CACTTAACTGTGAGCTTCCA+AGG 0.492390 3.1:+48501806 MS.gene05725:CDS
ACACATGTAGGATCTCTTCA+AGG 0.496334 3.1:+48502822 MS.gene05725:CDS
CTTGGAAGCTCACAGTTAAG+TGG 0.496671 3.1:-48501805 None:intergenic
AAGGAGCTTTATAAAATGAA+AGG 0.497440 3.1:-48502680 None:intergenic
TGAGAATAAAGGGTTAACAT+TGG 0.497995 3.1:-48501514 None:intergenic
CATTTAATTCTGGACATTGG+TGG 0.500233 3.1:+48502799 MS.gene05725:CDS
CAATGCACATGTTGTTTGGT+TGG 0.504911 3.1:-48501650 None:intergenic
TCGTTTAGCAGCAATGGAAA+GGG 0.507114 3.1:+48503133 MS.gene05725:CDS
GCACACCCTTCAGTTTATAG+TGG 0.509577 3.1:+48504968 MS.gene05725:CDS
TTCTTTGGGGAGGAATCAAT+GGG 0.518021 3.1:+48501981 MS.gene05725:CDS
TGTCATTTAATTCTGGACAT+TGG 0.520728 3.1:+48502796 MS.gene05725:CDS
TCATGGGACAGTTTGAGGAT+TGG 0.521077 3.1:-48501714 None:intergenic
TATGGATCGTTTAGCAGCAA+TGG 0.521634 3.1:+48503127 MS.gene05725:CDS
GGCTAATTGGGTGGACAGCA+AGG 0.525841 3.1:+48503169 MS.gene05725:CDS
GCATCATCAAAGAATTGCTA+TGG 0.526714 3.1:+48504800 MS.gene05725:CDS
GATGGTGGAGATTGTTGTGG+TGG 0.527010 3.1:-48501609 None:intergenic
AAATCACCACTATAAACTGA+AGG 0.527895 3.1:-48504974 None:intergenic
AGGAGCTTTATAAAATGAAA+GGG 0.531001 3.1:-48502679 None:intergenic
ATTTGTTCTGGAAATACACC+AGG 0.540993 3.1:-48504860 None:intergenic
TATGTGATGGAGAAATTCCT+AGG 0.544650 3.1:-48503220 None:intergenic
AGTCCCTACTTGATATGTGA+TGG 0.545990 3.1:-48503233 None:intergenic
AGTTTCTTATGGGAATGAAA+GGG 0.547253 3.1:+48501935 MS.gene05725:CDS
ATCGTTTAGCAGCAATGGAA+AGG 0.548064 3.1:+48503132 MS.gene05725:CDS
AAAACTCACCAAGAATCTAA+AGG 0.549309 3.1:-48502079 None:intergenic
TGTATTTCCAGAACAAATGA+AGG 0.550178 3.1:+48504865 MS.gene05725:CDS
GCTGTGCCACTACTACTGAT+TGG 0.558530 3.1:-48504833 None:intergenic
ATAGGGTTAGCTCTTTGTTG+AGG 0.562146 3.1:-48505001 None:intergenic
TTTGATGGTGGAGATTGTTG+TGG 0.564274 3.1:-48501612 None:intergenic
TAGATGTAAATGGAGATGCT+TGG 0.565293 3.1:+48502757 MS.gene05725:CDS
GTGCATTGTTTGTAGGAACA+TGG 0.567892 3.1:+48501665 MS.gene05725:CDS
ACAAGTTGTGTTTGTGTCTG+AGG 0.573525 3.1:-48502036 None:intergenic
TGTTTGTTGGTGATTCTTTG+GGG 0.574043 3.1:+48501968 MS.gene05725:CDS
AAGAATCTAAAGGTTGAAAG+TGG 0.576820 3.1:-48502069 None:intergenic
TTGTTGGTGATTCTTTGGGG+AGG 0.582424 3.1:+48501971 MS.gene05725:CDS
TGCATTGTTTGTAGGAACAT+GGG 0.591865 3.1:+48501666 MS.gene05725:CDS
TGAAGGGTAAGTATGAAAAG+AGG 0.600167 3.1:-48501544 None:intergenic
AGAAGCTATGTAGCATTGAA+GGG 0.600751 3.1:-48501560 None:intergenic
TTGAACTGTGGATCTATCAT+GGG 0.601555 3.1:-48501730 None:intergenic
GGACATTGGTGGACACATGT+AGG 0.602746 3.1:+48502810 MS.gene05725:CDS
TTCCAAGTATCAGGTAAACC+AGG 0.603992 3.1:-48505052 None:intergenic
GTAGATGTTGATGTGGTCCA+AGG 0.604394 3.1:+48502708 MS.gene05725:CDS
GAATCATTGGTCTTTGATGG+TGG 0.607358 3.1:-48501624 None:intergenic
TGGGATTATATGGAATTAGG+AGG 0.611815 3.1:+48503089 MS.gene05725:CDS
ACTGGACCAATCAGTAGTAG+TGG 0.626043 3.1:+48504827 MS.gene05725:CDS
AATTCCTAGGAAGAACACCT+TGG 0.632807 3.1:-48503207 None:intergenic
CGTTTAGCAGCAATGGAAAG+GGG 0.639292 3.1:+48503134 MS.gene05725:CDS
AGTAGTGGCACAGCATACCC+TGG 0.640337 3.1:+48504842 MS.gene05725:CDS
CACATGTAGGATCTCTTCAA+GGG 0.641238 3.1:+48502823 MS.gene05725:CDS
AATCACCACTATAAACTGAA+GGG 0.643095 3.1:-48504973 None:intergenic
ACAAATGAAGGTCATAGACA+TGG 0.660441 3.1:+48504877 MS.gene05725:CDS
TAGATGTTGATGTGGTCCAA+GGG 0.683419 3.1:+48502709 MS.gene05725:CDS
GGTTGATAAAAGCAGAACCA+AGG 0.687362 3.1:+48503190 MS.gene05725:CDS
TTTGTTCTGGAAATACACCA+GGG 0.691599 3.1:-48504859 None:intergenic
TAATGTCTAGAAGATAAACA+GGG 0.755102 3.1:-48504919 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAATATTTTTAATTAAT+AGG + chr3.1:48502444-48502463 MS.gene05725:intron 0.0%
