Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058504.t1 | RHN57038.1 | 98.6 | 486 | 7 | 0 | 1 | 486 | 131 | 616 | 5.20E-293 | 1016.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058504.t1 | Q0WSN2 | 67.4 | 494 | 146 | 6 | 4 | 486 | 39 | 528 | 2.3e-196 | 686.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058504.t1 | G7K212 | 98.6 | 486 | 7 | 0 | 1 | 486 | 19 | 504 | 3.8e-293 | 1016.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058504 | MS.gene058506 | 0.983096 | 8.11E-157 | -1.69E-46 |
| MS.gene058504 | MS.gene29548 | 0.932462 | 8.22E-95 | -1.69E-46 |
| MS.gene058504 | MS.gene29550 | 0.948831 | 4.32E-107 | -1.69E-46 |
| MS.gene058504 | MS.gene29551 | 0.977888 | 1.09E-144 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAAGCAAACTTCACATTT+TGG | 0.126071 | 5.3:+65574879 | None:intergenic |
| ATATGTGTATATTACCTTTA+AGG | 0.133013 | 5.3:+65568580 | None:intergenic |
| AGGCATAAACCTCTTGTTTC+TGG | 0.186251 | 5.3:+65569079 | None:intergenic |
| GTCTCATGTTGAAAACAAAT+TGG | 0.214527 | 5.3:-65568333 | MS.gene058504:CDS |
| TTAAAACAAGATTTGTGATA+TGG | 0.216060 | 5.3:+65574915 | None:intergenic |
| CTGTATTCATTGGGATAAAA+TGG | 0.256062 | 5.3:+65575261 | None:intergenic |
| ACTACCTCGCGCTCGGTAAT+AGG | 0.256980 | 5.3:+65575046 | None:intergenic |
| AATGAAGCTGTTAATAAATA+TGG | 0.258522 | 5.3:-65568242 | MS.gene058504:CDS |
| ACAGCAATTCTTGTTCTATA+TGG | 0.261118 | 5.3:-65568831 | MS.gene058504:CDS |
| CATGAGATGGACAATACTTT+TGG | 0.265205 | 5.3:+65574260 | None:intergenic |
| CATGCATGGTTACGCCTTAA+AGG | 0.286050 | 5.3:-65568594 | MS.gene058504:intron |
| AGTATAGATGCCACCCCTTT+TGG | 0.293022 | 5.3:-65574285 | MS.gene058504:CDS |
| GGAGAAAATGATGAAGATTA+TGG | 0.298017 | 5.3:-65575339 | MS.gene058504:CDS |
| AATGATTCTTCTCCAGCTTA+TGG | 0.306927 | 5.3:-65568284 | MS.gene058504:CDS |
| ATTGGAGGCCATAGACTTAT+AGG | 0.316467 | 5.3:-65568897 | MS.gene058504:CDS |
| AAGATTTCACCTGTATTCAT+TGG | 0.335163 | 5.3:+65575251 | None:intergenic |
| ATCAAACCAGCAGCATTTAT+AGG | 0.343784 | 5.3:+65574310 | None:intergenic |
| GAAAGTCTGGTTTCATGAAA+TGG | 0.348540 | 5.3:-65576315 | MS.gene058504:CDS |
| AACAAGAGGTTTATGCCTTT+CGG | 0.351720 | 5.3:-65569074 | MS.gene058504:CDS |
| ACGACCACTCCTTCCTCTAT+TGG | 0.353168 | 5.3:+65576267 | None:intergenic |
| GTGCATGGAATCTGCTATAA+TGG | 0.356084 | 5.3:-65569335 | MS.gene058504:CDS |
| ATAATCTATGGAAATTGTTT+AGG | 0.366827 | 5.3:-65575124 | MS.gene058504:CDS |
| AGTCTATGGCCTCCAATTCT+TGG | 0.369796 | 5.3:+65568903 | None:intergenic |
| CAGAAGGAGAAAGAGGATTT+AGG | 0.374040 | 5.3:-65575528 | MS.gene058504:CDS |
| GCACTTAATGATGCTATTGT+TGG | 0.375277 | 5.3:-65569208 | MS.gene058504:CDS |
| ATGAAACCAGACTTTCTCTC+TGG | 0.376624 | 5.3:+65576322 | None:intergenic |
| CCATGCAATGTCACTTTCTT+CGG | 0.377621 | 5.3:-65575505 | MS.gene058504:CDS |
| TTCTCATGCCTATAAGTCTA+TGG | 0.380772 | 5.3:+65568889 | None:intergenic |
