AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05846

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05846.t1 MTR_3g067615 73.684 95 23 1 19 111 3 97 1.60e-43 143
MS.gene05846.t1 MTR_3g066090 62.931 116 39 1 1 112 1 116 5.79e-43 138
MS.gene05846.t1 MTR_5g027890 60.714 112 44 0 1 112 1 112 1.11e-42 140
MS.gene05846.t1 MTR_3g067630 64.423 104 37 0 8 111 8 111 2.98e-42 141
MS.gene05846.t1 MTR_3g067625 63.063 111 41 0 1 111 1 111 4.72e-41 141
MS.gene05846.t1 MTR_3g067625 56.098 82 23 2 8 87 382 452 2.89e-20 84.7
MS.gene05846.t1 MTR_1g045100 62.931 116 37 3 2 111 3 118 3.07e-40 135
MS.gene05846.t1 MTR_1g044795 60.684 117 40 2 1 111 1 117 4.35e-38 129
MS.gene05846.t1 MTR_3g067645 61.947 113 41 2 1 112 2 113 2.89e-37 129
MS.gene05846.t1 MTR_3g080330 59.048 105 41 1 10 112 2 106 4.84e-36 123
MS.gene05846.t1 MTR_8g091730 52.066 121 48 3 1 111 2 122 2.57e-35 122
MS.gene05846.t1 MTR_3g080350 66.316 95 30 1 19 111 63 157 5.59e-35 119
MS.gene05846.t1 MTR_3g080310 59.615 104 40 1 10 111 2 105 8.72e-35 120
MS.gene05846.t1 MTR_6g015890 55.357 112 41 2 8 112 7 116 3.76e-34 119
MS.gene05846.t1 MTR_3g065820 54.867 113 49 1 1 111 1 113 7.82e-34 119
MS.gene05846.t1 MTR_3g065620 57.143 112 43 2 1 112 1 107 1.59e-33 117
MS.gene05846.t1 MTR_6g015900 53.571 112 43 2 8 112 4 113 1.05e-32 115
MS.gene05846.t1 MTR_5g036740 51.724 116 39 2 10 112 2 113 2.81e-32 114
MS.gene05846.t1 MTR_8g040130 56.566 99 37 1 19 111 8 106 6.53e-32 112
MS.gene05846.t1 MTR_3g080280 58.095 105 42 2 7 111 12 114 2.74e-30 109
MS.gene05846.t1 MTR_8g006980 54.717 106 46 1 8 111 6 111 2.04e-29 107
MS.gene05846.t1 MTR_5g062080 46.429 112 49 1 1 112 1 101 7.94e-29 104
MS.gene05846.t1 MTR_3g067990 48.760 121 41 4 3 111 2 113 1.44e-28 104
MS.gene05846.t1 MTR_3g065590 41.228 114 64 2 1 112 1 113 6.51e-27 101
MS.gene05846.t1 MTR_3g065850 53.333 105 46 2 9 111 7 110 1.01e-26 99.8
MS.gene05846.t1 MTR_3g065610 40.351 114 65 2 1 112 1 113 2.05e-26 99.8
MS.gene05846.t1 MTR_3g080360 46.429 112 59 1 1 111 21 132 2.12e-25 96.7
MS.gene05846.t1 MTR_3g065890 57.971 69 29 0 6 74 1 69 1.41e-24 93.6
MS.gene05846.t1 MTR_2g095850 45.794 107 31 1 5 111 3 82 9.51e-24 90.5
MS.gene05846.t1 MTR_7g047530 64.615 65 23 0 3 67 5 69 2.96e-22 87.0
MS.gene05846.t1 MTR_7g451730 64.615 65 23 0 3 67 14 78 3.49e-22 87.0
MS.gene05846.t1 MTR_3g437220 51.316 76 35 1 8 81 7 82 1.01e-20 82.4
MS.gene05846.t1 MTR_3g036480 51.316 76 35 1 8 81 6 81 4.10e-20 81.3
MS.gene05846.t1 MTR_2g018950 45.263 95 49 2 20 112 23 116 3.16e-18 78.2
MS.gene05846.t1 MTR_3g080270 45.631 103 31 1 10 112 2 79 8.67e-18 75.9
MS.gene05846.t1 MTR_2g018910 43.750 96 50 2 20 112 27 121 1.21e-17 76.6
MS.gene05846.t1 MTR_8g044970 58.333 60 24 1 52 111 9 67 2.88e-16 67.8
MS.gene05846.t1 MTR_2g018940 42.553 94 52 2 20 111 24 117 8.16e-16 71.6
MS.gene05846.t1 MTR_2g019060 38.298 94 57 1 20 112 27 120 3.75e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05846.t1 AT4G05460 46.018 113 57 2 1 111 1 111 1.09e-20 84.7
MS.gene05846.t1 AT4G05475 41.304 92 53 1 20 111 39 129 3.19e-15 69.7
MS.gene05846.t1 AT4G05470 41.935 93 52 2 20 111 45 136 9.70e-15 68.6
MS.gene05846.t1 AT4G05497 38.144 97 55 2 20 111 20 116 2.91e-12 61.2