!!! AAATATATGTTTTATGAAAT+GGG - chr3.1:48504030-48504049 None:intergenic 10.0%
!!! ACATTATAATAAACATTTTT+TGG - chr3.1:48504073-48504092 None:intergenic 10.0%
!!! ATTTTTTTTTGTAATTTTGT+GGG + chr3.1:48504148-48504167 MS.gene05725:intron 10.0%
!!! TAAATATATGTTTTATGAAA+TGG - chr3.1:48504031-48504050 None:intergenic 10.0%
!! CCAAAAATAAATAAATATGT+CGG + chr3.1:48503907-48503926 MS.gene05725:intron 15.0%
!! TTAAATGAAATCAATATAGA+GGG - chr3.1:48502407-48502426 None:intergenic 15.0%
!! TTTAAATGAAATCAATATAG+AGG - chr3.1:48502408-48502427 None:intergenic 15.0%
!!! ACATCTATATTTAATTTCAA+TGG - chr3.1:48504216-48504235 None:intergenic 15.0%
!!! CATCTATATTTAATTTCAAT+GGG - chr3.1:48504215-48504234 None:intergenic 15.0%
!!! GATTTTTTTTTGTAATTTTG+TGG + chr3.1:48504147-48504166 MS.gene05725:intron 15.0%
!! AAAAAAAAATCAGCAGTATT+GGG - chr3.1:48504138-48504157 None:intergenic 20.0%
!! AAAATGGTCAAAAAATTACA+AGG - chr3.1:48502945-48502964 None:intergenic 20.0%
!! ACATATATTTAAATGTCACA+TGG + chr3.1:48504040-48504059 MS.gene05725:intron 20.0%
!! ATTAGAGTAATTTGATTGTT+GGG + chr3.1:48503047-48503066 MS.gene05725:intron 20.0%
!! GATAAATATAGACATATACT+AGG - chr3.1:48504711-48504730 None:intergenic 20.0%
!!! AAAAAATCCGTACTTTTATA+GGG - chr3.1:48502913-48502932 None:intergenic 20.0%
!!! ATTGATTTCATTTAAAACCA+GGG + chr3.1:48502412-48502431 MS.gene05725:intron 20.0%
!!! ATTTTATAAAGCTCCTTTTT+TGG + chr3.1:48502686-48502705 MS.gene05725:CDS 20.0%
!!! CAATGTTTTTGTTGAAATTA+TGG - chr3.1:48504342-48504361 None:intergenic 20.0%
!!! CCGACATATTTATTTATTTT+TGG - chr3.1:48503910-48503929 None:intergenic 20.0%
!!! GTGAACAAAAATTGATTTTT+TGG - chr3.1:48502362-48502381 None:intergenic 20.0%
!!! TAAAAAATCCGTACTTTTAT+AGG - chr3.1:48502914-48502933 None:intergenic 20.0%
!!! TAGTAAGCAAGAATATTTTT+CGG + chr3.1:48502975-48502994 MS.gene05725:intron 20.0%
!!! TATTGATTTCATTTAAAACC+AGG + chr3.1:48502411-48502430 MS.gene05725:intron 20.0%
!!! TGAAGTTTTTGTTGAAATTA+TGG - chr3.1:48504503-48504522 None:intergenic 20.0%
! AGACATATACTAGGTTAAAT+AGG - chr3.1:48504702-48504721 None:intergenic 25.0%
! ATTGCTATGAAAGATTTCTA+AGG + chr3.1:48503325-48503344 MS.gene05725:intron 25.0%
! CAAAAAAAAATCAGCAGTAT+TGG - chr3.1:48504139-48504158 None:intergenic 25.0%
! CATGAACAAATGTTTATAAG+TGG + chr3.1:48502155-48502174 MS.gene05725:intron 25.0%
! CTTATGAGAAAATATCAAGT+TGG - chr3.1:48502311-48502330 None:intergenic 25.0%
! GAAAAATATTCTTGCTTACT+AGG - chr3.1:48502976-48502995 None:intergenic 25.0%
! GACATATACTAGGTTAAATA+GGG - chr3.1:48504701-48504720 None:intergenic 25.0%
! GATTAGAGTAATTTGATTGT+TGG + chr3.1:48503046-48503065 MS.gene05725:intron 25.0%
! TAAGCCATTTATATATGTCT+TGG + chr3.1:48502524-48502543 MS.gene05725:intron 25.0%
! TAATGTATGAGAGAGAAATA+AGG - chr3.1:48503289-48503308 None:intergenic 25.0%
! TAATGTCTAGAAGATAAACA+GGG - chr3.1:48504922-48504941 None:intergenic 25.0%
! TAGTACATAACATTTGTAAG+TGG + chr3.1:48503991-48504010 MS.gene05725:intron 25.0%
! TAGTCCAAGACATATATAAA+TGG - chr3.1:48502531-48502550 None:intergenic 25.0%
! TATATTTAAATGTCACATGG+AGG + chr3.1:48504043-48504062 MS.gene05725:intron 25.0%
! TCTAGACATTACAATGTTAT+CGG + chr3.1:48504931-48504950 MS.gene05725:CDS 25.0%
! TCTGATATTCTGATTCAATT+TGG + chr3.1:48503845-48503864 MS.gene05725:intron 25.0%
! TTAGAGTAATTTGATTGTTG+GGG + chr3.1:48503048-48503067 MS.gene05725:intron 25.0%
!! AAGGAGCTTTATAAAATGAA+AGG - chr3.1:48502683-48502702 None:intergenic 25.0%
!! AGGAGCTTTATAAAATGAAA+GGG - chr3.1:48502682-48502701 None:intergenic 25.0%
!! AGTTTTTGTTGAAATTATGG+TGG - chr3.1:48504500-48504519 None:intergenic 25.0%