| AACCTCTTGTTTCTGGCAAA+TGG | 0.381781 | 5.3:+65569086 | None:intergenic |
| CATTAGCCCATCAAGGATAT+AGG | 0.386946 | 5.3:-65575368 | MS.gene058504:intron |
| ATGGCATGCAGGTTACTAAC+AGG | 0.396063 | 5.3:-65568642 | MS.gene058504:intron |
| CTCGAACATATTCGTTTGAC+TGG | 0.400738 | 5.3:-65568206 | MS.gene058504:CDS |
| CATAATAATCTCTGATTGAA+TGG | 0.404836 | 5.3:+65569287 | None:intergenic |
| CTGTAGTTGTGAAAGATTAG+AGG | 0.408619 | 5.3:-65574215 | MS.gene058504:intron |
| TGCAATCAGGAGATAATCTA+TGG | 0.410658 | 5.3:-65575136 | MS.gene058504:CDS |
| TGTTGTTTCAGATTCATAGA+AGG | 0.415721 | 5.3:-65568926 | MS.gene058504:intron |
| TTGTTTACCAAAGCTCTAGA+TGG | 0.420805 | 5.3:+65576202 | None:intergenic |
| GAATCTGCTATAATGGATAC+TGG | 0.421279 | 5.3:-65569328 | MS.gene058504:CDS |
| ATTCATAGAAGGCCAAGAAT+TGG | 0.426756 | 5.3:-65568915 | MS.gene058504:CDS |
| AGATTTCACCTGTATTCATT+GGG | 0.428419 | 5.3:+65575252 | None:intergenic |
| CATATGCCTTCCTCTACTTC+AGG | 0.435073 | 5.3:+65568467 | None:intergenic |
| TTCAAAGGTGGGTCCAATAG+AGG | 0.438064 | 5.3:-65576280 | MS.gene058504:CDS |
| TCAATCAGAGATTATTATGA+AGG | 0.445893 | 5.3:-65569283 | MS.gene058504:CDS |
| CAAGACATGCCTTCCTCTGC+AGG | 0.450171 | 5.3:+65576232 | None:intergenic |
| GTCAGGTTCTGTCATTTATG+TGG | 0.451592 | 5.3:-65568445 | MS.gene058504:CDS |
| TCTTCTCCAGCTTATGGTGG+TGG | 0.452845 | 5.3:-65568278 | MS.gene058504:CDS |
| TCTCATGTTGAAAACAAATT+GGG | 0.452858 | 5.3:-65568332 | MS.gene058504:CDS |
| GCGTGCTCCATCTAGAGCTT+TGG | 0.454192 | 5.3:-65576209 | MS.gene058504:intron |
| TAAAATGGTGCAGGAGCATA+AGG | 0.458370 | 5.3:+65575276 | None:intergenic |
| TCACACCCAGAGAGAAAGTC+TGG | 0.460029 | 5.3:-65576328 | MS.gene058504:CDS |
| CAGATTCCTATAAATGCTGC+TGG | 0.468618 | 5.3:-65574316 | MS.gene058504:CDS |
| AGACTTTGCACCTTTCTTTG+AGG | 0.470152 | 5.3:+65568352 | None:intergenic |
| GAGTGGCATCGAAAGCAGTC+TGG | 0.476246 | 5.3:+65575079 | None:intergenic |
| ATGCAATGAGAGTTTGTTGT+TGG | 0.495239 | 5.3:+65568404 | None:intergenic |
| TGGAAATTGTTTAGGCTGCA+TGG | 0.497463 | 5.3:-65575116 | MS.gene058504:CDS |
| GAAGATATGAAGAGACCAAA+TGG | 0.499622 | 5.3:-65575480 | MS.gene058504:intron |
| TTCTCTTTCAACAAGAAGCA+TGG | 0.501914 | 5.3:+65569231 | None:intergenic |
| CTAAAACCACCACCATAAGC+TGG | 0.509878 | 5.3:+65568272 | None:intergenic |
| CTGCAGAGGAAGGCATGTCT+TGG | 0.516391 | 5.3:-65576231 | MS.gene058504:CDS |
| TAGAGCTCAGAAGGAGAAAG+AGG | 0.516816 | 5.3:-65575535 | MS.gene058504:CDS |
| ACCATGCATGCATCAACTCA+TGG | 0.520688 | 5.3:+65568607 | None:intergenic |
| TGATCGTGCTAGAGCTCAGA+AGG | 0.527771 | 5.3:-65575544 | MS.gene058504:CDS |
| TTATTTGCATTAGCCCATCA+AGG | 0.527904 | 5.3:-65575375 | MS.gene058504:intron |
| TCTCTGATTGAATGGTATAG+AGG | 0.533543 | 5.3:+65569295 | None:intergenic |
| GGATCAAACTACCTCGCGCT+CGG | 0.536169 | 5.3:+65575039 | None:intergenic |
| GCTGCTTCTTCCTCAAAGAA+AGG | 0.536522 | 5.3:-65568362 | MS.gene058504:CDS |