Find 0 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTTCAACTAAATCGTCATC+TGG + 23890:5176-5195 MS.gene05846:intergenic 30.0%
! TGCACTTGTTACAATTTCAA+TGG + 23890:5069-5088 MS.gene05846:intergenic 30.0%
ATCTCATTCTATTCCTGCAA+AGG - 23890:4959-4978 MS.gene05846:CDS 35.0%
TCACTGCAAACATTCTTCAA+AGG - 23890:5036-5055 MS.gene05846:CDS 35.0%
AAAGCATAGCAGTGCCAAAT+TGG - 23890:4994-5013 MS.gene05846:CDS 40.0%
ATGACCAGAACTACGTTCAA+TGG + 23890:5210-5229 MS.gene05846:intergenic 40.0%
ATGTTTGCAGTGATGTCTCT+AGG + 23890:5029-5048 MS.gene05846:intergenic 40.0%
CATGTCAAGTTTGTCCTCAA+TGG - 23890:5087-5106 MS.gene05846:CDS 40.0%
CTAGGAAGTTGCAACCAATT+TGG + 23890:5011-5030 MS.gene05846:intergenic 40.0%
TATGCCATTGAACGTAGTTC+TGG - 23890:5203-5222 MS.gene05846:CDS 40.0%
TTGCATATGTTCCACCATTG+AGG + 23890:5104-5123 MS.gene05846:intergenic 40.0%
! TCAATGGTGGAACATATGCA+AGG - 23890:5103-5122 MS.gene05846:CDS 40.0%
GTCAAGTTTGTCCTCAATGG+TGG - 23890:5090-5109 MS.gene05846:CDS 45.0%
TATGCAAGGATCCTCTCATG+TGG - 23890:5117-5136 MS.gene05846:CDS 45.0%
TCACATTCTGCATCCTTTGC+AGG + 23890:4975-4994 MS.gene05846:intergenic 45.0%
! AATCGTCATCTGGAGTGTCA+AGG + 23890:5166-5185 MS.gene05846:intergenic 45.0%
CGAATAGTTCGCCACATGAG+AGG + 23890:5131-5150 MS.gene05846:intergenic 50.0%


Chromosome Type Strat End Strand Name
23890 gene 4954 5291 4954 ID=MS.gene05846
23890 mRNA 4954 5291 4954 ID=MS.gene05846.t1;Parent=MS.gene05846
23890 exon 4954 5291 4954 ID=MS.gene05846.t1.exon1;Parent=MS.gene05846.t1
23890 CDS 4954 5291 4954 ID=cds.MS.gene05846.t1;Parent=MS.gene05846.t1
Gene Sequence

>MS.gene05846

ATGGCATCTCATTCTATTCCTGCAAAGGATGCAGAATGTGAAAGCATAGCAGTGCCAAATTGGTTGCAACTTCCTAGAGACATCACTGCAAACATTCTTCAAAGGCTTGATACCATTGAAATTGTAACAAGTGCATGTCAAGTTTGTCCTCAATGGTGGAACATATGCAAGGATCCTCTCATGTGGCGAACTATTCGCATGATTGATGACCTTGACACTCCAGATGACGATTTAGTTGAAATTTGTCGCTATGCCATTGAACGTAGTTCTGGTCATCTAGAAATCATCGAAATTGAGTCTTTTTGCAACGACGATCTCCTTGAATGCATAGCTAAGAA
Protein sequence

>MS.gene05846.t1

MASHSIPAKDAECESIAVPNWLQLPRDITANILQRLDTIEIVTSACQVCPQWWNICKDPLMWRTIRMIDDLDTPDDDLVEICRYAIERSSGHLEIIEIESFCNDDLLECIAK