!! CAATTTTTGTTCACAAGAAA+AGG + chr3.1:48502368-48502387 MS.gene05725:intron 25.0%
!! GATATCTTGTCATTTAATTC+TGG + chr3.1:48502789-48502808 MS.gene05725:CDS 25.0%
!! TATATGTTTTATGAAATGGG+TGG - chr3.1:48504027-48504046 None:intergenic 25.0%
!! TTACTATGATTTGCATTCAA+GGG + chr3.1:48503453-48503472 MS.gene05725:intron 25.0%
!! TTGATTTCATTTAAAACCAG+GGG + chr3.1:48502413-48502432 MS.gene05725:intron 25.0%
!! TTTACTATGATTTGCATTCA+AGG + chr3.1:48503452-48503471 MS.gene05725:intron 25.0%
!!! TAGTTTTTTCAAAGATTCGT+TGG + chr3.1:48502259-48502278 MS.gene05725:intron 25.0%
!!! TATCAACACGTTTTAAACTT+TGG - chr3.1:48504429-48504448 None:intergenic 25.0%
!!! TCAACACATGTTTTAAACTT+TGG - chr3.1:48504266-48504285 None:intergenic 25.0%
!!! TGTTTTTGTTGAAATTATGG+TGG - chr3.1:48504339-48504358 None:intergenic 25.0%
!!! TTTTTTAGCTTGTTGTTGTT+AGG + chr3.1:48501899-48501918 MS.gene05725:intron 25.0%
AAAACTCACCAAGAATCTAA+AGG - chr3.1:48502082-48502101 None:intergenic 30.0%
AAATCACCACTATAAACTGA+AGG - chr3.1:48504977-48504996 None:intergenic 30.0%
AAATGTGAGTGATAGAGATA+GGG - chr3.1:48503752-48503771 None:intergenic 30.0%
AACAATTCATTCCAAGTATC+AGG - chr3.1:48505064-48505083 None:intergenic 30.0%
AAGAATCTAAAGGTTGAAAG+TGG - chr3.1:48502072-48502091 None:intergenic 30.0%
AATATTCTTGCTTACTAGGT+AGG - chr3.1:48502972-48502991 None:intergenic 30.0%
AATCACCACTATAAACTGAA+GGG - chr3.1:48504976-48504995 None:intergenic 30.0%
AATGGAGTTCAGTTTCTTAT+GGG + chr3.1:48501925-48501944 MS.gene05725:CDS 30.0%
ACAAGTATCTAGTGGAAAAA+AGG + chr3.1:48502467-48502486 MS.gene05725:intron 30.0%
ACATCAACATCTACCAAAAA+AGG - chr3.1:48502702-48502721 None:intergenic 30.0%
ACTTTCAACCTTTAGATTCT+TGG + chr3.1:48502071-48502090 MS.gene05725:CDS 30.0%
AGGAAAATTCTACCAAGATA+TGG + chr3.1:48503109-48503128 MS.gene05725:CDS 30.0%
AGTTTCTTATGGGAATGAAA+GGG + chr3.1:48501935-48501954 MS.gene05725:CDS 30.0%
ATATTCTTGCTTACTAGGTA+GGG - chr3.1:48502971-48502990 None:intergenic 30.0%
ATCATCTTAAAATGCGTAGA+TGG - chr3.1:48502227-48502246 None:intergenic 30.0%
ATGTACTAGTAAGTAGTTCA+TGG - chr3.1:48503979-48503998 None:intergenic 30.0%
CAGTTCAACTGTAAAATGTT+TGG + chr3.1:48501745-48501764 MS.gene05725:CDS 30.0%
CATATCTGGTAGTTATCTAT+CGG + chr3.1:48503570-48503589 MS.gene05725:intron 30.0%
CCTAAGCTTAGAAATATCTT+TGG + chr3.1:48503413-48503432 MS.gene05725:intron 30.0%
CCTTAAATAGATCTCAAACT+CGG + chr3.1:48504637-48504656 MS.gene05725:intron 30.0%
CTGATTACCTTAGATATAGA+TGG + chr3.1:48501779-48501798 MS.gene05725:CDS 30.0%
CTTGAAACTCAAATTGCAAT+GGG - chr3.1:48503499-48503518 None:intergenic 30.0%
GAACAAATGTTTATAAGTGG+TGG + chr3.1:48502158-48502177 MS.gene05725:intron 30.0%
GTAATGTCTAGAAGATAAAC+AGG - chr3.1:48504923-48504942 None:intergenic 30.0%
TACATAACATTTGTAAGTGG+AGG + chr3.1:48503994-48504013 MS.gene05725:intron 30.0%
TAGAGTAATTTGATTGTTGG+GGG + chr3.1:48503049-48503068 MS.gene05725:intron 30.0%
TCTATCACTCACATTTGTAT+GGG + chr3.1:48503756-48503775 MS.gene05725:intron 30.0%
TGAATCAGAATATCAGATTG+TGG - chr3.1:48503842-48503861 None:intergenic 30.0%
TGTATTTCCAGAACAAATGA+AGG + chr3.1:48504865-48504884 MS.gene05725:CDS 30.0%
TTAATAGGACAAGTATCTAG+TGG + chr3.1:48502459-48502478 MS.gene05725:intron 30.0%
TTACTAGGTAGGGATTAAAA+TGG - chr3.1:48502961-48502980 None:intergenic 30.0%
TTTCAGTCCCTATAAAAGTA+CGG + chr3.1:48502903-48502922 MS.gene05725:intron 30.0%
TTTGATTGTGTTTGTTCAGT+TGG + chr3.1:48503380-48503399 MS.gene05725:intron 30.0%
! AACATTTTACAGTTGAACTG+TGG - chr3.1:48501745-48501764 None:intergenic 30.0%
! AGTTTGAGGATTGGTAAAAA+GGG - chr3.1:48501708-48501727 None:intergenic 30.0%
! CCAAAGATATTTCTAAGCTT+AGG - chr3.1:48503416-48503435 None:intergenic 30.0%