| CATAGAAGGCCAAGAATTGG+AGG | 0.541624 | 5.3:-65568912 | MS.gene058504:CDS |
| TCTCTTTCAACAAGAAGCAT+GGG | 0.545613 | 5.3:+65569232 | None:intergenic |
| AAGCTTGTGCTTAGAGTGCA+TGG | 0.547235 | 5.3:-65569350 | MS.gene058504:CDS |
| CCCATCAAGGATATAGGTGG+GGG | 0.548766 | 5.3:-65575362 | MS.gene058504:CDS |
| CACCATTTGCCAGAAACAAG+AGG | 0.548780 | 5.3:-65569088 | MS.gene058504:CDS |
| TCATTGGGATAAAATGGTGC+AGG | 0.553987 | 5.3:+65575267 | None:intergenic |
| TGAAACCAGACTTTCTCTCT+GGG | 0.553994 | 5.3:+65576323 | None:intergenic |
| CATTATGATCCTGCAGAGGA+AGG | 0.555932 | 5.3:-65576241 | MS.gene058504:CDS |
| AACAACAAACTCTCATTGCA+TGG | 0.560546 | 5.3:-65568402 | MS.gene058504:CDS |
| CCGAAGAAAGTGACATTGCA+TGG | 0.560644 | 5.3:+65575505 | None:intergenic |
| AACATGTGTTCTTATTACAC+AGG | 0.561919 | 5.3:+65568176 | None:intergenic |
| GATTCTTCTCCAGCTTATGG+TGG | 0.562395 | 5.3:-65568281 | MS.gene058504:CDS |
| AGTAGAGGAAGGCATATGTC+AGG | 0.562990 | 5.3:-65568462 | MS.gene058504:CDS |
| CTATAGTATTTCGTATTCCG+AGG | 0.568127 | 5.3:+65569388 | None:intergenic |
| CCATGCATGCATCAACTCAT+GGG | 0.575521 | 5.3:+65568608 | None:intergenic |
| AGCCCATCAAGGATATAGGT+GGG | 0.576893 | 5.3:-65575364 | MS.gene058504:CDS |
| CCCATGAGTTGATGCATGCA+TGG | 0.580940 | 5.3:-65568608 | MS.gene058504:CDS |
| TCACAGTTTGCTCTTCCGAA+AGG | 0.583186 | 5.3:+65569059 | None:intergenic |
| AAATGATGAAGATTATGGAA+AGG | 0.584158 | 5.3:-65575334 | MS.gene058504:CDS |
| GTATTGTCATATTCATGAGA+TGG | 0.585321 | 5.3:+65574247 | None:intergenic |
| GCCCATCAAGGATATAGGTG+GGG | 0.591148 | 5.3:-65575363 | MS.gene058504:CDS |
| CAATCTCAGTCCTGAAGTAG+AGG | 0.592599 | 5.3:-65568477 | MS.gene058504:CDS |
| TGAGGAAGAAGCAGCAGCTG+TGG | 0.594186 | 5.3:+65568370 | None:intergenic |
| TAGCCCATCAAGGATATAGG+TGG | 0.597899 | 5.3:-65575365 | MS.gene058504:CDS |
| CATCAAGGATATAGGTGGGG+GGG | 0.609524 | 5.3:-65575360 | MS.gene058504:CDS |
| CATTGAACAGAAGACTATGT+GGG | 0.609543 | 5.3:-65575162 | MS.gene058504:intron |
| AATACTATAGACTCGAGGAC+GGG | 0.611229 | 5.3:-65569375 | MS.gene058504:CDS |
| CTCAGTCCTGAAGTAGAGGA+AGG | 0.619865 | 5.3:-65568473 | MS.gene058504:CDS |
| AATGGTGCAGGAGCATAAGG+AGG | 0.625823 | 5.3:+65575279 | None:intergenic |
| TTCTCCTATTACCGAGCGCG+AGG | 0.631372 | 5.3:-65575050 | MS.gene058504:intron |
| AAGGTGGGTCCAATAGAGGA+AGG | 0.645391 | 5.3:-65576276 | MS.gene058504:CDS |
| ATTGAACAGAAGACTATGTG+GGG | 0.648467 | 5.3:-65575161 | MS.gene058504:intron |
| AAATACTATAGACTCGAGGA+CGG | 0.654015 | 5.3:-65569376 | MS.gene058504:CDS |
| GCATTGAACAGAAGACTATG+TGG | 0.658253 | 5.3:-65575163 | MS.gene058504:intron |
| TTGTTCTATATGGTCTACCA+AGG | 0.659611 | 5.3:-65568821 | MS.gene058504:intron |
| ACATGTGTTCTTATTACACA+GGG | 0.664721 | 5.3:+65568177 | None:intergenic |
| GGGTCCAATAGAGGAAGGAG+TGG | 0.672837 | 5.3:-65576271 | MS.gene058504:CDS |