! CGAGTTTGAGATCTATTTAA+GGG - chr3.1:48504639-48504658 None:intergenic 30.0%
! GGGAAAAGTATAATGTTTGT+TGG + chr3.1:48501955-48501974 MS.gene05725:CDS 30.0%
! TATCTAAGGTAATCAGAATC+AGG - chr3.1:48501775-48501794 None:intergenic 30.0%
! TCTAAAAACATGGGCTAATT+GGG + chr3.1:48503157-48503176 MS.gene05725:CDS 30.0%
! TGTCAAGAAACACTTTTGTT+TGG - chr3.1:48502623-48502642 None:intergenic 30.0%
! TGTCATTTAATTCTGGACAT+TGG + chr3.1:48502796-48502815 MS.gene05725:CDS 30.0%
! TTTAAGACTGTTTGTTACCT+TGG - chr3.1:48501826-48501845 None:intergenic 30.0%
!! AATGTTTGTTGGTGATTCTT+TGG + chr3.1:48501966-48501985 MS.gene05725:CDS 30.0%
!! AGGAACTTGTTGAGAATAAA+GGG - chr3.1:48501527-48501546 None:intergenic 30.0%
!! ATCTTATAAGTCGGTTTTGT+AGG + chr3.1:48502189-48502208 MS.gene05725:intron 30.0%
!! ATGTTTGTTGGTGATTCTTT+GGG + chr3.1:48501967-48501986 MS.gene05725:CDS 30.0%
!! TCTTATAAGTCGGTTTTGTA+GGG + chr3.1:48502190-48502209 MS.gene05725:intron 30.0%
!! TGTTATGTGTTTTTGTGTTG+TGG + chr3.1:48501858-48501877 MS.gene05725:intron 30.0%
!! TTCTTGACAGAGTTTTGATA+TGG + chr3.1:48502634-48502653 MS.gene05725:intron 30.0%
!!! TGTGGTGTGTTTTTTTTGAT+TGG + chr3.1:48501876-48501895 MS.gene05725:intron 30.0%
!!! TTTTTTGGTAGATGTTGATG+TGG + chr3.1:48502701-48502720 MS.gene05725:CDS 30.0%
AAGAATTGCTATGGAGAAAC+TGG + chr3.1:48504809-48504828 MS.gene05725:CDS 35.0%
ACAAATGAAGGTCATAGACA+TGG + chr3.1:48504877-48504896 MS.gene05725:CDS 35.0%
ACAACATGTGCATTGTTTGT+AGG + chr3.1:48501658-48501677 MS.gene05725:CDS 35.0%
ACGTCAGTGTTCTATCATAT+CGG + chr3.1:48503024-48503043 MS.gene05725:intron 35.0%
AGGTGGGATTATATGGAATT+AGG + chr3.1:48503086-48503105 MS.gene05725:intron 35.0%
CAAACAATGCACATGTTGTT+TGG - chr3.1:48501657-48501676 None:intergenic 35.0%
CAAATGTGAGTGATAGAGAT+AGG - chr3.1:48503753-48503772 None:intergenic 35.0%
CAATGGAGTTCAGTTTCTTA+TGG + chr3.1:48501924-48501943 MS.gene05725:CDS 35.0%
CAGTTTCTTATGGGAATGAA+AGG + chr3.1:48501934-48501953 MS.gene05725:CDS 35.0%
CATTTAATTCTGGACATTGG+TGG + chr3.1:48502799-48502818 MS.gene05725:CDS 35.0%
CCTTGAAACTCAAATTGCAA+TGG - chr3.1:48503500-48503519 None:intergenic 35.0%
CTCTATCACTCACATTTGTA+TGG + chr3.1:48503755-48503774 MS.gene05725:intron 35.0%
GACTGCAATCTGAGTAAATA+GGG - chr3.1:48505021-48505040 None:intergenic 35.0%
GCATCATCAAAGAATTGCTA+TGG + chr3.1:48504800-48504819 MS.gene05725:CDS 35.0%
GGAAGTTGTAGAGATATTGA+GGG + chr3.1:48503650-48503669 MS.gene05725:intron 35.0%
GGTTTACAACGTTTCTCTTT+AGG + chr3.1:48503518-48503537 MS.gene05725:intron 35.0%
TAAAAAGGGTAAGTGTCATC+AGG - chr3.1:48501694-48501713 None:intergenic 35.0%
TAACTACCAGATATGTATCC+AGG - chr3.1:48503565-48503584 None:intergenic 35.0%
TACATCCGATAGATAACCAA+CGG + chr3.1:48503616-48503635 MS.gene05725:intron 35.0%
TAGATGTAAATGGAGATGCT+TGG + chr3.1:48502757-48502776 MS.gene05725:CDS 35.0%
TAGCTGTATGTTTGGATTGA+TGG + chr3.1:48504291-48504310 MS.gene05725:intron 35.0%
TATTAATAACCTAACTCCCC+TGG - chr3.1:48502432-48502451 None:intergenic 35.0%
TCTATGACCTTCATTTGTTC+TGG - chr3.1:48504875-48504894 None:intergenic 35.0%
TCTCCATCACATATCAAGTA+GGG + chr3.1:48503230-48503249 MS.gene05725:intron 35.0%
TGAAGGGTAAGTATGAAAAG+AGG - chr3.1:48501547-48501566 None:intergenic 35.0%
TGACGAGTTTGAAGAAATCA+CGG - chr3.1:48504556-48504575 None:intergenic 35.0%
TGACTGCAATCTGAGTAAAT+AGG - chr3.1:48505022-48505041 None:intergenic 35.0%
TGATACTCTAGCTGTATGTT+TGG + chr3.1:48504283-48504302 MS.gene05725:intron 35.0%
TGCATTGTTTGTAGGAACAT+GGG + chr3.1:48501666-48501685 MS.gene05725:CDS 35.0%
TGGAAGTTGTAGAGATATTG+AGG + chr3.1:48503649-48503668 MS.gene05725:intron 35.0%
TGGGATTATATGGAATTAGG+AGG + chr3.1:48503089-48503108 MS.gene05725:CDS 35.0%
TGTGGACAAGCAATAGAAAT+TGG - chr3.1:48503824-48503843 None:intergenic 35.0%
TTCTCCATCACATATCAAGT+AGG + chr3.1:48503229-48503248 MS.gene05725:intron 35.0%
TTGAACTGTGGATCTATCAT+GGG - chr3.1:48501733-48501752 None:intergenic 35.0%
TTTACTCAGATTGCAGTCAT+TGG + chr3.1:48505023-48505042 MS.gene05725:CDS 35.0%
! AAGAAGCTATGTAGCATTGA+AGG - chr3.1:48501564-48501583 None:intergenic 35.0%
! ACCGACTTATAAGATAAGCA+CGG - chr3.1:48502184-48502203 None:intergenic 35.0%
! AGAAGCTATGTAGCATTGAA+GGG - chr3.1:48501563-48501582 None:intergenic 35.0%
! CAGTTTGAGGATTGGTAAAA+AGG - chr3.1:48501709-48501728 None:intergenic 35.0%
! CCATTGCAATTTGAGTTTCA+AGG + chr3.1:48503497-48503516 MS.gene05725:intron 35.0%
! CCGAGTTTGAGATCTATTTA+AGG - chr3.1:48504640-48504659 None:intergenic 35.0%
! GTCTAAAAACATGGGCTAAT+TGG + chr3.1:48503156-48503175 MS.gene05725:CDS 35.0%
! GTGATTTGTGTGATAGAGAT+AGG - chr3.1:48503726-48503745 None:intergenic 35.0%
! TATGTGATGGAGAAATTCCT+AGG - chr3.1:48503223-48503242 None:intergenic 35.0%
!! ACACAAACACAACTTGTTAG+AGG + chr3.1:48502042-48502061 MS.gene05725:CDS 35.0%
!! AGTTTTGATATGGTATGTGC+AGG + chr3.1:48502644-48502663 MS.gene05725:intron 35.0%
!! ATTTGTTCTGGAAATACACC+AGG - chr3.1:48504863-48504882 None:intergenic 35.0%
!! CATGAATCATTGGTCTTTGA+TGG - chr3.1:48501630-48501649 None:intergenic 35.0%
!! CTTGTTGTTGTTAGGTTCAA+TGG + chr3.1:48501907-48501926 MS.gene05725:intron 35.0%
!! GAAAAAAGGTGTCATTGATG+GGG + chr3.1:48502481-48502500 MS.gene05725:intron 35.0%
!! GAGGAACTTGTTGAGAATAA+AGG - chr3.1:48501528-48501547 None:intergenic 35.0%
!! GGAAAAAAGGTGTCATTGAT+GGG + chr3.1:48502480-48502499 MS.gene05725:intron 35.0%
!! TGGAAAAAAGGTGTCATTGA+TGG + chr3.1:48502479-48502498 MS.gene05725:intron 35.0%
!! TGTTTGTTGGTGATTCTTTG+GGG + chr3.1:48501968-48501987 MS.gene05725:CDS 35.0%
!! TTTGTTCTGGAAATACACCA+GGG - chr3.1:48504862-48504881 None:intergenic 35.0%
!!! GGTTATTTTTAAGTGCAGGT+GGG + chr3.1:48503070-48503089 MS.gene05725:intron 35.0%
AATTCCTAGGAAGAACACCT+TGG - chr3.1:48503210-48503229 None:intergenic 40.0%
ACAAGTTGTGTTTGTGTCTG+AGG - chr3.1:48502039-48502058 None:intergenic 40.0%
ACACATGTAGGATCTCTTCA+AGG + chr3.1:48502822-48502841 MS.gene05725:CDS 40.0%
ACTCCAGAAGTCCATTCATT+TGG - chr3.1:48504773-48504792 None:intergenic 40.0%
AGGAAGCCTTAGAGATATTG+GGG - chr3.1:48503545-48503564 None:intergenic 40.0%
AGTCCCTACTTGATATGTGA+TGG - chr3.1:48503236-48503255 None:intergenic 40.0%
ATATGGTATGTGCAGGACTA+TGG + chr3.1:48502651-48502670 MS.gene05725:intron 40.0%
ATCACTCACATTTGTATGGG+CGG + chr3.1:48503759-48503778 MS.gene05725:intron 40.0%
ATCGTTTAGCAGCAATGGAA+AGG + chr3.1:48503132-48503151 MS.gene05725:CDS 40.0%
ATCTATCATGGGACAGTTTG+AGG - chr3.1:48501722-48501741 None:intergenic 40.0%
ATTGCAATGGGTCAAGAGAA+TGG - chr3.1:48503487-48503506 None:intergenic 40.0%
ATTGTTATCGTGATAGCAGG+TGG - chr3.1:48502286-48502305 None:intergenic 40.0%
CACATGTAGGATCTCTTCAA+GGG + chr3.1:48502823-48502842 MS.gene05725:CDS 40.0%
CAGGAAGCCTTAGAGATATT+GGG - chr3.1:48503546-48503565 None:intergenic 40.0%
CCAAGCAGTATTAGCACATT+CGG + chr3.1:48504095-48504114 MS.gene05725:intron 40.0%
CTCCAGAAGTCCATTCATTT+GGG - chr3.1:48504772-48504791 None:intergenic 40.0%
CTGCTAAACGATCCATATCT+TGG - chr3.1:48503124-48503143 None:intergenic 40.0%
CTGGAGGAGATAGATGTAAA+TGG + chr3.1:48502747-48502766 MS.gene05725:CDS 40.0%
GCCGTGCTTATCTTATAAGT+CGG + chr3.1:48502180-48502199 MS.gene05725:intron 40.0%
GGAAAGGGGTCTAAAAACAT+GGG + chr3.1:48503148-48503167 MS.gene05725:CDS 40.0%
GGTTAAATAGGGAATCTCTG+TGG - chr3.1:48504690-48504709 None:intergenic 40.0%