| ATGGTGCAGGAGCATAAGGA+GGG | 0.678829 | 5.3:+65575280 | None:intergenic |
| CCATCAAGGATATAGGTGGG+GGG | 0.680331 | 5.3:-65575361 | MS.gene058504:CDS |
| TAATAGGAGAATGACAAGAG+TGG | 0.687598 | 5.3:+65575062 | None:intergenic |
| TACGAAATACTATAGACTCG+AGG | 0.695310 | 5.3:-65569380 | MS.gene058504:CDS |
| TCATCATTATGATCCTGCAG+AGG | 0.695986 | 5.3:-65576245 | MS.gene058504:CDS |
| TTGAACAGAAGACTATGTGG+GGG | 0.724066 | 5.3:-65575160 | MS.gene058504:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 65568190 | 65576364 | 65568190 | ID=MS.gene058504 |
| chr5.3 | mRNA | 65568190 | 65576364 | 65568190 | ID=MS.gene058504.t1;Parent=MS.gene058504 |
| chr5.3 | exon | 65576210 | 65576364 | 65576210 | ID=MS.gene058504.t1.exon1;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65576210 | 65576364 | 65576210 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65575481 | 65575568 | 65575481 | ID=MS.gene058504.t1.exon2;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65575481 | 65575568 | 65575481 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65575261 | 65575384 | 65575261 | ID=MS.gene058504.t1.exon3;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65575261 | 65575384 | 65575261 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65575051 | 65575174 | 65575051 | ID=MS.gene058504.t1.exon4;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65575051 | 65575174 | 65575051 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65574875 | 65574958 | 65574875 | ID=MS.gene058504.t1.exon5;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65574875 | 65574958 | 65574875 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65574216 | 65574335 | 65574216 | ID=MS.gene058504.t1.exon6;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65574216 | 65574335 | 65574216 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65569198 | 65569410 | 65569198 | ID=MS.gene058504.t1.exon7;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65569198 | 65569410 | 65569198 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65569054 | 65569116 | 65569054 | ID=MS.gene058504.t1.exon8;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65569054 | 65569116 | 65569054 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65568822 | 65568937 | 65568822 | ID=MS.gene058504.t1.exon9;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65568822 | 65568937 | 65568822 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65568595 | 65568653 | 65568595 | ID=MS.gene058504.t1.exon10;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65568595 | 65568653 | 65568595 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| chr5.3 | exon | 65568190 | 65568506 | 65568190 | ID=MS.gene058504.t1.exon11;Parent=MS.gene058504.t1 |
| chr5.3 | CDS | 65568190 | 65568506 | 65568190 | ID=cds.MS.gene058504.t1;Parent=MS.gene058504.t1 |
| Gene Sequence |
| Protein sequence |