GTCATTGTTATCGTGATAGC+AGG - chr3.1:48502289-48502308 None:intergenic 40.0%
GTCGCAAGGCTTAAATTCAT+GGG + chr3.1:48503686-48503705 MS.gene05725:intron 40.0%
GTGCATTGTTTGTAGGAACA+TGG + chr3.1:48501665-48501684 MS.gene05725:CDS 40.0%
GTGGTCTCCATCTATATCTA+AGG - chr3.1:48501789-48501808 None:intergenic 40.0%
GTTGAACTGTGGATCTATCA+TGG - chr3.1:48501734-48501753 None:intergenic 40.0%
GTTTCAAAACTCTCTTCCCT+TGG - chr3.1:48502728-48502747 None:intergenic 40.0%
GTTTGGTTGGCATGAATCAT+TGG - chr3.1:48501640-48501659 None:intergenic 40.0%
TAAGTGCAGGTGGGATTATA+TGG + chr3.1:48503079-48503098 MS.gene05725:intron 40.0%
TAGATGTTGATGTGGTCCAA+GGG + chr3.1:48502709-48502728 MS.gene05725:CDS 40.0%
TATGGATCGTTTAGCAGCAA+TGG + chr3.1:48503127-48503146 MS.gene05725:CDS 40.0%
TCATTTGGGCTGCATATCAA+TGG - chr3.1:48504758-48504777 None:intergenic 40.0%
TCGTTTAGCAGCAATGGAAA+GGG + chr3.1:48503133-48503152 MS.gene05725:CDS 40.0%
TCTGGTAGTTATCTATCGGA+TGG + chr3.1:48503574-48503593 MS.gene05725:intron 40.0%
TGATATGCAGCCCAAATGAA+TGG + chr3.1:48504759-48504778 MS.gene05725:intron 40.0%
TGGAAAGGGGTCTAAAAACA+TGG + chr3.1:48503147-48503166 MS.gene05725:CDS 40.0%
TTCCAAGTATCAGGTAAACC+AGG - chr3.1:48505055-48505074 None:intergenic 40.0%
TTCCTGGTTTACCTGATACT+TGG + chr3.1:48505050-48505069 MS.gene05725:CDS 40.0%
TTGCAATGGGTCAAGAGAAT+GGG - chr3.1:48503486-48503505 None:intergenic 40.0%
! AAAAACATGGGCTAATTGGG+TGG + chr3.1:48503160-48503179 MS.gene05725:CDS 40.0%
! ATTCTTTGGGGAGGAATCAA+TGG + chr3.1:48501980-48501999 MS.gene05725:CDS 40.0%
! CAATGCACATGTTGTTTGGT+TGG - chr3.1:48501653-48501672 None:intergenic 40.0%
! CACATCCGTTGGTTATCTAT+CGG - chr3.1:48503624-48503643 None:intergenic 40.0%
! CATTTAAAACCAGGGGAGTT+AGG + chr3.1:48502420-48502439 MS.gene05725:intron 40.0%
! GAACTTTTCTCTACATGTGC+AGG + chr3.1:48503869-48503888 MS.gene05725:intron 40.0%
! TTCTTTGGGGAGGAATCAAT+GGG + chr3.1:48501981-48502000 MS.gene05725:CDS 40.0%
!! ATAGGGTTAGCTCTTTGTTG+AGG - chr3.1:48505004-48505023 None:intergenic 40.0%
!! GAATCATTGGTCTTTGATGG+TGG - chr3.1:48501627-48501646 None:intergenic 40.0%
!! GGTTGATAAAAGCAGAACCA+AGG + chr3.1:48503190-48503209 MS.gene05725:CDS 40.0%
!! TTGGACTAGTTGATGCAAAC+TGG + chr3.1:48502543-48502562 MS.gene05725:intron 40.0%
!! TTTGATGGTGGAGATTGTTG+TGG - chr3.1:48501615-48501634 None:intergenic 40.0%
!!! AGGGAAGAGAGTTTTGAAAC+TGG + chr3.1:48502728-48502747 MS.gene05725:CDS 40.0%
!!! GAAGAGAGTTTTGAAACTGG+AGG + chr3.1:48502731-48502750 MS.gene05725:CDS 40.0%
!!! GGGTTATTTTTAAGTGCAGG+TGG + chr3.1:48503069-48503088 MS.gene05725:intron 40.0%
!!! TGGGGGTTATTTTTAAGTGC+AGG + chr3.1:48503066-48503085 MS.gene05725:intron 40.0%
ACTGGACCAATCAGTAGTAG+TGG + chr3.1:48504827-48504846 MS.gene05725:CDS 45.0%
AGGCTTCCTGGATACATATC+TGG + chr3.1:48503556-48503575 MS.gene05725:intron 45.0%
CACTTAACTGTGAGCTTCCA+AGG + chr3.1:48501806-48501825 MS.gene05725:CDS 45.0%
CCAGGAAGCCTTAGAGATAT+TGG - chr3.1:48503547-48503566 None:intergenic 45.0%
CCGAATGTGCTAATACTGCT+TGG - chr3.1:48504098-48504117 None:intergenic 45.0%
CGAGTTTGAAGAAATCACGG+TGG - chr3.1:48504553-48504572 None:intergenic 45.0%
CGATAGATAACCAACGGATG+TGG + chr3.1:48503622-48503641 MS.gene05725:intron 45.0%
CGTCGCAAGGCTTAAATTCA+TGG + chr3.1:48503685-48503704 MS.gene05725:intron 45.0%
CGTTTAGCAGCAATGGAAAG+GGG + chr3.1:48503134-48503153 MS.gene05725:CDS 45.0%
CTCTACATGTGCAGGATCAT+GGG + chr3.1:48503877-48503896 MS.gene05725:intron 45.0%
CTTGGAAGCTCACAGTTAAG+TGG - chr3.1:48501808-48501827 None:intergenic 45.0%
GATTTGCATTCAAGGGCTTG+AGG + chr3.1:48503460-48503479 MS.gene05725:intron 45.0%
GCACACCCTTCAGTTTATAG+TGG + chr3.1:48504968-48504987 MS.gene05725:CDS 45.0%
GGAAGCCTTAGAGATATTGG+GGG - chr3.1:48503544-48503563 None:intergenic 45.0%
GTAGATGTTGATGTGGTCCA+AGG + chr3.1:48502708-48502727 MS.gene05725:CDS 45.0%
TAACCAACGGATGTGGATCT+TGG + chr3.1:48503629-48503648 MS.gene05725:intron 45.0%
TCACTCACATTTGTATGGGC+GGG + chr3.1:48503760-48503779 MS.gene05725:intron 45.0%
TCATGGGACAGTTTGAGGAT+TGG - chr3.1:48501717-48501736 None:intergenic 45.0%
TCTCTACATGTGCAGGATCA+TGG + chr3.1:48503876-48503895 MS.gene05725:intron 45.0%
TGTAGGATCTCTTCAAGGGT+AGG + chr3.1:48502827-48502846 MS.gene05725:intron 45.0%
TTAGGCCCCCAATATCTCTA+AGG + chr3.1:48503536-48503555 MS.gene05725:intron 45.0%
! AGAACCAAGGTGTTCTTCCT+AGG + chr3.1:48503203-48503222 MS.gene05725:CDS 45.0%
! AGCCCAAATGAATGGACTTC+TGG + chr3.1:48504767-48504786 MS.gene05725:intron 45.0%
!! CCAATATCTCTAAGGCTTCC+TGG + chr3.1:48503544-48503563 MS.gene05725:intron 45.0%
!! TTGTTGGTGATTCTTTGGGG+AGG + chr3.1:48501971-48501990 MS.gene05725:CDS 45.0%
!! ATCTTATATTAAAAAATTTA+GGG + chr3.1:48502870-48502889 MS.gene05725:intron 5.0%
!! TATCTTATATTAAAAAATTT+AGG + chr3.1:48502869-48502888 MS.gene05725:intron 5.0%
!!! TTTTTAATATAAGATAATTA+AGG - chr3.1:48502865-48502884 None:intergenic 5.0%
CTTCCAAGATCCACATCCGT+TGG - chr3.1:48503635-48503654 None:intergenic 50.0%
GATCAGATGTGTCCCGATGT+CGG + chr3.1:48502997-48503016 MS.gene05725:intron 50.0%
GCCTAAAGAGAAACGTCGCA+AGG + chr3.1:48503672-48503691 MS.gene05725:intron 50.0%
GCCTTGCGACGTTTCTCTTT+AGG - chr3.1:48503676-48503695 None:intergenic 50.0%
! GCTGTGCCACTACTACTGAT+TGG - chr3.1:48504836-48504855 None:intergenic 50.0%
! GGACATTGGTGGACACATGT+AGG + chr3.1:48502810-48502829 MS.gene05725:CDS 50.0%
! TGCAGTCATTGGTGTCTTCC+TGG + chr3.1:48505034-48505053 MS.gene05725:CDS 50.0%
!! GATGGTGGAGATTGTTGTGG+TGG - chr3.1:48501612-48501631 None:intergenic 50.0%
ACTGACGTGTCTCCGACATC+GGG - chr3.1:48503012-48503031 None:intergenic 55.0%
AGTAGTGGCACAGCATACCC+TGG + chr3.1:48504842-48504861 MS.gene05725:CDS 55.0%
CACTGACGTGTCTCCGACAT+CGG - chr3.1:48503013-48503032 None:intergenic 55.0%
GGCTAATTGGGTGGACAGCA+AGG + chr3.1:48503169-48503188 MS.gene05725:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 48501517 48505105 48501517 ID=MS.gene05725
chr3.1 mRNA 48501517 48505105 48501517 ID=MS.gene05725.t1;Parent=MS.gene05725
chr3.1 exon 48501517 48501827 48501517 ID=MS.gene05725.t1.exon1;Parent=MS.gene05725.t1
chr3.1 CDS 48501517 48501827 48501517 ID=cds.MS.gene05725.t1;Parent=MS.gene05725.t1
chr3.1 exon 48501921 48502092 48501921 ID=MS.gene05725.t1.exon2;Parent=MS.gene05725.t1
chr3.1 CDS 48501921 48502092 48501921 ID=cds.MS.gene05725.t1;Parent=MS.gene05725.t1
chr3.1 exon 48502666 48502844 48502666 ID=MS.gene05725.t1.exon3;Parent=MS.gene05725.t1
chr3.1 CDS 48502666 48502844 48502666 ID=cds.MS.gene05725.t1;Parent=MS.gene05725.t1
chr3.1 exon 48503088 48503246 48503088 ID=MS.gene05725.t1.exon4;Parent=MS.gene05725.t1
chr3.1 CDS 48503088 48503246 48503088 ID=cds.MS.gene05725.t1;Parent=MS.gene05725.t1
chr3.1 exon 48504769 48505105 48504769 ID=MS.gene05725.t1.exon5;Parent=MS.gene05725.t1
chr3.1 CDS 48504769 48505105 48504769 ID=cds.MS.gene05725.t1;Parent=MS.gene05725.t1
Gene Sequence

>MS.gene05725

ATGTTAACCCTTTATTCTCAACAAGTTCCTCTTTTCATACTTACCCTTCAATGCTACATAGCTTCTTCAGCTATACTATACAGTTTGAAACACCACCACAACAATCTCCACCATCAAAGACCAATGATTCATGCCAACCAAACAACATGTGCATTGTTTGTAGGAACATGGGTTCCTGATGACACTTACCCTTTTTACCAATCCTCAAACTGTCCCATGATAGATCCACAGTTCAACTGTAAAATGTTTGGTCGTCCTGATTCTGATTACCTTAGATATAGATGGAGACCACTTAACTGTGAGCTTCCAAGGTAACAAACAGTCTTAAAGATTGTATTTTTTGTTATGTGTTTTTGTGTTGTGGTGTGTTTTTTTTGATTGGTTTTTTAGCTTGTTGTTGTTAGGTTCAATGGAGTTCAGTTTCTTATGGGAATGAAAGGGAAAAGTATAATGTTTGTTGGTGATTCTTTGGGGAGGAATCAATGGGAATCATTGATTTGTATGATATATGCTGAAGTTCCTCAGACACAAACACAACTTGTTAGAGGAGTGCCACTTTCAACCTTTAGATTCTTGGTGAGTTTTTTTTGACACTTTCTAGATGCATCTCAAAATTAGATTTAATTCATGTGATATGACATGAACAAATGTTTATAAGTGGTGGCCGTGCTTATCTTATAAGTCGGTTTTGTAGGGTTGTGTTAAGACCATCTACGCATTTTAAGATGATATGATGATATCATAGTTTTTTCAAAGATTCGTTGGACCACCTGCTATCACGATAACAATGACCAACTTGATATTTTCTCATAAGTTTCAATTTTTATAATTGAGATTTTATGCCAAAAAATCAATTTTTGTTCACAAGAAAAGGTAAAGTTGTTTAACCCTCTATATTGATTTCATTTAAAACCAGGGGAGTTAGGTTATTAATATTTTTAATTAATAGGACAAGTATCTAGTGGAAAAAAGGTGTCATTGATGGGGACACAAAAGTATAGTACTATTAAGCCATTTATATATGTCTTGGACTAGTTGATGCAAACTGGAAGCAAATCAGTGTTCATGTTGATGTATGCATAAAAAGTCACATTTTACAGCTGCCAAACAAAAGTGTTTCTTGACAGAGTTTTGATATGGTATGTGCAGGACTATGGTGTGACCCTTTCATTTTATAAAGCTCCTTTTTTGGTAGATGTTGATGTGGTCCAAGGGAAGAGAGTTTTGAAACTGGAGGAGATAGATGTAAATGGAGATGCTTGGAAGAATGTTGATATCTTGTCATTTAATTCTGGACATTGGTGGACACATGTAGGATCTCTTCAAGGGTAGGCAAATATCTTTTCCTTAATTATCTTATATTAAAAAATTTAGGGTTAAATAGATTTTCAGTCCCTATAAAAGTACGGATTTTTTAAGTTAAGTCCTTGTAATTTTTTGACCATTTTAATCCCTACCTAGTAAGCAAGAATATTTTTCGGATCAGATGTGTCCCGATGTCGGAGACACGTCAGTGTTCTATCATATCGGATTAGAGTAATTTGATTGTTGGGGGTTATTTTTAAGTGCAGGTGGGATTATATGGAATTAGGAGGAAAATTCTACCAAGATATGGATCGTTTAGCAGCAATGGAAAGGGGTCTAAAAACATGGGCTAATTGGGTGGACAGCAAGGTTGATAAAAGCAGAACCAAGGTGTTCTTCCTAGGAATTTCTCCATCACATATCAAGTAGGGACTCTGACTCTCTCTCTCTCACTACTGTCTTGACCTTATTTCTCTCTCATACATTATTGTACTATAAACATTATTGCTATGAAAGATTTCTAAGGCTTATTATTGCTATGAAAGATTTCTAAGGCTTTTTGATTGTGTTTGTTCAGTTGGAAAAGCGTATCCTAAGCTTAGAAATATCTTTGGTTTATAAATTATGTAATTTACTATGATTTGCATTCAAGGGCTTGAGGCCCATTCTCTTGACCCATTGCAATTTGAGTTTCAAGGTTTACAACGTTTCTCTTTAGGCCCCCAATATCTCTAAGGCTTCCTGGATACATATCTGGTAGTTATCTATCGGATGGACGTTTTAAAAAAAAAATGTACATCCGATAGATAACCAACGGATGTGGATCTTGGAAGTTGTAGAGATATTGAGGGCCTAAAGAGAAACGTCGCAAGGCTTAAATTCATGGGCTTGTTGTGTCAGTCCTATCTCTATCACACAAATCACAAGCCCTATCTCTATCACTCACATTTGTATGGGCGGGTAATAATGTATTATATGAGTAGCATATCATAATGCTCACCAATTTCTATTGCTTGTCCACAATCTGATATTCTGATTCAATTTGGAGAACTTTTCTCTACATGTGCAGGATCATGGGAATAATACCAAAAATAAATAAATATGTCGGTGAAATAAAATGCATGCATCATAGATAGATATATACTATATTTTTACCATGAACTACTTACTAGTACATAACATTTGTAAGTGGAGGTCTTGAGCCACCCATTTCATAAAACATATATTTAAATGTCACATGGAGGAAGACCAAAAAATGTTTATTATAATGTGACCAAGCAGTATTAGCACATTCGGAGTGTCTTAGTCTGCATCCCAATACTGCTGATTTTTTTTTGTAATTTTGTGGGTTCATTCATCACAATTTTTCTATCACTGTGACTAGCATTGTCCCATTGAAATTAAATATAGATGTGAAACGACATAAGAAAGTTGTTGTGTTCCAAAGTTTAAAACATGTGTTGATACTCTAGCTGTATGTTTGGATTGATGGTGAGTTTGACGTTGTACTCGTGCCACCATAATTTCAACAAAAACATTGTGAGTAGCATTGTCCCATTGAAATTAAATATAGATGTGAAACGACATAAAAAAGTTGTTGTGTTCCAAAGTTTAAAACGTGTTGATACTCTAGCTGTATGTTTGGATTGATGGTGAGTTTGACGTTGTACTCGTGCCACCATAATTTCAACAAAAACTTCATTTTACAGCTTCAACAAAATCACAGCACCACCGTGATTTCTTCAAACTCGTCACGAGTCTGAACATACACTAAATATATTCGTACAGAAAAACATCTGTGAAGAGAGTTCAAACCCTTAAATAGATCTCAAACTCGGTTACTTTTTGAGATTAATCTCTAGTTTCCACAGAGATTCCCTATTTAACCTAGTATATGTCTATATTTATCTATAAGCTATGCTAAAACTCAATGCCATTGATATGCAGCCCAAATGAATGGACTTCTGGAGTAACAACAGCATCATCAAAGAATTGCTATGGAGAAACTGGACCAATCAGTAGTAGTGGCACAGCATACCCTGGTGTATTTCCAGAACAAATGAAGGTCATAGACATGGTGATTAGAGAAATGAACTACCCTGTTTATCTTCTAGACATTACAATGTTATCGGCATTTAGAAAAGACGCACACCCTTCAGTTTATAGTGGTGATTTGAGTCCTCAACAAAGAGCTAACCCTATTTACTCAGATTGCAGTCATTGGTGTCTTCCTGGTTTACCTGATACTTGGAATGAATTGTTCTATACTACATTGTTCTACTAA
Protein sequence

>MS.gene05725.t1

MLTLYSQQVPLFILTLQCYIASSAILYSLKHHHNNLHHQRPMIHANQTTCALFVGTWVPDDTYPFYQSSNCPMIDPQFNCKMFGRPDSDYLRYRWRPLNCELPRFNGVQFLMGMKGKSIMFVGDSLGRNQWESLICMIYAEVPQTQTQLVRGVPLSTFRFLDYGVTLSFYKAPFLVDVDVVQGKRVLKLEEIDVNGDAWKNVDILSFNSGHWWTHVGSLQGWDYMELGGKFYQDMDRLAAMERGLKTWANWVDSKVDKSRTKVFFLGISPSHINPNEWTSGVTTASSKNCYGETGPISSSGTAYPGVFPEQMKVIDMVIREMNYPVYLLDITMLSAFRKDAHPSVYSGDLSPQQRANPIYSDCSHWCLPGLPDTWNELFYTTLFY